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https://www.readbyqxmd.com/read/29668134/-anti-trypanosoma-cruzi-antibodies-in-latin-american-migrants-in-transit-through-the-m%C3%A3-xico-usa-border
#1
Laura Mayela Montes-Rincón, Lucio Galaviz-Silva, Zinnia Judith Molina-Garza
INTRODUCTION: In recent years, American trypanosomiasis has become an emergent public health problem in countries receiving migrant populations such as México, USA, Canada or those in Europe. OBJECTIVE: To analyze the prevalence of anti-Trypanosoma cruzi antibodies in Latin American migrants on their way to USA and Canada by means of serological techniques. MATERIAL AND METHODS: ELISA and IHA were performed to detect anti-T. cruzi antibodies...
March 15, 2018: Biomédica: Revista del Instituto Nacional de Salud
https://www.readbyqxmd.com/read/29668132/-shtlv-i-ii-seroprevalence-in-blood-donors-of-hospital-pablo-tob%C3%A3-n-uribe-blood-bank-during-the-period-2014-2015
#2
Manuela Muñoz, Santiago Carvalho, Jorge Hernando Donado, Gloria Eugenia Barco, Sergio Jaramillo
INTRODUCTION: The human-T cell lymphotropic virus is a retrovirus with various types known so far. HTLV-I and HTLV-II are of clinically importance as they cause different diseases such as adult T-cell leukemia/lymphoma, tropical spastic paraparesis, and human T-lymphotropic virus type I-associated myelopathy (HAM). OBJECTIVE: To estimate the prevalence of presumptive and confirmatory reactivity to HTLV-I/II in blood donors of Hospital Pablo Tobón Uribe Blood Bank between 2014 and 2015...
March 15, 2018: Biomédica: Revista del Instituto Nacional de Salud
https://www.readbyqxmd.com/read/29668078/adherence-and-immune-response-to-revaccination-following-hematopoietic-stem-cell-transplantation-at-a-pediatric-onco-hematology-reference-center
#3
Fernanda Gouveia-Alves, Roseane Gouveia, Valéria C Ginani, Adriana Seber, Danielle A Kuramoto, Gabriel F A Murad, Fernanda G Spina, Antonio S Petrilli, Victor G Zecchin, Celso Granato, Fabianne Carlesse, M Isabel de Moraes-Pinto
BACKGROUND: Revaccination after hematopoietic stem cell transplantation (HSCT) is necessary to compensate for the loss of immunological memory. The aims of this study were to evaluate the adherence to revaccination schedule and the humoral immune response to different vaccine antigens in HSCT pediatric and young adult patients. METHODS: Patients submitted to HSCT for over 3 years were recruited. After written informed consent, a questionnaire was filled in, the vaccination card was analyzed, a blood sample was collected and tested by ELISA for diphtheria, Haemophilus influenzae type b (Hib), hepatitis A, hepatitis B, tetanus, measles, rubella and varicella antibodies...
April 18, 2018: Transplant Infectious Disease: An Official Journal of the Transplantation Society
https://www.readbyqxmd.com/read/29667907/direct-peritoneal-resuscitation-reduces-leukocyte-infiltration-in-the-kidney-after-acute-brain-death
#4
Jessica Lee Weaver, Paul J Matheson, Amy Matheson, Victoria S Graham, Cynthia Downard, Richard Neal Garrison, Jason W Smith
Brain death is associated with significant inflammation within the kidneys, which may contribute to reduced graft survival. Direct peritoneal resuscitation (DPR) has been shown to reduce systemic inflammation after brain death. To determine its effects, brain dead rats were resuscitated with normal saline (targeted intravenous fluid, TIVF) to maintain a mean arterial pressure of 80 mmHg and DPR animals also received 30cc of intraperitoneal peritoneal dialysis solution. Rats were euthanized at zero, two, four, and six hours after brain death...
April 18, 2018: American Journal of Physiology. Renal Physiology
https://www.readbyqxmd.com/read/29667546/seminal-supar-levels-as-marker-of-abacterial-male-accessory-gland-inflammation-in-hypogonadism
#5
Domenico Milardi, Giuseppe Grande, Chiara Autilio, Francesca Mancini, Laura De Marinis, Riccardo Marana, Cecilia Zuppi, Andrea Urbani, Alfredo Pontecorvi, Silvia Baroni
Recent evidences suggest that hypogonadism is an important risk factor for lower urinary tract symptoms and benign prostatic hyperplasia. Several papers have discussed the role of chronic inflammation in the development of BPH, which may be modulated by the hypogonadal state. Soluble urokinase-type plasminogen activator receptor (suPAR), known protein marker of systemic inflammation, can be assayed in the seminal plasma and represents a reliable and sensitive marker of inflammation for the male accessory gland inflammation (MAGI)...
April 18, 2018: Protein and Peptide Letters
https://www.readbyqxmd.com/read/29667111/propofol-specifically-suppresses-il-1%C3%AE-secretion-but-increases-bacterial-survival-in-staphylococcus-aureus-infected-raw264-7-cells
#6
Ming-Shan Chen, Wen-Chun Lin, Hsuan-Te Yeh, Chia-Lin Hu, Shew-Meei Sheu
Anesthetics have immunomodulatory effects, but the use of different assay systems has contributed to inconsistent results in the literature. IL-1β and reactive oxygen species (ROS) secreted by phagocytes are important factors that protect against Staphylococcus aureus infection. In this study, the effects of four intravenous anesthetics (propofol, thiamylal sodium, midazolam, and ketamine) on IL-1β secretion, ROS, and bacterial survival in S. aureus-infected RAW264.7 cells were evaluated. S. aureus-infected RAW264...
April 17, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/29666624/recombinant-protein-containing-b-cell-epitopes-of-different-loxosceles-spider-toxins-generates-neutralizing-antibodies-in-immunized-rabbits
#7
Sabrina de Almeida Lima, Clara Guerra-Duarte, Fernanda Costal-Oliveira, Thais Melo Mendes, Luís F M Figueiredo, Daysiane Oliveira, Ricardo A Machado de Avila, Valéria Pereira Ferrer, Dilza Trevisan-Silva, Silvio S Veiga, João C Minozzo, Evanguedes Kalapothakis, Carlos Chávez-Olórtegui
Loxoscelism is the most important form of araneism in South America. The treatment of these accidents uses heterologous antivenoms obtained from immunization of production animals with crude loxoscelic venom. Due to the scarcity of this immunogen, new alternatives for its substitution in antivenom production are of medical interest. In the present work, three linear epitopes for Loxosceles astacin-like protease 1 (LALP-1) (SLGRGCTDFGTILHE, ENNTRTIGPFDYDSIMLYGAY, and KLYKCPPVNPYPGGIRPYVNV) and two for hyaluronidase (LiHYAL) (NGGIPQLGDLKAHLEKSAVDI and ILDKSATGLRIIDWEAWR) from Loxosceles intermedia spider venom were identified by SPOT-synthesis technique...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29666623/autoimmunity-to-tropomyosin-specific-peptides-induced-by-mycobacterium-leprae-in-leprosy-patients-identification-of-mimicking-proteins
#8
Itu Singh, Asha Ram Yadav, Keshar Kunja Mohanty, Kiran Katoch, Prashant Sharma, Vinay Kumar Pathak, Deepa Bisht, Umesh D Gupta, Utpal Sengupta
Background: It has been shown earlier that there is a rise in the levels of autoantibodies and T cell response to cytoskeletal proteins in leprosy. Our group recently demonstrated a rise in both T and B cell responses to keratin and myelin basic protein in all types of leprosy patients and their associations in type 1 reaction (T1R) group of leprosy. Objectives: In this study, we investigated the association of levels of autoantibodies and lymphoproliferation against myosin in leprosy patients across the spectrum and tried to find out the mimicking proteins or epitopes between host protein and protein/s of Mycobacterium leprae ...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29666038/correlation-of-serum-ccl3-mip-1%C3%AE-levels-with-disease-severity-in-postmenopausal-osteoporotic-females
#9
Hui Wan, Tie-Yong Qian, Xiao-Jing Hu, Ci-You Huang, Wei-Feng Yao
BACKGROUND: The pro-inflammatory protein chemokine cytokine ligand 3 is well-established as a vital regulator of bone resorption and osteoclasts stimulation. AIMS: We sought to investigate if serum chemokine cytokine ligand 3 levels correlated with disease severity in postmenopausal osteoporosis females. STUDY DESIGN: Cross-sectional Study. METHODS: 82 postmenopausal osteoporotic females, 76 postmenopausal non-osteoporotic women and 80 healthy female at childbearing age were recruited...
April 15, 2018: Balkan Medical Journal
https://www.readbyqxmd.com/read/29665925/-expression-of-hmgb1-in-spleen-of-adult-patients-with-chronic-and-refractory-immune-thrombocytopenia-and-its-significance
#10
Peng-Feng Yang, Guo-Yang Zhang, Hong-Yun Liu, Shuang-Feng Xie, Xiu-Ju Wang, Xiao-Yan Liu, Jue Wang, Xin-Yuan Chen, Shuo Yang, Li-Ping Ma
OBJECTIVE: To investigate the expression and clinical significance of high mobility group box 1(HMGB1) in spleen of adult patients with chronic and refractory immune thrombocytopenia(ITP). METHODS: Twenty chronic and refactory ITP patients received splenectomy were enrolled in ITP group and 20 cases of traumatic spleen rupture were enrolled in control group. The splenectomy efficacy in ITP patients was analyzed retrospectively. The HMGB1 expression in spleen tissue was detected by immunohistochemistry, and the correlation between different expression levels of HMGB1 and splenectomy efficacy were analysed...
April 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://www.readbyqxmd.com/read/29665910/-effect-of-coagulation-and-platelet-activation-on-tumor-associated-hypercoagulable-state-in-lymphoma-bearing-mice
#11
Miao Zhu, Qing-Qing Shi, Xin Sun, Jun Ni, Wei Wu, Lian-Jun Shen, Wei Ji, Kai-Lin Xu, Jian Gu
OBJECTIVE: To investigate the coagulation abnormality and tumor-associated hypercoagulable state in lymphoma-bearing mice by measuring the changes in coagulation indices (D-D, vWF, TF) and platelet activation indices (P-selectin, GPIIbIIIa). METHODS: The mouse model with lymphoma was established by the subcutaneous injection of 38B9 lymphoma cells into BALB/c mice, and the tumor formation was evaluated by using MRI and B ultrasonography. The D-D, vWF and TP levels of blood samples from inner canthal vein of tumor-bearing mice on 1 d, 14 d and 21 d were detected by using ELISA, the platelet activation indices (P-selectin, GPIIbIIIa) were detected by using flow cytometry...
April 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://www.readbyqxmd.com/read/29665864/recurrent-pregnancy-loss-is-associated-to-leaky-gut-a-novel-pathogenic-model-of-endometrium-inflammation
#12
C Tersigni, S D'Ippolito, F Di Nicuolo, R Marana, V Valenza, V Masciullo, F Scaldaferri, F Malatacca, C de Waure, A Gasbarrini, G Scambia, N Di Simone
BACKGROUND: Recurrent pregnancy loss (RPL) occurs in 3-5% in about 30% of cases no cause can be found. Women with RPL show higher prevalence of undiagnosed gut disorders. Furthermore, in endometrial tissues of RPL women, higher expression of pro-inflammatory cytokines and Nalp-3 inflammasome has been observed. Aim of this study was to investigate whether an abnormal gut permeability might occur in RPL women and allow passage into systemic circulation of pro-inflammatory molecules able to induce endometrial inflammation...
April 17, 2018: Journal of Translational Medicine
https://www.readbyqxmd.com/read/29665846/correlation-of-serum-levels-of-fibroblast-growth-factor-23-and-klotho-protein-levels-with-bone-mineral-density-in-maintenance-hemodialysis-patients
#13
Shubei Zheng, Yan Chen, Yu Zheng, Zhihong Zhou, Zhanyuan Li
OBJECTIVE: The correlation of serum fibroblast growth factor 23 (FGF-23) and Klotho protein levels with bone mineral density (BMD) in maintenance hemodialysis (MHD) patients was analyzed. METHODS: Between January 2015 and November 2015, 125 MHD patients in our hospital were enrolled. Dual-energy X-ray absorptiometry was used to examine the BMD in the femoral neck and lumbar spine of MHD patients. The patients were divided into three groups: a normal bone mass group, an osteopenia group, and an osteoporosis group...
April 17, 2018: European Journal of Medical Research
https://www.readbyqxmd.com/read/29665817/elevated-il-17-levels-in-semi-immune-anaemic-mice-infected-with-plasmodium-berghei-anka
#14
Gideon Kofi Helegbe, Nguyen Tien Huy, Tetsuo Yanagi, Mohammed Nasir Shuaibu, Mihoko Kikuchi, Mahamoud Sama Cherif, Kenji Hirayama
BACKGROUND: Alterations in inflammatory cytokines and genetic background of the host contribute to the outcome of malaria infection. Despite the promising protective role of IL-17 in infections, little attention is given to further understand its importance in the pathogenesis of severe malaria anaemia in chronic/endemic situations. The objective of this study, therefore, was to evaluate IL-17 levels in anaemic condition and its association with host genetic factors. METHODS: Two mice strains (Balb/c and CBA) were crossed to get the F1 progeny, and were (F1, Balb/c, CBA) taken through 6 cycles of Plasmodium berghei (ANKA strain) infection and chloroquine/pyrimethamine treatment to generate semi-immune status...
April 17, 2018: Malaria Journal
https://www.readbyqxmd.com/read/29665814/herd-level-seroprevalence-of-fasciola-hepatica-and-ostertagia-ostertagi-infection-in-dairy-cattle-population-in-the-central-and-northeastern-poland
#15
Sławomir J Kowalczyk, Michał Czopowicz, Corinna N Weber, Elisabeth Müller, Tomasz Nalbert, Andrzej Bereznowski, Jarosław Kaba
BACKGROUND: Fasciola hepatica and Ostertagia ostertagi infections are widespread in cattle population of Europe, however data on their prevalence in Poland are only fragmentary. Therefore, the cross-sectional study was carried out to determine the herd-level seroprevalence of F. hepatica and O. ostertagi infection in dairy cattle population in the central and north-eastern provinces Poland, and to identify basic local risk factors for these infections. In total, 598 herds were enrolled, 394 (65...
April 17, 2018: BMC Veterinary Research
https://www.readbyqxmd.com/read/29665770/dietary-diversity-score-is-associated-with-cardiovascular-risk-factors-and-serum-adiponectin-concentrations-in-patients-with-metabolic-syndrome
#16
Mahdieh Abbasalizad Farhangi, Leila Jahangiry
BACKGROUND: Metabolic syndrome is associated with cardio-metabolic risk factors and lipid abnormalities. Previous studies evaluated the dietary habits and nutrient intakes among patients with metabolic syndrome; however the association between metabolic risk factors and adiponectin with dietary diversity score (DDS) in patients with metabolic syndrome has not been evaluated yet. Therefore the aim of the current study was to evaluate these relationships among patients with metabolic syndrome...
April 17, 2018: BMC Cardiovascular Disorders
https://www.readbyqxmd.com/read/29665669/-effects-of-intermittent-hypoxia-exposure-on-renal-tubular-epithelial-cell-injury-and-autophagy-in-rats
#17
C Wang, J Tan, B Y Chen, Q Zhang
Objective: To investigate the effects of intermittent hypoxia (IH) exposure on renal tubular epithelial cell injury and autophagy in rats. Methods: A total of 12 Sprague Dawley (SD) rats were randomly divided into two groups: normal control (NC) group and IH group, with 6 rats in each group. An IH animal model was established to observe the effects of IH on renal tubular epithelial cell damage and autophagy in rats. Microalbumin (mAlb) was detected by immunoturbidimetry. Neutrophil gelatinase-associated lipocalin (NGAL) and kidney injury molecule-1(KIM-1)in urine were assessed by enzyme-linked immunosorbent assay (ELISA) test...
March 27, 2018: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/29665643/evaluation-of-the-effects-of-iranian-propolis-on-the-severity-of-post-operational-induced-peritoneal-adhesion-in-rats
#18
Vahid Reza Askari, Vafa Baradaran Rahimi, Parvin Zamani, Narges Fereydouni, Pouria Rahmanian-Devin, Amir Hossein Sahebkar, Hassan Rakhshandeh
BACKGROUND: Peritoneal adhesion is a major complication of surgery that can lead to serious problems such as bowel obstruction, pain, infertility and even mortality. Propolis is a honey bee product with anti-inflammatory and anti-oxidant activities that could potentially protect against adhesive surgical complications. METHODS: Forty 8-weeks-old rats (275 ± 25 g) were divided into five groups: normal group without any surgical procedure, and experimental groups treated with normal saline, 50 mg/kg, 100 mg/kg and 200 mg/kg of propolis...
March 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29665497/the-effects-of-resiquimod-in-an-ovalbumin-induced-allergic-rhinitis-model
#19
Shenhong Qu, Taijie Qin, Min Li, Shaojie Zhang, Linsong Ye, Jiazhang Wei, Hua Fan, Baiwen Chen
Growing evidence indicates that the Toll-like receptor7/8(TLR7/8) agonist resiquimod (R848) is a potential inhibitor of type-2 immunity. However, the mechanisms mediating its therapeutic effects are not fully understood. This study investigated the effects of R848 on OVA-induced allergic rhinitis(AR) mice and the expression of IL-25, IL-33, TSLP, T-cell immunoglobulin mucin1 (TIM1) and T-cell immunoglobulin mucin3 (TIM3). BALB/c mice were intranasally sensitized and challenged with ovalbumin (OVA), and R848 was intraperitoneally injected into AR mice...
April 14, 2018: International Immunopharmacology
https://www.readbyqxmd.com/read/29665437/multiplex-serology-of-helicobacter-pylori-antigens-in-detection-of-current-infection-and-atrophic-gastritis-a-simple-and-cost-efficient-method
#20
Ebrahim Shafaie, Samaneh Saberi, Maryam Esmaeili, Zeynab Karimi, Saeed Najafi, Mohammad Tashakoripoor, Afshin Abdirad, Mahmoud Eshagh Hosseini, Mohammad Ali Mohagheghi, Vahid Khalaj, Marjan Mohammadi
INTRODUCTION: Helicobacter pylori express a large array of antigens, each of which is duly responsible for successful colonization and pathogenesis. Here, we have studied host serum antibody responses to four of its immunodominant antigens in association with the infection status and the resulting clinical outcomes. METHODS: For this purpose, four individual H. pylori proteins (UreB, CagA, Tip-α and HP0175) were produced in recombinant forms. Serum antibody responses of 246 (75 GC and 171 NUD) patients, against the above antigens, were evaluated by multiplex immunoblotting...
April 14, 2018: Microbial Pathogenesis
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