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Microparticle

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https://www.readbyqxmd.com/read/28214330/automated-microfluidic-sorting-of-mammalian-cells-labeled-with-magnetic-microparticles-for-those-that-efficiently-express-and-secrete-a-protein-of-interest
#1
Xuan Droz, Niamh Harraghy, Etienne Lançon, Valérie Le Fourn, David Calabrese, Thierry Colombet, Pascal Liechti, Amar Rida, Pierre-Alain Girod, Nicolas Mermod
We developed a method for the fast sorting and selection of mammalian cells expressing and secreting a protein at high levels. This procedure relies on cell capture using an automated microfluidic device handling antibody-coupled magnetic microparticles and on a timed release of the cells from the microparticles after capture. Using clinically compatible materials and procedures, we show that this approach is able to discriminate between cells that truly secrete high amounts of a protein from those that just display it at high levels on their surface without properly releasing it...
February 18, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28213977/mitochondria-targeted-esculetin-inhibits-pai-1-levels-by-modulating-stat3-activation-and-mir-19b-via-sirt3-role-in-acute-coronary-artery-syndrome
#2
Sujana Katta, Santosh Karnewar, Devayani Panuganti, Mahesh Kumar Jerald, Sastry B K, Srigiridhar Kotamraju
In this study, we explored the microRNAs responsible for the regulation of PAI-1 during LPS-stimulated inflammation in human aortic endothelial cells, subsequently studied the effect of a newly synthesized mitochondria-targeted esculetin (Mito-Esc) that was shown for its anti-atherosclerotic potential in modulating PAI-1 levels and its targeted miRs during angiotensin-II-induced atherosclerosis in ApoE(-/-) mice. LPS-stimulated PAI-1 was accompanied with an upregulation of miR-19b and down-regulation of miR-30c...
February 18, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28213842/determination-of-the-porosity-of-plga-microparticles-by-tracking-their-sedimentation-velocity-using-a-flow-imaging-microscope-flowcam
#3
A S Sediq, S K D Waasdorp, M R Nejadnik, M M C van Beers, J Meulenaar, R Verrijk, W Jiskoot
PURPOSE: To investigate whether particle sedimentation velocity tracking using a flow imaging microscope (FlowCAM) can be used to determine microparticle porosity. METHODS: Two different methods were explored. In the first method the sedimentation rate of microparticles was tracked in suspending media with different densities. The porosity was calculated from the average apparent density of the particles derived by inter- or extrapolation to the density of a suspending medium in which the sedimentation velocity was zero...
February 17, 2017: Pharmaceutical Research
https://www.readbyqxmd.com/read/28213360/circulating-endothelial-microparticles-and-mir-92a-in-acute-myocardial-infarction
#4
Yuchen Zhang, Junjun Cheng, Fang Chen, Changyan Wu, Junmeng Zhang, Xuejun Ren, Yu Pan, Bin Nie, Quan Li, Yu Li
Background : Microparticles (MPs) and microRNAs (miRNAs) have been shown to play important roles in coronary artery disease (CAD) by monitoring endothelial dysfunction. This study aims to investigate the diagnostic value of endothelial microparticles (EMPs) and miRNAs (miR-92a or miR-23a) as biomarkers in distinguishing patients with acute myocardial infarction (AMI) from those with CAD. Methods : Plasma samples from 37 patients with AMI, 42 patients with stable coronary artery disease (SCAD) and 35 healthy adults were collected for investigation in this study...
February 17, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28211454/harnessing-catalytic-pumps-for-directional-delivery-of-microparticles-in-microchambers
#5
Sambeeta Das, Oleg E Shklyaev, Alicia Altemose, Henry Shum, Isamar Ortiz-Rivera, Lyanne Valdez, Thomas E Mallouk, Anna C Balazs, Ayusman Sen
The directed transport of microparticles in microfluidic devices is vital for efficient bioassays and fabrication of complex microstructures. There remains, however, a need for methods to propel and steer microscopic cargo that do not require modifying these particles. Using theory and experiments, we show that catalytic surface reactions can be used to deliver microparticle cargo to specified regions in microchambers. Here reagents diffuse from a gel reservoir and react with the catalyst-coated surface. Fluid density gradients due to the spatially varying reagent concentration induce a convective flow, which carries the suspended particles until the reagents are consumed...
February 17, 2017: Nature Communications
https://www.readbyqxmd.com/read/28211364/antibacterial-effect-of-novel-biodegradable-and-bioresorbable-plda-mg-composites
#6
M C Fernández-Calderón, S C Cifuentes, M A Pacha-Olivenza, A M Gallardo-Moreno, L Saldaña, J L González-Carrasco, M T Blanco, N Vilaboa, M L González-Martín, C Pérez-Giraldo
Polylactic acid/Mg composites have been recently proposed for biodegradable osteosynthesis devices because, with regards to the neat polymer, they combine an enhanced biocompatibility and bioactivity with better mechanical properties, particularly creep strength. A question still arises about their bacterial behavior. For this purpose, composites of poly-L-D-lactic acid (PLDA) loaded with 1 and 10 wt.% of Mg microparticles were evaluated using Staphylococcus epidermidis, with special emphasis on the study of bacterial adhesion and biofilm formation...
February 17, 2017: Biomedical Materials
https://www.readbyqxmd.com/read/28210898/flow-cytometric-analysis-reveals-the-high-levels-of-platelet-activation-parameters-in-circulation-of-multiple-sclerosis-patients
#7
Agnieszka Morel, Joanna Rywaniak, Michał Bijak, Elżbieta Miller, Marta Niwald, Joanna Saluk
The epidemiological studies confirm an increased risk of cardiovascular disease in multiple sclerosis, especially prothrombotic events directly associated with abnormal platelet activity. The aim of our study was to investigate the level of blood platelet activation in the circulation of patients with chronic phase of multiple sclerosis (SP MS) and their reactivity in response to typical platelets' physiological agonists. We examined 85 SP MS patients diagnosed according to the revised McDonald's criteria and 50 healthy volunteers as a control group...
February 16, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28209527/exploring-deformable-particles-in-vascular-targeted-drug-delivery-softer-is-only-sometimes-better
#8
Margaret B Fish, Catherine A Fromen, Genesis Lopez-Cazares, Alexander W Golinski, Timothy F Scott, Reheman Adili, Michael Holinstat, Omolola Eniola-Adefeso
The ability of vascular-targeted drug carriers (VTCs) to localize and bind to a targeted, diseased endothelium determines their overall clinical utility. Here, we investigate how particle modulus and size determine adhesion of VTCs to the vascular wall under physiological blood flow conditions. In general, deformable microparticles (MPs) outperformed nanoparticles (NPs) in all experimental conditions tested. Our results indicate that MP modulus enhances particle adhesion in a shear-dependent manner. In low shear human blood flow profiles in vitro, low modulus particles showed favorable adhesion, while at high shear, rigid particles showed superior adhesion...
February 4, 2017: Biomaterials
https://www.readbyqxmd.com/read/28208348/propelling-and-spinning-of-microsheets-in-nematic-liquid-crystals-driven-by-ac-electric-field
#9
M V Rasna, U V Ramudu, R Chandrasekar, Surajit Dhara
Dynamics of microparticles in isotropic liquids by transducing the energy of an applied electric field have been studied for decades. Recently, such studies in anisotropic media like liquid crystals have opened up new perspectives in colloid science. Here, we report studies on ac-electric-field-driven dynamics of microsheets in nematic liquid crystals. In planar aligned liquid crystals, with negative dielectric anisotropy, the microsheets are propelled parallel to the director. A steady spinning of the microsheets is observed in homeotropic cells with positive dielectric anisotropy liquid crystals...
January 2017: Physical Review. E
https://www.readbyqxmd.com/read/28207887/platelet-monocyte-derived-and-tissue-factor-carrying-circulating-microparticles-are-related-to-acute-myocardial-infarction-severity
#10
Gemma Chiva-Blanch, Kristian Laake, Peder Myhre, Vibeke Bratseth, Harald Arnesen, Svein Solheim, Lina Badimon, Ingebjørg Seljeflot
OBJECTIVE: Circulating microparticles (cMPs) are phospholipid-rich vesicles released from cells when activated or injured, and contribute to the formation of intracoronary thrombi. Tissue factor (TF, CD142) is the main trigger of fibrin formation and TF-carrying cMPs are considered one of the most procoagulant cMPs. Similar types of atherosclerotic lesions may lead to different types of AMI, although the mechanisms behind are unresolved. Therefore, we aimed to investigate the phenotype of cMPs found in plasma of ACS patients and its relation to AMI severity and thrombotic burden...
2017: PloS One
https://www.readbyqxmd.com/read/28205314/comparison-of-lc-ms-ms-vs-chemiluminescent-microparticle-immunoassay-in-measuring-the-valproic-acid-concentration-in-plasma-of-epilepsy-patients-in-a-new-perspective
#11
Zhipeng Wang, Yunlei Yun, Xinfang Xie, Chunhua You, Haijun Miao, Feng Zhang, Shouhong Gao, Wansheng Chen
OBJECTIVES: This study was designed to compare the performance of LC-MS/MS with chemiluminescent microparticle immunoassay (CMIA) for determination of VPA in epilepsy patients in the perspective of metabolites' hepatotoxicity. METHOD: Samples were collected and then analyzed using both LC-MS/MS and CMIA. A LO2 cells (normal human hepatic cells) experiment was carried out to confirm VPA metabolites' hepatotoxicity using AST(Aspertate Aminotransferase, AST), ALT(Alanine aminotransferase, ALT) and LDH(lactate dehydrogenase, LDH) in supernate as index...
February 15, 2017: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/28205143/development-of-a-gastric-absorptive-immediate-responsive-oral-protein-loaded-versatile-polymeric-delivery-system
#12
Pierre P D Kondiah, Yahya E Choonara, Lomas K Tomar, Charu Tyagi, Pradeep Kumar, Lisa C du Toit, Thashree Marimuthu, Girish Modi, Viness Pillay
A multifunctional platform to deliver three diverse proteins of insulin, interferon beta (INF-β) and erythropoietin (EPO), using a novel copolymeric microparticulate system of TMC-PEGDMA-MAA, was synthesised as an intelligent pH-responsive 2-fold gastric and intestinal absorptive system. Physiochemical and physicomechanical studies proved the degree of crystallinity that supported the controlled protein delivery of the microparticulate system. The copolymer was tableted before undertaking in vitro and in vivo analysis...
February 15, 2017: AAPS PharmSciTech
https://www.readbyqxmd.com/read/28202792/towards-a-new-strategy-for-the-diagnosis-of-congenital-trypanosoma-cruzi-infection
#13
Alba Abras, Carmen Muñoz, Cristina Ballart, Pere Berenguer, Teresa Llovet, Mercedes Herrero, Silvia Tebar, María-Jesús Pinazo, Elizabeth Posada, Carmen Martí, Victoria Fumadó, Jordi Bosch, Oriol Coll, Teresa Juncosa, Gemma Ginovart, Josep Armengol, Joaquim Gascón, Montserrat Portús, Montserrat Gállego
The immigration of Latin American women of childbearing age has spread the congenital transmission of Chagas disease to non-endemic areas, and the disease is now a worldwide problem. Some European health authorities have implemented screening programs to prevent vertical transmission, but the lack of a uniform protocol calls for the urgent establishment of a new strategy, common for all laboratories. Our aims were (i) to analyze the trend of passive IgG antibodies in the newborn by means of five serological tests for the diagnosis and follow-up of congenital Trypanosoma cruzi infection, (ii) to assess the utility of these techniques for diagnosing a congenital transmission, and (iii) to propose a strategy for a prompt, efficient and cost-effective diagnosis of T...
February 15, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28199885/evaluation-of-polyesteramide-pea-and-polyester-plga-microspheres-as-intravitreal-drug-delivery-systems-in-albino-rats
#14
Tobias Peters, Seong-Woo Kim, Vinicius Castro, Krunoslav Stingl, Torsten Strasser, Sylvia Bolz, Ulrich Schraermeyer, George Mihov, MengMeng Zong, Vanessa Andres-Guerrero, Rocio Herrero Vanrell, Aylvin A Dias, Neil R Cameron, Eberhart Zrenner
PURPOSE: To study the suitability of injectable microspheres based on poly(ester amide) (PEA) or poly lactic-co-glycolic acid (PLGA) as potential vehicles for intravitreal drug delivery in rat eyes. Dexamethasone-loaded PEA microspheres (PEA + DEX) were also evaluated. METHODS: Forty male Sprague Dawley rats were divided into four groups that received different intravitreally injected microspheres: PEA group (n = 12); PLGA group (n = 12); PEA + DEX group (n = 8); and control group (no injection, n = 8)...
February 7, 2017: Biomaterials
https://www.readbyqxmd.com/read/28198852/influence-of-the-background-medium-on-imaging-performance-of-microsphere-assisted-super-resolution-microscopy
#15
Arash Darafsheh
We demonstrated the importance of the refractive index of the background medium containing the microsphere in microsphere-assisted super-resolution microscopy. We performed numerical simulation and showed that the photonic nanojet properties of a microlens can be optimized by adjusting the index of the background medium surrounding it. Our simulation results, in qualitative agreement with experimental observations, demonstrated that for the same refractive index contrast between the microsphere and its surrounding medium, a high-index microsphere embedded in a medium provides sharper focusing and better imaging quality compared with its low-index counterpart working in air...
February 15, 2017: Optics Letters
https://www.readbyqxmd.com/read/28198362/covalent-assembly-of-nanoparticles-as-a-peptidase-degradable-platform-for-molecular-mri
#16
Francisco Perez-Balderas, Sander I van Kasteren, Alaa A A Aljabali, Kim Wals, Sébastien Serres, Andrew Jefferson, Manuel Sarmiento Soto, Alexandre A Khrapitchev, James R Larkin, Claire Bristow, Seung Seo Lee, Guillaume Bort, Filippo De Simone, Sandra J Campbell, Robin P Choudhury, Daniel C Anthony, Nicola R Sibson, Benjamin G Davis
Ligand-conjugated microparticles of iron oxide (MPIO) have the potential to provide high sensitivity contrast for molecular magnetic resonance imaging (MRI). However, the accumulation and persistence of non-biodegradable micron-sized particles in liver and spleen precludes their clinical use and limits the translational potential of MPIO-based contrast agents. Here we show that ligand-targeted MPIO derived from multiple iron oxide nanoparticles may be coupled covalently through peptide linkers that are designed to be cleaved by intracellular macrophage proteases...
February 15, 2017: Nature Communications
https://www.readbyqxmd.com/read/28195290/microbubbles-trigger-oscillation-of-crystal-size-in-solids
#17
Anna Kollath, Nadzeya Brezhneva, Ekaterina V Skorb, Daria V Andreeva
An understanding of the nature and conditions of nonlinear processes in open systems is important for modulation of the microstructure of solids at a new level of complexity. We demonstrate that cavitation generated by high intensity ultrasound (HIUS) triggers nonlinear processes in microparticles and layers of titanium. We reveal a non-monotonic dependence of the size of grains in the treated solids on sonication time, and oscillation of titanium grain sizes vs. time of ultrasonic treatment, indicating the influence of two opposing forces: cavitation driven impact of shock heating and shear stress on surfaces...
February 14, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28194850/cannabidiol-reduces-ethanol-consumption-motivation-and-relapse-in-mice
#18
Adrián Viudez-Martínez, María S García-Gutiérrez, Carmen María Navarrón, María Isabel Morales-Calero, Francisco Navarrete, Ana Isabel Torres-Suárez, Jorge Manzanares
This study evaluated the effects of cannabidiol (CBD) on ethanol reinforcement, motivation and relapse in C57BL/6 J mice. The effects of CBD (60 mg/kg, i.p.) on blood ethanol concentration, hypothermia and handling-induced convulsions associated to acute ethanol administration were evaluated. The two-bottle choice paradigm was performed to assess the effects of CBD (30, 60 and 120 mg/kg/day, i.p.) on ethanol intake and preference. In addition, an oral ethanol self-administration experiment was carried out to evaluate the effects of CBD [a single s...
February 13, 2017: Addiction Biology
https://www.readbyqxmd.com/read/28191962/one-step-synthesis-of-hollow-dimpled-polystyrene-microparticles-by-dispersion-polymerization
#19
Sung Hwan Park, Jinwoo Kim, Wang-Eun Lee, Doo-Jin Byun, Mun Ho Kim
The design and preparation of hollow nonspherical microparticles are of great significance for their potential applications, but the development of a facile synthetic method using only one production step remains a great challenge. In the current work, a new template-free method based on dispersion polymerization was successfully developed to produce anisotropic hollow polystyrene (PS) microparticles in a single step. In the synthesis, ammonium persulfate (APS) played a critical role in the formation and growth of highly uniform and stable hollow PS microparticles...
February 17, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28190837/analysis-of-distribution-and-intraparticle-diffusion-of-a-fluorescent-dye-in-mesoporous-silica-gel-by-confocal-fluorescence-microspectroscopy
#20
Tatsumi Sato, Kiyoharu Nakatani
A micrometer-sized spherical silica gel microparticle (pore diameter, ∼7 nm) was injected into an aqueous rhodamine 6G solution using microcapillary manipulation-injection technique, and the dye distribution in the single microparticle was measured as the fluorescence depth profile by confocal fluorescence microspectroscopy. The fluorescence depth profile was simulated by the convolution and deconvolution methods to correct the contribution of the spatial resolution of the experimental system. The dye homogeneously or heterogeneously distributed in the microparticle at the adsorption equilibrium, dependent on the type of silica gel...
2017: Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry
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