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https://www.readbyqxmd.com/read/29674693/methylation-associated-silencing-of-basp1-contributes-to-leukemogenesis-in-t-8-21-acute-myeloid-leukemia
#1
Lei Zhou, Lin Fu, Na Lv, Jing Liu, Yan Li, Xiaosu Chen, Qingyu Xu, Guofeng Chen, Baoxu Pang, Lili Wang, Yonghui Li, Xiaodong Zhang, Li Yu
The AML1-ETO fusion protein (A/E), which results from the t(8;21) translocation, is considered to be a leukemia-initiating event. Identifying the mechanisms underlying the oncogenic activity of A/E remains a major challenge. In this study, we identified a specific down-regulation of brain acid-soluble protein 1 (BASP1) in t(8;21) acute myeloid leukemia (AML). A/E recognized AML1-binding sites and recruited DNA methyltransferase 3a (DNMT3a) to the BASP1 promoter sequence, which triggered DNA methylation-mediated silencing of BASP1...
April 20, 2018: Experimental & Molecular Medicine
https://www.readbyqxmd.com/read/29668012/evolution-of-dnmt2-in-drosophilids-evidence-for-positive-and-purifying-selection-and-insights-into-new-protein-pathways-interactions
#2
Gilberto Cavalheiro Vieira, Marícia Fantinel D'Ávila, Rebeca Zanini, Maríndia Deprá, Vera Lúcia da Silva Valente
The DNA methyltransferase 2 (DNMT2) protein is the most conserved member of the DNA methyltransferase family. Nevertheless, its substrate specificity is still controversial and elusive. The genomic role and determinants of DNA methylation are poorly understood in invertebrates, and several mechanisms and associations are suggested. In Drosophila, the only known DNMT gene is Dnmt2. Here we present our findings from a wide search for Dnmt2 homologs in 68 species of Drosophilidae. We investigated its molecular evolution, and in our phylogenetic analyses the main clades of Drosophilidae species were recovered...
2018: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/29662821/sperm-chromatin-and-dna-integrity-methyltransferase-mrna-levels-and-global-dna-methylation-in-oligoasthenoteratozoospermia
#3
Tahereh Rahiminia, Ehsan Farashahi Yazd, Farzaneh Fesahat, Mohammad Reza Moein, Ali Mohammad Mirjalili, Ali Reza Talebi
Objective: To investigate sperm chromatin/DNA integrity, global DNA methylation, and DNMT mRNA transcription in men with oligoasthenoteratozoospermia (OAT) compared with normozoospermic men. Methods: Semen samples from 32 OAT patients who comprised the case group and 32 normozoospermic men who comprised the control group were isolated and purified using a standard gradient isolation procedure according to World Health Organization criteria. DNMT1 , DNMT3A , and DNMT3B transcripts were then compared between groups using real-time quantitative reverse-transcription polymerase chain reaction...
March 2018: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/29642440/global-and-complement-gene-specific-dna-methylation-in-grass-carp-after-grass-carp-reovirus-gcrv-infection
#4
Lv Xiong, Libo He, Lifei Luo, Yongming Li, Lanjie Liao, Rong Huang, Zuoyan Zhu, Yaping Wang
Grass carp reovirus (GCRV) causes huge economic loss to the grass carp cultivation industry but the mechanism remains largely unknown. In this study, we investigated the global and complement gene-specific DNA methylation in grass carp after GCRV infection aimed to uncover the mechanism underlying GCRV infection. The global DNA methylation level was increased after GCRV infection. Expression levels of enzymes involved in DNA methylation including DNA methyltransferase (DNMT), ten-eleven translocation proteins (TETs), and glycine N -methyltransferase (GNMT) were significantly altered after GCRV infection...
April 7, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29626481/nicotine-induces-aberrant-hypermethylation-of-tumor-suppressor-genes-in-pancreatic-epithelial-ductal-cells
#5
Tong Jin, Jianyu Hao, Daiming Fan
Tobacco smoking is an independent risk factor for the initiation of pancreatic cancer (PC). Hypermethylation of tumor suppressor genes has been demonstrated to be associated with smoking. This study aimed to find the relationship between nicotine exposure and hypermethylation of tumor suppressor genes in normal pancreatic epithelial cells. Human pancreatic epithelial cells ware cultured exposing to nicotine and the methylation status of tumor suppressor genes were detected. Proenkephalin (PENK) was chosen as the target gene and methylation level of PENK promoter region was measured...
April 4, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29620185/procaine-stimulates-aquaporin%C3%A2-5-expression-in-human-salivary-gland-ductal-cells-via-the-suppression-of-dna-methyltransferase%C3%A2-1
#6
Fan Wu, Jintao Wang, Jianming Sun, Liman Shen, Meijuan Liu, Erjun Zhao
The present study aimed to investigate whether procaine may upregulate the expression of aquaporin‑5 (AQP5) in human salivary gland ductal cells and the underlying mechanisms of this upregulation. Immortalized normal human salivary gland ductal cells (NS‑SV‑DC), lacking AQP5 protein expression, were used to measure the glandular secretion rate following treatment with procaine, and the protein expression levels of AQP5 in NS‑SV‑DC cells were measured by western blotting. In order to investigate the mechanism of procaine action on AQP5 protein expression, the protein expression and activity of DNA methyltransferase (DNMT)1, and the CpG methylation of AQP5, were investigated further...
March 29, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29614648/altered-microrna-mrna-and-protein-expression-of-neurodegeneration-related-biomarkers-and-their-transcriptional-and-epigenetic-modifiers-in-a-human-tau-transgenic-mouse-model-in-response-to-developmental-lead-exposure
#7
Anwar M Masoud, Syed W Bihaqi, Bothaina Alansi, Miriam Dash, Gehad M Subaiea, William E Renehan, Nasser H Zawia
Amyloid deposits originating from the amyloid-β protein precursor (AβPP) and aggregates of the microtubule associated protein tau (MAPT) are the hallmarks of Alzheimer's disease (AD). Animal studies have demonstrated a link between early life exposure to lead (Pb) and latent overexpression of the AβPP and MAPT genes and their products via epigenetic reprogramming. The present study monitored APP gene and epigenetic mediators and transcription factors known to regulate it. Western blot analysis and quantitative polymerase chain reaction (qPCR) were used to study the mRNA, miRNA, and proteins levels of AβPP, specificity protein 1 (SP1; a transcriptional regulator of amyloid and tau pathway), and epigenetic intermediates namely: DNA methyltransferase (DNMT) 1, DNMT3a and Methyl- CpG protein binding 2 (MeCP2) in the cerebral cortex of transgenic mice (Knock-in for human MAPT)...
March 28, 2018: Journal of Alzheimer's Disease: JAD
https://www.readbyqxmd.com/read/29605852/genome-wide-profiling-of-dna-methyltransferases-in-mammalian-cells
#8
Massimiliano Manzo, Christina Ambrosi, Tuncay Baubec
Chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq) is currently the method of choice to determine binding sites of chromatin-associated factors in a genome-wide manner. Here, we describe a method to investigate the binding preferences of mammalian DNA methyltransferases (DNMT) based on ChIP-seq using biotin-tagging. Stringent ChIP of DNMT proteins based on the strong interaction between biotin and avidin circumvents limitations arising from low antibody specificity and ensures reproducible enrichment...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29603113/fucoxanthin-elicits-epigenetic-modifications-nrf2-activation-and-blocking-transformation-in-mouse-skin-jb6-p-cells
#9
Yuqing Yang, Irene Yang, Mingnan Cao, Zheng-Yuan Su, Renyi Wu, Yue Guo, Mingzhu Fang, Ah-Ng Kong
Nuclear factor erythroid-2-related factor-2 (Nrf2 or NFE2L2) is a master regulator of the anti-oxidative stress response, which is involved in the defense against many oxidative stress/inflammation-mediated diseases, including anticancer effects elicited by an increasing number of natural products. Our previous studies showed that the epigenetic modification of the Nrf2 gene plays a key role in restoring the expression of Nrf2. In this study, we aimed to investigate the epigenetic regulation of Nrf2 by astaxanthin (AST) and fucoxanthin (FX), carotenoids which are abundant in microalgae and seaweeds, in mouse skin epidermal JB6 P+ cells...
February 20, 2018: AAPS Journal
https://www.readbyqxmd.com/read/29601907/inhibition-of-dnmt-suppresses-the-stemness-of-colorectal-cancer-cells-through-down-regulating-wnt-signaling-pathway
#10
Shanxin Li, Zhipeng Han, Naping Zhao, Bing Zhu, Qianwen Zhang, Xue Yang, Dandan Sheng, Jing Hou, Shiwei Guo, Lixin Wei, Li Zhang
Cancer stem cell (CSC) theory reveals a new insight into the understanding of tumorigenesis and metastasis. Recently, DNA methylation is suggested to be a potential epigenetic mechanism for maintenance of CSCs. What's more, studies have shown that DNA methyltransferase (DNMT) is essential for CSCs and deletion of DNMT can reduce tumorigenesis by limiting CSC pool. Therefore, targeting the epigenetic modifiers especially DNA methylation offers an optional strategy for treating human cancers. In the present study we found that DNMT inhibitor 5-Aza-2'-deoxycytidine (5-AzaDC) markedly reduced colorectal CSC abundance in vitro and suppressed liver metastatic tumor growth in vivo...
March 27, 2018: Cellular Signalling
https://www.readbyqxmd.com/read/29578180/effect-of-lenalidomide-on-the-human-gastric-cancer-cell-line-sgc7901-vincristine-notch-signaling
#11
Wanjun Ding, Tao Zeng, Weiping Tao, Wei Ge, Junjian Deng, Hongbo Lei, Yong Xiao, Fei Liao
Aim of Study: To examine the function of lenalidomide (LEN) on the human multidrug resistance (MDR)-type gastric cancer line SGC7901/vincristine (VCR) via regulating Notch signaling. Materials and Methods: Quantitative polymerase chain reaction was used for checking the genes of Notch, DNA methyltransferase (DNMT), RBP-J, Hes1/5, Deltex1, MDR/multidrug resistant protein (MRP); the cell proliferation and cell death were detected by cell counting kit-8 (CCK8) staining, Ki-67 expression, and propidium-iodide staining, and methylated DNA immunoprecipitation assay (MeDIP) was used for checking the 5 mC enrichment, indicating the DNA methylation of the Notch2 gene loci...
2018: Journal of Cancer Research and Therapeutics
https://www.readbyqxmd.com/read/29568927/mir-185-and-mir-29a-are-similarly-expressed-in-the-bronchoalveolar-lavage-cells-in-ipf-and-lung-cancer-but-common-targets-dnmt1-and-col1a1-show-disease-specific-patterns
#12
Eleni Bibaki, Eliza Tsitoura, Eirini Vasarmidi, George Margaritopoulos, Athina Trachalaki, Chara Koutoulaki, Theodora Georgopoulou, Demetrios A Spandidos, Nikos Tzanakis, Katerina M Antoniou
Idiopathic pulmonary fibrosis (IPF) and lung cancer (LC) constitute two progressively devastating lung diseases with common risk factors including aging and smoking. There is an increasing interest in the investigation of common pathogenic mechanisms between IPF and LC with therapeutic implications. Several oncomirs, microRNAs associated with malignancy, are also linked with IPF. miR‑29a and miR‑185 downregulation is probably involved both in carcinogenesis and fibrogenesis. We have previously observed miR‑29a and miR‑185 downregulation in IPF cells from bronchoalveolar lavage (BAL) and in this study we investigated their expression in LC BAL cells...
March 19, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29560091/epigenetic-modifications-in-hyperhomocysteinemia-potential-role-in-diabetic-retinopathy-and-age-related-macular-degeneration
#13
Khaled Elmasry, Riyaz Mohamed, Isha Sharma, Nehal M Elsherbiny, Yutao Liu, Mohamed Al-Shabrawey, Amany Tawfik
To study Hyperhomocysteinemia (HHcy)-induced epigenetic modifications as potential mechanisms of blood retinal barrier (BRB) dysfunction, retinas isolated from three- week-old mice with elevated level of Homocysteine (Hcy) due to lack of the enzyme cystathionine β-synthase ( cbs-/- , cbs+/- and cbs+/+ ), human retinal endothelial cells (HRECs), and human retinal pigmented epithelial cells (ARPE-19) treated with or without Hcy were evaluated for (1) histone deacetylases (HDAC), (2) DNA methylation (DNMT), and (3) miRNA analysis...
February 27, 2018: Oncotarget
https://www.readbyqxmd.com/read/29555308/overexpression-of-dnmt1-leads-to-hypermethylation-of-h19-promoter-and-inhibition-of-erk-signaling-pathway-in-disuse-osteoporosis
#14
Bing Li, Jie Zhao, Jian-Xiong Ma, Guo-Min Li, Yang Zhang, Guo-Sheng Xing, Jun Liu, Xin-Long Ma
Disuse osteoporosis (DOP) is a common complication of the lack of mechanical loading. The precise mechanism underlying DOP remains unknown, although epigenetic modifications may be a major cause. Recently, cumulative research has revealed that DNA methyltransferase (DNMT) proteins can catalyze the conversion of cytosine to 5-methylcytosine (5mC), altering the epigenetic state of DNA. Here, we report that DNMT1 expression and lncRNA-H19 methylation are upregulated in the femoral tissues of DOP rats, accompanied with inhibited Erk signaling pathway...
March 16, 2018: Bone
https://www.readbyqxmd.com/read/29540744/alpha-oxoglutarate-inhibits-the-proliferation-of-immortalized-normal-bladder-epithelial-cells-via-an-epigenetic-switch-involving-arid1a
#15
Muhammad Shahid, Nicole Gull, Austin Yeon, Eunho Cho, Jooeun Bae, Hyun Seok Yoon, Sungyong You, Hana Yoon, Minjung Kim, Benjamin P Berman, Jayoung Kim
Interstitial cystitis (IC) is a chronic urinary tract disease that is characterized by unpleasant sensations, such as persistent pelvic pain, in the absence of infection or other identifiable causes. We previously performed comprehensive metabolomics profiling of urine samples from IC patients using nuclear magnetic resonance and gas-chromatography/mass spectrometry and found that urinary α-oxoglutarate (α-OG), was significantly elevated. α-OG, a tricarboxylic acid (TCA) cycle intermediate, reportedly functions to suppress the proliferation of immortalized normal human bladder epithelial cells...
March 14, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29535798/evaluation-of-potential-mechanisms-controlling-the-catalase-expression-in-breast-cancer-cells
#16
Christophe Glorieux, Juan Marcelo Sandoval, Nicolas Dejeans, Sandrine Nonckreman, Khadija Bahloula, Hélène A Poirel, Pedro Buc Calderon
Development of cancer cell resistance against prooxidant drugs limits its potential clinical use. MCF-7 breast cancer cells chronically exposed to ascorbate/menadione became resistant (Resox cells) by increasing mainly catalase activity. Since catalase appears as an anticancer target, the elucidation of mechanisms regulating its expression is an important issue. In MCF-7 and Resox cells, karyotype analysis showed that chromosome 11 is not altered compared to healthy mammary epithelial cells. The genomic gain of catalase locus observed in MCF-7 and Resox cells cannot explain the differential catalase expression...
2018: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/29528755/emerging-therapeutic-targets-for-the-treatment-of-malignant-rhabdoid-tumors
#17
Karolina Nemes, Michael C Frühwald
Malignant Rhabdoid Tumor (MRT) is a rare and highly aggressive malignancy primarily affecting infants and young children. The most common anatomic locations are the central nervous system (AT/RT), the kidneys (RTK) and other soft tissues (eMRT). The genetic origin of this disease is linked to mutations in SMARCB1, a gene encoding a core subunit of the SWI/SNF chromatin-remodeling complex. Areas covered: Conventional multimodal treatment may offer a significant survival benefit to certain patients. It remains to be determined, however, which patients will prove resistant to chemotherapy and need novel therapeutic approaches...
March 12, 2018: Expert Opinion on Therapeutic Targets
https://www.readbyqxmd.com/read/29525533/maternal-green-tea-polyphenol-intake-during-lactation-attenuates-kidney-injury-in-high-fat-diet-fed-male-offspring-programmed-by-maternal-protein-restriction-in-rats
#18
Saori Kataoka, Toshio Norikura, Shin Sato
Maternal malnutrition is known to increase the risk of obesity in offspring. We investigated whether green tea extract (GTE) intake during lactation affects obesity-related fibrosis and inflammation in the kidney of high-fat-diet-fed adult offspring of protein-restricted-diet-fed dams during pregnancy and lactation. Pregnant Wistar rats received diets containing 20% (normal-protein, NP) or 8% (low-protein, LP) casein, and they received 0%-, 0.12%- or 0.24%-GTE-containing LP diets (LP/LP, LP/LGT and LP/HGT, respectively) during lactation...
February 9, 2018: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/29525530/sulforaphane-epigenetically-demethylates-the-cpg-sites-of-the-mir-9-3-promoter-and-reactivates-mir-9-3-expression-in-human-lung-cancer-a549-cells
#19
Linbo Gao, David Cheng, Jie Yang, Renyi Wu, Wenji Li, Ah-Ng Kong
Increasing evidence suggests that epigenetic aberrations contribute to the development and progression of cancers such as lung cancer. The promoter region of miR-9-3 was recently found to be hypermethylated in lung cancer, resulting in down-regulation of miR-9-3 and poor patient prognosis. Sulforaphane (SFN), a natural compound that is obtained from cruciferous vegetables, has potent anticancer activities. In this study, we aimed to investigate the effect of SFN on restoring the miR-9-3 level in lung cancer A549 cells through epigenetic regulation...
February 9, 2018: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/29484388/increased-cytomegalovirus-replication-by-5-azacytidine-and-viral-induced-cytoplasmic-expression-of-dnmt%C3%A2-1-in-medulloblastoma-and-endothelial-cells
#20
Atosa Estekizadeh, Natalia Landázur, Jiri Bartek, Christian Beltoft Brøchner, Belghis Davoudi, Helle Broholm, Mohsen Karimi, Tomas J Ekström, Afsar Rahbar
Among all brain tumors diagnosed in children, medulloblastomas (MBs) are associated with a poor prognosis. The etiology of MB is not fully understood, yet the impact of epigenetic alterations of oncogenes has previously been established. During the past decade, the human cytomegalovirus (HCMV) has been detected in several types of cancer, including MB. Since DNA methylation occurs in the cell nucleus and this is considered a host defence response, we studied the impact of HCMV infection on DNA methyltransferase (DNMT‑1) in MB (D324) cells, human umbilical vein endothelial cells (HUVECs) as well as in MB tissue sections...
February 26, 2018: International Journal of Oncology
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