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https://www.readbyqxmd.com/read/28738579/compositional-engineering-of-vopcpho-tio2-nano-composite-to-reduce-the-absolute-threshold-value-of-humidity-sensors
#1
Mohamad Izzat Azmer, Fakhra Aziz, Zubair Ahmad, Ehsan Raza, Mansoor Ani Najeeb, Noshin Fatima, Tahani M Bawazeer, Mohammad S Alsoufi, R A Shakoor, Khaulah Sulaiman
This research work demonstrates compositional engineering of an organic-inorganic hybrid nano-composites for modifying absolute threshold of humidity sensors. Vanadyl-2,9,16,23-tetraphenoxy-29H,31H-phthalocyanine (VOPcPhO), an organic semiconductor, doped with Titanium-dioxide nanoparticles (TiO2 NPs) has been employed to fabricate humidity sensors. The morphology of the VOPcPhO:TiO2 nano-composite films has been analyzed by atomic force microscopy (AFM) and field emission scanning electron microscopy (FESEM)...
November 1, 2017: Talanta
https://www.readbyqxmd.com/read/28738504/prevention-of-melanin-formation-during-aryl-alcohol-oxidase-production-under-growth-limited-conditions-using-an-aspergillus-nidulans-cell-factory
#2
Oscar Pardo-Planas, Rolf A Prade, Michael Müller, Hasan K Atiyeh, Mark R Wilkins
An Aspergillus nidulans cell factory was genetically engineered to produce an aryl alcohol oxidase (AAO). The cell factory initiated production of melanin when growth-limited conditions were established using stationary plates and shaken flasks. This phenomenon was more pronounced when the strain was cultured in a trickle bed reactor (TBR). This study investigated different approaches to reduce melanin formation in fungal mycelia and liquid medium in order to increase the enzyme production yield. Removal of copper from the medium recipe reduced melanin formation in agar cultures and increased enzyme activities by 48% in agitated liquid cultures...
July 8, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28738282/fouling-behavior-of-silica-nanoparticle-surfactant-mixtures-during-constant-flux-dead-end-ultrafiltration
#3
Krzysztof W Trzaskus, Sooi Li Lee, Wiebe M de Vos, Antoine Kemperman, Kitty Nijmeijer
The increasing use of engineered nanoparticles in customer products results in their accumulation in water sources. In this experimental study, we investigated the role of surfactant type (cationic, anionic and non-ionic) and concentration on fouling development, nanoparticle rejection and fouling irreversibility during dead-end ultrafiltration of model silica nanoparticles. Our work demonstrates that the type of surfactant influences the nanoparticle stability, which in turn is responsible for differences in fouling behavior of the nanoparticles...
July 15, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28738232/molecular-tools-and-emerging-strategies-for-deep-genetic-genomic-refactoring-of-pseudomonas
#4
REVIEW
Esteban Martínez-García, Víctor de Lorenzo
The interest of the genus Pseudomonas largely relies on the virulence of some of its species for plants and animals (including humans). Yet, pathogenic features of some isolates coexist with others often present in environmental variants that promote plant growth and degrade chemical pollutants. Many of these traits can be traced to the intrinsic properties of the genomic chassis of this genus along with distinct genetic parts and devices. With the tools of Synthetic Biology these can be enhanced and/or repurposed for the sake of biological control, environmental remediation and whole-cell biocatalysis...
July 21, 2017: Current Opinion in Biotechnology
https://www.readbyqxmd.com/read/28738223/engineering-resistance-against-viroids
#5
REVIEW
Ricardo Flores, Beatriz Navarro, Natalia Kovalskaya, Rosemarie W Hammond, Francesco Di Serio
Viroids, the smallest infectious agents endowed with autonomous replication, are tiny single-stranded circular RNAs (∼250 to 400nt) without protein-coding ability that, despite their simplicity, infect and often cause disease in herbaceous and woody plants of economic relevance. To mitigate the resulting losses, several strategies have been developed, the most effective of which include: firstly, search for naturally resistant cultivars and breeding for resistance, secondly, induced resistance by pre-infection with mild strains, thirdly, ribonucleases targeting double-stranded RNAs and catalytic antibodies endowed with intrinsic ribonuclease activity, fourthly, antisense, and sense, RNAs, fifthly, catalytic antisense RNAs derived from hammerhead ribozymes, and sixthly, hairpin RNAs and artificial small RNAs for RNA interference...
July 21, 2017: Current Opinion in Virology
https://www.readbyqxmd.com/read/28738208/plastid-transformation-and-its-application-in-metabolic-engineering
#6
REVIEW
Paulina Fuentes, Tegan Armarego-Marriott, Ralph Bock
Metabolic pathway engineering by transgene expression from the plastid (chloroplast) genome offers significant attractions, including straightforward multigene engineering by pathway expression from operons, high transgene expression levels, and increased transgene containment due to maternal inheritance of plastids in most crops. In addition, it provides direct access to the large and diverse metabolite pools in chloroplasts and non-green plastid types. Here, we review recent progress with extending the toolbox for plastid engineering and highlight selected applications in the area of metabolic engineering, including the combined engineering of nuclear and plastid genomes for the production of artemisinic acid, the direct harness of chloroplast reducing power for the synthesis of dhurrin and the use of an edible host for the production of astaxanthin...
July 21, 2017: Current Opinion in Biotechnology
https://www.readbyqxmd.com/read/28738155/engineering-aromatic-aromatic-interactions-to-nucleate-folding-in-intrinsically-disordered-regions-of-proteins
#7
Swati Balakrishnan, Siddhartha Peddibhotla Sarma
Aromatic interactions are known to be an important force in protein folding as they combine the stability of a hydrophobic interaction with the selectivity of a hydrogen bond. Much of our understanding of aromatic interactions comes from analyses of protein structures and from the contribution of these interactions to stabilizing secondary structure motifs in model peptides. In this study the structural consequences of aromatic interactions on protein folding have been explored in engineered mutants of the molten globule protein apo - cytochrome b5...
July 24, 2017: Biochemistry
https://www.readbyqxmd.com/read/28737932/ins-and-outs-in-environmental-and-occupational-safety-studies-of-asthma-and-engineered-nanomaterials
#8
Marina A Dobrovolskaia, Michael R Shurin, Valerian E Kagan, Anna A Shvedova
According to the Centers for Disease Control and Prevention, approximately 25 million Americans suffer from asthma. The disease total annual cost is about $56 billion and includes both the direct and indirect costs of medications, hospital stays, missed work, and decreased productivity. Air pollution with xenobiotics, bacterial agents, and industrial nanomaterials, such as carbon nanotubes, contribute to the exacerbation of this condition and are a point of particular attention in environmental toxicology as well as in occupational health and safety research...
July 24, 2017: ACS Nano
https://www.readbyqxmd.com/read/28737822/liquid-crystal-templating-as-an-approach-to-spatially-and-temporally-organise-soft-matter
#10
REVIEW
Pim van der Asdonk, Paul H J Kouwer
Chemistry quickly moves from a molecular science to a systems science. This requires spatial and temporal control over the organisation of molecules and molecular assemblies. Whilst Nature almost by default (transiently) organises her components at multiple different length scales, scientists struggle to realise even relatively straightforward patterns. In the past decades, supramolecular chemistry has taught us the rules to precisely engineer molecular assembly at the nanometre scale. At higher length scales, however, we are bound to top-down nanotechnology techniques to realise order...
July 24, 2017: Chemical Society Reviews
https://www.readbyqxmd.com/read/28737747/a-spatially-localized-architecture-for-fast-and-modular-dna-computing
#11
Gourab Chatterjee, Neil Dalchau, Richard A Muscat, Andrew Phillips, Georg Seelig
Cells use spatial constraints to control and accelerate the flow of information in enzyme cascades and signalling networks. Synthetic silicon-based circuitry similarly relies on spatial constraints to process information. Here, we show that spatial organization can be a similarly powerful design principle for overcoming limitations of speed and modularity in engineered molecular circuits. We create logic gates and signal transmission lines by spatially arranging reactive DNA hairpins on a DNA origami. Signal propagation is demonstrated across transmission lines of different lengths and orientations and logic gates are modularly combined into circuits that establish the universality of our approach...
July 24, 2017: Nature Nanotechnology
https://www.readbyqxmd.com/read/28737744/delivering-advanced-therapies-the-big-pharma-approach
#12
REVIEW
J Tarnowski, D Krishna, L Jespers, A Ketkar, R Haddock, J Imrie, S Kili
After two decades of focused development and some recent clinical successes, cell and gene therapy (CGT) is emerging as a promising approach to personalized medicines. Genetically engineered cells as a medical modality are poised to stand alongside or in combination with small molecule and biopharmaceutical approaches to bring new therapies to patients globally. Big pharma can play a vital role in industrializing CGT by focusing on diseases with high un-met medical need and compelling genetic evidence. Pharma should invest in manufacturing and supply chain solutions that deliver reproducible, high quality therapies at a commercially viable cost...
July 24, 2017: Gene Therapy
https://www.readbyqxmd.com/read/28737741/rapidly-inducible-cas9-and-dsb-ddpcr-to-probe-editing-kinetics
#13
John C Rose, Jason J Stephany, William J Valente, Bridget M Trevillian, Ha V Dang, Jason H Bielas, Dustin J Maly, Douglas M Fowler
We developed a chemically inducible Cas9 (ciCas9) and a droplet digital PCR assay for double-strand breaks (DSB-ddPCR) to investigate the kinetics of Cas9-mediated generation and repair of DSBs in cells. ciCas9 is a rapidly activated, single-component Cas9 variant engineered by replacing the protein's REC2 domain with the BCL-xL protein and fusing an interacting BH3 peptide to the C terminus. ciCas9 can be tunably activated by a compound that disrupts the BCL-xL-BH3 interaction within minutes. DSB-ddPCR demonstrates time-resolved, highly quantitative, and targeted measurement of DSBs...
July 24, 2017: Nature Methods
https://www.readbyqxmd.com/read/28737736/tomato-development-mads-box-engineering
#14
Jun Lyu
No abstract text is available yet for this article.
July 24, 2017: Nature Plants
https://www.readbyqxmd.com/read/28737701/application-of-extrusion-based-hydrogel-bioprinting-for-cartilage-tissue-engineering
#15
REVIEW
Fu You, B Frank Eames, Xiongbiao Chen
Extrusion-based bioprinting (EBB) is a rapidly developing technique that has made substantial progress in the fabrication of constructs for cartilage tissue engineering (CTE) over the past decade. With this technique, cell-laden hydrogels or bio-inks have been extruded onto printing stages, layer-by-layer, to form three-dimensional (3D) constructs with varying sizes, shapes, and resolutions. This paper reviews the cell sources and hydrogels that can be used for bio-ink formulations in CTE application. Additionally, this paper discusses the important properties of bio-inks to be applied in the EBB technique, including biocompatibility, printability, as well as mechanical properties...
July 23, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28737510/lymphocytes-transiently-expressing-virus-specific-t-cell-receptors-reduce-hepatitis-b-virus-infection
#16
Janine Kah, Sarene Koh, Tassilo Volz, Erica Ceccarello, Lena Allweiss, Marc Lütgehetmann, Antonio Bertoletti, Maura Dandri
Adoptive transfer of T cells engineered to express a hepatitis B virus-specific (HBV-specific) T cell receptor (TCR) may supplement HBV-specific immune responses in chronic HBV patients and facilitate HBV control. However, the risk of triggering unrestrained proliferation of permanently engineered T cells raises safety concerns that have hampered testing of this approach in patients. The aim of the present study was to generate T cells that transiently express HBV-specific TCRs using mRNA electroporation and to assess their antiviral and pathogenetic activity in vitro and in HBV-infected human liver chimeric mice...
July 24, 2017: Journal of Clinical Investigation
https://www.readbyqxmd.com/read/28737499/tunable-indirect-direct-transition-of-few-layer-snse-via-interface-engineering
#17
Hansika Iroshini Sirikumara, Thushari Jayasekera
Tin Selenide (SnSe) is one of the best thermoelectric materials reported to date. The possibility of growing few-layer SnSe helped boost the interest in this long-known, earth abundant material. Pristine SnSe in bulk, mono- and few-layer forms are reported to have indirect electronic bandgaps. Possible indirect-direct transition in SnSe is attractive for its optoelectronic-related applications. Based on the results from first principles Density Functional Theory (DFT) calculations, we carefully analyzed electronic band structures of bulk, and bilayer SnSe with various interlayer stackings...
July 24, 2017: Journal of Physics. Condensed Matter: An Institute of Physics Journal
https://www.readbyqxmd.com/read/28737465/the-effect-of-ozone-gas-sterilization-on-the-properties-and-cell-compatibility-of-electrospun-polycaprolactone-scaffolds
#18
Carolina Fracalossi Rediguieri, Paul A De Bank, Maria Helena Ambrosio Zanin, Patrícia Leo, Natalia Neto Pereira Cerize, Adriano Marim de Oliveira, Terezinha de Jesus Andreoli Pinto
The growing area of tissue engineering has the potential to alleviate the shortage of tissues and organs for transplantation, and electrospun biomaterial scaffolds are extremely promising devices for translating engineered tissues into a clinical setting. However, to be utilized in this capacity, these medical devices need to be sterile. Traditional methods of sterilization are not always suitable for biomaterials, especially as many commonly used biomedical polymers are sensitive to chemical-, thermal- or radiation-induced damage...
July 24, 2017: Journal of Biomaterials Science. Polymer Edition
https://www.readbyqxmd.com/read/28737385/a-synthetic-circuit-for-mercury-bioremediation-using-self-assembling-functional-amyloids
#19
Pei Kun Richie Tay, Peter Q Nguyen, Neel S Joshi
Synthetic biology approaches to bioremediation are a key sustainable strategy to leverage the self-replicating and programmable aspects of biology for environmental stewardship. The increasing spread of anthropogenic mercury pollution into our habitats and food chains is a pressing concern. Here, we explore the use of programmed bacterial biofilms to aid in the sequestration of mercury. We demonstrate that by integrating a mercury-responsive promoter and an operon encoding a mercury-absorbing self-assembling extracellular protein nanofiber, we can engineer bacteria that can detect and sequester toxic Hg2+ ions from the environment...
July 24, 2017: ACS Synthetic Biology
https://www.readbyqxmd.com/read/28737278/multicellular-vascularized-engineered-tissues-through-user-programmable-biomaterial-photodegradation
#20
Christopher K Arakawa, Barry A Badeau, Ying Zheng, Cole A DeForest
A photodegradable material-based approach to generate endothelialized 3D vascular networks within cell-laden hydrogel biomaterials is introduced. Exploiting multiphoton lithography, microchannel networks spanning nearly all size scales of native human vasculature are readily generated with unprecedented user-defined 4D control. Intraluminal channel architectures of synthetic vessels are fully customizable, providing new opportunities for next-generation microfluidics and directed cell function.
July 24, 2017: Advanced Materials
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