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https://www.readbyqxmd.com/read/28445867/the-mir-106a-363xpcl1-mirna-cluster-induces-murine-t-cell-lymphoma-despite-transcriptional-activation-of-the-p27kip1-cell-cycle-inhibitor
#1
Daniel A Kuppers, Thomas M Schmitt, Harry C Hwang, Lavanya Samraj, Bruce E Clurman, Matthew L Fero
The miR-106a~363 cluster encodes 6 miRNAs on the X-chromosome which are abundant in blood cells and overexpressed in a variety of malignancies. The constituent miRNA of miR-106a~363 have functional activities in vitro that are predicted to be both oncogenic and tumor suppressive, yet little is known about their physiological functions in vivo. Mature miR-106a~363 (Mirc2) miRNAs are processed from an intragenic, non-protein encoding gene referred to as Xpcl1 (or Kis2), situated at an X-chromosomal locus frequently targeted by retroviruses in murine lymphomas...
April 7, 2017: Oncotarget
https://www.readbyqxmd.com/read/28445864/tumor-antigen-glycosaminoglycan-modification-regulates-antibody-drug-conjugate-delivery-and-cytotoxicity
#2
Helena C Christianson, Julien A Menard, Vineesh Indira Chandran, Erika Bourseau-Guilmain, Dmitry Shevela, Jon Lidfeldt, Ann-Sofie Månsson, Silvia Pastorekova, Johannes Messinger, Mattias Belting
Aggressive cancers are characterized by hypoxia, which is a key driver of tumor development and treatment resistance. Proteins specifically expressed in the hypoxic tumor microenvironment thus represent interesting candidates for targeted drug delivery strategies. Carbonic anhydrase (CAIX) has been identified as an attractive treatment target as it is highly hypoxia specific and expressed at the cell-surface to promote cancer cell aggressiveness. Here, we find that cancer cell internalization of CAIX is negatively regulated by post-translational modification with chondroitin or heparan sulfate glycosaminoglycan chains...
April 7, 2017: Oncotarget
https://www.readbyqxmd.com/read/28445854/balance-training-in-individuals-with-parkinson-s-disease-therapist-supervised-vs-home-based-exercise-programme
#3
Elizabeth Maria Atterbury, Karen Estelle Welman
BACKGROUND: Poor locomotion and balance in Parkinson's disease (PD) often diminishes independence. Accordingly, gait is considered one of the most relevant rehabilitation outcomes, and home-based balance exercises might be a viable mode of exercise delivery for individuals with PD. However, research on PD interventions rarely indicate best practices to deliver exercises. Therefore, this study endeavoured to compare the efficacy of a home-based and therapist-supervised balance programme on gait parameters, dynamic balance, balance confidence and motivation in individuals diagnosed with PD...
April 4, 2017: Gait & Posture
https://www.readbyqxmd.com/read/28445827/scale-effect-on-spatial-patterns-of-ecosystem-services-and-associations-among-them-in-semi-arid-area-a-case-study-in-ningxia-hui-autonomous-region-china
#4
Shuna Xu, Yanfang Liu, Xia Wang, Guangxia Zhang
Identifying scale effect on spatial patterns of ecosystem services and associations among them has been recognized as critical to the sustainable management of ecosystem services. We proposed a method to conveniently integrate ecosystem services of small scale into different larger scales. Taking Ningxia Hui Autonomous Region in west China as an example, we analyzed the change regularity of spatial patterns of 7 ecosystem services under 22 different scales. Further, the tradeoffs and synergies among ecosystem services across all scales were compared...
April 23, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28445772/rosiglitazone-pretreatment-influences-thrombin-induced-phagocytosis-by-rat-microglia-via-activating-ppar%C3%AE-and-cd36
#5
Qiong Mu, Likun Wang, Hang Hang, Chunfeng Liu, Guofeng Wu
OBJECTIVE: To explore the protective role of rosiglitazone against secondary brain injury after cerebral hemorrhage, we investigated the effect of rosiglitazone pretreatment on thrombin-induced microglial phagocytosis and described the molecular mechanisms involved in this process. METHODS: Primary microglial cells were obtained from the brain tissue of newborn Sprague-Dawley rats and were randomly divided into four groups: the normal, thrombin stimulation, thrombin-treated plus rosiglitazone, and thrombin-rosiglitazone plus proliferator-activated receptor-gamma (PPARγ) antagonist groups...
April 23, 2017: Neuroscience Letters
https://www.readbyqxmd.com/read/28445749/equilibrium-ensembles-for-insulin-folding-from-bias-exchange-metadynamics
#6
Richa Singh, Rohit Bansal, Anurag Singh Rathore, Gaurav Goel
Earliest events in the aggregation process, such as single molecule reconfiguration, are extremely important and the most difficult to characterize in experiments. To this end, we have used well-tempered bias exchange metadynamics simulations to determine the equilibrium ensembles of an insulin molecule under amyloidogenic conditions of low pH and high temperature. A bin-based clustering method that uses statistics accumulated in bias exchange metadynamics trajectories was employed to construct a detailed thermodynamic and kinetic model of insulin folding...
April 25, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/28445684/patient-staffing-and-health-centre-factors-associated-with-annual-testing-for-sexually-transmissible-infections-in-remote-primary-health-centres
#7
Belinda Hengel, Handan Wand, James Ward, Alice Rumbold, Linda Garton, Debbie Taylor-Thomson, Bronwyn Silver, Skye McGregor, Amalie Dyda, Jacqueline Mein, Janet Knox, Lisa Maher, John Kaldor, Rebecca Guy
Background: In high-incidence Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) settings, annual re-testing is an important public health strategy. Using baseline laboratory data (2009-10) from a cluster randomised trial in 67 remote Aboriginal communities, the extent of re-testing was determined, along with the associated patient, staffing and health centre factors. Methods: Annual testing was defined as re-testing in 9-15 months (guideline recommendation) and a broader time period of 5-15 months following an initial negative CT/NG test...
April 27, 2017: Sexual Health
https://www.readbyqxmd.com/read/28445543/comparative-genomic-analysis-between-corynebacterium-pseudotuberculosis-strains-isolated-from-buffalo
#8
Marcus Vinicius Canário Viana, Henrique Figueiredo, Rommel Ramos, Luis Carlos Guimarães, Felipe Luiz Pereira, Fernanda Alves Dorella, Salah Abdel Karim Selim, Mohammad Salaheldean, Artur Silva, Alice R Wattam, Vasco Azevedo
Corynebacterium pseudotuberculosis is a Gram-positive, pleomorphic, facultative intracellular pathogen that causes Oedematous Skin Disease (OSD) in buffalo. To better understand the pathogenic mechanisms of OSD, we performed a comparative genomic analysis of 11 strains of C. pseudotuberculosis isolated from different buffalo found to be infected in Egypt during an outbreak that occurred in 2008. Sixteen previously described pathogenicity islands (PiCp) were present in all of the new buffalo strains, but one of them, PiCp12, had an insertion that contained both a corynephage and a diphtheria toxin gene, both of which may play a role in the adaptation of C...
2017: PloS One
https://www.readbyqxmd.com/read/28445532/use-of-molecular-markers-in-identification-and-characterization-of-resistance-to-rice-blast-in-india
#9
Manoj Kumar Yadav, Aravindan S, Umakanta Ngangkham, H N Shubudhi, Manas Kumar Bag, Totan Adak, Sushmita Munda, Sanghamitra Samantaray, Mayabini Jena
Rice blast disease caused by Magnaporthe oryzae is one of the most destructive disease causing huge losses to rice yield in different parts of the world. Therefore, an attempt has been made to find out the resistance by screening and studying the genetic diversity of eighty released rice varieties by National Rice Research Institute, Cuttack (NRVs) using molecular markers linked to twelve major blast resistance (R) genes viz Pib, Piz, Piz-t, Pik, Pik-p, Pikm Pik-h, Pita/Pita-2, Pi2, Pi9, Pi1 and Pi5. Out of which, nineteen varieties (23...
2017: PloS One
https://www.readbyqxmd.com/read/28445524/live-imaging-of-primary-ocular-vasculature-formation-in-zebrafish
#10
Tetsuya Hashiura, Eiji Kimura, Shizuko Fujisawa, Sayuri Oikawa, Shigenori Nonaka, Daijiro Kurosaka, Jiro Hitomi
Ocular vasculature consists of the central retinal and ciliary vascular systems, which are essential to maintaining visual function. Many researchers have attempted to determine their origins and development; however, the detailed, stepwise process of ocular vasculature formation has not been established. In zebrafish, two angioblast clusters, the rostral and midbrain organizing centers, form almost all of the cranial vasculature, including the ocular vasculature, and these are from where the cerebral arterial and venous angioblast clusters, respectively, differentiate...
2017: PloS One
https://www.readbyqxmd.com/read/28445496/a-fuzzy-co-clustering-algorithm-for-biomedical-data
#11
Yongli Liu, Shuai Wu, Zhizhong Liu, Hao Chao
Fuzzy co-clustering extends co-clustering by assigning membership functions to both the objects and the features, and is helpful to improve clustering accurarcy of biomedical data. In this paper, we introduce a new fuzzy co-clustering algorithm based on information bottleneck named ibFCC. The ibFCC formulates an objective function which includes a distance function that employs information bottleneck theory to measure the distance between feature data point and the feature cluster centroid. Many experiments were conducted on five biomedical datasets, and the ibFCC was compared with such prominent fuzzy (co-)clustering algorithms as FCM, FCCM, RFCC and FCCI...
2017: PloS One
https://www.readbyqxmd.com/read/28445471/a-randomized-trial-comparing-concise-and-standard-consent-forms-in-the-start-trial
#12
Christine Grady, Giota Touloumi, A Sarah Walker, Mary Smolskis, Shweta Sharma, Abdel G Babiker, Nikos Pantazis, Jorge Tavel, Eric Florence, Adriana Sanchez, Fleur Hudson, Antonios Papadopoulos, Ezekiel Emanuel, Megan Clewett, David Munroe, Eileen Denning
BACKGROUND: Improving the effectiveness and efficiency of research informed consent is a high priority. Some express concern about longer, more complex, written consent forms creating barriers to participant understanding. A recent meta-analysis concluded that randomized comparisons were needed. METHODS: We conducted a cluster-randomized non-inferiority comparison of a standard versus concise consent form within a multinational trial studying the timing of starting antiretroviral therapy in HIV+ adults (START)...
2017: PloS One
https://www.readbyqxmd.com/read/28445434/an-adaptive-clustering-approach-based-on-minimum-travel-route-planning-for-wireless-sensor-networks-with-a-mobile-sink
#13
Jiqiang Tang, Wu Yang, Lingyun Zhu, Dong Wang, Xin Feng
In recent years, Wireless Sensor Networks with a Mobile Sink (WSN-MS) have been an active research topic due to the widespread use of mobile devices. However, how to get the balance between data delivery latency and energy consumption becomes a key issue of WSN-MS. In this paper, we study the clustering approach by jointly considering the Route planning for mobile sink and Clustering Problem (RCP) for static sensor nodes. We solve the RCP problem by using the minimum travel route clustering approach, which applies the minimum travel route of the mobile sink to guide the clustering process...
April 26, 2017: Sensors
https://www.readbyqxmd.com/read/28445428/residue-modification-and-mass-spectrometry-for-the-investigation-of-structural-and-metalation-properties-of-metallothionein-and-cysteine-rich-proteins
#14
REVIEW
Gordon W Irvine, Martin J Stillman
Structural information regarding metallothioneins (MTs) has been hard to come by due to its highly dynamic nature in the absence of metal-thiolate cluster formation and crystallization difficulties. Thus, typical spectroscopic methods for structural determination are limited in their usefulness when applied to MTs. Mass spectrometric methods have revolutionized our understanding of protein dynamics, structure, and folding. Recently, advances have been made in residue modification mass spectrometry in order to probe the hard-to-characterize structure of apo- and partially metalated MTs...
April 26, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28445411/discovery-of-farnesoid-x-receptor-antagonists-based-on-a-library-of-oleanolic-acid-3-o-esters-through-diverse-substituent-design-and-molecular-docking-methods
#15
Shao-Rong Wang, Tingting Xu, Kai Deng, Chi-Wai Wong, Jinsong Liu, Wei-Shuo Fang
The pentacyclic triterpene oleanolic acid (OA, 1) with known farnesoid X receptor (FXR) modulatory activity was modified at its C-3 position to find new FXR-interacting agents. A diverse substitution library of OA derivatives was constructed in silico through a 2D fingerprint similarity cluster strategy. With further docking analysis, four top-scored OA 3-O-ester derivatives were selected for synthesis. The bioassay results indicated that all four compounds 3 inhibited chenodeoxycholic acid (CDCA)-induced FXR transactivation in a concentration-dependent mode...
April 26, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28445293/possible-pathways-used-to-predict-different-stages-of-lung-adenocarcinoma
#16
Xiaodong Chen, Qiongyu Duan, Ying Xuan, Yunan Sun, Rong Wu
We aimed to find some specific pathways that can be used to predict the stage of lung adenocarcinoma.RNA-Seq expression profile data and clinical data of lung adenocarcinoma (stage I [37], stage II 161], stage III [75], and stage IV [45]) were obtained from the TCGA dataset. The differentially expressed genes were merged, correlation coefficient matrix between genes was constructed with correlation analysis, and unsupervised clustering was carried out with hierarchical clustering method. The specific coexpression network in every stage was constructed with cytoscape software...
April 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28445259/the-role-of-stat3-and-ahr-in-the-differentiation-of-cd4-t-cells-into-th17-and-treg-cells
#17
Xingxing Liu, Hui Hu, Heng Fan, Dongmei Zuo, Zhexing Shou, Yi Liao, Zhen Nan, Qing Tang
BACKGROUND: This study aimed to investigate the role of aryl hydrocarbon receptor (AhR) and STAT3 gene during the differentiation of cluster of differentiation (CD)4 T cells into T helper (Th)17 and T regulatory (Treg) cells. METHODS: First, CD4 T cells were isolated from the spleen of BALB/c mice. Then, stable CD4 T cells expressing STAT3 shRNA were constructed. CD4 T cells were assigned to one of the following treatments: Th17 group: antibodies against CD3 and CD28, 2...
April 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28445054/catalytic-decomposition-of-no-by-cationic-platinum-oxide-cluster-pt3o4
#18
Jun Yamagishi, Ken Miyajima, Satoshi Kudoh, Fumitaka Mafune
The catalytic decomposition of NO by cationic platinum oxide cluster Pt3O4+ was investigated by mass spectrometry and thermal desorption spectrometry. Upon reaction with two NO molecules, molecular oxygen desorbed from the cluster at room temperature to form Pt3O4N2+. Then, at temperatures above 400 K, desorption of N2 from Pt3O4N2+ was observed. These processes were confirmed by isotope-labeling experiments, and the energetics of O2 and N2 release were determined by density functional calculations. The combination of these elementary steps resulted in the catalytic decomposition of NO by Pt3O4+...
April 26, 2017: Journal of Physical Chemistry Letters
https://www.readbyqxmd.com/read/28445004/cftr-dependent-chloride-efflux-in-cystic-fibrosis-mononuclear-cells-is-increased-by-ivacaftor-therapy
#19
Lorenzo Guerra, Susanna D'Oria, Maria Favia, Stefano Castellani, Teresa Santostasi, Angela M Polizzi, Maria A Mariggiò, Crescenzio Gallo, Valeria Casavola, Pasqualina Montemurro, Giuseppina Leonetti, Antonio Manca, Massimo Conese
AIM: The Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) potentiator ivacaftor (Kalydeco®) improves clinical outcome in G551D cystic fibrosis (CF) patients. Here, we have investigated whether ivacaftor has a clinical impact on non-G551D gating mutations and function of circulating leukocytes as well. METHODS: Seven patients were treated with ivacaftor and evaluated at baseline, and at 1-3 and 6 months. Besides clinical and systemic inflammatory parameters, circulating mononuclear cells (MNC) were evaluated for CFTR-dependent chloride efflux by spectrofluorimetry, neutrophils for oxidative burst by cytofluorimetry and HVCN1 mRNA expression by real time PCR...
April 26, 2017: Pediatric Pulmonology
https://www.readbyqxmd.com/read/28444973/in-depth-comparative-phenotyping-of-blood-innate-myeloid-leukocytes-from-healthy-humans-and-macaques-using-mass-cytometry
#20
Jamila Elhmouzi-Younes, Jean-Louis Palgen, Nicolas Tchitchek, Simon Delandre, Inana Namet, Caroline L Bodinham, Kathleen Pizzoferro, David J M Lewis, Roger Le Grand, Antonio Cosma, Anne-Sophie Beignon
Comparative immune-profiling of innate responses in humans and non-human primates is important to understand the pathogenesis of infectious and chronic inflammatory diseases as well as for the preclinical development of vaccines and immune therapies. However, direct comparisons of the two species are rare and were never performed using mass cytometry. Here, whole-blood-derived leukocytes from healthy humans and cynomolgus macaques were analyzed with mass cytometry. Two similar panels of around 30 monoclonal antibodies targeting human markers associated with innate myeloid cells to stain fixed human and macaque leukocytes were constructed...
April 26, 2017: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
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