Read by QxMD icon Read


Han Zhu, Hua Qin, De-Min Li, Jing Liu, Qiu Zhao
The objective of this study was to investigate the effect of silencing gene protein phosphatase 1H (PPM1H) on malignant phenotype of human pancreatic cancer cell line BxPC-3. In order to explore the function of PPM1H in pancreatic cancer cells, real-time PCR and western blotting were used to detect the expression of PPM1H in different pancreatic cancer cell lines. Human pancreatic cancer cell line BxPC-3 was treated with 10 ng/ml TGF-β1 and 200 ng/ml BMP2 for 72 h, respectively, and the mRNA and protein expression levels of PPM1H and EMT-related markers (E-cadherin, vimentin) were detected by real-time PCR and western blotting, respectively...
September 5, 2016: Oncology Reports
Menglin Xu, Diane C Wang, Xiangdong Wang, Yong Zhang
Mucins (MUC) are a family consisting of large O-glycoproteins whose primary functions are to protect and lubricate cell epithelial surfaces and contribute to intra- and inter-cellular signal pathways, cell proliferation, growth and apotosis. With the development of new technologies, MUCs begin to be identified as an effective marker in evaluating the tumor heterogeneity in lung cancer. MUCs' diverse expressions in subtypes of lung cancer indicate the inter-tumor heterogeneity. MUCs' mutation may also contribute to the development of intra-heterogeneity and evolution of lung cancer...
August 25, 2016: Seminars in Cell & Developmental Biology
Xiao-Xia Sun, Liang-Ming Ma, Tao Wang
OBJECTIVE: To investigate the effect and possible mechanism of bromo-domain inhibitors (JQ1) on proliferation inhibition and inducing apoptosis of acute T lymphocyte leukemia cell line (Jurkat) . METHODS: Jurkat cell line was treated by JQ1 at different concentrations. MTT was used to detect the cell proliferation inhibition rate. The flow cytometry with AnnexinV-FITC/PI fluorescence staining was used to detect the changes of apoptosis rate, and real-time fluorescent quantitative PCR was used to detect c-Myc/Notch1 gene expression levels...
August 2016: Zhongguo Shi Yan Xue Ye Xue za Zhi
Jun-Shuai Yue, Qing-Yun Wu, Ling-Yu Zenge, Kai-Lin Xu
OBJECTIVE: To detect the expression of PTPN11 gene in acute myeloid leukemia (AML) cell line and to explore the effects of PTPN11 over expressing on proliferation and apotosis of AML cell lines. METHODS: The expression of PTPN11 in AML cell lines(HEL,U937, K562, KG-1, HL -60) was detected by RT-PCR, Q-PCR and Western blot. The PTPN11 gene was amplified by RT-PCR. PTPN11 DNA fragement and the lentiviral vector PCDH-CD513B were digested by BamHI and EcoRI, and then ligated by T4 DNA ligase...
August 2016: Zhongguo Shi Yan Xue Ye Xue za Zhi
Xiaohong Li, Ying Xiao, Shaoqing Fan, Mingbing Xiao, Xiaotong Wang, Xudong Chen, Chunsun Li, Guijuan Zong, Guoxiong Zhou, Chunhua Wan
OBJECTIVES: The receptor for activated protein kinase C (RACK1) is a scaffold protein involved in multiple intracellular signal pathways. Previous studies have shown that RACK1 is associated with the progression of multiple cancer types, including hepatocellular carcinoma and gastric cancer. However, the role of RACK1 in human pancreatic ductal adenocarcinoma (PDAC) remains unclear. METHODS: In this study, the expression of RACK1 was evaluated by Western blot analysis in 8 paired fresh PDAC tissues and immunohistochemistry on 179 paraffin-embedded slices...
August 3, 2016: Experimental and Molecular Pathology
Qiuping Chen, Tongda Xu, Dongye Li, Defeng Pan, Pei Wu, Yuanyuan Luo, Yanfeng Ma, Yang Liu
Recent studies have demonstrated that diabetes impairs the phosphatidylinositol 3-kinase/Akt (PI3K/Akt) pathway, while insulin resistance syndrome has been associated with alterations of this pathway in diabetic rats after ischemia/reperfusion (I/R), and activation of C-jun N-terminal kinase (JNK) is involved. The present study was designed to investigate whether inhibiting JNK activity would partially restore the PI3K/Akt signaling pathway and protect against myocardial I/R injury in diabetic rats, and to explore the effect of intervention with salvianolic acid A (Sal A)...
2016: American Journal of Translational Research
Chuanxiong Nie, Chong Cheng, Lang Ma, Jie Deng, Changsheng Zhao
Nanointerfacial decoration of silver nanoparticles (AgNPs) is an ideal protocol to improve the antibacterial efficiency of diverse nanomaterials, including carbon nanotube (CNT), graphene, and many other intensively studied nanoarchitectures, which provides a tremendous possibility for designing advanced antibacterial biomaterials and biomedical devices. However, the direct exposure of AgNPs will lead to potential mammalian cell apoptosis and death, which significantly limits their biological applications. In this study, we demonstrated a green and one-step approach to achieve robust antibacterial and highly biocompatible AgNP-CNT composites...
June 14, 2016: Langmuir: the ACS Journal of Surfaces and Colloids
A V Pronin, I V Gogoleva, I Yu Torshin, O A Gromova
For over 60 years, high doses of lithium (hundreds of milligrams of elemental lithium) have being used to treat bipolar disorder. However, only during the past 20 years the relevant basic and clinical studies have shown that neuroprotective and neurotrophic effects of lithium are possible in much smaller doses ( hundreds of micrograms of elemental lithium). These data indicate a significant potential for the clinical applications of lithium-based drugs in modern neurology for the purposes of prevention and treatment of neurodegenerative and ischemic pathologies...
2016: Zhurnal Nevrologii i Psikhiatrii Imeni S.S. Korsakova
Yating Liu, Tao Li, Yuanhong Xu, Enjun Xu, Min Zhou, Baolong Wang, Jilong Shen
Toll-like receptors (TLRs) are key factors in the innate immune system and initiate an inflammatory response to foreign pathogens, such as bacteria, fungi and viruses. TLR4-mediated signaling has been implicated in tumor cell proliferation and apoptosis in numerous cancers. The present study aimed to investigate the biological effect of TLR4 on the proliferation and apoptosis of human liver cancer cells and the mechanisms responsible for the regulation of cellular responses following TLR4 gene knockdown. Three TLR4 small interfering (si)RNA constructs, consisting of TLR4-siRNA-1, TLR4-siRNA-2 and TLR4-siRNA-3, were transiently transfected into HepG2 cells using Lipofectamine 2000...
May 2016: Oncology Letters
Zifeng Wang, Na Liu, Shuo Shi, Sanhong Liu, Haifan Lin
P-element-induced wimpy testis (PIWI) proteins bind to PIWI-interacting RNAs and play key roles in the biogenesis and functions of PIWI-interacting RNAs. It has been reported that PIWI proteins are essential for stem cell self-renewal and germline development in diverse organisms and that they are ectopically expressed in multiple forms of cancer. However, the role of PIWI in cancer remains elusive. Here we report that one of the four PIWI proteins in humans, PIWIL4, is highly expressed in both breast cancer tissues and the cytoplasm of MDA-MB-231 cells derived from breast cancer...
May 13, 2016: Journal of Biological Chemistry
Cristina Talarico, Lucia D'Antona, Domenica Scumaci, Agnese Barone, Francesco Gigliotti, Claudia Vincenza Fiumara, Vincenzo Dattilo, Enzo Gallo, Paolo Visca, Francesco Ortuso, Claudia Abbruzzese, Lorenzo Botta, Silvia Schenone, Giovanni Cuda, Stefano Alcaro, Cataldo Bianco, Patrizia Lavia, Marco G Paggi, Nicola Perrotti, Rosario Amato
The SGK1 kinase is pivotal in signal transduction pathways operating in cell transformation and tumor progression. Here, we characterize in depth a novel potent and selective pyrazolo[3,4-d]pyrimidine-based SGK1 inhibitor. This compound, named SI113, active in vitro in the sub-micromolar range, inhibits SGK1-dependent signaling in cell lines in a dose- and time-dependent manner. We recently showed that SI113 slows down tumor growth and induces cell death in colon carcinoma cells, when used in monotherapy or in combination with paclitaxel...
November 10, 2015: Oncotarget
Dengrui Li, Sumin Guo, Hui Li, Guiyun Zhu, Li Gao, Xin Xin, Dandan Yan, Xiuwu Li, Shujun Geng, Hongwei Hou, Yonghui Yang
BACKGROUND: Adenocarcinoma, the most common form of lung cancer, is one of main human malignant tumors. In this paper, we focus on the effect of antitumor activity of cytokine-induced killer (CIK) cells on human lung adenocarcinoma cell line A549. METHODS: CIK cells were obtained by inducing peripheral blood mononuclear cells with recombinant human (rh) interferon-gamma, monoclonal anti-CD3 antibody, rh interleukin (IL)-1alpha, and rhIL-2, which were added into the culture...
July 2015: Thoracic Cancer
Robert Hanna
This review summarises the findings of a series of studies in which the histological changes, induced in the reproductive system of Fasciola hepatica following treatment of the ovine host with the anthelmintic triclabendazole (TCBZ), were examined. A detailed description of the normal macroscopic arrangement and histological features of the testes, ovary, vitelline tissue, Mehlis' gland and uterus is provided to aid recognition of the drug-induced lesions, and to provide a basic model to inform similar toxicological studies on F...
2015: Pathogens
Yuqi Cui, Fengpeng Jia, Jianfeng He, Xiaoyun Xie, Zhihong Li, Minghuan Fu, Hong Hao, Ying Liu, Dylan Z Liu, Peter J Cowan, Hua Zhu, Qinghua Sun, Zhenguo Liu
AIMS: Some environmental insults, such as fine particulate matter (PM) exposure, significantly impair the function of stem cells. However, it is unknown if PM exposure could affect the population of bone marrow stem cells (BMSCs). The present study was to investigate the effects of PM on BMSCs population and related mechanism(s). MAIN METHEODS: PM was intranasally distilled into male C57BL/6 mice for one month. Flow cytometry with antibodies for BMSCs, Annexin V and BrdU ware used to determine the number of BMSCs and the levels of their apoptosis and proliferation in vivo...
2015: PloS One
Xue-Tao Li, Hang-Zhou Wang, Zhi-Wu Wu, Tian-Quan Yang, Zhao-Hui Zhao, Gui-Lin Chen, Xue-Shun Xie, Bin Li, Yong-Xin Wei, Yu-Lun Huang, You-Xin Zhou, Zi-Wei Du
Malignant gliomas are the most common primary brain tumors, and the molecular mechanisms involving their progression and recurrence are still largely unclear. Substantial data indicate that the oncogene miR-494-3p is significantly elevated in gliomas, but the molecular functions of miR-494-3p in gliomagenesis are largely unknown. The present study aimed to explore the role of miR-494-3p and its molecular mechanism in human brain gliomas, malignant glioma cell lines, and cancer stem-like cells. The expression level of miR-494-3p in 48 human glioma issues and 8 normal brain tissues was determined using stem-loop real-time polymerase chain reaction (PCR)...
July 2015: Cellular and Molecular Neurobiology
Juan Calderón-Colmenero, Julio Sandoval Zárate, Miguel Beltrán Gámez
Pulmonary arterial hypertension is a common complication of congenital heart disease (CHD). Congenital cardiopathies are the most frequent congenital malformations. The prevalence in our country remains unknown, based on birthrate, it is calculated that 12,000 to 16,000 infants in our country have some cardiac malformation. In patients with an uncorrected left-to-right shunt, increased pulmonary pressure leads to vascular remodeling and endothelial dysfunction secondary to an imbalance in vasoactive mediators which promotes vasoconstriction, inflammation, thrombosis, cell proliferation, impaired apotosis and fibrosis...
January 2015: Archivos de Cardiología de México
G-J Liu, G-J Pan, J Wang, L-N Wang, Y Xu, Y Tang
OBJECTIVE: To study the mechanism of early chemosensitivity and later chemoresistance of Cytarabine (Cyt) to HL-60 cells to regulate ERK/Slug signal pathway. MATERIALS AND METHODS: HL-60 cells were stably transfected with NF-κBp65 siRNA,P53 up-regulated modulator of apoptosis (PUMA) siRNA (PUMA siRNA) and Slug siRNA respectively or exposed to 25 nM U0126 for 24h, then the cells were exposed to Cyt (250 nM) respectively. In different time point after Cyt treatment, the activity of NF-κB and the expression of Erk1/2, NF-κBp65, Slug and PUMA protein were detected...
2014: European Review for Medical and Pharmacological Sciences
Yuhong Huang, Qing Wang, Yue Du, Lulu Bai, Fen Jin, Jun Zhang, Shujun Fan, Huaxin Wang, Lin Song, Yue Gao, Xianyan Wang, Jianwu Tang
Our previous studies have shown that annexin A7 (ANXA7) gives different expressions in the mouse hepatocarcinoma cell lines with low or high lymphatic metastatic potential in both gene and protein levels. In this study, whether by using RNA interference (RNAi) technique downregulating ANXA7 in the gene level or by using antibody against ANXA7 in the protein level, the depressed expression of ANXA7 could induce apotosis and decrease the invasion, migration capacities of the Hca-P cell, a hepatocarcinoma cell line with low lymphatic metastatic potential in vitro...
September 2014: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Xia Xie, Jie Li, Rui-Cang Wang, Rui-Li Geng, Su-Yun Wang, Chao Wang, Xiao-Yun Zhao, Hong-Ling Hao
This study was aimed to investigate the effect of COX-2 inhibitor celecoxib on proliferation, apoptosis of human acute myeloid leukemia cell line HL-60 and its mechanism. HL-60 cells were cultured with different concentrations of celecoxib for 24 h. Cell proliferation was analyzed by CCK-8 assay, cell apoptosis and cell cycle distribution were detected by flow cytometry. Cyclin D1, cyclin E1 and COX-2 mRNA expressions were determined by RT-PCR. The results showed that after the HL-60 cells were treated with different concentrations of celecoxib for 24 h, the cell growth was significantly inhibited in a dose-dependent manner(r = 0...
June 2014: Zhongguo Shi Yan Xue Ye Xue za Zhi
Shuo Geng, Xiaojun Zhang, Jie Chen, Xing Liu, Hepeng Zhang, Xiaoyan Xu, Yan Ma, Baoxin Li, Yunqi Zhang, Zhenggang Bi, Chenglin Yang
MicroRNAs have crucial roles in development and progression of human cancers, including osteosarcoma. Recent studies have shown that miR-124 was down-regulated in many cancers; however, the role of miR-124 in osteosarcoma development is unknown. In this study, we demonstrate that expression of miR-124 is significantly downregulated in osteosarcoma tissues and cell lines, compared to the adjacent tissues. The expression of miR-124 in the metastases osteosarcoma tissues was lower than that in non- metastases tissues...
2014: PloS One
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"