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https://www.readbyqxmd.com/read/27916606/fbx8-is-a-metastasis-suppressor-downstream-of-mir-223-and-targeting-mtor-for-degradation-in-colorectal-cancer
#1
F F Wang, X J Zhang, Y R Yan, X H Zhu, J Yu, Y Ding, J L Hu, W J Zhou, Z C Zeng, W T Liao, Y Q Ding, L Liang
F-box proteins are critical components of the SKP1-CUL1-F-box (SCF) E3 ubiquitin ligases and involved in the ubiquitin-dependent proteolytic pathway. Dysregulation of F-box protein-mediated proteolysis often leads to human malignancies. F-box only protein 8 (FBX8), a novel component of F-box proteins, is down-regulated in several tumors and closely correlates with tumor progression. However, little is known about its function, regulatory mechanisms and substrates in the progression of colorectal cancer (CRC)...
December 1, 2016: Cancer Letters
https://www.readbyqxmd.com/read/27915416/opposing-effects-on-the-cell-cycle-of-t-lymphocytes-by-fbxo7-via-cdk6-and-p27
#2
Shachi P Patel, Suzanne J Randle, Sarah Gibbs, Anne Cooke, Heike Laman
G1 phase cell cycle proteins, such as cyclin-dependent kinase 6 (Cdk6) and its activating partners, the D-type cyclins, are important regulators of T-cell development and function. An F-box protein, called F-box only protein 7 (Fbxo7), acts as a cell cycle regulator by enhancing cyclin D-Cdk6 complex formation and stabilising levels of p27, a cyclin-dependent kinase inhibitor. We generated a murine model of reduced Fbxo7 expression to test its physiological role in multiple tissues and found that these mice displayed a pronounced thymic hypoplasia...
December 3, 2016: Cellular and Molecular Life Sciences: CMLS
https://www.readbyqxmd.com/read/27909441/apple-f-box-protein-mdmax2-regulates-plant-photomorphogenesis-and-stress-response
#3
Jian-Ping An, Rui Li, Feng-Jia Qu, Chun-Xiang You, Xiao-Fei Wang, Yu-Jin Hao
MAX2 (MORE AXILLARY GROWTH2) is involved in diverse physiological processes, including photomorphogenesis, the abiotic stress response, as well as karrikin and strigolactone signaling-mediated shoot branching. In this study, MdMAX2, an F-box protein that is a homolog of Arabidopsis MAX2, was identified and characterized. Overexpression of MdMAX2 in apple calli enhanced the accumulation of anthocyanin. Ectopic expression of MdMAX2 in Arabidopsis exhibited photomorphogenesis phenotypes, including increased anthocyanin content and decreased hypocotyl length...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27900537/rnai-mediated-resistance-to-rice-black-streaked-dwarf-virus-in-transgenic-rice
#4
Mohamed M S Ahmed, Shiquan Bian, Muyue Wang, Jing Zhao, Bingwei Zhang, Qiaoquan Liu, Changquan Zhang, Shuzhu Tang, Minghong Gu, Hengxiu Yu
Rice black-streaked dwarf virus (RBSDV), a member of the genus Fijivirus in the family Reoviridae, causes significant economic losses in rice production in China and many other Asian countries. Development of resistant varieties by using conventional breeding methods is limited, as germplasm with high level of resistance to RBSDV have not yet been found. One of the most promising methods to confer resistance against RBSDV is the use of RNA interference (RNAi) technology. RBSDV non-structural protein P7-2, encoded by S7-2 gene, is a potential F-box protein and involved in the plant-virus interaction through the ubiquitination pathway...
November 30, 2016: Transgenic Research
https://www.readbyqxmd.com/read/27900032/mir-223-decreases-cell-proliferation-and-enhances-cell-apoptosis-in-acute-myeloid-leukemia-via-targeting-fbxw7
#5
Yi Xiao, Changliang Su, Taoran Deng
The expression of microRNA-223 (miR-233) has been investigated in various types of cancer. However, to the best of our knowledge, the expression and function of miR-223 in acute myeloid leukemia (AML) remains to be elucidated. The expression of miR-223 was measured by reverse transcription-quantitative polymerase chain reaction. Following transfection with miR-223, cell viability assays, cell apoptosis assays, western blot analysis and luciferase assays were conducted in AML cell lines. In the present study, it was initially observed that miR-223 was downregulated in AML patients compared with healthy subjects...
November 2016: Oncology Letters
https://www.readbyqxmd.com/read/27898393/a-noncanonical-auxin-sensing-mechanism-is-required-for-organ-morphogenesis-in-arabidopsis
#6
Sara Simonini, Joyita Deb, Laila Moubayidin, Pauline Stephenson, Manoj Valluru, Alejandra Freire-Rios, Karim Sorefan, Dolf Weijers, Jiří Friml, Lars Østergaard
Tissue patterning in multicellular organisms is the output of precise spatio-temporal regulation of gene expression coupled with changes in hormone dynamics. In plants, the hormone auxin regulates growth and development at every stage of a plant's life cycle. Auxin signaling occurs through binding of the auxin molecule to a TIR1/AFB F-box ubiquitin ligase, allowing interaction with Aux/IAA transcriptional repressor proteins. These are subsequently ubiquitinated and degraded via the 26S proteasome, leading to derepression of auxin response factors (ARFs)...
October 15, 2016: Genes & Development
https://www.readbyqxmd.com/read/27895653/genome-wide-identification-and-expression-analysis-of-the-tubby-like-protein-family-in-the-malus-domestica-genome
#7
Jia-Ning Xu, Shan-Shan Xing, Zheng-Rong Zhang, Xue-Sen Chen, Xiao-Yun Wang
Tubby-like proteins (TLPs), which have a highly conserved β barrel tubby domain, have been found to be associated with some animal-specific characteristics. In the plant kingdom, more than 10 TLP family members were identified in Arabidopsis, rice and maize, and they were found to be involved in responses to stress. The publication of the apple genome makes it feasible to systematically study the TLP family in apple. In this investigation, nine TLP encoding genes (TLPs for short) were identified. When combined with the TLPs from other plant species, the TLPs were divided into three groups (group A, B, and C)...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27890708/fbxl18-targets-lrrk2-for-proteasomal-degradation-and-attenuates-cell-toxicity
#8
Xiaodong Ding, Sandeep K Barodia, Lisha Ma, Matthew S Goldberg
Dominantly inherited mutations in leucine-rich repeat kinase 2 (LRRK2) are the most common cause of familial Parkinson's disease (PD) and LRRK2 polymorphisms are associated with increased risk for idiopathic PD. However, the molecular mechanisms by which these mutations cause PD remain uncertain. In vitro studies indicate that disease-linked mutations in LRRK2 increase LRRK2 kinase activity and LRRK2-mediated cell toxicity. Identifying LRRK2-interacting proteins and determining their effects on LRRK2 are important for understanding LRRK2 function and for delineating the pathophysiological mechanisms of LRRK2 mutations...
November 24, 2016: Neurobiology of Disease
https://www.readbyqxmd.com/read/27889482/the-red-light-receptor-phytochrome-b-directly-enhances-substrate-e3-ligase-interactions-to-attenuate-ethylene-responses
#9
Hui Shi, Xing Shen, Renlu Liu, Chang Xue, Ning Wei, Xing Wang Deng, Shangwei Zhong
Plants germinating under subterranean darkness assume skotomorphogenesis, a developmental program strengthened by ethylene in response to mechanical pressure of soil. Upon reaching the surface, light triggers a dramatic developmental transition termed de-etiolation that requires immediate termination of ethylene responses. Here, we report that light activation of photoreceptor phyB results in rapid degradation of EIN3, the master transcription factor in the ethylene signaling pathway. As a result, light rapidly and efficiently represses ethylene actions...
November 16, 2016: Developmental Cell
https://www.readbyqxmd.com/read/27881728/dii-based-auxin-reporter-in-maize-reveals-low-auxin-signaling-during-telophase-and-early-g1
#10
Ricardo Mir, Leslie Z Aranda, Tiffany Baiocchi, Anding Luo, Anne Sylvester, Carolyn Rasmussen
A sensitive and dynamically responsive auxin signaling reporter based on the DII domain of the INDOLE-3-ACETIC ACID28 (IAA28, DII) protein from Arabidopsis thaliana was modified for use in maize. The DII domain was fused to a yellow fluorescent protein and a nuclear localization sequence to simplify quantitative nuclear fluorescence signal. DII degradation dynamics provide an estimate of input signal into the auxin signaling pathway that is influenced by both auxin accumulation and F-box co-receptor concentration...
November 23, 2016: Plant Physiology
https://www.readbyqxmd.com/read/27880725/mouse-sirt3-promotes-autophagy-in-angii-induced-myocardial-hypertrophy-through-the-deacetylation-of-foxo1
#11
Jingyuan Li, Tongshuai Chen, Ming Xiao, Na Li, Shujian Wang, Hongyan Su, Xiaobin Guo, Hui Liu, Fangying Yan, Yi Yang, Yun Zhang, Peili Bu
Sirt3, a mitochondrial NAD+-dependent histone deacetylase, is the only member proven to promote longevity in mammalian Sirtuin family. The processed short form of Sirt3 has been demonstrated to target many mediators of energy metabolism and mitochondrial stress adaptive program. Autophagy serves as a dynamic recycling mechanism and provides energy or metabolic substrates. Among the mechanisms triggered by cardiac stress, opinions vary as to whether autophagy is a protective or detrimental response. Here, by inducing the Sirt3-knockout mice to myocardial hypertrophy with chronic angiotensin II infusion for four weeks, we determined the role of Sirt3 in myocardial hypertrophy and autophagy...
November 17, 2016: Oncotarget
https://www.readbyqxmd.com/read/27877177/comparative-transcriptomic-analyses-of-differentially-expressed-genes-in-transgenic-melatonin-biosynthesis-ovine-hiomt-gene-in-switchgrass
#12
Shan Yuan, Cong Guan, Sijia Liu, Yanhua Huang, Danyang Tian, Xin Cui, Yunwei Zhang, Fuyu Yang
Melatonin serves pleiotropic functions in prompting plant growth and resistance to various stresses. The accurate biosynthetic pathway of melatonin remains elusive in plant species, while the N-acetyltransferase and O-methyltransferase were considered to be the last two key enzymes during its biosynthesis. To investigate the biosynthesis and metabolic pathway of melatonin in plants, the RNA-seq profile of overexpression of the ovine HIOMT was analyzed and compared with the previous transcriptome of transgenic oAANAT gene in switchgrass, a model plant for cellulosic ethanol production...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27864766/assaying-auxin-receptor-activity-using-spr-assays-with-f-box-proteins-and-aux-iaa-degrons
#13
Mussa Quareshy, Veselina Uzunova, Justyna M Prusinska, Richard M Napier
The identification of TIR1 as an auxin receptor combined with advanced biophysical instrumentation has led to the development of real-time activity assays for auxins. Traditionally, molecules have been assessed for auxinic activity using bioassays, and agrochemical compound discovery continues to be based on "spray and pray" technologies. Here, we describe the methodology behind an SPR-based assay that uses TIR1 and related F-box proteins with surface plasmon resonance spectrometry for rapid compound screening...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27861377/familial-atypical-parkinsonism-with-rare-variant-in-vps35-and-fbxo7-genes-a-case-report
#14
Tereza Bartonikova, Katerina Mensikova, Lenka Mikulicova, Radek Vodicka, Radek Vrtel, Marek Godava, Miroslav Vastik, Michaela Kaiserova, Pavel Otruba, Iva Dolinova, Martin Nevrly, Petr Kanovsky
BACKGROUND: A higher prevalence of parkinsonism was recently identified in southeastern Moravia (Czech Republic). Further research confirmed 3 large pedigrees with familial autosomal-dominant parkinsonism spanning 5 generations. METHODS: This case report concerns a patient belonging to one of these 3 pedigrees, in whom motor and oculomotor symptoms were accompanied by frontal-type dementia, who finally developed a clinical phenotype of progressive supranuclear palsy...
November 2016: Medicine (Baltimore)
https://www.readbyqxmd.com/read/27856247/the-drosophila-f-box-protein-slimb-controls-dsmurf-protein-turnover-to-regulate-the-hippo-pathway
#15
Liangchang Hu, Ping Wang, Runan Zhao, Shanshan Li, Feng Wang, Chaojie Li, Lei Cao, Shian Wu
SMAD ubiquitination regulatory factors 1 and 2 (Smurf1/2) are members of the HECT domain E3 ligase family which play crucial roles in the regulation of cell cycle progression, planar cell polarity, cancer metastasis and cell apoptosis. We recently showed that the Drosophila homolog dSmurf controls the stability of Warts kinase to regulate the Hippo pathway. In the current study, we found that the F-box protein Slimb controls dSmurf protein level to regulate the Hippo pathway. Slimb physically associates with dSmurf as revealed by co-immunoprecipitation assay in S2 cells...
November 14, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/27855403/fbxo6-mediated-ubiquitination-and-degradation-of-ero1l-inhibits-endoplasmic-reticulum-stress-induced-apoptosis
#16
Xi Chen, Lang-Huan Duan, Peng-Cheng Luo, Gang Hu, Xin Yu, Jie Liu, Han Lu, Bin Liu
BACKGROUND/AIMS: FBXO6 is the substrate recognition component of a Skp1-Cullin1-F-box protein (SCF) ubiquitin E3 ligase complex, recognizing the chitobiose in unfolded N-glycoprotein to target glycoproteins for polyubiquitination and degradation. Although how FBXO6 recognizes glycoprotein has been fully investigated, the ubiquitination substrates of FBXO6 remain largely unknown. Previously, we have systematically identified the glycoproteins that interact with FBXO6 in an N-glycan dependent manner by LC/MS spectrum and confirmed the interaction between FBXO6 and glycosylated Ero1L, a protein disulfide oxidase in endoplasmic reticulum (ER)...
2016: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/27854312/berberine-suppresses-cyclin-d1-expression-through-proteasomal-degradation-in-human-hepatoma-cells
#17
Ning Wang, Xuanbin Wang, Hor-Yue Tan, Sha Li, Chi Man Tsang, Sai-Wah Tsao, Yibin Feng
The aim of this study is to explore the underlying mechanism on berberine-induced Cyclin D1 degradation in human hepatic carcinoma. We observed that berberine could suppress both in vitro and in vivo expression of Cyclin D1 in hepatoma cells. Berberine exhibits dose- and time-dependent inhibition on Cyclin D1 expression in human hepatoma cell HepG2. Berberine increases the phosphorylation of Cyclin D1 at Thr286 site and potentiates Cyclin D1 nuclear export to cytoplasm for proteasomal degradation. In addition, berberine recruits the Skp, Cullin, F-box containing complex-β-Transducin Repeat Containing Protein (SCF(β-TrCP)) complex to facilitate Cyclin D1 ubiquitin-proteasome dependent proteolysis...
November 15, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27846808/pinpointing-genes-underlying-annual-perennial-transitions-with-comparative-genomics
#18
Andrew J Heidel, Christiane Kiefer, George Coupland, Laura E Rose
BACKGROUND: Transitions between perennial and an annual life history occur often in plant lineages, but the genes that control whether a plant is an annual or perennial are largely unknown. To identify genes that confer differences between annuals and perennials we compared the gene content of four pairs of sister lineages (Arabidopsis thaliana/Arabidopsis lyrata, Arabis montbretiana/Arabis alpina, Arabis verna/Aubrieta parviflora and Draba nemorosa/Draba hispanica) in the Brassicaceae in which each pair contains one annual and one perennial, plus one extra annual species (Capsella rubella)...
November 15, 2016: BMC Genomics
https://www.readbyqxmd.com/read/27844101/functional-identification-of-apple-mdjaz2-in-arabidopsis-with-reduced-ja-sensitivity-and-increased-stress-tolerance
#19
Xiu-Hong An, Yu-Jin Hao, En-Mao Li, Kai Xu, Cun-Gang Cheng
Here, we report the decrease of JA-sensitivity and enhancement of tolerance to salt and PEG stresses in Arabidopsis overexpressing apple MdJAZ2. As signalling molecules, jasmonates (JAs) play significant roles in plant development and stress responses. JAZ proteins are the targets of the SCF(COI1) complex and act as the negative regulators in JA signalling pathway. However, there are no reports regarding the biological function of apple JAZ genes. In this study, one JAZ gene, MdJAZ2 from apple, was functionally characterized in detail...
November 14, 2016: Plant Cell Reports
https://www.readbyqxmd.com/read/27835873/proteomic-identification-of-the-oncoprotein-stat3-as-a-target-of-a-novel-skp1-inhibitor
#20
Xin Cheng, Yong-Qiang Liu, Gui-Zhen Wang, Li-Na Yang, Yong-Zhi Lu, Xin-Chun Li, Bo Zhou, Li-Wei Qu, Xiao-Lu Wang, Yong-Xian Cheng, Jinsong Liu, Sheng-Ce Tao, Guang-Biao Zhou
The S phase kinase-associated protein 1 (Skp1), an adaptor protein of the Skp1-Cul1-F-box protein complex, binds the ubiquitin E3 ligase Skp2 and is critical to its biological functions. Targeting of Skp1 by a small compound 6-O-angeloylplenolin (6-OAP) results in dissociation and degradation of Skp2 and mitotic arrest of lung cancer cells. Here, by using a proteome microarray containing 16,368 proteins and a biotinylated 6-OAP, we identified 99 proteins that could bind 6-OAP, with Skp1 and STAT3 sitting at the central position of the 6-OAP interactome...
November 7, 2016: Oncotarget
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