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https://www.readbyqxmd.com/read/28551846/ampk-and-sirt1-activation-contribute-to-inhibition-of-neuroinflammation-by-thymoquinone-in-bv2-microglia
#1
Ravikanth Velagapudi, Abdelmeneim El-Bakoush, Izabela Lepiarz, Folashade Ogunrinade, Olumayokun A Olajide
Thymoquinone is a known inhibitor of neuroinflammation. However, the mechanism(s) involved in its action remain largely unknown. In this study, we investigated the roles of cellular reactive oxygen species (ROS), 5' AMP-activated protein kinase (AMPK) and sirtuin 1 (SIRT1) in the anti-neuroinflammatory activity of thymoquinone. We investigated effects of the compound on ROS generation in LPS-activated microglia using the fluorescent 2',7'-dichlorofluorescin diacetate (DCFDA)-cellular ROS detection. Immunoblotting was used to detect protein levels of p40(phox), gp91(phox), AMPK, LKB1 and SIRT1...
May 27, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28551822/glucocorticoid-therapy-causes-contradictory-changes-of-serum-wnt-signaling-related-molecules-in-systemic-autoimmune-diseases
#2
Mai Kawazoe, Kaichi Kaneko, Kotaro Shikano, Natsuko Kusunoki, Toshihiro Nanki, Shinichi Kawai
The objective of this study was to investigate the clinical significance of the Wnt/β-catenin signaling pathway in glucocorticoid-induced osteoporosis. A total of 91 patients with systemic autoimmune diseases who received initial glucocorticoid therapy with prednisolone (30-60 mg daily) were prospectively enrolled. We measured serum levels of N-terminal peptide of type I procollagen (P1NP), bone alkaline phosphatase (BAP), tartrate-resistant acid phosphatase isoform 5b (TRACP-5b), N-telopeptide cross-linked type I collagen (NTX), sclerostin, Dickkopf-1 (Dkk-1), and Wnt3a before starting glucocorticoid therapy and every week for 4 weeks after its initiation...
May 27, 2017: Clinical Rheumatology
https://www.readbyqxmd.com/read/28551756/extracerebral-dysfunction-in-animal-models-of-autism-spectrum-disorder
#3
Elisa L Hill-Yardin, Sonja J McKeown, Gaia Novarino, Andreas M Grabrucker
Genetic factors might be largely responsible for the development of autism spectrum disorder (ASD) that alone or in combination with specific environmental risk factors trigger the pathology. Multiple mutations identified in ASD patients that impair synaptic function in the central nervous system are well studied in animal models. How these mutations might interact with other risk factors is not fully understood though. Additionally, how systems outside of the brain are altered in the context of ASD is an emerging area of research...
2017: Advances in Anatomy, Embryology, and Cell Biology
https://www.readbyqxmd.com/read/28551653/clinical-relevance-of-serum-her2-and-circulating-tumor-cell-detection-in-metastatic-breast-cancer-patients
#4
Malgorzata Banys-Paluchowski, Isabell Witzel, Sabine Riethdorf, Brigitte Rack, Wolfgang Janni, Peter Andreas Fasching, Erich-Franz Solomayer, Bahriye Aktas, Sabine Kasimir-Bauer, Klaus Pantel, Tanja Fehm, Volkmar Müller
BACKGROUND/AIM: Presence of circulating tumor cells (CTCs) is associated with impaired survival in metastatic breast cancer (MBC). This study was designed to evaluate whether assessment of serum HER2 (sHER2) levels provide additional prognostic information in MBC. MATERIALS AND METHODS: Two hundred and fifty-three MBC patients were enrolled in this multicentre trial. CTCs were detected before the start of first- or later-line treatment using the CellSearch system...
June 2017: Anticancer Research
https://www.readbyqxmd.com/read/28551548/quantitative-evaluation-of-atherosclerotic-plaques-and-intraplaque-neovascularization-using-contrast-enhanced-ultrasound-after-treatment-with-atorvastatin-in-rabbits
#5
Hong-Yu Liu, Jia Zhou, Hai Tong, Yan Tang, Xiao-Fang Wang, Qi-Chang Zhou
INTRODUCTION: This study is supposed to investigate the value of contrast-enhanced ultrasound (CEUS) in quantitative evaluation of atherosclerotic plaques and intraplaque neovascularization after treatment with atorvastatin (ATV) in rabbits. MATERIAL AND METHODS: Forty-five New Zealand white rabbits were enrolled to construct the rabbit model of AS. All rabbits were assigned into the control, AS group and ATV groups (n=15 individually). The AS plaque formation and relative parameters were observed and calculated by CEUS respectively...
May 25, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28551532/effects-of-tryptophan-kynurenine-and-kynurenic-acid-exerted-on-human-reconstructed-corneal-epithelium-in-vitro
#6
Anna Matysik-Woźniak, Roman Paduch, Waldemar A Turski, Ryszard Maciejewski, Anselm G Jünemann, Robert Rejdak
BACKGROUND: Tryptophan metabolites formed along kynurenine pathway may affect cell proliferation and tissue function. This pathway presents potential sites for drug discovery. Tryptophan and its metabolites kynurenine and kynurenic acid may be involved in the physiology and pathology of the ocular surface. The purpose of this work is to investigate the effect exerted by tryptophan, kynurenine and kynurenic acid upon corneal epithelium. METHODS: A SkinEthic™ HCE human reconstructed corneal epithelium model was used...
February 24, 2017: Pharmacological Reports: PR
https://www.readbyqxmd.com/read/28551507/serum-chemerin-in-children-with-excess-body-weight-may-be-associated-with-ongoing-metabolic-complications-a-pilot-study
#7
Marta Sledzińska, Agnieszka Szlagatys-Sidorkiewicz, Michał Brzezinski, Katarzyna Kaźmierska, Tomasz Sledziński, Barbara Kamińska
PURPOSE: The aim of this study was to verify if serum chemerin in children correlates with body weight, fat mass, selected inflammatory markers, parameters of liver function, lipid and glucose metabolism. MATERIALS AND METHODS: The study included children aged 5-17 years with normal body weight (<85th BMI percentile, n=43) or overweight (≥85th BMI percentile, n=58). Serum concentrations of chemerin were determined with ELISA. RESULTS: Children with excess body weight presented with significantly higher serum concentrations of chemerin...
May 25, 2017: Advances in Medical Sciences
https://www.readbyqxmd.com/read/28551451/a-sandwich-elisa-for-the-detection-of-neuraminidase-of-avian-influenza-a-h7n9-virus
#8
Yueyang Yu, Xi Zhang, Baihui Zhao, Ying Sun, Xiaoguan Zhang, Tian Bai, Jian Lu, Zi Li, Liqi Liu, Dayan Wang, Yuelong Shu, Jianfang Zhou, Kun Qin
Although exhibiting no or low virulence in poultry, avian influenza virus H7N9 has caused around 1,200 confirmed human infections in China with a case-fatality rate of 30% since 2013. A highly pathogenic H7N9 virus (HP-H7), with the HA antigenicity distinct from the previous, were recently detected in patients and poultry. Therefore, convenient rapid diagnosis with reliability will allow early antiviral use and management for H7N9 infection. Here, a sandwich ELISA targeting the conserved viral antigen, neuraminidase (NA) was developed...
May 24, 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/28551395/a-sandwich-elisa-for-porcine-alpha-1-acid-glycoprotein-pagp-orm-1-and-further-demonstration-of-its-use-to-evaluate-growth-potential-in-newborn-pigs
#9
T J Caperna, A E Shannon, M Stoll, S Kahl, L A Blomberg, J L Vallet, T G Ramsay
A simple, reproducible sandwich, ELISA was developed to measure porcine alpha-1 acid glycoprotein (pAGP, ORM-1) in pig plasma. Porcine AGP isolated from serum was purchased and a polyclonal antisera was prepared in rabbits using the whole pAGP molecule as immunogen. The antiserum was affinity purified, and a portion of the purified antibody fraction was labeled with horseradish peroxidase. Porcine AGP protein was used as a standard, whereas commercially available buffers and reagents were utilized throughout the assay...
April 8, 2017: Domestic Animal Endocrinology
https://www.readbyqxmd.com/read/28551371/an-evaluation-of-purified-salmonella-typhi-protein-antigens-for-the-serological-diagnosis-of-acute-typhoid-fever
#10
Nga Tran Vu Thieu, Tan Trinh Van, Anh Tran Tuan, Elizabeth J Klemm, Chau Nguyen Ngoc Minh, Phat Voong Vinh, Duy Pham Thanh, Thanh Ho Ngoc Dan, Trung Pham Duc, Pinky Langat, Laura B Martin, Jorge Galan, Li Liang, Philip L Felgner, D Huw Davies, Hanna K de Jong, Rapeephan R Maude, Masako Fukushima, Lalith Wijedoru, Aniruddha Ghose, Rasheda Samad, Arjen M Dondorp, Abul Faiz, Thomas C Darton, Andrew J Pollard, Guy E Thwaites, Gordon Dougan, Christopher M Parry, Stephen Baker
OBJECTIVES: The diagnosis of typhoid fever is a challenge. Aiming to develop a typhoid diagnostic we measured antibody responses against Salmonella Typhi (S. Typhi) protein antigens and the Vi polysaccharide in a cohort of Bangladeshi febrile patients. METHODS: IgM against 12 purified antigens and the Vi polysaccharide was measured by ELISA in plasma from patients with confirmed typhoid fever (n=32), other confirmed infections (n=17), and healthy controls (n=40)...
May 24, 2017: Journal of Infection
https://www.readbyqxmd.com/read/28551357/padi4-polymorphisms-and-the-functional-haplotype-are-associated-with-increased-rheumatoid-arthritis-susceptibility-a-replication-study-in-a-southern-mexican-population
#11
Christian Johana Baños-Hernández, José Eduardo Navarro Zarza, Isela Parra Rojas, Mirna Vázquez Villamar, Jorge Ramón Padilla-Gutiérrez, Yeminia Valle, Zyanya Reyes-Castillo, Nora Magdalena Torres-Carrillo, Samuel García-Arellano, Lorena Michele Brennan-Bourdon, José Francisco Muñoz-Valle
Rheumatoid arthritis (RA) is a common autoimmune disease with a complex genetic background. The peptidyl arginine deiminase type IV (PADI4) gene has been associated with RA susceptibility in several populations. We addressed the relationship between three exonic PADI4 gene single nucleotide polymorphisms (SNPs) PADI4_89 (rs11203366), PADI4_90 (rs11203367) and PADI4_92 (rs874881) and related haplotypes with RA in a population from Southern México. This study included 200 RA patients and 200 control subjects...
May 24, 2017: Human Immunology
https://www.readbyqxmd.com/read/28551308/indirect-elisa-ielisa-for-routine-detection-of-antibodies-against-minute-virus-of-mice-mvm-in-mice-colonies
#12
Juan M Laborde, Guillermo H Sguazza, Nadia A Fuentealba, Santiago G Corva, Cecilia Carbone, Cecilia M Galosi
In this study we developed an indirect ELISA to detect antibodies against Minute Virus of Mice (MVM) using an antigen produced from BHK-21 cells infected with a prototype strain of the virus. The optimal antigen concentration and serum dilutions were established. In order to analyze variability in the laboratory, reproducibility and repeatability within and between plates were determined. Then, a panel of 460 sera from conventional facilities and previously classified as positive or negative by the indirect fluorescent antibody assay was analyzed...
May 24, 2017: Revista Argentina de Microbiología
https://www.readbyqxmd.com/read/28551218/the-feasibility-of-harmonizing-gluten-elisa-measurements
#13
Malgorzata Rzychon, Marcel Brohée, Fernando Cordeiro, Reka Haraszi, Franz Ulberth, Gavin O'Connor
Many publications have highlighted that routine ELISA methods do not give rise to equivalent gluten content measurement results. In this study, we assess this variation between results and its likely impact on the enforcement of the EU gluten-free legislation. This study systematically examines the feasibility of harmonizing gluten ELISA assays by the introduction of: a common extraction procedure; a common calibrator, such as a pure gluten extract and an incurred matrix material. The comparability of measurements is limited by a weak correlation between kit results caused by differences in the selectivity of the methods...
November 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28551129/quantitative-determination-of-vegf165-in-cell-culture-medium-by-aptamer-sandwich-based-chemiluminescence-assay
#14
Siwen Shan, Ziyi He, Sifeng Mao, Mingsha Jie, Linglu Yi, Jin-Ming Lin
In this work, we have developed a sensitive and selective chemiluminescence (CL) assay for vascular endothelial growth factor (VEGF165) quantitative detection based on two specific VEGF165 binding aptamers (Apt). VEGF is a predominant biomarker in cancer angiogenesis, and sensitive detection method of VEGF are highly demanded for both academic study and clinical diagnosis of multiple cancers. In our experiment, VEGF165 was captured in a sandwich structure assembled by two binding aptamers, one capture aptamer was immobilized on streptavidin-coated magnetic beads (MBs) and another VEGF-binding aptamer was labeled by biotin for further phosphatase conjunction...
August 15, 2017: Talanta
https://www.readbyqxmd.com/read/28550834/-bullous-pemphigoid-successfully-treated-with-rituximab
#15
Nuno Silva, Andreia Costa, Fernando Salvador, Elisa Serradeiro
Bullous pemphigoid is an autoimmune disease characterized by subepidermal blister formation. Can be self-limiting but when necessary treatment includes the use of corticosteroids, immunosuppressants, and biological agents. We present the case of a 37 year old patient with a severe and refractory presentation of bullous pemphigoid. After about a month of treatment with various first line drugs and with no response, we chose to start rituximab and clear improvement of the lesions was observed. The authors aim to highlight the usefulness and effectiveness of this drug in this disease...
March 31, 2017: Acta Médica Portuguesa
https://www.readbyqxmd.com/read/28550752/intracellular-delivery-of-oligonucleotides-in-helicobacter-pylori-by-fusogenic-liposomes-in-the-presence-of-gastric-mucus
#16
Rita S Santos, George R Dakwar, Elisa Zagato, Toon Brans, Céu Figueiredo, Koen Raemdonck, Nuno F Azevedo, Stefaan C De Smedt, Kevin Braeckmans
The rising antimicrobial resistance contributes to 25000 annual deaths in Europe. This threat to the public health can only be tackled if novel antimicrobials are developed, combined with a more precise use of the currently available antibiotics through the implementation of fast, specific, diagnostic methods. Nucleic acid mimics (NAMs) that are able to hybridize intracellular bacterial RNA have the potential to become such a new class of antimicrobials and additionally could serve as specific detection probes...
May 20, 2017: Biomaterials
https://www.readbyqxmd.com/read/28550594/comparison-of-rapid-diagnostic-tests-to-detect-mycobacterium-avium-subsp-paratuberculosis-disseminated-infection-in-bovine-liver
#17
Mehdi Zarei, Masoud Ghorbanpour, Samaneh Tajbakhsh, Nader Mosavari
Mycobacterium avium subsp. paratuberculosis (MAP) causes Johne's disease, a chronic enteritis in cattle and other domestic and wild ruminants. The presence of MAP in tissues other than intestines and associated lymph nodes, such as meat and liver, is a potential public health concern. In the present study, the relationship between the results of rapid diagnostic tests of the Johne's disease, such as serum ELISA, rectal scraping PCR, and acid-fast staining, and the presence of MAP in liver was evaluated. Blood, liver, and rectal scraping samples were collected from 200 slaughtered cattle with unknown Johne's disease status...
May 27, 2017: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/28550400/association-study-of-cd154-polymorphisms-and-serum-cd154-level-with-systemic-lupus-erythematous-in-chinese-population
#18
Yang Xiang, Jing Guo, You-Fan Peng, Hua-Tuo Huang, Yan Lan, Ye-Sheng Wei
The aim of this study was to investigate the association of three polymorphisms of CD154 with risk of SLE in Chinese population. The study population comprised 770 Chinese individuals, including 350 SLE patients and 420 healthy controls. The gene polymorphism was measured using Snapshot SNP genotyping assays and confirmed by sequencing. Serum CD154 (sCD154) level was measured by ELISA. Compared with control group, sCD154 levels were significantly increased in case group (P < 0.001). The minor C allele of rs1126535 was associated with a significantly increased risk of SLE as compared to the major T allele (P < 0...
May 26, 2017: Rheumatology International
https://www.readbyqxmd.com/read/28550395/a-critical-role-for-il-21-receptor-signaling-in-the-coxsackievirus-b3-induced-myocarditis
#19
Fan Yang, Xiao-Mou Wei, Wen-Wu Liang, Wen-Hong Mo, Bao-Ping Tan, Hong Wang
To determine whether IL-21 receptor signaling plays a significant role in promoting Tfh cell-mediated cardiac injury in viral myocarditis (VMC), we compared IL-21R-deficient mice for some parameters of VMC. Balb/c and IL-21R(-/-) mice were infected with CVB3. Frequencies of splenic Tfh cells were determined by flow cytometric analysis, and productions of anti-adenine nucleotide translocator (ANT) autoantibodies were detected by enzyme-linked immunosorbent assay. To determine the effects of IL-21R signal on the proliferation of B cells, lymphocytes from spleens of the IL-21R(-/-) and Balb/c mice infected by CVB3 were tagged with carboxyfluorescein succinimidyl ester (CFSE) and then were stimulated with lipopolysaccharides plus IL-21 or anti-IL-21 neutralizing antibody for 3 days...
May 26, 2017: Inflammation
https://www.readbyqxmd.com/read/28550388/antibodies-against-linear-epitopes-on-goodpasture-autoantigen-in-patients-with-anti-neutrophil-cytoplasmic-antibody-associated-vasculitis
#20
Xiao-Yu Jia, Jun-Tao Yu, Shui-Yi Hu, Jian-Nan Li, Miao Wang, Chen Wang, Min Chen, Zhao Cui, Ming-Hui Zhao
In a substantial number of patients with crescentic glomerulonephritis, both anti-glomerular basement membrane (GBM) antibodies and anti-neutrophil cytoplasmic antibodies (ANCA) are detected simultaneously. ANCA is presumed to be the initial event but the mechanism is unknown. In the present study, we investigated the antibodies against linear epitopes on Goodpasture autoantigen in sera from patients with ANCA-associated vasculitis, aiming to reveal the mechanisms of the coexistence of the two kinds of autoantibodies...
May 26, 2017: Clinical Rheumatology
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