keyword
MENU ▼
Read by QxMD icon Read
search

NAAT

keyword
https://www.readbyqxmd.com/read/28632482/staff-associate-specialist-and-specialty-doctors-national-audit-on-the-management-of-gonorrhoea-in-the-united-kingdom-2015
#1
Helen Mullan, Jane Richards, John Lee
The British Association for Sexual Health and HIV (BASHH) revised United Kingdom national guideline for the management of gonorrhoea in adults, 2011, identified five auditable outcome measures, namely, that all patients should receive first-line treatment, be screened or treated for chlamydial infection, have a test of cure (TOC), be offered written information and have partner notification carried out. The UK National Guideline for Gonorrhoea Testing, Clinical Effectiveness Group, BASHH, 2012, recommended in addition that all reactive nucleic acid amplification tests (NAATs) from pharynx and rectum should be confirmed by supplementary testing, using a second NAAT which detects a different nucleic acid target, all those with a positive NAAT for gonorrhoea should have culture and antimicrobial susceptibility testing and that TOC should be done by two weeks...
January 1, 2017: International Journal of STD & AIDS
https://www.readbyqxmd.com/read/28632278/paper-microfluidics-for-nucleic-acid-amplification-testing-naat-of-infectious-diseases
#2
Laura Magro, Camille Escadafal, Pierre Garneret, Béatrice Jacquelin, Aurélia Kwasiborski, Jean-Claude Manuguerra, Fabrice Monti, Anavaj Sakuntabhai, Jessica Vanhomwegen, Pierre Lafaye, Patrick Tabeling
The diagnosis of infectious diseases is entering a new and interesting phase. Technologies based on paper microfluidics, coupled to developments in isothermal amplification of Nucleic Acids (NAs) raise opportunities for bringing the methods of molecular biology in the field, in a low setting environment. A lot of work has been performed in the domain over the last few years and the landscape of contributions is rich and diverse. Most often, the level of sample preparation differs, along with the sample nature, the amplification and detection methods, and the design of the device, among other features...
June 20, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28612378/trichomonas-vaginalis-is-very-rare-among-women-with-vaginal-discharge-in-podlaskie-province-poland
#3
Agnieszka Beata Serwin, Violetta Bulhak-Koziol, Marianna Sokolowska, Daniel Golparian, Magnus Unemo
Trichomonas vaginalis is the most common curable sexually transmitted pathogen globally. However, in the European Union (EU), trichomoniasis appears to be a rare condition. The aim of this study was to examine the prevalence of T. vaginalis among females attending an STI centre in Bialystok, Poland, using the highly sensitive and specific APTIMA T. vaginalis assay. Consecutive females, referred by gynaecologists mainly because of abnormal vaginal discharge, were diagnosed using wet mount microscopy, culture and APTIMA T...
June 14, 2017: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/28583076/diagnostic-accuracy-of-nucleic-acid-amplification-tests-naats-in-urine-for-genitourinary-tuberculosis-a-systematic-review-and-meta-analysis
#4
Carlos Altez-Fernandez, Victor Ortiz, Majid Mirzazadeh, Luis Zegarra, Carlos Seas, Cesar Ugarte-Gil
BACKGROUND: Genitourinary tuberculosis is the third most common form of extrapulmonary tuberculosis. Diagnosis is difficult because of unspecific clinical manifestations and low accuracy of conventional tests. Unfortunately, the delayed diagnosis impacts the urinary tract severely. Nucleic acid amplification tests yield fast results, and among these, new technologies can also detect drug resistance. There is lack of consensus regarding the use of these tests in genitourinary tuberculosis; we therefore aimed to assess the accuracy of nucleic acid amplification tests in the diagnosis of genitourinary tuberculosis and to evaluate the heterogeneity between studies...
June 5, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28562672/rapid-detection-and-strain-typing-of-chlamydia-trachomatis-using-a-highly-multiplexed-microfluidic-pcr-assay
#5
Rosemary S Turingan, Ludmila Kaplun, Greice Krautz-Peterson, Sarah Norsworthy, Anna Zolotova, Sandeep J Joseph, Timothy D Read, Deborah Dean, Eugene Tan, Richard F Selden
Nucleic acid amplification tests (NAATs) are recommended by the CDC for detection of Chlamydia trachomatis (Ct) urogenital infections. Current commercial NAATs require technical expertise and sophisticated laboratory infrastructure, are time-consuming and expensive, and do not differentiate the lymphogranuloma venereum (LGV) strains that require a longer duration of treatment than non-LGV strains. The multiplexed microfluidic PCR-based assay presented in this work simultaneously interrogates 13 loci to detect Ct and identify LGV and non-LGV strain-types...
2017: PloS One
https://www.readbyqxmd.com/read/28555402/clostridium-difficile-infections-in-finland-2008-2015-trends-diagnostics-and-ribotypes
#6
S Mentula, S M Kotila, O Lyytikäinen, S Ibrahem, J Ollgren, A Virolainen
We evaluated Clostridium difficile (CD) diagnostics in Finnish clinical microbiology laboratories during 2006-2011, with an update in 2015, in relation to CD surveillance data of the National Infectious Disease Register (NIDR) and ribotyping data from the national reference laboratory during the years 2008-2015. In 2011, diagnostic activity varied regionally more than three-fold and the positivity rate ranged between 7 and 21%. Nucleic acid amplification testing (NAAT) was implemented in the regions with high activity and NAAT users tested 30% more patients and found 15% more cases per population than those not using it...
May 30, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28549882/trends-in-the-use-of-laboratory-tests-for-the-diagnosis-of-clostridium-difficile-infection-and-association-with-incidence-rates-in-quebec-canada-2010-2014
#7
C Bogaty, S Lévesque, C Garenc, C Frenette, D Bolduc, L-A Galarneau, C Lalancette, V Loo, C Tremblay, M Trudeau, J Vachon, M Dionne, J Villeneuve, J Longtin, Y Longtin
BACKGROUND: Several Clostridium difficile infection (CDI) surveillance programs do not specify laboratory strategies to use. We investigated the evolution in testing strategies used across Quebec, Canada, and its association with incidence rates. METHODS: Cross-sectional study of 95 hospitals by surveys conducted in 2010 and in 2013-2014. The association between testing strategies and institutional CDI incidence rates was analyzed via multivariate Poisson regressions...
May 23, 2017: American Journal of Infection Control
https://www.readbyqxmd.com/read/28544969/prior-treatment-with-non-anti-tb-antibiotics-and-the-duration-of-symptoms-have-no-effect-on-diagnostics-of-tuberculous-meningitis
#8
Marcin Paciorek, Andrzej Pihowicz, Dominik Bursa, Joanna Schöntaler Humięcka, Justyna D Kowalska, Mateusz Antosiewicz, Magda E Thompson, Agata Skrzat-Wojdacz, Agnieszka Bednarska, Andrzej Horban
PURPOSE: Our objective was to investigate whether diagnosis of tuberculous meningitis (TBM) with microbiological and molecular analysis was affected by prior empirical non anti tuberculosis antibiotics or by duration of symptoms before lumbar puncture. MATERIALS AND METHODS: We retrospectively evaluated medical records of patients with TBM confirmed by positive culture, nucleic acid amplification techniques (NAATs) or Ehrlich-Ziehl-Neelsen staining (EZNs) from the cerebrospinal fluid (CSF) or by characteristic results of biochemical analysis of CSF combined with a typical clinical manifestation...
May 22, 2017: Advances in Medical Sciences
https://www.readbyqxmd.com/read/28540697/in-silico-pcr-tools-for-a-fast-primer-probe-and-advanced-searching
#9
Ruslan Kalendar, Alexandr Muterko, Malika Shamekova, Kabyl Zhambakin
The polymerase chain reaction (PCR) is fundamental to molecular biology and is the most important practical molecular technique for the research laboratory. The principle of this technique has been further used and applied in plenty of other simple or complex nucleic acid amplification technologies (NAAT). In parallel to laboratory "wet bench" experiments for nucleic acid amplification technologies, in silico or virtual (bioinformatics) approaches have been developed, among which in silico PCR analysis. In silico NAAT analysis is a useful and efficient complementary method to ensure the specificity of primers or probes for an extensive range of PCR applications from homology gene discovery, molecular diagnosis, DNA fingerprinting, and repeat searching...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28490486/meatal-swabs-contain-less-cellular-material-and-are-associated-with-a-decrease-in-gram-stain-smear-quality-compared-to-urethral-swabs-in-men
#10
Stephen J Jordan, Jane R Schwebke, Kristal J Aaron, Barbara Van Der Pol, Edward W Hook
Urethral swabs are the test of choice for point-of-care Gram stain testing to diagnose Neisseria gonorrhoeae (NG) and non-gonococcal urethritis (NGU) in men. As an alternative to urethral swabs, meatal swabs have been recommended for collection of urethral discharge to diagnose NG and Chlamydia trachomatis (CT) in certain populations by nucleic acid amplification testing (NAAT), as they are a less invasive collection method. However, as meatal swabs could be sampling a reduced surface area and result in fewer collected epithelial cells when compared to urethral swabs, the adequacy of meatal swab specimens to collect sufficient cellular material for Gram stain testing remains unknown...
May 10, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28475636/characterisation-of-the-nicotianamine-aminotransferase-and-deoxymugineic-acid-synthase-genes-essential-to-strategy-ii-iron-uptake-in-bread-wheat-triticum-aestivum-l
#11
Jesse T Beasley, Julien P Bonneau, Alexander A T Johnson
Iron (Fe) uptake in graminaceous plant species occurs via the release and uptake of Fe-chelating compounds known as mugineic acid family phytosiderophores (MAs). In the MAs biosynthetic pathway, nicotianamine aminotransferase (NAAT) and deoxymugineic acid synthase (DMAS) enzymes catalyse the formation of 2'-deoxymugineic acid (DMA) from nicotianamine (NA). Here we describe the identification and characterisation of six TaNAAT and three TaDMAS1 genes in bread wheat (Triticum aestivum L.). The coding sequences of all six TaNAAT homeologs consist of seven exons with ≥88...
2017: PloS One
https://www.readbyqxmd.com/read/28465576/paper-based-rna-detection-and-multiplexed-analysis-for-ebola-virus-diagnostics
#12
Laura Magro, Béatrice Jacquelin, Camille Escadafal, Pierre Garneret, Aurélia Kwasiborski, Jean-Claude Manuguerra, Fabrice Monti, Anavaj Sakuntabhai, Jessica Vanhomwegen, Pierre Lafaye, Patrick Tabeling
The most performing techniques enabling early diagnosis of infectious diseases rely on nucleic acid detection. Today, because of their high technicality and cost, nucleic acid amplification tests (NAAT) are of benefit only to a small fraction of developing countries population. By reducing costs, simplifying procedures and enabling multiplexing, paper microfluidics has the potential to considerably facilitate their accessibility. However, most of the studies performed in this area have not quit the lab. This letter brings NAAT on paper closer to the field, by using clinical samples and operating in a resource-limited setting...
May 2, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28409584/atypical-syphilitic-chancre-of-the-tongue-in-a-hiv-positive-patient-the-diagnostic-role-of-naats
#13
Stefano Ramoni, Vinicio Boneschi, Marco Cusini
No abstract text is available yet for this article.
June 2017: Giornale Italiano di Dermatologia e Venereologia: Organo Ufficiale, Società Italiana di Dermatologia e Sifilografia
https://www.readbyqxmd.com/read/28397965/comparison-of-three-multiplex-pcr-assays-for-detection-of-respiratory-viruses-anyplex-ii-rv16-advansure-rv-and-real-q-rv
#14
Seung Gyu Yun, Min Young Kim, Jong Moon Choi, Chang Kyu Lee, Chae Seung Lim, Yunjung Cho, In Bum Suh
BACKGROUND: Due to its great sensitivity, the nucleic acid amplification test (NAAT) is widely used for detection of respiratory viruses (RV). However, few reports have described a direct comparison between multiplex RT-PCR assays for RV. The objective of this study was to perform a direct comparison of three multiplex RT-PCR assays for the detection of respiratory viruses. METHODS: A total of 201 respiratory samples (161 nasopharyngeal swab samples and 40 sputum samples) were tested with three commercial RV assays: Seegene Anyplex II RV16 (AP), LG AdvanSure RV (AD), and Biosewoom Real-Q RV (RQ)...
April 11, 2017: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/28369309/analysis-of-false-negative-human-immunodeficiency-virus-rapid-tests-performed-on-oral-fluid-in-3-international-clinical-research-studies
#15
Marcel E Curlin, Roman Gvetadze, Wanna Leelawiwat, Michael Martin, Charles Rose, Richard W Niska, Tebogo M Segolodi, Kachit Choopanya, Jaray Tongtoyai, Timothy H Holtz, Taraz Samandari, Janet M McNicholl
Background.: The OraQuick Advance Rapid HIV-1/2 Test is a point-of-care test capable of detecting human immunodeficiency virus (HIV)-specific antibodies in blood and oral fluid. To understand test performance and factors contributing to false-negative results in longitudinal studies, we examined results of participants enrolled in the Botswana TDF/FTC Oral HIV Prophylaxis Trial, the Bangkok Tenofovir Study, and the Bangkok MSM Cohort Study, 3 separate clinical studies of high-risk, HIV-negative persons conducted in Botswana and Thailand...
June 15, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/28364412/the-validation-and-evaluation-of-anti-hiv-testing-algorithm-used-in-mobile-clinic-setting-for-men-who-have-sex-with-men-in-metropolitan-bangkok-thailand
#16
Tippawan Pankam, Sarun Saensiriphan, Suphackok Areeyolwattana, Napapat Barisri, Supabhorn Pengnonyang, Sunee Sirivichayakul, Nittaya Phanuphak, Praphan Phanuphak
BACKGROUND: Same-day anti-HIV testing algorithm is recommended by Thai National Guidelines. We compared performance characteristics of algorithms used in a mobile clinic and a facility-based clinic for men who have sex with men (MSM) in Bangkok. METHODS: Mobile clinic samples collected from 4 saunas and 2 spa venues were tested by Alere DetermineTM HIV 1/2, followed by DoubleCheck GoldTM Ultra HIV 1/2 and SD Bioline HIV 1/2 3.0. All samples were re-tested at the Thai Red Cross Anonymous Clinic (TRCAC) by Architect HIV Ag/Ab or Elecsys HIV combi PT, followed by Alere DetermineTM HIV 1/2 and Serodia HIV 1/2...
April 1, 2017: Asian Pacific Journal of Allergy and Immunology
https://www.readbyqxmd.com/read/28351689/detection-of-new-hiv-infections-in-a-multicentre-hiv-antiretroviral-pre-exposure-prophylaxis-trial
#17
Katrien Fransen, Irith de Baetselier, Elizabeth Rammutla, Khatija Ahmed, Frederick Owino, Walter Agingu, Gustav Venter, Jen Deese, Lut Van Damme, Tania Crucitti
BACKGROUND: Monthly specimens collected from FEM-PrEP-a Phase III trial [1] were investigated for the detection of acute HIV (AHI) infection. OBJECTIVES: To evaluate the efficiency of the study-specific HIV algorithm in detecting AHI, and the performance of each of the serological and molecular tests used in diagnosing new infections, and their contribution to narrowing the window period. STUDY DESIGN: A total of 83 pre-seroconversion specimens from 61 seroconverters from the FEM-PrEP trial were further analyzed in a sub-study...
March 20, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28317800/are-who-approved-nucleic-acid-amplification-tests-causing-large-scale-false-identification-of-rifampicin-resistant-tuberculosis-programmatic-experience-from-south-india
#18
Praveen Sanker, Anusree P Ambika, Vishnu T Santhosh, Ramya Puthukkudi Kottuthodi, Ravikrishnan Balakrishnan, Sunil Kumar Mrithunjayan, Hisham Moosan
INTRODUCTION: The nucleic acid amplification tests (NAATs): Line probe assay and GeneXpert (Xpert) have evolved as the primary tool for identification of rifampicin (RIF)-resistant (RR) tuberculosis (TB) worldwide, primarily because of the ease and speed. We rechecked RR isolates identified by NAATs from presumptive RR TB cases belonging to South India by the Revised National TB Control Program, India using multiple RIF concentrations on Bactec MGIT system and compared the mutation patterns with the resistance levels...
January 2017: International Journal of Mycobacteriology
https://www.readbyqxmd.com/read/28290950/survey-of-sexually-transmitted-disease-laboratory-methods-in-u-s-army-laboratories-2012
#19
Nicole K Leamer, Nikki N Jordan, Laura A Pacha, Nabil H Latif, Eric C Garges, Joel C Gaydos
INTRODUCTION: Sexually transmitted diseases (STD) affect primarily young people (17-24 years). The U.S. Military, with many young people, strives to maintain effective STD treatment and prevention programs using current methods. Laboratory testing technology and capacity are important for appropriate clinical management and to provide data to direct prevention programs. STD laboratory capabilities are assessed in civilian and military laboratories using surveys. An Army laboratory survey was conducted in 2007...
March 2017: Military Medicine
https://www.readbyqxmd.com/read/28283408/development-and-evaluation-of-an-enzyme-linked-immunosorbent-assay-for-the-detection-of-antibodies-to-a-common-urogenital-derivative-of-chlamydia-trachomatis-plasmid-encoded-pgp3
#20
Catherine E Winstanley, Kyle H Ramsey, Peter Marsh, Ian N Clarke
BACKGROUND: Urogenital infection with Chlamydia trachomatis is the most commonly diagnosed sexually transmitted infection in the developed world. Accurate measurement and therefore understanding the seroprevalence of urogenital C. trachomatis infections requires a rigorously optimised and validated ELISA. Previous ELISAs based on the C. trachomatis plasmid-encoded protein, PGP3, have been described but lack standardisation and critical controls or use a less common PGP3 as the capture antigen...
March 7, 2017: Journal of Immunological Methods
keyword
keyword
3185
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"