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https://www.readbyqxmd.com/read/28445987/context-dependent-mir-204-and-mir-211-affect-the-biological-properties-of-amelanotic-and-melanotic-melanoma-cells
#1
Marianna Vitiello, Andrea Tuccoli, Romina D'Aurizio, Samanta Sarti, Laura Giannecchini, Simone Lubrano, Andrea Marranci, Monica Evangelista, Silvia Peppicelli, Chiara Ippolito, Ivana Barravecchia, Elena Guzzolino, Valentina Montagnani, Michael Gowen, Elisa Mercoledi, Alberto Mercatanti, Laura Comelli, Salvatore Gurrieri, Lawrence W Wu, Omotayo Ope, Keith Flaherty, Genevieve M Boland, Marc R Hammond, Lawrence Kwong, Mario Chiariello, Barbara Stecca, Gao Zhang, Alessandra Salvetti, Debora Angeloni, Letizia Pitto, Lido Calorini, Giovanna Chiorino, Marco Pellegrini, Meenhard Herlyn, Iman Osman, Laura Poliseno
Despite increasing amounts of experimental evidence depicting the involvement of non-coding RNAs in cancer, the study of BRAFV600E-regulated genes has thus far focused mainly on protein-coding ones. Here, we identify and study the microRNAs that BRAFV600E regulates through the ERK pathway.By performing small RNA sequencing on A375 melanoma cells and a vemurafenib-resistant clone that was taken as negative control, we discover miR-204 and miR-211 as the miRNAs most induced by vemurafenib. We also demonstrate that, although belonging to the same family, these two miRNAs have distinctive features...
April 11, 2017: Oncotarget
https://www.readbyqxmd.com/read/28445787/adipose-derived-stem-cells-were-impaired-in-restricting-cd4-t-cell-proliferation-and-polarization-in-type-2-diabetic-apoe-mouse
#2
Ming-Hao Liu, Ya Li, Lu Han, Yao-Yuan Zhang, Di Wang, Zhi-Hao Wang, Hui-Min Zhou, Ming Song, Yi-Hui Li, Meng-Xiong Tang, Wei Zhang, Ming Zhong
BACKGROUND: Atherosclerosis (AS) is the most common and serious complication of type 2 diabetes mellitus (T2DM) and is accelerated via chronic systemic inflammation rather than hyperglycemia. Adipose tissue is the major source of systemic inflammation in abnormal metabolic state. Pro-inflammatory CD4(+)T cells play pivotal role in promoting adipose inflammation. Adipose-derived stem cells (ADSCs) for fat regeneration have potent ability of immunosuppression and restricting CD4(+)T cells as well...
April 23, 2017: Molecular Immunology
https://www.readbyqxmd.com/read/28445618/ficolin-3-adiponectin-ratio-for-the-prediction-of-gestational-diabetes-mellitus-in-pregnant-women
#3
Xiao-Song Yuan, Hui Shi, Hui-Yan Wang, Bin Yu, Jian Jiang
AIMS/INTRODUCTION: To establish that the ficolin-3/adiponectin ratio is a predictor for gestational diabetes mellitus (GDM) and was eligible for screening tests for GDM. MATERIALS AND METHODS: A prospective cohort study of 86 pregnant women who developed GDM and 273 normal glucose tolerance (NGT) participants was performed. Maternal serum ficolin-3, adiponectin levels were investigated at 16-18 weeks gestation using ELISA. RESULTS: Compared to the NGT group, the GDM group showed significantly higher levels of ficolin-3 and the ratio of ficolin-3/adiponectin; and decreased levels of adiponectin between 16 to 18 weeks of gestation (P < 0...
April 26, 2017: Journal of Diabetes Investigation
https://www.readbyqxmd.com/read/28445535/microrna-125a-3p-is-regulated-by-myd88-in-legionella-pneumophila-infection-and-targets-ntan1
#4
Elisa Jentho, Malena Bodden, Christine Schulz, Anna-Lena Jung, Kerstin Seidel, Bernd Schmeck, Wilhelm Bertrams
BACKGROUND: Legionella pneumophila (L. pneumophila) is a causative agent of severe pneumonia. It is highly adapted to intracellular replication and manipulates host cell functions like vesicle trafficking and mRNA translation to its own advantage. However, it is still unknown to what extent microRNAs (miRNAs) are involved in the Legionella-host cell interaction. METHODS: WT and MyD88-/- murine bone marrow-derived macrophages (BMM) were infected with L. pneumophila, the transcriptome was analyzed by high throughput qPCR array (microRNAs) and conventional qPCR (mRNAs), and mRNA-miRNA interaction was validated by luciferase assays with 3´-UTR mutations and western blot...
2017: PloS One
https://www.readbyqxmd.com/read/28445445/cardioprotective-effects-of-malvidin-against-isoproterenol-induced-myocardial-infarction-in-rats-a-mechanistic-study
#5
Hui Wei, Hui Li, Shu-Ping Wan, Qiu-Tang Zeng, Long-Xian Cheng, Li-Li Jiang, Yu-Dong Peng
BACKGROUND Malvidin (alvidin-3-glucoside) is a polyphenol that belongs to the class of natural anthocyanin, which is abundantly found in red wines, colored fruits, and the skin of red grapes. Therefore, the current investigation was intended to evaluate the effect of malvidin against myocardial infarction induced by isoproterenol in the rats. MATERIAL AND METHODS The cardioprotective effects was assessed by determining the effect of malvidin on the activities of endogenous antioxidants - catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GSH) - and on the levels of lipid peroxidation and serum marker enzymes...
April 26, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/28445413/identification-of-highly-specific-scfvs-against-total-adiponectin-for-diagnostic-purposes
#6
Peter Wilton, Michael Steidel, Gabriele Krczal, Iris Hermanns, Andreas Pfützner, Alisa Konnerth, Kajohn Boonrod
Adiponectin is one of the most abundant adipokines secreted from adipose tissue. It acts as an endogenous insulin sensitizer and plasma concentrations are inversely correlated with obesity and metabolic syndrome. A decrease in plasma adiponectin levels normally indicates increased hormonal activity of the visceral lipid tissue, which is associated with decreased insulin sensitivity. It may therefore be considered a valuable biomarker for elucidating the underlying deteriorations resulting in type 2 diabetes and macrovascular disease...
April 26, 2017: Biology
https://www.readbyqxmd.com/read/28445010/detection-of-serum-antibodies-to-hepatitis-e-virus-based-on-hev-genotype-3-orf2-capsid-protein-expressed-in-nicotiana-benthamiana
#7
Milena Mazalovska, Nikola Varadinov, Tsvetoslav Koynarski, Ivan Minkov, Pavel Teoharov, George P Lomonossoff, Gergana Zahmanova
BACKGROUND: Hepatitis E virus (HEV) causes epidemics in developing countries and is primarily transmitted through the fecal-oral route. There have been recent reports on the zoonotic spread of the virus, and several animal species, primarily pigs, have been recognized as reservoirs of HEV. Because of its possible spread, there is an urgent need of a method for the cost-effective production of HEV proteins that can be used as diagnostic antigens for the serological detection of anti-HEV antibodies...
July 2017: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/28444950/optical-coherence-tomography-evaluation-of-patients-with-macula-off-retinal-detachment-after-different-postoperative-posturing-a-randomized-pilot-study
#8
Enrico Peiretti, Francesco Nasini, Elisa Buschini, Giulia Caminiti, Sarit Y Lesnik Oberstein, Alissa Willig, Heico M Bijl, Marco Mura
PURPOSE: To assess the presence of outer and inner retinal folds (RFs) and drop-out of the ellipsoid zone (EZ) occurring after surgical repair of macula-off rhegmatogenous retinal detachment (RRD) with different postoperative posture and preoperative use of adjuvant perfluorocarbon liquid (PFCO). METHODS: In this prospective study, 56 eyes of 56 consecutive patients affected by RRD were subjected to 23- or 25-gauge pars plana vitrectomy (PPV). The patients were randomized in four groups (14 prone 5 hr without PFCO, 14 supine 5 hr without PFCO, 14 prone 5 hr with PFCO and 14 supine 5 hr with PFCO) and followed up with spectral domain optical coherence tomography (SD-OCT)...
April 26, 2017: Acta Ophthalmologica
https://www.readbyqxmd.com/read/28444877/identification-and-measurement-of-staphylococcal-enterotoxin-m-sem-from-staphylococcus-aureus-isolate-associated-with-staphylococcal-food-poisoning
#9
Yanying Zhao, Anni Zhu, Junni Tang, Cheng Tang, Juan Chen
Staphylococcus aureus (S. aureus) produces a wide variety of staphylococcal enterotoxins (SEs, SEA to SEX), which are responsible for staphylococcal food poisoning. This study is aimed to establish a system to detect SEM protein in food matrixes. In the present study, sem gene was characterized in a S. aureus isolate H4 associated with food poisoning. The amino acid sequence of the deduced SEM protein was same as that of previously identified SEM from S. aureus 04-02981. Subsequently, mature SEM protein was expressed in E...
April 26, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28444853/experimental-type-2-diabetes-induction-reduces-serum-vaspin-but-not-serum-omentin-in-wistar-rats
#10
Cynthia Aparecida de Castro, Karina Ana da Silva, Marina Molina Buffo, Karina Nogueira Zambone Pinto, Fernanda de Oliveira Duarte, Keico Okino Nonaka, Fernanda de Freitas Aníbal, Ana Cláudia Garcia de Oliveira Duarte
Vaspin and omentin are adipose tissue adipokines that have often been related to obesity and its comorbidities. The aim of this study was to investigate the behaviour of serum omentin and vaspin in models of type 2 diabetes. To do this, Wistar rats (~200 g) were randomly divided into two groups: a non-diabetic group (n = 6) and a diabetic group fed on a high-fat diet (n = 6) and a low dose of streptozotocin (Sigma(®) ). All procedures were approved by the Brazilian Ethics Committee. Body weight (BW) and food intake were recorded daily...
April 25, 2017: International Journal of Experimental Pathology
https://www.readbyqxmd.com/read/28444848/biologically-meaningful-scents-a-framework-for-understanding-predator-prey-research-across-disciplines
#11
Michael H Parsons, Raimund Apfelbach, Peter B Banks, Elissa Z Cameron, Chris R Dickman, Anke S K Frank, Menna E Jones, Ian S McGregor, Stuart McLean, Dietland Müller-Schwarze, Elisa E Sparrow, Daniel T Blumstein
Fear of predation is a universal motivator. Because predators hunt using stealth and surprise, there is a widespread ability among prey to assess risk from chemical information - scents - in their environment. Consequently, scents often act as particularly strong modulators of memory and emotions. Recent advances in ecological research and analytical technology are leading to novel ways to use this chemical information to create effective attractants, repellents and anti-anxiolytic compounds for wildlife managers, conservation biologists and health practitioners...
April 26, 2017: Biological Reviews of the Cambridge Philosophical Society
https://www.readbyqxmd.com/read/28444727/advances-in-understanding-the-pathogenesis-of-familial-myeloproliferative-neoplasms
#12
REVIEW
Elisa Rumi, Mario Cazzola
Myeloproliferative neoplasms (MPNs) are generally acquired as a result of a somatic stem cell mutation leading to clonal expansion of myeloid precursors. In addition to sporadic cases, familial MPN occurs when one or several MPN affect different relatives of the same family. MPN driver mutations (JAK2, CALR, MPL) are somatically acquired also in familial cases, so a genetic predisposition to acquire one of the MPN driver mutations would be inherited, even though the causative germline mutations underlying familial MPN remain largely unknown...
April 25, 2017: British Journal of Haematology
https://www.readbyqxmd.com/read/28444660/effects-of-cycling-and-exergaming-on-neurotrophic-factors-in-elderly-type-2-diabetic-men-a-preliminary-investigation
#13
Christian Brinkmann, Leonie Schäfer, Magd Masoud, Joachim Latsch, Daniel Lay, Wilhelm Bloch, Klara Brixius
Patients with type 2 diabetes mellitus (T2DM) are at increased risk of developing neurodegenerative diseases. There is growing evidence that repeated exercise-induced transient increases in neurotrophic factors can augment neurogenesis and neuroplasticity. This pilot study compares the effects of 30-min submaximal cycling with those of exergaming (combining exercise and video gaming) at the same duration and same rating of perceived exertion (BORG RPE: 14-15) on serum neurotrophic factors in 8 elderly non-insulin-dependent T2DM patients (71±4 years) (2×2 crossover design)...
April 25, 2017: Experimental and Clinical Endocrinology & Diabetes
https://www.readbyqxmd.com/read/28444543/cost-analysis-of-incisional-hernia-repair-with-synthetic-mesh-and-biological-mesh-an-italian-study
#14
Sabrina Rampado, Andrea Geron, Giovanni Pirozzolo, Angelica Ganss, Elisa Pizzolato, Romeo Bardini
Repair of an incisional hernia (IH) generates costs on several levels and domains of society. The aim of this study was to make a complete cost analysis of incisional hernia repair (IHR) with synthetic and biological mesh and to compare it with financial reimbursement. Patients were grouped into three levels to determine the complexity of their care, and hence, the costs involved. Group 1 included patients without comorbidities, who underwent a "standard" incisional hernia repair (SIHR), with synthetic mesh...
April 25, 2017: Updates in Surgery
https://www.readbyqxmd.com/read/28444534/metabolic-factors-anthropometric-measures-diet-and-physical-activity-in-long-term-breast-cancer-survivors-change-from-diagnosis-and-comparison-to-non-breast-cancer-controls
#15
Ana Elisa Lohmann, Marguerite Ennis, Sara K Taylor, Pamela J Goodwin
PURPOSE: We studied metabolic factors, diabetes, and anthropometric measurements at diagnosis and long-term follow-up (LTFU), mean 12.5 years post-diagnosis, in breast cancer (BC) survivors, and compared their status at LTFU to that of age-matched women without BC. Diet and physical activity were also assessed. METHOD: 535 non-diabetic BC patients treated at three University of Toronto hospitals were followed prospectively; 285 surviving patients, without distant recurrence, participated in a LTFU study...
April 25, 2017: Breast Cancer Research and Treatment
https://www.readbyqxmd.com/read/28444306/immunoglobulin-based-investigation-of-spontaneous-resolution-of-chlamydia-trachomatis-infection
#16
Rakesh Bakshi, Kanupriya Gupta, Stephen J Jordan, LaDraka' T Brown, Christen G Press, Rachel J Gorwitz, John R Papp, Sandra G Morrison, Jeannette Y Lee, Richard P Morrison, William M Geisler
Chlamydia trachomatis (CT) elementary body (EB) ELISA was used to investigate serum anti-CT IgG1 (long-lived response) and IgG3 (short-lived response indicating more recent infection) from treatment (enrollment) and 6-month follow-up visits in 77 women previously classified as having spontaneous resolution of chlamydia. 71.4% of women were IgG1+IgG3+, consistent with more recent chlamydia resolution. 15.6% were IgG3- at both visits, suggesting absence of recent chlamydia. Using EB ELISA, we demonstrated about one in six women classified as having spontaneous resolution of chlamydia might have been exposed to CT but not infected...
April 21, 2017: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28444120/premenopausal-hysterectomy-and-risk-of-ovarian-cancer-in-african-american-women
#17
Lauren C Peres, Anthony J Alberg, Elisa V Bandera, Jill Barnholtz-Sloan, Melissa Bondy, Michele L Cote, Ellen Funkhouser, Patricia G Moorman, Edward S Peters, Ann G Schwartz, Paul D Terry, Sarah E Abbott, Fabian Camacho, Frances Wang, Joellen M Schildkraut
Although the inverse association between hysterectomy and epithelial ovarian cancer (EOC) was considered well established, investigators in recent studies including women diagnosed after 2000 have observed modest increases in risk. Most studies have been conducted in white women with little representation of African-American women. We examined the relationship between premenopausal hysterectomy and EOC in African-American women and explored whether hormone therapy (HT) modified this association in 614 cases and 743 controls enrolled in the African American Cancer Epidemiology Study (2010-2015)...
April 21, 2017: American Journal of Epidemiology
https://www.readbyqxmd.com/read/28444107/apelin-13-inhibits-lipoprotein-lipase-expression-via-the-apj-pkc%C3%AE-mir-361-5p-signaling-pathway-in-thp-1-macrophage-derived-foam-cells
#18
Xin Zhang, Qiong Ye, Duo Gong, Yuan Lv, Haipeng Cheng, Chong Huang, Lingyan Chen, Zhenwang Zhao, Liang Li, Xie Wei, Min Zhang, Xiaodan Xia, Xiaohua Yu, Xilong Zheng, Shuzhi Wang, Zongbao Wang, Chaoke Tang
Atherosclerotic lesions are characterized by the accumulation of abundant lipids and chronic inflammation. Previous researches have indicated that macrophage-derived lipoprotein lipase (LPL) promotes atherosclerosis progression by accelerating lipid accumulation and pro-inflammatory cytokine secretion. Although apelin-13 has been regarded as an atheroprotective factor, it remains unclear whether it can regulate the expression of LPL. The aim of this study was to explore the effects of apelin-13 on the expression of LPL and the underlying mechanism in THP-1 macrophage-derived foam cells...
April 21, 2017: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/28444073/antibacterial-activity-of-standard-and-n-doped-titanium-dioxide-coated-endotracheal-tubes-an-in-vitro-study
#19
Valentina Caratto, Lorenzo Ball, Elisa Sanguineti, Angelo Insorsi, Iacopo Firpo, Stefano Alberti, Maurizio Ferretti, Paolo Pelosi
Objective: The aim of this study was to assess the antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa of two nanoparticle endotracheal tube coatings with visible light-induced photocatalysis. Methods: Two types of titanium dioxide nanoparticles were tested: standard anatase (TiO2) and N-doped TiO2 (N-TiO2). Nanoparticles were placed on the internal surface of a segment of commercial endotracheal tubes, which were loaded on a cellulose acetate filter; control endotracheal tubes were left without a nanoparticle coating...
January 2017: Revista Brasileira de Terapia Intensiva
https://www.readbyqxmd.com/read/28444071/reducing-pressure-ulcers-in-patients-with-prolonged-acute-mechanical-ventilation-a-quasi-experimental-study
#20
Cecilia Inés Loudet, María Cecilia Marchena, María Roxana Maradeo, Silvia Laura Fernández, María Victoria Romero, Graciela Esther Valenzuela, Isabel Eustaquia Herrera, Martha Teresa Ramírez, Silvia Rojas Palomino, Mariana Virginia Teberobsky, Leandro Ismael Tumino, Ana Laura González, Rosa Reina, Elisa Estenssoro
Objective: To determine the effectiveness of a quality management program in reducing the incidence and severity of pressure ulcers in critical care patients. Methods: This was a quasi-experimental, before-and-after study that was conducted in a medical-surgical intensive care unit. Consecutive patients who had received mechanical ventilation for ≥ 96 hours were included. A "Process Improvement" team designed a multifaceted interventional process that consisted of an educational session, a pressure ulcer checklist, a smartphone application for lesion monitoring and decision-making, and a "family prevention bundle"...
January 2017: Revista Brasileira de Terapia Intensiva
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