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https://www.readbyqxmd.com/read/27933079/small-rnas-from-bemisia-tabaci-are-transferred-to-solanum-lycopersicum-phloem-during-feeding
#1
Paula J M van Kleeff, Marc Galland, Robert C Schuurink, Petra M Bleeker
The phloem-feeding whitefly Bemisia tabaci is a serious pest to a broad range of host plants, including many economically important crops such as tomato. These insects serve as a vector for various devastating plant viruses. It is known that whiteflies are capable of manipulating host-defense responses, potentially mediated by effector molecules in the whitefly saliva. We hypothesized that, beside putative effector proteins, small RNAs (sRNA) are delivered by B. tabaci into the phloem, where they may play a role in manipulating host plant defenses...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27932958/the-shark-alar-hypothalamus-molecular-characterization-of-prosomeric-subdivisions-and-evolutionary-trends
#2
Gabriel N Santos-Durán, Susana Ferreiro-Galve, Arnaud Menuet, Idoia Quintana-Urzainqui, Sylvie Mazan, Isabel Rodríguez-Moldes, Eva Candal
The hypothalamus is an important physiologic center of the vertebrate brain involved in the elaboration of individual and species survival responses. To better understand the ancestral organization of the alar hypothalamus we revisit previous data on ScOtp, ScDlx2/5, ScTbr1, ScNkx2.1 expression and Pax6 immunoreactivity jointly with new data on ScNeurog2, ScLhx9, ScLhx5, and ScNkx2.8 expression, in addition to immunoreactivity to serotonin (5-HT) and doublecortin (DCX) in the catshark Scyliorhinus canicula, a key species for this purpose since cartilaginous fishes are basal representatives of gnathostomes (jawed vertebrates)...
2016: Frontiers in Neuroanatomy
https://www.readbyqxmd.com/read/27931231/characterization-of-a-frozen-shoulder-model-using-immobilization-in-rats
#3
Du Hwan Kim, Kil-Ho Lee, Yun-Mee Lho, Eunyoung Ha, Ilseon Hwang, Kwang-Soon Song, Chul-Hyun Cho
BACKGROUND: The objective of this study was to investigate serial changes for histology of joint capsule and range of motion of the glenohumeral joint after immobilization in rats. We hypothesized that a rat shoulder contracture model using immobilization would be capable of producing effects on the glenohumeral joint similar to those seen in patients with frozen shoulder. METHODS: Sixty-four Sprague-Dawley rats were randomly divided into one control group (n = 8) and seven immobilization groups (n = 8 per group) that were immobilized with molding plaster for 3 days, or for 1, 2, 3, 4, 5, or 6 weeks...
December 8, 2016: Journal of Orthopaedic Surgery and Research
https://www.readbyqxmd.com/read/27930838/fermentation-profile-and-identification-of-lactic-acid-bacteria-and-yeasts-of-rehydrated-corn-kernel-silage
#4
B F Carvalho, C L S Ávila, T F Bernardes, M N Pereira, C Santos, R F Schwan
AIMS: The aim of this study was to evaluate the chemical and microbiological characteristics and to identify lactic acid bacteria (LAB) and yeasts involved in rehydrated corn kernel silage. METHODS AND RESULTS: Four replicates for each fermentation time: 5, 15, 30, 60, 90, 150, 210, and 280 days were prepared. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF-MS) and PCR-based identification were utilized to identify LAB and yeasts...
December 8, 2016: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/27929694/dna-methylation-levels-associate-with-race-and-childhood-asthma-severity
#5
Marcia A Chan, Christina E Ciaccio, Nicole M Gigliotti, Mo Rezaiekhaligh, Jacob A Siedlik, Kevin Kennedy, Charles S Barnes
OBJECTIVE: Asthma is a common chronic childhood disease worldwide. Socioeconomic status, genetic predisposition and environmental factors contribute to its incidence and severity. A disproportionate number of children with asthma are economically disadvantaged and live in substandard housing with potential indoor environmental exposures such as cockroaches, dust mites, rodents and molds. These exposures may manifest through epigenetic mechanisms that can lead to changes in relevant gene expression...
December 8, 2016: Journal of Asthma: Official Journal of the Association for the Care of Asthma
https://www.readbyqxmd.com/read/27929608/an-open-trial-of-mindfulness-based-stress-reduction-for-young-adults-with%C3%A2-social-anxiety-disorder
#6
Aslak Hjeltnes, Helge Molde, Elisabeth Schanche, Jon Vøllestad, Julie Lillebostad Svendsen, Christian Moltu, Per-Einar Binder
The present study investigated mindfulness-based stress reduction (MBSR) for young adults with a social anxiety disorder (SAD) in an open trial. Fifty-three young adults in a higher education setting underwent a standard eight-week MBSR program. Eight participants (15%) did not complete the program. Participants reported significant reductions in SAD symptoms and global psychological distress, as well as increases in mindfulness, self-compassion, and self-esteem. Using intention-to-treat (ITT) analyses, effect sizes ranged from large to moderate for SAD symptoms (Cohen's d = 0...
December 8, 2016: Scandinavian Journal of Psychology
https://www.readbyqxmd.com/read/27927285/in%C3%A2-vitro-effects-of-dental-cements-on-hard-and-soft-tissues-associated-with-dental-implants
#7
Lucas C Rodriguez, Juliana N Saba, Kwok-Hung Chung, Chandur Wadhwani, Danieli C Rodrigues
STATEMENT OF PROBLEM: Dental cements for cement-retained restorations are often chosen based on clinician preference for the product's material properties, mixing process, delivery mechanism, or viscosity. The composition of dental cement may play a significant role in the proliferation or inhibition of different bacterial strains associated with periimplant disease, and the effect of dental cements on host cellular proliferation may provide further insight into appropriate cement material selection...
December 4, 2016: Journal of Prosthetic Dentistry
https://www.readbyqxmd.com/read/27925656/mold-and-dampness-exposure-and-allergic-outcomes-from-birth-to-adolescence-data-from-the-bamse-cohort
#8
Jesse D Thacher, Olena Gruzieva, Göran Pershagen, Erik Melén, Johnny C Lorentzen, Inger Kull, Anna Bergström
BACKGROUND: Exposure to moldy or damp indoor environments is associated with allergic disease in young children, but it is unclear if the effects persist to adolescence. Our objective was to assess if exposure to mold or dampness during infancy increases the risk of asthma, rhinitis, or IgE sensitization in children followed from birth to 16 years of age. METHODS: We collected questionnaire derived reports of mold or dampness indicators and allergic outcomes from 3798 children in a Swedish birth cohort (BAMSE)...
December 7, 2016: Allergy
https://www.readbyqxmd.com/read/27925228/a-mock-circulatory-system-incorporating-a-compliant-3d-printed-anatomical-model-to-investigate-pulmonary-hemodynamics
#9
Paul G M Knoops, Giovanni Biglino, Alun D Hughes, Kim H Parker, Linzhang Xu, Silvia Schievano, Ryo Torii
A realistic mock circulatory system (MCS) could be a valuable in vitro testbed to study human circulatory hemodynamics. The objective of this study was to design a MCS replicating the pulmonary arterial circulation, incorporating an anatomically representative arterial model suitable for testing clinically relevant scenarios. A second objective of the study was to ensure the system's compatibility with magnetic resonance imaging (MRI) for additional measurements. A latex pulmonary arterial model with two generations of bifurcations was manufactured starting from a 3D-printed mold reconstructed from patient data...
December 7, 2016: Artificial Organs
https://www.readbyqxmd.com/read/27925030/production-of-crispy-bread-snacks-containing-chicken-meat-and-chicken-meat-powder
#10
Hulya Cakmak, Burak Altinel, Seher Kumcuoglu, Duygu Kisla, Sebnem Tavman
Chicken meat in two different forms (chicken meat and chicken meat powder) were added into white flour and whole wheat blend baguette bread formulations for protein enrichment and finally developing new and healthy snacks. The chicken meat and powder levels were 10% for white flour baguette, and 15% for whole wheat blend. The dried baguette samples were packaged under 100% N2, and physical, chemical, microbiological and sensorial properties were evaluated during 3 months of storage. Protein content of chicken meat powder added samples were found statistically higher than chicken meat added samples...
December 1, 2016: Anais da Academia Brasileira de Ciências
https://www.readbyqxmd.com/read/27924719/quantification-of-obeche-wood-allergen-development-of-a-sensitive-sandwich-elisa-for-occupational-exposure-assessment
#11
S Kespohl, P Campo, E Zahradnik, S Maryska, A Aranda-Guerrero, J Rodriguez, T Brüning, M Raulf
Obeche wood is a prominent cause of allergic occupational asthma. To reduce the risk of immunoglobulin E (IgE)-mediated sensitization it is important to assess airborne obeche wood allergen concentrations at exposed workplaces. Therefore, a highly sensitive obeche wood allergen immunoassay was developed and applicability was proven on airborne passive dust samples in Spanish wood workshops. Obeche wood sandwich enzyme-linked immunosorbent assay (ELISA) polyclonal antibodies (pAbs) were developed. Test specificity was verified by different wood and mold extracts...
2016: Journal of Toxicology and Environmental Health. Part A
https://www.readbyqxmd.com/read/27924549/identification-of-ochratoxin-a-producing-black-aspergilli-from-grapes-using-loop-mediated-isothermal-amplification-lamp-assays
#12
Michelangelo Storari, Giovanni A L Broggini
The loop-mediated isothermal amplification (LAMP) allows the rapid and specific amplification of target DNA under isothermal conditions without a prior DNA purification step. Moreover, successful amplifications can be directly evaluated through a color change of the reaction solutions. Here, we describe two LAMP assays for the detection of ochratoxin-A producing black aspergilli isolated from grapes. The two assays can detect DNA of OTA-producing black aspergilli following a very simple sample preparation and have the potential to significantly speed up the routine monitoring of these toxigenic molds in vineyards...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27924541/targeting-other-mycotoxin-biosynthetic-genes
#13
María J Andrade, Mar Rodríguez, Juan J Córdoba, Alicia Rodríguez
Real-time PCR (qPCR) methods are adequate tools for sensitive and rapid detection and quantification of toxigenic molds contaminating food commodities. Methods of qPCR for quantifying zearalenone (ZEA)-, sterigmatocystin (ST)-, cyclopiazonic acid (CPA)-, and patulin (PAT)-producing molds have been designed on the basis of specific target genes involved in the biosynthesis of these mycotoxins. In this chapter reliable qPCR protocols to detect and quantify such toxigenic molds are described. All of these methods are suitable when working with mold pure cultures and mold contaminated foods...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27924539/targeting-ochratoxin-biosynthetic-genes
#14
Antonia Gallo, Giancarlo Perrone
The pathway of ochratoxin A (OTA) biosynthesis has not yet been completely elucidated. Essentially, two kind of genes have been demonstrated to be involved in the biosynthesis of OTA. One of them is the nrps gene encoding a non-ribosomal peptide synthetase (NRPS) which catalyzes the ligation between the isocoumarin group, constituting the polyketide group of OTA molecule, and the amino acid phenylalanine.Here we describe a conventional PCR method developed for the detection of OTA-producing molds belonging to Penicillium and Aspergillus genera by Luque et al...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27924457/comparison-of-methods-to-evaluate-the-fungal-biomass-in-heating-ventilation-and-air-conditioning-hvac-dust
#15
Marie-Jeanne Biyeyeme Bi Mve, Yves Cloutier, Nancy Lacombe, Jacques Lavoie, Maximilien Debia, Geneviève Marchand
Heating, ventilation, and air-conditioning (HVAC) systems contain dust that can be contaminated with fungal spores (molds), which may have harmful effects on the respiratory health of the occupants of a building. HVAC cleaning is often based on visual inspection of the quantity of dust, without taking the mold content into account. The purpose of this study is to propose a method to estimate fungal contamination of dust in HVAC systems. Comparisons of different analytical methods were carried out on dust deposited in a controlled-atmosphere exposure chamber...
December 2016: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/27922886/bioengineered-renal-cell-therapy-device-for-clinical-translation
#16
Christopher J Pino, Angela J Westover, Deborah A Buffington, H David Humes
The Bioartificial Renal Epithelial Cell System (BRECS), is a cell-based device to treat acute kidney injury through renal cell therapy from an extracorporeal circuit. To enable widespread implementation of cell therapy, the BRECS was designed to be cryopreserved as a complete device, cryostored, cryoshipped to an end-use site, thawed as a complete device, and employed in a therapeutic extracorporeal hemofiltration circuit. This strategy overcomes storage and distribution issues that have been previous barriers to cell therapy...
December 2, 2016: ASAIO Journal: a Peer-reviewed Journal of the American Society for Artificial Internal Organs
https://www.readbyqxmd.com/read/27917820/a-simple-method-for-producing-multiple-copies-of-controlled-release-small-molecule-microarrays-for-cell-based-screening
#17
Satoshi Fujita, Reiko Onuki-Nagasaki, Kenjiro Ikuta, Yusuke Hara
Traditional drug discovery involves the screening of lead compounds from a chemical library by using cell-based high throughput screening (HTS) procedures. This has created a demand for the development of cell-based microarray chips for HTS of compounds. Although several cell-based microarray devices and procedures for screening of chemical libraries have been reported, each has limitations in terms of simplicity, speed, and cost. Here, we sought to make a simple method for producing multiple copies of microarray chips for the controlled release of small molecules during cell-based screening...
December 5, 2016: Biofabrication
https://www.readbyqxmd.com/read/27917252/separation-of-superparamagnetic-particles-through-ratcheted-brownian-motion-and-periodically-switching-magnetic-fields
#18
Fan Liu, Li Jiang, Huei Ming Tan, Ashutosh Yadav, Preetika Biswas, Johan R C van der Maarel, Christian A Nijhuis, Jeroen A van Kan
Brownian ratchet based particle separation systems for application in lab on chip devices have drawn interest and are subject to ongoing theoretical and experimental investigations. We demonstrate a compact microfluidic particle separation chip, which implements an extended on-off Brownian ratchet scheme that actively separates and sorts particles using periodically switching magnetic fields, asymmetric sawtooth channel sidewalls, and Brownian motion. The microfluidic chip was made with Polydimethylsiloxane (PDMS) soft lithography of SU-8 molds, which in turn was fabricated using Proton Beam Writing...
November 2016: Biomicrofluidics
https://www.readbyqxmd.com/read/27916331/modifications-to-the-fenestration-approach-for-arytenoid-adduction-under-local-anesthesia
#19
Takahiro Fukuhara, Tsuyoshi Morisaki, Hideyuki Kataoka, Naritomo Miyake, Kenkichiro Taira, Satoshi Koyama, Kazunori Fujiwara, Hiroya Kitano, Hiromi Takeuchi
OBJECTIVE: We modified the fenestration approach for arytenoid adduction to make it easier to perform the surgery. The aim of this study was to evaluate the usefulness of our modifications, which included (1) use of an Alexis wound retractor (Applied Medical) to secure the surgical field through a small incision, and (2) use of a 12-mm, 1/2 R, insert-molded taper needle with 3-0 nylon suture to prevent damage to the arytenoid cartilage. STUDY DESIGN: This is a retrospective non-randomized observational cross-sectional study...
December 1, 2016: Journal of Voice: Official Journal of the Voice Foundation
https://www.readbyqxmd.com/read/27914669/effect-of-different-thermo-light-polymerization-on-flexural-strength-of-two-glass-ionomer-cements-and-a-glass-carbomer%C3%A2-cement
#20
Kristina Gorseta, Ali Borzabadi-Farahani, Alireza Moshaverinia, Domagoj Glavina, Edward Lynch
STATEMENT OF PROBLEM: Whether polymerization lights can be used for heating glass ionomer cements (GICs) or glass carbomer (GCP) to improve their mechanical properties is not well established. PURPOSE: The purpose of this in vitro study was to assess the effect of thermo-light polymerization on the flexural strength (FS) of 2 GICs (Fuji IX GP Fast, Ketac Molar) and a GCP. MATERIAL AND METHODS: Specimens (n=10) were prepared in stainless steel molds (2×2×25 mm), compressed, exposed to 3 polymerization lights (500, 1000, 1200 mW/cm(2)) for 2 cycles of 40 seconds on each side, and stored in petroleum jelly (37°C, 24 hours)...
November 30, 2016: Journal of Prosthetic Dentistry
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