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https://www.readbyqxmd.com/read/29910214/effect-of-a-single-epidural-administration-of-follicle-stimulating-hormone-via-caudal-vertebrae-on-superstimulation-for-in-vivo-and-in-vitro-embryo-production-in-japanese-black-cows
#1
Kenichiro Sakaguchi, Atsushi Ideta, Yojiro Yanagawa, Masashi Nagano, Seiji Katagiri, Masato Konishi
Here, we describe a simplified procedure for embryo production in the Japanese black cow that uses a single caudal epidural injection of follicle-stimulating hormone (FSH). First, we compared the efficiency of superovulation for in vivo embryo production between conventional multiple FSH treatment (control, n = 10) and single epidural administration (epidural, n = 5). The number of transferable blastocysts was similar between control and epidural groups (4.7 ± 3.5 and 9.0 ± 6.0, respectively). Next, we compared in vitro embryo production by ovum pick-up and in vitro fertilization (OPU-IVF) between control (n = 12) and epidural groups (n = 12)...
June 16, 2018: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/29907333/superstimulation-with-ecg-prior-to-ovum-pick-up-improves-follicular-development-and-fertilization-rate-of-cattle-oocytes
#2
Bibiana Noal Ribas, Daniele Missio, Isac Junior Roman, Normélio Alves Neto, Izaias Claro, Daniela Dos Santos Brum, Fabio Gallas Leivas
This study aimed to evaluate the effect of equine chorionic gonadotrophin (eCG) stimulation prior to ovum pick-up (OPU) on follicular development, number and quality of recovered oocytes, fertilization rate, and early embryo development in vitro. There were four OPU sessions (cross over) conducted on 16 Braford cows to evaluate the effect of various eCG doses. The timing of the wave of ovarian follicular development was synchronized, and three days after, the respective eCG dose was administrated (0, 200, 400, or 800 IU)...
June 9, 2018: Animal Reproduction Science
https://www.readbyqxmd.com/read/29906662/hormonal-stimulation-in-4-to-7-months-old-nelore-bos-taurus-indicus-females-improved-ovarian-follicular-responses-but-not-the-in-vitro-embryo-production
#3
Thaís Abritta Zacarias, Taynan Stonoga Kawamoto, João Ricardo Scaliante Júnior, Ana Luiza Silva Guimarães, Maurício Machaim Franco, Ricardo Alamino Figueiredo
The inclusion of pre-pubertal bovine females in reproductive management could allow in vitro embryo production and reduce generation interval, thereby causing faster genetic gain of the herd. However, oocytes of pre-pubertal females have lower competence, blastocyst production, and pregnancy rates than those collected from pubertal animals. This study aimed to evaluate the effect of an induced hormonal stimulation on the serum concentrations of Anti-Mullerian hormone (AMH) and FSH, ovarian responses, ovum pick up (OPU), and in vitro produced embryos (IVP) from oocytes obtained from four-to seven-months old Nelore female cattle...
May 31, 2018: Theriogenology
https://www.readbyqxmd.com/read/29890350/complicated-clinical-course-and-poor-reproductive-outcomes-of-women-with-tuboovarian-abscess-following-fertility-treatments
#4
Yuval Fouks, Yoni Cohen, Togas Tulandi, Amir Meiri, Ishai Levin, Benny Almog, Aviad Cohen
OBJECTIVE: To assess the clinical course, surgical and fertility outcomes of patients diagnosed with tuboovarian abscess (TOA) following fertility treatment. DESIGN: Parallel case series over 10 consecutive years. DESIGN CLASSIFICATION: Canadian Task Force classification II-2. SETTING: Tel Aviv Sourasky Medical Center, a tertiary university-affiliated hospital. PATIENTS: Thirty-seven women who were diagnosed with TOA following fertility treatments (in-vitro fertilization and intrauterine insemination) were compared with 313 women who were diagnosed with TOA not associated with fertility treatments during the same time period...
June 8, 2018: Journal of Minimally Invasive Gynecology
https://www.readbyqxmd.com/read/29877232/-ovum-collection-following-ovarian-stimulation-in-patients-with-chronic-active-epstein-barr-virus-infection
#5
Erika Onozawa, Tomonori Ishikawa, Yasuhiro Tomaru, Norio Shimizu, Ayako Arai
The only curative treatment for chronic active Epstein-Barr virus infection (CAEBV) is hematopoietic stem cell transplantation. For young female patients, ovulation induction and oocyte cryopreservation may be performed prior to transplantation to provide for future pregnancies. However, the effects of this ovum treatment on CAEBV and EBV infections have not been reported. Attempts were made to collect ova from three female CAEBV patients before transplantation conditioning, but this was only successful in two cases...
2018: [Rinshō Ketsueki] the Japanese Journal of Clinical Hematology
https://www.readbyqxmd.com/read/29778922/interval-of-gonadotropin-administration-for-in-vitro-embryo-production-from-oocytes-collected-from-holstein-calves-between-2-and-6-months-of-age-by-repeated-laparoscopy
#6
Hernan Baldassarre, Luke Currin, Laura Michalovic, Anne-Marie Bellefleur, Karina Gutierrez, Rafael G Mondadori, Werner G Glanzner, Yasmin Schuermann, Rodrigo C Bohrer, Naomi Dicks, Rosalba Lopez, François-Xavier Grand, Christian Vigneault, Patrick Blondin, Jim Gourdon, Vilceu Bordignon
Laparoscopic Ovum Pick-Up (LOPU) in calves followed by in vitro embryo production (IVEP) and transfer (ET) into adult recipients has great potential for accelerated genetic gain through shortening of the generation interval. In this study, 11 Holstein calves were subjected to up to six LOPU procedures between the ages of 2-6 months at 2-3 weeks interval. In all cases, the animals received a CIDR 5 days prior to LOPU and were gonadotropin-stimulated starting at 72 h before LOPU using one of three protocols that were rotated twice among the animals during the study...
May 17, 2018: Theriogenology
https://www.readbyqxmd.com/read/29774968/granulysin-mediated-apoptosis-of-trophoblasts-in-blighted-ovum-and-missed-abortion
#7
Tamara Gulic, Gordana Laskarin, Marin Dominovic, Lana Glavan Gacanin, Emina Babarović, Zeljka Rubesa, Herman Haller, Daniel Rukavina
PROBLEM: Granulysin (GNLY) is a cytotoxic molecule mostly present in decidual natural killer (NK) cells. Blighted ovum (BO) and missed abortion (MA) represent the early pathological pregnancies with hindered development of the embryoblast or a dead embryo. We investigated the GNLY-mediated apoptotic mechanism potentially responsible for delayed termination of pregnancy. METHOD OF STUDY: We performed immunohistological and immunofluorescence labeling of decidual tissues (GNLY, Apaf-1, NF-κB)...
May 18, 2018: American Journal of Reproductive Immunology: AJRI
https://www.readbyqxmd.com/read/29753485/genome-wide-association-study-and-in-silico-functional-analysis-of-the-number-of-embryos-produced-by-holstein-donors
#8
C Jaton, F S Schenkel, M Sargolzaei, A Cánova, F Malchiodi, C A Price, C Baes, F Miglior
Superovulation or ovum pick-up and in vitro fertilization are technologies used to produce an increased number of embryos from elite females. Embryo production traits have been shown to be heritable, but the genes that cause this variability have not yet been assessed. The main objectives of this study were to perform a genome-wide association study (GWAS) to find single nucleotide polymorphisms (SNP) associated with embryo production traits and to identify candidate genes affecting the number of embryos produced by Holstein donors in Canada that may provide insight into the regulation of embryo production...
May 9, 2018: Journal of Dairy Science
https://www.readbyqxmd.com/read/29744644/an-epigenetic-association-of-malformations-adverse-reproductive-outcomes-and-fetal-origins
#9
Mark Lubinsky
VACTERL, the prototype for associated congenital anomalies, also has connections with functional issues such as pregnancy losses, prematurity, growth delays, perinatal difficulties, and parental subfertility. This segues into a broader association with similar connections even in the absence of malformations. DNA methylation disturbances in the ovum are a likely cause, with epigenetic links to individual components and to folate effects before conception, explaining diverse fetal and placental findings and providing a link to fetal origin hypothesis-related effects...
May 9, 2018: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/29739353/changes-in-serum-prolactin-level-during-intracytoplasmic-sperm-injection-and-effect-on-clinical-pregnancy-rate-a-prospective-observational-study
#10
Ahmed Kamel, Ayman A Halim, Mohamed Shehata, Salwa AlFarra, Yahia El-Faissal, Wafaa Ramadan, Ahmed M Hussein
BACKGROUND: Transient hyperprolactinemia was proven to adversely affect the outcome of IVF. We aimed to identify changes in serum prolactin levels in patients undergoing ICSI, and to evaluate the effect of these changes on the clinical pregnancy rate. METHODS: A prospective observational study included 90 patients scheduled for ICSI cycles. In each case 4 serum samples were collected during the cycle (midluteal, before ovum pick up procedure (OPU), 2 h after OPU, and before embryo transfer)...
May 9, 2018: BMC Pregnancy and Childbirth
https://www.readbyqxmd.com/read/29699383/human-embryonic-stem-cell-derived-from-early-stage-fertilized-ovum-non-immunogenic-and-universal-neuronal-and-non-neuronal-cell-lines
#11
Geeta Shroff, Arpita Srivastav, Rohan Shroff
Background: Human embryonic stem cells (hESCs) have the potential to treat various human disorders currently labeled as incurable and/or terminal illness. However, the fear that the patients' immune system would recognize them as non self and lead to an immune rejection has hampered their use. The main cause for immune rejection is usually the incompatibility of both donor and recipient's major histocompatibility complex (MHC). Methods: We describe a hESC line developed through a patented technology that does not lead to immune reaction upon transplantation...
May 30, 2018: International Journal of Stem Cells
https://www.readbyqxmd.com/read/29696351/the-effects-of-superovulation-with-gonadotropins-on-autoantibody-levels-in-patients-undergoing-assisted-reproductive-cycles
#12
Mahnaz Ashrafi, Elham Amirchaghmaghi, Arezoo Arabipoor, Samira Vesali, Reza Salman-Yazdi
PURPOSE: To evaluate the effect of controlled ovarian stimulation (COH) with gonadotropins on the serum levels of autoantibodies in the women who underwent in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) cycles and to compare basal levels of these autoantibodies between groups according to history of COH. METHODS: This prospective cohort study was performed from October 2014 to March 2016 in the Royan Institute. The volunteered infertile women with regard to the inclusion criteria, who underwent IVF/ICSI cycles, were recruited...
July 2018: Archives of Gynecology and Obstetrics
https://www.readbyqxmd.com/read/29676088/-explore-microcosmic-connection-between-autophagy-mechanism-and-follicular-development-based-on-kidney-governing-reproduction-theory
#13
REVIEW
Jun Bai, Ke-Ming Wu, Ran-Ran Gao
In the theory of traditional Chinese medicine(TCM) that "kidney storing essence and governing reproduction", reproductive essence is an important part of the kidney essence and acts as the original material of offspring embryos. Sperm, oocyte and zygote should be all included in the range of reproductive essence. Ovum is the essence of reproduction from inborn. The follicles maturation depends on the quality of oocyte and the vigor of kidney essence. Meanwhile, discharge of mature ovum relies on the stimulation and promotion by kidney Qi...
March 2018: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29650046/blighted-ovum-and-tubal-pregnancy-a-rare-form-of-heterotopic-pregnancy-case-report
#14
Desmond Aroke, Larry Tangie Ngek, Maxime Tindong, Esua Fomanka, Colette Achu, Arnold Agwe Tanah, Benjamin Momo Kadia
BACKGROUND: Heterotopic pregnancies are rare in spontaneous conceptions. Nonetheless, when it does occur, the intrauterine pregnancy is usually viable. We herein present a true rarity of the coexistence of a blighted ovum and an ectopic pregnancy. CASE PRESENTATION: A 25 year old G2P1001 married seamstress of African ethnicity at 8 weeks of amenorrhoea presented to our health facility with a 4 day history of lower abdominal pains and vaginal bleeding for which physical examination revealed a closed cervix...
April 12, 2018: BMC Research Notes
https://www.readbyqxmd.com/read/29549827/variations-in-bovine-embryo-production-between-individual-donors-for-opu-ivf-are-closely-related-to-glutathione-concentrations-in-oocytes-during-in-vitro-maturation
#15
Takemasa Hidaka, Yutaka Fukumoto, Satoshi Yamamoto, Yasuhiro Ogata, Toshitaka Horiuchi
Variations in embryo production between individual oocyte donors represent a serious problem in cattle production, when implementing ovum pick-up (OPU) and in vitro maturation (IVM) of oocytes. However, the precise cause of this problem is unknown. Here, we aimed to investigate the relationship between the glutathione (GSH) concentration in IVM oocytes and embryo development to explore a potential cause of the variations between individual donors. First, we found a high positive correlation (r = 0.80) between the GSH concentration in IVM oocytes and the blastocyst development rate of oocytes collected in the same OPU session for each donor (N = 11)...
June 2018: Theriogenology
https://www.readbyqxmd.com/read/29453138/increased-coagulation-index-as-measured-by-thromboelastography-during-ovarian-stimulation-for-in-vitro-fertilization-influence-of-the-final-oocyte-maturation-triggering-agent
#16
Ronit Beck-Fruchter, Israel Gavish, Shira Baram, Yoel Geslevich, Amir Weiss
OBJECTIVE: Thromboelastography (TEG) is a viscoelastic test of hemostasis which allows measurement of the processes of clot initiation, propagation, stabilization, and dissolution in real time. In this study we aimed to evaluate the alterations in coagulation as measured by TEG during In Vitro Fertilization (IVF) stimulation cycles and to investigate whether final oocyte maturation with recombinant hCG (rhCG) versus GnRH agonist results in a different coagulation state. STUDY DESIGN: This is a prospective observational study which included fifty-three normogonadotrophic women...
April 2018: European Journal of Obstetrics, Gynecology, and Reproductive Biology
https://www.readbyqxmd.com/read/29435117/replication-of-hepatitis-e-virus-in-the-ovary-and-promotion-of-oocyte-apoptosis-in-rabbits-infected-with-hev-4
#17
Junqing An, Tianlong Liu, Ruiping She, Qiaoxing Wu, Jijing Tian, Ruihan Shi, Wenzhuo Hao, Xinxin Ren, Yue Yang, Yiyao Lu, Yifei Yang, Yuanheng Wu
Hepatitis E virus (HEV) infection can induce infertility and miscarriage in pregnant women and infect neonates through vertical transmission. However, the mechanism of infertility and vertical transmission remains unclear. In the present study, we evaluated the replication of HEV in the ovary and structural and molecular changes induced by HEV after intraperitoneal injection of HEV in rabbits. Positive- and negative-strand HEV RNA was detected in the ovaries at 28 and 49 days post-infection. Positive HEV open reading frames 2 and 3 signals were observed in the ovaries by immunohistochemistry staining...
January 12, 2018: Oncotarget
https://www.readbyqxmd.com/read/29430150/on-the-origins-of-the-semen-analysis-a-close-relationship-with-the-history-of-the-reproductive-medicine
#18
REVIEW
Fernando Tadeu Andrade-Rocha
The spermatozoa were first seen in ejaculates in the 17th century. However, the basic mechanisms of human fertilization have been only fully understood after the discovery of ovum in 1827. As a result, the interest in developing technologies for semen analysis arose from the early 1900s. Indeed, standard methodologies for semen analysis were designed mostly along the first half of the 20th century. Before the 1930s, semen analysis was nearly unavailable clinically, since there were still no robust methodologies for assessing sperm characteristics, as well as to set up standard references that could be able to assess the reproductive capacity of men...
October 2017: Journal of Human Reproductive Sciences
https://www.readbyqxmd.com/read/29416828/asymmetrically-enlarged-parietal-foramina-in-a-rare-case-of-goldenhar-syndrome-with-a-possible-etiopathogenesis
#19
Lika'a Fasih Y Al-Kzayer, Shamil Naji Sarsam, Nagham Younus Alhur, Tingting Liu, Yozo Nakazawa
Goldenhar´s syndrome (GS) also known as oculo-auriculo-vertebral spectrum (OAVS) is a relatively rare condition. GS is of multifactorial etiology that includes environmental and/or genetic factors, in addition to teratogens that disturb the blastogenesis. A 5-year-old girl from Saudi Arabia, was a member of dizygotic twins conceived by assisted reproductive technology (ART), and born with features of GS. She had asymmetrical face, cleft lip and palate, right microphthalmia and microtia. Radiological imaging showed right maxillary and mandibular bone hypoplasia, asymmetrically enlarged parietal foramina, a persistent midline occipital foramen, abnormal bone arising from occipital bone, extending along tentorium cerebelli, and a lipoma at the right tentorium cerebelli...
January 5, 2018: Oncotarget
https://www.readbyqxmd.com/read/29383942/the-association-between-trans-fatty-acids-infertility-and-fetal-life-a-review
#20
Hande Çekici, Yasemin Akdevelioğlu
Trans fatty acids (TFAs) are thought to affect reproductive health by causing adverse effects on sperm morphology and ovum quality as a result of changing membrane lipid composition which, in turn, leads to impairment in metabolic pathways. This literature review examines the evidence for the effects of dietary TFAs on male and female infertility. Studies conducted between 2007 and 2017 on the effect of dietary TFAs on human reproductive health and fetal life have been included. They indicate that TFA intakes are inversely proportional to sperm concentration and total sperm count and exhibit a positive correlation with asthenospermia, as well as an adverse association on sperm concentration and semen quality...
January 31, 2018: Human Fertility: Journal of the British Fertility Society
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