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https://www.readbyqxmd.com/read/29046026/-influence-of-lifestyle-diet-and-vitamin-d-on-atopy-in-a-population-of-afro-descendant-colombian-children
#1
Luis Carlos Fang-Mercado, Juan Ricardo Urrego-Álvarez, Andrés Ernesto Merlano-Barón, Catherine Meza-Torres, Luz Hernández-Bonfante, Liliana López-Kleine, Javier Marrugo-Cano
BACKGROUND: Allergic conditions have shown an increase in the past few decades. Hygiene, changes in lifestyle, diet and vitamin D have been blamed for this increase. OBJECTIVE: To determine the role of environment, diet and vitamin D in atopic diseases in a population of Colombian Afro-descendant children. METHODS: Cross-sectional, descriptive, observational study in 200 Afro-descendant children of rural and urban areas from northern Colombia...
July 2017: Revista Alergia Mexico: Organo Oficial de la Sociedad Mexicana de Alergia e Inmunología, A.C
https://www.readbyqxmd.com/read/29045972/-acetylsalicylic-acid-treatment-enhanced-immunomodulatory-function-of-mesenchymal-stem-cells-derived-from-gingiva
#2
R L Yang, T T Yu, Y H Zhou
OBJECTIVE: To analyze the role of acetylsalicylic acid (ASA) in immunomodulation of mesenchymal stem cells derived from gingiva (GMSCs), and to explore the role of ASA in enhancing the immumomodulation of GMSCs and the capacity of GMSCs to treat immune disorders and the underlying mechanism. METHODS: Flow cytometry analysis were used to analyze the role of ASA in the expression of stem cells surface markers CD146, CD105, CD90, CD34 and CD45 in GMSCs,and the GMSCs proliferation was analyzed by 5-bromo-2-deoxyuridine (BrdU) staining and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay...
October 18, 2017: Beijing da Xue Xue Bao. Yi Xue Ban, Journal of Peking University. Health Sciences
https://www.readbyqxmd.com/read/29045964/-increased-serum-c-c-chemokine-ligand-19-levels-correlated-with-b-cell-abnormalities-in-systemic-lupus-erythematosus
#3
H J Liu, L J Shi, F L Hu, H H Yao, Z G Li, Y Jia
OBJECTIVE: To detect the levels of serum C-C chemokine ligand 19 (CCL19) in patients with systemic lupus erythematosus (SLE) and to evaluate the correlation between CCL19 expression and clinical features and laboratory parameters, trying to reveal the possible role of CCL19 in the pathogenesis of systemic lupus erythematosus. METHODS: The levels of serum CCL19 were measured by enzyme linked immunosorbent assay (ELISA) in 90 patients with SLE and 30 healthy controls...
October 18, 2017: Beijing da Xue Xue Bao. Yi Xue Ban, Journal of Peking University. Health Sciences
https://www.readbyqxmd.com/read/29045850/comparison-of-conventional-2d-ultrasound-to-mr-imaging-for-prenatal-estimation-of-birth-weight-in-twin-pregnancy
#4
Caroline Kadji, Elisa Bevilacqua, Ivan Hurtado, Andrew Carlin, Mieke M Cannie, Jacques C Jani
BACKGROUND: During prenatal follow-up of twin pregnancies, accurate identification of birthweight and birthweight discordance is important in order to identify the high risk group and plan perinatal care. Unfortunately prenatal evaluation of birthweight discordance by 2-dimensional ultrasound (US) has been far from optimal. OBJECTIVE: To prospectively compare estimates of fetal weight based on 2-dimensional US (US-EFW) and MR imaging (MR-EFW) to actual birth weight in women carrying twin pregnancies...
October 15, 2017: American Journal of Obstetrics and Gynecology
https://www.readbyqxmd.com/read/29045840/clonal-heterogeneity-influences-the-fate-of-new-adaptive-mutations
#5
Ignacio Vázquez-García, Francisco Salinas, Jing Li, Andrej Fischer, Benjamin Barré, Johan Hallin, Anders Bergström, Elisa Alonso-Perez, Jonas Warringer, Ville Mustonen, Gianni Liti
The joint contribution of pre-existing and de novo genetic variation to clonal adaptation is poorly understood but essential to designing successful antimicrobial or cancer therapies. To address this, we evolve genetically diverse populations of budding yeast, S. cerevisiae, consisting of diploid cells with unique haplotype combinations. We study the asexual evolution of these populations under selective inhibition with chemotherapeutic drugs by time-resolved whole-genome sequencing and phenotyping. All populations undergo clonal expansions driven by de novo mutations but remain genetically and phenotypically diverse...
October 17, 2017: Cell Reports
https://www.readbyqxmd.com/read/29045788/the-importance-of-surface-irox-in-stabilizing-ruo2-for-oxygen-evolution
#6
Maria Escudero-Escribano, Anders F Pedersen, Elisa A Paoli, Rasmus Frydendal, Daniel Friebel, Paolo Malacrida, Jan Rossmeisl, Ifan E L Stephens, Ib Chorkendorff
The high precious metal loading and high overpotential of the oxygen evolution reaction (OER) prevents the widespread utilization of polymer electrolyte membrane (PEM) water electrolyzers. Herein we explore the OER activity and stability in acidic electrolyte of a combined IrOx/RuO2 system consisting of RuO2 thin films with sub-monolayer (1, 2 and 4 Å) amounts of IrOx deposited on top. Operando extended X-ray absorption fine structure (EXAFS) on the Ir L-3 edge revealed a rutile type IrO2 structure with some Ir sites occupied by Ru, IrOx being at the surface of the RuO2 thin film...
October 18, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/29045565/cigarette-sidestream-smoke-delays-nucleotide-excision-repair-inhibited-accumulation-of-repair-proteins-at-dna-lesions
#7
Guang Yang, Yuko Ibuki
Cigarette sidestream smoke (CSS) contains many carcinogens that induce DNA damage. DNA damage plays an important role in the initiation of cancer and several diseases, and repair is the major defense mechanism; however, the relationship between CSS and the repair of DNA damage remains unclear. We herein investigated whether CSS influences nucleotide excision repair (NER) in vivo and in vitro. HR-1 hairless mouse skin treated with CSS was exposed to UVB, as a result of which pyrimidine dimers (cyclobutane pyrimidine dimers (CPDs) and pyrimidine(6-4)pyrimidone photoproducts (6-4PPs)) were formed and repaired via the NER pathway...
October 13, 2017: Carcinogenesis
https://www.readbyqxmd.com/read/29045486/thiamine-deficiency-activates-hypoxia-inducible-factor-1%C3%AE-to-facilitate-pro-apoptotic-responses-in-mouse-primary-astrocytes
#8
Kristy Zera, Jason Zastre
Thiamine is an essential enzyme cofactor required for proper metabolic function and maintenance of metabolism and energy production in the brain. In developed countries, thiamine deficiency (TD) is most often manifested following chronic alcohol consumption leading to impaired mitochondrial function, oxidative stress, inflammation and excitotoxicity. These biochemical lesions result in apoptotic cell death in both neurons and astrocytes. Comparable histological injuries in patients with hypoxia/ischemia and TD have been described in the thalamus and mammillary bodies, suggesting a congruency between the cellular responses to these stresses...
2017: PloS One
https://www.readbyqxmd.com/read/29045037/autoantibodies-targeting-ficolin-2-in-systemic-lupus-erythematosus-patients-with-active-nephritis
#9
Sophie Colliard, Noémie Jourde-Chiche, Giovanna Clavarino, Françoise Sarrot-Reynauld, Evelyne Gout, Alban Deroux, Mélanie Fougere, Nathalie Bardin, Laurence Bouillet, Jean-Yves Cesbron, Nicole M Thielens, Chantal Dumestre-Pérard
OBJECTIVE: Systemic lupus erythematosus (SLE) is a multi-system inflammatory disease characterized by production of various autoantibodies. The aim of this study was to investigate the presence of anti-ficolin-2 antibodies in SLE patients and to evaluate the association between the levels of these autoantibodies, clinical manifestations, and disease activity. METHODS: This is a comparative study using a cohort of 165 SLE patients and 48 healthy subjects. SLE patients were further divided into two groups, with "low disease activity" (SLEDAI score ≤ 4, n = 88) and with "high disease activity" (SLEDAI score > 4, n = 77)...
October 17, 2017: Arthritis Care & Research
https://www.readbyqxmd.com/read/29044636/an-inverse-association-between-serum-resistin-levels-and-n-3-polyunsaturated-fatty-acids-intake-was-strongest-in-the-snp-420-g-g-genotype-in-the-japanese-cohort-the-toon-genome-study
#10
Yukinobu Noumi, Ryoichi Kawamura, Yasuharu Tabara, Koutatsu Maruyama, Yasunori Takata, Wataru Nishida, Ai Okamoto, Tatsuya Nishimiya, Hiroshi Onuma, Isao Saito, Takeshi Tanigawa, Haruhiko Osawa
OBJECTIVE: Resistin is secreted from monocytes/macrophages and is associated with insulin resistance, inflammation, and cardiovascular diseases. In the Japanese cohort, serum resistin is tightly associated with a single nucleotide polymorphism (SNP) at -420 (rs1862513) in the promoter region of the human resistin gene. However, interactions between SNP-420 and environmental factors remain to be elucidated. The aim of this study was to investigate the association between serum resistin levels and nutrient intake, and the effect of SNP-420 on this association...
October 17, 2017: Clinical Endocrinology
https://www.readbyqxmd.com/read/29044469/co-differentiation-of-human-pluripotent-stem-cells-derived-cardiomyocytes-and-endothelial-cells-from-cardiac-mesoderm-provides-a-three-dimensional-model-of-cardiac-microtissue
#11
Elisa Giacomelli, Milena Bellin, Valeria V Orlova, Christine L Mummery
The formation of cardiac mesodermal subtypes is highly regulated in time and space during heart development. In vitro models based on human pluripotent stem cells (hPS cells) provide opportunities to study mechanisms underlying fate choices governing lineage specification from common cardiovascular progenitors in human embryos. The generation of cardiac endothelial cells in particular allows the creation of complex models of cardiovascular disorders in which either cardiomyocytes or endothelial cells are affected...
October 18, 2017: Current Protocols in Human Genetics
https://www.readbyqxmd.com/read/29044421/heterogeneous-hcg-and-hmg-commercial-preparations-result-in-different-intracellular-signalling-but-induce-a-similar-long-term-progesterone-response-in-vitro
#12
Laura Riccetti, Danièle Klett, Mohammed Akli Ayoub, Thomas Boulo, Elisa Pignatti, Simonetta Tagliavini, Manuela Varani, Tommaso Trenti, Alessia Nicoli, Francesco Capodanno, Giovanni Battista La Sala, Eric Reiter, Manuela Simoni, Livio Casarini
STUDY QUESTION: Are four urinary hCG/menotropin (hMG) and one recombinant preparation characterized by different molecular features and do they mediate specific intracellular signaling and steroidogenesis? SUMMARY ANSWER: hCG and hMG preparations have heterogeneous compositions and mediate preparation-specific cell signaling and early steroidogenesis, although similar progesterone plateau levels are achieved in 24 h-treated human primary granulosa cells in vitro...
October 1, 2017: Molecular Human Reproduction
https://www.readbyqxmd.com/read/29044041/viral-haemorrhagic-septicaemia-virus-vhsv-id-infections-are-detected-more-consistently-using-syndromic-vs-active-surveillance
#13
Pia Vennerström, Elina Välimäki, Tapani Lyytikäinen, Maria Hautaniemi, Gabriele Vidgren, Perttu Koski, Anna-Maija Virtala
The eradication of viral haemorrhagic septicaemia virus (VHSV Id) from Finnish brackish-water rainbow trout Oncorhynchus mykiss farms located in the restriction zone in the Province of Åland, Baltic Sea, failed several times in the 2000s. The official surveillance programme was often unable to find VHSV-positive populations, leading to the misbelief in the fish farming industry that virus eradication could be achieved. The ability of 3 other surveillance programmes to detect infected fish populations was compared with the official programme...
October 18, 2017: Diseases of Aquatic Organisms
https://www.readbyqxmd.com/read/29043993/serum-levels-of-leptin-zinc-and-tryptophan-in-obese-subjects-with-sleep-deficits
#14
Noreen Samad, Farzana Yasmin, Suad Naheed, Abu Zeeshan Bari, M Mazhar Ayaz, Aqal Zaman, Atif Akbar, Saima Saeed, Najeeb Ullah
Obesity is an important risk factor for sleep disorders. This study aimed to evaluate the association of leptin, zinc and tryptophan (TRP) in obese subjects with sleep deficits [sleep apnea (SA), insomnia (IN)]. In this cross sectional case control, with the verbal and written consent 206, obese with sleep deficits and 30, non-obese/normal identified from various areas of Karachi, Pakistan. The socio-demographic data including; age, body mass index (BMI), education and residence, of participants was collected...
July 2017: Pakistan Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/29043661/development-of-adenosine-deaminase-specific-igy-antibodies-diagnostic-and-inhibitory-application
#15
Agnieszka Łupicka-Słowik, Mateusz Psurski, Renata Grzywa, Kamila Bobrek, Patrycja Smok, Maciej Walczak, Andrzej Gaweł, Tadeusz Stefaniak, Józef Oleksyszyn, Marcin Sieńczyk
Adenosine deaminase (ADA) is currently used as a diagnostic marker for tuberculous pleuritis. Although ADA has been suggested as a potential marker for several types of cancer, the importance of each of ADA isoforms as well as their levels and enzymatic activities in tumors need to be further investigated. Herein we developed avian immunoglobulin Y highly specific to human ADA via hens immunization with calf adenosine deaminase. The obtained antibodies were used for the development of a sensitive double-egg yolk immunoglobulin (IgY) sandwich ELISA assay with an ADA detection limit of 0...
October 17, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/29042994/immunological-mechanism-of-low-dose-priming-radiation-resistance-in-walker-256-tumor-model-mice
#16
Li Feng, Ling Qin, Dan Guo, Daping Deng, Feng Lu, Hailiang Li, Narisu Bao, Xiting Yang, Hongyu Ding, Jianguo Li
The aim of the present study was to investigate whether low-dose priming radiation induces antitumor immunity that can be augmented by the modulation of natural killer (NK) cell and cytokine activity using a mouse tumor model. Walker-256 cells were injected into the right flank of male BALB/c mice. At 7 days after inoculation, mice were divided into three groups, including group 1,2,3. In group 1 the mice were without radiation, in group 2 the mice were received 2 Gy radiation only, and in group 3 the mice were radiated with a priming dose of 75 mGy followed by 2 Gy radiation after 24 h...
October 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29042992/timosaponin-b-iii-exhibits-antidepressive-activity-in-a-mouse-model-of-postpartum-depression-by-the-regulation-of-inflammatory-cytokines-bndf-signaling-and-synaptic-plasticity
#17
Xiao-Li Zhang, Lin Wang, Li Xiong, Feng-Hua Huang, Han Xue
The aim of this study was to investigate the antidepressive effects of timosaponin B-III (TB-III) and the underlying mechanism. A postpartum depression (PPD) mouse model was established by the administration of dexamethasone sodium phosphate during pregnancy. Mice with PPD were assigned to the following groups: Model, fluoxetine and high, medium and low doses of TB-III. Post-parturient mice without PPD served as a normal control group. To examine the effect of TB-III, mice were treated with TB-III, then forced swimming tests (FSTs) and tail suspension tests (TSTs) were performed to evaluate depression...
October 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29042990/changes-of-treg-and-th17-cells-as-well-as-cytokines-in-children-with-acute-bronchitis
#18
Zhiqiang Tu, Haiyan Xue, Wei Chen, Lanfang Cao, Weiqi Zhang
The present study aimed to investigate changes of T-regulatory (Treg) and T-helper (Th)17 cells as well as cytokines in peripheral blood of children with acute bronchitis, and to explore the roles of these cells in the pathogenesis of acute bronchitis. A total of 126 children who had presented at Renji Hospital (Shanghai, China) with acute bronchitis were selected as the observation group and 30 healthy children were selected as the control group. Th17/Tregs in the peripheral blood of the children of the observation group and the control group was detected by flow cytometry...
October 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29042973/the-efficacy-of-microsurgery-in-the-treatment-of-cerebral-aneurysm-rupture-and-its-effect-on-nf-%C3%AE%C2%BAb-mcp-1-and-mmp-9
#19
Xintong Zhang, Lei Chen, Feng Zheng, Yanli Du
The clinical efficacy of microsurgical neck clipping for the treatment of cerebral aneurysm rupture and its effect on serum nuclear factor κ-light-chain-enhancer of activated β cells (NF-κB), monocyte chemoattractant protein-1 (MCP-1) and matrix metalloproteinase-9 (MMP-9) levels were investigated. A total of 56 patients with first occurrence of cerebral aneurysm rupture were enrolled from June 2015 to June 2016. These patients were divided into control (25 patients) and observation groups (31 patients) according to treatment received...
October 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29042954/dexmedetomidine-ameliorates-the-inflammatory-immune-response-in-rats-with-acute-kidney-damage
#20
Weihua Liu, Wenli Yu, Yiqi Weng, Yongwang Wang, Mingwei Sheng
It has been demonstrated that dexmedetomidine (Dex) can protect patients with acute kidney injury from experiencing further tissue damage, however its mechanism of action remains unclear. The present study investigated the immune modulatory functions of Dex in rats with acute kidney injury (AKI) induced via injection of lipopolysaccharide into the tail vein. ELISA analysis showed that Dex reduced the levels of inflammatory cytokines in rats with AKI in a dose dependent manner. Furthermore, the regulatory effects of Dex on cytokine production disappeared when the α-2 adrenergic receptor antagonist Yohimbine (YOH) was added...
October 2017: Experimental and Therapeutic Medicine
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