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https://www.readbyqxmd.com/read/28330329/diversity-of-bacteria-and-archaea-in-the-rhizosphere-of-bioenergy-crop-jatropha-curcas
#1
Garima Dubey, Bharati Kollah, Vijay Kumar Gour, Arvind Kumar Shukla, Santosh Ranjan Mohanty
Plant-microbial interaction in rhizosphere plays vital role in shaping plant's growth and ecosystem function. Most of the rhizospheric microbial diversity studies are restricted to bacteria. In natural ecosystem, archaea also constitutes a major component of the microbial population. However, their diversity is less known compared to bacteria. Experiments were carried out to examine diversity of bacteria and archaea in the rhizosphere of bioenergy crop Jatropha curcas (J. curcas). Samples were collected from three locations varying widely in the soil physico-chemical properties...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28327345/immunoassays-for-riboflavin-and-flavin-mononucleotide-using-antibodies-specific-to-d-ribitol-and-d-ribitol-5-phosphate
#2
G Ravi, Yeldur P Venkatesh
Riboflavin (vitamin B2), a water-soluble vitamin, plays a key role in maintaining human health. Though, numerous methods have been reported for the determination of total riboflavin (TRF) content in foods and biological samples, very few methods are reported for quantifying riboflavin and its coenzymes [flavin mononucleotide (FMN); flavin adenine dinucleotide (FAD)] individually. Recently, we have demonstrated that antibodies specific to d-ribitol and d-ribitol-5-phosphate also recognize riboflavin and FMN, respectively, and not vice-versa...
March 18, 2017: Journal of Immunological Methods
https://www.readbyqxmd.com/read/28324494/analysis-of-average-telomere-length-in-human-telomeric-protein-knockout-cells-generated-by-crispr-cas9
#3
Jun Xu, Zhou Songyang, Dan Liu, Hyeung Kim
Telomeres play an important role in ensuring the integrity of the genome. Telomere shortening can lead to loss of genetic information and trigger DNA damage responses. Cultured mammalian cells have served as critical model systems for studying the function of telomere binding proteins and telomerase. Tremendous heterogeneity can be observed both between species and within a single cell population. Recent advances in genome editing (such as the development of the CRISPR/Cas9 platform) have further enabled researchers to carry out loss-of-function analysis of how disrupting key players in telomere maintenance affects telomere length regulation...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28318843/development-of-a-conceptual-etiological-model-of-treatment-regimen-fatigue-among-patients-engaged-in-hiv-care-a-qualitative-study
#4
Kasey Claborn, Mary Beth Miller, Ellen Meier, Sofia Carbone
Treatment regimen fatigue (TRF) is a decreased desire and motivation to maintain vigilance in adhering to treatment, and little is known about TRF in people living with HIV. We aimed to develop a conceptual framework of TRF. Five focus groups were conducted in 2014. Eligible participants were (a) HIV infected, (b) at least 18 years of age, (c) prescribed antiretroviral therapy, and (d) fluent in English. Data were analyzed using thematic analysis. Analyses revealed these themes: patient experiences of TRF; etiological factors at the systems, provider, and patient levels; strategies to manage TRF; consequences of TRF; and protective factors that prevent the occurrence of TRF...
March 3, 2017: Journal of the Association of Nurses in AIDS Care: JANAC
https://www.readbyqxmd.com/read/28314293/assessment-of-nutritional-and-inflammatory-status-to-determine-the-prevalence-of-malnutrition-in-patients-undergoing-surgery-for-colorectal-carcinoma
#5
Antonella Daniele, Rosa Divella, Ines Abbate, Addolorata Casamassima, Vito Michele Garrisi, Eufemia Savino, Porzia Casamassima, Eustachio Ruggieri, Raffaele DE Luca
BACKGROUND/AIM: Colorectal Cancer is the fourth most frequent cause of cancer death worldwide and its incidence increases from 50 years of age. It is often associated with protein-caloric malnutrition and 20% of cancer deaths occur due to this event. The aim of this study was to assess the prevalence of malnutrition and inflammatory status in 78 patients undergoing surgery for colorectal carcinoma. PATIENTS AND METHODS: Nutritional Status was assessed by Mini Nutritional Assessment (MNA)...
March 2017: Anticancer Research
https://www.readbyqxmd.com/read/28299766/increased-level-of-c-kit-in-semen-of-infertile-patients-with-varicocele
#6
Guorong Jin, Jianrong Liu, Qin Qin, Songdan Gao, Fang Zhang, Yuehong Ma, Caiyun Ding, Lina Dong, Haizhen Yin, Yimin Wang
PURPOSE: Varicocele is the most common risk factor for male infertility, however, not all males with varicocele experience infertility. In fact, most patients with varicocele have normal spermatogenesis. The molecular mechanism of varicocele-associated infertility is yet to be completely understood. The aim of this study is to assess the association of a number of fertility regulatory factors on varicocele associated infertility and to throw light on the mechanism of varicocele-associated infertility...
March 16, 2017: Urology Journal
https://www.readbyqxmd.com/read/28295377/time-restricted-feeding-for-prevention-and-treatment-of-cardiometabolic-disorders
#7
Girish C Melkani, Satchidananda Panda
The soaring prevalence of obesity and diabetes is associated with an increase in comorbidities, including elevated risk for cardiovascular diseases (CVD). CVDs continue to be among the leading causes of death and disability in the United States. While increased nutrition intake from an energy dense diet is known to disrupt metabolic homeostasis and contributes to the disease risk, circadian rhythm disruption is emerging as a new risk factor for CVD. Circadian rhythms coordinate cardiovascular health via temporal control of organismal metabolism and physiology...
March 10, 2017: Journal of Physiology
https://www.readbyqxmd.com/read/28277624/plant-phylogeny-as-a-window-on-the-evolution-of-hyperdiversity-in-the-tropical-rainforest-biome
#8
REVIEW
Wolf L Eiserhardt, Thomas L P Couvreur, William J Baker
I. II. III. IV. V. VI. VII. VIII. IX. References SUMMARY: Tropical rainforest (TRF) is the most species-rich terrestrial biome on Earth, harbouring just under half of the world's plant species in c. 7% of the land surface. Phylogenetic trees provide important insights into mechanisms underpinning TRF hyperdiversity that are complementary to those obtained from the fossil record. Phylogenetic studies of TRF plant diversity have mainly focused on whether this biome is an evolutionary 'cradle' or 'museum', emphasizing speciation and extinction rates...
March 9, 2017: New Phytologist
https://www.readbyqxmd.com/read/28259732/prenatal-exposure-to-anxiolytic-and-hypnotic-medication-in-relation-to-behavioral-problems-in-childhood-a-population-based-cohort-study
#9
Maja R Radojčić, Hanan El Marroun, Branislava Miljković, Bruno H C Stricker, Vincent W V Jaddoe, Frank C Verhulst, Tonya White, Henning Tiemeier
Benzodiazepines and benzodiazepine-related medications (BBRMs) are anxiolytics and hypnotics acting on γ-amino butyric acid (GABA)A receptors. BBRMs are assumed to have a low potential for major congenital malformations, but research on more subtle and protracted developing symptoms of these medications is lacking. Therefore, we prospectively investigated the association between BBRM use in pregnancy and long-term effects on child behavior in a large population-based cohort study. The study population consisted of 104 children prenatally exposed to BBRM, 527 children exposed to maternal prenatal anxiety or phobic anxiety symptoms (without exposure to BBRM), and 5609 control children...
March 1, 2017: Neurotoxicology and Teratology
https://www.readbyqxmd.com/read/28243354/tocotrienol-rich-fraction-ameliorates-antioxidant-defense-mechanisms-and-improves-replicative-senescence-associated-oxidative-stress-in-human-myoblasts
#10
Shy Cian Khor, Wan Zurinah Wan Ngah, Yasmin Anum Mohd Yusof, Norwahidah Abdul Karim, Suzana Makpol
During aging, oxidative stress affects the normal function of satellite cells, with consequent regeneration defects that lead to sarcopenia. This study aimed to evaluate tocotrienol-rich fraction (TRF) modulation in reestablishing the oxidative status of myoblasts during replicative senescence and to compare the effects of TRF with other antioxidants (α-tocopherol (ATF) and N-acetyl-cysteine (NAC)). Primary human myoblasts were cultured to young, presenescent, and senescent phases. The cells were treated with antioxidants for 24 h, followed by the assessment of free radical generation, lipid peroxidation, antioxidant enzyme mRNA expression and activities, and the ratio of reduced to oxidized glutathione...
2017: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/28242469/time-restricted-feeding-on-weekdays-restricts-weight-gain-a-study-using-rat-models-of-high-fat-diet-induced-obesity
#11
Magnus Kringstad Olsen, Man Hung Choi, Bård Kulseng, Chun-Mei Zhao, Duan Chen
A recent study reported that a special weekly scheduled time-restricted feeding regimen (TRF), i.e., no food consumption for 15h during the light (inactive) phase per day for 5 weekdays, attenuated the outcome of diverse nutritional challenges in response to high-fat diet in mice. In the present study, we wanted to further test whether this TRF could restrict body weight gain in both juvenile and adult animals when fed a high-fat diet. Fifty male Sprague-Dawley rats at ages from 5 to 27weeks were used. First, we found that freely fed rats with 60% fat diet gained weight significantly, which was associated with more calorie intake (particularly during light phase) than those fed standard food (7% fat)...
February 24, 2017: Physiology & Behavior
https://www.readbyqxmd.com/read/28236320/associations-of-urinary-glomerular-and-tubular-markers-with-the-development-of-diabetic-kidney-disease-in-type-2-diabetes-patients
#12
Xu Jiang, Qian Zhang, Hua-Bin Wang, Xiao-Fan Cui, Rui Liu
OBJECTIVES: To evaluate the associations of urinary markers (eg albumin), glomerular (eg transferrin [TRF], immunoglobulin G [IgG]), and tubular (eg α1-microglobulin [α1-MG], β2-microglobulin [β2-MG]) markers with the development of diabetic kidney disease (DKD) in type 2 diabetes patients, as assessed by estimated glomerular filtration rate (eGFR) and albuminuria. MATERIAL AND METHODS: A total of 252 type 2 diabetes patients and 50 nondiabetic controls from Tianjin, China, were selected...
February 25, 2017: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/28230288/a-dual-label-time-resolved-fluorescence-immunoassay-for-the-simultaneous-determination-of-ferritin-and-%C3%AE-2-microglobulin
#13
Zhi Liu, Jing Huang, Rui-Ming Ou, Meng-Dong Yao, Yan-Lin She, Rui Chen, Cheng Li, Li Xu, Aikebaier Abudureyimu, Qing Zhang, Shuang Liu
BACKGROUND: Lymphocytic leukemia is a kind of primary malignant tumor of hematopoietic tissue. The aim was to establish a dual-label time-resolved fluorescence immunoassay (TRFIA) for the simultaneous determination of ferritin (FER) and β2 -microglobulin (β2 -MG) for the early screening and follow-up surveillance of lymphocytic leukemia. METHODS: The sandwich immunoassay was used to detect the concentration of FER, and the competitive immunoassay was used to detect the concentration of β2 -MG in serum...
February 23, 2017: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/28220888/mintmap-fast-and-exhaustive-profiling-of-nuclear-and-mitochondrial-trna-fragments-from-short-rna-seq-data
#14
Phillipe Loher, Aristeidis G Telonis, Isidore Rigoutsos
Transfer RNA fragments (tRFs) are an established class of constitutive regulatory molecules that arise from precursor and mature tRNAs. RNA deep sequencing (RNA-seq) has greatly facilitated the study of tRFs. However, the repeat nature of the tRNA templates and the idiosyncrasies of tRNA sequences necessitate the development and use of methodologies that differ markedly from those used to analyze RNA-seq data when studying microRNAs (miRNAs) or messenger RNAs (mRNAs). Here we present MINTmap (for MItochondrial and Nuclear TRF mapping), a method and a software package that was developed specifically for the quick, deterministic and exhaustive identification of tRFs in short RNA-seq datasets...
February 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28176266/effects-on-the-torsional-vibration-behavior-in-the-investigation-of-dental-implant-osseointegration-using-resonance-frequency-analysis-a-numerical-approach
#15
Min Zhai, Bing Li, Dehua Li
Resonance frequency analysis (RFA) methods are widely used to assess implant stability, particularly the Osstell(®) device. The potential effects associated with this method have been discussed in the literature. Torsional RFA (T-RFA), mentioned in our previous study, could represent a new measurement method. The purpose of this study was to simulate T-shaped and Osstell(®) transducer-implant-bone system models; compare their vibration modes and corresponding resonance frequencies; and investigate the effects of their parameters, such as the effective implant length (EIL), bone quality, and osseointegration level, on the torsional resonance frequency (TRF) and bending resonance frequency (BRF) using three-dimensional finite element analysis...
February 7, 2017: Medical & Biological Engineering & Computing
https://www.readbyqxmd.com/read/28167310/change-trajectories-of-aggressive-behavior-among-children-in-long-term-residential-care
#16
Shalhevet Attar-Schwartz, Rami Benbenishty, Ilan Roziner
This study examined change trajectories of aggressive behaviors among children in long-term residential care in Israel and identified various child-, family-, and placement-related predictors of the change trajectories. Records of 799 children (average age at the beginning of the four years=10.4, 33.1% female) in their first four consecutive years in care were analyzed using the TRF aggressive behavior subscale (Achenbach, 1991) to measure the outcome variable. Children's and parents' characteristics and placement-related factors were used to explain variance in change trajectories...
March 2017: Child Abuse & Neglect
https://www.readbyqxmd.com/read/28133587/non-radioactive-trf-assay-modifications-to-improve-telomeric-dna-detection-efficiency-in-plants
#17
Liliia R Nigmatullina, Margarita R Sharipova, Eugene V Shakirov
The length of telomeric DNA is often considered a cellular biomarker of aging and general health status. Several telomere length measuring assays have been developed, of which the most common is the Telomere Restriction Fragment (TRF) analysis, which typically involves the use of radioactively labeled oligonucleotide probes. While highly effective, this method potentially poses substantial health concerns and generates radioactive waste. Digoxigenin (DIG) alternatives to radioactive probes have been developed and used successfully in a number of assays...
December 2016: BioNanoScience
https://www.readbyqxmd.com/read/28129920/sex-dependent-telomere-shortening-telomerase-activity-and-oxidative-damage-in-marine-medaka-oryzias-melastigma-during-aging
#18
Bill Wp Yip, Helen Ol Mok, Drew R Peterson, Miles T Wan, Y Taniguchi, Wei Ge, Doris Wt Au
Marine medaka Oryzias melastigma at 4months (young), 8months (middle-aged) and 12months old (senior) were employed to determine age-associated change of sex ratios, sex hormones, telomere length (TL), telomerase activity (TA), telomerase transcription (omTERT) and oxidative damage in the liver. Overall, O. melastigma exhibited gradual senescence, sex differences in longevity (F>M), TL (F>M) and oxidative damage (F<M) during aging. In females, the plasma E2 level was positively correlated with TL (TRF>5kb), TA and omTERT expression (p≤0...
January 24, 2017: Marine Pollution Bulletin
https://www.readbyqxmd.com/read/28128550/ultrasensitive-sensing-material-based-on-opal-photonic-crystal-for-label-free-monitoring-of-transferrin
#19
Enqi Wu, Yuan Peng, Xihao Zhang, Jialei Bai, Yanqiu Song, Houluo He, Longxing Fan, Xiaochen Qu, Zhixian Gao, Ying Liu, Baoan Ning
A new opal photonic crystal (PC) sensing material, allowing label-free detection of transferrin (TRF), is proposed in the current study. This photonic crystal was prepared via a vertical convective self-assembly method with monodisperse microspheres polymerized by methyl methacrylate (MMA) and 3-acrylamidophenylboronic acid (AAPBA). FTIR, TG, and DLS were used to characterize the components and particle size of the monodisperse microspheres. SEM was used to observe the morphology of the PC. The diffraction peak intensity decreases as the TRF concentration increase...
February 22, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28124217/elemental-analysis-of-whole-and-protein-separated-blood-serum-of-patients-with-systemic-lupus-erythematosus-and-sj%C3%A3-gren-s-syndrome
#20
Csilla Noémi Tóth, Edina Baranyai, István Csípő, Tünde Tarr, Margit Zeher, József Posta, István Fábián
Systemic lupus erythematosus (SLE) and Sjögren's syndrome (SS) are systemic autoimmune diseases with complex symptoms and pathogenesis that are still not completely understood. Several studies showed that the trace element homeostasis and also the levels of antioxidant plasma proteins are changed in autoimmune disorders; however, these results are controversial. In this study, the potassium (K), calcium (Ca), magnesium (Mg), copper (Cu), zinc (Zn), and iron (Fe) concentrations of the serum and proteins-immunoglobulin G (IgG), transferrin (Trf), albumin (Alb), and ceruloplasmin (Cp)-separated from serum samples by affinity chromatography were determined in patients with SLE and SS...
January 26, 2017: Biological Trace Element Research
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