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https://www.readbyqxmd.com/read/29779414/the-effect-of-intrauterine-growth-on-leukocyte-telomere-length-at-birth
#1
Alketa Stefa, Agaristi Lamprokostopoulou, Despina D Briana, Anna Kontogeorgou, Ifigeneia Papageorgiou, Ariadne Malamitsi-Puchner, Ourania Tsitsilonis, Sarantis Gagos, Evangelia Charmandari
OBJECTIVE: Telomeres are specialized nucleoprotein structures located at the ends of chromosomes, which play a crucial role in genomic stability. Telomere shortening has been proposed as a biomarker for the onset of age-related diseases. This study aimed to determine whether restricted or increased intrauterine growth affects leukocyte telomere length (LTL) at birth. MATERIAL AND METHODS: One hundred sixty five (n = 165) full-term neonates participated in the study...
May 20, 2018: Journal of Maternal-fetal & Neonatal Medicine
https://www.readbyqxmd.com/read/29760179/time-restricted-feeding-prevents-ablation-of-diurnal-rhythms-in-gastric-vagal-afferent-mechanosensitivity-observed-in-high-fat-diet-induced-obese-mice
#2
Stephen J Kentish, George Hatzinikolas, Hui Li, Claudine A Frisby, Gary A Wittert, Amanda J Page
Mechanosensitive gastric vagal afferents (GVAs) are involved in the regulation of food intake. GVAs exhibit diurnal rhythmicity in their response to food related stimuli allowing time of day specific satiety signalling. This diurnal rhythmicity is ablated in high fat diet (HFD)-induced obesity. Time restricted feeding (TRF) has a strong influence on peripheral clocks. This study aimed to determine whether diurnal patterns in GVA mechanosensitivity are entrained by TRF.Eight-week old male C57BL/6 mice (N=256) were fed a standard laboratory diet (SLD) or HFD for 12wks...
May 14, 2018: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/29750639/harmonization-of-units-and-reference-intervals-of-plasma-proteins-state-of-the-art-from-an-external-quality-assessment-scheme
#3
Sandra Secchiero, Laura Sciacovelli, Mario Plebani
BACKGROUND: The need to harmonize laboratory information is particularly intense in the field of plasma proteins, considering their clinical impact and relevance in monitoring diseases. METHODS: We evaluated units and reference intervals (RIs) utilized by participants of the External Quality Assessment Scheme (EQAS) for plasma proteins of the Centre of Biomedical Research. Moreover, we evaluated inter-laboratory analytical variability from 2001 to 2017. RESULTS: The census of participants' units employed in 2017 showed that for albumin (ALB), ~66% of laboratories still used dL instead of L, and for most other proteins, ~70% still expressed the results in mg/dL...
May 11, 2018: Clinical Chemistry and Laboratory Medicine: CCLM
https://www.readbyqxmd.com/read/29748504/trna-derived-small-rna-a-novel-regulatory-small-non-coding-rna
#4
REVIEW
Siqi Li, Zhengping Xu, Jinghao Sheng
Deep analysis of next-generation sequencing data unveils numerous small non-coding RNAs with distinct functions. Recently, fragments derived from tRNA, named as tRNA-derived small RNA (tsRNA), have attracted broad attention. There are mainly two types of tsRNAs, including tRNA-derived stress-induced RNA (tiRNA) and tRNA-derived fragment (tRF), which differ in the cleavage position of the precursor or mature tRNA transcript. Emerging evidence has shown that tsRNAs are not merely tRNA degradation debris but have been recognized to play regulatory roles in many specific physiological and pathological processes...
May 10, 2018: Genes
https://www.readbyqxmd.com/read/29748193/human-angiogenin-is-a-potent-cytotoxin-in-the-absence-of-ribonuclease-inhibitor
#5
Sydney P Thomas, Trish T Hoang, Valerie T Ressler, Ronald T Raines
Angiogenin (ANG) is a secretory ribonuclease that promotes the proliferation of endothelial cells, leading to angiogenesis. This function relies on its ribonucleolytic activity, which is low for simple RNA substrates. Upon entry into the cytosol, ANG is sequestered by the ribonuclease inhibitor protein (RNH1). We find that ANG is a potent cytotoxin for RNH1 -knockout HeLa cells, belying its inefficiency as a nonspecific catalyst. The toxicity does, however, rely on the ribonucleolytic activity of ANG and a cytosolic localization, which lead to the accumulation of particular tRNA fragments (tRFs), such as tRF-5 Gly-GCC...
May 10, 2018: RNA
https://www.readbyqxmd.com/read/29717885/time-restricted-feeding-of-a-high-fat-diet-in-c57bl-6-male-mice-reduces-adiposity-but-does-not-protect-against-increased-systemic-inflammation
#6
Laura B Delahaye, Richard J Bloomer, Matthew B Butawan, Jacqueline M Wyman, Jessica L Hill, Harold W Lee, Andrew C Liu, Liam McAllan, Joan C Han, Marie van der Merwe
Time-restricted feeding (TRF) limits duration of food availability without altering diet composition and can combat obesity in humans and mice. For this study we evaluated the effect of timing of food access during a TRF protocol on weight gain, adiposity and inflammation. Young C57BL/6 male mice were placed on a 45% high fat (HF) diet for 8 weeks. Food access was unrestricted (HF) or restricted to 6 hours per day, either for the first half (HF-early) or the second half (HF-late) of the active phase to resemble a window of time for food consumption early <u><i></u>vs<u></i></u> late in the day in a human population...
May 2, 2018: Applied Physiology, Nutrition, and Metabolism, Physiologie Appliquée, Nutrition et Métabolisme
https://www.readbyqxmd.com/read/29685897/characterization-of-human-salivary-extracellular-rna-by-next-generation-sequencing
#7
Feng Li, Karolina Elżbieta Kaczor-Urbanowicz, Jie Sun, Blanca Majem, Hsien-Chun Lo, Yong Kim, Kikuye Koyano, Shannon Liu Rao, So Young Kang, Su Mi Kim, Kyoung-Mee Kim, Sung Kim, David Chia, David Elashoff, Tristan R Grogan, Xinshu Xiao, David T W Wong
BACKGROUND: It was recently discovered that abundant and stable extracellular RNA (exRNA) species exist in bodily fluids. Saliva is an emerging biofluid for biomarker development for noninvasive detection and screening of local and systemic diseases. Use of RNA-Sequencing (RNA-Seq) to profile exRNA is rapidly growing; however, no single preparation and analysis protocol can be used for all biofluids. Specifically, RNA-Seq of saliva is particularly challenging owing to high abundance of bacterial contents and low abundance of salivary exRNA...
April 23, 2018: Clinical Chemistry
https://www.readbyqxmd.com/read/29679925/tocotrienol-rich-fraction-supplementation-reduces-hyperglycemia-induced-skeletal-muscle-damage-through-regulation-of-insulin-signaling-and-oxidative-stress-in-type-2-diabetic-mice
#8
Heaji Lee, Yunsook Lim
Chronic hyperglycemia induces impairment of muscle growth and development of diabetes mellitus (DM). Since skeletal muscle is the major site for disposal of ingested glucose, impaired glucose metabolism causes imbalance between protein synthesis and degradation which adversely affects physical mobility. In this study, we investigated the effect of tocotrienol-rich fraction (TRF) supplementation on skeletal muscle damage in diabetic mice. Diabetes was induced by a high-fat diet with streptozotocin (STZ) injection (100 mg/kg) in male C57BL/6J mice...
March 21, 2018: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/29679420/a-dual-label-time-resolved-fluorescence-immunoassay-for-the-simultaneous-determination-of-carcino-embryonic-antigen-and-squamous-cell-carcinoma-antigen
#9
Qian Mao, Xiaohong Liu, Jingwen Ye, Huankun Liang, Binxian Li, Xinyi Sun, Lin Sun, Yuman Sun, Xiaomei Mu, Laiqing Li
Among women worldwide, cervical cancer is the second-most common cancer, and cervical smears and DNA detection have low sensitivity or are too expensive. The concentrations of carcino-embryonic antigen (CEA) and squamous cell carcinoma (SCC) in the serum were detected using a sandwich immunoassay. The CEA and SCC in the serum were captured by anti-CEA and anti-SCC antibodies. After combining other anti-CEA- and anti-SCC-labeled antibodies with europium (III) (Eu3+ ) and samarium (III) (Sm3+ ) chelates, CEA and SCC were detected with time-resolved fluorometry (TRF)...
April 20, 2018: Biotechnology and Applied Biochemistry
https://www.readbyqxmd.com/read/29679193/etiology-of-stability-and-growth-of-internalizing-and-externalizing-behavior-problems-across-childhood-and-adolescence
#10
Alexander S Hatoum, Soo Hyun Rhee, Robin P Corley, John K Hewitt, Naomi P Friedman
Internalizing and externalizing behaviors are heritable, and show genetic stability during childhood and adolescence. Less work has explored how genes influence individual differences in developmental trajectories. We estimated ACE biometrical latent growth curve models for the Teacher Report Form (TRF) and parent Child Behavior Checklist (CBCL) internalizing and externalizing scales from ages 7 to 16 years in 408 twin pairs from the Colorado Longitudinal Twin Study. We found that Intercept factors were highly heritable for both internalizing and externalizing behaviors (a2 = ...
April 20, 2018: Behavior Genetics
https://www.readbyqxmd.com/read/29675569/effect-of-zinc-sulfate-and-zinc-glycine-chelate-on-concentrations-of-acute-phase-proteins-in-chicken-serum-and-liver-tissue
#11
Łukasz Jarosz, Agnieszka Marek, Zbigniew Grądzki, Ewa Laskowska, Małgorzata Kwiecień
The aim of the study was to determine how inorganic and organic forms of zinc affect the concentrations of C-reactive protein (CRP), serum amyloid A (SAA), alpha-1-acid glycoprotein (α-1-AGP), haptoglobin (Hp), and transferrin (TRF) in the blood and liver tissue of 450 1-day-old Ross 308 chicken. Four experimental groups received one the following: inorganic zinc (ZnSO4 ), a zinc phytase enzyme supplement (ZnSO4 -F), organic zinc in combination with glycine (Zn-Gly), or organic zinc supplemented with phytase (Zn-Gly-F)...
April 19, 2018: Biological Trace Element Research
https://www.readbyqxmd.com/read/29674048/efficient-preparation-of-surface-imprinted-magnetic-nanoparticles-using-poly-2-anilinoethanol-as-imprinting-coating-for-the-selective-recognition-of-glycoprotein
#12
Daojin Li, Tianyong Tu, Mengke Yang, Chen Xu
In view of the significance of glycoprotein biomarkers for early clinical diagnostics and treatments of diseases, it is essential to develop efficient and selective enrichment approaches for glycoproteins. Molecularly imprinted polymers (MIPs) have found important applications for separation and enrichment of glycoproteins. In this study, we use boronate affinity-based controllable oriented surface imprinting to prepare glycoprotein-imprinted magnetic nanoparticles. A glycoprotein was first immobilized onto the surface of boronic acid functionalized magnetic nanoparticles by boronate affinity...
July 1, 2018: Talanta
https://www.readbyqxmd.com/read/29657238/the-first-report-of-terbinafine-resistance-microsporum-canis-from-a-cat
#13
Yun-Hsia Hsiao, Charles Chen, Hock Siew Han, Rui Kano
A 2-year-old, exotic shorthair cat presented with baldness and mild scaling on trunk that was confirmed as Microsporum canis (M. canis) infection by the following methods. Wood's lamp and trichogram were used to demonstrate fungal elements suggestive of dermatophytosis consistent with M. canis. Dermatophyte test medium (DTM) and polymerase chain reaction (PCR) were used for identification. E-test and broth microdilution test were then utilized to estimate antifungal minimal inhibitory concentrations (MICs) towards ITZ and TRF respectively...
April 16, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29628141/pseudouridylation-of-trna-derived-fragments-steers-translational-control-in-stem-cells
#14
Nicola Guzzi, Maciej Cieśla, Phuong Cao Thi Ngoc, Stefan Lang, Sonali Arora, Marios Dimitriou, Kristyna Pimková, Mikael N E Sommarin, Roberto Munita, Michal Lubas, Yiting Lim, Kazuki Okuyama, Shamit Soneji, Göran Karlsson, Jenny Hansson, Göran Jönsson, Anders H Lund, Mikael Sigvardsson, Eva Hellström-Lindberg, Andrew C Hsieh, Cristian Bellodi
Pseudouridylation (Ψ) is the most abundant and widespread type of RNA epigenetic modification in living organisms; however, the biological role of Ψ remains poorly understood. Here, we show that a Ψ-driven posttranscriptional program steers translation control to impact stem cell commitment during early embryogenesis. Mechanistically, the Ψ "writer" PUS7 modifies and activates a novel network of tRNA-derived small fragments (tRFs) targeting the translation initiation complex. PUS7 inactivation in embryonic stem cells impairs tRF-mediated translation regulation, leading to increased protein biosynthesis and defective germ layer specification...
April 4, 2018: Cell
https://www.readbyqxmd.com/read/29620181/identification-of-streptococcus-mitis321a-vaccine-antigens-based-on-reverse-vaccinology
#15
Qiao Zhang, Kexiong Lin, Changzheng Wang, Zhi Xu, Li Yang, Qianli Ma
Streptococcus mitis (S. mitis) may transform into highly pathogenic bacteria. The aim of the present study was to identify potential antigen targets for designing an effective vaccine against the pathogenic S. mitis321A. The genome of S. mitis321A was sequenced using an Illumina Hiseq2000 instrument. Subsequently, Glimmer 3.02 and Tandem Repeat Finder (TRF) 4.04 were used to predict genes and tandem repeats, respectively, with DNA sequence function analysis using the Basic Local Alignment Search Tool (BLAST) in the Kyoto Encyclopedia of Genes and Genomes (KEGG) and Cluster of Orthologous Groups of proteins (COG) databases...
March 28, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29589779/telomere-length-is-independently-associated-with-age-oxidative-biomarkers-and-sport-training-in-skeletal-muscle-of-healthy-adult-males
#16
Fiorenza Magi, Ivan Dimauro, Fabrizio Margheritini, Guglielmo Duranti, Neri Mercatelli, Cristina Fantini, Francesca Romana Ripani, Stefania Sabatini, Daniela Caporossi
In skeletal muscle, which mainly contains postmitotic myonuclei, it has been suggested that telomere length remains roughly constant throughout adult life, or shortens in response to physiopathological conditions in muscle diseases or in the elderly. However, telomere length results from both the replicative history of a specific tissue and the exposure to environmental, DNA damage-related factors, therefore the predictive biological significance of telomere measures should combine the analysis of the various interactive factors...
March 28, 2018: Free Radical Research
https://www.readbyqxmd.com/read/29571007/time-restricted-feeding-influences-immune-responses-without-compromising-muscle-performance-in-older-men
#17
Maha Gasmi, Maha Sellami, Joshua Denham, Johnny Padulo, Goran Kuvacic, Walid Selmi, Riadh Khalifa
OBJECTIVE: This study examined the effect of 12 wk of time-restricted feeding (TRF) on complete blood cell counts, natural killer cells, and muscle performance in 20- and 50-year-old men. METHODS: Forty active and healthy participants were randomly divided into young experimental, young control, aged experimental, and aged control group. Experimental groups participated in TRF. Before (P1) and after (P2) TRF, participants performed a maximal exercise test to quantify muscle power...
February 3, 2018: Nutrition
https://www.readbyqxmd.com/read/29561610/unveiling-the-potential-of-unfused-bichromophoric-naphthalimide-to-induce-cytotoxicity-by-binding-to-tubulin-breaks-monotony-of-naphthalimides-as-conventional-intercalators
#18
Ritika S Joshi, Dipanwita Das Mukherjee, Subhendu Chakrabarty, Ansie Martin, Manojkumar Jadhao, Gopal Chakrabarti, Angshuman Sarkar, Sujit Kumar Ghosh
In the development of small molecule drug candidates, naphthalimide based compounds hold a very important position as potent anti-cancer agents with considerable safety in drug discoveries. Being synthetically and readily accessible, naphthalimide compounds with planar architecture have been developed mostly as DNA targeting intercalators. However, in this article, we demonstrate, for the first time, wherein an unfused naphthalimide-benzothiazole bichromophoric compound 2-(6-chlorobenzo[d] thiazol-2-yl)-1H-benzo[de] isoquinoline-1,3(2H)-dione (CBIQD), designed and synthesized by our group, seem to expand the bioactivity of naphthalimide as anti-mitotic agents also...
March 21, 2018: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/29561243/a-stable-trna-like-molecule-is-generated-from-the-long-noncoding-rna-gut15-in-arabidopsis
#19
Patrycja Plewka, Agnieszka Thompson, Maciej Szymanski, Przemyslaw Nuc, Katarzyna Knop, Agnieszka Rasinska, Aleksandra Bialkowska, Zofia Szweykowska-Kulinska, Wojciech M Karlowski, Artur Jarmolowski
The Arabidopsis GUT15 RNA belongs to a class of noncoding RNAs that are expressed from the intergenic regions of protein-coding genes. We show that the RNA polymerase II transcribed GUT15 transcript serves as a precursor for two stable RNA species, a tRNA-like molecule and GUT15-tRF-F5, which are both encoded by the final intron in the GUT15 gene. The GUT15-encoded tRNA-like molecule cannot be autonomously transcribed by RNA polymerase III. However, this molecule contains a CCA motif, suggesting that it may enter the tRNA maturation pathway...
March 21, 2018: RNA Biology
https://www.readbyqxmd.com/read/29542055/use-of-u-stela-for-accurate-measurement-of-extremely-short-telomeres
#20
Nedime Serakinci, Huseyin Cagsin, Merdiye Mavis
Telomeres are repetitive genetic materials that protect the chromosomes by capping the ends of chromosomes. Each time a cell divides, telomeres get shorter. Telomere length is mainly maintained by telomerase. This enzyme is present in the embryonic stem cells in high concentrations and declines with age. It is still unclear to what extend there is telomerase in adult stem cells, but considering these are the founder cells to the cells of the all tissues in a body, understanding the telomere dynamics and expression of telomerase in adult stem cells is very important...
March 15, 2018: Methods in Molecular Biology
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