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Yuqin Pan, Huiling Sun, Xiuxiu Hu, Bangshun He, Xiangxiang Liu, Tao Xu, Xiaoxiang Chen, Kaixuan Zeng, Shukui Wang
Recently, miR‑485‑5p was identified as a tumor‑suppressing microRNA in some human cancers. However, the expression and biological role of miR‑485‑5p in colorectal cancer (CRC) remain unclear. Herein, we found that the expression of miR‑485‑5p in CRC tissues and cell lines was much lower than that in the control, respectively. Moreover, the overexpression of miR‑485‑5p could inhibit the proliferation and invasion of CRC cell lines in vitro and in vivo. Furthermore, we determined that miR‑485‑5p could decrease the expression of CD147 by directly targeting CD147 3'UTR...
March 12, 2018: Oncology Reports
Chun Feng, Zhaobo He, Bo Cai, Jianhong Peng, Jieping Song, Xuechen Yu, Yue Sun, Jing Yuan, Xingzhong Zhao, Yuanzhen Zhang
Detection of detached fetal nucleated red blood cells (fNRBCs) in the maternal peripheral blood may serve as a prospective testing method competing with the cell-free DNA, in non-invasive prenatal testing (NIPT). Methods: Herein, we introduce a facile and effective lab-on-a-chip method of fNRBCs detection using a capture-releasing material that is composed of biotin-doped polypyrrole nanoparticles. To enhance local topographic interactions between the nano-components and fNRBC, a specific antibody, CD147, coated on the nanostructured substrate led to the isolation of fNRBCs from maternal peripheral blood...
2018: Theranostics
Tadashi Asakura, Masayuki Yokoyama, Koichi Shiraishi, Katsuhiko Aoki, Kiyoshi Ohkawa
BACKGROUND: CD147 (basigin/emmprin) is expressed on the surface of carcinoma cells. MATERIALS AND METHODS: For studying the efficacy of CD147-targeting medicine on CD147-expressing cells, we studied the effect of anti-CD147-labeled polymeric micelles (CD147ab micelles) that encapsulated a conjugate of doxorubicin with glutathione (GSH-DXR), with specific accumulation and cytotoxicity against CD147-expressing A431 human epidermoid carcinoma cells, Ishikawa human endometrial adenocarcinoma cells, and PC3 human prostate carcinoma cells...
March 2018: Anticancer Research
Chenxi Qu, Jizhao Li, Yejuan Zhou, Shudi Yang, Weiliang Chen, Fang Li, Bengang You, Yang Liu, Xuenong Zhang
Low accumulation in tumor sites and slow intracellular drug release remain as the obstacles for nanoparticles to achieve effective delivery of chemotherapeutic drugs. In this study, multifunctional micelles were designed to deliver doxorubicin (Dox) to tumor sites to provide more efficient therapy against hepatic carcinoma. The micelles were based on pH-responsive carboxymethyl chitosan (CMCh) modified with a reactive oxygen species (ROS)-responsive segment phenylboronic acid pinacol ester (BAPE) and an active targeted ligand CD147 monoclonal antibody...
February 23, 2018: AAPS Journal
Xu Zhang, Lisha Wu, Ta Xiao, Ling Tang, Xuekun Jia, Yeye Guo, JiangLin Zhang, Jie Li, Yijing He, Juan Su, Shuang Zhao, Juan Tao, Jianda Zhou, Xiang Chen, Cong Peng
TRAF6, a well-known adapter molecule, plays pivotal role in TLR/IL-1R associated signaling pathway. Although TRAF6 has been shown to have oncogenic activity in various malignant tumors, the details remain unclear. In this study, we demonstrated that TRAF6 facilitates Ras (G12V) and EGF-induced cellular transformation through EGFR. Silencing of TRAF6 expression significantly downregulated AP-1 activity, as well as MMP-2,9 expression after EGF stimulation. Furthermore, we found that TRAF6 plays an essential role in cutaneous squamous cell carcinoma (cSCC) malignant phenotypes, affecting cell growth and migration...
February 20, 2018: Oncogenesis
Weiming Wang, Haofeng Xiong, Zhengmao Hu, Rongjuan Zhao, Yue Hu, Wenxin Chen, Ying Han, Liudi Yang, Xin Hu, Can Wang, Ting Mao, Kun Xia, Tong Su
OBJECTIVE: Although previous evidence indicates that CD147 is closely involved in the progression of organ fibrosis and various signaling pathways have been proven to regulate its expression, the role of CD147 in oral submucous fibrosis (OSF) remain largely unknown. METHODS: In the present study, we investigated the expression of CD147 and transforming growth factor β1 (TGF-β1) in human samples of an OSF tissue array by immunohistopathology. Pearson's correlation analysis was conducted to explore the correlation between CD147 and TGF-β1...
February 19, 2018: Oral Diseases
Tian Zhang, Hao Li, Yang Zhang, Pei Wang, Huijie Bian, Zhi-Nan Chen
Cluster of differentiation 147 (CD147), pituitary tumor transforming gene (PTTG) and CD44v6 are proteins involved in the epithelial-mesenchymal transition (EMT). To investigate the prognostic value of CD147 and PTTG, and CD44v6 expression in esophageal squamous cell carcinoma (ESCC), tissue microarray specimens from 76 patients with ESCC were evaluated by immunohistochemistry staining and scored by intensity and proportion of positive areas. Expression levels of CD147, PTTG and CD44v6 were higher in tumor tissues than in matched adjacent tissues...
March 2018: Oncology Letters
Jian Cui, Wan Huang, Bo Wu, Jin Jin, Lin Jing, Wen-Pu Shi, Zhen-Yu Liu, Lin Yuan, Dan Luo, Ling Li, Zhi-Nan Chen, Jian-Li Jiang
While the importance of protein N-glycosylation in cancer cell migration is well appreciated, the precise mechanisms by which N-acetylglucosaminyltransferase V (GnT-V) regulates cancer processes remain largely unknown. In the current study, we report that GnT-V mediated N-glycosylation of CD147/Basigin, a tumor-associated glycoprotein that carries β1,6 N-acetylglucosamine (β1,6 GlcNAc) glycans, is upregulated during TGF-β1-induced epithelial-to-mesenchymal transition (EMT), which correlates with tumor metastasis in patients with hepatocellular carcinoma (HCC)...
February 12, 2018: Journal of Pathology
Jun-Chao Xie, Xiao-Ye Ma, Xiao-Hui Liu, Jia Yu, Yi-Chen Zhao, Yan Tan, Xue-Yuan Liu, Yan-Xin Zhao
Hypoxia promotes the accumulation of amyloid-β (Aβ), which is related to the pathogenesis of Alzheimer's disease (AD). CD147 is considered as an additional subunit of γ-secretase regulated by hypoxia, and has been identified in exosomes. Aβ is also found in exosomes that participate in the intercellular communication and amyloids propagation. This study was to investigate the role of CD147 in hypoxia-induced accumulation of Aβ in exosomes. Our results showed that hypoxia increased the levels of Aβ40 and Aβ42 in exosomes and enhanced the interaction between CD147 and Hook1 in SH-SY5YAPP695 cells...
2018: American Journal of Translational Research
Xin Yu, Jingcheng Miao, Wei Xia, Zong-Jiang Gu
Previous studies have shown that interleukin-24 (IL-24) has tumor-suppressing activity by multiple pathways. However, the immunogenicity moderation effect of IL-24 on malignant cells has not been explored extensively. In this study, we investigated the role of IL-24 in immunogenicity modulation of the myelogenous leukemia cells. Data show that myelogenous leukemia cells express low levels of immunogenicity molecules. Treatment with IL-24 could enhance leukemia cell immunogenicity, predominantly regulate leukemia cells to produce immune-associated cytokines, and improve the cytotoxic sensitivity of these cells to immune effector cells...
February 7, 2018: Anti-cancer Drugs
Saskia N I von Ungern-Sternberg, Alma Zernecke, Peter Seizer
The receptor EMMPRIN is involved in the development and progression of cardiovascular diseases and in the pathogenesis of myocardial infarction. There are several binding partners of EMMPRIN mediating the effects of EMMPRIN in cardiovascular diseases. EMMPRIN interaction with most binding partners leads to disease progression by mediating cytokine or chemokine release, the activation of platelets and monocytes, as well as the formation of monocyte-platelet aggregates (MPAs). EMMPRIN is also involved in atherosclerosis by mediating the infiltration of pro-inflammatory cells...
February 8, 2018: International Journal of Molecular Sciences
Wenbo Li, Wei Liu, Can Chen, Wenhui Fan, He Zhang, Wenjun Liu, Lei Sun
Cyclophilin A (CypA) is a member of peptidyl prolylisomerases (PPIase) family. CypA is best known as a ubiquitously distributed intracellular protein. It has also been shown to be secreted by cells in response to inflammatory stimuli and oxidative stress. Extracellular CypA (eCypA) interacts with CD147 to initiate inflammatory responses via recruiting leucocytes into inflamed tissue. Recombinant CypA was expressed in Escherichia coli and then purified using Superdex 75™ 16/60. The results of Real-time PCR and ELISA showed that the expression levels of proinflammatory cytokines, such as IL-1β, secreted by eCypA stimulated BMDM were significantly up-regulated, indicating that eCypA played an important role in promoting inflammatory responses...
January 25, 2018: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
Ning-Yu Ru, Long-Biao Cui, Bo Jiao, Lin Zhang, Shuai Jiang, Zhi-Bin Yu
The mechanism of transition from chronic pressure overload-induced cardiac hypertrophy to heart failure is still unclear. Angiotensin II (Ang II) may be an important factor that mediates the transition in the end-stage of cardiac hypertrophy. In the present study, Goldblatt two-kidney one-clip (2K1C) rat model was used to simulate Ang II-induced hypertension. The elevated Ang II not only induced the concentric hypertrophy of left ventricle and cardiac fibrosis, but also increased the expression and glycosylation of CD147 in 2K1C rats...
January 29, 2018: Journal of Cellular Biochemistry
Anne Poder Andersen, Jacob Samsøe-Petersen, Eva Kjer Oernbo, Ebbe Boedtkjer, José M A Moreira, Marie Kveiborg, Stine Falsig Pedersen
High metabolic and proliferative rates in cancer cells lead to production of large amounts of H+ and CO2 , and as a result, net acid extruding transporters are essential for the function and survival of cancer cells. We assessed protein expression of the Na+ /H+ exchanger NHE1, the Na+ -HCO3- cotransporter NBCn1, and the lactate-H+ cotransporters MCT1 and -4 by immunohistochemical analysis of a large cohort of breast cancer samples. We found robust expression of these transporters in 20, 10, 4 and 11 percent of samples, respectively...
January 24, 2018: International Journal of Cancer. Journal International du Cancer
Meng Lu, Jiao Wu, Zhi-Wei Hao, Yu-Kui Shang, Jing Xu, Gang Nan, Xia Li, Zhi-Nan Chen, Huijie Bian
Hepatocytes are epithelial cells with highly specialized polarity. The disorder and loss of hepatocyte polarity lead to the weakness of cell adhesion and connection, the induction of epithelial-mesenchymal transition and eventually the occurrence of hepatocellular carcinoma (HCC). CD147, a tumor-related glycoprotein, promotes epithelial-mesenchymal transition and the invasion of HCC. However, the function of CD147 in hepatocyte depolarization is unknown. Here we identified that CD147 was basolaterally polarized in hepatocyte membrane of liver tissues and HepG2 cells...
January 21, 2018: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
Meng-Yao Zhang, Yang Zhang, Xiao-Dong Wu, Kun Zhang, Peng Lin, Hui-Jie Bian, Min-Min Qin, Wan Huang, Ding Wei, Zhao Zhang, Jiao Wu, Ruo Chen, Fei Feng, Bin Wang, Gang Nan, Ping Zhu, Zhi-Nan Chen
Effective vaccines against Plasmodium falciparum malaria are still lacking and the molecular mechanism of the host-parasite interaction is not fully understood. Here we demonstrate that the interaction of RAP2, a parasite secreted rhoptry protein functioning in the parasitophorous vacuole formation stage of the invasion, and CD147 on host erythrocyte is essential for erythrocyte invasion by Plasmodium falciparum and is independent from all previously identified interactions involved. Importantly, the blockade of the CD147-RAP2 interaction by HP6H8, a humanized CD147 antibody, completely abolished the parasite invasion with both cure and preventative functions in a humanized mouse model...
January 19, 2018: Blood
Orçun Haçarız, Gearóid P Sayers
A total of 71 virulence and immunomodulation-related transcripts (VIRs) of Fasciola hepatica have been previously proposed (Haçarız et al., 2015). In an attempt to further refine this cohort, an in silico meta analysis approach was carried out using publicly available sequence data of related liver flukes, Clonorchis sinensis and Opisthorchis viverrini. Data of both liver flukes were investigated in terms of sequential homology with data of non-parasitic organisms, pathogens and VIRs of F. hepatica, directional selection (Ka/Ks), and cytokine signaling relation (protein motif based)...
December 28, 2017: Research in Veterinary Science
Andrea Cruzat, Miguel Gonzalez-Andrades, Jérôme Mauris, Dina B AbuSamra, Preethi Chidambaram, Kenneth R Kenyon, James Chodosh, Claes H Dohlman, Pablo Argüeso
Purpose: Galectin-3 is a carbohydrate-binding protein known to promote expression of matrix metalloproteinases, a hallmark of ulceration, through interaction with the extracellular matrix metalloproteinase inducer CD147. The aim of this study was to investigate the distribution of galectin-3 in corneas of patients with ulcerative keratitis and to determine its relationship to CD147 and the presence of gelatinolytic activity. Methods: This was an observational case series involving donor tissue from 13 patients with active corneal ulceration and 6 control corneas...
January 1, 2018: Investigative Ophthalmology & Visual Science
Eliana Amati, Omar Perbellini, Gianluca Rotta, Martina Bernardi, Katia Chieregato, Sabrina Sella, Francesco Rodeghiero, Marco Ruggeri, Giuseppe Astori
BACKGROUND: Mesenchymal stromal cells (MSC) are a heterogeneous population of multipotent progenitors used in the clinic because of their immunomodulatory properties and their ability to differentiate into multiple mesodermal lineages. Although bone marrow (BM) remains the most common MSC source, cord blood (CB) can be collected noninvasively and without major ethical concerns. Comparative studies comprehensively characterizing the MSC phenotype across several tissue sources are still lacking...
January 16, 2018: Stem Cell Research & Therapy
Ye Tian, Ling Ma, Manfei Gong, Guoqiang Su, Shaobin Zhu, Wenqiang Zhang, Shuo Wang, Zhibin Li, Chaoxiang Chen, Lihong Li, Lina Wu, Xiaomei Yan
Extracellular vesicles (EVs) have stimulated considerable scientific and clinical interest, yet protein profiling and sizing of individual EVs remains challenging due to their small particle size, low abundance of proteins, and overall heterogeneity. Building upon a laboratory-built high-sensitivity flow cytometer (HSFCM), we report here a rapid approach for quantitative multiparameter analysis of single EVs down to 40 nm with an analysis rate up to 10,000 particles per minute. Statistically robust particle size distribution was acquired in minutes with a resolution and profile well matched with those of cryo-TEM measurements...
January 4, 2018: ACS Nano
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