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https://www.readbyqxmd.com/read/29040498/genome-stability-of-bovine-in-vivo-conceived-cleavage-stage-embryos-is-higher-compared-to-invitro-produced-embryos
#1
Olga Tšuiko, Maaike Catteeuw, Masoud Zamani Esteki, Aspasia Destouni, Osvaldo Bogado Pascottini, Urban Besenfelder, Vitezslav Havlicek, Katrien Smits, Ants Kurg, Andres Salumets, Thomas D'Hooghe, Thierry Voet, Ann Van Soom, Joris Robert Vermeesch
STUDY QUESTION: Is the rate and nature of chromosome instability (CIN) similar between bovine in vivo-derived and in vitro-cultured cleavage-stage embryos? SUMMARY ANSWER: There is a major difference regarding chromosome stability of in vivo-derived and in vitro-cultured embryos, as CIN is significantly lower in in vivo-derived cleavage-stage embryos compared to in vitro-cultured embryos. WHAT IS KNOWN ALREADY: CIN is common during in vitro embryogenesis and is associated with early embryonic loss in humans, but the stability of in vivo-conceived cleavage-stage embryos remains largely unknown...
September 23, 2017: Human Reproduction
https://www.readbyqxmd.com/read/29030199/effect-of-iodine-supplementation-in-pregnant-women-on-child-neurodevelopment-a-randomised-double-blind-placebo-controlled-trial
#2
Sueppong Gowachirapant, Nidhi Jaiswal, Alida Melse-Boonstra, Valeria Galetti, Sara Stinca, Ian Mackenzie, Susan Thomas, Tinku Thomas, Pattanee Winichagoon, Krishnamachari Srinivasan, Michael B Zimmermann
BACKGROUND: Iodine deficiency during pregnancy might be associated with reduced intelligence quotient (IQ) score in offspring. We assessed the effect of iodine supplementation in mildly iodine-deficient pregnant women on neurodevelopment of their offspring in areas where schoolchildren were iodine sufficient. METHODS: In this randomised, placebo-controlled trial, pregnant women in Bangalore, India, and Bangkok, Thailand, were randomly assigned (1:1) to receive 200 μg iodine orally once a day or placebo until delivery...
October 10, 2017: Lancet Diabetes & Endocrinology
https://www.readbyqxmd.com/read/29024760/rna-processing-in-the-male-germline-mechanisms-and-implications-for-fertility
#3
REVIEW
Julien M D Legrand, Robin M Hobbs
Mammalian spermatogenesis is a tightly coordinated process that gives rise to mature spermatozoa capable of fertilising an ovum during sexual reproduction. A population of stem and progenitor cells known as undifferentiated spermatogonia enables continual spermatogenesis throughout life. A complex transcriptional network that balances self-renewal of spermatogonia with their timely differentiation in order to maintain constant fertility regulates this process. Importantly, post-transcriptional regulation of gene expression plays a critical role in spermatogenesis, necessitated by the profound genetic and morphological changes that occur during meiosis and sperm maturation...
October 9, 2017: Seminars in Cell & Developmental Biology
https://www.readbyqxmd.com/read/28974845/challenges-in-the-routine-praxis-diagnosis-of-hydatidiform-mole-a-tertiary-health-center-experience
#4
Melisa Lelic, Zlatan Fatusic, Ermina Iljazovic, Suada Ramic, Sergije Markovic, Selma Alicelebic
INTRODUCTION: Hydatidiform moles (HM), presenting as complete (CHM) and partial (PHM) form, are rare pregnancy disorder. Diagnosis is based on clinical presentation, ultrasound imaging findings and pathological examination of products of conception. Protein p57, encoded by CKDN1C gene, is paternally imprinted and maternally expressed gene and provides quick insight in genetic basis of HM and allows distinction of CHM from all other conceptions. compare the preevacuational and pathohistological diagnosis with outcome of p57 immunostaining...
August 2017: Medical Archives
https://www.readbyqxmd.com/read/28960429/the-effects-of-low-dose-hcg-combined-with-hmg-protocol-on-women-with-hypogonadotropic-hypogonadism-undergoing-ovarian-stimulation-for-in-vitro-fertilization
#5
Shutian Jiang, Yanping Kuang
OBJECTIVES: To investigate the effects of low-dose human chorionic gonadotropin (hCG) combined with human menopausal gonadotropin (HMG) protocol on cycle characteristics and outcomes of infertile women with hypogonadotropic hypogonadism (HH) undergoing ovarian stimulation for in vitro fertilization (IVF). DESIGN: A retrospective cohort study. SETTING: Tertiary-care academic medical centre. PATIENT(S): Forty-six infertile patients with HH and seventy-one infertile patients with tubal factor (TF) infertility undergoing IVF...
September 27, 2017: Clinical Endocrinology
https://www.readbyqxmd.com/read/28940191/weekend-free-scheduled-ivf-icsi-procedures-and-single-embryo-transfer-do-not-reduce-live-birth-rates-in-a-general-infertile-population
#6
Michael Feichtinger, Per Olof Karlström, Jan I Olofsson, Kenny A Rodriguez-Wallberg
INTRODUCTION: Scheduling of ovum pickups (OPU) only on weekdays may result in cases of apparently suboptimal timing for human chorionic gonadotropin (hCG) and OPU. This study aimed to assess whether live-birth rates were reduced in women with a potentially suboptimal day for hCG and OPU to avoid week-end work, according to ultrasonographic data on the day of hCG planning. MATERIAL AND METHODS: An evaluation of the optimal hCG priming date was performed in treatment protocols of 1000 consecutive patients undergoing their first in vitro fertilization /intracytoplasmatic sperm injection (IVF/ICSI) with single-embryo transfer...
September 20, 2017: Acta Obstetricia et Gynecologica Scandinavica
https://www.readbyqxmd.com/read/28929977/exposure-to-follicular-fluid-during-oocyte-maturation-and-oviductal-fluid-during-post-maturation-does-not-improve-in-vitro-embryo-production-in-the-horse
#7
Cécile Douet, Olivia Parodi, Nicola Antonio Martino, Giovanni Michele Lacalandra, Michele Nicassio, Fabrice Reigner, Stefan Deleuze, Maria Elena Dell'Aquila, Ghylène Goudet
Most wild equids and many domestic horse breeds are at risk of extinction, so there is an urgent need for genome resource banking. Embryos cryopreservation allows the preservation of genetics from male and female and is the fastest method to restore a breed. In the equine, embryo production in vitro would allow the production of several embryos per cycle. Intracytoplasmic sperm injection (ICSI) is used to generate horse embryos, but it requires expensive equipment and expertise in micromanipulation, and blastocyst development rates remain low...
September 20, 2017: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/28911927/dynamic-expression-of-pgrmc1-and-serbp1-in-human-endometrium-an-implication-in-the-human-decidualization-process
#8
Stefania Salsano, Alicia Quiñonero, Silvia Pérez, Tamara Garrido Gómez, Carlos Simón, Francisco Dominguez
OBJECTIVE: To characterize PGRMC1 and SERBP1 in human endometrium and to investigate the putative role of PGRMC1 in endometrial decidualization. DESIGN: The PGRMC1 and SERBP1 expression in human endometrium was determined throughout the menstrual cycle. We analyzed the colocalization of PGRMC1 and SERBP1. Then, endometrial stromal cells (ESCs) were isolated to investigate the functional effect of PGRMC1 overexpression on decidualization. SETTING: IVI clinic...
September 11, 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28904504/role-of-serpine-gene-polymorphism-in-recurrent-implantation-failure-and-preeclampsia
#9
Nidhi Sharma, Jayashree K Srinivasan, Margeret Harriet Priya, Venkatachalam Sibiya
This is a rare case of serpine gene polymorphism causing thrombophilia and recurrent implantation failure following intrauterine insemination. SERPINE1 gene encodes plasminogen activator inhibitor type 1 and inhibits fibrinolysis, or clot dissolution. The 4G variant results in increased expression of SERPINE1 and consequently higher inhibition of fibrinolysis, thus leading to thrombophilia. The patient had unexplained primary infertility for 9 years. Ovulation induction was done with gonadotropin releasing hormone (GnRH) agonist long protocol...
April 2017: Journal of Human Reproductive Sciences
https://www.readbyqxmd.com/read/28888606/preovulatory-follicle-characteristics-and-oocyte-competence-in-repeat-breeder-dairy-cows
#10
P Sood, M Zachut, I Dekel, H Dube, S Jacoby, U Moallem
The varied and elusive etiology of repeat breeding (RB) in dairy cows necessitates evaluation of oocytes and follicles, which have not previously been assessed together. Accordingly, we evaluated characteristics of preovulatory follicles and the competence of oocytes in control (CTL) and RB cows. The estrous cycles of 35 cows (18 CTL and 17 RB) were synchronized using PGF2α and estrus detection. Cows with a corpus luteum were treated with PGF2α and, 14 to 15 d after a visible behavioral estrus, they were administered a second PGF2α, followed 48 h later by follicular fluid (FF) aspiration of the preovulatory follicles...
November 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28865549/effect-of-oocyte-vitrification-on-embryo-quality-time-lapse-analysis-and-morphokinetic-evaluation
#11
Ana Cobo, Aila Coello, Jose Remohí, Jose Serrano, Jose Maria de Los Santos, Marcos Meseguer
OBJECTIVE: To analyze whether oocyte vitrification may affect subsequent embryo development from a morphokinetic standpoint by means of time-lapse imaging. DESIGN: Observational cohort study. SETTING: University-affiliated private IVF center. PATIENT(S): Ovum donation cycles conducted with the use of vitrified (n = 631 cycles; n = 3,794 embryos) or fresh oocytes (n = 1,359 cycles; n = 9,935 embryos) over 2 years. INTERVENTIONS(S): None...
September 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28854741/zona-pellucida-protein-b2-messenger-ribonucleic-acid-expression-varies-with-follicular-development-and-granulosa-cell-location
#12
A P Benson, M N Malloy, J R Steed, V L Christensen, B D Fairchild, A J Davis
The freshly ovulated ovum in avian species is surrounded by a protein layer called the inner perivitelline layer (IPVL). The IPVL contains zona pellucida proteins and 6 distinct zona pellucida genes have been identified (ZPA, ZPB1, ZPB2, ZPC, ZPD and ZPX1) in the chicken. In the present research, the expression of the mRNA for ZPA, ZPB2, and ZPX1 was investigated in 2 lines of turkey hens selected for either increased egg production (E line) or increased body weight (F line). Theca and granulosa cell expression of the mRNA for ZPA and ZPB2 was also investigated in hierarchical and prehierarchical follicles from broiler breeder hens...
September 1, 2017: Poultry Science
https://www.readbyqxmd.com/read/28822904/the-effect-of-age-and-length-of-gonadotropin-stimulation-on-the-in%C3%A2-vitro-embryo-development-of-holstein-calf-oocytes
#13
Luke Currin, Laura Michalovic, Anne-Marie Bellefleur, Karina Gutierrez, Werner Glanzner, Yasmin Schuermann, Rodrigo C Bohrer, Naomi Dicks, Paulo R da Rosa, Matheus P De Cesaro, Rosalba Lopez, François-Xavier Grand, Christian Vigneault, Patrick Blondin, Jim Gourdon, Hernan Baldassarre, Vilceu Bordignon
The use of oocytes recovered from prepubertal donors for in vitro embryo production has great potential for accelerating the rate of genetic gain in the dairy industry. However, these oocytes are known to be less developmentally competent than those from adult donors. In this study, we investigated the effect of age and gonadotropin stimulation in Holstein heifers subjected to oocyte collection every two weeks between 2 and 6 months of age. In order to assess the effect of gonadotropin stimulation, animals were subjected to one of three treatments, namely Short (ST; 36-42 h), Long (LT; ≥72 h) and No Treatment (NT) prior to laparoscopic ovum pick-up (LOPU)...
August 10, 2017: Theriogenology
https://www.readbyqxmd.com/read/28822459/oral-propylene-glycol-modifies-follicular-fluid-and-gene-expression-profiles-in-cumulus-oocyte-complexes-and-embryos-in-feed-restricted-heifers
#14
G Gamarra, C Ponsart, S Lacaze, F Nuttinck, A Cordova, P Mermillod, B Marquant-Le Guienne, D Monniaux, P Humblot, A A Ponter
Dietary supplementation with propylene glycol (PG) increases in vitro production of high-quality embryos in feed-restricted heifers. The aim of the present study was to evaluate the effects of PG in feed-restricted heifers on follicular fluid insulin and insulin-like growth factor (IGF) 1 concentrations, expression of IGF system genes in oocytes and cumulus cells and the expression of selected genes in blastocysts. Feed-restricted (R) heifers were drenched with water or PG during induced oestrous cycles (400mL of PG or water/drench, daily drenching at 1600 hours for the first 9 days of the oestrous cycle)...
August 21, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28795048/evaluation-of-human-embryo-development-in-in-vitro-fertilization-and-intracytoplasmic-sperm-injection-fertilized-oocytes-a-time-lapse-study
#15
Hyung Jun Kim, Hye Jin Yoon, Jung Mi Jang, Won Don Lee, San Hyun Yoon, Jin Ho Lim
OBJECTIVE: We investigated whether the insemination method (in vitro fertilization [IVF] or intracytoplasmic sperm injection [ICSI]) affected morphokinetic events and abnormal cleavage events in embryonic development. METHODS: A total of 1,830 normal fertilized embryos were obtained from 272 IVF and ICSI cycles that underwent ovum retrieval culture using a time-lapse system (Embryoscope) from June 2013 to March 2015. All embryos were investigated by a detailed time-lapse analysis that measured the developmental events in the hours after IVF or ICSI insemination...
June 2017: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/28780914/epicutaneous-sensitization-with-nematode-antigens-of-fish-parasites-results-in-the-production-of-specific-igg-and-ige
#16
G Fontenelle, M Knoff, M A Verícimo, S C São Clemente
Fish consumption plays an important role in the human diet. Hoplias malabaricus, trahira, is a freshwater fish widely appreciated in several Brazilian states and it is frequently infected by Contracaecum multipapillatum third-instar larvae (L3). The aim of the present study was to evaluate the allergenic potential of the C. multipapillatum L3 crude extract (CECM). BALB/c mice were immunized intraperitoneally (ip) with 10 or 50 μg CECM associated with 2 mg of aluminium hydroxide on days 0, 14 and 48. The determination of specific IgG and IgE antibody levels was done after immunization, and the late immunity was evaluated by the intradermal reaction in the ear pavilion...
August 7, 2017: Journal of Helminthology
https://www.readbyqxmd.com/read/28764571/increased-c4d-and-bb-immunoreactivity-and-decreased-mbl-immunoreactivity-characterise-first-time-pathologic-first-trimester-miscarriage-a-case-control-study
#17
M Tunc Canda, Latife Doganay Caglayan, Namik Demir, Ragıp Ortaç
The role of the complement system in first-time pathologic first-trimester miscarriage was investigated. In this case-control study, tissue samples of 126 women with pathologic miscarriage and termination of normal pregnancies were assessed. The pathologic pregnancy group consisted of 40 women with missed miscarriage, 13 women with incomplete miscarriage and 10 women with a blighted ovum. The control group consisted of 63 normal-appearing pregnancies. Immunoreactivity for C4d, Bb and MBL was evaluated in the deciduas and villous trophoblasts separately using a semi-quantitative histological scoring system (H-score)...
August 1, 2017: Journal of Obstetrics and Gynaecology: the Journal of the Institute of Obstetrics and Gynaecology
https://www.readbyqxmd.com/read/28750299/prolonged-activation-of-the-coagulation-system-during-in-vitro-fertilization-cycles
#18
Yoni Cohen, Togas Tulandi, Benny Almog, Eyal Zohav, Varda Deutsch, Ariel Many, Ami Amit, Aviad Cohen
OBJECTIVE: To investigate coagulation system changes during an in-vitro fertilization (IVF) cycle using Thromboelastogram (TEG) that enables analysis of the elastic properties of whole blood samples and provides a global assessment of the hemostatic function. STUDY DESIGN: A prospective study. TEG indices were evaluated in 23 women who underwent controlled ovarian stimulation for IVF at four points in time: 1. At the beginning of the cycle (corresponding to the lowest levels of E2), 2...
September 2017: European Journal of Obstetrics, Gynecology, and Reproductive Biology
https://www.readbyqxmd.com/read/28744919/differential-expression-of-insulin-like-growth-factor-family-members-in-immature-cumulus-oocyte-complexes-from-dairy-cows-with-different-genotypes
#19
A C Lopes, M P Palhão, Cac Fernandes, M J Sudano, Acs Castilho, E S Caixeta
It has been evident the improvement of in vitro embryo production (IVEP) in dairy cows. Nevertheless, it is known that differences in the number and quality of oocytes between taurine and zebu females impact the efficiency and economic viability of IVEP. As the insulin-like growth factor (IGF) system is related to follicular and oocyte development, we aimed to quantify mRNA abundance of IGF system members and pregnancy-associated plasma protein-A (PAPPA) in the cumulus-oocyte complexes (COCs) of Gir, 1/2 Holstein × 1/2 Gir and Holstein cows...
July 25, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28736365/-blighted-ovum-in-subfertile-patients-undergoing-assisted-reproductive-technology
#20
Qing-Wen Nie, Rui Hua, Yao Zhou, Hong Li, Yan-Hong Yu
OBJECTIVE: To explore the incidence and risk factors of blighted ovum in subfertile patients undergoing assisted reproductive technology (ART). METHODS: This retrospective analysis was conducted among 2378 patients who were pregnant following embryo transfer at our center from January, 2012 to December, 2015, including cases of early pregnancy losses and simultaneous live births. The cases with early pregnancy losses were divided into embryonic pregnancy and blighted ovum groups based on the presence or absence of an embryonic pole before dilation and curettage...
July 20, 2017: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
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