keyword
MENU ▼
Read by QxMD icon Read
search

Megakaryocyte

keyword
https://www.readbyqxmd.com/read/28936487/platelet-dense-granules-begin-to-selectively-accumulate-mepacrine-during-proplatelet-formation
#1
Hayley A Hanby, Jialing Bao, Ji-Yoon Noh, Danuta Jarocha, Mortimer Poncz, Mitchell J Weiss, Michael S Marks
Platelet dense granules (DGs) are storage organelles for calcium ions, small organic molecules such as ADP and serotonin, and larger polyphosphates that are secreted upon platelet stimulation to enhance platelet activation, adhesion, and stabilization at sites of vascular damage. DGs are thought to fully mature within megakaryocytes (MKs) prior to platelet formation. Here we challenge this notion by exploiting vital fluorescent dyes to distinguish mildly acidic DGs from highly acidic compartments by microscopy in platelets and MKs...
August 22, 2017: Blood Advances
https://www.readbyqxmd.com/read/28935415/-hepatic-myelolipoma-a-rare-entity-case-report-and-review-of-the-literature
#2
Mathieu Gallo, Laurent Mineur, Hélène Emptas, Valérie Costes, Jeanne Ramos
Hepatic myelolipoma is a rare entity with only 17 cases described in the literature. A 73mm right liver mass was fortuitously discovered in a 55-year-old man. The biopsy showed normal hepatic tissue adjacent to a normal medular like hematopoïetic tissue, showing trilieage hematopoieses, including myeloid cells, erythroid cells and megakaryocytic cells. The diagnosis of hepatic myelolipoma was proposed. This benign tumor was initially described in adrenal gland, which is the most common topography. No malignancy or bleeding complication has been described in its hepatical location...
September 18, 2017: Annales de Pathologie
https://www.readbyqxmd.com/read/28931526/cellubrevin-vesicle-associated-membrane-protein-3-vamp-3-mediated-endocytosis-and-trafficking-regulate-platelet-functions
#3
Meenakshi Banerjee, Smita Joshi, Jinchao Zhang, Carole L Moncman, Shilpi Yadav, Beth A Bouchard, Brian Storrie, Sidney W Whiteheart
Endocytosis is key to fibrinogen (Fg) uptake, trafficking of integrins (αIIbβ3, αvβ3) and purinergic receptors (P2Y1, P2Y12), and thus for normal platelet function. However, the molecular machinery required and possible trafficking routes are still ill-defined. To further identify elements of the platelet endocytic machinery, we examined the role of a vesicle-residing, Soluble N-ethylmaleimide Factor Attachment Protein Receptor (v-SNARE) called Cellubrevin/Vesicle-Associated Membrane Protein-3 (VAMP-3) in platelet function...
September 20, 2017: Blood
https://www.readbyqxmd.com/read/28930689/integrating-enhancer-mechanisms-to-establish-a-hierarchical-blood-development-program
#4
Charu Mehta, Kirby D Johnson, Xin Gao, Irene M Ong, Koichi R Katsumura, Skye C McIver, Erik A Ranheim, Emery H Bresnick
Hematopoietic development requires the transcription factor GATA-2, and GATA-2 mutations cause diverse pathologies, including leukemia. GATA-2-regulated enhancers increase Gata2 expression in hematopoietic stem/progenitor cells and control hematopoiesis. The +9.5-kb enhancer activates transcription in endothelium and hematopoietic stem cells (HSCs), and its deletion abrogates HSC generation. The -77-kb enhancer activates transcription in myeloid progenitors, and its deletion impairs differentiation. Since +9...
September 19, 2017: Cell Reports
https://www.readbyqxmd.com/read/28928125/ck2%C3%AE-critically-regulates-thrombopoiesis-and-ca-2-triggered-platelet-activation-in-arterial-thrombosis-in-vivo
#5
Patrick Münzer, Britta Walker-Allgaier, Sascha Geue, Friederike Langhauser, Eva Geuss, David Stegner, Katja Aurbach, Daniela Semeniak, Madhumita Chatterjee, Irene Gonzalez Menendez, Melanie Märklin, Leticia Quintanilla-Martinez, Helmut R Salih, David W Litchfield, Thierry Buchou, Christoph Kleinschnitz, Florian Lang, Bernhard Nieswandt, Irina Pleines, Harald Schulze, Meinrad Gawaz, Oliver Borst
Platelets, anucleated megakaryocyte-derived cells, play a major role in hemostasis and arterial thrombosis. While protein kinase Casein Kinase 2 (CK2) is readily detected in megakaryocytes and platelets, the impact of CK2-dependent signaling on megakaryocyte/platelet (patho-) physiology has remained elusive. The present study explored the impact of the CK2 regulatory β-subunit on platelet biogenesis and activation. Megakaryocyte/platelet-specific genetic deletion of CK2β (ck2β(-/-) ) in mice resulted in a significant macrothrombocytopenia and an increased extramedullar megakaryopoiesis with enhanced proportion of premature platelets...
September 19, 2017: Blood
https://www.readbyqxmd.com/read/28914800/effect-of-clotting-duration-and-temperature-on-bdnf-measurement-in-human-serum
#6
Patrizia Amadio, Leonardo Sandrini, Alessandro Ieraci, Elena Tremoli, Silvia Stella Barbieri
Brain-derived neurothrophic factor (BDNF) is a neurotrophin expressed in different tissues and cells, including neurons, endothelial cells, leukocytes, megakaryocytes and platelets. Modifications of BDNF in plasma and/or in serum are associated with neurodegenerative and psychiatric disorders, cardiovascular diseases, metabolic syndrome and with mortality risk. Indeed, changes in blood levels of BDNF may reflect those of its tissue of origin and/or promote pathological dysfunctions. The measurement of BDNF amount in plasma or in serum has been characterized with particular attention in the impact of different anti-coagulants, clotting duration, temperature (≤21 °C) and delay in blood sample centrifugation as well as in stability of storage...
September 15, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28914425/platelet-factor-4-increases-bone-marrow-b-cell-development-and-differentiation
#7
David J Field, Angela A Aggrey-Amable, Sara K Blick, Sara K Ture, Andrew Johanson, Scott J Cameron, Sukanya Roy, Craig N Morrell
Platelet factor 4 (PF4) is a megakaryocyte-/platelet-derived chemokine with diverse functions as a regulator of vascular and immune biology. PF4 has a central role in vessel injury responses, innate immune cell responses, and T-helper cell differentiation. We have now discovered that PF4 has a direct role in B cell differentiation in the bone marrow. Mice lacking PF4 (PF4(-/-) mice) had fewer developing B cells in the bone marrow beginning after the pre-pro-B cell stage of differentiation. In vitro, PF4 increased the differentiation of hematopoietic progenitors to B cell lineage cells, indicating that PF4 has a direct effect on B cell differentiation...
September 15, 2017: Immunologic Research
https://www.readbyqxmd.com/read/28903944/a-dual-role-for-the-class-iii-pi3k-vps34-in-platelet-production-and-thrombus-growth
#8
Colin Valet, Marie Levade, Gaëtan Chicanne, Benoit Bilanges, Cendrine Cabou, Julien Viaud, Marie-Pierre Gratacap, Frédérique Gaits-Iacovoni, Bart Vanhaesebroeck, Bernard Payrastre, Sonia Severin
To uncover the role of Vps34, the sole class III phosphoinositide 3-kinase, in megakaryocytes (MKs) and platelets, we created a mouse model with Vps34 deletion in the MK/platelet lineage (Pf4-Cre/Vps34(lox/lox)). Deletion of Vps34 in MKs led to the loss of its regulator protein Vps15, and was associated with microthrombocytopenia and platelet granule abnormalities. While Vps34 deficiency did not impact on MK polyploidisation or proplatelet formation, it dampened MK granule biogenesis and directional migration towards an SDF1α gradient, leading to ectopic platelet release within the bone marrow...
September 13, 2017: Blood
https://www.readbyqxmd.com/read/28901537/tetrandrine-antagonizes-acute-megakaryoblastic-leukemia-growth-by-forcing-autophagy-mediated-differentiation
#9
Ting Liu, Zhenxing Zhang, Chunjie Yu, Chang Zeng, Xiaoqing Xu, Guixian Wu, Zan Huang, Wenhua Li
BACKGROUND AND PURPOSE: The dismal prognosis of acute megakaryoblastic leukemia (AMKL) urges for development of novel therapeutic methods. Inducing megakaryoblasts to undergo terminal differentiation was recently shown to be effective as a treatment for AMKL. This encouraged us to identify a potent anti-leukemia compound to induce megakaryocyte differentiation. EXPERIMENTAL APPROACH: Expression of CD41 and morphology change were observed in AMKL cells after tetrandrine treatment...
September 13, 2017: British Journal of Pharmacology
https://www.readbyqxmd.com/read/28898758/modular-flow-chamber-for-engineering-bone-marrow-architecture-and-function
#10
Christian A Di Buduo, Paolo M Soprano, Lorenzo Tozzi, Stefania Marconi, Ferdinando Auricchio, David L Kaplan, Alessandra Balduini
The bone marrow is a soft, spongy, gelatinous tissue found in the hollow cavities of flat and long bones that support hematopoiesis in order to maintain the physiologic turnover of all blood cells. Silk fibroin, derived from Bombyx mori silkworm cocoons, is a promising biomaterial for bone marrow engineering, because of its tunable architecture and mechanical properties, the capacity of incorporating labile compounds without loss of bioactivity and demonstrated ability to support blood cell formation. In this study, we developed a bone marrow scaffold consisting of a modular flow chamber made of polydimethylsiloxane, holding a silk sponge, prepared with salt leaching methods and functionalized with extracellular matrix components...
November 2017: Biomaterials
https://www.readbyqxmd.com/read/28887433/the-neurobeachin-like-2-protein-regulates-mast-cell-homeostasis
#11
Sebastian Drube, Randy Grimlowski, Carsten Deppermann, Julia Fröbel, Florian Kraft, Nico Andreas, David Stegner, Jan Dudeck, Franziska Weber, Mandy Rödiger, Christiane Göpfert, Julia Drube, Daniela Reich, Bernhard Nieswandt, Anne Dudeck, Thomas Kamradt
The neurobeachin-like 2 protein (Nbeal2) belongs to the family of beige and Chediak-Higashi (BEACH) domain proteins. Loss-of-function mutations in the human NBEAL2 gene or Nbeal2 deficiency in mice cause gray platelet syndrome, a bleeding disorder characterized by macrothrombocytopenia, splenomegaly, and paucity of α-granules in megakaryocytes and platelets. We found that in mast cells, Nbeal2 regulates the activation of the Shp1-STAT5 signaling axis and the composition of the c-Kit/STAT signalosome. Furthermore, Nbeal2 mediates granule formation and restricts the expression of the transcription factors, IRF8, GATA2, and MITF as well as of the cell-cycle inhibitor p27, which are essential for mast cell differentiation, proliferation, and cytokine production...
September 8, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28880435/thrombocytopenia-and-cd34-expression-is-decoupled-from-%C3%AE-granule-deficiency-with-mutation-of-the-first-gfi1b-zinc-finger
#12
D J Rabbolini, M-C Morel-Kopp, Q Chen, S Gabrielli, L C Dunlop, L P Chew, N Blair, T A Brighton, N Singh, A P Ng, C M Ward, W S Stevenson
BACKGROUND: Mutation of the GFI1B fifth DNA-binding zinc-finger domain causes macrothrombocytopenia and α-granule deficiency leading to clinical bleeding. The phenotypes associated with GFI1B variants disrupting non-DNA-binding zinc-fingers remain uncharacterized. OBJECTIVES: To determine the functional and phenotypic consequences of GFI1B variants disrupting non-DNA-binding zinc-finger domains. METHODS: The GFI1B C168F variant and a novel GFI1B c...
September 7, 2017: Journal of Thrombosis and Haemostasis: JTH
https://www.readbyqxmd.com/read/28876549/useful-laboratory-markers-for-the-diagnosis-of-bone-marrow-involvement-by-malignant-lymphoma
#13
K Nishimura, R Ota, Y Mikajiri, K Takahashi, S Sugishima, T Eto
INTRODUCTION: This study aimed to investigate the effect of bone marrow involvement by malignant lymphoma (BMI) on laboratory data and to determine the useful laboratory markers for diagnosing BMI. METHODS: We compared laboratory data between patients with and without BMI. We performed multivariate logistic regression and receiver operating characteristic (ROC) analyses to evaluate the diagnostic values of independent predictors. RESULTS: In the BMI group, platelets in peripheral blood (PLT) and megakaryocyte count in bone marrow (MgK) were significantly lower than those in the non-BMI group (PLT, P < ...
September 6, 2017: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/28872489/a-case-of-pure-red-cell-aplasia-during-nivolumab-therapy-for-cardiac-metastatic-melanoma
#14
Akihiko Yuki, Tatsuya Takenouchi, Sumiko Takatsuka, Takuro Ishiguro
Nivolumab is an antibody against programmed cell death 1 and functions as an immune checkpoint inhibitor for various malignancies, including unresectable melanomas. Nivolumab causes several immune-related adverse events, which typically include skin rash, pneumonitis, thyroid dysfunction, hepatitis, and colitis; in rare cases, anemia may be present. There are several reports of autoimmune hemolytic anemia that has developed in response to nivolumab; however, there are few reports of pure red cell aplasia (PRCA)...
September 1, 2017: Melanoma Research
https://www.readbyqxmd.com/read/28867167/pediatric-acute-megakaryoblastic-leukemia-multitasking-fusion-proteins-and-oncogenic-cooperations
#15
REVIEW
Cécile K Lopez, Sébastien Malinge, Muriel Gaudry, Olivier A Bernard, Thomas Mercher
Pediatric leukemia presents specific clinical and genetic features from adult leukemia but the underpinning mechanisms of transformation are still unclear. Acute megakaryoblastic leukemia (AMKL) is the malignant accumulation of progenitors of the megakaryocyte lineage that normally produce blood platelets. AMKL is diagnosed de novo, in patients showing a poor prognosis, or in Down syndrome (DS) patients with a better prognosis. Recent data show that de novo AMKL is primarily associated with chromosomal alterations leading to the expression of fusions between transcriptional regulators...
September 2017: Trends in Cancer
https://www.readbyqxmd.com/read/28866671/autoimmune-myelofibrosis-clinical-features-course-and-outcome
#16
Caroline I Piatek, Maria E Vergara-Lluri, Vinod Pullarkat, Imran N Siddiqi, Casey O'Connell, Russell K Brynes, Donald I Feinstein
BACKGROUND: Autoimmune myelofibrosis (AIMF) is an underrecognized cause of nonmalignant bone marrow fibrosis which occurs in the presence or absence of a defined systemic autoimmune disease. Patients with AIMF present with cytopenias and autoantibodies, and have a distinctive nonclonal myelofibrosis on bone marrow examination. AIMF is distinguished from primary myelofibrosis by the absence of splenomegaly, eosinophilia, or basophilia, and the absence of abnormal myeloid, erythroid, or megakaryocytic morphology...
September 2, 2017: Acta Haematologica
https://www.readbyqxmd.com/read/28859041/case-report-clinical-variation-in-children-with-thrombopoietin-receptor-c-mpl-mutations-report-of-2-cases
#17
Clara Lo, Elysia Alvarez, Robert S Ohgami, Michael Jeng
Congenital amegakaryocytic thrombocytopenia (CAMT, MIM# 604498) is a rare congenital bone marrow failure syndrome which presents early in life with abnormal bleeding because of thrombocytopenia. Classically, megakaryocytes are decreased to absent in the bone marrow. The development of aplastic anemia early in childhood has led to the recommendation for early stem cell transplantation. Quantitative or loss-of-function mutations in the myeloproliferative leukemia gene (c-mpl), whose gene product functions as the thrombopoietin receptor, have been identified as causative for CAMT...
August 30, 2017: Journal of Pediatric Hematology/oncology
https://www.readbyqxmd.com/read/28854375/deletion-of-glut1-and-glut3-reveals-multiple-roles-for-glucose-metabolism-in-platelet-and-megakaryocyte-function
#18
Trevor P Fidler, Robert A Campbell, Trevor Funari, Nicholas Dunne, Enrique Balderas Angeles, Elizabeth A Middleton, Dipayan Chaudhuri, Andrew S Weyrch, E Dale Abel
No abstract text is available yet for this article.
August 29, 2017: Cell Reports
https://www.readbyqxmd.com/read/28834165/a-gain-of-function-mutation-in-tnfrsf13b-is-a-candidate-for-predisposition-to-familial-or-sporadic-immune-thrombocytopenia
#19
Hong-Ling Peng, Yang Zhang, Nan-Nan Sun, Ya-Fei Yin, Ye-Wei Wang, Zhao Cheng, Wen-Zhe Yan, Su-Fang Liu, Yun-Xiao Xu, Xiang Xiao, Guang-Sen Zhang
BACKGROUND: Most immune thrombocytopenia (ITP) is sporadic but a positive family history of ITP in some patients suggests that hereditary forms exist. Due to the rarity of familial ITP families available for study and the heterogeneity of sporadic ITP, family linkage analysis or genome wide association studies are limited. OBJECTIVES: Based on one ITP pedigree, we try to identify the predisposing gene in familial or sporadic ITP and reveal the way in which it causes thrombocytopenia...
August 20, 2017: Journal of Thrombosis and Haemostasis: JTH
https://www.readbyqxmd.com/read/28832006/megakaryocytic-emperipolesis-associated-with-thrombocytopenia-causative-or-coincidence
#20
Manu Goyal, Sreeja Thandilath Thekkelakayil, Anurag Gupta
No abstract text is available yet for this article.
August 23, 2017: Turkish Journal of Haematology: Official Journal of Turkish Society of Haematology
keyword
keyword
31957
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"