keyword
MENU ▼
Read by QxMD icon Read
search

Hbx

keyword
https://www.readbyqxmd.com/read/29760795/capable-infection-of-hepatitis-b-virus-in-diffuse-large-b-cell-lymphoma
#1
Yanchun Wang, Huijie Wang, Shaokun Pan, Tao Hu, Jiabin Shen, Hui Zheng, Suhong Xie, Youhua Xie, Renquan Lu, Lin Guo
Background: Diffuse large B-cell lymphoma (DLBCL) is the most common pathological type of non-Hodgkin lymphoma (NHL). It is strongly correlated to the host immunity and infection status. Aim: This study tested the hypothesis that hepatitis B virus (HBV) infection is also associated with DLBCL. Methods: Clinical analysis of the correlation between DLBCL and HBV infection, detection of HBV in situ of DLBCL tissue, and biological experiments that determined whether HBV infects B lymphocytes were conducted. Results: Our long-term clinical data showed that the positive rate of serum HBV was significantly increased in DLBCL patients (23...
2018: Journal of Cancer
https://www.readbyqxmd.com/read/29752719/hbv-suppresses-zhx2-expression-to-promote-proliferation-of-hcc-through-mir-155-activation
#2
Xiaojia Song, Siyu Tan, Zhuanchang Wu, Leiqi Xu, Zehua Wang, Yong Xu, Tixiao Wang, Chengjiang Gao, Yaoqin Gong, Xiaohong Liang, Lifen Gao, Brett T Spear, Chunhong Ma
Initiation of hepatocellular carcinoma (HCC) by chronic hepatitis B virus (HBV) infection is a complex process that includes both oncogene activation and tumor suppressor inhibition. The HBV X (HBx) protein has an important and complex role in processes leading to HCC. We previously identified the mammalian Zinc fingers and homeoboxes 2 (ZHX2) gene as an HCC-associated tumor suppressor gene. In the present study, we investigated whether the oncogenic properties of HBV and, more specifically, HBx, involved ZHX2 silencing...
May 11, 2018: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/29742265/antiproliferative-effect-of-urolithin-a-the-ellagic-acid-derived-colonic-metabolite-on-hepatocellular-carcinoma-hepg2-2-15-cells-by-targeting-lin28a-let-7a-axis
#3
Zhenpeng Qiu, Junxuan Zhou, Cong Zhang, Ye Cheng, Junjie Hu, Guohua Zheng
An abnormality in the Lin28/let-7a axis is relevant to the progression of hepatitis B virus (HBV)-positive hepatocellular carcinoma (HCC), which could be a novel therapeutic target for this malignant tumor. The present study aimed to investigate the antiproliferative and anti-invasive effects of urolithin A in a stable full-length HBV gene integrated cell line HepG2.2.15 using CCK-8 and transwell assays. The RNA and protein expressions of targets were assessed by quantitative PCR and western blot, respectively...
2018: Brazilian Journal of Medical and Biological Research, Revista Brasileira de Pesquisas Médicas e Biológicas
https://www.readbyqxmd.com/read/29729074/liver-regeneration-accelerates-hepatitis-b-virus-related-tumorigenesis-of-hepatocellular-carcinoma
#4
Chiao-Fang Teng, Hong-Yi Chang, Hung-Wen Tsai, Wen-Chuan Hsieh, Yu-Hao Kuo, Ih-Jen Su, Yih-Jyh Lin
Although partial hepatectomy (PH) to remove tumors provides a potential cure of hepatocellular carcinoma (HCC), long-term survival of hepatitis B virus (HBV)-related HCC patients after PH remains a big challenge. Early recurrence within two years post-PH is associated with the dissemination of primary HCC. However, late recurrence after two years post-PH is supposed due to the de novo or a secondary tumor. Since PH initiates liver regeneration (LR), we hypothesize that LR may accelerate tumorigenesis through activation of pre-existing precancerous lesions in the remaining liver...
May 4, 2018: Molecular Oncology
https://www.readbyqxmd.com/read/29702120/chinese-herbal-extract-su-duxing-had-potent-inhibitory-effects-on-both-wild-type-and-entecavir-resistant-hepatitis-b-virus-hbv-in-vitro-and-effectively-suppressed-hbv-replication-in-mouse-model
#5
Yan Liu, Weiming Yao, Lanlan Si, Jun Hou, Jiabo Wang, Zhihui Xu, Weijie Li, Jianhong Chen, Ruisheng Li, Penggao Li, Lvping Bo, Xiaohe Xiao, Jinchu Lan, Dongping Xu
The present study aimed to investigate anti-HBV effect and major active compounds of Su-duxing, a medicine extracted from Chinese herbs. HBV-replicating cell lines HepG2.2.15 (wild-type) and HepG2. A64 (entecavir-resistant) were used for in vitro test. C57BL/6 mice infected by adeno-associated virus carrying 1.3 mer wild-type HBV genome were used for in vivo test. Inhibitory rates of Su-duxing (10 μg/mL) on HBV replicative intermediate and HBsAg levels were 75.1%, 51.0% in HepG2.2.15 cells and 65.2%, 42...
April 24, 2018: Antiviral Research
https://www.readbyqxmd.com/read/29693167/hbx-promotes-the-proliferative-ability-of-hl%C3%A2-7702-cells-via-the-cox%C3%A2-2-wnt-%C3%AE-%C3%A2-catenin-pathway
#6
Bi-Yun Zheng, Wen-Yu Gao, Xiao-Yun Huang, Li-Ying Lin, Xue-Fen Fang, Zhi-Xin Chen, Xiao-Zhong Wang
Hepatitis B virus X protein (HBx) has been termed a viral oncoprotein, and is involved in the initiation and progression of hepatocellular carcinoma (HCC). Cyclooxygenase‑2 (COX‑2) and β‑catenin have been attributed to the oncogenic activity of HBx in HBV‑associated HCC. The present study aimed to determine whether there is crosstalk between COX‑2 and the Wnt/β‑catenin signaling pathway during HL‑7702‑HBx cell proliferation, and to investigate the associated underlying molecular mechanism...
April 20, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29689279/hepatitis-b-virus-x-protein-elevates-parkin-mediated-mitophagy-through-lon-peptidase-in-starvation
#7
Xiao-Yun Huang, Dan Li, Zhi-Xin Chen, Yue-Hong Huang, Wen-Yu Gao, Bi-Yun Zheng, Xiao-Zhong Wang
Hepatocellular Carcinoma (HCC) is the fifth most prevalent cancer worldwide. Specially, Hepatitis B viurs X protein (HBx) is a leading factor in the progression of Hepatitis B viurs-related HCC. Nutrient-deprived tumor microenvironment also contributes to tumor development. However, the role of HBx in nutrient-deprived HCC has received little investigation. Here, we show that HBx elevates PINK1-Parkin mediating mitophagy in starvation. HBx not only increases the PINK1/Parkin gene expression but also accelerates Parkin recruitment to partial mitochondria...
April 21, 2018: Experimental Cell Research
https://www.readbyqxmd.com/read/29624717/sirt3-restricts-hbv-transcription-and-replication-via-epigenetic-regulation-of-cccdna-involving-suv39h1-and-setd1a-histone-methyltransferases
#8
Ji-Hua Ren, Jie-Li Hu, Sheng-Tao Cheng, Hai-Bo Yu, Vincent Kam Wai Wong, Betty Yuen Kwan Law, Yong-Feng Yang, Ying Huang, Yi Liu, Wei-Xian Chen, Xue-Fei Cai, Hua Tang, Yuan Hu, Wen-Lu Zhang, Xiang Liu, Quan-Xin Long, Li Zhou, Na-Na Tao, Hong-Zhong Zhou, Qiu-Xia Yang, Fang Ren, Lin He, Rui Gong, Ai-Long Huang, Juan Chen
Hepatitis B virus (HBV) infection remains a major health problem worldwide. Maintenance of the covalently closed circular DNA (cccDNA) which serves as a template for HBV RNA transcription is responsible for the failure of eradicating chronic HBV during current antiviral therapy. cccDNA is assembled with cellular histone proteins into chromatin, but little is known about the regulation of HBV chromatin by histone posttranslational modifications. In this study, we identified SIRT3 as a host factor restricting HBV transcription and replication by screening seven members of Sirtuin family which is the class III histone deacetylase...
April 6, 2018: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
https://www.readbyqxmd.com/read/29614505/hbv-facilitated-hepatocellular-carcinoma-cells-proliferation-by-up-regulating-angiogenin-expression-through-il-6
#9
Xiaotang Zhou, Fan Yang, Ying Yang, Ying Hu, Weixia Liu, Chunhong Huang, Shuping Li, Zhi Chen
BACKGROUND/AIMS: Patients with hepatitis B virus (HBV) infection are at a high risk of developing hepatocellular carcinoma (HCC). In this study, we aim to investigate the roles of HBV on angiogenin (ANG), as well as the effects on cell proliferation in presence of ANG down-regulation. METHODS: Serum ANG was determined by ELISA. The expression of ANG mRNA and protein in HCC cell lines with or without HBV/HBx were determined. Western blot and ELISA were conducted to determine the effects of HBV/HBx on IL-6 expression...
March 26, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29611800/hepatitis-b-virus-x-protein-activates-proteasomal-activator-28-gamma-expression-via-upregulation-of-p53-levels-to-stimulate-virus-replication
#10
Sujeong Yeom, Hyerin Jeong, Soo Shin Kim, Kyung Lib Jang
Proteasomal activator gamma (PA28γ), frequently overexpressed in hepatocellular carcinoma, is believed to play important roles in tumourigenesis. However, the underlying mechanism of PA28γ overexpression and its possible roles in hepatitis B virus (HBV) replication are largely unknown. In the present study, we found that hepatitis B virus X protein (HBx) activates PA28γ expression by upregulating p53 levels in human hepatoma cells. The elevated PA28γ levels in turn repressed seven in absentia homologue 1 expression via downregulation of p53 levels, thereby inhibiting ubiquitin-dependent proteasomal degradation of HBx, which ultimately led to upregulation of HBx levels...
April 3, 2018: Journal of General Virology
https://www.readbyqxmd.com/read/29604207/hepatitis-b-virus-x-protein-promotes-proliferation-of-hepatocellular-carcinoma-cells-by-upregulating-mir-181b-via-targeting-ing5
#11
Xuhua Xie, Xiaopei Xu, Changyu Sun, Zujiang Yu
Hepatitis B virus X protein (HBx) played a key role in the development of hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC). Emerging evidence has demonstrated that miR-181b and the inhibitor of growth protein 5 (ING5) participated in the pathophysiological process. However, the regulatory mechanism of HBx remained unknown. The expression of miR-181b and ING5 in HCC tissues and cell lines were examined using quantitative real-time PCR (qRT-PCR) and Western blotting. Cell viability was determined using MTT method following HCC cell lines transfection...
March 1, 2018: Biological Chemistry
https://www.readbyqxmd.com/read/29600594/hbx-protein-promotes-lasp-1-expression-through-activation-of-c-jun-in-human-hepatoma-cells
#12
Hongjuan You, Fanyun Kong, Kai Zhou, Xiao Wei, Lei Hu, Wei Hu, Wenya Luo, Yanbo Kou, Xiaomei Liu, Xi Chen, Kuiyang Zheng, Renxian Tang
LIM and SH3 domain protein 1 (LASP-1) is known to participate in the progression of hepatocellular carcinoma (HCC). We previously showed that ectopic expression of hepatitis B virus (HBV) X protein (HBX) enhanced the expression of LASP-1, which promoted proliferation and migration of HCC cells. Here, we further demonstrated the molecular mechanism underlying upregulation of LASP-1, mediated by HBX, in HBV-infected HCC cells. Through a luciferase activity assay, we discovered that the LASP-1 promoter region regulated by HBX contained an AP-1 binding element in human hepatoma cells...
March 30, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29563998/pdk1-wnk1-signaling-is-affected-by-hbx-and-involved-in-the-viability-and-metastasis-of-hepatic-cells
#13
Chaoying Li, Cong Lin, Xianling Cong, Ying Jiang
Hepatitis B virus (HBV)-encoded X antigen (HBx) contributes to the development of hepatocellular carcinoma (HCC). Although HBx has been implicated in the progression of HCC, its precise function in HBV-associated HCC remains unclear. In the present study, HBx affected 3-phosphoinositide-dependent protein kinase-1 (PDK1) and with-no-lysine (K) kinase (WNK1) signaling, which was identified to be involved in the viability and metastasis of hepatic cells. The phosphorylation of WNK1 was decreased when the hepatic cells were treated with a PDK1 inhibitor...
April 2018: Oncology Letters
https://www.readbyqxmd.com/read/29557344/broad-spectrum-antiviral-activity-of-the-deubiquitinase-inhibitor-hbx-against-human-adenoviruses
#14
Karin Kosulin, Elena Lam, Albert Heim, Thomas Dobner, Estefanía Rodríguez
BACKGROUND: Human adenoviral (HAdV) infections are usually mild and self-limited, however, some infections from species A, B, C, D and E, can cause severe illnesses, which have raised public health concerns over the past few years. Current available antiviral therapies have limited efficacy and severe toxicity; therefore, finding new targets for specific anti-adenoviral drug design is urgently needed. Our previous work showed that the small molecule compound, HBX, inhibits HAdV type 5 (species C, HAdV-C5) replication and oncogenic transformation through inhibition of the cellular pro-viral factor ubiquitin-specific protease 7 (USP7)...
March 20, 2018: Antiviral Therapy
https://www.readbyqxmd.com/read/29555949/delta-like-3-is-silenced-by-hbx-via-histone-acetylation-in-hbv-associated-hccs
#15
Hiroki Hamamoto, Kentaro Maemura, Kentaro Matsuo, Kohei Taniguchi, Yoshihisa Tanaka, Sugiko Futaki, Atsushi Takeshita, Akira Asai, Michihiro Hayashi, Yoshinobu Hirose, Yoichi Kondo, Kazuhisa Uchiyama
Hepatocellular carcinoma (HCC) is a common malignant tumor with poor prognosis. We previously showed that expression of Delta-like 3 (DLL3), a member of the family of Delta/Serrate/Lag2 ligands for the Notch receptor, is silenced by aberrant DNA methylation and that overexpression of DLL3 in an HCC cell line induces cellular apoptosis. However, how DLL3 expression is regulated during hepatocarcinogenesis is still unclear. Here, we show that silencing of DLL3 during hepatocarcinogenesis is closely related to viral infection, especially hepatitis B virus (HBV) infection (p = 0...
March 19, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29552166/hepatitis-b-virus-upregulates-gp73-expression-by-activating-the-hif-2%C3%AE-signaling-pathway
#16
Sheng-Li Yang, Cui Zeng, Xiefan Fang, Qian-Jin He, Li-Ping Liu, Shi-Yun Bao, Xiaoli Pan, Zhi-Fan Xiong
Golgi Protein 73 (GP73) is a newly identified diagnostic and prognostic marker for liver cancer. GP73 is highly expressed in liver cancer tissues, however, the mechanism of its overexpression in tumors remains unknown. In the present study, the effect of hepatitis B virus (HBV) on GP73 expression was investigated in HepG2 cells, which are negative for HBV, and in HepG2.2.12 cells, which are integrated with HBV, using reverse transcription-quantitative polymerase chain reaction and western blot analysis. In addition, the cells were transfected with plasmid constructs overexpressing hepatitis B virus protein X (HBx), hypoxia-inducible factor (HIF)-1α, or HIF-2α in order to examine their roles in GP73 expression...
April 2018: Oncology Letters
https://www.readbyqxmd.com/read/29523836/type-iii-interferon-induced-cbf%C3%AE-inhibits-hbv-replication-by-hijacking-hbx
#17
Fengchao Xu, Hongxiao Song, Qingfei Xiao, Na Li, Hong Zhang, Genhong Cheng, Guangyun Tan
Hepatitis B virus (HBV) and its associated chronic infection remain serious health threats worldwide. However, there is still no impactful approach for clinical treatment of hepatitis B patients. Therefore, developing a better understanding of the interactions between HBV and its host is particularly important. HBV infection has been reported to induce type-III but not type-I or type-II interferon (IFN). In this study, we identified CBFβ, an HIV enhancer, as an HBV restriction factor that is specifically induced by type-III IFN in the early stages of HBV infection...
March 9, 2018: Cellular & Molecular Immunology
https://www.readbyqxmd.com/read/29475611/hepatitis-b-virus-x-protein-represses-lkb1-expression-to-promote-tumor-progression-and-poor-postoperative-outcome-in-hepatocellular-carcinoma
#18
Cheng-Chung Wu, De-Wei Wu, Ying-Yu Lin, Po-Lin Lin, Huei Lee
BACKGROUND: Hepatitis B virus X (HBx) protein plays critical roles in hepatitis B virus (HBV)-associated hepatocellular tumorigenesis through different molecular mechanisms, including inactivation of p53, a key transcription factor of liver kinase B1 (LKB1). We hypothesized that p53 inactivation by HBx protein could decrease LKB1 expression, thereby promoting tumor progression and poor outcomes in patients with HBV-associated hepatocellular carcinoma. METHODS: Manipulation strategies for HBx protein and/or p53 were used to verify that loss of LKB1 could promote colony formation and invasiveness in HepG2 and Hep3B cells...
May 2018: Surgery
https://www.readbyqxmd.com/read/29472430/in-vivo-phosphoproteome-analysis-reveals-kinome-reprogramming-in-hepatocellular-carcinoma
#19
Liangliang Ren, Chaoying Li, Youliang Wang, Yan Teng, Huichuan Sun, Baocai Xing, Xiao Yang, Ying Jiang, Fuchu He
Aberrant kinases contribute to cancer survival and proliferation. Here, we quantitatively characterized phosphoproteomic changes in an HBx-transgenic mouse model of hepatocellular carcinoma (HCC) using high-resolution mass spectrometry, profiled 22,539 phosphorylation sites on 5,431 proteins. Utilizing a strategy to interpret kinase-substrate relations in HCC and to uncover predominant kinases in tumors, our results, revealed elevated kinase activities of Src family kinases (SFKs), PKCs, MAPKs, and ROCK2 in HCC, representatives of which were further validated in cell models and clinical HBV-positive HCC samples...
February 22, 2018: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/29454995/role-of-hbx-in-hepatitis-b-virus-persistence-and-its-therapeutic-implications
#20
REVIEW
Betty L Slagle, Michael J Bouchard
Chronic hepatitis B virus infection is a significant risk factor for cirrhosis and hepatocellular carcinoma. The HBx protein is required for virus replication, but the lack of robust infection models has hindered our understanding of HBx functions that could be targeted for antiviral purposes. We briefly review three properties of HBx: its binding to DDB1 and its regulation of cell survival and metabolism, to illustrate how a single viral protein can have multiple effects in a cell. We propose that different functions of HBx are needed, depending on the changing hepatocyte environment encountered during a chronic virus infection, and that these functions might serve as novel therapeutic targets for inhibiting hepatitis B virus replication and the development of associated diseases...
February 15, 2018: Current Opinion in Virology
keyword
keyword
31519
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"