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https://www.readbyqxmd.com/read/29917328/live-cell-imaging-by-confocal-raman-and-fluorescence-microscopy-recognizes-the-crystal-structure-of-calcium-carbonate-particles-in-hela-cells
#1
Аnatoly A Abalymov, Roman A Verhovskiy, Marina V Novoselova, Bogdan V Parakhonskiy, Dmitry A Gorin, Alexey M Yashchenok, Gleb B Sukhorukov
Porous calcium carbonate (CaCO3 ) vaterite particles are very attractive templates for the encapsulation of pharmaceuticals and for the construction of hollow polyelectrolyte capsules, sensors, and enzyme-catalyzed reactors. Although CaCO3 is biocompatible and biodegradable, little is known about the intercellular behavior and properties of vaterite particles in the cytoplasm of cells. In this work, we combined confocal Raman and fluorescent microscopy for the imaging of porous CaCO3 vaterite particles in HeLa cells to study the uptake and status of the particles inside the cells in real time...
June 19, 2018: Biotechnology Journal
https://www.readbyqxmd.com/read/29917307/purification-of-uranium-based-endohedral-metallofullerenes-emfs-by-selective-supramolecular-encapsulation-and-release
#2
Carles Fuertes-Espinosa, Alejandra Gómez-Torres, Roser Morales-Martínez, Antonio Rodríguez-Fortea, Cristina García-Simón, Felipe Gándara, Inhar Imaz, Judith Juanhuix, Daniel Maspoch, Josep M Poblet, Luis Echegoyen, Xavi Ribas
Supramolecular nanocapsule 1·(BArF)8 is able to sequentially and selectively entrap recently discovered U2@C80 and unprecedented Sc2CU@C80, simply by soaking crystals of 1·(BArF)8 in a toluene solution of arc-produced soot. These species, selectively and stepwise absorbed by 1·(BArF)8, are easily released, obtaining highly pure fractions of U2@C80 and Sc2CU@C80 in one step. Sc2CU@C80 represents the first example of a mixed metal actinide-based endohedral metallofullerene (EMF). Remarkably, the host-guest studies revealed that 1·(BArF)8 is able to discriminate EMFs with the same carbon cage but with different encapsulated cluster and computational studies provide support for these observations...
June 19, 2018: Angewandte Chemie
https://www.readbyqxmd.com/read/29917284/quantum-simulation-verifies-the-stability-of-an-18-coordinated-actinium-helium-complex
#3
Eiki Ozama, Sadia Adachi, Toshiyuki Takayanagi, Motoyuki Shiga
The structures of trivalent actinium cation in helium clusters (Ac3+Hen) have been studied by quantum path integral molecular dynamics simulations with different cluster sizes, n = 18-200. The nuclear quantum effect of helium atoms plays an important role in the vibrational amplitude of the Ac3+-He complex at low temperatures (1-3 K) where the complex is stable. We found that the coordination number of helium atoms comprising the first solvation shell can be as high as eighteen. In this case, the helium atoms are arranged in D4d symmetry...
June 19, 2018: Chemistry: a European Journal
https://www.readbyqxmd.com/read/29917235/classification-of-gabaergic-neuron-subtypes-from-the-globus-pallidus-using-wild-type-and-transgenic-mice
#4
Karina P Abrahao, David M Lovinger
KEY POINTS: Classifying different subtypes of neurons in deep brain structures is a challenge and is crucial to better understanding brain function. Understanding the diversity of neurons in the Globus Pallidus, a brain region positioned to influence afferent and efferent information processing within basal ganglia, could help to explain a variety of brain functions. We present a classification of neurons from the GP using electrophysiological data from wild type mice and confirmation using transgenic mice...
June 19, 2018: Journal of Physiology
https://www.readbyqxmd.com/read/29917197/crispr-technology-new-paradigm-to-target-the-infectious-disease-pathogens
#5
M A Bakhrebah, M S Nassar, M S Alsuabeyl, W A Zaher, S A Meo
OBJECTIVE: Infectious diseases are one of the prime causes of death worldwide. An innovative sequence specific editing technology "Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)" has been tested on a broad range of microorganisms to target and destroy invading foreign DNA to human cells or tissues. This study aimed to discuss the mechanism and therapeutic usage of CRISPR/Cas9 genome editing technology in the management of various infectious disease pathogens...
June 2018: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29917168/evaluation-of-anticancer-agents-using-patient-derived-tumor-organoids-characteristically-similar-to-source-tissues
#6
Hirosumi Tamura, Arisa Higa, Hirotaka Hoshi, Gen Hiyama, Nobuhiko Takahashi, Masae Ryufuku, Gaku Morisawa, Yuka Yanagisawa, Emi Ito, Jun-Ichi Imai, Yuu Dobashi, Kiyoaki Katahira, Shu Soeda, Takafumi Watanabe, Keiya Fujimori, Shinya Watanabe, Motoki Takagi
Patient-derived tumor xenograft models represent a promising preclinical cancer model that better replicates disease, compared with traditional cell culture; however, their use is low-throughput and costly. To overcome this limitation, patient-derived tumor organoids (PDOs) were established from human lung, ovarian and uterine tumor tissues, among others, to accurately and efficiently recapitulate the tissue architecture and function. PDOs were able to be cultured for >6 months, and formed cell clusters with similar morphologies to their source tumors...
June 18, 2018: Oncology Reports
https://www.readbyqxmd.com/read/29917155/molecular-characteristic-and-pathogenicity-analysis-of-a-virulent-recombinant-avain-infectious-bronchitis-virus-isolated-in-china
#7
K Y Feng, T Chen, X Zhang, G M Shao, Y Cao, D K Chen, W C Lin, F Chen, Q M Xie
A virulent infectious bronchitis virus (IBV), designated as CK/CH/GD/QY16 (referred as QY16), was isolated from a diseased chicken farm in Guangdong province, China, in 2016. The complete genome of the strain was sequenced and analyzed. The results show that the genome of QY16 consists of 27,670 nucleotides, excluding poly (A) tail, and that its genome organization is 5' UTR-1a-1b-S-3a-3b-E-M-4b-4c-5a-5b-N-6b-3' UTR-poly (A) tail. Sequence comparison among QY16 and other IBV strains was conducted and its results demonstrate that the S1 gene of QY16 has the highest nucleotide sequence identity with that of 4/91, and the other part of its genome is highly similar to that of YX10...
June 15, 2018: Poultry Science
https://www.readbyqxmd.com/read/29917154/improving-the-safety-climate-in-hospitals-by-a-vignette-based-analysis-of-adverse-events-a-cluster-randomised-study
#8
Pauline Occelli, Jean-Luc Quenon, Marion Kret, Sandrine Domecq, Angélique Denis, Florence Delaperche, Olivier Claverie, Benjamin Castets-Fontaine, René Amalberti, Yves Auroy, Pierre Parneix, Philippe Michel
Objective: To assess the impact of a vignette-based analysis of adverse events (AEs) on the safety climate (SC) of care units. Design: Prospective, open, cluster (a unit) randomised controlled trial. Setting: Eighteen acute care units of seven hospitals in France. Participants: Healthcare providers who worked in the units. Intervention: Vignette-based analyses of AEs were conducted with unit's providers once per month for six consecutive months...
June 18, 2018: International Journal for Quality in Health Care
https://www.readbyqxmd.com/read/29917151/synthesis-of-seed-specific-bidirectional-promoters-for-metabolic-engineering-of-anthocyanin-rich-maize
#9
Xiaoqing Liu, Suzhen Li, Wenzhu Yang, Bona Mu, Yong Jiao, Xiaojin Zhou, Chunyi Zhang, Yunliu Fan, Rumei Chen
Tissue-specific promoters play an important role in plant molecular farming. Here, we describe a strategy to modify the tissue specificity of a maize embryo-specific bidirectional promoter PZmBD1. Six types of cis elements: RY repeats (R), GCN4 (G), the prolamin box (P), Skn-1 (S), and the ACGT and AACA (A) motifs, were collected and fused to PZmBD1 to generate eight chimeric putative bidirectional promoters. Qualitative and quantitative analysis of reporter genes driven by the promoters showed that two promoters exhibited high seed-specific bidirectional activity in maize transient and stable transformed system...
June 15, 2018: Plant & Cell Physiology
https://www.readbyqxmd.com/read/29917147/the-feasibility-and-acceptability-of-mass-drug-administration-for-malaria-in-cambodia-a-mixed-methods-study
#10
Thomas J Peto, Rupam Tripura, Nou Sanann, Bipin Adhikari, James Callery, Mark Droogleever, Chhouen Heng, Phaik Yeong Cheah, Chan Davoeung, Chea Nguon, Lorenz von Seidlein, Arjen M Dondorp, Christopher Pell
Background: Mass drug administrations (MDAs) are part of the World Health Organization's Plasmodium falciparum elimination strategy for the Greater Mekong Subregion (GMS). In Cambodia, a 2015-2017 clinical trial evaluated the effectiveness of MDA. This article explores factors that influence the feasibility and acceptability of MDA, including seasonal timing, financial incentives and the delivery model. Methods: Quantitative data were collected through structured questionnaires from the heads of 163 households...
June 16, 2018: Transactions of the Royal Society of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/29917129/seroprevalence-of-dengue-in-school-children-in-mexico-ages-6-17-years-2016
#11
Irma Y Amaya-Larios, Mario Rojas-Russell, Malaquias López-Cervantes, Lilia Castro-Porras, Ma Victoria Castro-Borbonio, Elsa Sarti, Esteban Puentes-Rosas, Laura L Tirado-Gómez, Gustavo Olaíz-Fernandez, José Ramos-Castañeda
Background: Dengue is the most important arboviral disease in the world. Seroprevalence has been proposed as a marker of endemicity, however, studies are scarce. Methods: We conducted a cross-sectional, stratified cluster, random sample study to measure the seroprevalence of antibodies to dengue virus (DENV) in Mexico. The target population was school children ages 6-17 y from 22 endemic states in Mexico, clustered in four regions: Pacific, South-Central, Southeast and Low...
June 16, 2018: Transactions of the Royal Society of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/29917057/a-bayesian-space-time-model-for-clustering-areal-units-based-on-their-disease-trends
#12
Gary Napier, Duncan Lee, Chris Robertson, Andrew Lawson
Population-level disease risk across a set of non-overlapping areal units varies in space and time, and a large research literature has developed methodology for identifying clusters of areal units exhibiting elevated risks. However, almost no research has extended the clustering paradigm to identify groups of areal units exhibiting similar temporal disease trends. We present a novel Bayesian hierarchical mixture model for achieving this goal, with inference based on a Metropolis-coupled Markov chain Monte Carlo ((MC)$^3$) algorithm...
June 18, 2018: Biostatistics
https://www.readbyqxmd.com/read/29917039/aetiological-characterization-of-the-cutaneous-ulcer-syndrome-in-papua-new-guinea-using-shotgun-metagenomics
#13
Marc Noguera-Julian, Camila González-Beiras, Mariona Parera, August Kapa, Roger Paredes, Oriol Mitjà
Background: Treponema pallidum subsp. pertenue and Haemophilus ducreyi are causative agents of cutaneous ulcer (CU) in yaws endemic regions in the tropics. However, a significant proportion of CU patients remain PCR-negative for both bacterial agents. We aimed to identify potential additional aetiological agents of CU in a yaws-endemic region. Methods: This population-based cohort study included children in Lihir Island (Papua New Guinea) examined during a yaws eradication campaign in Oct 2013-Oct 2014...
June 16, 2018: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/29916994/uric-acid-is-linked-to-cardiometabolic-risk-factors-in-overweight-and-obese-youths
#14
Empar Lurbe, María Isabel Torro, Julio Alvarez-Pitti, Josep Redon, Claudio Borghi, Pau Redon
OBJECTIVE: Observational studies have indicated that high levels of serum uric acid are associated with the risk of cardiovascular disease. The aim of the present study is to investigate the association of uric acid with individual cardiometabolic risk factors, as well as their degree of clustering, in overweight and moderate obese youth. METHODS: Three hundred and thirty-three Caucasians of both sexes (149 women), from 5-18 years of age from those who underwent an assessment of overweight/obesity...
June 18, 2018: Journal of Hypertension
https://www.readbyqxmd.com/read/29916820/density-functional-tight-binding-approach-for-metal-clusters-nanoparticles-surfaces-and-bulk-application-to-silver-and-gold
#15
Jérôme Cuny, Nathalie Tarrat, Fernand Spiegelman, Arthur Huguenot, Mathias Rapacioli
Density-functional based tight-binding (DFTB) is an efficient quantum mechanical method that can describe a variety of systems, going from organic and inorganic compounds to metallic and hybrid materials. The present topical review addresses the ability and performance of DFTB to investigate energetic, structural, spectroscopic and dynamical properties of gold and silver materials. After a brief overview of the theoretical basis of DFTB, its parametrization and its transferability, we report its past and recent applications to gold and silver systems, including small clusters, nanoparticles, bulks and surfaces, bare and interacting with various organic and inorganic compounds...
June 19, 2018: Journal of Physics. Condensed Matter: An Institute of Physics Journal
https://www.readbyqxmd.com/read/29916797/clermontyping-an-easy-to-use-and-accurate-in-silico-method-for-escherichia-genus-strain-phylotyping
#16
Johann Beghain, Antoine Bridier-Nahmias, Hervé Le Nagard, Erick Denamur, Olivier Clermont
The genus Escherichia is composed of Escherichia albertii, E. fergusonii, five cryptic Escherichia clades and E. coli sensu stricto. Furthermore, the E. coli species can be divided into seven main phylogroups termed A, B1, B2, C, D, E and F. As specific lifestyles and/or hosts can be attributed to these species/phylogroups, their identification is meaningful for epidemiological studies. Classical phenotypic tests fail to identify non-sensu stricto E. coli as well as phylogroups. Clermont and colleagues have developed PCR assays that allow the identification of most of these species/phylogroups, the triplex/quadruplex PCR for E...
June 19, 2018: Microbial Genomics
https://www.readbyqxmd.com/read/29916546/characteristics-and-multi%C3%A2-lineage-differentiation-of-bone-marrow-mesenchymal-stem-cells-derived-from-the-tibetan-mastiff
#17
Shuang Zhang, Chenqiong Zhao, Shi Liu, Yufeng Wang, Yuhua Zhao, Weijun Guan, Zhiqiang Zhu
Bone marrow mesenchymal stem cells (BM‑MSCs) are pluripotent stem cells that are regarded as ideal resources for the reconstruction of tissues and organs. The Tibetan mastiff is a breed of domesticated Chinese native dog that is well‑adjusted to the high‑altitude environments of Tibet. To the best of our knowledge, the biological characterization and multi‑lineage differentiation of Tibetan mastiff BM‑MSCs have not been reported previously. Therefore, the present study aimed to investigate the biological characteristics and therapeutic potential of Tibetan mastiff BM‑MSCs...
June 15, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29916519/natural-products-from-thioester-reductase-containing-biosynthetic-pathways
#18
REVIEW
Michael W Mullowney, Ryan A McClure, Matthew T Robey, Neil L Kelleher, Regan J Thomson
Covering: up to 2018Thioester reductase domains catalyze two- and four-electron reductions to release natural products following assembly on nonribosomal peptide synthetases, polyketide synthases, and their hybrid biosynthetic complexes. This reductive off-loading of a natural product yields an aldehyde or alcohol, can initiate the formation of a macrocyclic imine, and contributes to important intermediates in a variety of biosyntheses, including those for polyketide alkaloids and pyrrolobenzodiazepines. Compounds that arise from reductase-terminated biosynthetic gene clusters are often reactive and exhibit biological activity...
June 19, 2018: Natural Product Reports
https://www.readbyqxmd.com/read/29916512/tuning-the-affinity-of-catechols-and-salicylic-acids-towards-al-iii-characterization-of-al-chelator-interactions
#19
Gabriele Dalla Torre, Jon I Mujika, Elena Formoso, Eduard Matito, Maria J Ramos, Xabier Lopez
Due to aluminum's controversial role in neurotoxicity, the goal of chelation therapy, the removal of the toxic metal ion or attenuation of its toxicity by transforming it into less toxic compounds, has attracted considerable interest in the past years. In the present paper we present, validate and apply a state-of-the-art theoretical protocol suitable for the characterization of the interactions between a chelating agent and Al(iii). In particular, we employ a cluster-continuum approach based on Density Functional Theory calculations to evaluate the binding affinity of aluminum for a set of two important families of aromatic chelators: salicylic acids and catechols...
June 19, 2018: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/29916392/ultrastructural-and-molecular-characteristics-of-kudoa-crenimugilis-n-sp-infecting-intestinal-smooth-muscle-of-fringelip-mullet-crenimugil-crenilabis-in-the-red-sea
#20
Abdel-Azeem S Abdel-Baki, Heba M Abdel-Haleem, Saleh Al-Quraishy, Carlos Azevedo, Lamjed Mansour
This study describes infection of intestinal smooth muscle in fringelip mullets Crenimugil crenilabis with Kudoa crenimugilis n. sp. Of 30 individuals sampled from the Red Sea off Saudi Arabia, 6 (20%) were infected. Ovoid plasmodia (279-412 × 157-295 µm) in the smooth muscle of the intestine were packed with only mature myxospores with 4 valves. Specifically, light and transmission electron microscopy revealed quadrate myxospores with 4 equal, rounded, spore valves uniting at thin delicate suture lines. The mature myxospores were 8 (7-9) µm long, 5...
June 19, 2018: Diseases of Aquatic Organisms
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