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https://www.readbyqxmd.com/read/29786755/cardiac-progenitor-cell%C3%A2-derived-exosomes-promote-h9c2-cell-growth-via-akt-mtor-activation
#1
Shentang Li, Jie Jiang, Zuocheng Yang, Zhuoying Li, Xing Ma, Xin Li
Exosomes are cell‑derived vesicles released from a variety of mammalian cells that are involved in cell‑to‑cell signalling. It has been reported that cardiac progenitor cells (CPCs) derived from an adult heart are one of the most promising stem cell types for cardioprotection and repair. The mammalian target of rapamycin (mTOR) signalling pathway is a pivotal regulator in CPCs, therefore, CPC‑derived exosomes were used in the present study to investigate whether it can promote H9C2 cell growth through the protein kinase B (PKB, or Akt)/mTOR signalling pathway...
May 21, 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29782903/two-new-flavonoid-triterpene-saponin-meroterpenoids-from-clinopodium-chinense-and-their-protective-effects-against-anoxia-reoxygenation-induced-apoptosis-in-h9c2-cells
#2
Yin-Di Zhu, Rong-Chang Chen, Hong Wang, Hai Jiang, Xue-Li Huang, Mei-Ling Zhang, Ling-Yu Li, Zhen Hu, Xu-Dong Xu, Chao-Jie Wang, Xiao-Xia Ye, Jun-Shan Yang
Two new flavonoid-triterpene saponin meroterpenoids, clinoposides G (1) and H (2) were isolated from the aerial parts of Clinopodium chinense (Benth.) O. Kuntze. Their structures were elucidated through spectroscopic and electronic circular dichroism (ECD) analyses. Compounds 1 and 2 were evaluated for their protective effects against anoxia/reoxygenation(A/R)-induced injury in H9c2 cells. A/R treatment severely injured the H9c2 cells, which was accompanied by apoptosis. Both 1 and 2 pretreatment significantly inhibited cell injury and apoptosis, improved mitochondrial membrane potential, increased activities of antioxidant enzymes, and reduced the levels of the inflammatory cytokines...
May 18, 2018: Fitoterapia
https://www.readbyqxmd.com/read/29782637/luteolin-ameliorates-rat-myocardial-ischemia-reperfusion-injury-through-peroxiredoxin-ii-activation
#3
Bo Wei, Qiao Lin, Ya-Ge Ji, Yi-Can Zhao, Li-Na Ding, Wen-Juan Zhou, Li-Hua Zhang, Chuan-Yu Gao, Wen Zhao
BACKGROUND AND PURPOSE: Antioxidants provide a promising therapeutic effect for the cardiovascular disease. Luteolin (LUT), a polyphenolic bioflavonoid, is known to confer cardioprotection, although the underlying mechanism, especially the role of LUT on the antioxidant enzymes (e.g. peroxiredoxin family, PRXs), remains elusive. EXPERIMENTAL APPROACH: The current study was designed to evaluate the impact of LUT on MI/R injury in vivo and in vitro, as well as the underlying mechanism with a focus on PRXs signaling...
May 21, 2018: British Journal of Pharmacology
https://www.readbyqxmd.com/read/29774488/the-effect-of-oxygen-in-sirt3-mediated-myocardial-protection-a-proof-of-concept-study-in-cultured-cardiomyoblasts
#4
Philipp Diehl, Daniel S Gaul, Jonas Sogl, Ulrike Flierl, Darren Henstridge, Juergen Pahla, Heiko Bugger, Maximilian Y Emmert, Frank Ruschitzka, Christoph Bode, Thomas F Lüscher, Martin Moser, Christian M Matter, Karlheinz Peter, Stephan Winnik
Sirtuin 3 is a nicotinamide adenine dinucleotide dependent mitochondrial deacetylase that governs mitochondrial metabolism and oxidative defense. The demise in myocardial function following myocardial ischemia has been associated with mitochondrial dysfunction. Sirt3 maintains myocardial contractile function and protects from cardiac hypertrophy. The role of Sirt3 in ischemia is controversial. Our objective was to understand, under what circumstances Sirt3 is protective in different facets of ischemia, using an in vitro proof-of-concept approach based on simulated ischemia in cultured cardiomyoblasts...
May 17, 2018: Journal of Thrombosis and Thrombolysis
https://www.readbyqxmd.com/read/29771714/remifentanil-induces-cardio-protection-against-ischemia-reperfusion-injury-by-inhibiting-endoplasmic-reticulum-stress-through-the-maintenance-of-zinc-homeostasis
#5
Mingwei Sheng, Ge Zhang, Jiannan Wang, Qing Yang, Huanhuan Zhao, Xinxin Cheng, Zhelong Xu
BACKGROUND: Although it is well known that remifentanil (Rem) elicits cardiac protection against ischemia/reperfusion (I/R) injury, the underlying mechanism remains unclear. This study tested if Rem can protect the heart from I/R injury by inhibiting endoplasmic reticulum (ER) stress through the maintenance of zinc (Zn) homeostasis. METHODS: Isolated rat hearts were subjected to 30 minutes of regional ischemia followed by 2 hours of reperfusion. Rem was given by 3 consecutive 5-minute infusions, and each infusion was followed by a 5-minute drug-free perfusion before ischemia...
May 15, 2018: Anesthesia and Analgesia
https://www.readbyqxmd.com/read/29771430/senp3-protects-h9c2-cells-from-apoptosis-triggered-by-h-r-via-stat3-pathway
#6
Y Zhang, L-M Zheng, C-X Wang, J-M Gu, S Xue
OBJECTIVE: To investigate whether SENP3 protects H9C2 cells from apoptosis triggered by H/R through the signal transducer and activator of transcription 3 (STAT3) pathway. MATERIALS AND METHODS: Male C57BL mice were cultured and mouse models of myocardial I/RI were established. At the same time, cardiomyoblast H9C2 cell line of rat embryo was cultured. Reactive oxygen species (ROS) level was detected during H/R using 2',7'-dichlorofluorescein diacetate (DCFH) kit...
May 2018: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29770421/quantitative-proteomics-and-systems-analysis-of-cultured-h9c2-cardiomyoblasts-during-differentiation-over-time-supports-a-function-follows-form-model-of-differentiation
#7
Cynthia Kankeu, Kylie Clarke, Delphi Van Haver, Kris Gevaert, Francis Impens, Anna Dittrich, H Llewelyn Roderick, Egle Passante, Heinrich J Huber
The rat cardiomyoblast cell line H9C2 has emerged as a valuable tool for studying cardiac development, mechanisms of disease and toxicology. We present here a rigorous proteomic analysis that monitored the changes in protein expression during differentiation of H9C2 cells into cardiomyocyte-like cells over time. Quantitative mass spectrometry followed by gene ontology (GO) enrichment analysis revealed that early changes in H9C2 differentiation are related to protein pathways of cardiac muscle morphogenesis and sphingolipid synthesis...
May 17, 2018: Molecular omics
https://www.readbyqxmd.com/read/29766723/structure-activity-relationships-of-nitro-substituted-aroylhydrazone-iron-chelators-with-antioxidant-and-antiproliferative-activities
#8
Katerina Hruskova, Eliska Potuckova, Lukas Opalka, Tereza Hergeselova, Pavlina Haskova, Petra Kovarikova, Tomas Simůnek, Katerina Vavrova
Aroylhydrazone iron chelators such as salicylaldehyde isonicotinoyl hydrazone (SIH) protect various cells against oxidative injury and/or display antineoplastic activities. Previous studies have shown that a nitro-substituted hydrazone, namely, NHAPI, displayed markedly improved plasma stability, selective antitumor activity, and moderate antioxidant properties. In this study, we prepared four series of novel NHAPI derivatives and explored their iron chelation activities, anti- or pro-oxidant effects, protection against model oxidative injury in the H9c2 cell line derived from rat embryonic cardiac myoblasts, cytotoxicities to the corresponding noncancerous H9c2 cells, and antiproliferative activities against the MCF-7 human breast adenocarcinoma and HL-60 human promyelocytic leukemia cell lines...
May 16, 2018: Chemical Research in Toxicology
https://www.readbyqxmd.com/read/29763795/protective-effects-of-echinacoside-against-anoxia-reperfusion-injury-in-h9c2-cells-via-up-regulating-p-akt-and-slc8a3
#9
Min Chen, Xiaodong Wang, Bo Hu, Jian Zhou, Xin Wang, Wei Wei, Hua Zhou
Echinacoside is a natural ingredient with various pharmacological activities. In this study, we investigated the protective effects of echinacoside on cardiomyocytes (rat H9c2 cells) in an anoxia/reperfusion (A/R) model. Further, the regulatory function of sodium-calcium exchanger protein 3 (SLC8A3/NCX3) as well as the protein kinase B (AKT) signaling were studied. The present results indicated that echinacoside protected against A/R-induced apoptosis in a dose manner, which was characterized by a decrease in the apoptosis and caspase 3 protein levels in H9c2 cells...
May 12, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29763685/autophagy-plays-a-pro-survival-role-against-methamphetamine-induced-apoptosis-in-h9c2-cells
#10
Chao Zhao, Yong Mei, Xufeng Chen, Lei Jiang, Yunfei Jiang, Xu Song, Hang Xiao, Jun Wang, Jingsong Zhang
Methamphetamine (METH) is a commonly abused psychostimulant that can induce severe neurotoxicity. Cardiovascular injury caused by METH has recently gained increasing attention; however, the underlying mechanisms remain unclear. As autophagy has been shown to be associated with cell injury, the association between autophagy and METH-mediated cell apoptosis was investigated in the present study. METH treatment significantly increased the expression of two key autophagy proteins, i.e., Beclin-1 and LC3-II, in the cardiomyocyte cell line H9C2...
May 12, 2018: Toxicology Letters
https://www.readbyqxmd.com/read/29761913/functional-variants-in-the-lc3b-gene-promoter-in-acute-myocardial-infarction
#11
Feng Gao, Qiang Su, Wentao Yang, Shuchao Pang, Shuai Wang, Yinghua Cui, Jinguo Zhang, Bo Yan
Acute myocardial infarction (AMI) is a common disease mainly caused by atherosclerosis, for which genetic causes remain largely unknown. Recently, low frequency and rare genetic variants have been proposed as risk factors. Autophagy has been involved in many cellular processes, such as lipid metabolism and inflammation, and implicated in human diseases, including cardiovascular diseases. In previous studies, we have reported reduced levels of LC3B, a core protein and a marker for autophagy, in AMI patients...
May 15, 2018: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29761875/mir-130-aggravates-acute-myocardial-infarction-induced-myocardial-injury-by-targeting-ppar-%C3%AE
#12
Xianglin Chu, Yiqing Wang, Liewen Pang, Jiechun Huang, Xiaotian Sun, Xiaofeng Chen
Cardiac remodeling is a common pathophysiological change associated with acute myocardial infarction (AMI). Recent evidence indicates that microRNAs are strong posttranscriptional regulators which play an important role in regulating the microenvironment of myocardial tissue after AMI. In this study, we sought to explore the potential role and underlying mechanism of miR-130 in AMI. H9c2 cells were cultured under hypoxic conditions to simulate myocardial infarction. The influence of aberrantly expressed miR-130 on H9c2 cells under hypoxia was also estimated with RT-PCR, western blot and enzyme-linked immunosorbent assay...
May 15, 2018: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29761244/copper-tolfenamic-acid-evaluation-of-stability-and-anti-cancer-activity
#13
Myrna Hurtado, Umesh T Sankpal, Jaya Chhabra, Deondra T Brown, Rajasekhar Maram, Rafid Patel, Raj K Gurung, Jerry Simecka, Alvin A Holder, Riyaz Basha
The non-steroidal anti-inflammatory drug, Tolfenamic acid (TA) acts as an anti-cancer agent in several adult and pediatric cancer models. Copper (Cu) is an important element with multiple biological functions and has gained interest in medical applications. Recently, [Cu(TA)2 (bpy)] (Cu-TA) has been synthesized in order to enhance therapeutic activity. In this study, we synthesized Cu-TA using an established method, characterized it by UV visible spectroscopy and Fourier-transform infrared spectroscopy (FTIR), and tested its anti-cancer activity using twelve cell lines representing various cancers, such as Ewing sarcoma, glioblastoma, medulloblastoma, neuroblastoma, pancreatic and prostate...
May 15, 2018: Investigational New Drugs
https://www.readbyqxmd.com/read/29760296/hydroxytyrosol-and-olive-leaf-extract-exert-cardioprotective-effects-by-inhibiting-grp78-and-chop-expression
#14
Lixing Wu, Yuyu Xu, Zhijian Yang, Qing Feng
Myocardial infarction triggers massive biochemical changes, even cardiac cell death. Endoplasmic reticulum stress is involved in the pathology of myocardial infarction-mediated apoptosis. In the present study, myocardial cell line H9c2 cells were treated with cobalt chloride (CoCl2 ) to induce hypoxia. Isoproterenol was used for two successive days to induce myocardial infarction in SD rats. The cardioprotective effect of olive leaf extract (OLE) and its main constituent hydroxytyrosol and the underlying mechanisms were evaluated...
November 1, 2017: Journal of Biomedical Research
https://www.readbyqxmd.com/read/29758550/microrna-449a-inhibition-protects-h9c2-cells-against-hypoxia-reoxygenation-induced-injury-by-targeting-the-notch-1-signaling-pathway
#15
Jing Cheng, Qianfu Wu, Rong Lv, Li Huang, Banglong Xu, Xianbao Wang, Aihua Chen, Fei He
BACKGROUND/AIMS: The present study aimed to detect the expression of miR-449a and investigate the effect of miR-449a on cell injury in cardiomyocytes subjected to hypoxia/ reoxygenation (H/R) and its underlying mechanisms. METHODS: The expression of miR-449a was determined using reverse transcription-polymerase chain reaction in both neonatal rat ventricular myocytes and H9C2 cells. For gain-of-function and loss-of-function studies, H9C2 cells were transfected with either miR-449a mimics or miR-449a inhibitor...
May 7, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29758194/knockdown-of-trnau1ap-inhibits-the-proliferation-and-migration-of-nih3t3-jeg-3-and-bewo-cells-via-the-pi3k-akt-signaling-pathway
#16
Xiaoyan Hu, Jincheng Luo, Hehuan Lai, Mengdi Li, Xiaolin Zheng, Tingting Nie, Fenglan Li, Hui Li
The tRNA selenocysteine 1 associated protein 1 (Trnau1ap, initially named SECp43) is involved in Selenocysteine (Sec) biosynthesis and incorporation into selenoproteins, which play a key role in biological processes, such as embryonic development. We previously reported that downregulation of Trnau1ap inhibited proliferation of cardiomyocyte-like H9c2 cells. However, the effects of Trnau1ap on cell proliferation and migration of embryonic development are not known, and the mechanisms remain elusive. Herein, lentiviral shRNA vectors were transfected in NIH3T3, JEG-3 and Bewo cells (embryonic, trophoblast and placental cells)...
May 11, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29753072/ampk%C3%AE-2-deficiency-exacerbates-long-term-pm-2-5-exposure-induced-lung-injury-and-cardiac-dysfunction
#17
Hongyun Wang, Xiyue Shen, Guoxiong Tian, Xili Shi, Wei Huang, Yongguang Wu, Lei Sun, Can Peng, Shasha Liu, Ying Huang, Xiaoyu Chen, Fang Zhang, Yingjie Chen, Wenjun Ding, Zhongbing Lu
Previous studies have demonstrated that long-term exposure to fine particulate matter (PM2.5 ) increases the risk of respiratory and cardiovascular diseases. As a metabolic sensor, AMP-activated protein kinase (AMPK) is a promising target for cardiovascular disease. However, the impact of AMPK on the adverse health effects of PM2.5 has not been investigated. In this study, we exposed wild-type (WT) and AMPKα2-/- mice to either airborne PM2.5 (mean daily concentration ~64µg/m3 ) or filtered air for 6 months through a whole-body exposure system...
May 9, 2018: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/29752563/alamandine-attenuates-hypertension-and-cardiac-hypertrophy-in-hypertensive-rats
#18
Chi Liu, Chuan-Xi Yang, Xi-Ru Chen, Bo-Xun Liu, Yong Li, Xiao-Zhi Wang, Wei Sun, Peng Li, Xiang-Qing Kong
Oral administration of the peptide alamandine has antihypertensive and anti-fibrotic effects in rats. This work aimed to determine whether subcutaneous alamandine injection would attenuate hypertension and cardiac hypertrophy, and improve the function of a major target of hypertension-related damage, the left ventricle (LV), in spontaneously hypertensive rats (SHRs). This was examined in vivo in SHRs and normotensive rats subjected to 6-week subcutaneous infusion of alamandine or saline control, and in vitro in H9C2-derived and primary neonatal rat cardiomyocytes treated with angiotensin (Ang) II to model cardiac hypertrophy...
May 12, 2018: Amino Acids
https://www.readbyqxmd.com/read/29749426/-corrigendum-protective-effect-of-angiotensin%C3%A2-1%C3%A2-7-against-hyperglycaemia%C3%A2-induced-injury-in-h9c2-cardiomyoblast-cells-via-the-pi3k%C3%AC-akt-signaling-pathway
#19
Yi-Ying Yang, Xiu-Ting Sun, Zheng-Xun Li, Wei-Yan Chen, Xiang Wang, Mei-Ling Liang, Hui Shi, Zhi-Sheng Yang, Wu-Tao Zeng
Subsequently to the publication of this article, the authors have realized that an address affiliation associated with certain of the authors had been omitted. The authors' affiliation information should have appeared as follows (the omitted address affiliation is featured in bold): Yi‑Ying Yang1,2*, Xiu‑Ting Sun1,2*, Zheng‑Xun Li1,2, Wei‑Yan Chen3, Xiang Wang4, Mei‑Ling Liang5, Hui Shi1,2, Zhi‑Sheng Yang1,2 and Wu‑Tao Zeng1,2 1Department of Cardiology, The First Affiliated Hospital, Sun Yat‑Sen University; 2Key Laboratory on Assisted Circulation, Ministry of Health, Guangzhou, Guangdong 510080; 3Intensive Care Unit, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510260; 4Department of Cardiology, Laiwu City People's Hospital, Laiwu, Shandong 27110; 5Department of Cardiology, Sun Yat‑Sen Cardiovascular Hospital, Shenzhen, Guangdong 518020, P...
May 7, 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29745968/the-long-noncoding-rna-thril-knockdown-protects-hypoxia-induced-injuries-of-h9c2-cells-through-regulating-mir-99a
#20
Jingwen Xia, Nianxin Jiang, Yansong Li, Yong Wei, Xuan Zhang
BACKGROUND: Myocardial infarction (MI) is a leading cause of disease with high morbidity and mortality worldwide. Recent studies have revealed that long non-coding RNAs (lncRNAs) are involved in heart disease pathogenesis. This study aimed to investigate the effect and the molecular basis of THRIL on hypoxia-injured H9C2 cells. METHODS: THRIL, miR-99a and Brahma-related gene 1 (Brg1) expression in H9C2 cells were altered by transient transfections. The cells were subjected to hypoxia for 4 h, and then the levels of THRIL, miR-99a and Brg1 were investigated...
May 10, 2018: Cardiology Journal
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