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https://www.readbyqxmd.com/read/28647508/fingerprint-efficacy-study-of-the-quaternary-alkaloids-in-corydalis-yanhusuo
#1
Qiuyue Li, Huanyu Guan, Xiaoming Wang, Yang He, Hong Sun, Wenting Tan, Xiaomei Luo, Meifeng Su, Yue Shi
ETHNOPHARMACOLOGICAL RELEVANCE: Corydalis yanhusuo is a well-known Chinese herbal medicine that is commonly applied as an analgesic agent in clinic using for thousands of years. Resent research showed that the quaternary ammonium alkaloids from Corydalis yanhusuo have a significant effect on myocardial ischemia. However, the corresponding anti-myocardial ischemia components that represent the efficacy of the quaternary ammonium alkaloids have not been elucidated. AIM OF THE STUDY: Explore the anti-myocardial ischemia components of Corydalis yanhusuo and develop a method of quality control for Corydalis yanhusuo...
June 21, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28647481/new-depsides-from-the-roots-of-salvia-miltiorrhiza-and-their-radical-scavenging-capacity-and-protective-effects-against-h2o2-induced-h9c2-cells
#2
Jianqing Zhang, Qinghao Jin, Yanping Deng, Jinjun Hou, Wanying Wu, Dean Guo
Phytochemical investigation of the roots of Salvia miltiorrhiza led to isolations of two new depsides (1-2), along with thirteen known compounds (3-15). Their structures and relative stereochemistry were elucidated by NMR spectral ((1)H and (13)C NMR, HSQC, HMBC), CD and HR-ESIMS data analyses. The absolute configuration of 1 was determined by comparison of the experimental and calculated ECD spectra. All the depsides (1-10) were found to exhibit stronger free radical scavenging capacity (ranging from 2.62 to 22...
June 21, 2017: Fitoterapia
https://www.readbyqxmd.com/read/28647341/suppression-of-autophagic-flux-contributes-to-cardiomyocyte-death-by-activation-of-necroptotic-pathways
#3
Makoto Ogasawara, Toshiyuki Yano, Masaya Tanno, Koki Abe, Satoko Ishikawa, Takayuki Miki, Atsushi Kuno, Toshiyuki Tobisawa, Shingo Muratsubaki, Kouhei Ohno, Yuki Tatekoshi, Kei Nakata, Wataru Ohwada, Tetsuji Miura
BACKGROUND: The role of necroptosis in myocardial injury has not been fully characterized. Here we examined roles of mitochondrial permeability transition pore (mPTP) and autophagy in necroptosis of cardiomyocytes. METHODS AND RESULTS: In H9c2 cells, necroptosis was induced by treatment with TNF-α (TNF) and z-VAD-fmk (zVAD) for 24h, and necroptotic death was determined by LDH release (as % of total). TNF/zVAD increased LDH release from 16.6±4.3% to 60.6±2.7%, and the LDH release was suppressed by necrostatin-1 (29...
June 21, 2017: Journal of Molecular and Cellular Cardiology
https://www.readbyqxmd.com/read/28643512/quantitatively-intrinsic-biomimetic-catalytic-activity-of-nanocerias-as-radical-scavengers-and-their-ability-against-h2o2-and-doxorubicin-induced-oxidative-stress
#4
Zhimin Tian, Xuhui Li, Yuanyuan Ma, Tao Chen, Dehui Xu, Bingchuan Wang, Yuan Gao, Yongquan Qu
Artificial enzymes as radical scavengers show great potentials in treatments on various diseases induced by oxidative stress. Herein, the quantitative analysis indicates the intrinsic activity of nanocerias for degradation of radicals is de-termined by the concentration of surface defects as well as their morphological features. The surface Ce3+ fraction of the CeO2 nanozymes with the similar morphology can be used as a descriptor to index their catalytic activity as radical scavengers. Defect-abundant porous nanorods of ceria (PN-CeO2) with a large surface area (141 m2/g) and high surface Ce3+ fraction (32...
June 23, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28643448/post-conditioning-with-metformin-attenuates-apoptotic-events-in-cardiomyoblasts-associated-with-ischemic-reperfusion-injury
#5
Rajesh Ramachandran, Mini S
Ischemic post-conditioning is considered as a potent method to combat ischemic reperfusion injury than pre-conditioning since the latter is non predictive and risky. Rapid initiation of reperfusion with potent anti oxidant compound can be one of the most effective treatments to reduce the infract size and behavioural deficits as a result of ischemic insult. Apoptosis/Necrotic events undisputedly hold significant role in determining the fate of cardiomyocytes post ischemic injury but current studies are speculative regrading the role of post-conditioning in maintaining cellular homeostasis...
June 23, 2017: Cardiovascular Therapeutics
https://www.readbyqxmd.com/read/28643395/catalase-ameliorates-diabetes-induced-cardiac-injury-through-reduced-p65-rela-mediated-transcription-of-becn1
#6
Xu Wang, Youli Tao, Yewei Huang, Kungao Zhan, Mei Xue, Ying Wang, Dandan Ruan, Yangzhi Liang, Xiaozhong Huang, Jianjun Lin, Zhiwei Chen, Lingchun Lv, Santie Li, Gen Chen, Yang Wang, Ruijie Chen, Weitao Cong, Litai Jin
Catalase is an antioxidative enzyme that converts hydrogen peroxide (H2 O2 ) produced by superoxide dismutase from highly reactive superoxide (O2(-) ) to water and oxygen molecules. Although recent findings demonstrate that catalase, autophagy and the nuclear factor κB (NF-κB) signalling pathway are centrally involved in diabetic cardiomyopathy (DCM), the interplay between the three has not been fully characterized. Thus, the mechanism responsible for catalase-mediated protection against heart injury in diabetic mice was investigated in this study, as well as the role of NF-κB-p65 in the regulation of autophagic flux was investigated in this study...
June 23, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28642708/myricitrin-protects-cardiomyocytes-from-hypoxia-reoxygenation-injury-involvement-of-heat-shock-protein-90
#7
Min Wang, Gui-Bo Sun, Yu-Yang Du, Yu Tian, Ping Liao, Xue-Song Liu, Jing-Xue Ye, Xiao-Bo Sun
Modulation of oxidative stress is therapeutically effective in ischemia/reperfusion (I/R) injury. Myricitrin, a naturally occurring phenolic compound, is a potent antioxidant. However, little is known about its effect on I/R injury to cardiac myocytes. The present study was performed to investigate the potential protective effect of myricitrin against hypoxia/reoxygenation (H/R)-induced H9c2 cardiomyocyte injury and its underlying mechanisms. Myricitrin pretreatment improved cardiomyocyte viability, inhibited ROS generation, maintained the mitochondrial membrane potential, reduced apoptotic cardiomyocytes, decreased the caspase-3 activity, upregulated antiapoptotic proteins and downregulated proapoptotic proteins during H/R injury...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28638481/neonatal-heart-enriched-mir-708-promotes-proliferation-and-stress-resistance-of-cardiomyocytes-in-rodents
#8
Shengqiong Deng, Qian Zhao, Lixiao Zhen, Chuyi Zhang, Cuicui Liu, Guangxue Wang, Lin Zhang, Luer Bao, Ying Lu, Lingyu Meng, Jinhui Lü, Ping Yu, Xin Lin, Yuzhen Zhang, Yi-Han Chen, Huimin Fan, William C Cho, Zhongmin Liu, Zuoren Yu
Adult heart has limited potential for regeneration after pathological injury due to the limited cell proliferation of cardiomyocytes and the quiescent status of progenitor cells. As such, induction of cell-cycle reentry of cardiomyocytes is one of the key strategies for regeneration of damaged heart. In this study, a subset of miRNAs including miR-708 were identified to be much more abundant in the embryonic and neonatal cardiomyocytes than that in adult rodents. Overexpression of miR-708 promoted cellular proliferation of H9C2 cells or primary cardiomyocytes from neonatal rats or mice in vitro...
2017: Theranostics
https://www.readbyqxmd.com/read/28637941/a-novel-danshensu-tetramethylpyrazine-conjugate-dt-010-provides-cardioprotection-through-the-pgc-1%C3%AE-nrf2-ho-1-pathway
#9
Xiaojing Zhang, Huihui Hu, Jingxiong Luo, Huixing Deng, Pei Yu, Zaijun Zhang, Gaoxiao Zhang, Luchen Shan, Yuqiang Wang
In this study, we investigated the cardioprotective mechanisms of action of DT-010, a novel danshensu-tetramethylpyrazine conjugate. DT-010 significantly preserved cell viability and suppressed cell apoptosis in H9c2 cells injured by tert-butylhydroperoxide (t-BHP), iodoacetic acid (IAA) and hypoxia-reoxygenation. In addition, DT-010 pre-treatment reduced the intracellular level of free radicals including superoxide anion (•O2(-)), hydroxyl radical (•OH) and peroxynitrite anion (ONOO(-)) after t-BHP exposure...
June 22, 2017: Biological & Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/28636290/sulforaphane-protection-against-the-development-of-doxorubicin-induced-chronic-heart-failure-is-associated-with-nrf2-upregulation
#10
Yang Bai, Qiang Chen, Yun-Peng Sun, Xuan Wang, Li Lv, Li-Ping Zhang, Jin-Sha Liu, Song Zhao, Xiao-Lu Wang
Doxorubicin (DOX) is an anthracycline anti-tumor drug. However, its clinical use is limited by dose-dependent cardiotoxicity, and even progresses to chronic heart failure (CHF). OBJECTIVE: This study aims to investigate whether the Nrf2 activator, sulforaphane (SFN), can prevent DOX-induced CHF. METHODS: Male Sprague Dawley rats who received treatment for six weeks were divided into four groups (n=30 per group): control, SFN, DOX, and DOX plus SFN group. RESULTS: Results revealed that DOX induced progressive cardiac damage as indicated by increased cardiac injury markers, cardiac inflammation, fibrosis and oxidative stress...
June 21, 2017: Cardiovascular Therapeutics
https://www.readbyqxmd.com/read/28632266/proteomic-analysis-reveals-xuesaitong-injection-attenuates-myocardial-ischemia-reperfusion-injury-by-elevating-pyruvate-dehydrogenase-mediated-aerobic-metabolism
#11
Xiaoping Zhao, Feng Zhang, Yi Wang
Xuesaitong injection (XST), which mainly consists of Panax notoginseng saponins, has been widely used for treating cardio-cerebral vascular diseases. However, the underlying mechanisms of XST associated with its cardioprotective effects are still unclear. To identify the potential target proteins of XST, two-dimensional gel electrophoresis (2-DE)-based proteomics was utilized to analyze the protein profile of myocardium in rats with myocardial ischemia/reperfusion (I/R) injury. The differentially expressed proteins were identified by matrix assisted laser desorption/ionization time-of-flight mass spectrometry...
June 20, 2017: Molecular BioSystems
https://www.readbyqxmd.com/read/28630041/dysregulated-zn-2-homeostasis-impairs-cardiac-type-2-ryanodine-receptor-and-mitsugumin-23-functions-leading-to-sarcoplasmic-reticulum-ca-2-leakage
#12
Benedict Reilly-O'Donnell, Gavin B Robertson, Angela Karumbi, Connor McIntyre, Wojciech Bal, Miyuki Nishi, Hiroshi Takeshima, Alan J Stewart, Samantha J Pitt
Aberrant Zn2+-homeostasis is associated with dysregulated intracellular Ca2+ release resulting in chronic heart failure. In the failing heart, a small population of cardiac ryanodine receptors (RyR2) display sub-conductance state gating leading to Ca2+ leakage from sarcoplasmic reticulum (SR) stores, which impairs cardiac contractility. Previous evidence suggests contribution of RyR2-independent Ca2+ leakage through an uncharacterized mechanism. We sought to examine the role of Zn2+ in shaping intracellular Ca2+ release in cardiac muscle...
June 19, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28629357/xuefuzhuyu-decoction-protected-cardiomyocytes-against-hypoxia-reoxygenation-injury-by-inhibiting-autophagy
#13
Xiaowen Shi, Haiyan Zhu, Yuanyuan Zhang, Mingmei Zhou, Danli Tang, Huamin Zhang
BACKGROUND: XuefuZhuyu decoction (XFZY) is a well-known traditional Chinese herbal medicine for the treatment of various cardiovascular diseases, such as unstable angina pectoris and myocardial ischemia-reperfusion injury. However, the mechanism by which XFZY contributes to the amelioration of cardiac injury remains unclear. METHODS: H9C2 cells were cultured under the hypoxic condition for 10 h and reoxygenated for 2 h. In the presence of various concentrations of XFZY for 12 h, the cell viability was measured by MTT assay...
June 19, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28629086/enhancing-internalization-of-silica-particles-in-myocardial-cells-through-surface-modification
#14
Nancy Ornelas-Soto, Rodrigo Rubio-Govea, Carlos E Guerrero-Beltrán, Eduardo Vázquez-Garza, Judith Bernal-Ramírez, Alejandra García-García, Yuriana Oropeza-Almazán, Gerardo García-Rivas, Flavio F Contreras-Torres
Surface modification in nanostructured mesoporous silica particles (MSNs) can significantly increase the uptake in myocardial cells. Herein, MSNs particles were synthesized and chemically functionalized to further assess their biocompatibility in rat myocardial cell line H9c2. The surface modification resulted in particles with an enhanced cellular internallization (3-fold increase) with respect to pristine particles. Apoptosis events were not evident at all, while necrosis incidence was significant only at a higher doses (>500μg/mL)...
October 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28627660/inhibition-of-autophagy-by-berberine-enhances-the-survival-of-h9c2-myocytes-following-hypoxia
#15
Zhuyin Jia, Lu Lin, Shanjun Huang, Zhouyang Zhu, Weijian Huang, Zhouqing Huang
Hypoxia may induce apoptosis and autophagy to promote cardiomyocyte injury. The present study investigated the effect of berberine, a natural extract of Rhizoma Coptidis, on hypoxia‑induced autophagy and apoptosis in the H9c2 rat myocardial cell line. Expression levels of apoptosis and autophagy markers were upregulated in H9c2 myocytes during hypoxia and cell viability was reduced. However, berberine significantly reduced hypoxia‑induced autophagy in H9c2 myocytes, as demonstrated by the ratio of microtubule‑associated proteins 1A/1B light chain 3 I/II and the expression levels of B‑cell lymphoma 2 (Bcl‑2)/adenovirus E1B 19 kDa protein‑interacting protein 3, and promoted cell viability...
June 14, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28627657/shrna%C3%A2-mediated-np-knockdown-inhibits-the-apoptosis-of-cardiomyocytes-induced-by-h1n1pdm2009-influenza-virus
#16
Kun Yu, Yi Ren, Xiwen Zhang, Taiping Qiao, Zhiguo Liu, Jianhua Shi, Yingnan Wang
Acute influenza-associated myocarditis varies in clinical severity ranging between asymptomatic and fulminant varieties. The most severe cases can result in impaired cardiac function‑associated mortality; however, the mechanism underlying the development of viral myocarditis has yet to be fully elucidated. The present study investigated the apoptosis induced in H9C2 cardiomyocytes by infection with the H1N1pdm2009 virus. The H9C2 cells were transfected with nucleoprotein (NP)‑specific short hairpin (sh) RNA, and viral replication was re‑evaluated in H9C2 cells infected with the H1N1pdm2009 virus, as was the apoptosis induced by the virus...
June 8, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28625734/synthesis-and-cardiomyocyte-protection-activity-of-crocetin-diamide-derivatives
#17
Jin Gao, Ming Chen, Xue-Cong Ren, Xiao-Bo Zhou, Qiang Shang, Wen-Qi Lu, Pei Luo, Zhi-Hong Jiang
A series of novel diamide derivatives (2-8) of crocetin (1) were synthesized and evaluated for their cardioprotective activity in vitro. Using well-established model of hypoxia-induced injury in H9c2 cells, we investigated the effects of 9 compounds and positive drug nicorandil on cellular cytotoxicity by MTT assay, mitochondrial viable staining, LDH activity and mitochondrial membrane potential (MMP). Among the new derivatives, compounds 3 and 4 with good liposolubility showed significantly potent activity than crocetin (1) against hypoxia-induced cytotoxicity...
June 15, 2017: Fitoterapia
https://www.readbyqxmd.com/read/28624452/fortunellin-protects-against-high-fructose-induced-diabetic-heart-injury-in-mice-by-suppressing-inflammation-and-oxidative-stress-via-ampk-nrf-2-pathway-regulation
#18
Cuihua Zhao, Yuan Zhang, Hongyang Liu, Peng Li, Han Zhang, Guanchang Cheng
Inflammation and oxidative stress contribute to the progression of diabetic cardiomyopathy (DCM). The study was first designed to calculate the role of an anti-inflammatory and anti-oxidant Fortunellin (For) in high fructose-induced cardiac injury in diabetic mice. Fortunellin was found to be none of toxicity to mice and cells using various assays. High fructose was used to induce mice with diabetes. The heart histopathological changes and cardiac function were measured. Fortunellin significantly attenuated the score of histopathological alterations and alleviated heart function, accompanied with reduced inflammation and oxidative stress...
June 15, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28624441/hsp90-inhibitor-geldanamycin-attenuates-the-cytotoxicity-of-sunitinib-in-cardiomyocytes-via-inhibition-of-the-autophagy-pathway
#19
Takayuki Kimura, Mai Uesugi, Kazuma Takase, Norimasa Miyamoto, Kohei Sawada
Sunitinib malate (sunitinib) is an orally available, multitargeted tyrosine kinase inhibitor with antitumor and antiangiogenic activities. Although sunitinib is effective for the treatment of patients with gastrointestinal stromal tumor, advanced renal cell carcinoma, or pancreatic neuroendocrine tumor, adverse cardiac events associated with sunitinib administration have been reported. Here, we examined the effect of geldanamycin, an inhibitor of heat shock protein (Hsp) 90, on sunitinib-induced cytotoxicity in cardiomyocytes...
June 14, 2017: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/28623750/lcz696-improves-cardiac-function-via-alleviating-drp1-mediated-mitochondrial-dysfunction-in-mice-with-doxorubicin-induced-dilated-cardiomyopathy
#20
Yan Xia, Zhangwei Chen, Ao Chen, Mingqiang Fu, Zhen Dong, Kai Hu, Xiangdong Yang, Yunzeng Zou, Aijun Sun, Juying Qian, Junbo Ge
AIMS: LCZ696, a novel angiotensin receptor neprilysin inhibitor, is effective in treating heart failure patients. Doxorubicin (DOX) is an effective antitumor medication but the cardiotoxicity limited its clinical use. In this study, we aimed to determine the effect of LCZ696 on DOX-induced cardiomyopathy in mice and in vitro and to explore related mechanisms focusing on fission protein dynamin-related protein 1 (Drp1). METHODS AND RESULTS: In human study, we found that myocardial fission protein Drp1 expression and its ser 616 phosphorylation were significantly increased in dilated cardiomyopathy (DCM) patients...
June 14, 2017: Journal of Molecular and Cellular Cardiology
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