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carboxylesterase

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https://www.readbyqxmd.com/read/28077333/molecular-cloning-characterization-and-expression-analysis-of-two-juvenile-hormone-esterase-like-carboxylesterase-cdnas-in-chinese-mitten-crab-eriocheir-sinensis
#1
Yu Xu, Muzi Zhao, Yanfei Deng, Yuanjie Yang, Xuguang Li, Quanping Lu, Jiachun Ge, Jianlin Pan, Zhiqiang Xu
Precise regulation of methyl farnesoate (MF) titer is of prime importance throughout the crustacean life-cycle. Although the synthetic pathway of MF is well-documented, little is known about its degradation and recycling in crustaceans. Juvenile hormone esterase-like (JHE-like) carboxylesterase (CXE) is a key enzyme in MF degradation, thus playing a significant role in regulating the MF titer. We identified and characterized two cDNAs, Es-CXE1 and Es-CXE2, encoding JHE-like CXEs in Chinese mitten crab. Full-length cDNAs of Es-CXE1 and Es-CXE2 encode proteins composed of 584 and 597 amino acids, respectively, both of which contain a typical carboxylesterase domain...
January 8, 2017: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/28067292/design-of-a-15-n-molecular-unit-to-achieve-long-retention-of-hyperpolarized-spin-state
#2
Hiroshi Nonaka, Masashi Hirano, Yuki Imakura, Yoichi Takakusagi, Kazuhiro Ichikawa, Shinsuke Sando
Nuclear hyperpolarization is a phenomenon that can be used to improve the sensitivity of magnetic resonance molecular sensors. However, such sensors typically suffer from short hyperpolarization lifetime. Herein we report that [(15)N, D14]trimethylphenylammonium (TMPA) has a remarkably long spin-lattice relaxation time (1128 s, 14.1 T, 30 °C, D2O) on its (15)N nuclei and achieves a long retention of the hyperpolarized state. [(15)N, D14]TMPA-based hyperpolarized sensor for carboxylesterase allowed the highly sensitive analysis of enzymatic reaction by (15)N NMR for over 40 min in phophate-buffered saline (H2O, pH 7...
January 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28058522/identification-and-characterization-of-a-novel-carboxylesterase-fpbh-that-hydrolyzes-aryloxyphenoxypropionate-herbicides
#3
Chenghong Wang, Jiguo Qiu, Youjian Yang, Jinwei Zheng, Jian He, Shunpeng Li
OBJECTIVE: To identify and characterize a novel aryloxyphenoxypropionate (AOPP) herbicide-hydrolyzing carboxylesterase from Aquamicrobium sp. FPB-1. RESULTS: A carboxylesterase gene, fpbH, was cloned from Aquamicrobium sp. FPB-1. The gene is 798 bp long and encodes a protein of 265 amino acids. FpbH is smaller than previously reported AOPP herbicide-hydrolyzing carboxylesterases and shares only 21-35% sequence identity with them. FpbH was expressed in Escherichia coli BL21(DE3) and the product was purified by Ni-NTA affinity chromatography...
January 5, 2017: Biotechnology Letters
https://www.readbyqxmd.com/read/28046150/increased-expressions-of-genes-and-proteins-involved-in-mitochondrial-oxidation-and-antioxidant-pathway-in-adipose-tissue-of-pigs-selected-for-a-low-residual-feed-intake
#4
I Louveau, A Vincent, S Tacher, H Gilbert, F Gondret
Adipose tissue is a primary sensor for nutrient availability and regulates many functions including feed intake and energy homeostasis. This study was undertaken to determine the molecular responses of adipose tissue to differences in feed intake and feed efficiency. Subcutaneous adipose tissue was collected from two lines of pigs divergently selected for residual feed intake (RFI), a measure of feed efficiency defined as the difference between actual and expected feed intake, and from a subset of high-RFI pigs that were feed-restricted at the level of the voluntary feed intake of low-RFI pigs during the growing-finishing period...
December 2016: Journal of Animal Science
https://www.readbyqxmd.com/read/28044227/a-novel-versatile-family-iv-carboxylesterase-exhibits-high-stability-and-activity-in-a-broad-ph-spectrum
#5
Amélie Dukunde, Dominik Schneider, Mingji Lu, Silja Brady, Rolf Daniel
OBJECTIVES: To investigate the properties of a novel metagenome-derived member of the hormone-sensitive lipase family of lipolytic enzymes. RESULTS: A forest soil metagenome-derived gene encoding an esterase (Est06) belonging to the hormone-sensitive lipase family of lipolytic enzymes was subcloned, heterologously expressed and characterized. Est06 is a polypeptide of 295 amino acids with a molecular mass of 31 kDa. The deduced protein sequence shares 61% similarity with a hypothetical protein from the marine symbiont Candidatus Entotheonella sp...
January 2, 2017: Biotechnology Letters
https://www.readbyqxmd.com/read/28007454/comparative-study-of-hydrolytic-metabolism-of-dimethyl-phthalate-dibutyl-phthalate-and-di-2-ethylhexyl-phthalate-by-microsomes-of-various-rat-tissues
#6
Hitomi Ozaki, Kazumi Sugihara, Yoko Watanabe, Kyoko Moriguchi, Naoto Uramaru, Tomomichi Sone, Shigeru Ohta, Shigeyuki Kitamura
Phthalates are used in food packaging, and are transferred to foods as contaminants. In this study, we examined the hydrolytic metabolism of dimethyl phthalate (DMP), dibutyl phthalate (DBP) and di(2-ethylhexyl) phthalate (DEHP) by rat tissue microsomes. We found that carboxylesterase and lipase contribute differently to these activities. When DMP, DBP and DEHP were incubated with rat liver microsomes, DBP was most effectively hydrolyzed to the phthalate monoester, followed by DMP, and the activity toward DEHP was marginal...
December 19, 2016: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/27998705/in%C3%A2-vitro-in%C3%A2-vivo-extrapolation-of-intestinal-availability-for-carboxylesterase-substrates-using-portal-vein-cannulated-monkey
#7
Patrick E Trapa, Kevin Beaumont, Karen Atkinson, Heather Eng, Amanda King-Ahmad, Dennis O Scott, Tristan S Maurer, Li Di
Prediction of intestinal availability (FaFg) of carboxylesterase (CES) substrates is of critical importance in designing oral prodrugs with optimal properties, projecting human pharmacokinetics and dose, and estimating drug-drug interaction potentials. A set of ester prodrugs were evaluated using in vitro permeability (parallel artificial membrane permeability assay and Madin-Darby canine kidney cell line-low efflux) and intestinal stability (intestine S9) assays, as well as in vivo portal vein-cannulated cynomolgus monkey...
December 18, 2016: Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/27986599/development-of-a-novel-formulation-that-improves-pre-clinical-bioavailability-of-tenofovir-disoproxil-fumarate
#8
M E Watkins, S Wring, R Randolph, S Park, K Powell, M Lutz, M Nowakowski, R Ramabhadran, P L Domanico
Tenofovir disoproxil fumarate (TDF), the bisphosphonate ester prodrug of tenofovir (TFV), has poor bioavailability due to intestinal degradation and efflux transport. Reformulation using FDA-approved esterase and efflux inhibitors to increase oral bioavailability could provide lower dose alternatives and reduce costs for patients with HIV in resource-limited settings (RLS). Inhibition of mucosal and intracellular esterases was studied in human and rat intestinal extracts (S9), where TDF was protected by the carboxylesterase (CE) inhibitor bis-para-nitrophenylphosphate (BNPP), the ester mix EM1, and the generally-recognized-as-safe (GRAS) excipient propylparaben (PP)...
December 13, 2016: Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/27936192/insecticidal-activity-of-melaleuca-alternifolia-essential-oil-and-rna-seq-analysis-of-sitophilus-zeamais-transcriptome-in-response-to-oil-fumigation
#9
Min Liao, Jin-Jing Xiao, Li-Jun Zhou, Yang Liu, Xiang-Wei Wu, Ri-Mao Hua, Gui-Rong Wang, Hai-Qun Cao
BACKGROUND: The cereal weevil, Sitophilus zeamais is one of the most destructive pests of stored cereals worldwide. Frequent use of fumigants for managing stored-product insects has led to the development of resistance in insects. Essential oils from aromatic plants including the tea oil plant, Melaleuca alternifolia may provide environmentally friendly alternatives to currently used pest control agents. However, little is known about molecular events involved in stored-product insects in response to plant essential oil fumigation...
2016: PloS One
https://www.readbyqxmd.com/read/27925273/biochemical-response-of-the-africanized-honeybee-exposed-to-fipronil
#10
Thaisa C Roat, Stephan M Carvalho, Mário S Palma, Osmar Malaspina
Bees are recognized worldwide for their social, economic, and environmental value; however, they have been seriously threatened in recent decades by diseases and by high levels of pesticide use. The susceptibility of bees to insecticides makes them an important terrestrial model for assessing environmental quality, and various biomarkers have been developed for these assessments. The present study aimed to evaluate the activity of the enzymes acetylcholinesterase (AChE), carboxylesterase (CaE), and glutathione S-transferase (GST) in Africanized honeybees exposed to fipronil...
December 7, 2016: Environmental Toxicology and Chemistry
https://www.readbyqxmd.com/read/27895113/age-dependent-absolute-abundance-of-hepatic-carboxylesterases-ces1-and-ces2-by-lc-ms-ms-proteomics-application-to-pbpk-modeling-of-oseltamivir-in-vivo-pharmacokinetics-in-infants
#11
Mikael Boberg, Marc Vrana, Aanchal Mehrotra, Robin E Pearce, Andrea Gaedigk, Deepak Kumar Bhatt, J Steven Leeder, Bhagwat Prasad
The age-dependent absolute protein abundance of carboxylesterase 1 and 2 (CES1 and CES2) in human liver was investigated and applied to predict infant pharmacokinetics (PK) of oseltamivir. The CES absolute protein abundance was determined by LC-MS/MS proteomics in human liver microsomal and cytosolic fractions prepared from tissue samples obtained from 136 pediatric and 35 adult donors. Two surrogate peptides per protein were selected for the quantification of CES1 and CES2 protein abundance. Purified CES1 and CES2 protein standards were used as calibrators, and the heavy labeled peptides were used as the internal standards...
November 28, 2016: Drug Metabolism and Disposition: the Biological Fate of Chemicals
https://www.readbyqxmd.com/read/27881673/sortilin-1-modulates-hepatic-cholesterol-lipotoxicity-in-mice-via-functional-interaction-with-liver-carboxylesterase-1
#12
Jibiao Li, Yifeng Wang, David J Matye, Hemantkumar Chavan, Partha Krishnamurthy, Feng Li, Tiangang Li
The liver plays a key role in cholesterol metabolism. Impaired hepatic cholesterol homeostasis causes intracellular free cholesterol accumulation and hepatocyte injury. Sortilin 1 (SORT1) is a lysosomal trafficking receptor that was identified by genome-wide association studies (GWAS) as a novel regulator of cholesterol metabolism in humans. Here we report that SORT1 deficiency protected against cholesterol accumulation-induced liver injury and inflammation in mice. Using an LC-MS/MS-based proteomics approach, we identified liver carboxylesterase 1 (CES1) as a novel SORT1-interacting protein...
January 6, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27831961/nomenclature-for-alleles-of-the-human-carboxylesterase-1-gene
#13
Henrik B Rasmussen, Majbritt B Madsen, Peter R Hansen
No abstract text is available yet for this article.
November 9, 2016: Pharmacogenetics and Genomics
https://www.readbyqxmd.com/read/27800573/pharmacokinetic-modeling-and-monte-carlo-simulation-to-predict-interindividual-variability-in-human-exposure-to-oseltamivir-and-its-active-metabolite-ro-64-0802
#14
Mototsugu Ito, Hiroyuki Kusuhara, Atsushi Ose, Tsunenori Kondo, Kazunari Tanabe, Hideki Nakayama, Shigeru Horita, Takuya Fujita, Yuichi Sugiyama
Oseltamivir (Tamiflu®) is a prodrug of Ro 64-0802, a selective inhibitor of influenza virus neuraminidase. There is a possible relationship between oseltamivir treatment and neuropsychiatric adverse events; although this has not been established, close monitoring is recommended on the prescription label. The objective of this study was to predict interindividual variability of human exposure to oseltamivir and its active metabolite Ro 64-0802. By leveraging mathematical models and computations, physiological parameters in virtual subjects were generated with population means and coefficient of variations collected from the literature or produced experimentally...
January 2017: AAPS Journal
https://www.readbyqxmd.com/read/27771568/effect-of-surfactant-in-mitigating-cadmium-oxide-nanoparticle-toxicity-implications-for-mitigating-cadmium-toxicity-in-environment
#15
Sricharani Rao Balmuri, Uthra Selvaraj, Vadivel Vinod Kumar, Savarimuthu Philip Anthony, Aristides Michael Tsatsakis, Kirill Sergeevich Golokhvast, Thiagarajan Raman
Cadmium (Cd), classified as human carcinogen, is an extremely toxic heavy metal pollutant, and there is an increasing environmental concern for cadmium exposure through anthropogenic sources including cigarette smoke. Though Cd based nanoparticles such as cadmium oxide (CdO) are being widely used in a variety of clinical and industrial applications, the toxicity of CdO nanoparticles has not been well characterized. Herein we report the toxicity of CdO nanoparticles employing zebrafish as a model. Two different CdO nanoparticles were prepared, calcination of Cd(OH)2 without any organic molecule (CdO-1) and calcination of Cd-citrate coordination polymer (CdO-2), to evaluate and compare the toxicity of these two different CdO nanoparticles...
January 2017: Environmental Research
https://www.readbyqxmd.com/read/27754602/population-pharmacokinetics-of-methylphenidate-in-healthy-adults-emphasizing-novel-and-known-effects-of-several-carboxylesterase-1-ces1-variants
#16
Y K Lyauk, C Stage, T K Bergmann, L Ferrero-Milliani, D Bjerre, R Thomsen, K P Dalhoff, H B Rasmussen, G Jürgens
The aim of this study was to identify demographic and genetic factors that significantly affect methylphenidate (MPH) pharmacokinetics (PK), and may help explain interindividual variability and further increase the safety of MPH. d-MPH plasma concentrations, demographic covariates, and carboxylesterase 1 (CES1) genotypes were gathered from 122 healthy adults and analyzed using nonlinear mixed effects modeling. The structural model that best described the data was a two-compartment disposition model with absorption transit compartments...
December 2016: Clinical and Translational Science
https://www.readbyqxmd.com/read/27733453/a-linkage-map-and-qtl-analysis-for-pyrethroid-resistance-in-the-bed-bug-cimex-lectularius
#17
Toby Fountain, Mark Ravinet, Richard Naylor, Klaus Reinhardt, Roger K Butlin
The rapid evolution of insecticide resistance remains one of the biggest challenges in the control of medically and economically important pests. Insects have evolved a diverse range of mechanisms to reduce the efficacy of the commonly used classes of insecticides, and finding the genetic basis of resistance is a major aid to management. In a previously unstudied population, we performed an F2 resistance mapping cross for the common bed bug, Cimex lectularius, for which insecticide resistance is increasingly widespread...
December 7, 2016: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/27728890/cyromazine-resistance-in-a-field-strain-of-house-flies-musca-domestica-l-resistance-risk-assessment-and-bio-chemical-mechanism
#18
Hafiz Azhar Ali Khan, Waseem Akram
Developing resistance management strategies for eco-friendly insecticides is essential for the management of insect pests without harming the environment. Cyromazine is a biorational insecticide with very low mammalian toxicity. Resistance to cyromazine has recently been reported in house flies from Punjab, Pakistan. In order to propose a resistance management strategy for cyromazine, experiments were planned to study risk for resistance development, possibility of cross-resistance and bio-chemical mechanisms...
January 2017: Chemosphere
https://www.readbyqxmd.com/read/27723570/in-vitro-and-in-vivo-studies-of-cholinesterases-and-carboxylesterases-in-planorbarius-corneus-exposed-to-a-phosphorodithioate-insecticide-finding-the-most-sensitive-combination-of-enzymes-substrates-tissues-and-recovery-capacity
#19
Sofía Otero, Gisela Kristoff
Organophosphate insecticides (OPs) continue to be an important class of agrochemicals used in modern agriculture worldwide. Even though these pesticides persist in the environment for a relatively short time, they show a high acute toxicity that may represent a serious hazard for wildlife. Sub-lethal effects on non-target species are a focus in pest management programs and should be used as biomarkers. Cholinesterases (ChEs) are the most used biomarker of OP exposure in vertebrate and invertebrate species. However, the combined monitoring of ChE and carboxylesterase (CE) activities may provide a more useful indication of exposure and effect of the organisms...
November 2016: Aquatic Toxicology
https://www.readbyqxmd.com/read/27720728/cloning-of-two-carboxylesterase-cdnas-from-the-swimming-crab-portunus-trituberculatus-molecular-evidences-for-their-putative-roles-in-methyl-farnesotae-degradation
#20
Tian Tao, Xi Xie, Mingxin Liu, Qinghua Jiang, Dongfa Zhu
The sesquiterpenoid methyl farnesoate (MF) is the unepoxidized form of insect juvenile hormone (JH) III, and is considered an equivalent of JH in crustaceans. Degradation of MF is similar to that of JH which occurs through ester hydrolysis by specific carboxylesterases (CXEs). In this study, the full-length cDNAs of two JH esterase-like CXEs were cloned from the swimming crab, Portunus trituberculatus. The predicted amino acid sequences of the two PtCXEs contain the conserved motifs including catalytic triad and oxyanion hole, which are the hallmark of the CXE family proteins...
January 2017: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
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