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https://www.readbyqxmd.com/read/27910893/interference-with-protease-activated-receptor-1-alleviates-neuronal-cell-death-induced-by-lipopolysaccharide-stimulated-microglial-cells-through-the-pi3k-akt-pathway
#1
Yuxin Li, Wuyang Yang, Alfredo Quinones-Hinojosa, Baocheng Wang, Shujun Xu, Weijie Zhu, Feng Yu, Shaoji Yuan, Peigang Lu
Excessive microglial cells activation in response to inflammatory stimuli leads to synaptic loss, dysfunction, and neuronal cell death. Activated microglia are involved in the pathogenesis of neurological conditions and frequently contribute to several complications. Accumulating evidence suggests that signaling through PAR-1 is involved in inflammation, however, its function has yet to be fully elucidated. Here, we have demonstrated that the suppression of PAR-1 leads to down-regulation of inflammatory factors including IL-1β, IL-6, TNF-α, NO, as well as the prevention of activation of NF-κB in BV2 cells...
December 2, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27905509/the-er-stress-regulator-bip-mediates-cadmium-induced-autophagy-and-neuronal-senescence
#2
Tao Wang, Yan Yuan, Hui Zou, Jinlong Yang, Shiwen Zhao, Yonggang Ma, Yi Wang, Jianchun Bian, Xuezhong Liu, Jianhong Gu, Zongping Liu, Jiaqiao Zhu
Autophagy is protective in cadmium (Cd)-induced oxidative damage. Endoplasmic reticulum (ER) stress has been shown to induce autophagy in a process requiring the unfolded protein response signalling pathways. Cd treatment significantly increased senescence in neuronal cells, which was aggravated by 3-MA or silencing of Atg5 and abolished by rapamycin. Cd increased expression of ER stress regulators Bip, chop, eIf2α, and ATF4, and activated autophagy as evidenced by upregulated LC3. Moreover, the ER stress inhibitor mithramycin inhibited the expression of ER stress protein chaperone Bip and blocked autophagic flux...
December 1, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27894045/scintillation-imaging-of-tritium-radioactivity-distribution-during-tritiated-thymidine-uptake-by-pc12-cells-using-a-melt-on-scintillator
#3
Namiko Irikura, Hirokazu Miyoshi, Yasuo Shinohara
A scintillation image of tritium fixed in a melt-on scintillator was obtained using a charged-coupled device (CCD) imager, and a linear relationship was observed between the intensity of the scintillation image and the radioactivity of tritium. In a [(3)H]thymidine uptake experiment, a linear correlation between the intensity of the CCD image and the dilution ratio of cells was confirmed. Scintillation imaging has the potential for use in direct observation of tritium radioactivity distribution.
November 15, 2016: Applied Radiation and Isotopes
https://www.readbyqxmd.com/read/27893608/ape1-protects-against-mpp-induced-neurotoxicity-through-erk1-2-signaling-in-pc12-cells
#4
Bei Kang, Shengzhi Mu, Qian Yang, Shenglong Guo, Xiaoli Chen, Hena Guo
Oxidative stress, induced by reactive oxygen species (ROS), is an apoptosis activator. Oxidative stress causes dopaminergic neuron loss and plays a pivotal role in the pathogenesis of Parkinson's disease (PD). A recent study showed that apurinic/apyrimidinic endonuclease 1 (Ape1) decreases cytotoxicity and promotes neuron survival under oxidative stress. Furthermore, it has been proven that Ape1 is involved in the pathogenesis of PD. However, little is known about the contribution of Ape1 toward the development of PD...
January 1, 2017: Neuroreport
https://www.readbyqxmd.com/read/27893605/synergistic-combinations-of-five-single-drugs-from-centella-asiatica-for-neuronal-differentiation
#5
Jinjin Lin, Hui Jiang, Xianting Ding
To identify alternatives of nerve growth factor, which could promote NF68 protein expression and contribute toward neuronal differentiation, five compounds namely: asiatic acid, madecassic, madecassoside, quercetin, and isoquercetin, obtained from Centella asiatica, were examined for their neuronal differentiation effects on PC12 cells. C. asiatica has been applied as an effective herbal medicine for the treatment of various diseases, including depression. According to a statistical design of experiments, both single compound and compound combinations were evaluated...
January 1, 2017: Neuroreport
https://www.readbyqxmd.com/read/27891320/the-association-of-vdac-with-cell-viability-of-pc12-model-of-huntington-s-disease
#6
Andonis Karachitos, Daria Grobys, Klaudia Kulczyńska, Adrian Sobusiak, Hanna Kmita
It is becoming increasingly apparent that mitochondria dysfunction plays an important role in the pathogenesis of Huntington's disease (HD), but the underlying mechanism is still elusive. Thus, there is a still need for further studies concerning the upstream events in the mitochondria dysfunction that could contribute to cell death observed in HD. Taking into account the fundamental role of the voltage-dependent anion-selective channel (VDAC) in mitochondria functioning, it is reasonable to consider the channel as a crucial element in HD etiology...
2016: Frontiers in Oncology
https://www.readbyqxmd.com/read/27878303/neuroprotective-effects-of-vitexin-against-isoflurane-induced-neurotoxicity-by-targeting-the-trpv1-and-nr2b-signaling-pathways
#7
Linlin Chen, Bin Zhang, Shiqiang Shan, Xin Zhao
Vitexin is a bioactive compound extracted from hawthorn leaves, which reduces blood pressure and has anti‑inflammatory and potential anticancer effects. However, the mechanisms underlying the protective effects of vitexin against isoflurane‑induced neurotoxicity remain elusive. Therefore, the aim of the present study was to investigate these mechanisms further. Sprague Dawley rats received 1.4% isoflurane in a 100% oxygen environment for 2 h. Human PC12 pheochromocytoma neurosecretory cells were exposed to 2% isoflurane for 12 h before they were treated with 1, 10 or 100 µM vitexin for a further 24 h...
November 16, 2016: Molecular Medicine Reports
https://www.readbyqxmd.com/read/27877111/polymerizing-pyrrole-coated-poly-l-lactic-acid-co-%C3%AE%C2%B5-caprolactone-plcl-conductive-nanofibrous-conduit-combined-with-electric-stimulation-for-long-range-peripheral-nerve-regeneration
#8
Jialin Song, Binbin Sun, Shen Liu, Wei Chen, Yuanzheng Zhang, Chunyang Wang, Xiumei Mo, Junyi Che, Yuanming Ouyang, Weien Yuan, Cunyi Fan
Electrospinning and electric stimulation (ES) are both promising methods to support neuron adhesion and guide extension of neurons for nerve regeneration. Concurrently, all studies focus on either electrospinning for conduits material or ES in vitro study to accelerate nerve regeneration; few work on the combined use of these two strategies or ES in vivo study. Therefore, this study aimed to investigate the abilities of direct current ES through electrospinning conductive polymer composites composed of polypyrrole and Poly (l-lactic acid-co-ε-caprolactone) (PPY/PLCL) in peripheral nerve regeneration...
2016: Frontiers in Molecular Neuroscience
https://www.readbyqxmd.com/read/27877109/npas3-regulates-transcription-and-expression-of-vgf-implications-for-neurogenesis-and-psychiatric-disorders
#9
Dongxue Yang, Wenbo Zhang, Arshad Padhiar, Yao Yue, Yonghui Shi, Tiezheng Zheng, Kaspar Davis, Yu Zhang, Min Huang, Yuyuan Li, Li Sha
Neuronal PAS domain protein 3 (NPAS3) and VGF (VGF Nerve Growth Factor (NGF) Inducible) are important for neurogenesis and psychiatric disorders. Previously, we have demonstrated that NPAS3 regulates VGF at the transcriptional level. In this study, VGF (non-acronymic) was found regulated by NPAS3 in neuronal stem cells. However, the underlying mechanism of this regulation remains unclear. The aim of this study was to explore the correlation of NPAS3 and VGF, and their roles in neural cell proliferation, in the context of psychiatric illnesses...
2016: Frontiers in Molecular Neuroscience
https://www.readbyqxmd.com/read/27874976/etidronate-rescues-cognitive-deficits-through-improving-synaptic-transmission-and-suppressing-apoptosis-in-2-vessel-occlusion-model-rats
#10
Wen Li, Huijuan Yuan, Yao Yu, Yuen-Ki Cheong, Guogang Ren, Zhuo Yang
Vascular dementia (VD) is a neurodegenerative disorder caused by the reduction of cerebral blood flow. It shows a progressive cognitive impairment. In our previous study, we found that etidronate (ET) showed neuroprotective effects against glutamate-injured PC12 cells. Thus in this study, we aimed to observe the effects of ET on learning and memory impairment and the related mechanism in 2-vessel occlusion (2VO) model rats. Rats were administered a permanent bilateral common carotid artery occlusion to induce VD model...
November 22, 2016: Journal of Neurochemistry
https://www.readbyqxmd.com/read/27871898/the-cannabinoid-beta-caryophyllene-bcp-induces-neuritogenesis-in-pc12-cells-by-a-cannabinoid-receptor-independent-mechanism
#11
Neife Aparecida Guinaim Santos, Nádia Maria Martins, Flávia Malvestio Sisti, Laís Silva Fernandes, Rafaela Scalco Ferreira, Osvaldo de Freitas, Antônio Cardozo Santos
Beta-caryophyllene (BCP) is a phytocannabinoid whose neuroprotective activity has been mainly associated with selective activation of cannabinoid-type-2 (CB2) receptors, inhibition of microglial activation and decrease of inflammation. Here, we addressed the potential of BCP to induce neuritogenesis in PC12 cells, a model system for primary neuronal cells that express trkA receptors, respond to NGF and do not express CB2 receptors. We demonstrated that BCP increases the survival and activates the NGF-specific receptor trkA in NGF-deprived PC12 cells, without increasing the expression of NGF itself...
November 18, 2016: Chemico-biological Interactions
https://www.readbyqxmd.com/read/27863118/nanofibrous-gelatin-scaffolds-integrated-with-ngf-loaded-alginate-microspheres-for-brain-tissue-engineering
#12
Melda Büyüköz, Esra Erdal, Sacide Alsoy Altinkaya
Neural regeneration research is designed in part to develop strategies for therapy after nerve damage due to injury or disease. In this study, a new gelatin based biomimetic scaffold was fabricated for brain tissue engineering applications. A technique combining thermally induced phase separation and porogen leaching was used to create interconnected macropores and nanofibrous structure. To promote tissue regeneration processes, the scaffolds were integrated with nerve growth factor (NGF) loaded alginate microspheres...
November 12, 2016: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/27845038/hypoxia-pretreatment-and-epo-modification-enhance-the-protective-effects-of-msc-on-neuron-like-pc12%C3%A2-cells-in-a-similar-way
#13
Jinli Feng, Wei Wang
Mesenchymal stem cells (MSC) based cell transplantation therapy is proved to be an attractive strategy with great potential for improvement of hypoxia induced neural damage. In the present study, MSCs were co-culture with PC12 to investigate its protective effects against hypoxia pretreatment, and the Lactate dehydrogenase (LDH) release assay, MTT and Anexin V staining were performed to analysis the cellular damage or apoptotic. RT-PCR and Western blotting were further used to investigate the underlying mechanism...
November 11, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/27843479/protective-effect-of-psoralea-corylifolia-l-seed-extract-against-palmitate-induced-neuronal-apoptosis-in-pc12-cells
#14
Yunkyoung Lee, Hee-Sook Jun, Yoon Sin Oh
The extract of Psoralea corylifolia seeds (PCE) has been widely used as a herbal medicine because of its beneficial effect on human health. In this study, we investigated the protective effects and molecular mechanisms of PCE on palmitate- (PA-) induced toxicity in PC12 cells, a neuron-like cell line. PCE significantly increased cell viability in PA-treated PC12 cells and showed antiapoptotic effects, as evidenced by decreased expression of cleaved caspase-3, cleaved poly(ADP-ribose) polymerase, and bax protein as well as increased expression of bcl-2 protein...
2016: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/27843073/off-target-effect-of-sildenafil-on-postsurgical-erectile-dysfunction-alternate-pathways-and-localized-delivery-system
#15
Amirali Salmasi, Geun Taek Lee, Neal Patel, Ritu Goyal, Michael Dinizo, Young Suk Kwon, Part K Modi, Izak Faiena, Nara Lee, Johanna L Hannan, Joachim Kohn, Isaac Yi Kim
INTRODUCTION: There is no consensus on the best oral phosphodiesterase type 5 inhibitor (PDE5I) for patients undergoing penile rehabilitation after surgical nerve injury. AIM: To determine the mechanism of PDE5I on cultured neuronal cells and the effectiveness of local drug delivery using nanospheres (NSPs) to sites of nerve injury in a rat model of bilateral cavernous nerve injury (BCNI). METHODS: The effects of sildenafil, tadalafil, and vardenafil on cyclic adenosine monophosphate, cyclic guanosine monophosphate, and cell survival after exposure to hypoxia and H2O2 were measured in PC12, SH-SY5Y, and NTERA-2 (NT2) cell cultures...
November 11, 2016: Journal of Sexual Medicine
https://www.readbyqxmd.com/read/27835779/berberine-protects-against-6-ohda-induced-neurotoxicity-in-pc12-cells-and-zebrafish-through-hormetic-mechanisms-involving-pi3k-akt-bcl-2-and-nrf2-ho-1-pathways
#16
Chao Zhang, Chuwen Li, Shenghui Chen, Zhiping Li, Xuejing Jia, Kai Wang, Jiaolin Bao, Yeer Liang, Xiaotong Wang, Meiwan Chen, Peng Li, Huanxing Su, Jian-Bo Wan, Simon Ming Yuen Lee, Kechun Liu, Chengwei He
Berberine (BBR) is a renowned natural compound that exhibits potent neuroprotective activities. However, the cellular and molecular mechanisms are still unclear. Hormesis is an adaptive mechanism generally activated by mild oxidative stress to protect the cells from further damage. Many phytochemicals have been shown to induce hormesis. This study aims to investigate whether the neuroprotective activity of BBR is mediated by hormesis and the related signaling pathways in 6-OHDA-induced PC12 cells and zebrafish neurotoxic models...
November 4, 2016: Redox Biology
https://www.readbyqxmd.com/read/27829862/assessment-of-antioxidant-activity-and-neuroprotective-capacity-on-pc12-cell-line-of-frankenia-thymifolia-and-related-phenolic-lc-ms-ms-identification
#17
Rim Ben Mansour, Wided Megdiche Ksouri, Stéphanie Cluzet, Stéphanie Krisa, Tristan Richard, Riadh Ksouri
This work aimed to investigate the richness of a Tunisian xerohalophyte Frankenia thymifolia aerial and root parts on phenolics and to evaluate the antioxidant and neuroprotective properties of this medicinal species. After fractionation using increasing and different solvent polarities, results displayed five fractions, where ethyl acetate (EtOAc) shoot and root fractions possess considerable total phenolic contents (221 and 308 mg of GAE/g of E, resp.) related to their important antioxidant activities such as ORAC (918 and 713 mg of TE/g of E), DPPH (282 and 821 mg of TE/g), and ABTS (778 and 1320 mg of TE/g) tests...
2016: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/27823627/anti-neuroinflammatory-effect-of-sophoraflavanone-g-from-sophora-alopecuroides-in-lps-activated-bv2-microglia-by-mapk-jak-stat-and-nrf2-ho-1-signaling-pathways
#18
Chao Guo, Lei Yang, Chuan-Xing Wan, Yuan-Zheng Xia, Chao Zhang, Meng-Han Chen, Zhen-Dong Wang, Zhong-Rui Li, Xue-Mei Li, Ya-Di Geng, Ling-Yi Kong
BACKGROUND: Neuroinflammation plays a vital role in Alzheimer's disease (AD) and other neurodegenerative conditions. Sophora alopecuroides is widely used in traditional Uighur's medicine for the treatment of inflammation. Sophoraflavanone G (SG), a major flavonoid found in the S. alopecuroides, has also been reported to exhibit anti-inflammatory activity both in vitro and in vivo. However, the effect of S. alopecuroides and SG on microglia-mediated neuroinflammation has not been investigated...
December 1, 2016: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/27820974/oroxylin-a-inhibits-h2o2-induced-oxidative-stress-in-pc12-cells
#19
Qinghe Han, Haiyu Wang, Chong Xiao, Ben-Dong Fu, Chong-Tao Du
Oroxylin A, a natural flavonoid isolated from Scutellaria baicalensis, has been reported to have anti-inflammatory and antioxidant effects. However, suppression of oxidative stress and neuroapoptosis by oroxylin A is largely uninvestigated. To investigate the protective effects of oroxylin A, PC12 cells were exposed to oroxylin A and hydrogen peroxide solutions and measured. Oroxylin A significantly reduced the levels of intracellular calcium and reactive oxygen species and increased the levels of CAT and Mn/SOD...
November 7, 2016: Natural Product Research
https://www.readbyqxmd.com/read/27819177/an-investigation-of-the-developmental-neurotoxic-potential-of-curcumol-in-pc12-cells
#20
Chunlei Yu, Xiaojie Sun, Yingcai Niu
Curcuma phaeocaulis Val. is a Chinese medicinal herb that is contraindicated during pregnancy for over a thousand years in China. The aims of the present study were to evaluate the effect of curcumol (one of the major components of C. phaeocaulis Val.) on neurite outgrowth and characterize the signal transduction pathways in PC12 cells. Curcumol significantly inhibited neurite outgrowth and cell proliferation, but did not cause cell death at a concentration of 450 μM in differentiated PC12 cells. In addition, curcumol evoked oxidative stress and it was indicated by an elevation in reactive oxygen species (ROS) and lipid peroxidation (LPO)...
November 6, 2016: Toxicology Mechanisms and Methods
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