keyword
MENU ▼
Read by QxMD icon Read
search

Hbx

keyword
https://www.readbyqxmd.com/read/29352124/hbv-infection-potentiates-resistance-to-s-phase-arrest-inducing-chemotherapeutics-by-inhibiting-chk2-pathway-in-diffuse-large-b-cell-lymphoma
#1
Xinying Zhao, Xudong Guo, Libo Xing, Wenqin Yue, Haisen Yin, Miaoxia He, Jianmin Wang, Jianmin Yang, Jie Chen
A considerable number of diffuse large B-cell lymphoma (DLBCL) patients are infected with hepatitis B virus (HBV), which is correlated with their poor outcomes. However, the role of HBV infection in DLBCL treatment failure remains poorly understood. Here, our data demonstrated that HBV infection was closely associated with poorer clinical prognosis independent of its hepatic dysfunction in germinal center B-cell type (GCB type) DLBCL patients. Interestingly, we found that DLBCL cells expressing hepatitis B virus X protein (HBX) did not exhibit enhanced cell growth but did show reduced sensitivity to methotrexate (MTX) and cytarabine (Ara-C), which induced S-phase arrest...
January 19, 2018: Cell Death & Disease
https://www.readbyqxmd.com/read/29350888/identification-of-p90-ribosomal-s6-kinase-2-as-a-novel-host-protein-in-hbx-augmenting-hbv-replication-by-itraq-based-quantitative-comparative-proteomics
#2
Li-Bo Yan, You-Jia Yu, Qing-Bo Zhang, Xiao-Qiong Tang, Lang Bai, FeiJun Huang, Hong Tang
PURPOSE: The aim of this study was to screen for novel host proteins that play a role in HBx augmenting HBV replication. EXPERIMENTAL DESIGN: Three HepG2 cell lines stably harboring different functional domain of HBx (HBx, HBx-Cm6, and HBx-Cm16) were cultured. ITRAQ technology integrated with LC-MS/MS analysis was applied to identify the proteome differences among these three cell lines. RESULTS: In brief, a total of 70 different proteins were identified among HepG2-HBx, HepG2-HBx-Cm6, and HepG2-HBx-Cm16 by double repetition...
January 19, 2018: Proteomics. Clinical Applications
https://www.readbyqxmd.com/read/29328380/role-of-the-pi3k%C3%A2-akt%C3%A2-mtor-pathway-in-hepatitis-b-virus-infection-and-replication
#3
Kunlun Xiang, Bin Wang
The replication of hepatitis B virus (HBV) may be modulated by a variety of cell signaling pathways, including the phosphatidylinositol 3‑kinase (PI3K)‑RAC‑α serine/threonine‑protein kinase (AKT)‑serine/threonine‑protein kinase mTOR (mTOR) pathway. The aim of the present study was to determine the regulatory effects of this pathway on the infection and replication of HBV. The results indicated that the HBV entry process may activate the AKT pathway, as demonstrated by AKT phosphorylation in HBV natural infection...
January 8, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29316268/crosstalk-between-hepatitis-b-virus-x-and-high-mobility-group-box-1-facilitates-autophagy-in-hepatocytes
#4
Sha Fu, Juan Wang, Xingwang Hu, Rong-Rong Zhou, Yongming Fu, Daolin Tang, Rui Kang, Yan Huang, Lunquan Sun, Ning Li, Xue-Gong Fan
Hepatitis B virus (HBV) X (HBx) protein is a pivotal regulator of HBV-triggered autophagy. However, the role of HBx-induced epigenetic changes in autophagy remains largely unknown. The cytoplasmic high-mobility group box 1 (HMGB1) has been identified as a positive regulator of autophagy, and its cytoplasmic translocation is closely associated with its acetylation status. Here, we evaluated the function of HMGB1 in HBx-mediated autophagy and its association with histone deacetylase (HDAC). Using cell lines with enforced expression of HBx, we demonstrated that HBx upregulated the expression of HMGB1 and promoted its cytoplasmic translocation by acetylation to facilitate autophagy...
January 5, 2018: Molecular Oncology
https://www.readbyqxmd.com/read/29307794/hepatitis-b-virus-x-protein-modulates-cytosolic-ca2-signaling-in-primary-human-hepatocytes
#5
Jessica C Casciano, Michael J Bouchard
Worldwide, approximately 240 million people are chronically infected with the hepatitis B virus (HBV); chronic HBV infection is associated with the development of life-threatening liver diseases. The HBV HBx protein alters hepatocyte physiology to promote HBV replication. We previously reported that HBx modulates calcium signaling to stimulate HBV replication in human hepatoblastoma HepG2 cells and primary rat hepatocytes. Whether HBx modulates calcium signaling in a primary human hepatocyte, the natural site of an HBV infection, has not been determined...
January 4, 2018: Virus Research
https://www.readbyqxmd.com/read/29290826/a-cell-penetrating-whole-molecule-antibody-targeting-intracellular-hbx-suppresses-hepatitis-b-virus-via-trim21-dependent-pathway
#6
Jun-Fang Zhang, Hua-Long Xiong, Jia-Li Cao, Shao-Juan Wang, Xue-Ran Guo, Bi-Yun Lin, Ying Zhang, Jing-Hua Zhao, Ying-Bin Wang, Tian-Ying Zhang, Quan Yuan, Jun Zhang, Ning-Shao Xia
Rationale: Monoclonal antibodies (mAbs) mostly targeting extracellular or cell surface molecules have been widely used in the treatment of various diseases. However, mAbs cannot pass through the cell membrane as efficiently as small compounds, thus limiting their use against intracellular targets. Methods to shuttle antibodies into living cells may largely expand research and application in areas based on mAbs. Hepatitis B virus X protein (HBx) is an important intracellular multi-functional viral protein in the life cycle of hepatitis B virus (HBV)...
2018: Theranostics
https://www.readbyqxmd.com/read/29285238/hepatitis-b-virus-hbv-x-gene-mutations-and-their-association-with-liver-disease-progression-in-hbv-infected-patients
#7
Ahmed A Al-Qahtani, Mashael R Al-Anazi, Nyla Nazir, Rohit Ghai, Ayman A Abdo, Faisal M Sanai, Waleed K Al-Hamoudi, Khalid A Alswat, Hamad I Al-Ashgar, Mohammed Q Khan, Ali Albenmousa, Damian Dela Cruz, Marie Fe F Bohol, Mohammed N Al-Ahdal
Hepatitis B virus (HBV) is one of the most widespread human pathogens causing chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma (HCC). This study investigated the clinical impact of single and combinational mutations in HBx gene on the pathogenesis of HCC during progressive stages of liver disease. The patients were categorized into inactive HBV carriers, active carriers, cirrhosis and HCC groups based on disease severity. Male sex, age > 50 years, and high serum alanine aminotransferase level were associated with risk of progressive liver disease...
December 1, 2017: Oncotarget
https://www.readbyqxmd.com/read/29285141/interferon-%C3%AE-inhibits-cell-migration-and-invasion-and-induces-the-expression-of-antiviral-proteins-in-huh-7-cells-transfected-with-hepatitis-b-virus-x-gene-expressing-lentivirus
#8
Qian Yang, Xiao-Peng Li, Yuan-Bin Zhong, Tian-Xin Xiang, Lun-Li Zhang
Hepatitis B virus (HBV) X protein (HBx) serves an important role in HBV infection and the development of HBV-related liver cancer. Interferon-α (IFN-α) is used to treat patients with HBV; however, the role of IFN-α in the development of HBV-related liver cancer remains unclear. The present study established a new HBV-related liver cancer model (Huh-7-HBx) by transfecting the hepatoma cell line Huh-7, with HBx-expressing lentivirus. Following IFN-α treatment, cell viability, migration and invasion, as well as the expression of antiviral proteins in Huh-7-HBx, were subsequently determined...
December 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29258558/hepatitis-b-virus-x-protein-induces-epithelial-mesenchymal-transition-of-hepatocellular-carcinoma-cells-by-regulating-long-non-coding-rna
#9
Yinji Jin, Di Wu, Weiwei Yang, Mingjiao Weng, Yafei Li, Xuefei Wang, Xiao Zhang, Xiaoming Jin, Tianzhen Wang
BACKGROUND: It has been widely accepted that hepatitis B virus X protein (HBx) plays an important role in hepatocellular carcinoma (HCC). This study aimed to explore the function of long non-coding RNAs (lncRNAs) in the epithelial-mesenchymal transition (EMT) induced by HBx. METHODS: The association between HBx and EMT markers was detected using immunohistochemistry in HCC tissues. The effect of HBx on HCC EMT was assessed through morphological analysis, transwell assay, metastatic in vivo study and detection of EMT markers...
December 19, 2017: Virology Journal
https://www.readbyqxmd.com/read/29236306/rs4705342-polymorphism-is-involved-in-the-tumorigenesis-of-hbv-positive-hcc-by-altering-the-binding-affinity-of-hbv-induced-nf-kb-with-the-promoter-region-of-microrna-143
#10
Yin Xiuli, Sun Shiying, Zhao Junyan, Yang Jing, Lei Xiaofei, Xu Changqing, Li Kun
BACKGROUND: The objective of this study was to explore the role of rs4705342 located in the miR-143 promoter in relation to the control of HBV positive HCC and the underlying molecular mechanism. METHOD: A luciferase assay was performed to explore the factors which influenced miR-143 transcription activity and the target gene of miR-143. This would further be confirmed by ChIP assay. Western-blot and real-time PCR were performed to identify the relationship between miR-143 and ORP8...
December 13, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29231260/metformin-inhibits-tumorigenesis-in-hbv-induced-hepatocellular-carcinoma-by-suppressing-hulc-overexpression-caused-by-hbx
#11
Zhen Jiang, Haichao Liu
BACKGROUND: We aimed to understand whether metformin imposes the inhibitory effect on the HBV-associated tumorigenesis by regulating the HULC and its downstream signaling pathway. METHOD: Luciferase assay, RT-PCR and western-blot, MTT and flow cytometry analysis were performed to understand and the mechanism, by which metformin enhance the inhibitory effect on the HBV-associated tumorigenesis by regulating the HULC and its downstream signaling pathway. RESULT: HBX promoted viability of three types of cell lines, while metformin inhibited apoptosis of above two cells...
December 12, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29221184/hepatitis-b-virus-x-protein-inhibits-apoptosis-by-modulating-endoplasmic-reticulum-stress-response
#12
Jia Li, Jiang He, Yongming Fu, Xingwang Hu, Lun-Quan Sun, Yan Huang, Xuegong Fan
Chronic Hepatitis B virus (HBV) infection is a major risk of hepatocellular carcinoma (HCC) worldwide. Hepatitis B virus X protein (HBx) is encoded by one of the four open reading frames of HBV, and is well known as an important coactivator for HBV replication and HBV-associated hepatocellular carcinogenesis. However, its role in keeping cells from apoptosis to promote HCC proliferation remains controversial. Here, we used HBx expressing HCC cells as a model, to investigate the mechanism of HBx-mediated cellular response to endoplasmic reticulum (ER) stress...
November 10, 2017: Oncotarget
https://www.readbyqxmd.com/read/29193612/a-direct-role-for-hepatitis-b-virus-x-protein-in-inducing-mitochondrial-membrane-permeabilization
#13
Hye-Ra Lee, Young Youn Cho, Gi Young Lee, Deok-Gyun You, Young Do Yoo, Yoon Jun Kim
Hepatitis B virus X protein (HBx) acts as a multifunctional protein that regulates intracellular signaling pathways during HBV infection. It has mainly been studied in terms of its interaction with cellular proteins. Here, we show that HBx induces membrane permeabilization independently of the mitochondrial permeability transition pore complex. We generated mitochondrial outer membrane-mimic liposomes to observe the direct effects of HBx on membranes. We found that HBx induced membrane permeabilization, and the region comprising the transmembrane domain and the mitochondrial targeting sequence was sufficient for this process...
November 28, 2017: Journal of Viral Hepatitis
https://www.readbyqxmd.com/read/29183361/naringenin-attenuates-hepatitis-b-virus-x-protein-induced-hepatic-steatosis
#14
Hung-Jen Lin, Ko-Li Ku, I-Hsin Lin, Chia-Chou Yeh
BACKGROUND: Naringenin (Nar), a common dietary flavonoid abundantly present in fruits, vegetables, and Chinese herbs, is believed to possess strong anti-inflammatory properties and to modulate hepatic apolipoprotein and lipid synthesis. However, there are no reports describing Nar's effects on the hepatitis B virus protein X (HBx) -induced hepatic steatosis, and the detailed molecular mechanisms of the compound's effects are still unclear. METHODS: Nar was administered by oral gavage to HBx-transgenic mice from 4 to 6 weeks of age...
November 28, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/29168767/long-non-coding-rnas-in-hepatitis-b-virus-related-hepatocellular-carcinoma-regulation-functions-and-underlying-mechanisms
#15
REVIEW
Lipeng Qiu, Tao Wang, Xiuquan Xu, Yihang Wu, Qi Tang, Keping Chen
Hepatocellular carcinoma (HCC) is the fifth most common cancer and the third leading cause of cancer death in the world. Hepatitis B virus (HBV) and its X gene-encoded protein (HBx) play important roles in the progression of HCC. Although long non-coding RNAs (lncRNAs) cannot encode proteins, growing evidence indicates that they play essential roles in HCC progression, and contribute to cell proliferation, invasion and metastasis, autophagy, and apoptosis by targeting a large number of pivotal protein-coding genes, miRNAs, and signaling pathways...
November 23, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29133292/intracellular-hepatitis-b-virus-increases-hepatic-cholesterol-deposition-in-alcoholic-fatty-liver-via-hepatitis-b-core-protein
#16
Ya-Qi Wang, Ting Wu, Dan-Qing Hu, Xin-Xin Weng, Xiao-Jing Wang, Pei-Jer Chen, Xiao-Ping Luo, Hong-Wu Wang, Qin Ning
Hepatits B virus (HBV) infection is a prevalent infectious disease with serious outcomes like chronic and acute hepatitis, cirrhosis and hepatocellular carcinoma. However, the metabolic alteration by HBV is rarely taken into concerns. With the high prevalence of alcohol consumption and chronic HBV infection, their overlap is assumed as an increasing latent hazard although without exact calculation, moreover, the impact of chronic alcoholic consumption combined with HBV on cholesterol metabolism is unknown. Six-week-old male FVB/Ncrl mice were hydrodynamically injected with a pGEM-4Z-1...
November 13, 2017: Journal of Lipid Research
https://www.readbyqxmd.com/read/29114584/expression-purification-and-characterization-of-hepatitis-b-virus-x-protein-bh3-like-motif-linker-bcl-xl-fusion-protein-for-structural-studies
#17
Hideki Kusunoki, Toshiyuki Tanaka, Toshiyuki Kohno, Hirokazu Kimura, Kazuo Hosoda, Kaori Wakamatsu, Isao Hamaguchi
Hepatitis B virus X protein (HBx) is a multifunctional protein that interacts directly with many host proteins. For example, HBx interacts with anti-apoptotic proteins, Bcl-2 and Bcl-xL, through its BH3-like motif, which leads to elevated cytosolic calcium levels, efficient viral DNA replication and the induction of apoptosis. To facilitate sample preparation and perform detailed structural characterization of the complex between HBx and Bcl-xL, we designed and purified a recombinant HBx BH3-like motif-linker-Bcl-xL fusion protein produced in E...
March 2017: Biochemistry and Biophysics Reports
https://www.readbyqxmd.com/read/29103765/downregulation-of-mir-200a-3p-induced-by-hepatitis-b-virus-x-hbx-protein-promotes-cell-proliferation-and-invasion-in-hbv-infection-associated-hepatocarcinoma
#18
Taiyang Shi, Qinfang Hua, Zhipeng Ma, Qijun Lv
BACKGROUND: Hepatitis B Virus X (HBx) Protein encoded by HBV is believed to be the major player in the process of HBV-induced oncogenesis. Ectopic expression of miR-200a-3p was reported to be associated with diverse tumorigenesis. This study aimed to better understand the role of miR-200a-3p and its correlation with HBx in HBV-induced hepatocellular carcinoma (HCC). METHODS: In this report, we examined the gene expression using quantitative RT-PCR and protein expression using Western blotting analysis...
October 26, 2017: Pathology, Research and Practice
https://www.readbyqxmd.com/read/29089476/tripartite-motif-containing-52-trim52-promotes-cell-proliferation-in-hepatitis-b-virus-associated-hepatocellular-carcinoma
#19
Yi Zhang, Shan-Shan Wu, Xiao-Hua Chen, Zheng-Hao Tang, Yong-Sheng Yu, Guo-Qing Zang
BACKGROUND Chronic hepatitis B virus (HBV) infection is the major cause of hepatocellular carcinoma (HCC). HBV X protein (HBx) plays a crucial role in the development of HCC. Moreover, many tripartite motif (TRIM) family proteins exert diverse biological functions in hepatocarcinogenesis. However, as a novel member of this family, the specific effect of TRIM52 is still largely obscure. In the present study, we investigated the expression and function of TRIM52 in HBV-associated HCC. MATERIAL AND METHODS Fluorescence quantitative polymerase chain reaction (FQ-PCR) was performed to detect the HBV DNA levels in the peripheral blood of HCC patients...
November 1, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29068287/hepatitis-b-virus-x-protein-suppresses-all-trans-retinoic-acid-induced-apoptosis-in-human-hepatocytes-by-repressing-p14-expression-via-dna-methylation
#20
Jung-Hye Choi, Hyerin Jeong, Kyung Lib Jang
All-trans retinoic acid (ATRA), the most biologically active metabolite of vitamin A, is known to activate p14 expression via promoter hypermethylation to induce p53-dependent apoptosis in human hepatocytes. In this study, we found that the oncogenic hepatitis B virus (HBV) X protein (HBx) of HBV, derived from both overexpression and 1.2-mer replicon systems, suppresses ATRA-induced apoptosis in p53-positive human hepatocytes. For this effect, HBx upregulated both protein and enzyme activity levels of DNA methyltransferase 1, 3a and 3b, in the presence of ATRA and thereby inhibited p14 expression via promoter hypermethylation, resulting in inactivation of the p14-mouse double minute 2 pathway and subsequent downregulation of p53 levels...
November 2017: Journal of General Virology
keyword
keyword
31519
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"