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Hai-Rong Zhu, Ren-Zheng Huang, Xiang-Nan Yu, Xuan Shi, Enkhnaran Bilegsaikhan, Hong-Ying Guo, Guang-Qi Song, Shu-Qiang Weng, Ling Dong, Harry L A Janssen, Xi-Zhong Shen, Ji-Min Zhu
Hepatocellular carcinoma (HCC) remains a major health problem for delayed diagnosis, inefficient surveillance and poor prognosis. Recent studies have indicated that non-coding RNAs contribute to the development of new strategies for diagnosis and treatment of HCC. In the present study, we employed 18 pairs of HCC and matched non-tumor tissues for the identification of differentially expressed microRNAs (miRNAs) in HCC, among which 7 paired specimens were selected randomly for microarray detection. Totally, twenty-three miRNAs were screened out to have statistically significant differences with the threshold of P < 0...
2018: Tohoku Journal of Experimental Medicine
Zheng Li, Chao Jiang, Canhua Ye, Shibai Zhu, Xi Chen, William Kk Wu, Wenwei Qian
Steroid-induced osteonecrosis of the femoral head (ONFH) is a common orthopedic disease. The lack of specific manifestations and effective diagnostic methods make it difficult for this disease to be diagnosed at early stages. Recent studies have shown that microRNAs (miRNA) participate in the development of steroid-induced ONFH, but there is limited research into the diagnostic use of circulating miRNAs. Blood samples from 23 human subjects (7 systemic lupus erythematosus (SLE) patients with steroid-induced ONFH; 7 SLE controls without ONFH; and 9 healthy controls) and 71 rats (19 with steroid-induced ONFH; 28 receiving steroids without ONFH; and 24 untreated controls) were collected to verify the abundance of changes of 6 previously identified ONFH-associated plasma miRNAs (miR-423-5p, miR-99a-5p, miR-10a-5p, miR-21-5p, miR-130a-3p and miR-6787-5p) by quantitative RT-PCR (Reverse Transcription-Polymerase Chain Reaction)...
2018: American Journal of Translational Research
Jun Dong, Zhenhui Zhang, Hongshen Huang, Pei Mo, Chuanfan Cheng, Jianwei Liu, Weizhao Huang, Chaowei Tian, Chongyu Zhang, Jiao Li
BACKGROUND: Aging is one of the key factors that regulate the function of human bone marrow mesenchymal stem cells (hBM-MSCs) and related changes in microRNA (miRNA) expression. However, data reported on aging-related miRNA changes in hBM-MSCs are limited. METHODS: We demonstrated previously that miR-10a is significantly decreased in aged hBM-MSCs and restoration of the miR-10a level attenuated cell senescence and increased the differentiation capacity of aged hBM-MSCs by repressing Krüpple-like factor 4 (KLF4)...
May 30, 2018: Stem Cell Research & Therapy
Gokhan Gumus, Dilek Giray, Onur Bobusoglu, Lulufer Tamer, Derya Karpuz, Olgu Hallioglu
MicroRNAs (miRNAs) are fine regulators of gene expression which participate in the regulation of almost every phase of cell physiology, including development of immune cells and adjustment of immune response. In the studies with in vitro/in vivo model systems, specific miRNAs are revealed to have various roles in cardiovascular development and physiological functions. Furthermore, some studies have been done to understand the role of miRNAs about myocarditis, heart failure and coronary artery diseases. miRNAs crucial role in the pathogenesis of other rheumatic diseases have been investigated, however rheumatic carditis was not studied...
May 29, 2018: Rheumatology International
Yuanyuan Wei, Judit Corbalán-Campos, Rashmi Gurung, Lucia Natarelli, Mengyu Zhu, Nicole Exner, Florian Erhard, Franziska Greulich, Claudia Geißler, N Henriette Uhlenhaut, Ralf Zimmer, Andreas Schober
Background -Alternative macrophage activation, which relies on mitochondrial oxidative metabolism, plays a central role in resolution of inflammation and prevents atherosclerosis. Moreover, macrophages handle large amounts of cholesterol and triglycerides derived from the engulfed modified lipoproteins during atherosclerosis. Although several microRNAs regulate macrophage polarization, the role of microRNA-generating enzyme Dicer in macrophage activation during atherosclerosis is unknown. Methods -To evaluate the role of Dicer in atherosclerosis, Apoe-/- mice with or without macrophage-specific Dicer deletion were fed a high-fat diet for 12 weeks...
May 10, 2018: Circulation
B R Ipson, M B Fletcher, S E Espinoza, A L Fisher
Frailty is a geriatric syndrome associated with progressive physical decline and significantly increases risk for falls, disability, hospitalizations, and death. However, much remains unknown regarding the biological mechanisms that contribute to aging and frailty, and to date, there are no clinically used prognostic or diagnostic molecular biomarkers. The present study profiled exosome-derived microRNAs isolated from the plasma of young, robust older, and frail older individuals and identified eight miRNAs that are uniquely enriched in frailty: miR-10a-3p, miR-92a-3p, miR-185-3p, miR-194-5p, miR-326, miR-532-5p, miR-576-5p, and miR-760...
2018: Journal of Frailty & Aging
Megha Rajasekhar, Ulf Schmitz, Stephane Flamant, Justin J-L Wong, Charles G Bailey, William Ritchie, Jeff Holst, John E J Rasko
Myelopoiesis involves differentiation of hematopoietic stem cells to cellular populations that are restricted in their self-renewal capacity, beginning with the common myeloid progenitor (CMP) and leading to mature cells including monocytes and granulocytes. This complex process is regulated by various extracellular and intracellular signals including microRNAs (miRNAs). We characterised the miRNA profile of human CD34+ CD38+ myeloid progenitor cells, and mature monocytes and granulocytes isolated from cord blood using TaqMan Low Density Arrays...
May 8, 2018: Scientific Reports
Nariman Moradi, Reza Fadaei, Reza Ahmadi, Elham Kazemian, Soudabeh Fallah
BACKGROUND: Coronary artery disease (CAD) is the leading cause of death worldwide. Atherosclerosis, the main underlying cause of CAD, is a progressive inflammatory disease. microRNAs play a substantial role in the inflammatory process and pathogenesis of atherosclerosis. miR-155, a widely studied microRNA, is associated with inflammation but there are conflicting data regarding expression of miR-155 in CAD. miR-10a is also one of the key regulators of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) signaling pathway which have not been evaluated in peripheral blood mononuclear cells (PBMCs) of CAD patients...
May 1, 2018: Clinical Laboratory
Xiaofan Guo, Wei Qiu, Qinglin Liu, Mingyu Qian, Shaobo Wang, Zongpu Zhang, Xiao Gao, Zihang Chen, Hao Xue, Gang Li
While immunosuppressive environments mediated by myeloid-derived suppressor cells (MDSCs) have been well documented in glioma patients, the mechanisms of MDSC development and activation have not been clearly defined. Here, we elucidated a role for glioma-derived exosomes (GDEs) in potentiating an MDSC pathway. We isolated normoxia-stimulated and hypoxia-stimulated GDEs and studied their MDSC induction abilities in vivo and in vitro. Analyses of spleen and bone marrow MDSC proportions (flow cytometry) and reactive oxygen species (ROS), arginase activity, nitric oxide (NO), T-cell proliferation and immunosuppressive cytokine (IL-10 and TGF-β, ELISA) levels were used to assess MDSC expansion and functional capacity...
May 1, 2018: Oncogene
Milena Dürrbaum, Christine Kruse, K Julia Nieken, Bianca Habermann, Zuzana Storchová
BACKGROUND: Aneuploidy, or abnormal chromosome numbers, severely alters cell physiology and is widespread in cancers and other pathologies. Using model cell lines engineered to carry one or more extra chromosomes, it has been demonstrated that aneuploidy per se impairs proliferation, leads to proteotoxic as well as replication stress and triggers conserved transcriptome and proteome changes. RESULTS: In this study, we analysed for the first time miRNAs and demonstrate that their expression is altered in response to chromosome gain...
March 14, 2018: BMC Genomics
Fanyang Kong, Lei Li, Guokun Wang, Xuan Deng, Zhaoshen Li, Xiangyu Kong
No abstract text is available yet for this article.
April 25, 2018: Gut
Honghai Hong, Haihui Yang, Yong Xia
BACKGROUND: Anti-inflammatory drugs and DMARDs, especially methotrexate, have dramatically improved the outlook of RA patient. However, in some patients the treatment effect is still not satisfactory and needs to change therapeutic regimen in time. So, prediction of patient treatment effect timely is particularly important. METHODS: miRNAs are a large family of highly conserved noncoding genes and some of them become potential biomarker of disease diagnosis. In present study, we found circulating miR-10a was significantly decreased in RA patients compared to osteoarthritic patients and healthy people...
April 17, 2018: Current Pharmaceutical Biotechnology
Marwa Tantawy, Mariam G Elzayat, Dina Yehia, Hala Taha
Understanding pediatric brain tumor biology is essential to help on disease stratification, and to find novel markers for early diagnosis. MicroRNA (miRNA) expression has been linked to clinical outcomes and tumor biology. Here, we aimed to detect the expression of different miRNAs in different pediatric brain tumor subtypes to discover biomarkers for early detection and develop novel therapies. Expression of 82 miRNAs was detected in 120 pediatric brain tumors from fixed-formalin paraffin-embedded tissues, low-grade glioma, high-grade glioma, ependymoma, and medulloblastoma, using quantitative real-time PCR...
January 2018: Genetics and Molecular Biology
Guangbing Xiong, Hua Huang, Mengyu Feng, Gang Yang, Suli Zheng, Lei You, Lianfang Zheng, Ya Hu, Taiping Zhang, Yupei Zhao
BACKGROUND: By regulating target genes, microRNAs play essential roles in carcinogenesis and drug resistance in human pancreatic ductal adenocarcinoma (PDAC). Previous studies have shown that microRNA-10a-5p (miR-10a-5p) is overexpressed in PDAC and acts as an oncogene to promote the metastatic behavior of PDAC cells. However, the role of miR-10a-5p in PDAC chemoresistance remains unclear. METHODS: The effects of miR-10a-5p on biological behaviors were analyzed...
April 3, 2018: Journal of Experimental & Clinical Cancer Research: CR
Laixi Bi, Lan Sun, Zhenlin Jin, Shenghui Zhang, Zhijian Shen
MicroRNAs (miRs) have been demonstrated to perform important roles in normal hematopoiesis and leukemogenesis. Accumulating evidence suggests that miR-10a and miR-10b may behave as novel oncogenes or tumor suppressors in human cancer. The present study reported the function of the miR-10 family in myeloid differentiation and acute myeloid leukemia (AML). The levels of miR-10a/b expression were increased in AML cases compared with normal controls, particularly in M1, M2 and M3 subtypes. The levels of miR-10a/b expression were also upregulated in patients with nucleophosmin-mutated AML and AML patients with t(8;21) and t(9;11), compared with the normal control...
April 2018: Oncology Letters
Nazim Hussain, Wenhua Zhu, Congshan Jiang, Jing Xu, Manman Geng, Xiaoying Wu, Safdar Hussain, Bo Wang, Muhammad Shahid Riaz Rajoka, Yue Li, Juan Tian, Liesu Meng, Shemin Lu
Synoviocytes from rheumatoid arthritis (RA) patients share certain features with tumor cells, such as over proliferation and invasion. Anomalous microRNA (miRNA) expression may participate in the pathogenesis of RA in different ways. The objective of the present study was to observe the role of miR-10a-5p targeting T-box transcription factor 5 (TBX5) gene on synoviocyte proliferation and apoptosis in RA. Human synovial sarcoma cell line, SW982 cells stimulating with interleukin-1β (IL-1β) were transfected with miR-10a-5p mimic and siRNA of TBX5...
April 27, 2018: Bioscience Reports
Ding-Yu Lee, Tung-Lin Yang, Yi-Hsuan Huang, Chih-I Lee, Li-Jing Chen, Yu-Tsung Shih, Shu-Yi Wei, Wei-Li Wang, Chih-Cheng Wu, Jeng-Jiann Chiu
BACKGROUND AND AIMS: MicroRNA (miR)-10a is a shear-regulated miR with the lowest expression in vascular endothelial cells (ECs) in athero-susceptible regions with oscillatory shear stress (OS). The aim of this study is to elucidate the relationship between EC miR-10a and atherosclerosis and develop a hemodynamics-based strategy for atherosclerosis treatment. METHODS: A combination of in vitro flow system and in vivo experimental animals was used to examine the functional roles of EC miR-10a and its clinical applications in atherosclerosis...
April 2018: Atherosclerosis
Lin Luo, Ze-Ping Yu, Han Qin, Ze-Xin Zhu, Ming-Heng Liao, Hao-Tian Liao, Ke-Fei Yuan, Yong Zeng
Background: MicroRNAs (miRNAs) have been reported to play vital roles in liver regeneration. Previous studies mainly focused on the functions of intracellular miRNAs, while the functions of circulating exosomal miRNAs in liver regeneration remain largely unknown. The aim of this study was to identify the key exosomal miRNA that played vital roles in liver regeneration. Methods: The Sprague-Dawley male rats were assigned to 70% partially hepatectomized group (n = 6) and sham surgery group (n = 6)...
February 20, 2018: Chinese Medical Journal
Tessho Maruyama, Kazuhide Nishihara, Masato Umikawa, Akira Arasaki, Toshiyuki Nakasone, Fumikazu Nimura, Akira Matayoshi, Kimiko Takei, Saori Nakachi, Ken-Ichi Kariya, Naoki Yoshimi
MicroRNAs (miRs) are expected to serve as prognostic tools for cancer. However, many miRs have been reported as prognostic markers of recurrence or metastasis in oral squamous cell carcinoma patients. We aimed to determine the prognostic markers in early-stage tongue squamous cell carcinoma (TSCC). Based on previous studies, we hypothesized that miR-10a, 10b, 196a-5p, 196a-3p, and 196b were prognostic markers and we retrospectively performed miR expression analyses using formalin-fixed paraffin-embedded sections of surgical specimens...
February 2018: Oncology Letters
Chao Shang, Wei Tang, Chen Pan, Xuanhao Hu, Yang Hong
PURPOSE: Human glioblastoma multiforme (GBM) is the most malignant intracranial primary cancer and is associated with high mortality and poor prognosis. This study aimed to investigate the regulatory effects and mechanism of tumor suppressor candidate 7 (TUSC7) gene to malignant proliferation and chemotherapy resistance to temozolomide (TMZ) in glioma cells. METHODS: The expression of TUSC7 was detected by quantitative real-time PCR. CCK-8 assay was used to detect cell proliferation ability and chemosensitivity...
April 2018: Cancer Chemotherapy and Pharmacology
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