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https://www.readbyqxmd.com/read/28810585/glutamine-promotes-hsp70-and-inhibits-%C3%AE-synuclein-accumulation-in-pheochromocytoma-pc12-cells
#1
Haiyang Wang, Chongyang Tang, Zhenfeng Jiang, Xiao Zhou, Jianhang Chen, Meng Na, Hong Shen, Zhiguo Lin
Hsp70 regulates α-Synuclein (α-Syn) degeneration in Parkinson's disease (PD), indicating that Hsp70 promotion may be able to prevent or reverse α-Syn-induced toxicity in PD. Additionally, it has been demonstrated that glutamine (Gln) enhances Hsp70 expression. In the present study, Gln-induced Hsp70 promotion in pheochromocytoma was investigated with reverse transcription- quantitative polymerase chain reaction and western blotting methods. Then it was observed whether heat shock factor (HSF)-1 was required for this phenomenon with an RNA interference strategy...
August 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28809765/cyclin-dependent-kinase-5-modulates-the-p2x2a-receptor-channel-gating-through-phosphorylation-of-c-terminal-threonine-372
#2
Claudio Coddou, Rodrigo Sandoval, Patricio Castro, Pablo Lazcano, Maria José Hevia, Milos Rokic, Bradford Hall, Anita Terse, Christian Gonzalez-Billault, Ashok B Kulkarni, Stanko S Stojilkovic, Elias Utreras
The purinergic P2X2 receptor (P2X2R) is an adenosine triphosphate-gated ion channel widely expressed in the nervous system. Here, we identified a putative cyclin-dependent kinase 5 (Cdk5) phosphorylation site in the full-size variant P2X2aR (TPKH), which is absent in the splice variant P2X2bR. We therefore investigated the effects of Cdk5 and its neuronal activator, p35, on P2X2aR function. We found an interaction between P2X2aR and Cdk5/p35 by co-immunofluorescence and co-immunoprecipitation in HEK293 cells...
July 17, 2017: Pain
https://www.readbyqxmd.com/read/28807675/substantial-protection-against-mptp-associated-parkinson-s-neurotoxicity-in-vitro-and-in-vivo-by-anti-cancer-agent-su4312-via-activation-of-mef2d-and-inhibition-of-mao-b
#3
Baojian Guo, Shengquan Hu, Chengyou Zheng, Hongyu Wang, Fangcheng Luo, Haitao Li, Wei Cui, Xifei Yang, Guozhen Cui, Shinghung Mak, Tony Chung-Lit Choi, Edmond Dik-Lung Ma, Yuqiang Wang, Simon Ming Yuen Lee, Zaijun Zhang, Yifan Han
We have previously demonstrated the unexpected neuroprotection of the anti-cancer agent SU4312 in cellular models associated with Parkinson's disease (PD). However, the precise mechanisms underlying its neuroprotection are still unknown, and the effects of SU4312 on rodent models of PD have not been characterized. In the current study, we found that the protection of SU4312 against 1-methyl-4-phenylpyridinium ion (MPP(+))-induced neurotoxicity in PC12 cells was achieved through the activation of transcription factor myocyte enhancer factor 2D (MEF2D), as evidenced by the fact that SU4312 stimulated myocyte enhancer factor 2 (MEF2) transcriptional activity and prevented the inhibition of MEF2D protein expression caused by MPP(+), and that short hairpin RNA (ShRNA)-mediated knockdown of MEF2D significantly abolished the neuroprotection of SU4312...
August 11, 2017: Neuropharmacology
https://www.readbyqxmd.com/read/28807223/gts40-an-active-fraction-of-gou-teng-san-gts-protects-pc12-from-h2o2-induced-cell-injury-through-antioxidative-properties
#4
Lei Chen, Meng-Lin Wei, Jiao-Jiao Zhao, Hao Hong, Wei Qu, Feng Feng, Wen-Yuan Liu
Oxidative stress, a predominant cause of apoptosis cascades triggered in neurodegenerative disorders, has been regarded as a critical inducement in the pathogenesis of Alzheimer's disease (AD). Gou Teng-San (GTS) is a traditional Chinese herbs preparation commonly utilized to alleviate cognitive dysfunction and psychological symptoms of patients with dementia. The present study aimed to investigate the protective effects of GTS40, an active fraction of GTS, on H2O2-induced oxidative damage and identify the potential active ingredients...
July 2017: Chinese Journal of Natural Medicines
https://www.readbyqxmd.com/read/28804614/protective-effect-of-bioactive-compounds-from-echinophora-cinerea-against-cisplatin-induced-oxidative-stress-and-apoptosis-in-the-pc12-cell-line
#5
Yald Shokoohinia, Shekoufeh Khajouei, Farahnaz Ahmadi, Nastaran Ghiasvand, Leila Hosseinzadeh
OBJECTIVES: The present study aims to evaluate the protective effect of the compounds isolated from Echinophora cinerea (E. cinerea) against oxidative stress and apoptosis induced by cisplatin (CIS) in PC12 cells. MATERIALS AND METHODS: Six compounds were isolated as quercetrin-3-O-β-D-glucopyranoside (QUE), osthol (OST), verbenone-5-O-β-D-glycopyranoside (VER), Isoimperatorin (ISO), kaempferol-3-O-β-D-glucopyranoside (KAM), and echinophorin B (ECH). For this study, we used MTT reduction assay for detection of protective effects of isolated compounds on CIS-induced cytotoxicity in PC12 cells...
April 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/28803044/new-racemic-annulated-pyrazolo-1-2-b-phthalazines-as-tacrine-like-ache-inhibitors-with-potential-use-in-alzheimer-s-disease
#6
Leili Jalili-Baleh, Hamid Nadri, Alireza Moradi, Syed Nasir Abbas Bukhari, Mojtaba Shakibaie, Mandana Jafari, Mostafa Golshani, Farshad Homayouni Moghadam, Loghman Firoozpour, Ali Asadipour, Saeed Emami, Mehdi Khoobi, Alireza Foroumadi
A novel series of tacrine-like compounds 7a-u possessing a fused pyrazolo[1,2-b]phthalazine structure were designed and synthesized as potent and selective inhibitors of AChE. The in-vitro biological assessments demonstrated that several compounds had high anti-AChE activity at nano-molar level. The more promising compound 7l with IC50 of 49 nM was 7-fold more potent than tacrine and unlike tacrine, it was highly selective against AChE over BuChE. The cell-based assays against hepatocytes (HepG2) and neuronal cell line (PC12) revealed that 7l had significantly lower hepatotoxicity compared to tacrine, with additional neuroprotective activity against H2O2-induced damage in PC12 cells...
July 31, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28801605/luteolin-a-natural-flavonoid-inhibits-methylglyoxal-induced-apoptosis-via-the-mtor-4e-bp1-signaling-pathway
#7
Yi Liu, Jie Huang, Xian Zheng, Xia Yang, Yan Ding, Tongyong Fang, Yuyun Zhang, Shuaishuai Wang, Xiaofei Zhang, Xuan Luo, Anlei Guo, Kelly A Newell, Yinghua Yu, Xu-Feng Huang
Methylglyoxal (MG) accumulation has been observed in human cerebrospinal fluid and body tissues under hyperglycaemic conditions. Recent research has demonstrated that MG-induces neuronal cell apoptosis, which promotes the development of diabetic encephalopathy. Our previous animal study has shown that luteolin, a natural flavonoid, attenuates diabetes-associated cognitive dysfunction. To further explore the neuroprotective properties of luteolin, we investigated the inhibitive effect of luteolin on MG-induced apoptosis in PC12 neuronal cells...
August 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28800459/novel-cinnamamide-dibenzylamine-hybrids-potent-neurogenic-agents-with-antioxidant-cholinergic-and-neuroprotective-properties-as-innovative-drugs-for-alzheimer-s-disease
#8
Jin Wang, Pei Cai, Xue-Lian Yang, Fan Li, Jia-Jia Wu, Ling-Yi Kong, Xiao-Bing Wang
By using fragments endowed with interesting and complementary properties for the treatment of Alzheimer's disease (AD), a novel series of cinnamamide-dibenzylamine hybrids have been designed, synthesized, and evaluated biologically. In vitro assay indicated that most of the target compounds exhibited a significant ability to inhibit ChEs, strong potency inhibitory of self-induced β-amyloid (Aβ) aggregation and to act as potential antioxidants and biometal chelators. A Lineweaver-Burk plot and molecular modeling study showed that compound 7f targeted both the CAS and PAS of AChE...
August 1, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28800073/electronegative-low-density-lipoprotein-l5-impairs-viability-and-ngf-induced-neuronal-differentiation-of-pc12-cells-via-lox-1
#9
Jiz-Yuh Wang, Chiou-Lian Lai, Ching-Tien Lee, Chen-Yen Lin
There have been striking associations of cardiovascular diseases (e.g., atherosclerosis) and hypercholesterolemia with increased risk of neurodegeneration including Alzheimer's disease (AD). Low-density lipoprotein (LDL), a cardiovascular risk factor, plays a crucial role in AD pathogenesis; further, L5, a human plasma LDL fraction with high electronegativity, may be a factor contributing to AD-type dementia. Although L5 contributing to atherosclerosis progression has been studied, its role in inducing neurodegeneration remains unclear...
August 11, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28799615/forsythenethosides-a-and-b-two-new-phenylethanoid-glycosides-with-a-15-membered-ring-from-forsythia-suspensa
#10
Si-Yuan Shao, Zi-Ming Feng, Ya-Nan Yang, Jian-Shuang Jiang, Pei-Cheng Zhang
Forsythenethosides A (1) and B (2), two new phenylethanoid glycosides with an unprecedented 15-membered carbon scaffold ring, were isolated from the fruits of Forsythia suspensa. Their structures, including their geometric configurations, were determined via extensive NMR spectroscopy techniques, especially using 2D NMR data combined with systematic conformational analysis. Forsythenethosides A and B showed strong neuroprotective activities against serum-deprivation-induced PC12 cell damage. Furthermore, they were active on rotenone-induced PC12 cell damage...
August 11, 2017: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/28797883/design-synthesis-and-biological-evaluation-of-novel-coumarin-n-benzyl-pyridinium-hybrids-as-multi-target-agents-for-the-treatment-of-alzheimer-s-disease
#11
Jin-Shuai Lan, Yue Ding, Yun Liu, Ping Kang, Jian-Wei Hou, Xin-Yu Zhang, Sai-Sai Xie, Tong Zhang
Combining N-benzyl pyridinium moiety and coumarin into in a single molecule, novel hybrids with ChE and MAO-B inhibitory activities were designed and synthesized. The biological screening results indicated that most of compounds displayed potent inhibitory activity for ChE and Aβ (1-42) self-aggregation, and clearly selective inhibition to MAO-B over MAO-A. Of these compounds, compound 7f was the most potent inhibitor for hMAO-B, and it was also a good and balanced inhibitor to ChEs and hMAO-B (0.0373 μM for eeAChE; 2...
July 24, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28792609/egcg-attenuates-fe-iii-induced-conformational-transition-of-%C3%AE-synuclein-and-protects-as-pc12-cells-against-fe-iii-induced-death
#12
Juan Zhao, Lihui Xu, Qingnan Liang, Qing Sun, Congheng Chen, Yuan Zhang, Yu Ding, Ping Zhou
The fibrillation and aggregation of α-synuclein (AS), along with the conformational transition from random coil to β-sheet, are the critical steps in the development of Parkinson's disease (PD). It is acknowledged that iron accumulation in the brain may lead to the fibrillation of AS. However, (-)-epigallocatechin gallate (EGCG) can penetrate the blood-brain barrier, chelate metal ions and inhibit the fibrillation of amyloid proteins. Therefore, EGCG is warranted to be investigated for its potential to cure amyloid-related diseases...
August 9, 2017: Journal of Neurochemistry
https://www.readbyqxmd.com/read/28791345/nova1-mediates-resistance-of-rat-pheochromocytoma-cells-to-hypoxia-induced-apoptosis-via-the-bax-bcl-2-caspase-3-pathway
#13
Hualing Li, Bei Lv, Ling Kong, Jing Xia, Ming Zhu, Lijuan Hu, Danyang Zhen, Yifan Wu, Xiaoqin Jia, Sujuan Zhu, Hengmi Cui
Neuro-oncological ventral antigen 1 (Nova1) is a well known brain-specific splicing factor. Several studies have identified Nova1 as a regulatory protein at the top of a hierarchical network. However, the function of Nova1 during hypoxia remains poorly understood. This study aimed to investigate the protective effect of Nova1 against cell hypoxia and to further explore the Bax/Bcl-2/caspase-3 pathway as a potential mechanism. During hypoxia, the survival rate of pheochromocytoma PC12 cells was gradually decreased and the apoptosis rate was gradually increased, peaking at 48 h of hypoxia...
August 3, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28776974/mechanistic-aspects-of-vesicle-opening-during-analysis-with-vesicle-impact-electrochemical-cytometry
#14
Xianchan Li, Johan Dunevall, Lin Ren, Andrew G Ewing
Vesicle impact electrochemical cytometry (VIEC) has been used to quantify the vesicular transmitter content in mammalian vesicles. In the present study, we studied the mechanism of VIEC by quantifying the catecholamine content in single vesicles isolated from pheochromocytoma (PC12) cells. These vesicles contain about one tenth of the catecholamine compared with adrenal chromaffin vesicles. The existence of a pre-spike foot for many events suggests the formation of an initial transiently stable pore at the beginning of vesicle rupture...
August 4, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28774849/comparison-of-different-in-vitro-cell-models-for-the-assessment-of-pesticide-induced-dopaminergic-neurotoxicity
#15
Harm J Heusinkveld, Remco H S Westerink
Biomedical and (neuro) toxicity research on (neuro) degenerative diseases still relies strongly on animal models. However, the use of laboratory animals is often undesirable for both ethical and technical reasons. Current in vitro research thus largely relies on tumor derived- or immortalized cell lines. Notably, the suitability of cell lines for studying neurodegeneration is determined by their intrinsic properties. We therefore characterized PC12, SH-SY5Y, MES23.5 and N27 cells with respect to the presence of functional membrane ion channels and receptors as well as for the effects of five known neurotoxic pesticides on cytotoxicity, oxidative stress and parameters of intracellular calcium homeostasis using a combined alamar Blue/CFDA assay, a H2DCFDA assay and single cell fluorescent (Fura-2) calcium imaging, respectively...
July 31, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28773847/mechanical-and-electronic-properties-of-xc%C3%A2-and-xc12
#16
Qun Wei, Quan Zhang, Meiguang Zhang
A series of carbon-based superconductors XC₆ with high Tc were reported recently. In this paper, based on the first-principles calculations, we studied the mechanical properties of these structures, and further explored the XC12 phases, where the X atoms are from elemental hydrogen to calcium, except noble gas atoms. The mechanically- and dynamically-stable structures include HC₆, NC₆, and SC₆ in XC₆ phases, and BC12, CC12, PC12, SC12, ClC12, and KC12 in XC12 phases. The doping leads to a weakening in mechanical properties and an increase in the elastic anisotropy...
August 25, 2016: Materials
https://www.readbyqxmd.com/read/28770951/microrna-181c-functions-as-a-protective-factor-in-a-1-methyl-4-phenylpyridinium-iodide-induced-cellular-parkinson-s-disease-model-via-bcl2l11
#17
M Wei, L-J Cao, J-L Zheng, L-J Xue, B Chen, F Xiao, C-S Xu
OBJECTIVE: Parkinson is one of the most common neurodegenerative diseases. At present, many studies have pointed out that miRNAs play a very important role in Parkinson's development and process. MiR-181c has been shown to have a significant low expression in blood samples and brain tissues of Parkinson's patients. MATERIALS AND METHODS: We used 1-Methyl-4-Phenylpyridinium Iodide (MPP(+)) as a tool for constructing the Parkinson's cell model, using mir181c mimics to construct an experimental model of acquisition...
July 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28768144/the-c-terminal-domain-of-zdhhc2-contains-distinct-sorting-signals-that-regulate-intracellular-localisation-in-neurons-and-neuroendocrine-cells
#18
Christine Salaun, Louise Ritchie, Jennifer Greaves, Trevor J Bushell, Luke H Chamberlain
The S-acyltransferase zDHHC2 mediates dynamic S-acylation of PSD95 and AKAP79/150, which impacts synaptic targeting of AMPA receptors. zDHHC2 is responsive to synaptic activity and catalyses the increased S-acylation of PSD95 that occurs following action potential blockade or application of ionotropic glutamate receptor antagonists. These treatments have been proposed to increase plasma membrane delivery of zDHHC2 via an endosomal cycling pathway, enhancing substrate accessibility. To generate an improved understanding of zDHHC2 trafficking and how this might be regulated by neuronal activity, we searched for intramolecular signals that regulate enzyme localisation...
July 30, 2017: Molecular and Cellular Neurosciences
https://www.readbyqxmd.com/read/28762191/inhibition-of-autophagy-is-involved-in-the-protective-effects-of-ginsenoside-rb1-on-spinal-cord-injury
#19
Peng Wang, Chaowei Lin, Shiyang Wu, Kelun Huang, Yu Wang, Xiaomei Bao, Fan Zhang, Zhihui Huang, Honglin Teng
Spinal cord injury (SCI) is a devastating neurological disorder. Autophagy is induced and plays a crucial role in SCI. Ginsenoside Rb1 (Rb1), one of the major active components extracted from Panax Ginseng CA Meyer, has exhibited neuroprotective effects in various neurodegenerative diseases. However, it remains unknown whether autophagy is involved in the neuroprotection of Rb1 on SCI. In this study, we examined the regulation of autophagy following Rb1 treatment and its involvement in the Rb1-induced neuroprotection in SCI and in vitro injury model...
July 31, 2017: Cellular and Molecular Neurobiology
https://www.readbyqxmd.com/read/28758954/chemical-constituents-from-hericium-erinaceus-promote-neuronal-survival-and-potentiate-neurite-outgrowth-via-the-trka-erk1-2-pathway
#20
Cheng-Chen Zhang, Chen-Yu Cao, Miwa Kubo, Kenichi Harada, Xi-Tao Yan, Yoshiyasu Fukuyama, Jin-Ming Gao
Hericium erinaceus is a culinary-medicinal mushroom used traditionally in Eastern Asia to improve memory. In this work, we investigated the neuroprotective and neuritogenic effects of the secondary metabolites isolated from the MeOH extract of cultured mycelium of H. erinaceus and the primary mechanisms involved. One new dihydropyridine compound (6) and one new natural product (2) together with five known compounds (1,3-5,7) were obtained and their structures were elucidated by spectroscopic analysis, including 2D NMR and HRMS...
July 30, 2017: International Journal of Molecular Sciences
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