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Ingenuity pathway analysis

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https://www.readbyqxmd.com/read/28549041/value-of-osteoblast-derived-exosomes-in-bone-diseases
#1
Min Ge, Yingzhi Wu, Ronghu Ke, Tianyi Cai, Junyi Yang, Xiongzheng Mu
PURPOSE: The authors' purpose is to reveal the value of osteoblast-derived exosomes in bone diseases. METHODS: Microvesicles from supernatants of mouse Mc3t3 were isolated by ultracentrifugation and then the authors presented the protein profile by proteomics analysis. RESULTS: The authors detected a total number of 1536 proteins by mass spectrometry and found 172 proteins overlap with bone database. The Ingenuity Pathway Analysis shows network of "Skeletal and Muscular System Development and Function, Developmental Disorder, Hereditary Disorder" and pathway about osteogenesis...
May 25, 2017: Journal of Craniofacial Surgery
https://www.readbyqxmd.com/read/28539318/serum-estradiol-levels-in-controlled-ovarian-stimulation-directly-affect-the-endometrium
#2
Kamran Ullah, Tanzil Ur Rahman, Hai-Tao Pan, Meng-Xi Guo, Xin-Yan Dong, Juan Liu, Lu-Yang Jin, Yi Cheng, Zhang-Hong Ke, Jun Ren, Xian-Hua Lin, Xiao-Xiao Qiu, Ting-Ting Wang, He-Feng Huang, Jian-Zhong Sheng
Previous studies have shown that increasing concentrations of estradiol had a toxic effect on the embryo and were deleterious to embryo adhesion. In this study, we evaluated the physiological impact of estradiol levels on endometrial cells to unveil that serum estradiol levels probably targeted endometrium in controlled ovarian hyperstimulation (COH) protocols. An attachment model of human choriocarcinoma (JAr) cell spheroids to receptive-phase endometrial epithelial cells and Ishikawa cells treated with different estradiol (10-9M or 10-7M) levels was developed...
May 24, 2017: Journal of Molecular Endocrinology
https://www.readbyqxmd.com/read/28536832/proteomic-profiling-reveals-crucial-retinal-protein-alterations-in-the-early-phase-of-an-experimental-glaucoma-model
#3
Fabian Anders, Julia Teister, Sebstian Funke, Norbert Pfeiffer, Franz Grus, Thanos Solon, Verena Prokosch
PURPOSE: Clinical glaucoma is difficult to assess in terms of molecular pathophysiology, prompting studies in experimental models of glaucoma. The purpose of this study was to investigate quantitative changes in retinal protein expression at the onset of experimental glaucoma in rats. Analyzing the proteome provides a suitable tool to decipher the pathophysiological processes in glaucomatous degeneration. METHODS: Thermic cauterization of episcleral veins was utilized to elevate the intraocular pressure in Sprague Dawley rats...
May 24, 2017: Graefe's Archive for Clinical and Experimental Ophthalmology
https://www.readbyqxmd.com/read/28535982/altered-gene-expression-in-early-postnatal-monoamine-oxidase-a-knockout-mice
#4
Kevin Chen, Abbey Kardys, Yibu Chen, Stephen Flink, Boris Tabakoff, Jean C Shih
We reported previously that monoamine oxidase (MAO) A knockout (KO) mice show increased serotonin (5-hydroxytryptamine, 5-HT) levels and autistic-like behaviors characterized by repetitive behaviors, and anti-social behaviors. We showed that administration of the serotonin synthesis inhibitor para-chlorophenylalanine (pCPA) from post-natal day 1 (P1) through 7 (P7) in MAO A KO mice reduced the serotonin level to normal and reverses the repetitive behavior. These results suggested that the altered gene expression at P1 and P7 may be important for the autistic-like behaviors seen in MAO A KO mice and was studied here...
May 20, 2017: Brain Research
https://www.readbyqxmd.com/read/28524240/potential-serum-biomarkers-associated-with-mild-and-severe-leptospirosis-infection-a-cohort-study-in-the-malaysian-population
#5
Tan Xue Ting, Fairuz Binti Amran, Ravindran Thayan, Norazah Ahmad, Roslinda Jaafar, Rahimah Haron, Rafidah Abdullah, Sazwan Reezal Bin Shamsuddin, Nor Suhaila Binti Md Riffin, Puteri Shafinaz Abdul-Rahman
Leptospirosis is an emerging zoonotic infectious disease in Malaysia. The symptoms of leptospirosis vary from mild non-specific flu-like illness to a severe condition which is usually associated with serious complication and fatality. To study the protein expression profile of mild and severe leptospirosis, fifteen paired sera were collected from the patients who were mildly infected and following that progressed to severe stage. The proteome profiles of mild and severe cases were studied using 2DE analysis in combination with LC-MS/MS...
May 19, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28523741/comparative-membrane-proteomics-analyses-of-breast-cancer-cell-lines-to-understand-the-molecular-mechanism-of-breast-cancer-brain-metastasis
#6
Wenjing Peng, Yu Zhang, Rui Zhu, Yehia Mechref
Breast cancer is the leading type of cancer in women. Breast cancer brain metastasis is currently considered an issue of concern among breast cancer patients. Membrane proteins play important roles in breast cancer brain metastasis, involving cell adhesion and penetration of blood-brain barrier. To understand the mechanism of breast cancer brain metastasis, liquid chromatography-tandem mass spectrometry (LC-MS/MS) was employed in conjunction with enrichment of membrane proteins to analyze the proteomes from 5 different breast cancer and a brain cancer cell lines...
May 19, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28523289/core-canonical-pathways-involved-in-developing-human-glioblastoma-multiforme-gbm
#7
Somiranjan Ghosh, Sisir Dutta, Gabriel Thorne, Ava Boston, Alexis Barfield, Narendra Banerjee, Rayshawn Walker, Hirendra Nath Banerjee
Glioblastoma multiforme (GBM) is the most common and aggressive type of the primary brain tumors with pathologic hallmarks of necrosis and vascular proliferation. The diagnosis of GBM is currently mostly based on histological examination of brain tumor tissues, after radiological characterization and surgical biopsy. The ability to characterize tumors comprehensively at the molecular level raises the possibility that diagnosis can be made based on molecular profiling with or without histological examination, rather than solely on histological phenotype...
February 2017: Int J Sci Res Sci Eng Technol
https://www.readbyqxmd.com/read/28522250/ppargamma-agonists-rescue-increased-phosphorylation-of-fgf14-at-s226-in-the-tg2576-mouse-model-of-alzheimer-s-disease
#8
Wei-Chun J Hsu, Norelle C Wildburger, Sigmund J Haidacher, Miroslav N Nenov, Oluwarotimi Folorunso, Aditya K Singh, Brent C Chesson, Whitney F Franklin, Ibdanelo Cortez, Rovshan G Sadygov, Kelly T Dineley, Jay S Rudra, Giulio Taglialatela, Cheryl F Lichti, Larry Denner, Fernanda Laezza
BACKGROUND: Cognitive impairment in humans with Alzheimer's disease (AD) and in animal models of Aβ-pathology can be ameliorated by treatments with the nuclear receptor peroxisome proliferator-activated receptor-gamma (PPARγ) agonists, such as rosiglitazone (RSG). Previously, we demonstrated that in the Tg2576 animal model of AD, RSG treatment rescued cognitive deficits and reduced aberrant activity of granule neurons in the dentate gyrus (DG), an area critical for memory formation...
May 15, 2017: Experimental Neurology
https://www.readbyqxmd.com/read/28521489/identification-of-proteins-with-different-abundance-associated-with-cell-migration-and-proliferation-in-leiomyoma-interstitial-fluid-by-proteomics
#9
Blendi Ura, Federica Scrimin, Cinzia Franchin, Giorgio Arrigoni, Danilo Licastro, Lorenzo Monasta, Giuseppe Ricci
Uterine leiomyoma is the most common female reproductive tract benign tumor. Little is known about protein composition and changes in the leiomyoma interstitial fluid (IF). The present study focused on changes in protein abundance in the IF of leiomyoma. Leiomyoma IFs and adjacent myometrial IFs were obtained and analyzed by two-dimensional electrophoresis (2-DE) coupled with mass spectrometry and western blotting for 2-DE data validation. A total of 25 unique proteins were observed to change significantly (P<0...
May 2017: Oncology Letters
https://www.readbyqxmd.com/read/28515692/wu-tou-decoction-in-rheumatoid-arthritis-integrating-network-pharmacology-and-in-vivo-pharmacological-evaluation
#10
Qingqing Guo, Kang Zheng, Danping Fan, Yukun Zhao, Li Li, Yanqin Bian, Xuemei Qiu, Xue Liu, Ge Zhang, Chaoying Ma, Xiaojuan He, Aiping Lu
Purpose: This study aimed to explore underlying action mechanism of Wu-Tou decoction (WTD) in rheumatoid arthritis (RA) through network pharmacology prediction and experimental verification. Methods: Chemical compounds and human target proteins of WTD as well as RA-related human genes were obtained from TCM Database @ Taiwan, PubChem and GenBank, respectively. Subsequently, molecular networks and canonical pathways presumably involved in the treatment of WTD on RA were generated by ingenuity pathway analysis (IPA) software...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28504421/bioinformatics-analysis-on-molecular-mechanism-of-green-tea-compound-epigallocatechin-3-gallate-against-ovarian-cancer
#11
S Xinqiang, Z Mu, C Lei, L Y Mun
Epigallocatechin-3-gallate (EGCG) is the most abundant and biologically active catechin in green tea, and it exerts multiple effects in humans through mechanisms that remain to be clarified. The present study used bioinformatics to identify possible mechanisms by which EGCG reduces the risk of ovarian cancer. Possible human protein targets of EGCG were identified in the PubChem database, possible human gene targets were identified in the National Center for Biotechnology Information database, and then both sets of targets were analyzed using Ingenuity Pathway Analysis (IPA)...
May 15, 2017: Clinical and Translational Science
https://www.readbyqxmd.com/read/28503943/transcriptional-profile-of-ethylene-glycol-monomethyl-ether-induced-testicular-toxicity-in-rats
#12
Takuya Matsuyama, Koichi Yabe, Chiharu Kuwata, Kazumi Ito, Yosuke Ando, Hiroshi Iida, Kazuhiko Mori
To clarify the molecular mechanism of ethylene glycol monomethyl ether (EGME)-induced testicular toxicity, the potential for EGME-related changes in transcript levels of genes including spermatocyte-specific genes was evaluated in the testis of rats given single dosing of EGME at 200, 600, or 2000 mg/kg. Furthermore, the contribution of decreased testicular testosterone on EGME-induced spermatocyte toxicity was investigated by comparing to transcriptional profile due to a testosterone synthesis inhibitor, ketoconazole (KET), at 30 or 300 mg/kg...
May 15, 2017: Drug and Chemical Toxicology
https://www.readbyqxmd.com/read/28499042/proteomic-analysis-reveals-changes-in-carbohydrate-and-protein-metabolism-associated-with-broiler-breast-myopathy
#13
Vivek A Kuttappan, Walter Bottje, Ranjith Ramnathan, Steven D Hartson, Craig N Coon, Byung-Whi Kong, Casey M Owens, Mercedes Vazquez-Añon, Billy M Hargis
White Striping (WS) and Woody Breast (WB) are 2 conditions that adversely affect consumer acceptance as well as quality of poultry meat and meat products. Both WS and WB are characterized with degenerative myopathic changes. Previous studies showed that WS and WB in broiler fillets could result in higher ultimate pH, increased drip loss, and decreased marinade uptake. The main objective of the present study was to compare the proteomic profiles of muscle tissue (n = 5 per group) with either NORM (no or few minor myopathic lesions) or SEV (with severe myopathic changes)...
May 12, 2017: Poultry Science
https://www.readbyqxmd.com/read/28498421/molecular-analysis-of-the-mouse-brain-exposed-to-chronic-mild-stress-the-influence-of-hepatocyte-nuclear-factor-4%C3%AE-on-physiological-homeostasis
#14
Kaoru Ikubo, Kyosuke Yamanishi, Nobutaka Doe, Takuya Hashimoto, Miho Sumida, Yuko Watanabe, Yosif El-Darawish, Wen Li, Haruki Okamura, Hiromichi Yamanishi, Hisato Matsunaga
Major depressive disorder (MDD) is a prevalent disorder that causes considerable disability in social functioning and is a risk factor for physical diseases. Recent clinical reports have demonstrated a marked association between MDD and physiological dyshomeostasis induced by metabolic disorders, including diabetes, hormone abnormalities and autoimmune diseases. The authors of the present study have previously analyzed comparative gene expression profiles in the prefrontal cortex (PFC) of a chronic mild stress (CMS) animal model of MDD...
May 11, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28490276/endometrial-stromal-and-epithelial-cells-exhibit-unique-aberrant-molecular-defects-in-patients-with-endometriosis
#15
Philip C Logan, Pamela Yango, Nam D Tran
CONTEXT: Endometriosis is a chronic inflammatory disease that causes pain and infertility in women of reproductive age. OBJECTIVE: To investigate the pathologic pathways in endometrial stromal and epithelial cells that contribute to the manifestation of endometriosis. DESIGN: In vitro cellular and molecular analyses of isolated eutopic endometrial stromal and epithelial cells. METHODS: Eutopic stromal and epithelial cells from endometriotic and normal patients were isolated by fluorescence-activated cell sorting for paired sibling RNA sequencing and microRNA microarray...
January 1, 2017: Reproductive Sciences
https://www.readbyqxmd.com/read/28486780/quantitative-proteomics-to-study-a-small-molecule-targeting-the-loss-of-von-hippel-lindau-in-renal-cell-carcinomas
#16
Nadia Bouhamdani, Andrew Joy, David Barnett, Kevin Cormier, Daniel Léger, Ian C Chute, Simon Lamarre, Rodney Ouellette, Sandra Turcotte
Inactivation of the tumor suppressor gene, von Hippel-Lindau (VHL), is known to play an important role in the development of sporadic clear cell renal cell carcinomas (ccRCCs). Even if available targeted therapies for metastatic RCCs (mRCCs) have helped to improve progression-free survival rates, they have no durable clinical response. We have previously shown the feasibility of specifically targeting the loss of VHL with the identification of a small molecule, STF-62247. Understanding its functionality is crucial for developing durable personalized therapeutic agents differing from those available targeting hypoxia inducible factor (HIF-) pathways...
May 9, 2017: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/28484274/monocytic-microrna-profile-associated-with-coronary-collateral-artery-function-in-chronic-total-occlusion-patients
#17
Nazanin Hakimzadeh, Joëlle Elias, Gilbert W M Wijntjens, Ruud Theunissen, Angela van Weert, Martijn W Smulders, Nynke van den Akker, Perry D Moerland, Hein J Verberne, Loes P Hoebers, Jose P S Henriques, Anja M van der Laan, Mustafa Ilhan, Mark Post, Sebastiaan C A M Bekkers, Jan J Piek
An expansive collateral artery network is correlated with improved survival in case of adverse cardiac episodes. We aimed to identify cellular microRNAs (miRNA; miR) important for collateral artery growth. Chronic total occlusion (CTO) patients (n = 26) were dichotomized using pressure-derived collateral flow index (CFIp) measurements; high collateral capacity (CFIp > 0.39; n = 14) and low collateral (CFIp < 0.39; n = 12) capacity. MiRNA profiling via next generation sequencing from various monocyte phenotypes (freshly isolated monocytes, monocytes cultured without stimulant, or stimulation with lipopolysaccharide, interleukin 4, transforming growth factor beta-1, or interferon gamma) revealed significantly different miRNA expression patterns between high versus low collateral capacity patients...
May 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28479159/proteomic-analysis-of-filaggrin-deficiency-identifies-molecular-signatures-characteristic-of-atopic-eczema
#18
Martina S Elias, Heather A Long, Carla F Newman, Paul A Wilson, Andrew West, Paul J McGill, Keith C Wu, Michael J Donaldson, Nick J Reynolds
BACKGROUND: Atopic eczema (AE) is characterized by skin barrier and immune dysfunction. Null mutations in filaggrin (FLG), a key epidermal barrier protein, strongly predispose to AE; however, the precise role of FLG deficiency in AE pathogenesis remains incompletely understood. OBJECTIVES: We sought to identify global proteomic changes downstream of FLG deficiency in human epidermal living skin-equivalent (LSE) models and validate findings in skin of patients with AE...
April 28, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28476498/molecular-targets-of-chloropicrin-in-human-airway-epithelial-cells
#19
Maija Pesonen, Jaana Rysä, Markus Storvik, Kirsi Rilla, Markku Pasanen, Kirsi Vähäkangas
Chloropicrin is a vaporizing, irritating compound that causes complications in the respiratory system when inhaled. In this study, we examined the effects of exposure to chloropicrin for 24h on ultrastructure and global gene expression in primary human bronchial epithelial cells. The treatment increased the number of round and shrunken cells, which detached from culture plates more readily than the untreated control cells. Transmission electron microscopy revealed some swollen mitochondria and the appearance of autophagy/lysosome type of vacuoles in the treated cells...
May 3, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28476192/gene-expression-profiling-of-acute-lymphoblastic-leukemia-in-children-with%C3%A2-very-early-relapse
#20
Juan Carlos Núñez-Enríquez, Diego Alberto Bárcenas-López, Alfredo Hidalgo-Miranda, Elva Jiménez-Hernández, Vilma Carolina Bekker-Méndez, Janet Flores-Lujano, Karina Anastacia Solis-Labastida, Gabriela Bibiana Martínez-Morales, Fausto Sánchez-Muñoz, Laura Eugenia Espinoza-Hernández, Martha Margarita Velázquez-Aviña, Laura Elizabeth Merino-Pasaye, Alejandra Jimena García Velázquez, María Luisa Pérez-Saldívar, Raúl Mojica-Espinoza, Julián Ramírez-Bello, Silvia Jiménez-Morales, Juan Manuel Mejía-Aranguré
BACKGROUND AND AIMS: Acute lymphoblastic leukemia (ALL) is the most common childhood cancer worldwide. Mexican patients have high mortality rates, low frequency of good prognosis biomarkers (i.e., ETV6-RUNX1) and a high proportion is classified at the time of diagnosis with a high risk to relapse according to clinical features. In addition, very early relapses are more frequently observed than in other populations. The aim of the study was to identify new potential biomarkers associated with very early relapse in Mexican ALL children through transcriptome analysis...
November 2016: Archives of Medical Research
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