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Víctor Molina, Bettina von Dessauer, Ramón Rodrigo, Cristian Carvajal
OBJECTIVES: Oxidative stress is known to participate in the progression of sepsis. Definite data regarding the behavior of oxidative stress biomarkers in pediatric sepsis is still lacking. This study hypothesized that oxidative stress occurs in pediatric sepsis and that the magnitude of the redox derangement is associated with worse clinical progression. METHODS: Forty-two previously healthy pediatric patients with sepsis and a group of control subjects were included...
October 13, 2016: Redox Report: Communications in Free Radical Research
Fang Zhang, Guiyou Liu, Yali Bu, Xiaofeng Ma, Junwei Hao
For the epigenetic characterization of myasthenia gravis (MG), we determined whether long noncoding RNAs (lncRNAs) and messenger RNAs (mRNAs) are expressed differentially in subjects with and without MG. Compared with healthy control subjects, the MG patients had 1561 upregulated lncRNAs, 1034 downregulated lncRNAs, 921 upregulated mRNAs, and 806 downregulated mRNAs (fold change>2.0). Several GO terms including nucleic acid transcription factor activity, inflammatory response, regulation of leukocyte activation, lymphocyte proliferation and regulation of B cell proliferation were enriched in gene lists, suggesting a potential correlation with MG...
October 15, 2016: Journal of Neuroimmunology
Qingchang Hu, Yabo Zhao, Zaiwei Wang, Yue Hou, Dingren Bi, Jianjun Sun, Xiuli Peng
Mycoplasma gallisepticum (MG), one of the most pathogenic Mycoplasmas, can cause chronic respiratory disease (CRD) in chickens. It has been suggested that micro-ribonucleic acids (miRNAs) are involved in microbial pathogenesis. However, little is known about the roles of miRNAs in MG infection. Previously, we found by deep sequencing that gga-miR-19a was significantly up-regulated in the lungs of MG-infected chicken embryos. In this work, we confirmed that gga-miR-19a was up-regulated in both MG-infected chicken embryonic lungs and MG-infected DF-1 (chicken embryo fibroblast) cells...
2016: Frontiers in Cellular and Infection Microbiology
Zhen Tan, Jianwen Cheng, Qian Liu, Lin Zhou, Jacob Kenny, Tao Wang, Xixi Lin, Jinbo Yuan, Julian M W Quinn, Jennifer Tickner, Guoju Hong, An Qin, Jinmin Zhao, Jiake Xu
Excessive bone resorption by osteoclasts plays an important role in osteoporosis. Bone loss occurs in ovariectomised (OVX) mice in a similar manner to that in humans, so this model is suitable for evaluating potential new therapies for osteoporosis. Neohesperidin (NE) is a flavonoid compound isolated from citrus fruits. Its role in bone metabolism is unknown. In this study we found that neohesperidin inhibits osteoclast differentiation, bone resorption and the expression of osteoclast marker genes, tartrate-resistant acid phosphatase and cathepsin K...
September 21, 2016: Molecular and Cellular Endocrinology
Shengqian Yang, Ziru Yu, Tianyi Yuan, Lin Wang, Xue Wang, Haiguang Yang, Lan Sun, Yuehua Wang, Guanhua Du
Acute lung injury (ALI), characterized by pulmonary edema and inflammatory cell infiltration, is a common syndrome of acute hypoxemic respiratory failure. Methyl salicylate 2-O-β-d-lactoside (MSL), a natural derivative of salicylate extracted from Gaultheria yunnanensis (Franch.) Rehder, was reported to have potent anti-inflammatory effects on the progression of collagen or adjuvant-induced arthritis in vivo and in vitro. The aim of this study is to investigate the therapeutic effect of MSL on lipopolysaccharide (LPS)-induced acute lung injury and reveal underlying molecular mechanisms...
September 8, 2016: International Immunopharmacology
Wei Huang, Xiuying Cui, Jianing Chen, Yuhuan Feng, Erwei Song, Jinsong Li, Yujie Liu
Long non-coding RNAs (lncRNAs) have emerged recently as key regulators of tumor development and progression. Our previous study identified an NF-KappaB interacting lncRNA (NKILA) which was negatively correlated with breast cancer metastasis and patient prognosis. However, its clinical significance and potential role in Tongue squamous cell carcinoma (TSCC) remain unclear. Here we show that NKILA is down-regulated in TSCC cancer tissues than that in matched adjacent noncancerous tissues. And low NKILA expression in TSCC is significantly correlated with tumor metastasis and poor patient prognosis...
August 23, 2016: Oncotarget
Brandon Cieniewicz, Alexis L Santana, Nana Minkah, Laurie T Krug
Herpesviruses establish a chronic infection in the host characterized by intervals of lytic replication, quiescent latency, and reactivation from latency. Murine gammaherpesvirus 68 (MHV68) naturally infects small rodents and has genetic and biologic parallels with the human gammaherpesviruses (gHVs), Kaposi's sarcoma-associated herpesvirus and Epstein-Barr virus. The murine gammaherpesvirus model pathogen system provides a platform to apply cutting-edge approaches to dissect the interplay of gammaherpesvirus and host determinants that enable colonization of the host, and that shape the latent or lytic fate of an infected cell...
2016: Frontiers in Microbiology
Da-Long Yin, Ying-Jian Liang, Tong-Sen Zheng, Rui-Peng Song, Jia-Bei Wang, Bo-Shi Sun, Shang-Ha Pan, Lian-Dong Qu, Jia-Ren Liu, Hong-Chi Jiang, Lian-Xin Liu
A synthetic monoketone analog of curcumin, termed 3, 5-bis (2-flurobenzylidene) piperidin-4-one (EF24), has been reported to inhibit the growth of a variety of cancer cells both in vitro and in vivo. However, whether EF24 has anticancer effects on cholangiocarcinoma (CCA) cells and the mechanisms remain to be investigated. The aim of our study was to evaluate the molecular mechanisms underlying the anticancer effects of EF24 on CCA tumor growth and metastasis. Cell proliferation, apoptosis, migration, invasion, tumorigenesis and metastasis were examined...
2016: Scientific Reports
Yasunari Nakamoto
Hepatocellular carcinoma (HCC) is one of the most common causes of cancer death worldwide. It usually arises based on a background of chronic liver diseases, defined as the hypercarcinogenic state. The current treatment options for HCC ranging from locoregional treatments to chemotherapies, including sorafenib, effectively regulate the limited sizes and numbers of the nodules. However, these treatments remain unsatisfactory because they have insufficient antitumor effects on the large and numerous nodules associated with HCC and because of a high recurrence rate in the surrounding inflamed liver...
August 25, 2016: Hepatology Research: the Official Journal of the Japan Society of Hepatology
Zhifeng Zhang, Yuan Liu, Xuecong Ren, Hua Zhou, Kaishun Wang, Hao Zhang, Pei Luo
Erigeron multiradiatus (Lindl.) Benth. has been used in Tibet folk medicine to treat various inflammatory diseases. The aim of this study was to investigate antimyocardial ischemia and reperfusion (I/R) injury effect of caffeoylquinic acids derivatives of E. multiradiatus (AE) in vivo and to explain underling mechanism. AE was prepared using the whole plant of E. multiradiatus and contents of 6 caffeoylquinic acids determined through HPLC analysis. Myocardial I/R was induced by left anterior descending coronary artery occlusion for 30 minutes followed by 24 hours of reperfusion in rats...
2016: Mediators of Inflammation
Hon Wai Koon, Bowei Su, Chunlan Xu, Caroline C Mussatto, Diana Hoang-Ngoc Tran, Elaine C Lee, Christina Ortiz, Jiani Wang, Jung Eun Lee, Samantha Ho, Xinhua Chen, Ciaran P Kelly, Charalabos Pothoulakis
C. difficile infection (CDI) is a common debilitating nosocomial infection associated with high mortality. Several CDI outbreaks have been attributed to ribotypes 027, 017, and 078. Clinical and experimental evidence indicates that the nonpathogenic yeast Saccharomyces boulardii CNCM I-745 (S.b) is effective for the prevention of CDI. However, there is no current evidence suggesting this probiotic can protect from CDI caused by outbreak-associated strains. We used established hamster models infected with outbreak-associated C...
October 1, 2016: American Journal of Physiology. Gastrointestinal and Liver Physiology
Weibin Qian, Xinrui Cai, Xinying Zhang, Yingying Wang, Qiuhai Qian, Junichi Hasegawa
BACKGROUND: Daisaikoto (DSKT), a classical traditional Chinese herbal formula, has been used for treating digestive diseases for 1800 years in China. Therefore, in this study, we are going to investigate the effect of DSKT on diabetic fatty liver rats induced by a high-fat diet and streptozotocin (STZ), and the effects of DSKT on silent mating type information regulation 2 homolog 1 (SIRT1) and nuclear factor kappa B (NF-kappaB). METHODS: Diabetic fatty liver rat model was selected to establish a high-fat diet and STZ...
June 2016: Yonago Acta Medica
Elizaveta Fasler-Kan, Yeldar Baiken, Ivan A Vorobjev, Natasha S Barteneva
Many intracellular signal transduction events involve the reversible shuttling of proteins between the cytoplasm and the nucleus. Study of these processes requires imaging information on the protein localization in a given cell and a large number of measurements to obtain sufficient statistics on the protein localization in the whole population. The protocol describes method for quantitative imaging flow cytometry analysis of intracellular distribution of NF-kappaB in ARPE-19 cells stained with specific fluorochrome-conjugated antibodies...
2016: Methods in Molecular Biology
Xiaoming Gong, Raju Marisiddaiah, Susan Zaripheh, Doris Wiener, Lewis P Rubin
: Despite numerous inquiries into protective roles of lycopene in prostate cancer (PCa) prevention or therapy, little is known about mechanisms by which lycopene or its metabolites inhibit PCa. The enzyme beta-carotene 9,10'-oxygenase (BCO2), which catalyzes asymmetric cleavage of several carotenoids, is the principal regulator of lycopene metabolism, but the range of BCO2 biological functions is incompletely understood. This study investigated expression and functional roles of BCO2 in human PCa...
July 12, 2016: Molecular Cancer Research: MCR
Savita Bisht, Martin Schlesinger, Alexander Rupp, Rolf Schubert, Jens Nolting, Jörg Wenzel, Stefan Holdenrieder, Peter Brossart, Gerd Bendas, Georg Feldmann
BACKGROUND: Pancreatic cancer is one of the most lethal of human malignancies known to date and shows relative insensitivity towards most of the clinically available therapy regimens. 3,5-bis(2-fluorobenzylidene)-4-piperidone (EF24), a novel synthetic curcumin analog, has shown promising in vitro therapeutic efficacy in various human cancer cells, but insufficient water solubility and systemic bioavailability limit its clinical application. Here, we describe nano-encapsulation of EF24 into pegylated liposomes (Lipo-EF24) and evaluation of these particles in preclinical in vitro and in vivo model systems of pancreatic cancer...
2016: Journal of Nanobiotechnology
Lucia Messingerova, Denisa Imrichova, Helena Kavcova, Mario Seres, Zdena Sulova, Albert Breier
We established an azacytidine (AzaC)-resistant human acute myeloid leukemia (AML) cell line (SKM-1/AzaC) by culturing SKM-1 cells in the presence of increasing amounts of AzaC for six months. Because AzaC is not a substrate of P-glycoprotein (a product of the ABCB1 gene; ABCB1), ABCB1 was not responsible for AzaC resistance; nevertheless, it was notably upregulated in SKM-1/AzaC cells. In addition, the transcription of the Nfkb1 gene, which encodes a member of the canonical NF-kappaB regulatory pathway, was downregulated, and the transcription of the Nfkb2 gene, which encodes a member of the non-canonical NF-kappaB regulatory pathway, was upregulated in SKM-1/AzaC cells...
October 2016: Toxicology in Vitro: An International Journal Published in Association with BIBRA
Wu-kai Ma, Dao-min Lu, Xue-ming Yao, Ying Huang, Fang Tang, Jiang Liang, Yang An, Jing Zhou
OBJECTIVE: To explore the effect of Jinwu Jiangu Recipe (JJR) on the expression of synovial cells' nuclear factor-kappaB (NF-kappaB) and serum interleukin 17 (IL-17) in collagen induced arthritis (CIA) rats. METHODS: Totally 60 Wistar rats were randomly divided into 6 groups, i.e., the blank control group, the model group, high, middle, and low dose JJR treatment groups, and the tripterygium control group, 10 in each group. Except rats in the blank control group, CIA model was established in rats of the rest 5 groups...
May 2016: Chinese Journal of Integrated Traditional and Western Medicine
Sabrina Pagano, Federico Carbone, Fabienne Burger, Aline Roth, Maria Bertolotto, Bianca Pane, Giovanni Spinella, Domenico Palombo, Aldo Pende, Franco Dallegri, Nathalie Satta, Julien Virzi, Pierre Fontana, François Mach, Fabrizio Montecucco, Nicolas Vuilleumier
Humoral autoimmune-mediated inflammation plays a role in atherogenesis, and potentially in arterial thrombosis. Anti-apolipoprotein A-1 (apoA-1) IgG have been reported to represent emergent mediators of atherogenesis through Toll-like receptors (TLR) 2, 4 and CD14 signalling. We investigated the role of anti-apoA-1 IgG on tissue factor (TF) expression and activation, a key coagulation regulator underlying atherothrombosis. Atherothrombosis features were determined by immunohistochemical TF staining of human carotid biopsies derived from patients with severe carotid stenosis undergoing elective surgery (n=176), and on aortic roots of different genetic backgrounds mice (ApoE-/-; TLR2-/-ApoE-/- and TLR4-/-ApoE-/-) exposed to passive immunisation with anti-apoA-1 IgG...
August 30, 2016: Thrombosis and Haemostasis
Allen Mo, Stephen Jackson, Kamini Varma, Alan Carpino, Charles Giardina, Thomas J Devers, Daniel W Rosenberg
UNLABELLED: Although the progression of mutated colonic cells is dependent upon interactions between the initiated epithelium and surrounding stroma, the nature of these interactions is poorly understood. Here, the development of an ultrasensitive laser capture microdissection (LCM)/RNA-seq approach for studying the epithelial and stromal compartments of aberrant crypt foci (ACF) is described. ACF are the earliest identifiable preneoplastic lesion found within the human colon and are detected using high-definition endoscopy with contrast dye spray...
September 2016: Molecular Cancer Research: MCR
Sumit Kumar, Ramovatar Meena, R Paulraj
Titanium-dioxide nanoparticles (TNP) are used in various consumable goods. Evidence has demonstrated the cytotoxicity of TNPs, but exact mechanism is yet to be elucidated. The present study has been aimed at finding out the mechanism of TNP-induced toxicity in biological system. Different doses of anatase-TNPs administrated intravenously to Wistar rats for once a week for 1 month and properties of TH cells, macrophages, cytokines secretion, oxidative damage, apoptotic pathway, and hematological and pathological changes were investigated as downstream events of TNP-mediated cytotoxicity...
June 18, 2016: Applied Biochemistry and Biotechnology
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