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https://www.readbyqxmd.com/read/28763026/genome-wide-analysis-reveals-extensive-changes-in-lncrnas-during-skeletal-muscle-development-in-hu-sheep
#1
Caifang Ren, Mingtian Deng, Yixuan Fan, Hua Yang, Guomin Zhang, Xu Feng, Fengzhe Li, Dan Wang, Feng Wang, Yanli Zhang
As an important type of noncoding RNA molecules, long non-coding RNAs (lncRNAs) act as versatile players in various biological processes. However, little is known about lncRNA regulators during sheep muscle growth. To explore functional lncRNAs during sheep muscle growth, we systematically investigated lncRNAs using strand-specific Ribo-Zero RNA sequencing at three key developmental stages in Hu sheep. A total of 6924 lncRNAs were obtained, and the differentially expressed lncRNAs and genes were screened from (control vs...
August 1, 2017: Genes
https://www.readbyqxmd.com/read/28706255/mitsugumin-29-regulates-t-tubule-architecture-in-the-failing-heart
#2
Robert N Correll, Jeffrey M Lynch, Tobias G Schips, Vikram Prasad, Allen J York, Michelle A Sargent, Didier X P Brochet, Jianjie Ma, Jeffery D Molkentin
Transverse tubules (t-tubules) are uniquely-adapted membrane invaginations in cardiac myocytes that facilitate the synchronous release of Ca(2+) from internal stores and subsequent myofilament contraction, although these structures become disorganized and rarefied in heart failure. We previously observed that mitsugumin 29 (Mg29), an important t-tubule organizing protein in skeletal muscle, was induced in the mouse heart for the first time during dilated cardiomyopathy with heart failure. Here we generated cardiac-specific transgenic mice expressing Mg29 to model this observed induction in the failing heart...
July 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28656064/epigenomic-and-functional-characterization-of-junctophilin-3-jph3-as-a-novel-tumor-suppressor-being-frequently-inactivated-by-promoter-cpg-methylation-in-digestive-cancers
#3
Xiaotong Hu, Yeye Kuang, Lili Li, Haimei Tang, Qinglan Shi, Xingsheng Shu, Yanjiao Zhang, Francis Kl Chan, Qian Tao, Chao He
Junctophilin (JPH) proteins stabilize junctional membrane complexes between plasma membrane and endoplasmic reticulum, also implicated in some human diseases. JPH3 mutations are linked to Huntington's disease-like 2 syndrome. Through epigenomic study of a colon cancer cell line pair (HCT116 and DKO), we identified JPH3 as a methylated novel tumor suppressor gene (TSG) candidate at 16q24. We further studied its epigenetic alterations and functions in digestive tumorigenesis. JPH3 expression at the RNA level was found to be frequently silenced or reduced in colorectal and gastric cancers due to its promoter CpG methylation, which is associated with tumor progression and poor survival of digestive cancer patients...
2017: Theranostics
https://www.readbyqxmd.com/read/28566298/colchicine-depolymerizes-microtubules-increases-junctophilin-2-and-improves-right-ventricular-function-in-experimental-pulmonary-arterial-hypertension
#4
Kurt W Prins, Lian Tian, Danchen Wu, Thenappan Thenappan, Joseph M Metzger, Stephen L Archer
BACKGROUND: Pulmonary arterial hypertension (PAH) is a lethal disease characterized by obstructive pulmonary vascular remodeling and right ventricular (RV) dysfunction. Although RV function predicts outcomes in PAH, mechanisms of RV dysfunction are poorly understood, and RV-targeted therapies are lacking. We hypothesized that in PAH, abnormal microtubular structure in RV cardiomyocytes impairs RV function by reducing junctophilin-2 (JPH2) expression, resulting in t-tubule derangements...
May 31, 2017: Journal of the American Heart Association
https://www.readbyqxmd.com/read/28473402/post-myocardial-infarction-t-tubules-form-enlarged-branched-structures-with-dysregulation-of-junctophilin-2-and-bridging-integrator-1-bin-1
#5
Christian Pinali, Nadim Malik, J Bernard Davenport, Laurence J Allan, Lucy Murfitt, Mohammad M Iqbal, Mark R Boyett, Elizabeth J Wright, Rachel Walker, Yu Zhang, Halina Dobryznski, Cathy M Holt, Ashraf Kitmitto
BACKGROUND: Heart failure is a common secondary complication following a myocardial infarction (MI), characterized by impaired cardiac contraction and t-tubule (t-t) loss. However, post-MI nano-scale morphological changes to the remaining t-ts are poorly understood. METHOD AND RESULTS: We utilized a porcine model of MI, using a nonlethal microembolization method to generate controlled microinfarcts. Using serial block face scanning electron microscopy, we report that post-MI, after mild left-ventricular dysfunction has developed, t-ts are not only lost in the peri-infarct region, but also the remnant t-ts form enlarged, highly branched disordered structures, containing a dense intricate inner membrane...
May 4, 2017: Journal of the American Heart Association
https://www.readbyqxmd.com/read/28447290/regulation-of-cardiomyocyte-t-tubular-structure-opportunities-for-therapy
#6
REVIEW
Ornella Manfra, Michael Frisk, William E Louch
PURPOSE OF REVIEW: Membrane invaginations called t-tubules play an integral role in triggering cardiomyocyte contraction, and their disruption during diseases such as heart failure critically impairs cardiac performance. In this review, we outline the growing understanding of the malleability of t-tubule structure and function, and highlight emerging t-tubule regulators which may be exploited for novel therapies. RECENT FINDINGS: New technologies are revealing the nanometer scale organization of t-tubules, and their functional junctions with the sarcoplasmic reticulum called dyads, which generate Ca(2+) sparks...
June 2017: Current Heart Failure Reports
https://www.readbyqxmd.com/read/28393127/novel-junctophilin-2-mutation-a405s-is-associated-with-basal-septal-hypertrophy-and-diastolic-dysfunction
#7
Ann P Quick, Andrew P Landstrom, Qiongling Wang, David L Beavers, Julia O Reynolds, Giselle Barreto-Torres, Viet Tran, Jordan Showell, Leonne E Philippen, Shaine A Morris, Darlene Skapura, J Martijn Bos, Steen E Pedersen, Robia G Pautler, Michael J Ackerman, Xander H T Wehrens
BACKGROUND: Hypertrophic cardiomyopathy (HCM), defined as asymmetric left ventricular hypertrophy, is a leading cause of cardiac death in the young. Perturbations in calcium (Ca(2+)) handling proteins have been implicated in the pathogenesis of HCM. JPH2-encoded junctophilin 2 is a major component of the junctional membrane complex, the subcellular microdomain involved in excitation-contraction coupling. We hypothesized that a novel JPH2 mutation identified in patients with HCM is causally linked to HCM, and alters intracellular Ca(2+) signaling in a pro-hypertrophic manner...
February 2017: JACC. Basic to Translational Science
https://www.readbyqxmd.com/read/28356340/analysis-of-cardiac-myocyte-maturation-using-casaav-a-platform-for-rapid-dissection-of-cardiac-myocyte-gene-function-in-vivo
#8
REVIEW
Yuxuan Guo, Nathan J VanDusen, Lina Zhang, Weiliang Gu, Isha Sethi, Silvia Guatimosim, Qing Ma, Blake D Jardin, Yulan Ai, Donghui Zhang, Biyi Chen, Ang Guo, Guo-Cheng Yuan, Long-Sheng Song, William T Pu
RATIONALE: Loss-of-function studies in cardiac myocytes (CMs) are currently limited by the need for appropriate conditional knockout alleles. The factors that regulate CM maturation are poorly understood. Previous studies on CM maturation have been confounded by heart dysfunction caused by whole organ gene inactivation. OBJECTIVE: To develop a new technical platform to rapidly characterize cell-autonomous gene function in postnatal murine CMs and apply it to identify genes that regulate transverse tubules (T-tubules), a hallmark of mature CMs...
June 9, 2017: Circulation Research
https://www.readbyqxmd.com/read/28062939/early-transverse-tubule-development-begins-in-utero-in-the-sheep-heart
#9
Michelle L Munro, Christian Soeller
The ventricular cardiomyocytes of adult mammals contain invaginations of the plasma membrane known as transverse (t)-tubules. These regular structures are essential for the synchronisation of excitation-contraction (EC) coupling throughout the cell, which is a vital process for cardiac function. T-tubules form a close association with the sarcoplasmic reticulum (SR) to form junctions, where several key proteins involved in EC coupling are localised, including the SR calcium release channels-the ryanodine receptors (RyR)...
January 6, 2017: Journal of Muscle Research and Cell Motility
https://www.readbyqxmd.com/read/27979982/role-of-calpain-in-eccentric-contraction-induced-proteolysis-of-ca2-regulatory-proteins-and-force-depression-in-rat-fast-twitch-skeletal-muscle
#10
Keita Kanzaki, Daiki Watanabe, Mai Kuratani, Takashi Yamada, Satoshi Matsunaga, Masanobu Wada
The aim of this study was to examine the in vivo effects of eccentric contraction (ECC) on calpain-dependent proteolysis of Ca(2+)-regulatory proteins and force production in fast-twitch skeletal muscles. Rat extensor digitorum longus muscles were exposed to 200-repeated ECC in situ and excised immediately (recovery 0) or 3 days (recovery 3) after cessation of ECC. Calpain inhibitor (CI)-treated rats were intraperitoneally injected with MDL-28170 before ECC and during 3 days of recovery. Tetanic force was markedly reduced at recovery 0 and remained reduced at recovery 3...
December 15, 2016: Journal of Applied Physiology
https://www.readbyqxmd.com/read/27909078/a-nanoscale-view-of-junctophilin-2-and-ryanodine-receptors
#11
(no author information available yet)
No abstract text is available yet for this article.
December 1, 2016: Journal of Cell Science
https://www.readbyqxmd.com/read/27802169/junctophilin-2-in-the-nanoscale-organisation-and-functional-signalling-of-ryanodine-receptor-clusters-in-cardiomyocytes
#12
Michelle L Munro, Isuru D Jayasinghe, Qiongling Wang, Ann Quick, Wei Wang, David Baddeley, Xander H T Wehrens, Christian Soeller
Signalling nanodomains requiring close contact between the plasma membrane and internal compartments, known as 'junctions', are fast communication hubs within excitable cells such as neurones and muscle. Here, we have examined two transgenic murine models probing the role of junctophilin-2, a membrane-tethering protein crucial for the formation and molecular organisation of sub-microscopic junctions in ventricular muscle cells of the heart. Quantitative single-molecule localisation microscopy showed that junctions in animals producing above-normal levels of junctophilin-2 were enlarged, allowing the re-organisation of the primary functional protein within it, the ryanodine receptor (RyR; in this paper, we use RyR to refer to the myocardial isoform RyR2)...
December 1, 2016: Journal of Cell Science
https://www.readbyqxmd.com/read/27789286/effects-of-induced-na-ca-2-exchanger-overexpression-on-the-spatial-distribution-of-l-type-ca-2-channels-and-junctophilin-2-in-pressure-overloaded-hearts
#13
Yoshihiro Ujihara, Satoshi Mohri, Yuki Katanosaka
The Na(+)/Ca(2+) exchanger 1 (NCX1) is an essential Ca(2+) efflux system in cardiomyocytes. Although NCX1 is distributed throughout the sarcolemma, a subpopulation of NCX1 is localized to transverse (T)-tubules. There is growing evidence that T-tubule disorganization is a causal event that shifts the transition from hypertrophy to heart failure (HF). However, the detailed molecular mechanisms have not been clarified. Previously, we showed that induced NCX1 expression in pressure-overloaded hearts attenuates defective excitation-contraction coupling and HF progression...
November 25, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/27760414/junctophilin-2-gene-therapy-rescues-heart-failure-by-normalizing-ryr2-mediated-ca-2-release
#14
Julia O Reynolds, Ann P Quick, Qiongling Wang, David L Beavers, Leonne E Philippen, Jordan Showell, Giselle Barreto-Torres, Donna J Thuerauf, Shirin Doroudgar, Christopher C Glembotski, Xander H T Wehrens
BACKGROUND: Junctophilin-2 (JPH2) is the primary structural protein for the coupling of transverse (T)-tubule associated cardiac L-type Ca channels and type-2 ryanodine receptors on the sarcoplasmic reticulum within junctional membrane complexes (JMCs) in cardiomyocytes. Effective signaling between these channels ensures adequate Ca-induced Ca release required for normal cardiac contractility. Disruption of JMC subcellular domains, a common feature of failing hearts, has been attributed to JPH2 downregulation...
December 15, 2016: International Journal of Cardiology
https://www.readbyqxmd.com/read/27749395/pathogenic-insights-from-huntington-s-disease-like-2-and-other-huntington-s-disease-genocopies
#15
Russell L Margolis, Dobrila D Rudnicki
PURPOSE OF REVIEW: Huntington's disease-like 2 (HDL2) is a rare, progressive, autosomal dominant neurodegenerative disorder that genetically, clinically, and pathologically closely resembles Huntington's disease. We review HDL2 pathogenic mechanisms and examine the implications of these mechanisms for Huntington's disease and related diseases. RECENT FINDINGS: HDL2 is caused by a CTG/CAG repeat expansion in junctophilin-3. Available data from cell and animal models and human brain suggest that HDL2 is a complex disease in which transcripts and proteins expressed bidirectionally from the junctophilin-3 locus contribute to pathogenesis through both gain-and loss-of-function mechanisms...
December 2016: Current Opinion in Neurology
https://www.readbyqxmd.com/read/27729468/speg-striated-muscle-preferentially-expressed-protein-kinase-is-essential-for-cardiac-function-by-regulating-junctional-membrane-complex-activity
#16
Ann P Quick, Qiongling Wang, Leonne E Philippen, Giselle Barreto-Torres, David Y Chiang, David Beavers, Guoliang Wang, Maha Khalid, Julia O Reynolds, Hannah M Campbell, Jordan Showell, Mark D McCauley, Arjen Scholten, Xander H T Wehrens
RATIONALE: Junctional membrane complexes (JMCs) in myocytes are critical microdomains, in which excitation-contraction coupling occurs. Structural and functional disruption of JMCs underlies contractile dysfunction in failing hearts. However, the role of newly identified JMC protein SPEG (striated muscle preferentially expressed protein kinase) remains unclear. OBJECTIVE: To determine the role of SPEG in healthy and failing adult hearts. METHODS AND RESULTS: Proteomic analysis of immunoprecipitated JMC proteins ryanodine receptor type 2 and junctophilin-2 (JPH2) followed by mass spectrometry identified the serine-threonine kinase SPEG as the only novel binding partner for both proteins...
January 6, 2017: Circulation Research
https://www.readbyqxmd.com/read/27630576/morn5-expression-during-craniofacial-development-and-its-interaction-with-the-bmp-and-tgf%C3%AE-pathways
#17
Petra Cela, Marek Hampl, Katherine K Fu, Michaela Kunova Bosakova, Pavel Krejci, Joy M Richman, Marcela Buchtova
MORN5 (MORN repeat containing 5) is encoded by a locus positioned on chromosome 17 in the chicken genome. The MORN motif is found in multiple copies in several proteins including junctophilins or phosphatidylinositol phosphate kinase family and the MORN proteins themselves are found across the animal and plant kingdoms. MORN5 protein has a characteristic punctate pattern in the cytoplasm in immunofluorescence imaging. Previously, MORN5 was found among differentially expressed genes in a microarray profiling experiment of the chicken embryo head...
2016: Frontiers in Physiology
https://www.readbyqxmd.com/read/27523632/disruption-of-calpain-reduces-lipotoxicity-induced-cardiac-injury-by-preventing-endoplasmic-reticulum-stress
#18
Shengcun Li, Lulu Zhang, Rui Ni, Ting Cao, Dong Zheng, Sidong Xiong, Peter A Greer, Guo-Chang Fan, Tianqing Peng
Diabetes and obesity are prevalent in westernized countries. In both conditions, excessive fatty acid uptake by cardiomyocytes induces cardiac lipotoxicity, an important mechanism contributing to diabetic cardiomyopathy. This study investigated the effect of calpain disruption on cardiac lipotoxicity. Cardiac-specific capns1 knockout mice and their wild-type littermates (male, age of 4weeks) were fed a high fat diet (HFD) or normal diet for 20weeks. HFD increased body weight, altered blood lipid profiles and impaired glucose tolerance comparably in both capns1 knockout mice and their wild-type littermates...
November 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27336719/junctophilin-3-expresses-in-pancreatic-beta-cells-and-is-required-for-glucose-stimulated-insulin-secretion
#19
L Li, Z-F Pan, X Huang, B-W Wu, T Li, M-X Kang, R-S Ge, X-Y Hu, Y-H Zhang, L-J Ge, D-Y Zhu, Y-L Wu, Y-J Lou
It is well accepted that junctophilin (JPHs) isoforms act as a physical bridge linking plasma membrane and endoplasmic reticulum (ER) for channel crosstalk in excitable cells. Our purpose is to investigate whether JPHs are involved in the proper communication between Ca(2+) influx and subsequent Ca(2+) amplification in pancreatic beta cells, thereby participating in regulating insulin secretion. The expression of JPH isoforms was examined in human and mouse pancreatic tissues, and JPH3 expression was found in both the beta cells...
June 23, 2016: Cell Death & Disease
https://www.readbyqxmd.com/read/27226008/elevated-ventricular-wall-stress-disrupts-cardiomyocyte-t-tubule-structure-and-calcium-homeostasis
#20
Michael Frisk, Marianne Ruud, Emil K S Espe, Jan Magnus Aronsen, Åsmund T Røe, Lili Zhang, Per Andreas Norseng, Ole M Sejersted, Geir A Christensen, Ivar Sjaastad, William E Louch
AIMS: Invaginations of the cellular membrane called t-tubules are essential for maintaining efficient excitation-contraction coupling in ventricular cardiomyocytes. Disruption of t-tubule structure during heart failure has been linked to dyssynchronous, slowed Ca(2+) release and reduced power of the heartbeat. The underlying mechanism is, however, unknown. We presently investigated whether elevated ventricular wall stress triggers remodelling of t-tubule structure and function. METHODS AND RESULTS: MRI and blood pressure measurements were employed to examine regional wall stress across the left ventricle of sham-operated and failing, post-infarction rat hearts...
October 2016: Cardiovascular Research
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