keyword
MENU ▼
Read by QxMD icon Read
search

Spermatogonia stem cell

keyword
https://www.readbyqxmd.com/read/28087628/id4-levels-dictate-the-stem-cell-state-in-mouse-spermatogonia
#1
Aileen R Helsel, Qi-En Yang, Melissa J Oatley, Tessa Lord, Fred Sablitzky, Jon M Oatley
Spermatogenesis is a classic model of cycling cell lineages that depend on a balance between stem cell self-renewal for continuity and formation of progenitors as the initial step in production of differentiated cells. However, the mechanisms guiding the continuum of spermatogonial stem cell (SSC) to progenitor spermatogonial transition and precise identifiers of subtypes in the process are undefined. Here we used an Id4-eGfp reporter mouse to discover that EGFP intensity is predictive of the subsets with the ID4-EGFP(Bright) population being mostly, if not purely, SSCs while the ID4-EGFP(Dim) population is in transition to the progenitor state...
January 13, 2017: Development
https://www.readbyqxmd.com/read/28077131/granulocyte-colony-stimulating-factor-g-csf-promotes-spermatogenic-regeneration-from-surviving-spermatogonia-after-high-dose-alkylating-chemotherapy
#2
Travis Kotzur, Roberto Benavides-Garcia, Jennifer Mecklenburg, Jamila R Sanchez, Matthew Reilly, Brian P Hermann
BACKGROUND: The lifesaving chemotherapy and radiation treatments that allow patients to survive cancer can also result in a lifetime of side-effects, including male infertility. Infertility in male cancer survivors is thought to primarily result from killing of the spermatogonial stem cells (SSCs) responsible for producing spermatozoa since SSCs turn over slowly and are thereby sensitive to antineoplastic therapies. We previously demonstrated that the cytokine granulocyte colony-stimulating factor (G-CSF) can preserve spermatogenesis after alkylating chemotherapy (busulfan)...
January 11, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/28071722/spict-a-cyst-cell-specific-gene-regulates-starvation-induced-spermatogonial-cell-death-in-the-drosophila-testis
#3
Ason C-Y Chiang, Heiko Yang, Yukiko M Yamashita
Tissues are maintained in a homeostatic state by balancing the constant loss of old cells with the continued production of new cells. Tissue homeostasis can shift between high and low turnover states to cope with environmental changes such as nutrient availability. Recently, we discovered that the elimination of transit-amplifying cells plays a critical role in maintaining the stem cell population during protein starvation in the Drosophila testis. Here, we identify spict, a gene expressed specifically in differentiating cyst cells, as a regulator of spermatogonial death...
January 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28053620/establishment-of-a-surgically-induced-cryptorchidism-canine-recipient-model-for-spermatogonial-stem-cell-transplantation
#4
Won-Young Lee, Ran Lee, Hyuk Song, Tai-Young Hur, Seunghoon Lee, Jiyun Ahn, Hyunjhung Jhun
Transplantation of spermatogonial stem cells (SSCs) in experimental animal models has been used to study germ line stem cell biology and to produce transgenic animals. The species-specific recipient model preparation is important for the characterization of SSCs and the production of offspring. Here, we investigated the effects of surgically induced cryptorchidism in dog as a new recipient model for spermatogonial stem cell transplantation. Artificially unilateral or bilateral cryptorchidism was induced in ten mature male dogs by surgically returning the testis and epididymis to the abdominal cavity...
December 2016: Laboratory Animal Research
https://www.readbyqxmd.com/read/28003832/identification-of-chd1l-as-an-important-regulator-for-spermatogonial-stem-cell-survival-and-self-renewal
#5
Shan-Shan Liu, Yin-Shan Bai, Li Feng, Wen-Wei Dong, Yang Li, Li-Ping Xu, Ning-Fang Ma
Chromodomain helicase/ATPase DNA binding protein 1-like gene (Chd1l) participates in chromatin-dependent processes, including transcriptional activation and DNA repair. In this study, we have found for the first time that Chd1l is mainly expressed in the testicular tissues of prepubertal and adult mice and colocalized with PLZF, OCT4, and GFRα1 in the neonatal mouse testis and THY1(+) undifferentiated spermatogonia or spermatogonial stem cells (SSCs). Knockdown of endogenous Chd1l in cultured mouse undifferentiated SSCs inhibited the expression levels of Oct4, Plzf, Gfrα1, and Pcna genes, suppressed SSC colony formation, and reduced BrdU incorporation, while increasing SSC apoptosis...
2016: Stem Cells International
https://www.readbyqxmd.com/read/27990704/differentiation-of-bone-marrow-derived-stage-specific-embryonic-antigen-1-positive-pluripotent-stem-cells-into-male-germ-cells
#6
Reza Shirazi, Amir Hassan Zarnani, Masoud Soleimani, Karim Nayernia, Iraj Ragerdi Kashani
Studies published in recent years have changed the outlook on sterility and germ cell development by producing gametes from stem cells. In present study, a novel approach on differentiation of bone marrow-derived stage-specific embryonic antigen 1 positive (SSEA-1(+) ) pluripotent stem cells into male germ cells has been addressed. SSEA-1(+) stem cells were separated from murine bone marrow using magnetic-activated cell sorting (MACS) system and propagated on a feeder layer cells. To evaluate the pluripotency characteristic of the purified cells, they were differentiated toward cells of three germ layers...
December 19, 2016: Microscopy Research and Technique
https://www.readbyqxmd.com/read/27927847/preserved-seminiferous-tubule-integrity-with-spermatogonial-survival-and-induction-of-sertoli-and-leydig-cell-maturation-after-long-term-organotypic-culture-of-prepubertal-human-testicular-tissue
#7
F de Michele, J Poels, L Weerens, C Petit, Z Evrard, J Ambroise, D Gruson, C Wyns
STUDY QUESTION: Is an organotypic culture system able to provide the appropriate testicular microenvironment for in-vitro maturation of human immature testicular tissue (ITT)? SUMMARY ANSWER: Our organotypic culture system provided a microenvironment capable of preserving seminiferous tubule (ST) integrity and Leydig cell (LC) functionality and inducing Sertoli cell (SC) maturation. WHAT IS KNOWN ALREADY: Cryopreservation of human ITT is a well-established strategy to preserve fertility in prepubertal boys affected by cancer, with a view for obtaining sperm...
January 2017: Human Reproduction
https://www.readbyqxmd.com/read/27926879/in%C3%A2-vitro-derivation-and-propagation-of-spermatogonial-stem-cell-activity-from-mouse-pluripotent-stem-cells
#8
Yukiko Ishikura, Yukihiro Yabuta, Hiroshi Ohta, Katsuhiko Hayashi, Tomonori Nakamura, Ikuhiro Okamoto, Takuya Yamamoto, Kazuki Kurimoto, Kenjiro Shirane, Hiroyuki Sasaki, Mitinori Saitou
The in vitro derivation and propagation of spermatogonial stem cells (SSCs) from pluripotent stem cells (PSCs) is a key goal in reproductive science. We show here that when aggregated with embryonic testicular somatic cells (reconstituted testes), primordial germ cell-like cells (PGCLCs) induced from mouse embryonic stem cells differentiate into spermatogonia-like cells in vitro and are expandable as cells that resemble germline stem cells (GSCs), a primary cell line with SSC activity. Remarkably, GSC-like cells (GSCLCs), but not PGCLCs, colonize adult testes and, albeit less effectively than GSCs, contribute to spermatogenesis and fertile offspring...
December 6, 2016: Cell Reports
https://www.readbyqxmd.com/read/27919445/effective-production-of-recipient-male-pigs-for-spermatogonial-stem-cell-transplantation-by-intratesticular-injection-with-busulfan
#9
Zhuoheng Lin, Jiajing Bao, Qunfang Kong, Yaofu Bai, Fenhua Luo, Zhou Songyang, Yingji Wu, Junjiu Huang
Germ cell transplantation has facilitated spermatogonial stem cell (SSC) and spermatogenesis research and shown great potential in the seed-breeding of domestic livestock. However, little progress has been made in large animals, primarily reflecting the difficulties in preparing sterile recipients. Here, we developed a novel protocol to prepare recipient pigs through the direct injection of busulfan into the cavum vaginale of the scrotums of Landrace-Large bi-crossbreeding male pigs and Seghers male pigs, two economically-important types of pigs, to eliminate endogenous spermatogonia...
February 2017: Theriogenology
https://www.readbyqxmd.com/read/27869554/genome-wide-deletion-screening-with-the-array-cgh-method-in-mouse-offspring-derived-from-irradiated-spermatogonia-indicates-that-mutagenic-responses-are-highly-variable-among-genes
#10
Jun-Ichi Asakawa, Mieko Kodaira, Akiko Miura, Takahiro Tsuji, Yoshiko Nakamoto, Masaaki Imanaka, Jun Kitamura, Harry Cullings, Mayumi Nishimura, Yoshiya Shimada, Nori Nakamura
Until the end of the 20th century, mouse germ cell data on induced mutation rates, which were collected using classical genetic methods at preselected specific loci, provided the principal basis for estimates of genetic risks from radiation in humans. The work reported on here is an extension of earlier efforts in this area using molecular methods. It focuses on validating the use of array comparative genomic hybridization (array CGH) methods for identifying radiation-induced copy number variants (CNVs) and specifically for DNA deletions...
December 2016: Radiation Research
https://www.readbyqxmd.com/read/27862128/regulation-of-crucial-lncrnas-in-differentiation-of-chicken-embryonic-stem-cells-to-spermatogonia-stem-cells
#11
D Li, Y Ji, F Wang, Y Wang, M Wang, C Zhang, W Zhang, Z Lu, C Sun, M F Ahmed, N He, K Jin, S Cheng, Y Wang, Y He, J Song, Y Zhang, B Li
Regulation of crucial lncRNAs involved in differentiation of chicken embryonic stem cells (ESCs) to spermatogonia stem cells (SSCs) was explored by sequencing the transcriptome of ESCs, primordial germ cells (PGCs) and SSCs with RNA-Seq; analytical bioinformatic methods were used to excavate candidate lncRNAs. We detected expression of candidate lncRNAs in ESCs, PGCs and SSCs and forecasted related target genes. Utilizing wego, david and string, function and protein-protein interactions of target genes were analyzed...
November 14, 2016: Animal Genetics
https://www.readbyqxmd.com/read/27861152/rad9a-is-required-for-spermatogonia-differentiation-in-mice
#12
Lin Huang, Zhen-Bo Wang, Shu-Tao Qi, Xue-Shan Ma, Qiu-Xia Liang, Guo Lei, Tie-Gang Meng, Li-Feng Liang, Ye-Xin Xian, Yi Hou, Xiao-Fang Sun, Yong Zhao, Wei-Hua Wang, Qing-Yuan Sun
Spermatogenesis in testes requires precise spermatogonia differentiation. Spermatocytes lacking the Rad9a gene are arrested in pachytene prophase, implying a possible role for RAD9A in spermatogonia differentiation. However, numerous RAD9A-positive pachytene spermatocytes are still observed in mouse testes following Rad9a excision using the Stra8-Cre system, and it is unclear whether Rad9a deletion in spermatogonia interrupts differentiation. Here, we generated a mouse model in which Rad9a was specifically deleted in spermatogonial stem cells (SSCs) using Cre recombinase expression driven by the germ cell-specific Vasa promoter...
November 16, 2016: Oncotarget
https://www.readbyqxmd.com/read/27834666/proliferation-in-culture-of-primordial-germ-cells-derived-from-embryonic-stem-cell-induction-by-retinoic-acid
#13
Zohreh Makoolati, Mansoureh Movahedin, Mehdi Forouzandeh-Moghadam
An in vitro system that supports primordial germ cells (PGCs) survival and proliferation is useful for enhancement of these cells and efficient transplantation in infertility disorders. One approach is cultivation of PGCs under proper conditions that allow self-renewal and proliferation of PGCs. For this purpose, we compared the effects of different concentrations of retinoic acid (RA), and the effect of PGCs co-culture (Co-C) with SIM mouse embryo-derived thioguanine- and ouabain-resistant (STO) cells on the proliferation of embryonic stem cells (ESCs)-derived PGCs...
December 2016: Bioscience Reports
https://www.readbyqxmd.com/read/27827452/gdnf-expressing-sto-feeder-layer-supports-the-long-term-propagation-of-undifferentiated-mouse-spermatogonia-with-stem-cell-properties
#14
Xiang Wei, Yuanyuan Jia, Yuanyuan Xue, Lei Geng, Min Wang, Lufan Li, Mei Wang, Xuemei Zhang, Xin Wu
The development of a stem cell culture system would expedite our understanding of the biology of tissue regeneration. Spermatogonial stem cell (SSC) is the foundation for lifelong male spermatogenesis and the SSC culture has been optimized continuously in recent years. However, there have been many inconveniences to reconstruct SSC self-renewal and proliferation in vitro, such as the frequent refreshment of recombinant cytokines, including GDNF, the essential growth factor for SSC maintenance. In the present study, we observed that both STO and MEF cells, which were previously used as feeders for SSC growth, did not express GDNF, but a GDNF-expressing STO feeder could support undifferentiated mouse spermatogonia propagation in vitro for three months without the refreshment of recombinant growth factor GDNF...
November 9, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27822884/lncrna-ak015322-promotes-proliferation-of-spermatogonial-stem-cell-c18-4-by-acting-as-a-decoy-for-microrna-19b-3p
#15
Ke Hu, Jing Zhang, Meng Liang
Long noncoding RNAs (lncRNAs) have been reported to play important roles in male reproduction. In our previous research, we studied the expression profile of lncRNAs in mouse male germ cells including spermatogonial stem cell, type A spermatogonia, pachytene spermatocyte, and round spermatid by microarray method, which showed that testis-enriched lncRNA AK015322 is highly expressed in spermatogonial stem cell. In this study, we found that AK015322 promotes proliferation of mouse spermatogonial stem cell line C18-4 in vitro...
November 7, 2016: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/27821019/molecular-signatures-of-primary-human-spermatogonial-progenitors-and-its-neighboring-peritubular-stromal-compartment
#16
Abhishek Harichandan, Kavitha Sivasubramaniyan, Jörg Hennenlotter, Sven Poths, Jens Bedke, Stephan Kruck, Arnulf Stenzl, Hans-Jörg Bühring
In-depth understanding of human spermatogenesis requires studying specific molecular signatures and interactions of spermatogonia with other testicular cell populations, for which isolation of pure populations of different cell types is crucial. Here, we describe a technique to simultaneously enrich pure, multiple testicular cell populations, including spermatogonia, endothelial (TECs), and perivascular mesenchymal stem/stromal cells (TMSCs), from testicular tissue by flow cytometry using a combination of defined markers...
December 16, 2016: Stem Cells and Development
https://www.readbyqxmd.com/read/27819720/male-idiopathic-infertility-and-the-tp53-polymorphism-in-codon-72
#17
M P de Morais, R F Curado, K S F E Silva, K K V O Moura, J T Arruda
Many environmental agents affect the development of male germ cells at different stages. Apoptosis is common during normal spermatogenesis; it plays an important role in controlling the number of germ cells and the disposal of defective stem cells to produce functional sperm. The presence of p53 in primary spermatocytes suggests that it plays a role in the prophase of meiosis. p53 is expressed in the testis in both spermatocytes and spermatogonia. This suggests that the p53 gene (TP53) is important for apoptosis regulation during spermatogenesis, and may be associated with male infertility...
November 3, 2016: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/27798668/the-lin28-expression-in-stallion-testes
#18
Geumhui Lee, Heejun Jung, Minjung Yoon
The molecular markers for specific germ cell stages can be utilized for identifying, monitoring, and separating a particular stage of germ cells. The RNA-binding protein Lin28 is expressed in gonocytes of human fetal testes. The Lin28 expression is restricted to a very small population of spermatogonial cells in human, mice, and monkey. The main objective of this study was to investigate the expression pattern of Lin28 in stallion testes at different reproductive stages. Based on the presence or absence of full spermatogenesis and lumina in seminiferous tubules, the testicular samples were categorized into two reproductive stages pre-pubertal and post-pubertal...
2016: PloS One
https://www.readbyqxmd.com/read/27795714/bmp4-cooperates-with-retinoic-acid-to-induce-the-expression-of-differentiation-markers-in-cultured-mouse-spermatogonia
#19
Yongguang Yang, Yanmin Feng, Xue Feng, Shangying Liao, Xiuxia Wang, Haiyun Gan, Lixian Wang, Xiwen Lin, Chunsheng Han
Spermatogenesis is sustained by the proliferation and differentiation of spermatogonial stem cells (SSCs). However, the molecules controlling these processes remain largely unknown. Here, we developed a simplified high concentration serum-containing system for the culture of mouse SSCs. Analysis of SSCs markers and transplantation results revealed that the cultured spermatogonia retained stem cell characteristics after long-term in vitro propagation. Using this culture system, the expression and function of bone morphogenetic protein 4 (BMP4) were explored...
2016: Stem Cells International
https://www.readbyqxmd.com/read/27774720/immunolocalization-of-proteins-in-the-spermatogenesis-process-of-canine
#20
Ncg Pieri, A F Souza, Acf Mançanares, Kcs Roballo, J B Casals, C E Ambrosio, D S Martins
Spermatogenesis is a process in which differentiated cells are produced and the adult stem cell population-known as spermatogonial stem cells (SSCs)-is continuously replenished. However, the molecular mechanisms underlying these processes are not fully understood in the canine species. We addressed this in this study by analysing the expression of specific markers in spermatogonia of seminiferous tubules of canine testes. SSCs at different stages of reproductive development (prepubertal and adult) were examined by immunohistochemistry and flow cytometry...
October 23, 2016: Reproduction in Domestic Animals, Zuchthygiene
keyword
keyword
32048
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"