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https://www.readbyqxmd.com/read/28092839/effect-and-mechanism-of-sorbus-pohuashanensis-hante-hedl-flavonoids-protect-against-arsenic-trioxide-induced-cardiotoxicity
#1
Xiaojin Yu, Zhenyu Wang, Zunpeng Shu, Zhengqing Li, Yuan Ning, Keli Yun, Haina Bai, Ruihai Liu, Wenli Liu
The cardiotoxicity of arsenic trioxide (ATO) limits its clinical application in cancer treatment. Evidences suggest that sorbus has antioxidant activity and its consumption has been linked with improved cardioprotection. In this study, we investigated the cardio-protective effect and mechanisms of Sorbus pohuashanensis (Hante) Hedl. flavonoids (SPF) against ATO in BALB/c mice and H9c2 cells. Eleven major flavonoids were confirmed by ultra-performance liquid chromatography electrospray ionization quadrupole time of flight mass spectrometry (UPLC-ESI-Q-TOF-MS)...
January 13, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28089921/penehyclidine-hydrochloride-prevents-anoxia-reoxygenation-injury-and-induces-h9c2-cardiomyocyte-apoptosis-via-a-mitochondrial-pathway
#2
Zhaoqi Wang, Duomao Lin, Liang Zhang, Wenjun Liu, Hongbao Tan, Jun Ma
Penehyclidine hydrochloride (PHC) is an anticholinergic drug that has been widely used in the clinic for years, evaluating for anesthetic premedication, anti-muscarinic, or improving microcirculation. However, very little is known about its protective effects against anoxia/reoxygenation (A/R) injury in myocardiocytes. The aim of our study was to investigate the protective effects of PHC pretreatment on A/R injury, as well as the underlying mechanisms involved. To investigate apoptosis, we used the cell viability assay, Annexin-V/PI assay, and lactate dehydrogenase (LDH) and creatine kinase (CK) activity measurements...
January 12, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/28088331/eicosapentaenoic-acid-ameliorates-palmitate-induced-lipotoxicity-via-the-amp-kinase-dynamin-related-protein-1-signaling-pathway-in-differentiated-h9c2-myocytes
#3
Atsushi Sakamoto, Masao Saotome, Prottoy Hasan, Terumori Satoh, Hayato Ohtani, Tsuyoshi Urushida, Hideki Katoh, Hiroshi Satoh, Hideharu Hayashi
BACKGROUND: Emerging evidence suggested the preferable effects of eicosapentaenoic acid (EPA; n-3 polyunsaturated fatty acid) against cardiac lipotoxicity, which worsens cardiac function by means of excessive serum free fatty acids due to chronic adrenergic stimulation under heart failure. Nonetheless, the precise molecular mechanisms remain elusive. In this study, we focused on dynamin-related protein-1 (Drp1) as a possible modulator of the EPA-mediated cardiac protection against cardiac lipotoxicity, and investigated the causal relation between AMP-activated protein kinase (AMPK) and Drp1...
January 11, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28071786/functional-and-cardioprotective-effects-of-simultaneous-and-individual-activation-of-protein-kinase-a-and-epac
#4
Igor Khaliulin, Mark Bond, Andrew F James, Zara Dyar, Raheleh Amini, Jason L Johnson, M-Saadeh Suleiman
BACKGROUND AND PURPOSE: Myocardial cAMP elevation confers cardioprotection against ischaemia/reperfusion (I/R) injury. cAMP activates two independent signalling pathways, PKA and Epac. This study investigated the cardiac effects of activating PKA and/or Epac and their involvement in cardioprotection against I/R. EXPERIMENTAL APPROACH: Hearts extracted from male rats were used either for determination of PKA and PKC activation or perfused in the Langendorff mode for either cardiomyocytes isolation or used to monitor functional activity at basal levels and after 30 min global ischaemia and 2 h reperfusion...
January 10, 2017: British Journal of Pharmacology
https://www.readbyqxmd.com/read/28068632/hydroxysafflor-yellow-a-cardioprotection-in-ischemia-reperfusion-i-r-injury-mainly-via-akt-hexokinase-ii-independent-of-erk-gsk-3%C3%AE-pathway
#5
Jia Min, Cui Wei
Hydroxysafflor yellow A (HSYA) is the main active component of Carthamus tinctorius L which has been used for hundreds of years in Chinese folk medicine in the treatment cardiovascular disease. This study was designed to investigate whether HSYA exerts cardioprotection in ischemia-reperfusion (I/R) injury heart and the mechanisms involved. The protective effect and mechanisms in myocardial ischemia reperfusion injury of HSYA was evaluated by hypoxia-recover (H/R) injury cell model which induced by hypoxia and recovered with oxygen in H9c2 cells...
January 6, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28051329/regulation-of-the-cardioprotective-adiponectin-and-its-receptor-adipor1-by-salt
#6
Nicholas Arnold, Abuzar Mahmood, Maya Ramdas, Paul P Ehlinger, Lakshmi Pulakat
Both circulating adiponectin (APN) and cardiac APN exert cardioprotective effects and improve insulin sensitivity and mitochondrial function. Low circulating APN serves as a biomarker for cardiovascular risk. Ablation of adiponectin receptor 1 (AdipoR1) causes myocardial mitochondrial dysfunction. Although high salt intake is a contributor to cardiovascular disease, how it modulates the expression of APN or AdipoR1 in cardiomyocytes is not known. We report that APN mRNA expression was attenuated in a dose-dependent manner in mouse cardiomyocyte cell line HL-1 exposed to salt concentrations ranging from 0...
November 30, 2016: Canadian Journal of Physiology and Pharmacology
https://www.readbyqxmd.com/read/28042316/localized-delivery-of-shrna-against-phd2-protects-the-heart-from-acute-myocardial-infarction-through-ultrasound-targeted-cationic-microbubble-destruction
#7
Li Zhang, Zhenxing Sun, Pingping Ren, Manjie You, Jing Zhang, Lingyun Fang, Jing Wang, Yihan Chen, Fei Yan, Hairong Zheng, Mingxing Xie
Hypoxia-inducible factor 1α (HIF-1α) plays a critical protective role in ischemic heart disease. Under normoxic conditions, HIF-1α was degraded by oxygen-dependent prolyl hydroxylase-2 (PHD2). Gene therapy has become a promising strategy to inhibit the degradation of HIF-1α and to improve cardiac function after ischemic injury. However, conventional gene delivery systems are difficult to achieve a targeted and localized gene delivery into the ischemic myocardia. Here, we report the localized myocardial delivery of shRNA against PHD2 through ultrasound-targeted microbubble destruction (UTMD) for protection the heart from acute myocardial infarction...
2017: Theranostics
https://www.readbyqxmd.com/read/28034286/altered-prolylcarboxypeptidase-expression-and-function-in-response-to-different-risk-factors-of-diabetes
#8
Tahmineh Tabrizian, Lillie Floyd, Zia Shariat-Madar
BACKGROUND: Prolylcarboxypeptidase (PRCP, EC:3.4.16.2) is a cardioprotective protease. Plasma PRCP levels are elevated in type 2 diabetes (T2D) mellitus and cardiovascular diseases. OBJECTIVE: Since diabetic cardiomyopathy is a late complication of uncontrolled diabetes, we tested the hypothesis that glucose and free fatty acid related risk factors for T2D mellitus and cardiovascular disease may reduce the cardioprotective property of PRCP. METHOD: 0 We examined the effects of glucose, saturated fatty acids, and unsaturated fatty acids on PRCP expression in cultured H9c2 cells as an in-vitro model for pharmacological studies...
December 29, 2016: Cardiovascular & Hematological Agents in Medicinal Chemistry
https://www.readbyqxmd.com/read/28033580/extracellular-cl-free-induced-cardioprotection-against-hypoxia-reoxygenation-is-associated-with-attenuation-of-mitochondrial-permeability-transition-pore
#9
Xian-Gui Zhang, Le Zhao, Yi Zhang, Yuan-Yuan Li, Huan Wang, Guang-Ling Duan, Lin Xiao, Xiao-Ran Li, He-Ping Chen
The isotonic substitution of extracellular chloride by gluconate (extracellular Cl(-)-free) has been demonstrated to elicit cardioprotection by attenuating ischaemia/reperfusion-induced elevation of intracellular chloride ion concentration ([Cl(-)]i). However, the downstream mechanism underlying the cardioprotective effect of extracellular Cl(-)-free is not fully established. Here, it was investigated whether extracellular Cl(-)-free attenuates mitochondrial dysfunction after hypoxia/reoxygenation (H/R) and whether mitochondrial permeability transition pore (mPTP) plays a key role in the extracellular Cl(-)-free cardioprotection...
December 26, 2016: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28017829/12-chloracetyl-ppd-a-novel-dammarane-derivative-shows-anti-cancer-activity-via-delay-the-progression-of-cell-cycle-g2-m-phase-and-reactive-oxygen-species-mediate-cell-apoptosis
#10
Xu De Wang, Yuan Yuan Sun, Chen Zhao, Fan Zhi Qu, Yu Qing Zhao
(20R)-Dammarane-3β, 12β, 20, 25-tetrol (25-OH-PPD) is a ginsenoside isolated from Panax ginseng (C. A. Meyer). This compound exhibits anti-cancer activities on many human cancer cell lines. In this study, we investigated anti-cancer mechanisms of 12β-O-(L-Chloracetyl)-dammar-20(22)-ene-3β,25-diol(12-Chloracetyl-PPD), a modified 25-OH-PPD. We found that compound 12-Chloracetyl-PPD resulted in a concentration-dependent inhibition of viability in prostate, breast, and gastric cancer cells, without affecting the viability of normal cell (human gastric epithelial cell line-GES-1, hair follicle dermal papilla cell line-HHDPC and rat myocardial cell line-H9C2)...
December 22, 2016: European Journal of Pharmacology
https://www.readbyqxmd.com/read/28013347/md2-mediates-angiotensin-ii-induced-cardiac-inflammation-and-remodeling-via-directly-binding-to-ang-ii-and-activating-tlr4-nf-%C3%AE%C2%BAb-signaling-pathway
#11
Jibo Han, Chunpeng Zou, Liqin Mei, Yali Zhang, Yuanyuan Qian, Shengban You, Yong Pan, Zheng Xu, Bin Bai, Weijian Huang, Guang Liang
Angiotensin II (Ang II) induces cardiac inflammation and remodeling. Emerging evidence indicates that Ang II may utilize the Toll-like receptor 4 (TLR4) signaling pathway in mediating pro-inflammatory and pro-fibrotic activities. However, the precise mechanism is poorly understood. Myeloid differentiation 2 (MD2), a molecule that physically binds to TLR4, confers lipopolysaccharide responsiveness and may also be involved in mediating the actions of Ang II. We hypothesize that MD2 plays an essential role in cardiac inflammation and remodeling induced by local Ang II, and inhibition of MD2 can attenuate Ang II-induced cardiac dysfunction...
January 2017: Basic Research in Cardiology
https://www.readbyqxmd.com/read/28011583/association-of-serum-hmgb2-level-with-mace-at-1-month-of-myocardial-infarction-aggravation-of-myocardial-ischemic-injury-in-rat-by-hmgb2-via-ros
#12
Zhu Hui Liu, Dao Peng Dai, Feng Hua Ding, Wen Qi Pan, Yue Hua Fang, Qi Zhang, Man Li, Ping Yang, Xiao Qun Wang, Ying Shen, Ling Jie Wang, Xiao Xiang Yan, Yu Hu He, Ke Yang, Rui Yan Zhang, Wei Feng Shen, Ying Chen, Lin Lu
BACKGROUND: High-mobility group box (HMGB) family is related to inflammatory diseases. We investigated whether serum HMGB2 levels are related to myocardial infarction (MI) severity and major adverse cardiac events (MACE) during MI. METHODS AND RESULTS: We included 432 consecutive patients with ST-segment elevation myocardial infarction and 312 controls. Serum HMGB2 levels were significantly higher in MI patients than in controls. Increased HMGB2 levels were associated with MACE and negatively with ejection fraction in MI patients...
December 23, 2016: American Journal of Physiology. Heart and Circulatory Physiology
https://www.readbyqxmd.com/read/28009721/propofol-provides-cardiac-protection-by-suppressing-the-proteasome-degradation-of-caveolin-3-in-ischemic-reperfused-rat-hearts
#13
Afang Zhu, Xin Wei, Yali Zhang, Tao You, Shanglong Yao, Shiying Yuan, Haodong Xu, Faqian Li, Weike Mao
The mechanisms underlying propofol's cardioprotective role remain elusive. Caveolin-3 (Cav-3) has been shown to mediate both opioids- and volatile anesthetics-induced cardioprotection against ischemia/reperfusion (I/R) injury. We hypothesize that the cardioprotective role of propofol is mediated through Cav-3 and its regulation of PI3K/Akt/GSK3β signal pathway. Rats or H9c2 cardiomyocytes were exposed to propofol prior to I/R or simulated ischemia/reperfusion (SI/R). Propofol pretreatment significantly decreased left ventricle (LV) infarct size in vivo (P < 0...
December 17, 2016: Journal of Cardiovascular Pharmacology
https://www.readbyqxmd.com/read/28006933/nilotinib-induces-er-stress-and-cell-death-in-h9c2-cells
#14
D Lekes, I Szadvari, O Krizanova, K Lopusna, I Rezuchova, M Novakova, Z Novakova, T Parak, P Babula
Tyrosine kinases inhibitors (TKi) represent a relatively novel class of anticancer drugs that target cellular pathways overexpressed in certain types of malignancies, such as chronic myeloid leukaemia (CML). Nilotinib, ponatinib and imatinib exhibit cardiotoxic and vascular effects. In this study, we focused on possible cardiotoxicity of nilotinib using H9c2 cells as a suitable cell model. We studied role of endoplasmic reticulum (ER) stress and apoptosis in nilotinib toxicity using a complex approach. Nilotinib impaired mitochondrial function and induced formation of ROS under clinically relevant concentrations...
December 21, 2016: Physiological Research
https://www.readbyqxmd.com/read/28006775/fgf-2-transcriptionally-down-regulates-the-expression-of-bnip3l-via-pi3k-akt-foxo3a-signaling-and-inhibits-necrosis-and-mitochondrial-dysfunction-induced-by-high-concentrations-of-hydrogen-peroxide-in-h9c2-cells
#15
Qian Chen, Xiaosong Chen, Conghui Han, Ying Wang, Tao Huang, Yingdong Du, Zhen Dong
BACKGROUND/AIMS: Cardiovascular disease is a growing major global public health problem. Necrosis is one of the main forms of cardiomyocyte death in heart disease. Oxidative stress is regarded as one of the key regulators of cardiac necrosis, which eventually leads to cardiovascular disease. Many pharmacological and in vitro studies have suggested that FGF-2 can act directly on cardiomyocytes to maintain the integrity and function of the myocardium and prevent damage during oxidative stress...
2016: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28000860/downregulation-of-the-%C3%AE-1-adrenergic-receptor-in-the-myocardium-results-in-insensitivity-to-metoprolol-and-reduces-blood-pressure-in-spontaneously-hypertensive-rats
#16
Yun Huang, Xiao-Li Liu, Jia Wen, Li-Hua Huang, Yao Lu, Ru-Jia Miao, Xing Liu, Ying Li, Xiao-Wei Xing, Hong Yuan
The β1‑adrenergic receptor (AR) is the primary β‑AR subtype in the heart and is the target of metoprolol (Met), which is commonly used to treat angina and hypertension. Previous studies have revealed a positive correlation between the methylation levels of the adrenoreceptor β1 gene (Adrb1) promoter in the myocardium with the antihypertensive activity of Met in spontaneously hypertensive rats (SHR), which affects β1‑AR expression in H9C2 cells. The aim of the present study was to investigate the effects of myocardial β1‑AR downregulation using short‑hairpin RNA (shRNA) against Adrb1 on the antihypertensive activity of Met in SHR...
February 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28000081/rotavirus-nsp486-175-interacts-with-h9c2-2-1-cells-in-vitro-elevates-intracellular-ca-2-levels-and-can-become-cytotoxic-a-possible-mechanism-for-extra-intestinal-pathogenesis
#17
Xiaoshun Xiong, Yinyin Hu, Caixia Liu, Xiangyang Li
Rotavirus (RV) is the predominant cause of infantile gastroenteritis with multiple pathogenic factors, among which enterotoxin NSP4 is the most significant factor. NSP4 has been shown to induce elevation of the intracellular calcium concentration, alteration of the cytoskeleton organization, and cytopathic effect among other processes. However, increasing evidence suggests that RVs can escape from the gastrointestinal tract and invade other organs and tissues to cause extra-intestinal diseases. In this study, we investigated whether NSP4 has a pathogenic effect on extra-intestinal cells and examined possible molecular mechanisms in vitro...
December 20, 2016: Virus Genes
https://www.readbyqxmd.com/read/27998959/exogenous-h2s-regulates-endoplasmic-reticulum-mitochondria-cross-talk-to-inhibit-apoptotic-pathways-in-stz-induced-type-i-diabetes
#18
Fan Yang, Xiangjing Yu, Ting Li, Jianjun Wu, YaJun Zhao, Aili Sun, Shiyun Dong, Jichao Wu, Xin Zhong, Changqing Xu, Fanghao Lu, Weihua Zhang
BACKGROUND: The upregulation of reactive oxygen species (ROS) is a primary cause of cardiomyocyte apoptosis in Diabetes cardiomyopathy (DCM). Mitofusin-2 (Mfn-2) is a key protein that bridges the mitochondria and endoplasmic reticulum(ER). Hydrogen sulfide (H2S)-mediated cardioprotection is related to antioxidant effects. The present study demonstrated that H2S inhibited the interaction between the ER and mitochondrial apoptotic pathway. METHODS: This study investigated cardiac function, ultrastructural changes in the ER and mitochondria, apoptotic rate using TUNEL and the expression of ER stress-associated proteins and mitochondrial apoptotic proteins in cardiac tissues in STZ-induced type I diabetic rats treated with or without NaHS (donor of H2S)...
December 20, 2016: American Journal of Physiology. Endocrinology and Metabolism
https://www.readbyqxmd.com/read/27997926/exogenous-hydrogen-sulfide-attenuates-high-glucose-induced-cardiotoxicity-by-inhibiting-nlrp3-inflammasome-activation-by-suppressing-tlr4-nf-%C3%AE%C2%BAb-pathway-in-h9c2-cells
#19
Zena Huang, Xiaodong Zhuang, Chuli Xie, Xun Hu, Xiaobian Dong, Yue Guo, Shaofen Li, Xinxue Liao
BACKGROUND/AIMS: This study aimed to investigate whether exogenous hydrogen sulfide (H2S) confered cardiac protection against high glucose (HG)-induced injury by inhibiting NLRP3 inflammasome activation via a specific TLR4/NF-κB pathway. METHODS: H9c2 cardiac cells were exposed to 33 mM glucose for 24 h to induce HG-induced cytotoxicity. The cells were pretreated with NaHS (a donor of H2S) before exposure to HG. Cell viability, cell apoptosis, intracellular reactive oxygen species (ROS), mitochondrial membrane potential (MMP), and TLR4, NF-κB, NLRP3 inflammasome, IL-1β, IL-18 and caspase-3 expression were measured by standard methods...
2016: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/27990618/lipid-emulsion-inhibits-apoptosis-induced-by-a-toxic-dose-of-verapamil-via-the-delta-opioid-receptor-in-h9c2-rat-cardiomyoblasts
#20
Seong-Ho Ok, Mun Hwan Choi, Il-Woo Shin, Soo Hee Lee, Sebin Kang, Jiah Oh, Jeong Yeol Han, Ju-Tae Sohn
The goals of this study were to investigate the effects of lipid emulsion (LE) on apoptosis induced by a toxic dose of verapamil in H9c2 cells and to elucidate the associated cellular mechanism. The effects of LE alone and combined with an inhibitor on the decreases in cell counts and viability induced by verapamil and diltiazem were examined using the MTT assay. The effects of verapamil alone, combined LE and verapamil treatment, and combined inhibitor, LE and verapamil treatment on cleaved caspase-3, caspase-8 and Bax expression, were examined using Western blotting...
December 19, 2016: Cardiovascular Toxicology
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