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Spermatogonia stem cell

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https://www.readbyqxmd.com/read/28542371/whole-genome-sequencing-of-spermatocytic-tumors-provides-insights-into-the-mutational-processes-operating-in-the-male-germline
#1
Eleni Giannoulatou, Geoffrey J Maher, Zhihao Ding, Ad J M Gillis, Lambert C J Dorssers, Alexander Hoischen, Ewa Rajpert-De Meyts, Gilean McVean, Andrew O M Wilkie, Leendert H J Looijenga, Anne Goriely
Adult male germline stem cells (spermatogonia) proliferate by mitosis and, after puberty, generate spermatocytes that undertake meiosis to produce haploid spermatozoa. Germ cells are under evolutionary constraint to curtail mutations and maintain genome integrity. Despite constant turnover, spermatogonia very rarely form tumors, so-called spermatocytic tumors (SpT). In line with the previous identification of FGFR3 and HRAS selfish mutations in a subset of cases, candidate gene screening of 29 SpTs identified an oncogenic NRAS mutation in two cases...
2017: PloS One
https://www.readbyqxmd.com/read/28532845/characterization-of-undifferentiated-spermatogonia-and-the-spermatogonial-niche-in-the-lambari-fish-astyanax-altiparanae
#2
Marília de Paiva Camargo, Mônica Cassel, Lázaro Wender Oliveira de Jesus, Rafael Henrique Nóbrega, Maria Inês Borella
Undifferentiated type A spermatogonia are the foundation of fish spermatogenesis. This cell population includes the spermatogonial stem cell population, which is able to either self-renew or differentiate into cells that will generate the male gamete - the spermatozoa. Spermatogonia stem cells are located in a specific region of the testes known as the spermatogonial niche, which regulates spermatogonial stem cell function. This study characterizes undifferentiated type A spermatogonia and their S-phase label-retaining cell properties in Astyanax altiparanae testes...
July 1, 2017: Theriogenology
https://www.readbyqxmd.com/read/28531200/discovery-of-micrornas-during-early-spermatogenesis-in-chicken
#3
Lu Xu, Qixin Guo, Guobin Chang, Lingling Qiu, Xiangping Liu, Yulin Bi, Yu Zhang, Hongzhi Wang, Wei Lu, Lichen Ren, Ying Chen, Yang Zhang, Qi Xu, Guohong Chen
Spermatogenesis is a complex process that involves many elements. However, until now, little is known at the molecular level about spermatogenesis in poultry. Here we investigated microRNAs and their target genes that may be involved in germ cell development and spermatogonial in chicken. We used next-generation sequencing to analyze miRNA expression profiles in three types of germline cells: primordial germ cells (PGCs), spermatogonial stem cells (SSCs), and spermatogonia (Sp) during early stage of spermatogenesis...
2017: PloS One
https://www.readbyqxmd.com/read/28499702/prespermatogenesis-and-early-spermatogenesis-in-frogs
#4
Katarzyna Haczkiewicz, Beata Rozenblut-Kościsty, Maria Ogielska
Spermatogenesis in frogs was for the first time divided into two phases: prespermatogenesis, when gonocytes proliferate in developing tadpole testes, and active spermatogenesis when spermatogonial stem cells (i.e. descendants of gonocytes), either self-renew or enter into meiotic cycles within cysts formed by Sertoli cells. We argue that amphibian larval gonocytes are homologues to mammalian gonocytes, whereas spermatogonial stem cells (SSCs) in adult frogs are homologous to mammalian single spermatogonia (As)...
March 12, 2017: Zoology: Analysis of Complex Systems, ZACS
https://www.readbyqxmd.com/read/28498828/kdm1a-lsd1-regulates-the-differentiation-and-maintenance-of-spermatogonia-in-mice
#5
Dexter A Myrick, Michael A Christopher, Alyssa M Scott, Ashley K Simon, Paul G Donlin-Asp, William G Kelly, David J Katz
The proper regulation of spermatogenesis is crucial to ensure the continued production of sperm and fertility. Here, we investigated the function of the H3K4me2 demethylase KDM1A/LSD1 during spermatogenesis in developing and adult mice. Conditional deletion of Kdm1a in the testis just prior to birth leads to fewer spermatogonia and germ cell loss before 3 weeks of age. These results demonstrate that KDM1A is required for spermatogonial differentiation, as well as germ cell survival, in the developing testis...
2017: PloS One
https://www.readbyqxmd.com/read/28483168/hedgehog-gli1-signaling-regelates-differentiation-of-chicken-gallus-gallus-embryonic-stem-cells-to-male-germ-cells
#6
Dong Li, Shaoze Cheng, Wenhui Zhang, Man Wang, Changhua Sun, Chen Zhang, Yilin Wang, Jing Jin, Yani Zhang, Bichun Li
Gli1 is an important signaling molecular in Hedgehog signaling pathway. In our study, we explored the adjustment effect of Hedgehog-Gli1 signaling pathway on chicken male germ cells differentiation based on the transcriptome-wide analyses of chicken ESCs, primordial germ cells (PGCs) and spermatogonia stem cells (SSCs) that were associated with male germ cell differentiation. We screened out Hedgehog signaling pathway and identified 8 candidated differentially expressed genes (DEGs), Wnt3a, Wnt16, Wnt8a, HHIPL1, Gli1, BMP6, BMP7 and TTLL4...
February 10, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28481028/preservation-of-chromosomal-integrity-in-murine-spermatozoa-derived-from-gonocytes-and-spermatogonial-stem-cells-surviving-prenatal-and-postnatal-exposure-to-%C3%AE-rays-in-mice
#7
Hiroyuki Watanabe, Atsushi Kohda, Jun-Ichiro Komura, Hiroyuki Tateno
Pre- and postnatal male mice were acutely (659 - 690 mGy/min) and continuously (0.303 mGy/min) exposed to 2 Gy γ-rays to evaluate spermatogenic potential and chromosome damage in their germ cells as adults. Acute irradiation on Days 15.5, 16.5, and 17.5 post-coitus affected testicular development, as a result of massive quiescent gonocyte loss; the majority of the seminiferous tubules in these testes were devoid of germ cells. Acute irradiation on Days 18.5 and 19.5 post-coitus had less effect on testicular development and spermatogenesis, even though germ cells were quiescent gonocytes on these days...
May 8, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/28474535/long-term-propagation-of-porcine-undifferentiated-spermatogonia
#8
Pengfei Zhang, Xiaoxu Chen, Yi Zheng, Jinsheng Zhu, Yuwei Qin, Yinghua Lv, Wenxian Zeng
Spermatogonial stem cells (SSCs) provide the foundation for spermatogenesis and fertility throughout the adult life of a male. Genetic manipulations of SSCs combined with germ cell transplantation present a novel approach for gene therapy and production of genetically modified animals. However, the rarity of SSCs within mammalian testes remains an impediment to related applications that require sufficient numbers of SSCs, making in vitro expansion of SSCs a prerequisite. Nevertheless, long-term culture systems of SSCs from large animals have not been established yet...
May 5, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28465081/characterization-of-male-germ-cell-markers-in-canine-testis
#9
Won-Young Lee, Ran Lee, Hyun-Jung Park, Jeong Tae Do, Chankyu Park, Jin-Hoi Kim, Hyunjhung Jhun, Ji-Heon Lee, Taiyoung Hur, Hyuk Song
Spermatogenesis begins at puberty and continues throughout a male's life. This process is initiated and maintained by spermatogonial stem cells in the seminiferous tubules, and these cells produce haploid spermatozoa. Markers of male germ cells have been fully identified in rodents, including mice and rats, but not in canines. To characterize the canine male germ cells, histological and immunohistochemical analyses were performed, using prepubertal (1-3-month-old), early pubertal (4-month-old), and postpubertal (7-month-old) dog testes...
January 12, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28464323/ssm1b-expression-and-function-in-germ-cells-of-adult-mice-and-in-early-embryos
#10
Sarayu Ratnam, Grazyna Bozek, Christopher Payne, Shannon Gallagher, Terence Martin, Ursula Storb
Strain-specific modifier of DNA methylation 1b (Ssm1b) is a Krüppel-associated box (KRAB) zinc finger gene that promotes CpG methylation in the mouse transgene HRD (Heavy chain enhancer, Rearrangement by Deletion). Ssm1b expression and concomitant HRD methylation are also present in the male and female germ cells of adult mice. Ssm1b is expressed in both diploid (2N) and haploid (1N) oocytes, as well as in 1N spermatids and spermatozoa, but not in 2N spermatogonia. Interestingly, Ssm1b mRNA is not detected in any other adult mouse organ examined, although Ssm1-family mRNAs are highly expressed in the heart...
May 2, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/28461596/a-transit-amplifying-population-underpins-the-efficient-regenerative-capacity-of-the-testis
#11
Claudia Carrieri, Stefano Comazzetto, Amit Grover, Marcos Morgan, Andreas Buness, Claus Nerlov, Dónal O'Carroll
The spermatogonial stem cell (SSC) that supports spermatogenesis throughout adult life resides within the GFRα1-expressing A type undifferentiated spermatogonia. The decision to commit to spermatogenic differentiation coincides with the loss of GFRα1 and reciprocal gain of Ngn3 (Neurog3) expression. Through the analysis of the piRNA factor Miwi2 (Piwil4), we identify a novel population of Ngn3-expressing spermatogonia that are essential for efficient testicular regeneration after injury. Depletion of Miwi2-expressing cells results in a transient impact on testicular homeostasis, with this population behaving strictly as transit amplifying cells under homeostatic conditions...
May 1, 2017: Journal of Experimental Medicine
https://www.readbyqxmd.com/read/28456466/constitutive-wnt-ctnnb1-activation-triggers-spermatogonial-stem-cell-proliferation-and-germ-cell-depletion
#12
Anne-Amandine Chassot, Morgane Le Rolle, Maxime Jourden, Maketo M Taketo, Norbert B Ghyselinck, Marie-Christine Chaboissier
The differentiation of germ cells into oogonia or spermatogonia is the first step that eventually gives rise to fully mature gametes. In the female fetal gonad, the RSPO1/WNT/CTNNB1 signalling pathway is involved in primordial germ cell proliferation and differentiation into female germ cells, which are able to enter meiosis. In the postnatal testis, the WNT/CTNNB1 pathway also mediates proliferation of spermatogonial stem cells and progenitor cells. Here we show that forced activation of the WNT/CTNNB1 pathway in fetal gonocytes using transgenic mice leads to deregulated spermatogonial proliferation, and exhaustion of the spermatocytes by apoptosis, resulting in a hypoplastic testis...
April 26, 2017: Developmental Biology
https://www.readbyqxmd.com/read/28427670/estimation-of-the-distribution-of-low-intensity-ultrasound-mechanical-index-as-a-parameter-affecting-the-proliferation-of-spermatogonia-stem-cells-in-vitro
#13
Zeinab Hormozi Moghaddam, Manijhe Mokhtari-Dizaji, Mansoureh Movahedin, Mohammad Ehsan Ravari
Considering the use of physical and mechanical stimulation, such as low-intensity ultrasound for proliferation and differentiation of stem cells, it is essential to understand the physical and acoustical mechanisms of acoustic waves in vitro. Mechanical index is used for quantifying acoustic cavitation and the relationship between acoustic pressure and the frequency. In this study, modeling of the mechanical index was applied to provide treatment protocol and to understand the effective physical processes on reproducibility of stem cells...
July 2017: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/28408662/the-gdnf-responsive-phosphoprotein-landscape-identifies-raptor-phosphorylation-required-for-spermatogonial-progenitor-cell-proliferation
#14
Min Wang, Yueshuai Guo, Mei Wang, Tao Zhou, Yuanyuan Xue, Guihua Du, Xiang Wei, Jing Wang, Lin Qi, Hao Zhang, Lufan Li, Lan Ye, Xuejiang Guo, Xin Wu
Cytokine-dependent renewal of stem cells is a fundamental requisite for tissue homeostasis and regeneration. Spermatogonial progenitor cells (SPCs) including stem cells support life-long spermatogenesis and male fertility, but pivotal phosphorylation events that regulate fate decisions in SPCs remain unresolved. Here, we described a quantitative mass-spectrometry-based proteomic and phosphoproteomic analyses of SPCs following sustained stimulation with glial cell-derived neurotrophic factor (GDNF), an extrinsic factor supporting SPC proliferation...
April 13, 2017: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/28402059/molecular-markers-of-putative-spermatogonial-stem-cells-in-the-domestic-cat
#15
S J Bedford-Guaus, S Kim, L Mulero, J M Vaquero, C Morera, R Adan-Milanès, A Veiga, Á Raya
Spermatogonial stem cells (SSCs) are an important tool for fertility preservation and species conservation. The ability to expand SSCs by in vitro culture is a crucial premise for their use in assisted reproduction. Because SSCs represent a small proportion of the germ cells in the adult testis, culture success is aided by pre-enrichment through sorting techniques based on cell surface-specific markers. Given the importance of the domestic cat as a model for conservation of endangered wild felids, herein we sought to examine culture conditions as well as molecular markers for cat SSCs...
April 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28379293/cell-autonomous-requirement-for-mammalian-target-of-rapamycin-mtor-in-spermatogonial-proliferation-and-differentiation-in-the-mouse%C3%A2
#16
Nicholas D Serra, Ellen K Velte, Bryan A Niedenberger, Oleksander Kirsanov, Christopher B Geyer
Spermatogonial stem cells must balance self-renewal with production of transit-amplifying progenitors that differentiate in response to retinoic acid (RA) before entering meiosis. This self-renewal vs. differentiation fate decision is critical for maintaining tissue homeostasis, as imbalances cause defects that can lead to human testicular cancer or infertility. Little is currently known about the program of differentiation initiated by RA, and the pathways and proteins involved are poorly defined. We recently found that RA stimulation of the Phosphatidylinositol 3-kinase (PI3K)/AKT/Mammalian target of rapamycin (mTOR) kinase signaling pathway is required for differentiation, and that short-term inhibition of mTOR complex 1 (mTORC1) by rapamycin blocked spermatogonial differentiation in vivo and prevented RA-induced translational activation...
April 1, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/28373495/spermatogonial-stem-cell-transplantation-to-the-testis
#17
Shaun Goodyear, Ralph Brinster
Spermatogonial stem cells (SSCs) are located in the basal region of the seminiferous tubules and provide a self-renewing reservoir from which progenitor spermatogonia arise and transiently amplify in number before transition to a differentiating state. SSC transplantation involves grafting mouse testicular cells into the seminiferous tubules of an aspermic recipient testis that is generated either by chemical (e.g., busulfan) treatment or genetic mutations.
April 3, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28369535/the-production-of-glial-cell-line-derived-neurotrophic-factor-by-human-sertoli-cells-is-substantially-reduced-in-sertoli-cell-only-testes
#18
D Singh, D A Paduch, P N Schlegel, K E Orwig, A Mielnik, A Bolyakov, W W Wright
STUDY QUESTION: Do human Sertoli cells in testes that exhibit the Sertoli cell-only (SCO) phenotype produce substantially less glial cell line-derived neurotrophic factor (GDNF) than Sertoli cells in normal testes? SUMMARY ANSWER: In human SCO testes, both the amounts of GDNF mRNA per testis and the concentration of GDNF protein per Sertoli cell are markedly reduced as compared to normal testes. WHAT IS KNOWN ALREADY: In vivo, GDNF is required to sustain the numbers and function of mouse spermatogonial stem cells (SSCs) and their immediate progeny, transit-amplifying progenitor spermatogonia...
May 1, 2017: Human Reproduction
https://www.readbyqxmd.com/read/28289609/the-regenerative-effect-of-bone-marrow-derived-stem-cells-in-spermatogenesis-of-infertile-hamster
#19
Akbar Vahdati, Alireza Fathi, Mehrdokht Hajihoseini, Ghaem Aliborzi, Ebrahim Hosseini
BACKGROUND: Infertility is a serious social problem in advanced nations, with male factor in half of all cases of infertility. This study was conducted to determine the regenerative effect of bone marrow-derived stem cells in spermatogenesis of infertile hamster. METHODS: Twelve adult male hamsters were equally divided into azoospermic and control groups. Busulfan was intraperitoneally used for induction of azoospermia, while the right testis was treated with bone marrow-derived stem cells (10(6) BM-SCs), labeled with sterile trypan blue, 35 days after busulfan injection...
January 2017: World Journal of Plastic Surgery
https://www.readbyqxmd.com/read/28258155/doxorubicin-and-vincristine-affect-undifferentiated-rat-spermatogonia
#20
Hermance Beaud, Ans M M van Pelt, Géraldine Delbes
Anticancer drugs such as alkylating agents, can affect male fertility by targeting the DNA of proliferative spermatogonial stem cells (SSC). Therefore, to reduce such side effects, other chemotherapeutics are used. But less is known about their potential genotoxicity on SSC. Moreover, DNA repair mechanisms in SSC are poorly understood. To model treatments deprived of alkylating agents that are commonly used in cancer treatment, we tested the impact of exposure to doxorubicin and vincristine, alone or in combination (MIX), on a rat spermatogonial cell line with SSC characteristics (GC-6spg)...
March 3, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
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