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refractory periapical periodontitis

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https://www.readbyqxmd.com/read/27706660/preliminary-study-on-total-protein-extraction-methods-from-enterococcus-faecalis-biofilm
#1
W Chen, J Liang, Z He, W Jiang
Enterococcus faecalis is the major pathogen of post-endodontic disease and refractory periapical periodontitis, and recent research on this species has focused on its pathogenicity. E. faecalis most often causes disease in the form of a biofilm, and total protein expression shows a strong association with its virulence. Therefore, the purpose of our study was to explore different methods of extracting the total proteins of the E. faecalis (ATCC 33186 standard strain) biofilm. The total proteins in the biofilm were extracted using an ultrasonication method with varied parameters, including duration, amplitude setting, period, and duty cycle...
August 30, 2016: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/25649294/enterococcus-faecalis-attenuates-the-differentiation-of-macrophages-into-osteoclasts
#2
Ok-Jin Park, Jihyun Yang, Jiseon Kim, Cheol-Heui Yun, Seung Hyun Han
INTRODUCTION: Enterococcus faecalis is closely associated with refractory apical periodontitis, manifesting periapical lesions and alveolar bone loss. Macrophages playing an important role in the induction of inflammation can differentiate into bone-resorbing osteoclasts. In the present study, we investigated the effect of E. faecalis on the differentiation and function of macrophages as osteoclast precursors. METHODS: Bone marrow-derived macrophages (BMMs) were differentiated into osteoclasts with macrophage colony-stimulating factor and receptor activator of nuclear factor kappa B ligand in the presence or absence of heat-killed E...
May 2015: Journal of Endodontics
https://www.readbyqxmd.com/read/24747900/promotion-of-endodontic-lesions-in-rats-by-a-novel-extraradicular-biofilm-model-using-obturation-materials
#3
Katsutaka Kuremoto, Yuichiro Noiri, Takuya Ishimoto, Naomichi Yoneda, Reiko Yamamoto, Hazuki Maezono, Takayoshi Nakano, Mikako Hayashi, Shigeyuki Ebisu
Although extraradicular biofilm formation is related to refractory periapical periodontitis, the mechanism of extraradicular biofilm development, as well as its effect on periapical lesions, is unknown. Therefore, we aimed to develop an in vivo extraradicular biofilm model in rats and to identify and quantify extraradicular biofilm-forming bacteria while investigating the effect of extraradicular biofilms on periapical lesions. Periapical lesions were induced by exposing the pulpal tissue of the mandibular first molars of male Wistar rats to their oral environment...
July 2014: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/23611383/outcome-of-periapical-lesions-in-a-rat-model-of-type-2-diabetes-refractoriness-to-systemic-antioxidant-therapy
#4
Carlos Frederico B Wolle, Luciana A Zollmann, Patrícia O Bairros, Adriana Etges, Carlos E Leite, Fernanda B Morrone, Maria M Campos
INTRODUCTION: This study evaluated the development of periapical lesions in a rat model of type 2 diabetes and assessed the potential actions of the antioxidant agent tempol in this model. METHODS: Male Wistar rats were used; they received tap water (N = 5) or a 20% glucose solution (N = 15) during a period of 9 weeks. At the sixth week, periapical lesions were induced on the first mandibular molars, and the animals were subdivided into 4 groups. The subgroup 1 was composed of nondiabetic rats orally receiving saline solution (10 mL/kg)...
May 2013: Journal of Endodontics
https://www.readbyqxmd.com/read/23268602/case-report-uncommon-maxillary-lateral-incisor-with-a-type-3-invagination-and-c-shaped-root-canal-system
#5
Ronald Riley, James Dryden
Dental practitioners are often called upon to provide endodontic services for patients in pain. Maxillary lateral incisors may present added concerns when planning endodontic services. Dens invaginatus (DI) Oehlers Type 3 classification is a relatively rare phenomenon that occurs primarily in maxillary lateral incisors. It is important to consider this condition in all maxillary lateral incisors and to be aware that it can be difficult to treat when the invagination extends past the epithelial attachment apparatus...
June 2012: Compendium of Continuing Education in Dentistry
https://www.readbyqxmd.com/read/22507081/bactericidal-activity-and-oral-pathogen-inactivation-by-electromagnetic-wave-irradiation
#6
H Yumoto, T Tominaga, K Hirao, T Kimura, K Takahashi, T Sumitomo, N Bando, T Matsuo
AIMS:   The aim of this work was to clarify the effects of electromagnetic wave irradiation (EMWI) on oral bacterial pathogens. METHODS AND RESULTS:   A Gram-negative (Porphyromonas gingivalis) or Gram-positive (Streptococcus mutans, S. intermedius, Enterococcus faecalis) bacterial suspension was irradiated by EMW apparatus (500-1000 kHz, 5-15 times, 1 s time(-1) ). Quantification of survival bacteria by CFU counting revealed that EMWI exhibited marked bactericidal activity against all tested bacteria and bactericidal activity at 500 kHz increased in an irradiation number-dependent manner...
July 2012: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/22041013/root-canal-treatment-of-an-immature-dens-invaginatus-with-apical-periodontitis-a-case-report
#7
Francisco Wanderley Garcia Paula-Silva, Cristiane Tomaz Rocha, Daniel Silva Herzog Flores, Paulo Nelson-Filho, Léa Assed Bezerra da Silva, Alexandra Mussolino de Queiroz
The purpose of this paper was to describe the case of an 11-year-old patient who presented a dens invaginatus, detected in the permanent maxillary left lateral incisor, with an immature open apex and extensive apical periodontitis and sinus tract. The mineralized invaginated barrier was removed, and a nonsurgical root canal treatment was performed using both calcium hydroxide as a root canal dressing to stimulate apexification and a mineral trioxide aggregate (MTA) plug to permit root canal filling. After a 14-month period of root canal dressing changes, no evidence of apical periodontitis was observed, and the MTA plug was placed in the root canal's apical portion...
January 2011: Journal of Dentistry for Children
https://www.readbyqxmd.com/read/21731719/por-secretion-system-dependent-secretion-and-glycosylation-of-porphyromonas-gingivalis-hemin-binding-protein-35
#8
Mikio Shoji, Keiko Sato, Hideharu Yukitake, Yoshio Kondo, Yuka Narita, Tomoko Kadowaki, Mariko Naito, Koji Nakayama
The anaerobic Gram-negative bacterium Porphyromonas gingivalis is a major pathogen in severe forms of periodontal disease and refractory periapical perodontitis. We have recently found that P. gingivalis has a novel secretion system named the Por secretion system (PorSS), which is responsible for secretion of major extracellular proteinases, Arg-gingipains (Rgps) and Lys-gingipain. These proteinases contain conserved C-terminal domains (CTDs) in their C-termini. Hemin-binding protein 35 (HBP35), which is one of the outer membrane proteins of P...
2011: PloS One
https://www.readbyqxmd.com/read/21155997/enterococcus-faecalis-infection-in-root-canals-host-derived-or-exogenous-source
#9
R Vidana, A Sullivan, H Billström, M Ahlquist, B Lund
AIMS: Enterococcus faecalis is associated with a significant number of refractory endodontic infections. Previous studies report a prevalence of Ent. faecalis ranging from 24% up to 77% in teeth with failed endodontic treatment. The origin of the micro-organism remains unclear, as enterococci do not belong to the normal oral microflora. The aim of this study was to determine whether these enterococci were of endogenous or exogenous origin. METHODS AND RESULTS: Fifty consecutive patients with apical periodontitis in need of endodontic orthograde re-treatment were included...
February 2011: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/20610294/surgical-endodontic-treatment-of-refractory-periapical-periodontitis-with-extraradicular-biofilm
#10
Lingyun Su, Yang Gao, Chengjun Yu, Hanguo Wang, Qing Yu
OBJECTIVE: This study explored the effect of surgical endodontic treatment of refractory periapical periodontitis with extraradicular biofilm. STUDY DESIGN: Endodontic surgery was conducted on a case of refractory periapical periodontitis with extraradicular biofilm. During the procedure, the samples of periapical tissues were collected for microbiologic analysis. The resected root ends were observed under scanning electron microscope. The patient was recalled every 3 months to evaluate the treatment effect for 1 year...
July 2010: Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics
https://www.readbyqxmd.com/read/20368043/-etiology-and-treatment-strategy-of-refractory-periapical-periodontitis
#11
Jun-qi Ling, Xi Wei, Hong-yan Liu
No abstract text is available yet for this article.
January 2010: Zhonghua Kou Qiang Yi Xue za Zhi, Zhonghua Kouqiang Yixue Zazhi, Chinese Journal of Stomatology
https://www.readbyqxmd.com/read/19127823/-biofilm-formation-on-root-canal-review
#12
REVIEW
Paulina Debicka, Mariusz Lipski, Jadwiga Buczkowska-Radlińska, Matylda Trusewicz
It seems likely that one of the reasons for failures in the endodontic treatment is the presence of biofilm in root canals. Biofilm bacteria have a slower metabolism and higher resistance and virulence due to phenotypic changes. The occurrence of biofilms has been reported both inside the canal and on the external root surface. The results of many studies suggest that biofilm may be associated with refractory periapical periodontitis and is often caused by the coronal leakage.
2008: Annales Academiae Medicae Stetinensis
https://www.readbyqxmd.com/read/18634930/lipoteichoic-acid-partially-contributes-to-the-inflammatory-responses-to-enterococcus-faecalis
#13
Jung Eun Baik, Young Hee Ryu, Ji Young Han, Jintaek Im, Kee-Yeon Kum, Cheol-Heui Yun, Kangseok Lee, Seung Hyun Han
Enterococcus faecalis, a pathogenic gram-positive bacterium, is closely related to refractory apical periodontitis. Because lipoteichoic acid (LTA) is considered a major virulence factor of gram-positive bacteria, in the present study, highly pure LTA from E. faecalis was prepared, and its ability to stimulate murine macrophages was investigated in comparison with those of the killed whole cells. Upon exposure to E. faecalis LTA, RAW 264.7 (a murine macrophage cell line) produced a significantly (p < 0.05) high level of tumor necrosis factor-alpha (TNF-alpha) and nitric oxide (NO) in a concentration-dependent manner...
August 2008: Journal of Endodontics
https://www.readbyqxmd.com/read/18363699/ridge-preservation-in-cases-requiring-tooth-extraction-during-endodontic-surgery-a-case-report
#14
S Lin, N Cohenca, E A Muska, E Front
AIM: To provide a treatment option to endodontists performing surgery that will enhance ridge preservation when tooth extraction is required. SUMMARY: A 47-year-old woman was referred to an endodontic practice for apical root-end resection of tooth 22 because of refractory periradicular disease. Radiographic examination revealed a large periradicular lesion, 5 mm in diameter and a root-end filling at the root apex. The treatment plan included exploratory surgery and apical root-end resection...
May 2008: International Endodontic Journal
https://www.readbyqxmd.com/read/16332869/identification-and-localization-of-extraradicular-biofilm-forming-bacteria-associated-with-refractory-endodontic-pathogens
#15
Nobuo Noguchi, Yuichiro Noiri, Masahiro Narimatsu, Shigeyuki Ebisu
Bacterial biofilms have been found to develop on root surfaces outside the apical foramen and be associated with refractory periapical periodontitis. However, it is unknown which bacterial species form extraradicular biofilms. The present study aimed to investigate the identity and localization of bacteria in human extraradicular biofilms. Twenty extraradicular biofilms, used to identify bacteria using a PCR-based 16S rRNA gene assay, and seven root-tips, used to observe immunohistochemical localization of three selected bacterial species, were taken from 27 patients with refractory periapical periodontitis...
December 2005: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/15810977/calculus-like-deposit-on-the-apical-external-root-surface-of-teeth-with-post-treatment-apical-periodontitis-report-of-two-cases
#16
D Ricucci, M Martorano, A L Bate, E A Pascon
AIM: To report two cases in which calculus-like material was found on external root surfaces of (i) an extracted root and (ii) an apicected part of a root, both of which were removed due to post-treatment refractory apical periodontitis. SUMMARY: In each case, there was a fistulous tract, which did not heal after conventional root canal treatment. The first case did not heal even after apical surgery, and subsequent tooth extraction revealed calculus-like material on a root surface of complex anatomy...
April 2005: International Endodontic Journal
https://www.readbyqxmd.com/read/15759963/cutaneous-draining-sinus-tract-of-odontogenic-origin-unusual-presentation-of-a-challenging-diagnosis
#17
Daniel J Sheehan, Brad J Potter, Loretta S Davis
A 44-year-old woman presented with a chronically draining lesion on her cheek just lateral to the nasofacial sulcus. The lesion was refractory to treatment with oral antibiotics. Physical examination revealed poor dentition, and a panoramic radiograph demonstrated periapical abscesses in the maxillary right lateral incisor and canine. A diagnosis of cutaneous fistula of odontogenic origin was made, and the patient was treated with tooth extraction. The cutaneous fistula subsequently resolved. Intraoral examinations and radiographs are critical for making the diagnosis of cutaneous draining sinus tract of odontogenic origin...
February 2005: Southern Medical Journal
https://www.readbyqxmd.com/read/15560836/single-species-biofilm-forming-ability-of-root-canal-isolates-on-gutta-percha-points
#18
Naoki Takemura, Yuichiro Noiri, Atsushi Ehara, Takashi Kawahara, Nobuo Noguchi, Shigeyuki Ebisu
The participation of bacterial biofilms in the over-filled gutta-percha points associated with refractory periapical periodontitis has recently been reported. This study investigated the initial biofilm-forming ability of root canal isolates (Enterococcus faecalis, Streptococcus sanguis, Strep. intermedius, Strep. pyogenes, Staphylococcus aureus, Fusobacterium nucleatum, Propionibacterium acnes, Porphyromonas gingivalis and Prevotella intermedia) on gutta-percha points in vitro. Each bacterial strain was suspended in 100% cell culture medium or in culture medium containing 4...
December 2004: European Journal of Oral Sciences
https://www.readbyqxmd.com/read/15139959/intentional-replantation-of-an-immature-permanent-lower-incisor-because-of-a-refractory-peri-apical-lesion-case-report-and-5-year-follow-up
#19
S Shintani, M Tsuji, S Toyosawa, T Ooshima
We performed an intentional replantation of an immature lower incisor that had a refractory peri-apical lesion. The incisor was extracted and the peri-apical lesion was removed by curettage. The root canal of the tooth was then rapidly irrigated, and filled with a calcium hydroxide and iodoform paste (Vitapex(R)), after which the tooth was fixed with an arch wire splint. Five years later, no clinical or radiographic abnormalities were found, and the root apex was obturated by an apical bridge formation. A team of two dentists is essential to prevent a prolonged operation time, thus eliminating any of the causes of ankylosis...
May 2004: International Journal of Paediatric Dentistry
https://www.readbyqxmd.com/read/12398163/participation-of-bacterial-biofilms-in-refractory-and-chronic-periapical-periodontitis
#20
Yuichiro Noiri, Atsushi Ehara, Takashi Kawahara, Naoki Takemura, Shigeyuki Ebisu
The aim of this study was to examine morphologically the participation of extraradicular biofilm in refractory periapical periodontitis. Six teeth and five extruded root filling gutta-percha points associated with refractory periapical periodontitis were investigated by scanning electron microscope. In nine of 11 samples examined, bacterial biofilms were seen at the extraradicular area. The gutta-percha surface was covered with glycocalyx-like structures, and filaments, long rods, and spirochete-shaped bacteria were predominant in the extraradicular sites...
October 2002: Journal of Endodontics
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