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Zhihua Hua, Paymon Doroodian, William Vu
Ubiquitin (Ub) and Ub-like proteins, collectively forming the ubiquiton family, regulate nearly all aspects of cellular processes via posttranslational modifications. Studies devoted to specific members suggested a large expansion of this family in plants. However, lack of its systematic analysis hinders the comparison of individual members at both evolutionary history and functional divergence levels, which may provide new insight into biological functions. In this work, we first retrieved in total 5,856 members of 17 known ubiquiton subfamilies in 50 plant genomes by searching both prior annotations and missing loci in each genome...
May 8, 2018: Plant Journal: for Cell and Molecular Biology
Ai-Min Wang, Shi-Yan Chen, Ge-Zhi Shen, Xin-Qi Wang, Dan-Hua Ju, Zhong-Lin Wang, Zong-Yang Wang, Xiu-Ling Cai
The coding region of Bar gene, the left border of Ds element, the coding region of GUS gene, the transposase of Ac element, the right border of Ds element and the promoter of Ubi gene were inserted into the T-DNA region of vector pCAMBIA1300 in turn to construct plasmid p13B. The orientations of the ubiquitons' promoter, Ac transposase and Bar are identical but opposite to that of the GUS gene (Fig.1). The plasmid p13B was then introduced into the calli of Oryza sativa subsp. japonica cv. Zhonghua 11 by Agrobacterium tumefaciens-mediated transforming to trap genes in rice...
December 2005: Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao, Journal of Plant Physiology and Molecular Biology
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