keyword
https://read.qxmd.com/read/38536913/cardiac-tissue-model-of-immune-induced-dysfunction-reveals-the-role-of-free-mitochondrial-dna-and-the-therapeutic-effects-of-exosomes
#1
JOURNAL ARTICLE
Rick Xing Ze Lu, Naimeh Rafatian, Yimu Zhao, Karl T Wagner, Erika L Beroncal, Bo Li, Carol Lee, Jingan Chen, Eryn Churcher, Daniel Vosoughi, Chuan Liu, Ying Wang, Andrew Baker, Uriel Trahtemberg, Bowen Li, Agostino Pierro, Ana C Andreazza, Claudia C Dos Santos, Milica Radisic
Despite tremendous progress in the development of mature heart-on-a-chip models, human cell-based models of myocardial inflammation are lacking. Here, we bioengineered a vascularized heart-on-a-chip with circulating immune cells to model severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-induced acute myocarditis. We observed hallmarks of coronavirus disease (COVID-19)-induced myocardial inflammation, as the presence of immune cells augmented the secretion of proinflammatory cytokines, triggered progressive impairment of contractile function, and altered intracellular calcium transients...
March 29, 2024: Science Advances
https://read.qxmd.com/read/38536871/species-specific-responses-during-seoul-orthohantavirus-infection-in-human-and-rat-lung-microvascular-endothelial-cells
#2
JOURNAL ARTICLE
Danny Noack, Mirjam C G N van den Hout, Carmen W E Embregts, Wilfred F J van IJcken, Marion P G Koopmans, Barry Rockx
Seoul orthohantavirus (SEOV) is a rat-borne zoonotic virus that is transmitted via inhalation of aerosolized infectious excreta, and can cause hemorrhagic fever with renal syndrome (HFRS) in humans worldwide. In rats, SEOV predominantly exists as a persistent infection in the absence of overt clinical signs. Lack of disease in rats is attributed to downregulation of pro-inflammatory and upregulation of regulatory host responses. As lung microvascular endothelial cells (LMECs) represent a primary target of infection in both human and rats, infections in these cells provide a unique opportunity to study the central role of LMECs in the dichotomy between pathogenicity in both species...
March 27, 2024: PLoS Neglected Tropical Diseases
https://read.qxmd.com/read/38536748/defining-t-cell-receptor-repertoires-using-nanovial-based-binding-and-functional-screening
#3
JOURNAL ARTICLE
Doyeon Koo, Zhiyuan Mao, Robert Dimatteo, Miyako Noguchi, Natalie Tsubamoto, Jami McLaughlin, Wendy Tran, Sohyung Lee, Donghui Cheng, Joseph de Rutte, Giselle Burton Sojo, Owen N Witte, Dino Di Carlo
The ability to selectively bind to antigenic peptides and secrete effector molecules can define rare and low-affinity populations of cells with therapeutic potential in emerging T cell receptor (TCR) immunotherapies. We leverage cavity-containing hydrogel microparticles, called nanovials, each coated with peptide-major histocompatibility complex (pMHC) monomers to isolate antigen-reactive T cells. T cells are captured and activated by pMHCs inducing the secretion of effector molecules including IFN-γ and granzyme B that are accumulated on nanovials, allowing sorting based on both binding and function...
April 2, 2024: Proceedings of the National Academy of Sciences of the United States of America
https://read.qxmd.com/read/38536662/natural-killer-group%C3%A2-2d-major-histocompatibility-complex-class%C3%A2-i-polypeptide-related-sequence%C3%A2-a-activation-enhances-natural-killer-cell-mediated-immunity-against-hepatocellular-carcinoma-a-review
#4
REVIEW
Jun Arai, Akinori Okumura, Naoya Kato, Kiyoaki Ito
The recent clinical introduction of immune checkpoint inhibitors has improved therapeutic outcomes in patients with advanced hepatocellular carcinoma. However, these therapies targeting CD8+ T lymphocytes have a response rate of approximately 30%. In addition to CD8+ T lymphocytes, natural killer (NK) cells represent promising therapeutic targets for hepatocellular carcinoma, because they comprise 30%-50% of all lymphocytes in the liver and contribute to antitumor immunity. A recent meta-analysis revealed that the percentage of infiltrating NK cells in hepatocellular carcinoma correlates with a better patient outcome...
March 27, 2024: Hepatology Research: the Official Journal of the Japan Society of Hepatology
https://read.qxmd.com/read/38536645/recent-advances-in-understanding-the-biology-of-follicular-lymphoma
#5
REVIEW
Momoko Nishikori
Follicular lymphoma (FL), the most common indolent B-cell lymphoma, develops over decades before manifesting as overt disease. BCL2 overexpression by t(14;18) confers a survival advantage to B cells during the germinal center reaction, and abnormalities in epigenetic modifier genes lead to desynchronization of gene expression changes in germinal center B cells. Studies in mouse models have shown that BCL2 overexpression and epigenetic deregulation in B cells cooperatively promote lymphomagenesis. The immune microenvironment also plays an essential role in the biology of FL, and many molecular prognostic indicators based on the immune microenvironment have been proposed...
March 27, 2024: International Journal of Hematology
https://read.qxmd.com/read/38536635/the-acetic-acid-produced-by-lactobacillus-species-regulates-immune-function-to-alleviate-pedv-infection-in-piglets
#6
JOURNAL ARTICLE
Ming-Jie Sun, Jun Hong Xing, Qing-Song Yan, Bo-Shi Zou, Ying-Jie Wang, Tian-Ming Niu, Tong Yu, Hai-Bin Huang, Di Zhang, Shu-Min Zhang, Wu-Sheng Sun, Ruo-Nan Zou, Chun-Feng Wang, Chun-Wei Shi
Porcine epidemic diarrhea virus (PEDV) infection results in significant mortality among newborn piglets, leading to substantial economic setbacks in the pig industry. Short-chain fatty acids (SCFA), the metabolites of intestinal probiotics, play pivotal roles in modulating intestinal function, enhancing the intestinal barrier, and bolstering immune responses through diverse mechanisms. The protective potential of Lactobacillus delbrueckii, Lactobacillus johnsonii, and Lactococcus lactis was first noted when administered to PEDV-infected piglets...
March 27, 2024: Probiotics and Antimicrobial Proteins
https://read.qxmd.com/read/38536531/minimally-invasive-approaches-to-diagnose-and-monitor-eosinophilic-gi-diseases
#7
REVIEW
Ellie J M Furuta, Glenn T Furuta, Robin Shandas
PURPOSE OF REVIEW: This review seeks to understand novel avenues for eosinophilic GI disease management. Biomarkers offer a unique and non-invasive approach to tracking EoE disease progression. While no biomarkers have definitively met the diagnostic criteria for eosinophilic GI diseases, some biomarkers have been shown to be associated with disease activity. Here, we examine the potential of recently studied biomarkers. RECENT FINDINGS: Current research shows advancements in blood, luminal fluid, and breath testing...
March 27, 2024: Current Allergy and Asthma Reports
https://read.qxmd.com/read/38536445/after-wounding-a-g-protein-coupled-receptor-promotes-the-restoration-of-tension-in-epithelial-cells
#8
JOURNAL ARTICLE
Ivy Han, Junmin Hua, James S White, James T O'Connor, Lila S Nassar, Kaden J Tro, Andrea Page-McCaw, M Shane Hutson
The maintenance of epithelial barrier function involves cellular tension, with cells pulling on their neighbors to maintain epithelial integrity. Wounding interrupts cellular tension, which may serve as an early signal to initiate epithelial repair. To characterize how wounds alter cellular tension, we used a laser-recoil assay to map cortical tension around wounds in the epithelial monolayer of the Drosophila pupal notum.  Within a minute of wounding, there was widespread loss of cortical tension along both radial and tangential directions...
March 27, 2024: Molecular Biology of the Cell
https://read.qxmd.com/read/38536129/the-deep-learning-framework-icantcr-enables-early-cancer-detection-using-the-t-cell-receptor-repertoire-in-peripheral-blood
#9
JOURNAL ARTICLE
Yideng Cai, Meng Luo, Wenyi Yang, Chang Xu, Pingping Wang, Guangfu Xue, Xiyun Jin, Rui Cheng, Jinhao Que, Wenyang Zhou, Boran Pang, Shouping Xu, Yu Li, Qinghua Jiang, Zhaochun Xu
T cells recognize tumor antigens and initiate an anti-cancer immune response in the very early stages of tumor development, and the antigen specificity of T cells is determined by the T cell receptor (TCR). Therefore, monitoring changes in the TCR repertoire in peripheral blood may offer a strategy to detect various cancers at a relatively early stages. Here, we developed the deep learning framework iCanTCR to identify cancer patients based on the TCR repertoire. The iCanTCR framework uses TCRβ sequences from an individual as an input and outputs the predicted cancer probability...
March 27, 2024: Cancer Research
https://read.qxmd.com/read/38536023/tim3-and-ctla4-immune-checkpoint-polymorphisms-are-associated-with-acute-myeloid-leukemia-in-saudi-arabia
#10
JOURNAL ARTICLE
Mashael Alqahtani, Ali Aljuaimlani, Jameel Al-Tamimi, Suliman Alomar, Lamjed Mansour
BACKGROUND: Immune checkpoints are receptors on the surface of T cells that function crucially in suppressing the immune response, and they are implicated in autoimmunity and cancer diseases. AIM: The present study aimed to investigate the relationship between functional single nucleotide polymorphisms (SNPs) of two immune checkpoint molecules, CTLA-4 and TIM-3, and acute myeloid leukemia (AML) in a Saudi population. METHODS: Two SNPs in CTLA-4 (rs231775, A > G) and TIM-3 (rs10515746, A > C) were genotyped in 229 subjects, including 98 patients and 131 healthy controls, from the Saudi population using TaqMan assay methods...
December 2024: Hematology (Amsterdam, Netherlands)
https://read.qxmd.com/read/38535478/effect-of-ishige-okamurae-extract-on-osteoclastogenesis-in-vitro-and-in-vivo
#11
JOURNAL ARTICLE
Su-Hyeon Cho, Hyun-Soo Kim, Juhee Ahn, Bomi Ryu, Jun-Geon Jea, Kyubin Lee, Kyunghwan Kim, Ginnae Ahn, WonWoo Lee, Kyung-Min Choi, Kil-Nam Kim
We demonstrated the effect of Ishige okamurae extract (IOE) on the receptor activator of nuclear factor-κB ligand (RANKL)-promoted osteoclastogenesis in RAW 264.7 cells and confirmed that IOE inhibited RANKL-induced tartrate-resistant acid phosphatase (TRAP) activity and osteoclast differentiation. IOE inhibited protein expression of TRAP, metallopeptidase-9 (MMP-9), the calcitonin receptor (CTR), and cathepsin K (CTK). IOE treatment suppressed the expression of activated T cell cytoplasmic 1 and activator protein-1, thus controlling the expression of osteoclast-related factors...
March 20, 2024: Marine Drugs
https://read.qxmd.com/read/38535258/in-vitro-effects-of-weissella-cibaria-cmu-and-cms1-on-receptor-activator-of-nf-%C3%AE%C2%BAb-ligand-rankl-induced-osteoclast-differentiation
#12
JOURNAL ARTICLE
Geun-Yeong Park, Jeong-Ae Park, Mi-Sun Kang
Excessive osteoclast activity can promote periodontitis-associated bone destruction. The inhibitory mechanisms of Weissella cibaria strains CMU and CMS1 against periodontitis have not yet been fully elucidated. In this study, we aimed to investigate whether heat-killed (HK) W. cibaria CMU and CMS1 or their respective cell-free supernatants (CFSs) inhibit osteoclast differentiation and bone resorption in response to receptor activator of nuclear factor kappa-B ligand (RANKL)-treated RAW 264.7 cells. TRAP (tartrate-resistant acid phosphatase) staining and bone resorption assays revealed that both HK bacteria and CFSs significantly suppressed the number of TRAP-positive cells, TRAP activity, and bone pit formation compared to the RANKL-treated control ( p < 0...
March 8, 2024: Journal of Functional Biomaterials
https://read.qxmd.com/read/38534788/the-opposite-functions-of-cd30-ligand-isoforms
#13
JOURNAL ARTICLE
Ignat Printsev, Elyas Alalli, Janine Bilsborough
TNFSF8/CD30 ligand is a TNF superfamily member expressed on several major immune cell types, including activated monocytes, B, and T cells. The signaling of CD30 ligand through its cognate CD30 receptor has been shown to have effects on cell differentiation, cell death/survival, and cytokine production. The signaling pair has been implicated in hematopoietic malignancies and inflammatory disease, and a chemotherapy-CD30 antibody combination for the treatment of Hodgkin and other lymphomas has been developed...
March 21, 2024: Current Issues in Molecular Biology
https://read.qxmd.com/read/38534772/5-aza-upregulates-socs3-and-ptpn6-shp1-inhibiting-stat3-and-potentiating-the-effects-of-ag490-against-primary-effusion-lymphoma-cells
#14
JOURNAL ARTICLE
Michele Di Crosta, Andrea Arena, Rossella Benedetti, Maria Saveria Gilardini Montani, Mara Cirone
Epigenetic modifications, including aberrant DNA methylation occurring at the promoters of oncogenes and oncosuppressor genes and histone modifications, can contribute to carcinogenesis. Aberrant methylation mediated by histone methylatransferases, alongside histones, can affect methylation of proteins involved in the regulation of pro-survival pathways such as JAK/STAT and contribute to their activation. In this study, we used DNA or histone demethylating agents, 5-Azacytidine (5-AZA) or DS-3201 (valemetostat), respectively, to treat primary effusion lymphoma (PEL) cells, alone or in combination with AG490, a Signal transducer and activator of transcription 3 (STAT3) inhibitor...
March 14, 2024: Current Issues in Molecular Biology
https://read.qxmd.com/read/38534465/engineering-a-dual-specificity-%C3%AE-%C3%AE-t-cell-receptor-for-cancer-immunotherapy
#15
JOURNAL ARTICLE
David M Davies, Giuseppe Pugliese, Ana C Parente Pereira, Lynsey M Whilding, Daniel Larcombe-Young, John Maher
γδ T-cells provide immune surveillance against cancer, straddling both innate and adaptive immunity. G115 is a clonal γδ T-cell receptor (TCR) of the Vγ9Vδ2 subtype which can confer responsiveness to phosphoantigens (PAgs) when genetically introduced into conventional αβ T-cells. Cancer immunotherapy using γδ TCR-engineered T-cells is currently under clinical evaluation. In this study, we sought to broaden the cancer specificity of the G115 γδ TCR by insertion of a tumour-binding peptide into the complementarity-determining region (CDR) three regions of the TCR δ2 chain...
March 20, 2024: Biology
https://read.qxmd.com/read/38534424/the-impact-of-cytomegalovirus-infection-on-natural-killer-and-cd8-t-cell-phenotype-in-multiple-sclerosis
#16
JOURNAL ARTICLE
Valentina Perri, Maria Antonella Zingaropoli, Patrizia Pasculli, Federica Ciccone, Matteo Tartaglia, Viola Baione, Leonardo Malimpensa, Gina Ferrazzano, Claudio Maria Mastroianni, Antonella Conte, Maria Rosa Ciardi
Multiple sclerosis (MS) is a debilitating neurological disease that has been classified as an immune-mediated attack on myelin, the protective sheath of nerves. Some aspects of its pathogenesis are still unclear; nevertheless, it is generally established that viral infections influence the course of the disease. Cytomegalovirus (CMV) is a major pathogen involved in alterations of the immune system, including the expansion of highly differentiated cytotoxic CD8+ T cells and the accumulation of adaptive natural killer (NK) cells expressing high levels of the NKG2C receptor...
February 28, 2024: Biology
https://read.qxmd.com/read/38534423/research-progress-of-nanomaterials-acting-on-nk-cells-in-tumor-immunotherapy-and-imaging
#17
REVIEW
Yachan Feng, Haojie Zhang, Jiangtao Shao, Chao Du, Xiaolei Zhou, Xueling Guo, Yingze Wang
The prognosis for cancer patients has declined dramatically in recent years due to the challenges in treating malignant tumors. Tumor immunotherapy, which includes immune target inhibition and chimeric antigen receptor cell treatment, is currently evolving quickly. Among them, natural killer (NK) cells are gradually becoming another preferred cell immunotherapy after T cell immunotherapy due to their unique killing effects in innate and adaptive immunity. NK cell therapy has shown encouraging outcomes in clinical studies; however, there are still some problems, including limited efficacy in solid tumors, inadequate NK cell penetration, and expensive treatment expenses...
February 27, 2024: Biology
https://read.qxmd.com/read/38534399/modulating-cholesterol-metabolism-via-acat1-knockdown-enhances-anti-b-cell-lymphoma-activities-of-cd19-specific-chimeric-antigen-receptor-t-cells-by-improving-the-cell-activation-and-proliferation
#18
JOURNAL ARTICLE
Qiong Su, Jie Yao, Muhammad Asad Farooq, Iqra Ajmal, Yixin Duan, Cong He, Xuefei Hu, Wenzheng Jiang
CD19-specific CAR-T immunotherapy has been extensively studied for the treatment of B-cell lymphoma. Recently, cholesterol metabolism has emerged as a modulator of T lymphocyte function and can be exploited in immunotherapy to increase the efficacy of CAR-based systems. Acetyl-CoA acetyltransferase 1 (ACAT1) is the major cholesterol esterification enzyme. ACAT1 inhibitors previously shown to modulate cardiovascular diseases are now being implicated in immunotherapy. In the present study, we achieved knockdown of ACAT1 in T cells via RNA interference technology by inserting ACAT1-shRNA into anti-CD19-CAR-T cells...
March 21, 2024: Cells
https://read.qxmd.com/read/38534352/recombinant-acetylcholine-receptor-immunization-induces-a-robust-model-of-experimental-autoimmune-myasthenia-gravis-in-mice
#19
JOURNAL ARTICLE
Lukas Theissen, Christina B Schroeter, Niklas Huntemann, Saskia Räuber, Vera Dobelmann, Derya Cengiz, Alexander Herrmann, Kathrin Koch-Hölsken, Norbert Gerdes, Hao Hu, Philipp Mourikis, Amin Polzin, Malte Kelm, Hans-Peter Hartung, Sven G Meuth, Christopher Nelke, Tobias Ruck
Myasthenia gravis (MG) is a prototypical autoimmune disease of the neuromuscular junction (NMJ). The study of the underlying pathophysiology has provided novel insights into the interplay of autoantibodies and complement-mediated tissue damage. Experimental autoimmune myasthenia gravis (EAMG) emerged as a valuable animal model, designed to gain further insight and to test novel therapeutic approaches for MG. However, the availability of native acetylcholine receptor (AChR) protein is limited favouring the use of recombinant proteins...
March 14, 2024: Cells
https://read.qxmd.com/read/38534340/mechanical-stress-induces-sodium-entry-and-osmoprotective-responses-in-murine-synovial-fibroblasts
#20
JOURNAL ARTICLE
Annemarie Proff, Ute Nazet, Agnes Schröder, Jonathan Jantsch
Osteoarthritis (OA) is a multifactorial disease depending on molecular, genetic, and environmental factors like mechanical strain. Next to the cartilage and the subchondral bone, OA also affects the synovium, which is critically involved in the maintenance of joint homeostasis. As there is a correlation between the extracellular sodium content in the knee joint and OA, this study investigates the impact of sodium on OA-associated processes like inflammation and bone remodeling without and with mechanical loading in synovial fibroblasts...
March 13, 2024: Cells
keyword
keyword
31855
1
2
Fetch more papers »
Fetching more papers... Fetching...
Remove bar
Read by QxMD icon Read
×

Save your favorite articles in one place with a free QxMD account.

×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"

We want to hear from doctors like you!

Take a second to answer a survey question.