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Apotosis

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https://www.readbyqxmd.com/read/29057216/contribution-of-in-vivo-experimental-challenges-to-understanding-flat-oyster-ostrea-edulis-resistance-to-bonamia-ostreae
#1
Benjamin Morga, Tristan Renault, Nicole Faury, Sophie Lerond, Céline Garcia, Bruno Chollet, Jean-Pierre Joly, Sylvie Lapègue, Estelle Harrang, Isabelle Arzul
Bonamiosis due to the parasite Bonamia ostreae has been associated with massive mortality outbreaks in European flat oyster stocks in Europe. As eradication and treatment are not possible, the control of the disease mainly relies on transfer restriction. Moreover, selection has been applied to produce resistant flat oyster families, which present better survival and lower prevalence than non-selected oysters. In order to better understand the mechanisms involved in resistance to bonamiosis, cellular and molecular responses of 2 oyster groups (selected oysters and wild-type oysters) were analyzed in the context of experimental injection and cohabitation infections...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28960861/lentivirus-mediated-disintegrin-and-metalloproteinase-17-rna-interference-reversed-the-acquired-resistance-to-gefitinib-in-lung-adenocarcinoma-cells-in-vitro
#2
Ya-Qing Li, Yuan-Shun Liu, Xi-Wang Ying, Hong-Bin Zhou, Zhehua Wang, Sheng-Chang Wu, Jian-Ping Yan, Yu-Ting Jing, Yang Yang
OBJECTIVE: The aim of the study is to evaluate the effects of silencing a disintegrin and metalloproteinase 17 (ADAM17) gene expression by lentivirus-mediated RNA interference (RNAi) in the gefitinib-resistant lung adenocarcinoma cells, and then to explore whether the recombinant lentivirus mediated ADAM17 RNAi reversed the acquired resistance of lung adenocarcinoma to gefitinib in vitro. METHODS: The gefitinib-resistant RPC-9 cells were established and the mutations of EGFR were detected by gene sequencing...
September 27, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/28883899/caffeic-acid-phenethyl-ester-reduces-ischemia-induced-kidney-mitochondrial-injury-in-rats
#3
Sonata Trumbeckaite, Neringa Pauziene, Darius Trumbeckas, Mindaugas Jievaltas, Rasa Baniene
During partial nephrectomy, the avoidance of ischemic renal damage is extremely important as duration of renal artery clamping (i.e., ischemia) influences postoperative kidney function. Mitochondria (main producer of ATP in the cell) are very sensitive to ischemia and undergo damage during oxidative stress. Finding of a compound which diminishes ischemic injury to kidney is of great importance. Caffeic acid phenethyl ester (CAPE), biologically active compound of propolis, might be one of the promising therapeutic agents against ischemia-caused damage...
2017: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/28688122/diminazene-aceturate-prevents-nephropathy-by-increasing-glomerular-ace2-and-at2-receptor-expression-in-a-rat-model-of-type1-diabetes
#4
Santosh Kumar Goru, Almesh Kadakol, Vajir Malek, Anuradha Pandey, Nisha Sharma, Anil Bhanudas Gaikwad
BACKGROUND AND PURPOSE: One of the protective actions of angiotensin converting enzyme-2 (ACE2) is the inactivation of angiotensin II. Expression and activity of ACE2 was reduced in glomeruli of diabetic patients and in animal models of diabetes. Recently the potential role of recombinant ACE2 administration in preventing diabetic nephropathy (DN) has been shown. Here we have tested the effects of the ACE2 activator, diminazene aceturate (DIZE), in a model of DN. EXPERIMENTAL APPROACH: Male Wistar rats were rendered diabetic using a single dose of streptozotocin (55 mg·kg(-1) , i...
September 2017: British Journal of Pharmacology
https://www.readbyqxmd.com/read/28646900/human-umbilical-cord-stem-cell-conditioned-medium-versus-serum-free-culture-medium-in-the-treatment-of-cryopreserved-human-ovarian-tissues-in-in-vitro-culture-a-randomized-controlled-trial
#5
Yingxian Jia, Xiaohan Shi, Yidong Xie, Xiaochuan Xie, Yan Wang, Shangwei Li
BACKGROUND: To reduce young female fertility loss, the in-vitro culture of cryopreserved ovarian cortical tissues (OCTs) is considered an effective approach without delaying treatment and undergoing stimulation medicine. However, ischemic damage and follicular loss during the in-vitro culture of OCTs are major technical challenges. Human umbilical cord stem cells (HUMSCs) and their conditioned medium (HUMSC-CM) have been considered to be potential resources for regeneration medicine because they secrete cytokines and enhance cell survival and function...
June 24, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28638452/targeting-tpx2-suppresses-the-tumorigenesis-of-hepatocellular-carcinoma-cells-resulting-in-arrested-mitotic-phase-progression-and-increased-genomic-instability
#6
Chao-Wen Hsu, Yu-Chia Chen, Hsing-Hao Su, Guan-Jin Huang, Chih-Wen Shu, Tony Tong-Lin Wu, Hung-Wei Pan
Hepatocellular carcinoma (HCC) remains one of the most difficult cancers to treat, with chemotherapies being relatively ineffective. Therefore, a better knowledge of molecular hepatocarcinogenesis will provide opportunities for designing targeted therapies. TPX2 (targeting protein for Xklp2) is overexpressed as a consequence of oncogenic alterations and is likely to alter the proper regulation of chromosome segregation in cancer cells. Disrupting the machinery which is responsible for mitosis and chromosome instability in cancer cells can be one of the most successful strategies for cancer therapy...
2017: Journal of Cancer
https://www.readbyqxmd.com/read/28425856/apicidin-inhibited-proliferation-and-invasion-and-induced-apoptosis-via-mitochondrial-pathway-in-non-small-cell-lung-cancer-glc-82-cells
#7
Jianye Zhang, Zhenzhu Lai, Wenjing Huang, Huiping Ling, Minting Lin, Sili Tang, Yun Liu, Yiwen Tao
BACKGROUND: Apicidin, as an inhibitor of histone deacetylase, showed a wide range of antiproliferative activity against various cancer cell lines. Apicidin has also been reported to induce apotosis via Fas/Fas ligand. Yet few studies have been focused on mitochondrial pathway for its anti-tumor activity. OBJECTIVE: In this study, we evaluated its involved mitochondrial mechanism against non-small cell lung cancer GLC-82 cells. METHOD: Apicidin was isolated from the mangrove endophtic fungi Fusarium sp...
2017: Anti-cancer Agents in Medicinal Chemistry
https://www.readbyqxmd.com/read/28366952/oxidative-stress-and-apotosis-to-zebrafish-danio-rerio-embryos-exposed-to-perfluorooctane-sulfonate-pfos-and-zno-nanoparticles
#8
Jia Du, Jun Cai, Shutao Wang, Hong You
OBJECTIVES: Perfluorooctane sulfonate (PFOS) and zinc oxide nanoparticles (ZnO-NPs) are frequently detected in the environment but few studies have assessed the joint toxicity of them. Oxidative stress and apoptosis to zebrafish (Danio rerio) embryos induced by the PFOS and ZnO-NPs were investigated in this study. MATERIAL AND METHODS: The embryos were exposed to the PFOS (0, 0.4, 0.8 and 1.6 mg/l), ZnO-NPs (12.5, 25, 50 mg/l) and PFOS plus ZnO-NPs (0.4+12.5, 0...
March 30, 2017: International Journal of Occupational Medicine and Environmental Health
https://www.readbyqxmd.com/read/28363843/toll-like-receptor-9-mediates-paraquat-induced-acute-lung-injury-an-in-vitro-and-in-vivo-study
#9
Haitao Shen, Na Wu, Yu Wang, Lichun Zhang, Xiao Hu, Zhiguang Chen, Min Zhao
AIMS: This study aimed to investigate the role of Toll-like receptor 9 in paraquat-induced acute lung injury (ALI). MAIN METHODS: For in vivo study,C57BL mice were randomly assigned into the vehicle control group, paraquat group, paraquat + TLR9 antagonist (ODN2088) group, and TLR9 antagonist (ODN2088) group (n=36 per group). After paraquat 30mg/kg ip for 2, 24 and 48h, serum samples and lung tissues were collected to evaluate ALI and TLR9 signaling by lung injury score, protein levels of TLR9, MyD88, p-IRAK4, p-p65, and serum TNF-α and IL-1β levels...
June 1, 2017: Life Sciences
https://www.readbyqxmd.com/read/28245381/-effect-of-arsenic-trioxide-on-k562-cell-proliferation-and-its-mechanism
#10
Yuan Wang, Jie Yang, Jie Li, Rui-Cang Wang, Jun Yuan, Yan Li, Su-Yun Wang, Chao Wang, Hong-Ling Hao
OBJECTIVE: To investigate the molecular mechanism of arsenic trioxide(ATO) inhibiting K562 cell proliferation, and explore the new targets for treating chronic myeloid leukemia(CML). METHODS: human CML cell line K562 cells were cultured in vitro, and were treated with different concentrations of ATO; MTT was used to detect the cell proliferation; flow cytometry(FCM) was used to determine cell apoptosis, cell cycle and the expression of CD44; Transcriptional levels of β-catenin and cyclin D1 were assayed by RT-PCR...
February 2017: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://www.readbyqxmd.com/read/28196286/psoralen-with-ultraviolet-a-induced-apoptosis-of-cutaneous-lymphoma-cell-lines-is-augmented-by-type-i-interferons-via-the-jak1-stat1-pathway
#11
Walter Liszewski, David Gram Naym, Edyta Biskup, Robert Gniadecki
BACKGROUND: Photochemotherapy with psoralen and ultraviolet A (PUVA), with or without adjuvant interferon-α (IFN-α), is a first-line therapy for early-stage mycosis fungoides and other forms of cutaneous T-cell lymphoma (CTCL). However, the mechanism by which PUVA with IFN-α work in CTCL is poorly understood. PURPOSE: To develop a model to investigate the mechanisms of PUVA and PUVA with IFN-α in CTCL cells. METHODS: An in vitro model to study the molecular mechanisms of PUVA was created using two different CTCL cell lines, MyLa, which has functional p53, and HuT-78, in which p53 is inactivated due to a homozygous nonsense mutation...
May 2017: Photodermatology, Photoimmunology & Photomedicine
https://www.readbyqxmd.com/read/28181540/hiv-related-proteins-prolong-macrophage-survival-through-induction-of-triggering-receptor-expressed-on-myeloid-cells-1
#12
Zhihong Yuan, Xian Fan, Bashar Staitieh, Chetna Bedi, Paul Spearman, David M Guidot, Ruxana T Sadikot
Triggering receptor expressed on myeloid cells-1(TREM-1) is a member of the superimmunoglobulin receptor family. We have previously shown that TREM-1 prolongs survival of macrophages treated with lipoolysaccharide through Egr2-Bcl2 signaling. Recent studies suggest a role for TREM-1 in viral immunity. Human immunodeficiency virus-1 (HIV) targets the monocyte/macrophage lineage at varying stages of infection. Emerging data suggest that macrophages are key reservoirs for latent HIV even in individuals on antiretroviral therapy...
February 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28024482/-effects-of-homoharringtonine-combined-with-imatinib-on-k562-cell-apoptosis-and-bcl6-expression
#13
Qian Wang, Wei Ding, Jing-Jing Wu, Yi-Han Ding, Yu-Feng Li
OBJECTIVE: To explore the effects of homoharringtonine (HHT) alone or combined with imatinib (IM) on K562 cell proliferation and apoptosis, as well as the mRNA and protein expression of BCL6. METHODS: The CCK-8 was used to detect the inhibitory effect of drugs on cell growth, the flow cytometry was used to detect the cell apoptosis. The expression of BCL6 protein was assayed by Western blot, and BCL6 mRNA expression was detected by RT-PCR. RESULTS: HHT alone displayed a proliferation inhibition effect with dose- dependent manner, and induced apotosis; after combination of HHT and IM drugs, both the inhibitory rate and the apoptosis rate were significantly increased compared with the drug alone(P<0...
December 2016: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://www.readbyqxmd.com/read/27599670/effect-of-ppm1h-on-malignant-phenotype-of-human-pancreatic-cancer-cells
#14
Han Zhu, Hua Qin, De-Min Li, Jing Liu, Qiu Zhao
The objective of this study was to investigate the effect of silencing gene protein phosphatase 1H (PPM1H) on malignant phenotype of human pancreatic cancer cell line BxPC-3. In order to explore the function of PPM1H in pancreatic cancer cells, real-time PCR and western blotting were used to detect the expression of PPM1H in different pancreatic cancer cell lines. Human pancreatic cancer cell line BxPC-3 was treated with 10 ng/ml TGF-β1 and 200 ng/ml BMP2 for 72 h, respectively, and the mRNA and protein expression levels of PPM1H and EMT-related markers (E-cadherin, vimentin) were detected by real-time PCR and western blotting, respectively...
November 2016: Oncology Reports
https://www.readbyqxmd.com/read/27569189/correlation-between-mucin-biology-and-tumor-heterogeneity-in-lung-cancer
#15
REVIEW
Menglin Xu, Diane C Wang, Xiangdong Wang, Yong Zhang
Mucins (MUC) are a family consisting of large O-glycoproteins whose primary functions are to protect and lubricate cell epithelial surfaces and contribute to intra- and inter-cellular signal pathways, cell proliferation, growth and apotosis. With the development of new technologies, MUCs begin to be identified as an effective marker in evaluating the tumor heterogeneity in lung cancer. MUCs' diverse expressions in subtypes of lung cancer indicate the inter-tumor heterogeneity. MUCs' mutation may also contribute to the development of intra-heterogeneity and evolution of lung cancer...
April 2017: Seminars in Cell & Developmental Biology
https://www.readbyqxmd.com/read/27531767/-effect-of-brd4-inhibitor-jq1-on-proliferation-inhibition-and-apotosis-induction-in-jurkat-cells
#16
Xiao-Xia Sun, Liang-Ming Ma, Tao Wang
OBJECTIVE: To investigate the effect and possible mechanism of bromo-domain inhibitors (JQ1) on proliferation inhibition and inducing apoptosis of acute T lymphocyte leukemia cell line (Jurkat) . METHODS: Jurkat cell line was treated by JQ1 at different concentrations. MTT was used to detect the cell proliferation inhibition rate. The flow cytometry with AnnexinV-FITC/PI fluorescence staining was used to detect the changes of apoptosis rate, and real-time fluorescent quantitative PCR was used to detect c-Myc/Notch1 gene expression levels...
August 2016: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://www.readbyqxmd.com/read/27531757/-effects-of-ptpn11-on-the-biological-characteristics-of-aml-cells
#17
Jun-Shuai Yue, Qing-Yun Wu, Ling-Yu Zenge, Kai-Lin Xu
OBJECTIVE: To detect the expression of PTPN11 gene in acute myeloid leukemia (AML) cell line and to explore the effects of PTPN11 over expressing on proliferation and apotosis of AML cell lines. METHODS: The expression of PTPN11 in AML cell lines(HEL,U937, K562, KG-1, HL -60) was detected by RT-PCR, Q-PCR and Western blot. The PTPN11 gene was amplified by RT-PCR. PTPN11 DNA fragement and the lentiviral vector PCDH-CD513B were digested by BamHI and EcoRI, and then ligated by T4 DNA ligase...
August 2016: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://www.readbyqxmd.com/read/27498047/rack1-overexpression-associates-with-pancreatic-ductal-adenocarcinoma-growth-and-poor-prognosis
#18
Xiaohong Li, Ying Xiao, Shaoqing Fan, Mingbing Xiao, Xiaotong Wang, Xudong Chen, Chunsun Li, Guijuan Zong, Guoxiong Zhou, Chunhua Wan
OBJECTIVES: The receptor for activated protein kinase C (RACK1) is a scaffold protein involved in multiple intracellular signal pathways. Previous studies have shown that RACK1 is associated with the progression of multiple cancer types, including hepatocellular carcinoma and gastric cancer. However, the role of RACK1 in human pancreatic ductal adenocarcinoma (PDAC) remains unclear. METHODS: In this study, the expression of RACK1 was evaluated by Western blot analysis in 8 paired fresh PDAC tissues and immunohistochemistry on 179 paraffin-embedded slices...
October 2016: Experimental and Molecular Pathology
https://www.readbyqxmd.com/read/27398138/jnk-pi3k-akt-signaling-pathway-is-involved-in-myocardial-ischemia-reperfusion-injury-in-diabetic-rats-effects-of-salvianolic-acid-a-intervention
#19
Qiuping Chen, Tongda Xu, Dongye Li, Defeng Pan, Pei Wu, Yuanyuan Luo, Yanfeng Ma, Yang Liu
Recent studies have demonstrated that diabetes impairs the phosphatidylinositol 3-kinase/Akt (PI3K/Akt) pathway, while insulin resistance syndrome has been associated with alterations of this pathway in diabetic rats after ischemia/reperfusion (I/R), and activation of C-jun N-terminal kinase (JNK) is involved. The present study was designed to investigate whether inhibiting JNK activity would partially restore the PI3K/Akt signaling pathway and protect against myocardial I/R injury in diabetic rats, and to explore the effect of intervention with salvianolic acid A (Sal A)...
2016: American Journal of Translational Research
https://www.readbyqxmd.com/read/27189807/mussel-inspired-antibacterial-and-biocompatible-silver-carbon-nanotube-composites-green-and-universal-nanointerfacial-functionalization
#20
Chuanxiong Nie, Chong Cheng, Lang Ma, Jie Deng, Changsheng Zhao
Nanointerfacial decoration of silver nanoparticles (AgNPs) is an ideal protocol to improve the antibacterial efficiency of diverse nanomaterials, including carbon nanotube (CNT), graphene, and many other intensively studied nanoarchitectures, which provides a tremendous possibility for designing advanced antibacterial biomaterials and biomedical devices. However, the direct exposure of AgNPs will lead to potential mammalian cell apoptosis and death, which significantly limits their biological applications. In this study, we demonstrated a green and one-step approach to achieve robust antibacterial and highly biocompatible AgNP-CNT composites...
June 14, 2016: Langmuir: the ACS Journal of Surfaces and Colloids
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