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https://www.readbyqxmd.com/read/27927919/clinical-evaluation-and-cost-analysis-of-the-great-basin-shiga-toxin-direct-molecular-assay-for-the-detection-of-shiga-toxin-producing-escherichia-coli-stec-in-diarrheal-stool-specimens
#1
Matthew L Faron, Nathan A Ledeboer, Jessica Connolly, Paul A Granato, Brenda R Alkins, Jennifer Dien Bard, Judy A Daly, Stephen Young, Blake W Buchan
The Shiga Toxin Direct molecular assay (ST Direct) relies on nucleic acid amplification and solid array-based amplicon detection to identify Shiga toxin-encoding E. coli (STEC) in preserved stool specimens. Genes encoding Shiga toxin (stx1, stx2), as well as the E. coli serotype O:157-specific marker rfbE, are simultaneously detected within 2 hours. ST Direct was evaluated using 1,084 prospectively collected preserved stool specimens across five clinical centers. An additional 55 retrospectively collected, frozen specimens were included to increase the number of positive specimens evaluated...
December 7, 2016: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/27876339/diagnosis-and-treatment-of-clostridium-difficile-c-diff-colitis-review-of-the-literature-and-a-perspective-in-gynecologic-oncology
#2
REVIEW
Kristin N Taylor, Michael T McHale, Cheryl C Saenz, Steven C Plaxe
Clostridium difficile infection (CDI) is a major cause of nosocomial diarrhea with the potential for significant morbidity and mortality. Colonization in a susceptible individual, with risk factors such as prior antibiotic use, advanced age, or medical comorbidities, may result in symptomatic infection. Although patients with a gynecologic malignancy may be at a higher risk of developing CDI due to an increased likelihood of having one or more risk factors, data do not consistently support the idea that chemotherapy or cancer itself are independently associated with CDI...
November 19, 2016: Gynecologic Oncology
https://www.readbyqxmd.com/read/27864473/a-recommendation-for-timing-of-repeat-chlamydia-trachomatis-test-following-infection-and-treatment-in-pregnant-and-nonpregnant-women
#3
Gweneth B Lazenby, Jeffrey E Korte, Sarah Tillman, Florence K Brown, David E Soper
The objective of this study was to describe the time required to obtain a negative chlamydia test in pregnant and nonpregnant women following treatment to inform test-of-cure collection and recommend an abstinence period to avoid reinfection. Seventy-two women with Chlamydia trachomatis infection, 36 pregnant and 36 nonpregnant, were enrolled in a prospective cohort study. Women were excluded less than 18 years of age, if they had been treated for chlamydia, reported an allergy to macrolide antibiotics, or if they had Myasthenia Gravis...
November 18, 2016: International Journal of STD & AIDS
https://www.readbyqxmd.com/read/27862349/lymphogranuloma-venereum-with-only-proximal-rectal-involvement-mimicking-inflammatory-bowel-disease-a-potential-diagnostic-pitfall
#4
F Rob, J Kašpírková, K Jůzlová, M Pešta, J Hercogová
Lymphogranuloma venereum (LGV) is a sexually transmitted infection (STI) caused by the invasive serovars L1, L2 and L3 of Chlamydia trachomatis (C. trachomatis). [1] The incidence of LGV is rising in many Western countries. [2] The most common symptoms of LGV are proctitis and inguinal lymphadenopathy. LGV mimicking inflammatory bowel disease (IBD) is not a rare condition and has already been described in several reports. [3,4] LGV can affect even more proximal parts of the bowel. [5] Diagnosis of LGV is usually done by positive NAAT for C...
November 15, 2016: Journal of the European Academy of Dermatology and Venereology: JEADV
https://www.readbyqxmd.com/read/27835626/genotype-specific-concordance-of-chlamydia-trachomatis-genital-infection-within-heterosexual-partnerships
#5
Julia A Schillinger, Barry P Katz, Lauri E Markowitz, Phillip G Braslins, Lydia A Shrier, Guillermo Madico, Barbara Van Der Pol, Donald P Orr, Peter A Rice, Byron E Batteiger
BACKGROUND: Sexual transmission rates of Chlamydia trachomatis (Ct) cannot be measured directly; however, the study of concordance of Ct infection in sexual partnerships (dyads) can help to illuminate factors influencing Ct transmission. METHODS: Heterosexual men and women with Ct infection and their sex partners were enrolled and partner-specific coital and behavioral data collected for the prior 30 days. Microbiological data included Ct culture, and nucleic acid amplification testing (NAAT), quantitative Ct polymerase chain reaction, and ompA genotyping...
December 2016: Sexually Transmitted Diseases
https://www.readbyqxmd.com/read/27812208/viability-pcr-shows-that-naat-detects-a-high-proportion-of-dna-from-non-viable-chlamydia-trachomatis
#6
Kevin J H Janssen, Christian J P A Hoebe, Nicole H T M Dukers-Muijrers, Lisanne Eppings, Mayk Lucchesi, Petra F G Wolffs
OBJECTIVES: According to the current guidelines for laboratory diagnosis of sexually transmitted infections (STIs), nucleic acid amplification tests (NAATs) are the preferred diagnostic method for Chlamydia trachomatis (CT) infections. However, NAATs amplify the available target DNA without discriminating between DNA originating from viable or non-viable CT. Assessing CT viability will provide more insights in the clinical and public health relevance of a CT positive test result. The aim of this study was to technically validate and implement viability-PCR (V-PCR) to asses CT viability...
2016: PloS One
https://www.readbyqxmd.com/read/27809795/leucocyte-esterase-dip-stick-test-as-a-point-of-care-diagnostic-for-urogenital-chlamydia-in-male-patients-a-multi-center-evaluation-in-two-sti-outpatient-clinics-in-paramaribo-and-amsterdam
#7
Menne Bartelsman, Henry J C de Vries, Maarten F Schim van der Loeff, Leslie O A Sabajo, Jannie J van der Helm
BACKGROUND: Point-of-care (POC) tests are an important strategy to address the epidemic of sexually transmitted infections (STIs). The leucocyte esterase test (LET) can be used as a POC test for chlamydia. The aim of this study was to determine the diagnostic accuracy of the LET to detect urogenital chlamydia among men at STI clinics in Paramaribo, Suriname and Amsterdam, the Netherlands. METHODS: Recruitment of patients took place in 2008-2010 in Suriname and in 2009-2010 in the Netherlands...
November 3, 2016: BMC Infectious Diseases
https://www.readbyqxmd.com/read/27764165/detection-of-acute-and-early-hiv-1-infections-in-an-hiv-hyper-endemic-area-with-limited-resources
#8
Simnikiwe H Mayaphi, Desmond J Martin, Thomas C Quinn, Oliver Laeyendecker, Steve A S Olorunju, Gregory R Tintinger, Anton C Stoltz
BACKGROUND: Two thirds of the world's new HIV infections are in sub-Saharan Africa. Acute HIV infection (AHI) is the time of virus acquisition until the appearance of HIV antibodies. Early HIV infection, which includes AHI, is the interval between virus acquisition and establishment of viral load set-point. This study aimed to detect acute and early HIV infections in a hyper-endemic setting. METHODS: This was a cross-sectional diagnostic study that enrolled individuals who had negative rapid HIV results in five clinics in South Africa...
2016: PloS One
https://www.readbyqxmd.com/read/27745553/failure-of-risk-adjustment-by-test-method-for-c-difficile-laboratory-identified-event-reporting
#9
Alexandre R Marra, Michael B Edmond, Bradley A Ford, Loreen A Herwaldt, Abdullah R Algwizani, Daniel J Diekema
Using an algorithm including both enzyme immunoassay (EIA) and nucleic acid amplification (NAAT) for Clostridium difficile infection (CDI) diagnosis, we found that the use of NAAT versus EIA almost doubled our hospital-onset CDI laboratory-identified (LabID) event standardized infection ratio (SIR). We recommend that the current risk adjustment approach be modified. Infect Control Hosp Epidemiol 2016:1-3.
October 17, 2016: Infection Control and Hospital Epidemiology
https://www.readbyqxmd.com/read/27724878/time-to-clearance-of-chlamydia-trachomatis-rna-and-dna-after-treatment-in-patients-coinfected-with-neisseria-gonorrhoeae-a-prospective-cohort-study
#10
Carolien M Wind, Maarten F Schim van der Loeff, Magnus Unemo, Rob Schuurman, Alje P van Dam, Henry J C de Vries
BACKGROUND: Performing a test of cure (TOC) could demonstrate success or failure of antimicrobial treatment of Chlamydia trachomatis infection, but recommendations for the timing of a TOC using nucleic acid amplification tests (NAATs) are inconsistent. We assessed time to clearance of C. trachomatis after treatment, using modern RNA- and DNA-based NAATs. METHODS: We analysed data from patients with a C. trachomatis and Neisseria gonorrhoeae coinfection who visited the STI Clinic Amsterdam, The Netherlands, from March through October 2014...
October 11, 2016: BMC Infectious Diseases
https://www.readbyqxmd.com/read/27681919/diagnostic-procedures-to-detect-chlamydia-trachomatis-infections
#11
REVIEW
Thomas Meyer
The intracellular life style of chlamydia and the ability to cause persistent infections with low-grade replication requires tests with high analytical sensitivity to directly detect C. trachomatis (CT) in medical samples. Nucleic acid amplification tests (NAATs) are the most sensitive assays with a specificity similar to cell culture and are considered the method of choice for CT detection. In addition, NAATs can be performed on various clinical specimens that do not depend on specific transport and storage conditions, since NAATs do not require infectious bacteria...
August 5, 2016: Microorganisms
https://www.readbyqxmd.com/read/27638011/antibody-to-chlamydia-trachomatis-proteins-troa-and-htra-as-a-biomarker-for-chlamydia-trachomatis-infection
#12
K Hokynar, S Korhonen, P Norja, J Paavonen, M Puolakkainen
: We studied whether antibody to two chlamydial proteins (TroA and HtrA) could be used as biomarkers of Chlamydia trachomatis infection. METHODS: Recombinant proteins C. trachomatis TroA and HtrA were used as antigens in enzyme immunoassay (EIA). Both IgG and IgA antibody responses were studied. RESULTS: IgG or IgA antibody to either protein was infrequently detected in sera from healthy blood donors or virgin girls. Patients attending the STI Clinic and patients with perihepatitis had often IgG antibody against TroA (25 and 50 % respectively) and HtrA (21 and 38 % respectively)...
September 14, 2016: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/27596232/pharmacokinetics-of-oseltamivir-in-infants-under-the-age-of-1-year
#13
Rashmi Dixit, Slade Matthews, Gulam Khandaker, Karen Walker, Marino Festa, Robert Booy
BACKGROUND: Oseltamivir is the only antiviral treatment recommended for influenza in young children over the age of 1 year. There is scant data on oseltamivir pharmacokinetics (PK) in infants <1 year. We set out to perform PK measurements in infants who received oseltamivir. METHODS: This study was a prospective, uncontrolled, open label evaluation of the pharmacokinetics of oseltamivir metabolism, safety of oseltamivir, viral clearance in infants <12 months diagnosed with influenza by nasopharyngeal influenza nucleic acid antigen test (NAAT)...
December 2016: Clinical and Translational Medicine
https://www.readbyqxmd.com/read/27558177/a-pooled-nucleic-acid-amplification-test-for-screening-of-stool-specimens-for-shiga-toxin-producing-escherichia-coli
#14
Agatha N Jassem, Frank Chou, Cathevine Yang, Matthew A Croxen, Katarina D M Pintar, Ana Paccagnella, Linda Hoang, Natalie Prystajecky
Shiga toxin-producing Escherichia coli (STEC) associated enteric illness is attributed to O157 and non-O157 serotypes, however, traditional culture-based methods under-detect non-O157 STEC. Labor and consumable costs are major barriers for implementation of CDC recommendation of testing all stools for both O157 and non-O157. We evaluated the feasibility of a pooled nucleic acid amplification test (NAAT) as an approach for screening of stool specimens for STEC. For retrospective evaluation, 300 stool specimens were used to create pools of 10...
August 24, 2016: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/27558163/urethral-inflammatory-response-to-ureaplasma-is-significantly-lower-than-to-mycoplasma-genitalium-and-chlamydia-trachomatis
#15
Harald Moi, Nils Reinton, Ivana Randjelovic, Elina J Reponen, Line Syvertsen, Amir Moghaddam
A non-syndromic approach to treatment of people with non-gonococcal urethritis (NGU) requires identification of pathogens and understanding of the role of those pathogens in causing disease. The most commonly detected and isolated micro-organisms in the male urethral tract are bacteria belonging to the family of Mycoplasmataceae, in particular Ureaplasma urealyticum and Ureaplasma parvum To better understand the role of these Ureaplasma species in NGU, we have performed a prospective analysis of male patients voluntarily attending a drop in STI clinic in Oslo...
August 24, 2016: International Journal of STD & AIDS
https://www.readbyqxmd.com/read/27549897/a-rapid-instrument-free-sample-to-result-nucleic-acid-amplification-test
#16
Lisa K Lafleur, Joshua D Bishop, Erin K Heiniger, Ryan P Gallagher, Maxwell D Wheeler, Peter Kauffman, Xiaohong Zhang, Enos C Kline, Joshua R Buser, Sujatha Kumar, Samantha A Byrnes, Nicolaas M J Vermeulen, Noah K Scarr, Yevgeniy Belousov, Walt Mahoney, Bhushan J Toley, Paula D Ladd, Barry R Lutz, Paul Yager
The prototype demonstrated here is the first fully integrated sample-to-result diagnostic platform for performing nucleic acid amplification tests that requires no permanent instrument or manual sample processing. The multiplexable autonomous disposable nucleic acid amplification test (MAD NAAT) is based on two-dimensional paper networks, which enable sensitive chemical detection normally reserved for laboratories to be carried out anywhere by untrained users. All reagents are stored dry in the disposable test device and are rehydrated by stored buffer...
October 7, 2016: Lab on a Chip
https://www.readbyqxmd.com/read/27505296/2016-european-guideline-on-mycoplasma-genitalium-infections
#17
J S Jensen, M Cusini, M Gomberg, H Moi
Mycoplasma genitalium infection contributes to 10-35% of non-chlamydial non-gonococcal urethritis in men. In women, M. genitalium is associated with cervicitis and pelvic inflammatory disease (PID). Transmission of M. genitalium occurs through direct mucosal contact. Asymptomatic infections are frequent. In women, symptoms include vaginal discharge, dysuria or symptoms of PID - abdominal pain and dyspareunia. In men, urethritis, dysuria and discharge predominates. Besides symptoms, indication for laboratory test is a high-risk sexual behaviour...
August 9, 2016: Journal of the European Academy of Dermatology and Venereology: JEADV
https://www.readbyqxmd.com/read/27505006/diagnosis-and-outcome-of-clostridium-difficile-infection-by-toxin-enzyme-immunoassay-and-pcr-in-an-island-population
#18
Shankar Kumar, Richard Pollok, Ivan Muscat, Timothy Planche
BACKGROUNDS AND AIMS: Clostridium difficile infection (CDI) is a potentially life-threatening cause of diarrhoea. Correct laboratory diagnosis is essential to differentiate CDI from other causes of diarrhoea. A positive faecal C.difficile toxin (CDT) is the best indicator of CDI, but the significance of a positive faecal nucleic acid amplification test (NAAT) remains unclear. Our aim was to elucidate the significance of CDI diagnostics in patients in Jersey. METHODS: A retrospective, 5-year study was conducted at an island district general hospital of patients who developed CDI...
August 9, 2016: Journal of Gastroenterology and Hepatology
https://www.readbyqxmd.com/read/27502928/design-of-the-femcure-study-prospective-multicentre-study-on-the-transmission-of-genital-and-extra-genital-chlamydia-trachomatis-infections-in-women-receiving-routine-care
#19
Nicole H T M Dukers-Muijrers, Petra F G Wolffs, Lisanne Eppings, Hannelore M Götz, Sylvia M Bruisten, Maarten F Schim van der Loeff, Kevin Janssen, Mayk Lucchesi, Titia Heijman, Birgit H van Benthem, Jan E van Bergen, Servaas A Morre, Jos Herbergs, Gerjo Kok, Mieke Steenbakkers, Arjan A Hogewoning, Henry J de Vries, Christian J P A Hoebe
BACKGROUND: In women, anorectal infections with Chlamydia trachomatis (CT) are about as common as genital CT, yet the anorectal site remains largely untested in routine care. Anorectal CT frequently co-occurs with genital CT and may thus often be treated co-incidentally. Nevertheless, post-treatment detection of CT at both anatomic sites has been demonstrated. It is unknown whether anorectal CT may play a role in post-treatment transmission. This study, called FemCure, in women who receive routine treatment (either azithromycin or doxycycline) aims to understand the post-treatment transmission of anorectal CT infections, i...
August 8, 2016: BMC Infectious Diseases
https://www.readbyqxmd.com/read/27497595/performance-of-self-collected-penile-meatal-swabs-compared-to-clinician-collected-urethral-swabs-for-the-detection-of-chlamydia-trachomatis-neisseria-gonorrhoeae-trichomonas-vaginalis-and-mycoplasma-genitalium-by-nucleic-acid-amplification-assays
#20
Laura Dize, Perry Barnes, Mathilda Barnes, Yu-Hsiang Hsieh, Vincent Marsiglia, Della Duncan, Justin Hardick, Charlotte A Gaydos
Men were enrolled in a study to assess the performance and acceptability of self-collected penile meatal swabs as compared to clinician-collected urethral swabs for sexually transmitted infections (STIs). We expected penile-meatal swabs to perform favorably to urethral swabs for Chlamydia trachomatis (CT), Neisseria gonorrhoeae (NG), Trichomonas vaginalis (TV), and Mycoplasma genitalium (MG) detection by nucleic acid amplification assays (NAATs). Of 203 swab pairs tested; for CT, penile-meatal swab sensitivity was 96...
October 2016: Diagnostic Microbiology and Infectious Disease
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