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https://www.readbyqxmd.com/read/28409584/atypical-syphilitic-chancre-of-the-tongue-in-a-hiv-positive-patient-the-diagnostic-role-of-naats
#1
Stefano Ramoni, Vinicio Boneschi, Marco Cusini
No abstract text is available yet for this article.
June 2017: Giornale Italiano di Dermatologia e Venereologia: Organo Ufficiale, Società Italiana di Dermatologia e Sifilografia
https://www.readbyqxmd.com/read/28397965/comparison-of-three-multiplex-pcr-assays-for-detection-of-respiratory-viruses-anyplex-ii-rv16-advansure-rv-and-real-q-rv
#2
Seung Gyu Yun, Min Young Kim, Jong Moon Choi, Chang Kyu Lee, Chae Seung Lim, Yunjung Cho, In Bum Suh
BACKGROUND: Due to its great sensitivity, the nucleic acid amplification test (NAAT) is widely used for detection of respiratory viruses (RV). However, few reports have described a direct comparison between multiplex RT-PCR assays for RV. The objective of this study was to perform a direct comparison of three multiplex RT-PCR assays for the detection of respiratory viruses. METHODS: A total of 201 respiratory samples (161 nasopharyngeal swab samples and 40 sputum samples) were tested with three commercial RV assays: Seegene Anyplex II RV16 (AP), LG AdvanSure RV (AD), and Biosewoom Real-Q RV (RQ)...
April 11, 2017: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/28369309/analysis-of-false-negative-hiv-rapid-tests-performed-on-oral-fluid-in-three-international-clinical-research-studies
#3
Marcel E Curlin, Roman Gvetadze, Wanna Leelawiwat, Michael Martin, Charles Rose, Richard W Niska, Tebogo M Segolodi, Kachit Choopanya, Jaray Tongtoyai, Timothy H Holtz, Taraz Samandari, Janet M Mcnicholl
Objective: The OraQuick Advance Rapid HIV-1/2 Test is a point-of-care test capable of detecting HIV-specific antibodies in blood and oral fluid. To understand test performance and factors contributing to false-negative (FN) results in longitudinal studies, we examined results of participants enrolled in the TDF2 study, the Bangkok Tenofovir Study, and the Bangkok MSM Cohort Study (BMCS), three separate clinical studies of high-risk, HIV-negative persons conducted in Botswana and Thailand...
March 20, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/28364412/the-validation-and-evaluation-of-anti-hiv-testing-algorithm-used-in-mobile-clinic-setting-for-men-who-have-sex-with-men-in-metropolitan-bangkok-thailand
#4
Tippawan Pankam, Sarun Saensiriphan, Suphackok Areeyolwattana, Napapat Barisri, Supabhorn Pengnonyang, Sunee Sirivichayakul, Nittaya Phanuphak, Praphan Phanuphak
BACKGROUND: Same-day anti-HIV testing algorithm is recommended by Thai National Guidelines. We compared performance characteristics of algorithms used in a mobile clinic and a facility-based clinic for men who have sex with men (MSM) in Bangkok. METHODS: Mobile clinic samples collected from 4 saunas and 2 spa venues were tested by Alere DetermineTM HIV 1/2, followed by DoubleCheck GoldTM Ultra HIV 1/2 and SD Bioline HIV 1/2 3.0. All samples were re-tested at the Thai Red Cross Anonymous Clinic (TRCAC) by Architect HIV Ag/Ab or Elecsys HIV combi PT, followed by Alere DetermineTM HIV 1/2 and Serodia HIV 1/2...
April 1, 2017: Asian Pacific Journal of Allergy and Immunology
https://www.readbyqxmd.com/read/28351689/detection-of-new-hiv-infections-in-a-multicentre-hiv-antiretroviral-pre-exposure-prophylaxis-trial
#5
Katrien Fransen, Irith de Baetselier, Elizabeth Rammutla, Khatija Ahmed, Frederick Owino, Walter Agingu, Gustav Venter, Jen Deese, Lut Van Damme, Tania Crucitti
BACKGROUND: Monthly specimens collected from FEM-PrEP-a Phase III trial [1] were investigated for the detection of acute HIV (AHI) infection. OBJECTIVES: To evaluate the efficiency of the study-specific HIV algorithm in detecting AHI, and the performance of each of the serological and molecular tests used in diagnosing new infections, and their contribution to narrowing the window period. STUDY DESIGN: A total of 83 pre-seroconversion specimens from 61 seroconverters from the FEM-PrEP trial were further analyzed in a sub-study...
March 20, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28317800/are-who-approved-nucleic-acid-amplification-tests-causing-large-scale-false-identification-of-rifampicin-resistant-tuberculosis-programmatic-experience-from-south-india
#6
Praveen Sanker, Anusree P Ambika, Vishnu T Santhosh, Ramya Puthukkudi Kottuthodi, Ravikrishnan Balakrishnan, Sunil Kumar Mrithunjayan, Hisham Moosan
INTRODUCTION: The nucleic acid amplification tests (NAATs): Line probe assay and GeneXpert (Xpert) have evolved as the primary tool for identification of rifampicin (RIF)-resistant (RR) tuberculosis (TB) worldwide, primarily because of the ease and speed. We rechecked RR isolates identified by NAATs from presumptive RR TB cases belonging to South India by the Revised National TB Control Program, India using multiple RIF concentrations on Bactec MGIT system and compared the mutation patterns with the resistance levels...
January 2017: International Journal of Mycobacteriology
https://www.readbyqxmd.com/read/28290950/survey-of-sexually-transmitted-disease-laboratory-methods-in-u-s-army-laboratories-2012
#7
Nicole K Leamer, Nikki N Jordan, Laura A Pacha, Nabil H Latif, Eric C Garges, Joel C Gaydos
INTRODUCTION: Sexually transmitted diseases (STD) affect primarily young people (17-24 years). The U.S. Military, with many young people, strives to maintain effective STD treatment and prevention programs using current methods. Laboratory testing technology and capacity are important for appropriate clinical management and to provide data to direct prevention programs. STD laboratory capabilities are assessed in civilian and military laboratories using surveys. An Army laboratory survey was conducted in 2007...
March 2017: Military Medicine
https://www.readbyqxmd.com/read/28283408/development-and-evaluation-of-an-enzyme-linked-immunosorbent-assay-for-the-detection-of-antibodies-to-a-common-urogenital-derivative-of-chlamydia-trachomatis-plasmid-encoded-pgp3
#8
Catherine E Winstanley, Kyle H Ramsey, Peter Marsh, Ian N Clarke
BACKGROUND: Urogenital infection with Chlamydia trachomatis is the most commonly diagnosed sexually transmitted infection in the developed world. Accurate measurement and therefore understanding the seroprevalence of urogenital C. trachomatis infections requires a rigorously optimised and validated ELISA. Previous ELISAs based on the C. trachomatis plasmid-encoded protein, PGP3, have been described but lack standardisation and critical controls or use a less common PGP3 as the capture antigen...
March 7, 2017: Journal of Immunological Methods
https://www.readbyqxmd.com/read/28275072/strategies-for-optimizing-the-diagnostic-predictive-value-of-clostridium-difficile-molecular-diagnostics
#9
Larry K Kociolek
Because nucleic acid amplification tests (NAATs) do not distinguish Clostridium difficile infection (CDI) and asymptomatic C. difficile carriage, the diagnostic predictive value of NAATs is limited when used in patients with low probability of CDI. In this issue of the Journal of Clinical Microbiology, Truong et al. report significant reductions in hospital-onset CDI and oral vancomycin utilization at their institution following implementation of a novel intervention that leveraged their clinical bioinformatics resources to prevent C...
March 8, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28245111/simplifying-nucleic-acid-amplification-from-whole-blood-with-direct-polymerase-chain-reaction-on-chitosan-microparticles
#10
Imaly A Nanayakkara, Weidong Cao, Ian M White
Tremendous advances have been made in the development of portable nucleic acid amplification devices for near-patient use. However, the true limitation in the realization of nucleic acid amplification tests (NAATs) for near-patient applications is not the amplification reaction, it is the complexity of the sample preparation. Conventional approaches require several precise intervention steps during the protocol. There are numerous reports in the literature that mimic the sample preparation procedure within a lab-on-a-chip device or cartridge, but these systems require a high number of integrated steps, making the devices and/or their supporting equipment too complex to meet the necessary cost targets and regulatory requirements for near-patient applications...
March 9, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28228485/probability-of-vertical-transmission-of-chlamydia-trachomatis-estimated-from-national-registry-data
#11
Minna Honkila, Erika Wikström, Marjo Renko, Heljä-Marja Surcel, Tytti Pokka, Irma Ikäheimo, Matti Uhari, Terhi Tapiainen
OBJECTIVES: Chlamydia trachomatis colonisation is common in pregnant women, and it has been claimed that mother-to-child transmission may occur in 10%-70% of deliveries. C. trachomatis infections are nevertheless rarely encountered in infants in clinical practice. In order to evaluate the reason for this discrepancy, we designed a nationwide study of the C. trachomatis vertical transmission. METHODS: Children with a possible C. trachomatis infection were identified from two national health registries in 1996-2011...
February 21, 2017: Sexually Transmitted Infections
https://www.readbyqxmd.com/read/28223147/comparative-performance-study-of-six-commercial-molecular-assays-for-rapid-detection-of-toxigenic-clostridium-difficile
#12
Yossi Paitan, Tamar Miller Roll, Amos Adler
OBJECTIVES: Rapid and accurate detection of C. difficile in stool impacts patient treatment and containment efforts. Detection of C. difficile toxin genes using nucleic acid amplification techniques (NAAT) is part of a multistep algorithm. Our objective was to directly compare the diagnostic accuracy and applicability of 6 commercial C. difficile NAAT assays. METHODS: In this study, 210 specimens were analysed in parallel by 6 commercial NAAT. Toxigenic culture was used as a reference method...
February 18, 2017: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/28199410/ultrasensitive-detection-of-hiv-1-p24-antigen-by-a-hybrid-nanomechanical-optoplasmonic-platform-with-potential-for-detecting-hiv-1-at-first-week-after-infection
#13
Priscila M Kosaka, Valerio Pini, Montserrat Calleja, Javier Tamayo
Early detection of HIV infection is the best way to prevent spread of the disease and to improve the efficiency of the antiretroviral therapy. Nucleic acid amplification tests (NAAT) have become the gold-standard for detecting low-concentrations of the virus in blood. However, these methods are technically demanding and cost-prohibitive in developing countries. Immunoassays are more affordable and can be more easily adapted for point-of-care diagnosis. However, the sensitivity so far of these methods has been too low...
2017: PloS One
https://www.readbyqxmd.com/read/28159916/impact-of-deploying-multiple-point-of-care-tests-with-a-sample-first-approach-on-a-sexual-health-clinical-care-pathway-a-service-evaluation
#14
Emma M Harding-Esch, Achyuta V Nori, Aseel Hegazi, Marcus J Pond, Olanike Okolo, Anthony Nardone, Catherine M Lowndes, Phillip Hay, S Tariq Sadiq
OBJECTIVES: To assess clinical service value of STI point-of-care test (POCT) use in a 'sample first' clinical pathway (patients providing samples on arrival at clinic, before clinician consultation). Specific outcomes were: patient acceptability; whether a rapid nucleic acid amplification test (NAAT) for Chlamydia trachomatis/Neisseria gonorrhoeae (CT/NG) could be used as a POCT in practice; feasibility of non-NAAT POCT implementation for Trichomonas vaginalis (TV) and bacterial vaginosis (BV); impact on patient diagnosis and treatment...
February 3, 2017: Sexually Transmitted Infections
https://www.readbyqxmd.com/read/28122873/rising-stakes-for-health-care-associated-infection-prevention-implications-for-the-clinical-microbiology-laboratory
#15
Daniel J Diekema
Health care-associated infection (HAI) rates are subject to public reporting and are linked to hospital reimbursement from the Centers for Medicare and Medicaid Services (CMS). The increasing pressure to lower HAI rates comes at a time when advances in the clinical microbiology laboratory (CML) provide more-precise and -sensitive tests, altering HAI detection in ways that may increase reported HAI rates. I review how changing CML practices can impact HAI rates and how the financial implications of HAI metrics may produce pressure to change diagnostic testing practices...
April 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28077697/point-counterpoint-what-is-the-optimal-approach-for-detection-of-clostridium-difficile-infection
#16
Ferric C Fang, Christopher R Polage, Mark H Wilcox
INTRODUCTIONIn 2010, we published an initial Point-Counterpoint on the laboratory diagnosis of Clostridium difficile infection (CDI). At that time, nucleic acid amplification tests (NAATs) were just becoming commercially available, and the idea of algorithmic approaches to CDI was being explored. Now, there are numerous NAATs in the marketplace, and based on recent proficiency test surveys, they have become the predominant method used for CDI diagnosis in the United States. At the same time, there is a body of literature that suggests that NAATs lack clinical specificity and thus inflate CDI rates...
March 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28077695/stability-studies-on-dry-swabs-and-wet-mailed-swabs-for-detection-of-chlamydia-trachomatis-and-neisseria-gonorrhoeae-in-aptima-assays
#17
Jeanne Moncada, Carey B Clark, Jeffrey Holden, Edward W Hook, Charlotte A Gaydos, Julius Schachter
The Aptima Combo 2 (AC2) and Aptima CT (ACT) (Hologic Inc., San Diego, CA) are nucleic acid amplification tests (NAATs) that detect Chlamydia trachomatis AC2 also detects Neisseria gonorrhoeae Storage and temperature conditions may impact the utility of NAATs in some settings and screening programs. We evaluated specimen stability for use beyond the Aptima package insert specifications for temperature and duration of storage (between 2°C and 30°C and 60 days, respectively) in two studies: (i) dry C. trachomatis-seeded swabs were used with ACT after storage at 4°C, 23°C, or 36°C for up to 84 days and (ii) swabs seeded with C...
March 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28076298/comparison-of-abbott-realtime-genotype-ii-genematrix-restriction-fragment-mass-polymorphism-and-sysmex-hiscl-hcv-gr-assays-for-hepatitis-c-virus-genotyping
#18
Mi-Soon Han, Yongjung Park, Hyon-Suk Kim
BACKGROUND: Hepatitis C virus (HCV) genotype is a predictive marker for treatment response. We sequentially evaluated the performances of two nucleic acid amplification tests (NAATs) and one serology assay for HCV genotype: Abbott RealTime genotype II (RealTime II), GeneMatrix restriction fragment mass polymorphism (RFMP), and Sysmex HISCL HCV Gr (HISCL Gr). METHODS: We examined 281 clinical samples with three assays. The accuracy was assessed using the HCV Genotype Performance Panel PHW204 (SeraCare Life Sciences) for two NAATs...
January 11, 2017: Clinical Chemistry and Laboratory Medicine: CCLM
https://www.readbyqxmd.com/read/28055571/a-review-of-methods-for-detection-of-hepatozoon-infection-in-carnivores-and-arthropod-vectors
#19
David Modrý, Relja Beck, Kristýna Hrazdilová, Gad Baneth
Vector-borne protists of the genus Hepatozoon belong to the apicomplexan suborder Adeleorina. The taxonomy of Hepatozoon is unsettled and different phylogenetic clades probably represent evolutionary units deserving the status of separate genera. Throughout our review, we focus on the monophyletic assemblage of Hepatozoon spp. from carnivores, classified as Hepatozoon sensu stricto that includes important pathogens of domestic and free-ranging canine and feline hosts. We provide an overview of diagnostic methods and approaches from classical detection in biological materials, through serological tests to nucleic acid amplification tests (NAATs)...
January 2017: Vector Borne and Zoonotic Diseases
https://www.readbyqxmd.com/read/28053213/calibration-of-bk-virus-nucleic-acid-amplification-testing-to-the-1st-who-international-standard-for-bk-virus
#20
Susanna K Tan, Stephen Milligan, Malaya K Sahoo, Nathaniel Taylor, Benjamin A Pinsky
Significant interassay variability in the quantification of BK virus (BKV) DNA precludes establishing broadly applicable thresholds for the management of BKV infection in transplantation. The 1st WHO International Standard for BKV (primary standard) was introduced in 2016 as a common calibrator for improving the harmonization of BKV nucleic acid amplification testing (NAAT) and enabling comparisons of biological measurements worldwide. Here, we evaluated the Altona RealStar BKV assay (Altona) and calibrated the results to the international unit (IU) using the Exact Diagnostics BKV verification panel, a secondary standard traceable to the primary standard...
March 2017: Journal of Clinical Microbiology
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