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https://www.readbyqxmd.com/read/28330314/bioremediation-of-heavy-metals-using-an-endophytic-bacterium-paenibacillus-sp-rm-isolated-from-the-roots-of-tridax-procumbens
#1
M Govarthanan, R Mythili, T Selvankumar, S Kamala-Kannan, A Rajasekar, Young-Cheol Chang
The aim of the present study was to assess the bioremediation potential of endophytic bacteria isolated from roots of Tridax procumbens plant. Five bacterial endophytes were isolated and subsequently tested for minimal inhibitory concentration (MIC) against different heavy metals. Amongst the five isolates, strain RM exhibited the highest resistance to copper (750 mg/l), followed by zinc (500 mg/l), lead (450 mg/l), and arsenic (400 mg/l). Phylogenetic analysis of the 16S rDNA sequence suggested that strain RM was a member of genus Paneibacillus...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330273/purification-and-characterization-of-polygalacturonase-from-aspergillus-fumigatus-mtcc-2584-and-elucidating-its-application-in-retting-of-crotalaria-juncea-fiber
#2
Gautam Anand, Sangeeta Yadav, Dinesh Yadav
Polygalacturonases represents an important member of pectinases group of enzymes with diverse industrial applications and is widely distributed among fungi, bacteria, yeasts, plants and some plant parasitic nematodes. An endo-polygalacturonase from a new fungal source Aspergillus fumigatus MTCC 2584 was produced under solid-state fermentation conditions and was purified simply by acetone precipitation and gel-filtration chromatography technique. The approximate molecular weight of the purified PG was found to be 43...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330267/treatment-of-landfill-leachate-using-asbr-combined-with-zeolite-adsorption-technology
#3
Chi Kim Lim, Ta Wee Seow, Chin Hong Neoh, Muhamad Hanif Md Nor, Zaharah Ibrahim, Ismail Ware, Siti Hajar Mat Sarip
Sanitary landfilling is the most common way to dispose solid urban waste; however, improper landfill management may pose serious environmental threats through discharge of high strength polluted wastewater also known as leachate. The treatment of landfill leachate to fully reduce the negative impact on the environment, is nowadays a challenge. In this study, an aerobic sequencing batch reactor (ASBR) was proposed for the treatment of locally obtained real landfill leachate with initial ammoniacal nitrogen and chemical oxygen demand (COD) concentration of 1800 and 3200 mg/L, respectively...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330266/biosorption-of-lead-copper-and-cadmium-using-the-extracellular-polysaccharides-eps-of-bacillus-sp-from-solar-salterns
#4
Syed Shameer
Extracellular Polysaccharides (EPS) from both prokaryotes and eukaryotes have a great deal of research interest as they protect the producer from different stresses including antibiotics, ionic stress, desiccation and assist in bio-film formation, pathogenesis, adhesion, etc. In this study haloalkaliphilic Bacillus sp., known to cope with osmophilic stress, was selected and screened for EPS production. The EPS were isolated, partially purified and chemical characteristics were documented using liquid FT-IR followed by assessment of heavy metal biosorption (lead, copper and cadmium) using Atomic Absorption Spectroscopy (AAS)...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330261/purification-and-characterization-of-laccase-from-marasmius-species-bbkav79-and-effective-decolorization-of-selected-textile-dyes
#5
A B Vantamuri, B B Kaliwal
A novel laccase-producing white-rot fungus, Marasmius sp. BBKAV79 (Genbank Accession Number-KP455496, KP455497), was isolated and subjected to purification, characterization and dye decolorization study. The purified enzyme was obtained with a specific activity of 0.226 U mg(-1) protein and a final yield of 13.5 %. The enzyme was found to be a monomeric protein with a molecular mass of ~75 kDa as estimated by non-denaturing polyacrylamide gel electrophoresis (PAGE) and further confirmed with zymogram analysis...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330252/organic-solvent-tolerant-metallo-protease-of-novel-isolate-serratia-marcescens-ppb-26-production-and-characterization
#6
Shikha Thakur, Nirmal Kant Sharma, Neerja Thakur, Savitri, Tek Chand Bhalla
Proteases are a class of enzymes that catalyze hydrolysis of peptide bonds of proteins. In this study, 221 proteolytic bacterial isolates were obtained by enrichment culture method from soils of various regions of Himachal Pradesh, India. From these a hyper producer of protease was screened and identified by morphological and physiological testing and by 16S rDNA sequence as Serratia marcescens PPB-26. Statistical optimization of physiochemical parameters enhanced the protease production by 75 %. Protease of S...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330251/stenotrophomonas-sp-rzs-7-a-novel-phb-degrader-isolated-from-plastic-contaminated-soil-in-shahada-maharashtra-western-india
#7
S J Wani, S S Shaikh, B Tabassum, R Thakur, A Gulati, R Z Sayyed
This paper reports an isolation and identification of novel poly-β-hydroxybutyrate (PHB) degrading bacterium Stenotrophomonas sp. RZS 7 and studies on its extracellular PHB degrading depolymerase enzyme. The bacterium isolated from soil samples of plastic contaminated sites of municipal area in Shahada, Maharashtra, Western India. It was identified as Stenotrophomonas sp. RZS 7 based on polyphasic approach. The bacterium grew well in minimal salt medium (MSM) and produced a zone (4.2 mm) of PHB hydrolysis on MSM containing PHB as the only source of nutrient...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330244/elucidation-of-impact-of-heavy-metal-pollution-on-soil-bacterial-growth-and-extracellular-polymeric-substances-flexibility
#8
Muniswamy David, Paidi Murali Krishna, Jeybalan Sangeetha
Metal bioaccessibility is an alarming issue in croplands of mining sites due to overloading of toxic metals. Hence, the present study is aimed to determine the overloading of toxic metal in croplands across the Tawag village, Hutti, Raichur, India. Correspondingly, to identify the soil bacterial growth, physiological oxidative stress enzyme activity and surface macromolecular functional group evolution were analysed in and around the toxic metal contaminated sites through FT-IR and FT-Raman spectrometry. The evaluated results attribute that the study area is heavily polluted with the toxic metals such as arsenic, cadmium, chromium, lead and zinc...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330240/a-novel-cyclodextrin-glucanotransferase-from-an-alkaliphile-microbacterium-terrae-knr-9-purification-and-properties
#9
Kiransinh N Rajput, Kamlesh C Patel, Ujjval B Trivedi
Cyclodextrin glucanotransferase (CGTase, EC. 2.1.1.19) produced using new alkaliphile Microbacterium terrae KNR 9 has been purified to homogeneity in a single step by the starch adsorption method. The specific activity of the purified CGTase was 45 U/mg compared to crude 0.9 U/mg. This resulted in a 50-fold purification of the enzyme with 33 % yield. The molecular weight of the purified enzyme was found to be 27.72 kDa as determined by SDS-PAGE. Non-denaturing gel electrophoresis and activity staining confirmed the presence of CGTase in crude and the ammonium sulfate precipitate fraction...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330201/dual-action-of-chromium-reducing-and-nitrogen-fixing-bacillus-megaterium-asnf3-for-improved-agro-rehabilitation-of-chromium-stressed-soils
#10
Sumaira Aslam, Ali Hussain, Javed Iqbal Qazi
We conducted a study for enhanced biological rehabilitation of chromium-contaminated soils using a chromium-reducing and nitrogen-fixing bacterial species (Bacillus megaterium-ASNF3). The bacterial species was isolated from a chromium-rich land area, characterized, and employed under optimum conditions for the treatment of artificially prepared chromium-rich soil. The bacterium reduced Cr(VI) up to 86 % in a 60-day trial of incubation in the soil bioreactor. The nitrogenase activity of the bacterium yielded up to 486 nmol of ethylene/mL/h after an incubation period of 40 days when it was optimally cultured in growth medium at neutral pH and 30 °C...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330194/exploited-application-of-sulfate-reducing-bacteria-for-concomitant-treatment-of-metallic-and-non-metallic-wastes-a-mini-review
#11
REVIEW
Ali Hussain, Ali Hasan, Arshad Javid, Javed Iqbal Qazi
A variety of multidimensional anthropogenic activities, especially of industrial level, are contaminating our aquatic and terrestrial environments with a variety of metallic and non-metallic pollutants. The metallic and non-metallic pollutants addressed specifically in this review are heavy metals and various compound forms of sulfates, respectively. Direct and indirect deleterious effects of the both types of pollutants to all forms of life are well-known. The treatment of such pollutants is therefore much necessary before their final discharge into the environment...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330188/purification-and-characterization-of-a-surfactant-compatible-lipase-from-aspergillus-tamarii-jgif06-exhibiting-energy-efficient-removal-of-oil-stains-from-polycotton-fabric
#12
Arijit Das, Srividya Shivakumar, Sourav Bhattacharya, Sujina Shakya, S S Swathi
An extracellular lipase with 23,666.66 U/ml/min activity was produced by Aspergillus tamarii JGIF06 under submerged fermentation in mineral salt medium containing coconut oil (2.5 % v/v), tryptone (2 % w/v) and ammonium chloride (2 % w/v), with initial pH of 5 ± 0.2, incubated at 25 °C for 7 days on a rotary shaker at 120 rpm. A 7.9-fold increase in lipase-specific activity was recorded after purification by DEAE Sepharose ion exchange and Sephadex G200 column chromatography. The apparent molecular mass of this enzyme was revealed as 50 kDa by sodium dodecyl sulphate polyacrylamide gel electrophoresis...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330171/purification-and-characterization-of-endo-%C3%AE-1-4-d-glucanase-from-trichoderma-harzianum-strain-hzn11-and-its-application-in-production-of-bioethanol-from-sweet-sorghum-bagasse
#13
Zabin K Bagewadi, Sikandar I Mulla, Harichandra Z Ninnekar
An acidophilic-solvent-thermostable endo β-1,4-D-glucanase produced from a potential Trichoderma harzianum strain HZN11 was purified to homogeneity by DEAE-Sepharose and Sephadex G-100 chromatography with 33.12 fold purification with specific activity of 66.25 U/mg and molecular mass of ~55 kDa. The optimum temperature and pH were 60 °C and 5.5 retaining 76 and 85 % of activity after 3 h, respectively. It showed stability between pH 4.5-6.0 and temperature between 50-70 °C indicating thermostability...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330168/metabolism-of-waste-engine-oil-by-pseudomonas-species
#14
Lateef B Salam
Two bacterial strains phylogenetically identified as Pseudomonas aeruginosa strains RM1 and SK1 displayed extensive degradation ability on waste engine oil (SAE 40W) in batch cultures. Spectrophotometric analysis revealed the presence of various heavy metals such as lead, chromium and nickel in the waste engine oil. The rate of degradation of waste engine oil by the isolates, for the first 12 days and the last 9 days were 66.3, 31.6 mg l(-1) day(-1)  and 69.6, 40.0 mg l(-1) day(-1) for strains RM1 and SK1, respectively...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330153/an-evaluation-of-the-major-factors-influencing-the-removal-of-copper-ions-using-the-egg-shell-dromaius-novaehollandiae-chitosan-agaricus-bisporus-composite
#15
R K Anantha, S Kota
Rapid industrialisation, technological development, urbanization and increase in population in the recent past coupled with unplanned and unscientific disposal methods led to increased heavy metal levels in water. Realizing the need for development of eco-friendly and cost effective methods, the present investigation was done for the adsorptive removal of copper from aqueous solutions with Dromaius novaehollandiae eggshell and chitosan composite. By one variable at a time method, the optimum contact time was found to be 60 min with an adsorbent dosage of 8 g/L at pH 6, initial adsorbate concentration of 20 mg/L and temperature 30 °C...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330134/phytases-from-enterobacter-and-serratia-species-with-desirable-characteristics-for-food-and-feed-applications
#16
Harpreet Kaur Kalsi, Rajveer Singh, Harcharan Singh Dhaliwal, Vinod Kumar
Phytases are enzymes of great industrial importance with wide range of applications in animal and human nutrition. These catalyze the hydrolysis of phosphomonoester bonds in phytate, thereby releasing lower forms of myo-inositol phosphates and inorganic phosphate. Addition of phytase to plant-based foods can improve its nutritional value and increase mineral bioavailability by decreasing nutritional effect of phytate. In the present investigation, 43 phytase positive bacteria on PSM plates were isolated from different sources and characterized for phytase activity...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330122/production-purification-and-characterization-of-a-thermotolerant-alkaline-serine-protease-from-a-novel-species-bacillus-caseinilyticus
#17
Thirumala Mothe, Vishnuvardhan Reddy Sultanpuram
Alkaline proteases are important enzymes in many industrial applications, especially as additives in laundry detergent industry. Though there are a number of Bacillus species which are reported to be producing proteases, the efficiency of a protease produced by a novel strain has to be studied in comparison to the others. Hence, in this study, an alkaline serine protease produced by a novel species Bacillus caseinilyticus was purified and characterized for its possible usage in detergent industry. Ammonium sulphate, dialysis and DEAE column chromatographic methods were used for purification of the isolated alkaline protease...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330088/metals-induced-functional-stress-in-sulphate-reducing-thermophiles
#18
Ali Hussain, Javed Iqbal Qazi
All toxic metals have been known to inhibit different activities of sulphate-reducing bacteria (SRB) at different concentrations. The present study delineates functional responses of two thermophilic SRB species (Desulfotomaculum reducens-HA1 and Desulfotomaculum hydrothermale-HA2) to toxic metals. Bacterial activity was assessed in terms of sulphate reduction and metal precipitation employing four concentrations (1, 5, 10 and 15 ppm) of three dissolved toxic metals (Cu, Cr and Ni) independently. Both sulphidogenic bacterial species showed results in a very narrow range of fluctuations...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28329756/functional-materials-discovery-using-energy-structure-function-maps
#19
Angeles Pulido, Linjiang Chen, Tomasz Kaczorowski, Daniel Holden, Marc A Little, Samantha Y Chong, Benjamin J Slater, David P McMahon, Baltasar Bonillo, Chloe J Stackhouse, Andrew Stephenson, Christopher M Kane, Rob Clowes, Tom Hasell, Andrew I Cooper, Graeme M Day
Molecular crystals cannot be designed in the same manner as macroscopic objects, because they do not assemble according to simple, intuitive rules. Their structures result from the balance of many weak interactions, rather than from the strong and predictable bonding patterns found in metal-organic frameworks and covalent organic frameworks. Hence, design strategies that assume a topology or other structural blueprint will often fail. Here we combine computational crystal structure prediction and property prediction to build energy-structure-function maps that describe the possible structures and properties that are available to a candidate molecule...
March 22, 2017: Nature
https://www.readbyqxmd.com/read/28329638/developing-a-continuous-quality-improvement-assessment-using-a-patient-centered-approach-in-optimizing-sle-disease-control
#20
Katelyn Updyke, Brittany Urso, James A Solomon
Systemic lupus erythematous is a multi-organ autoimmune disease in which the patient has "lost" self- tolerance and developed immune-complexes containing self-antigen that cause disease through tissue inflammation and multi-organ damage. Patients often have unpredictable flares in symptoms with poorly identified triggers. However, it has been suggested in other literature that exogenous exposures, including ingested beef, cigarette smoke, UV radiation, metal-complexing agents, and infections can contribute to exacerbations and improvements in SLE symptoms...
September 15, 2016: Dermatology Online Journal
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