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https://www.readbyqxmd.com/read/28101189/inhibition-of-cytoskeletal-protein-carbonylation-may-protect-against-oxidative-damage-in-traumatic-brain-injury
#1
Qiusheng Zhang, Meng Zhang, Xianjian Huang, Xiaojia Liu, Weiping Li
Oxidative stress is the principal factor in traumatic brain injury (TBI) that initiates protracted neuronal dysfunction and remodeling. Cytoskeletal proteins are known to be carbonylated under oxidative stress; however, the complex molecular and cellular mechanisms of cytoskeletal protein carbonylation remain poorly understood. In the present study, the expression levels of glutathione (GSH) and thiobarbituric acid reactive substances (TBARS) were investigated in PC12 cells treated with H2O2. Western blot analysis was used to monitor the carbonylation levels of β-actin and β-tubulin...
December 2016: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28098758/neurotrophin-promotes-neurite-outgrowth-by-inhibiting-rif-gtpase-activation-downstream-of-mapks-and-pi3k-signaling
#2
Xiaoxia Tian, Huijuan Yan, Jiayi Li, Shuang Wu, Junyu Wang, Lifei Fan
Members of the well-known semaphorin family of proteins can induce both repulsive and attractive signaling in neural network formation and their cytoskeletal effects are mediated in part by small guanosine 5'-triphosphatase (GTPases). The aim of this study was to investigate the cellular role of Rif GTPase in the neurotrophin-induced neurite outgrowth. By using PC12 cells which are known to cease dividing and begin to show neurite outgrowth responding to nerve growth factor (NGF), we found that semaphorin 6A was as effective as nerve growth factor at stimulating neurite outgrowth in PC12 cells, and that its neurotrophic effect was transmitted through signaling by mitogen-activated protein kinases (MAPKs) and phosphatidylinositol-3-kinase (PI3K)...
January 13, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28093210/neuroprotection-by-paeoniflorin-in-the-mptp-mouse-model-of-parkinson-s-disease
#3
Meizhu Zheng, Chunming Liu, Yajun Fan, Pan Yan, Dongfang Shi, Yuchi Zhang
Paeoniflorin (PF) is a major bioactive ingredient in Radix Paeonia alba roots that has low toxicity and has been shown to have neuroprotective effects. Our in vitro experiments suggested that PF affords a significant neuroprotective effect against MPP(+)-induced damage and apoptosis in PC12 cells through Bcl-2/Bax/caspase-3 pathway. The objectives of the present study were to explore the potential neuroprotective effect of PF in 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP)-treated mouse model of Parkinson's disease (PD)...
January 13, 2017: Neuropharmacology
https://www.readbyqxmd.com/read/28078613/l-f001-a-multifunction-rock-inhibitor-prevents-6-ohda-induced-cell-death-through-activating-akt-gsk-3beta-and-nrf2-ho-1-signaling-pathway-in-pc12-cells-and-attenuates-mptp-induced-dopamine-neuron-toxicity-in-mice
#4
Liting Luo, Jingkao Chen, Dan Su, Meihui Chen, Bingling Luo, Rongbiao Pi, Lan Wang, Wei Shen, Rikang Wang
Amounting evidences demonstrated that Rho/Rho-associated kinase (ROCK) might be a novel target for the therapy of Parkinson's disease (PD). Recently, we synthesized L-F001 and revealed it was a potent ROCK inhibitor with multifunctional effects. Here we investigated the effects of L-F001 in PD models. We found that L-F001 potently attenuated 6-OHDA-induced cytotoxicity in PC12 cells and significantly decreased intracellular reactive oxygen species (ROS), prevented the 6-OHDA-induced decline of mitochondrial membrane potential and intracellular GSH levels...
January 11, 2017: Neurochemical Research
https://www.readbyqxmd.com/read/28077330/dehydropachymic-acid-decreases-bafilomycin-a1-induced-%C3%AE-amyloid-accumulation-in-pc12-cells
#5
Mengyao Yu, Xiaoyan Xu, Nan Jiang, Wei Wei, Fang Li, Liming He, Xia Luo
ETHNOPHARMACOLOGICAL RELEVANCE: Fuling, the sclerotium of Poria cocos, was frequently used in traditional Chinese medicine (TCM) formulae for Alzheimer's disease (AD) intervention over the past 10 centuries. And its extracts exhibited significant effects in both cellular and animal models of AD in previous studies. However, its mechanisms on prevention and treatment of AD have not been well elucidated yet. AIM OF THE STUDY: To investigate the effect and corresponding mechanisms of dehydropachymic acid, which is one of the major triterpenes in Poria cocos, on the clearance of β-Amyloid accumulation in bafilomycin A1 induced PC12 cells...
January 7, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28076410/role-for-egr1-in-the-transcriptional-program-associated-with-neuronal-differentiation-of-pc12-cells
#6
Kenneth W Adams, Sergey Kletsov, Ryan J Lamm, Jessica S Elman, Steven Mullenbrock, Geoffrey M Cooper
PC12 cells are a well-established model to study how differences in signal transduction duration can elicit distinct cell behaviors. Epidermal growth factor (EGF) activates transient ERK signaling in PC12 cells that lasts 30-60 min, which in turn promotes proliferation; nerve growth factor (NGF) activates more sustained ERK signaling that lasts 4-6 h, which in turns induces neuronal differentiation. Data presented here extend a previous study by Mullenbrock et al. (2011) that demonstrated that sustained ERK signaling in response to NGF induces preferential expression of a 69-member gene set compared to transient ERK signaling in response to EGF and that the transcription factors AP-1 and CREB play a major role in the preferential expression of several genes within the set...
2017: PloS One
https://www.readbyqxmd.com/read/28070104/comparative-photodynamic-therapy-cytotoxicity-of-mannose-conjugated-chlorin-and-talaporfin-sodium-in-cultured-human-and-rat-cells
#7
Yo Shinoda, Tsutomu Takahashi, Jiro Akimoto, Megumi Ichikawa, Hiromi Yamazaki, Atsushi Narumi, Shigenobu Yano, Yasuyuki Fujiwara
Photodynamic therapy (PDT) is a Food and Drug Administration authorized method for cancer treatment, which uses photosensitizer and laser photo-irradiation to generate reactive oxygen species to induce cell death in tumors. Photosensitizers have been progressively developed, from first to third generation, with improvements in cell specificity, reduced side effects and toxicity, increased sensitivity for irradiation and reduced persistence of photosensitizer in healthy cells. These improvements have been achieved by basic comparative experiments between current and novel photosensitizers using cell lines; however, photosensitizers should be carefully evaluated because they may have cell type specificity...
2017: Journal of Toxicological Sciences
https://www.readbyqxmd.com/read/28068609/dual-peptide-presenting-hydrogels-for-controlling-the-phenotype-of-pc12-cells
#8
Jae Won Lee, Kuen Yong Lee
Controlling the cell-matrix interaction is a critical factor in the design and fabrication of tissue engineering scaffolds. A particular peptide sequence, Arg-Gly-Asp (RGD peptide), is often used as an adhesion ligand in the engineering of different types of tissues. While in some cases this has been adequate, the use of multiple ligands may be required for the successful engineering of some tissue types. We hypothesized that hydrogels presenting both the RGD peptide and the YIGSR peptide (Tyr-Ile-Gly-Ser-Arg) could successfully regulate the phenotype of PC12 cells, thereby providing a new platform for effective tissue engineering applications...
January 3, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28049045/in-vitro-models-reveal-differences-in-the-developmental-neurotoxicity-of-an-environmental-polycylic-aromatic-hydrocarbon-mixture-compared-to-benzo-a-pyrene-neuronotypic-pc12-cells-and-embryonic-neural-stem-cells
#9
Theodore A Slotkin, Samantha Skavicus, Jennifer Card, Richard T Di Giulio, Frederic J Seidler
In addition to their carcinogenic activity, polycyclic aromatic hydrocarbons (PAHs) are suspected to be developmental neurotoxicants. We evaluated the effects of PAHs with two in vitro models that assess distinct "decision nodes" in neurodifferentiation: neuronotypic PC12 cells, which characterize the transition from cell replication to neurodifferentiation, neurite outgrowth and neurotransmitter specification; and embryonic neural stem cells (NSCs), which evaluate the origination of neurons and glia from precursors...
December 31, 2016: Toxicology
https://www.readbyqxmd.com/read/28045168/enhancing-electrochemical-detection-of-dopamine-via-dumbbell-like-fept-fe3o4-nanoparticles
#10
Weiwei Yang, Yongsheng Yu, Ying Tang, Keyun Li, Zheng Zhao, Menggang Li, Geping Yin, Haibo Li, Shouheng Sun
We synthesize dumbbell-like FePt-Fe3O4 nanoparticles (NPs) and study their electrocatalytic oxidation and sensing of dopamine (DA). The FePt and Fe3O4 NPs are strongly coupled in the dumbbell structure and as a result, the dumbbell FePt-Fe3O4 NPs show the best activity for DA oxidation with the DA detection limit reaching 1 nM. They are used successfully to monitor the DA concentration increase induced by K(+)-stimulation of PC12 cells. Our data show that an electrode modified with dumbbell-like FePt-Fe3O4 NPs can serve as the most sensitive probe for real-time quantitative detection of DA in a neurological environment...
January 19, 2017: Nanoscale
https://www.readbyqxmd.com/read/28044240/insulin-like-growth-factor-binding-protein6-associated-with%C3%A2-neuronal-apoptosis-following-intracerebral-hemorrhage-in-rats
#11
Qijun Dai, Peipei Jiang, Yang Gu, Lin Zhu, Haifeng Dai, Zhigang Yao, Hua Liu, Xiaoping Ma, Chengwei Duan, Lianxia Qu
The insulin-like growth factor (IGF) system is linked to CNS pathological states. The functions of IGFs are modulated by a family of binding proteins termed insulin-like growth factor binding proteins (IGFBPs). Here, we demonstrate that IGFBP-6 may be associated with neuronal apoptosis in the processes of intracerebral hemorrhage (ICH). We obtained a significant upregulation of IGFBP-6 in neurons adjacent to the hematoma following ICH with the results of Western blot, immunohistochemistry, and immunofluorescence...
January 2, 2017: Cellular and Molecular Neurobiology
https://www.readbyqxmd.com/read/28025799/upregulated-expression-of-trim32-is-involved-in-schwann-cell-differentiation-migration-and-neurite-outgrowth-after-sciatic-nerve-crush
#12
Yonghua Liu, Weijie Wu, Huiguang Yang, Zhengming Zhou, Xiaojian Zhu, Chi Sun, Yuxi Liu, Zhaohui Yu, Yuyan Chen, Youhua Wang
Tripartite motif containing 32 (TRIM32), a member of the tripartite motif (TRIM) family, plays an indispensable role in myoblast proliferation. It also regulates neuron and skeletal muscle stem cell differentiation. Although it is of great importance, we know little about the roles of TRIM32 during peripheral nervous system injury. Here, we examined the dynamic changes of TRIM32 in acute sciatic nerve crush (SNC) model. After crush, TRIM32 rapidly increased and reached the climax at 1 week but then gradually declined to the normal level at 4 weeks post-injury...
December 26, 2016: Neurochemical Research
https://www.readbyqxmd.com/read/28024531/presence-of-nano-sized-chitosan-layered-silicate-composites-protects-against-toxicity-induced-by-lead-ions
#13
Xinqin Liu, Rong Huang, Xue Zhou, Tongjian Cai, Jiajia Chen, Xiaowen Shi, Hongbing Deng, Wenjing Luo
Protecting cells from toxicosis even apoptosis induced by a variety of toxic heavy metals stimulus has drawn more and more attentions. This study was designed to elucidate whether chitosan-organic rectorite (CS-OREC) composites exhibited any protective effects on altered oxidative stress parameter in PC12 cells exposed to lead ions (Pb(2+)). The cells were exposed to Pb(2+) either alone or in combination with CS-OREC composites for designated time to evaluate the efficacy of the composites on Pb(2+)-induced toxicity...
February 20, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28017835/aav-mediated-transfer-of-rhoa-shrna-and-cntf-promotes-retinal-ganglion-cell-survival-and-axon-regeneration
#14
Ling-Ping Cen, Jia-Jian Liang, Jian-Huan Chen, Alan R Harvey, Tsz Kin Ng, Mingzhi Zhang, Chi Pui Pang, Qi Cui, You-Ming Fan
The aim of the present study was to determine whether adeno-associated viral vector (AAV) mediated transfer of ciliary neurotrophic factor (CNTF) and RhoA shRNA has additive effects on promoting the survival and axon regeneration of retinal ganglion cells (RGCs) after optic nerve crush (ONC). Silencing effects of AAV-RhoA shRNA were confirmed by examining neurite outgrowth in PC12 cells, and by quantifying RhoA expression levels with western blotting. Young adult Fischer rats received an intravitreal injection of (i) saline, (ii) AAV green fluorescent protein (GFP), (iii) AAV-CNTF, (iv) AAV-RhoA shRNA, or (v) a combination of both AAV-CNTF and AAV-RhoA shRNA...
December 23, 2016: Neuroscience
https://www.readbyqxmd.com/read/28007781/transient-receptor-potential-vanilloid-2-activation-by-focal-mechanical-stimulation-requires-interaction-with-the-actin-cytoskeleton-and-enhances-growth-cone-motility
#15
Shouta Sugio, Masami Nagasawa, Itaru Kojima, Yasuki Ishizaki, Koji Shibasaki
We have previously reported that transient receptor potential vanilloid 2 (TRPV2) can be activated by mechanical stimulation, which enhances axonal outgrowth in developing neurons; however, the molecular mechanisms that govern the contribution of TRPV2 activation to axonal outgrowth remain unclear. In the present study, we examined this mechanism by using PC12 cells as a neuronal model. Overexpression of TRPV2 enhanced axonal outgrowth in a mechanical stimulus-dependent manner. Accumulation of TRPV2 at the cell surface was 4-fold greater in the growth cone compared with the soma...
December 22, 2016: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/28007001/antioxidant-and-neuroprotector-effect-of-lepidium-meyenii-maca-methanol-leaf-extract-against-6-hydroxy-dopamine-6-ohda-induced-toxicity-in-pc12-cells
#16
Ángel Rodríguez-Huamán, Sandra Casimiro-Gonzales, Jorge Antonio Chávez-Pérez, Carla Gonzales-Arimborgo, Richard Cisneros-Fernández, Luis Ángel Aguilar-Mendoza, Gustavo F Gonzales
Reactive oxygen species (ROS) are normally produced during cell metabolism, there is strong evidence to suggest that ROS produced in excess impair the cell and may be etiologically related to various neurodegenerative diseases. This study was undertaken to examine the effects of Lepidium meyenii (MACA) methanol leaf extract on neurotoxicity in PC12 cell exposed to 6-hydroxydopamine (6-OHDA). Fresh samples of "maca" leaves were processed in order to obtain foliar extracts and to evaluate the neurobiological activity on PC12 cells, subjected to the cytotoxic effect of 6-OHDA through the determination of the capacity antioxidant, cell viability and cytotoxicity assays on PC12 cells...
January 8, 2017: Toxicology Mechanisms and Methods
https://www.readbyqxmd.com/read/28004763/tyrosine-hydroxylase-binding-to-phospholipid-membranes-prompts-its-amyloid-aggregation-and-compromises-bilayer-integrity
#17
Anne Baumann, Ana Jorge-Finnigan, Kunwar Jung-Kc, Alexander Sauter, Istvan Horvath, Ludmilla A Morozova-Roche, Aurora Martinez
Tyrosine hydroxylase (TH), a rate-limiting enzyme in the synthesis of catecholamine neurotransmitters and hormones, binds to negatively charged phospholipid membranes. Binding to both large and giant unilamellar vesicles causes membrane permeabilization, as observed by efflux and influx of fluorescence dyes. Whereas the initial protein-membrane interaction involves the N-terminal tail that constitutes an extension of the regulatory ACT-domain, prolonged membrane binding induces misfolding and self-oligomerization of TH over time as shown by circular dichroism and Thioflavin T fluorescence...
December 22, 2016: Scientific Reports
https://www.readbyqxmd.com/read/28003585/cold-stimulation-evokes-exocytotic-vesicle-release-from-pc12-cells
#18
Ryoichi Yoshimura, Misaki Nakagawa, Yasuhisa Endo
The mechanism of peripheral blood circulation failure by low temperatures, known as Raynaud's phenomenon, is not well understood at present. We examined the possibility that the efferent fibers of peripheral sympathetic neurons may release transmitters directly by cold sensation. PC12 cells, a culture model of sympathetic neurons, were placed at 25°C for 30 min, fixed with the tannic acid-glutaraldehyde-osmium method and observed by electron microscopy. The number of omega-shaped exocytotic vesicle figures increased significantly compared with that in the control (37°C), although it was lower than that of the positive control (high potassium stimulation at 25°C and 37°C)...
2016: Biomedical Research
https://www.readbyqxmd.com/read/27997906/effects-of-leptin-on-na-ca2-exchanger-in-pc12-cells
#19
Zijuan Zhang, Shuguang Sun, Caixia Du, Wei Li, Juan Zhang, Yanqin Zhu, Peilin Liu, Ying Xing
BACKGROUND/AIMS: Alzheimer's disease (AD) is known to be related to alterations in neuronal intracellular calcium activity ([Ca2+]i). The present study revealed the distinct role of leptin in Na+/Ca2+-exchanger activity. METHODS: [Ca2+]i was determined utilizing Fura-2 fluorescence. The activity of NCX was measured by removal of extracellular Na+ in the presence of external Ca2+. Na+/Ca2+-exchanger activity was further quantified from whole cell currents following removal of extracellular Na+...
2016: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/27988398/control-of-stress-induced-depressive-disorders-by-so-ochim-tang-gamibang-a-korean-herbal-medicine
#20
Jung Eun Choi, Dae-Myung Park, Eunho Chun, Jeong June Choi, Ji Hye Seo, Seunghyung Kim, Jaemin Son, Moonho Do, Sun Yeou Kim, Yang-Chun Park, In Chul Jung, Mirim Jin
ETHNOPHARMACOLOGICAL RELEVANCE: So-ochim-tang-gamibang (SOCG) is a Korean herbal medicine formula that has been applied to treat depressive moods and depression associated somatoform pain. This decoction consists of Cyperus rotundus L. (Cyperi Rhizoma), Lindera aggregata (Sims) Kosterm. (Linderae Radix), Aquilaria agallochum (Lour.) Roxb. ex Finl. (Aquilariae Resinatum Lignum), Glycyrrhiza uralensis Fisch. (Glycyrrhizae Radix) Platycodon grandiflorum (Jacq.) A. DC. (Platycodi Radix), and Citrus aurantium L...
January 20, 2017: Journal of Ethnopharmacology
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