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https://www.readbyqxmd.com/read/29150331/anti-amyloidogenic-and-anti-apoptotic-effect-of-%C3%AE-bisabolol-against-a%C3%AE-induced-neurotoxicity-in-pc12-cells
#1
Balakrishnan Shanmuganathan, Venkatesan Suryanarayanan, Sethuraman Sathya, Muralidharan Narenkumar, Sanjeev Kumar Singh, Kandasamy Ruckmani, Kasi Pandima Devi
Alzheimer's disease (AD) is a life-threatening neurodegenerative disorder leading to dementia, with a progressive decline in memory and other thinking skills of elderly populace. Of the multiple etiological factors of AD, the accumulation of senile plaques (SPs) particularly as Aβ oligomers correlates with the relentlessness cognitive impairment in AD patients and play a vital role in AD pathology. Since natural essential oil constituents have successfully served as a source of drugs for AD treatment, the present study aims at the in vitro and in silico investigation of anti-amyloidogenic potential and anti-apoptotic property of the α-bisabolol against Aβ25-35 induced neurotoxicity in PC12 cells...
October 10, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/29148269/salidroside-attenuates-neuroinflammation-and-improves-functional-recovery-after-spinal-cord-injury-through-microglia-polarization-regulation
#2
Chenggui Wang, Qingqing Wang, Yiting Lou, Jianxiang Xu, Zhenhua Feng, Yu Chen, Qian Tang, Gang Zheng, Zengjie Zhang, Yaosen Wu, Naifeng Tian, Yifei Zhou, Huazi Xu, Xiaolei Zhang
Spinal cord injury (SCI) is a severe neurological disease; however, few drugs have been proved to treat SCI effectively. Neuroinflammation is the major pathogenesis of SCI secondary injury and considered to be the therapeutic target of SCI. Salidroside (Sal) has been reported to exert anti-inflammatory effects in airway, adipose and myocardial tissue; however, the role of Sal in SCI therapeutics has not been clarified. In this study, we showed that Sal could improve the functional recovery of spinal cord in rats as revealed by increased BBB locomotor rating scale, angle of incline, and decreased cavity of spinal cord injury and apoptosis of neurons in vivo...
November 17, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/29142171/sub-toxic-concentrations-of-nano-zno-and-nano-tio2-suppress-neurite-outgrowth-in-differentiated-pc12-cells
#3
Tomohiko Irie, Tsuyoshi Kawakami, Kaoru Sato, Makoto Usami
Nanomaterials have been extensively used in our daily life, and may also induce health effects and toxicity. Nanomaterials can translocate from the outside to internal organs, including the brain. For example, both nano-ZnO and nano-TiO2 translocate into the brain via the olfactory pathway in rodents, possibly leading to toxic effects on the brain. Although the effects of nano-ZnO and nano-TiO2 on neuronal viability or neuronal excitability have been studied, no work has focused on how these nanomaterials affect neuronal differentiation and development...
2017: Journal of Toxicological Sciences
https://www.readbyqxmd.com/read/29133430/the-dual-function-of-the-polybasic-juxtamembrane-region-of-syntaxin-1a-in-clamping-spontaneous-release-and-stimulating-ca-2-triggered-release-in-neuroendocrine-cells
#4
Dafna Singer-Lahat, Noa Barak-Broner, Anton Sheinin, Dafna Greitzer-Antes, Izhak Michaelevski, Ilana Lotan
The exact function of the polybasic juxtamembrane region (5RK) of the plasma membrane neuronal SNARE, syntaxin 1A (Syx), in vesicle exocytosis, although widely studied, is currently not clear. Here, we addressed the role of 5RK in Ca(2+)-triggered release, using our Syx-based intramolecular FRET probe, which previously allowed us to resolve a depolarization-induced Ca(+2)- dependent close-to-open transition (CDO) of Syx that occurs concomitant with evoked release, both in PC12 cells and hippocampal neurons and was abolished upon charge neutralization of 5RK...
November 13, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/29130945/maternally-expressed-gene-3-meg3-enhances-pc12-cell-hypoxia-injury-by-targeting-mir-147
#5
Lili Han, Zhiling Dong, Ningning Liu, Fei Xie, Ning Wang
BACKGROUND/AIMS: Cerebral ischemia often leads to breakdown of blood-brain barrier (BBB) and vasogenic edema. It remains to be established whether MEG3 is responsible for the hypoxic damage in neural cells. This study aimed to investigate the role of MEG3 in the hypoxia-induced injuries of PC12 cells. METHODS: The PC12 cells were seeded and cultured under hypoxia and normoxia culture conditions. The cell viability determined by trypan blue exclusion, apoptosis using propidium iodide (PI) and fluorescein isothiocynate (FITC)-conjugated Annexin V staining, cell-migration using a modified two-chamber migration assay with a pore size of 8 µM and invasion using 24-well Millicell Hanging Cell Culture inserts with 8 µM PET membranes...
October 31, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29130486/rtp801-is-a-critical-factor-in-the-neurodegeneration-process-of-a53t-alpha-synuclein-in-a-mouse-model-of-parkinson-s-disease-under-chronic-restraint-stress
#6
Zhao Zhang, Shi-Feng Chu, Sha-Sha Wang, Yi-Na Jiang, Yan Gao, Peng-Fei Yang, Qi-Di Ai, Nai-Hong Chen
BACKGROUND AND PURPOSE: The incidence of Parkinson's disease exhibited a younger tendency in recent years with the constantly increased stressors of modern society, but this relationship remains obscured. We performed this study to investigate whether stress contributes to this tendency and identify the executor during this process. EXPERIMENTAL APPROACH: Ten-month-old α-synuclein A53T mice, a PD mice model, were treated with chronic restraint stress (CRS) to simulate a PD-sensitive person with constant stress stimulation...
November 11, 2017: British Journal of Pharmacology
https://www.readbyqxmd.com/read/29128369/inhibition-of-poly-adp-ribose-polymerase-1-alters-expression-of-mitochondria-related-genes-in-pc12-cells-relevance-to-mitochondrial-homeostasis-in-neurodegenerative-disorders
#7
Grzegorz A Czapski, Magdalena Cieślik, Przemysław L Wencel, Sylwia Wójtowicz, Robert P Strosznajder, Joanna B Strosznajder
Alzheimer's disease (AD) is characterized by the release of amyloid beta peptides (Aβ) in the form of monomers/oligomers which may lead to oxidative stress, mitochondria dysfunction, synaptic loss, neuroinflammation and, in consequence, to overactivation of poly(ADP-ribose) polymerase-1 (PARP-1). However, Aβ peptides are also released in the brain ischemia, traumatic injury and in inflammatory response. PARP-1 is suggested to be a promising target in therapy of neurodegenerative disorders. We investigated the impact of PARP-1 inhibition on transcription of mitochondria-related genes in PC12 cells...
November 8, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29126723/design-synthesis-and-evaluation-of-ndga-analogues-as-potential-anti-ischemic-stroke-agents
#8
Lili Huang, Jiabing Wang, Liping Chen, Min Zhu, Shoubiao Wu, Shenghui Chu, Yuantie Zheng, Ziliang Fan, Jiafeng Zhang, Wulan Li, Dahui Chen, Xiufei Yang, Sicen Wang, Peihong Qiu, Jianzhang Wu
Exogenous supplementation of antioxidants with ROS scavenging activity would be a potential therapy to cerebral ischemia-reperfusion injury in stroke. In the present study, a series of NDGA analogues with attenuation oxidative stress by directly scavenging ROS and indirectly through keap1/Nrf2/ARE pathway activation were designed and synthesized. All analogues were found to effectively remove ROS directly by DPPH radical scavenging assay, and compound 3a conferred potent protection from the oxidative injury in PC12 cells via promoting Nrf2 to translocate into nucleus and increasing expression of heme oxygenase-1(HO-1), where strongly reduced intracellular ROS level indirectly...
October 16, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/29124300/xanthoceraside-modulates-nr2b-containing-nmda-receptors-at-synapses-and-rescues-learning-memory-deficits-in-app-ps1-transgenic-mice
#9
Lin Zhu, Lei Yang, Xuemei Zhao, Danyang Liu, Xiaoli Guo, Peng Liu, Tianyan Chi, Xuefei Ji, Libo Zou
RATIONALE: Alzheimer's disease (AD) is characterized by memory loss and synaptic damage. Previous studies suggested that xanthoceraside decreases glutamate-induced PC12 cell death, ameliorates memory deficits, and increases the number of dendritic spines in AD mice. These results indicated that xanthoceraside might have activities that protect synaptic plasticity. Herein, we detected the effect of xanthoceraside on synaptic function. MATERIALS AND METHODS: Three-month-old APP/PS1 transgenic mice were orally treated with xanthoceraside (0...
November 9, 2017: Psychopharmacology
https://www.readbyqxmd.com/read/29123977/sodium-hydrosulfide-attenuates-cerebral-ischemia-reperfusion-injury-by-suppressing-overactivated-autophagy-in-rats
#10
Wen-Wu Jiang, Bai-Sheng Huang, Yang Han, Lv-Hong Deng, Li-Xiang Wu
Ischemic stroke is a leading cause of death and disability worldwide, and autophagy may be involved in the pathological process of cerebral ischemia/reperfusion injury. Hydrogen sulfide (H2S) is an endogenous gasotransmitter with protective effects against multiple diseases. Here, we tested the effect of H2S on cerebral ischemia/reperfusion injury in rats. Sodium hydrosulfide (NaHS), an H2S donor, improved neurological function and reduced the size of the infarcts induced by transient middle cerebral artery occlusion (MCAO) followed by reperfusion in rats...
November 2017: FEBS Open Bio
https://www.readbyqxmd.com/read/29120941/effects-of-gynosaponin-tn-2-on-l-dopa-induced-cytotoxicity-in-pc12-cells
#11
Keon Sung Shin, Hyun Jin Park, Keun Hong Park, Kyung Sook Lee, Sung Woo Jeong, Bang Yeon Hwang, Chong Kil Lee, Myung Koo Lee
Gynosaponins have pharmacological effects on 3,4-L-dihydroxyphenylalanine (L-DOPA)-related or dopamine-related neurological diseases; however, the neuroprotective functions of single compound of gynosaponins remain undefined. This study investigated the cytotoxic effects of gynosaponin TN-2 on L-DOPA in pheochromocytoma 12 cells. Gynosaponin TN-2, at 0.5-3 μM, did not exhibit cytotoxicity and protected against L-DOPA (100 and 200 μM)-induced cell death. Gynosaponin TN-2 (0.5 and 1.0 μM) inhibited the L-DOPA (100 and 200 μM)-induced sustained extracellular signal-regulated protein kinases 1 and 2 phosphorylation...
November 8, 2017: Neuroreport
https://www.readbyqxmd.com/read/29119451/pristimerin-inhibits-lps-triggered-neurotoxicity-in-bv-2-microglia-cells-through-modulating-irak1-traf6-tak1-mediated-nf-%C3%AE%C2%BAb-and-ap-1-signaling-pathways-in-vitro
#12
Bin Hui, Liping Zhang, Qinhua Zhou, Ling Hui
Microglia plays a prominent role in the brain's inflammatory response to injury or infection by migrating to affected locations and secreting inflammatory molecules. However, hyperactivated microglial is neurotoxic and plays critical roles in the pathogenesis of neurodegenerative diseases. Pristimerin, a naturally occurring triterpenoid, possesses antitumor, antioxidant, and anti-inflammatory activities. However, the effect and the molecular mechanism of pristimerin against lipopolysaccharide (LPS)-induced neurotoxicity in microglia remain to be revealed...
November 8, 2017: Neurotoxicity Research
https://www.readbyqxmd.com/read/29115511/neuroprotective-effect-of-polysaccharides-from-gastrodia-elata-blume-against-corticosterone%C3%A2-induced-apoptosis-in-pc12-cells-via-inhibition-of-the-endoplasmic-reticulum-stress%C3%A2-mediated-pathway
#13
Benhong Zhou, Jun Tan, Chan Zhang, Yue Wu
Depression is a common mental health disorder and is the leading cause of disability worldwide. Gastrodia elata (G. elata) was demonstrated to exhibit a neuroprotective effect in the authors' previous study. The present study investigated the effect of polysaccharides from G. elata (GEP) on PC12 cell apoptosis induced by corticosterone (CORT) and its possible underlying mechanisms. PC12 cells were treated with 200 µM CORT in the absence or presence of different concentrations of GEP for 48 h. Then, cell viability was measured by CCK‑8 assay...
November 3, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29115393/neuroprotective-effects-of-hydrogen-sulfide-on-sodium-azide-induced-oxidative-stress-in-pc12-cells
#14
Cheng Gao, Pan Chang, Lijun Yang, Yi Wang, Shaohua Zhu, Haiyan Shan, Mingyang Zhang, Luyang Tao
Alzheimer's disease (AD) is the most common neurodegenerative disorder, responsible for >50% of all dementia cases. Sodium azide (NaN3) inhibits cytochrome oxidase by irreversibly binding to the heme cofactor and selectively reducing the complex IV activity, which is present in post‑mortem AD brains. Previous data demonstrated that hydrogen sulfide (H2S), the third endogenous gaseous mediator, exerted protective effects against neuronal damage. Therefore, it was hypothesized that H2S may be able to scavenge excess reactive oxygen species (ROS), thereby protecting against oxidative stress and cell death...
November 1, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29115371/a-novel-method-of-neural-differentiation-of-pc12-cells-by-using-opti-mem-as-a-basic-induction-medium
#15
Rendong Hu, Qiaoyu Cao, Zhongqing Sun, Jinying Chen, Qing Zheng, Fei Xiao
The PC12 cell line is a classical neuronal cell model due to its ability to acquire the sympathetic neurons features when deal with nerve growth factor (NGF). In the present study, the authors used a variety of different methods to induce PC12 cells, such as Opti-MEM medium containing different concentrations of fetal bovine serum (FBS) and horse serum compared with RPMI-1640 medium, and then observed the neurite length, differentiation, adhesion, cell proliferation and action potential, as well as the protein levels of axonal growth-associated protein 43 (GAP-43) and synaptic protein synapsin-1, among other differences...
October 19, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29113024/neuroprotective-effects-of-butanol-fraction-of-cordyceps-cicadae-on-glutamate-induced-damage-in-pc12-cells-involving-oxidative-toxicity
#16
Dan Wang, Jibiao Wang, Dujun Wang, Xiaofeng Yu, Opeyemi Joshua Olatunji, Zhen Ouyang, Yuan Wei
The current study was aimed at investigating the neuroprotective effects of the butanol fraction from C. cicadae (CBU ), which was responsible for the anti-aging effect of this medicine. Glutamate-induced PC12 cells were used as a model to determine the neuroprotective effect against oxidative cell death. Cell viability, cytotoxicity, flow cytometry, mitochondrial transmembrane potential (MMP), reactive oxygen species (ROS), glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) levels were analyzed to assess neuronal cell survival or death...
November 7, 2017: Chemistry & Biodiversity
https://www.readbyqxmd.com/read/29111371/pharmacodynamics-in-alzheimer-s-disease-model-rats-of-a-bifunctional-peptide-with-the-potential-to-accelerate-the-degradation-and-reduce-the-toxicity-of-amyloid-%C3%AE-cu-fibrils
#17
Dan Wang, Qian Zhang, Xiaoyu Hu, Wei Wang, Xushan Zhu, Zhi Yuan
The accumulation of the extracellular β-amyloid (Aβ) aggregates with metal ions in conjunction with reactive oxygen species (ROS) is closely related to the pathogenesis of Alzheimer's disease (AD). Accounting on Cu ions chelating of our previously designed bifunctional peptide GGHRYYAAFFARR (GR) as well as Aβ-Cu fibrils (fAβ-Cu) dissociation potentials, we report herein an efficient route to synthetically minimize ROS toxicity and degrade fAβ-Cu. It is worth mentioning that GR combines the metal chelating agent GGH and β-sheet breaker RYYAAFFARR (RR)...
October 27, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/29107708/rab20-a-novel-rab-small-gtpase-that-negatively-regulates-neurite-outgrowth-of-pc12-cells
#18
Mai E Oguchi, Kan Etoh, Mitsunori Fukuda
The Rab family small GTPases are key players in the membrane traffic that underlies various cellular phenomena. Neurite outgrowth, which is a prerequisite for neuronal network formation, also requires membrane traffic from the cell body to the tips of neurites. Although several Rabs have been shown to promote neurite outgrowth, very little is known about Rab involvement in the negative regulation of neurite outgrowth. Here we used nerve growth factor-stimulated PC12 cells to perform siRNA-based comprehensive knockdown screenings for Rabs that negatively regulate neurite outgrowth and succeeded in identifying Rab20 as a novel negative regulator of neurite outgrowth...
October 28, 2017: Neuroscience Letters
https://www.readbyqxmd.com/read/29107423/multifunctional-iminochromene-2h-carboxamide-derivatives-containing-different-aminomethylene-triazole-with-bace1-inhibitory-neuroprotective-and-metal-chelating-properties-targeting-alzheimer-s-disease
#19
Aida Iraji, Omidreza Firuzi, Mehdi Khoshneviszadeh, Marjan Tavakkoli, Mohammad Mahdavi, Hamid Nadri, Najmeh Edraki, Ramin Miri
Alzheimer's disease (AD) is a neurodegenerative disorder known for the presence of amyloid beta plaques resulting from the sequential action of β-secretase and γ-secretase on amyloid precursor protein. We developed and synthesized, through click reactions, a new family of iminochromene carboxamides containing different aminomethylene triazole. The BACE1 inhibition, neuroprotective capacity and metal chelation of these derivatives make them ideal candidates against AD. Most of the synthesized compounds were shown to have potent BACE1 inhibitory activity in a FRET assay, with an IC50 value of 2...
December 1, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/29106600/chronic-irradiation-with-low-dose-rate-137cs-%C3%AE-rays-inhibits-ngf-induced-neurite-extension-of-pc12-cells-via-ca2-calmodulin-dependent-kinase-ii-activation
#20
Katoh Shinsuke, Kobayashi Junya, Umeda Tomonobu, Kobayashi Yoshiko, Nobuo Izumo, Suzuki Takahiko
Chronic irradiation with low-dose-rate 137Cs-γ rays inhibits the differentiation of human neural progenitor cells and influences the expression of proteins associated with several cellular functions. We aimed to determine whether such chronic irradiation influences the expression of proteins associated with PC12 cells. Chronic irradiation at 0.027 mGy/min resulted in inhibition of NGF-induced neurite extension. Furthermore, irradiation enhanced the nerve growth factor (NGF)-induced increase in the phosphorylation of extracellular signal-regulated kinase (ERK), but did not affect the phosphorylation of NGF receptors, suggesting that irradiation influences pathways unassociated with the activation of ERK...
June 30, 2017: Journal of Radiation Research
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