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https://www.readbyqxmd.com/read/27920474/hepatitis-b-virus-upregulates-host-expression-of-%C3%AE-1-2-mannosidases-via-the-ppar%C3%AE-pathway
#1
Song Hu, Li-Bin Jiang, Xiao-Jing Zou, Wei Yi, De-Ying Tian
AIM: To assess the effects of hepatitis B virus (HBV) on the expression of host α-1,2-mannosidases and determine the underlying mechanisms. METHODS: We measured the expression levels of MAN1A1, MAN1A2, MAN1B1, and MAN1C1 in cell lines HepG2.2.15, HepN10, HepAD38 and HepG2 by Western blot. Viral antigens (HBsAg and HBeAg) in the culture medium were measured using the chemiluminescence method. HBV DNA quantification assays were performed using a commercial real-time PCR kit...
November 21, 2016: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/27909336/c-terminal-truncated-hbv-x-promotes-hepato-oncogenesis-through-inhibition-of-tumor-suppressive-%C3%AE-catenin-bambi-signaling
#2
Seok Lee, Mi-Jin Lee, Jun Zhang, Goung-Ran Yu, Dae-Ghon Kim
C-terminal-truncated hepatitis B virus (HBV) X (HBx) (ctHBX) is frequently detected in hepatocellular carcinoma (HCC) through HBV integration into the host genome. However, the molecular mechanisms underlying ctHBx-associated oncogenic signaling have not yet been clarified. To elucidate the biological role of ctHBx in hepato-oncogenesis, we functionally analyzed ctHBx-mediated regulation of the activin membrane-bound inhibitor bone morphogenetic protein and activin membrane-bound inhibitor (BAMBI) through transforming growth factor-β (TGF-β) or β-catenin (CTNNB1) in HCC cells and in an animal model, and we compared its role to that of the full-length HBx protein...
December 2, 2016: Experimental & Molecular Medicine
https://www.readbyqxmd.com/read/27902472/molecular-mechanism-of-hepatitis-b-virus-hbv-on-suppression-of-raf-kinase-inhibitor-protein-rkip-expression
#3
Xiao-Ke Cheng, Guo-Zheng Yu, Xiao-Dong Li, Xue-Qun Ren
Raf kinase inhibitor protein (RKIP) has been shown to be a suppressor of the mitogen-activated protein kinase pathway and is reported to be involved in human malignancy. However, the molecular mechanism of hepatitis B virus (HBV) in regulating RKIP expression is not yet clarified. In this study, we compared RKIP expression in 107 pairs of matched liver cancer and adjacent non-cancerous liver tissues. Among seven HBV-encoded proteins, we found HBV X (HBX) protein could significantly inhibit the expression level of RKIP, indicating that HBV could suppress RKIP expression through regulating HBX...
November 25, 2016: Oncotarget
https://www.readbyqxmd.com/read/27883059/hepatic-satb1-induces-paracrine-activation-of-hepatic-stellate-cells-and-is-upregulated-by-hbx
#4
Jin Gong, Wei Tu, Jian Han, Jiayi He, Jingmei Liu, Ping Han, Yunwu Wang, Mengke Li, Mei Liu, Jiazhi Liao, Dean Tian
Chronic hepatitis B virus (HBV) infection is a major cause of chronic liver diseases, but its involvement in hepatic fibrogenesis remains unclear. Special AT-rich binding protein 1 (SATB1) has been implicated in reprogramming chromatin organization and transcription profiles in many cancers and non-cancer-related conditions. We found that hepatic SATB1 expression was significantly up-regulated in fibrotic tissues from chronic hepatitis B virus (HBV)-infected patients and HBV transgenic (HBV-Tg) mouse model...
November 24, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27880978/defining-the-relationship-between-farsenoid-x-receptor-hepatitis-b-virus-x-protein-and-hcc-it-s-complicated
#5
EDITORIAL
Lindsey Kennedy, Heather Francis
The relationship between hepatitis B virus X protein (HBx), farsenoid X receptor (FXR) and hepatocellular carcinoma (HCC) is a complicated one in that we have a viral protein interaction that can drive tumorigenesis or inhibit HCC depending upon transactivation of full-length or truncated HBx. In the current article the authors have elegantly described a system of HBx-FXR interaction that demonstrates inhibition of HCC tumor growth via activation of full-length HBx. The paper employs both in vivo and in vitro studies including using FXR knockout mice crossed with HBx induced mice...
November 23, 2016: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
https://www.readbyqxmd.com/read/27864147/host-factor-prpf31-is-involved-in-cccdna-production-in-hbv-replicating-cells
#6
Wataru Kinoshita, Naoki Ogura, Koichi Watashi, Takaji Wakita
Hepatitis B virus (HBV) covalently closed circular DNA (cccDNA) plays a central role in chronic HBV infection and replication, and is an important factor for HBV surface antigen loss indicating the endpoint of HBV treatment. However, there is a known problem that current anti-HBV drugs, including interferons and nucleos(t)ide analogues, reduce HBV replication but have a little or no effect on reducing cccDNA. Therefore, the development of new therapeutic agents is necessary to eradicate cccDNA. In this study, we identified pre-mRNA processing factor 31 (PRPF31) by siRNA screening as a factor associated with cccDNA...
November 15, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/27821177/hepatitis-b-virus-x-protein-promotes-interleukin-7-receptor-expression-via-nf-%C3%AE%C2%BAb-and-notch1-pathway-to-facilitate-proliferation-and-migration-of-hepatitis-b-virus-related-hepatoma-cells
#7
Fanyun Kong, Wei Hu, Kai Zhou, Xiao Wei, Yanbo Kou, Hongjuan You, Kuiyang Zheng, Renxian Tang
BACKGROUND: Interleukin-7 receptor (IL-7R) is involved in the abnormal function of solid tumors, but the role and regulatory mechanisms of IL-7R in HBV-related hepatocellular carcinoma (HCC) are still unclear. METHODS: Gene and protein expression levels of IL-7R were examined in hepatoma cells transfected with hepatitis B virus (HBV) plasmids and in hepatoma cells transfected with the multifunctional nonstructural protein X (HBX). The expression of HBX and IL-7R was measured by immunohistochemical analysis in HBV-related HCC tissues...
November 7, 2016: Journal of Experimental & Clinical Cancer Research: CR
https://www.readbyqxmd.com/read/27819342/minicircle-hbv-cccdna-with-a-gaussia-luciferase-reporter-for-investigating-hbv-cccdna-biology-and-developing-cccdna-targeting-drugs
#8
Feng Li, Liang Cheng, Christopher M Murphy, Natalia J Reszka-Blanco, Yaxu Wu, Liqun Chi, Jianming Hu, Lishan Su
Chronic Hepatitis B Virus (HBV) infection is generally not curable with current anti-viral drugs. Virus rebounds after stopping treatment from the stable HBV covalently-closed-circular DNA (cccDNA). The development of drugs that directly target cccDNA is hampered by the lack of robust HBV cccDNA models. We report here a novel HBV cccDNA technology that will meet the need. We engineered a minicircle HBV cccDNA with a Gaussia Luciferase reporter (mcHBV-GLuc cccDNA), which serves as a surrogate to measure cccDNA activity...
November 7, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27798869/ursolic-acid-suppresses-hepatitis-b-virus-x-protein-mediated-autophagy-and-chemotherapeutic-drug-resistance
#9
Ching-Dong Chang, Ping-Yuan Lin, Jue-Liang Hsu, Wen-Ling Shih
Hepatitis B virus X (HBx) protein is a multifunctional oncoprotein that affects diverse cell activities via regulation of various host cell signaling pathways. The current investigation demonstrated that ursolic acid (UA), a pentacyclic triterpenoid, protected hepatoma cells and reduced HBx-mediated autophagy through modulation of Ras homolog gene family member A (RhoA). Low-level ectopic HBx expression in Huh7 cells induced more significant autophagosome formation than high-level HBx expression. HBx activated beclin-1 promoter and enhanced the beclin-1 protein expression under low HBx expression...
October 2016: Anticancer Research
https://www.readbyqxmd.com/read/27793933/apoptosis-of-hepatitis-b-virus-expressing-liver-tumor-cells-induced-by-a-high-concentration-of-nucleos-t-ide-analogue
#10
Eunyoung Tak, Shin Hwang, Han Chu Lee, Gi-Young Ko, Chul-Soo Ahn, Young-In Yoon, Young-Suk Lim, Dae-Young Jun, Ki-Hun Kim, Gi-Won Song, Deog-Bok Moon, Baek-Yeol Ryoo, Nayoung Kim, Sung-Gyu Lee
BACKGROUND/AIM: We investigated the expression of hepatitis B virus (HBV) covalently closed circular DNA (cccDNA) and HBV X protein (HBx) in human hepatocellular carcinoma (HCC) and evaluated the effect of high-concentration nucleos(t)ide analogs (NUCs) on liver tumor cell lines. MATERIALS AND METHODS: This study consisted of three parts: part I used human blood and non-tumor liver tissues; part II used human HCC and adjacent liver tissues; and part III used an HBV-expressing liver tumor cell line...
November 2016: Anticancer Research
https://www.readbyqxmd.com/read/27780730/mir-203a-is-involved-in-hbx-induced-inflammation-by-targeting-rap1a
#11
AiRong Wu, Huo Chen, ChunFang Xu, Ji Zhou, Si Chen, YuQi Shi, Jie Xu, JianHe Gan, JinPing Zhang
Hepatitis B virus (HBV) causes acute and chronic hepatitis, and is one of the major causes of cirrhosis and hepatocellular carcinoma. Accumulating evidence suggests that inflammation is the key factor for liver cirrhosis and hepatocellular carcinoma. MicroRNAs play important roles in many biological processes. Here, we aim to explore the function of microRNAs in the HBX-induced inflammation. First, microarray experiment showed that HBV(+) liver samples expressed higher level of miR-203a compared to HBV(-) liver samples...
October 22, 2016: Experimental Cell Research
https://www.readbyqxmd.com/read/27775586/hepatitis-b-virus-protein-x-induces-degradation-of-talin-1
#12
Maarten A A van de Klundert, Maartje van den Biggelaar, Neeltje A Kootstra, Hans L Zaaijer
In the infected human hepatocyte, expression of the hepatitis B virus (HBV) accessory protein X (HBx) is essential to maintain viral replication in vivo. HBx critically interacts with the host damaged DNA binding protein 1 (DDB1) and the associated ubiquitin ligase machinery, suggesting that HBx functions by inducing the degradation of host proteins. To identify such host proteins, we systematically analyzed the HBx interactome. One HBx interacting protein, talin-1 (TLN1), was proteasomally degraded upon HBx expression...
October 19, 2016: Viruses
https://www.readbyqxmd.com/read/27768594/hepatitis-b-virus-x-protein-influences-enrichment-profiles-of-h3k9me3-on-promoter-regions-in-human-hepatoma-cell-lines
#13
Di-Yi Wang, Shu-Hong An, Lei Liu, Shan-Shan Bai, Kai-Xiang Wu, Rong Zhu, Zhao-Jin Wang
We previously showed that hepatitis B virus (HBV) X protein (HBx) could promote the trimethylation of histone H3 lysine 9 (H3K9me3) to repress tumor suppressor genes in hepatocellular carcinoma (HCC). In this work, we analyze 23,148 human promoters using ChIP-chip to determine the effects of HBx on H3K9me3 enrichments in hepatoma cells with transfection of HBx-expressing plasmid. Immunohistochemistry for HBx and H3K9me3 was performed in 21 cases of HBV-associated HCC tissues. We identified that H3K9me3 immunoreactivity was significantly correlated with HBx staining in HCC tissues...
October 19, 2016: Oncotarget
https://www.readbyqxmd.com/read/27748878/interleukin%C3%A2-33%C3%A2-induced-immune-tolerance-is-associated-with-the-imbalance-of-memory-and-na%C3%A3-ve-t%C3%A2-lymphocyte-subsets
#14
Xu Shi, Ying Tang, Xiguang Sun, Yufei Liu, Ying Sun, Munan Sun, Yanfang Jiang, Yulin Li
The current study aimed to investigate the distribution of memory and naïve T cell (TN) subsets in hepatitis B virus (HBV)‑infected patients at different immune stages and investigate the effect of interleukin 33 (IL‑33) on the regulation of the T‑cell subsets. The distributions of memory and naïve T cells were detected by flow cytometry. ELISA was conducted to assess the levels of IL‑4, IL‑5, IL‑10, IL‑12, interferon γ and tumor necrosis factor α. The expression levels of IL‑33 and HBV x protein (HBx) were measured by reverse transcription‑quantitative polymerase chain reaction and western blot analysis, respectively...
October 13, 2016: Molecular Medicine Reports
https://www.readbyqxmd.com/read/27740879/hepatitis-b-virus-triggered-autophagy-targets-tnfrsf10b-death-receptor-5-for-degradation-to-limit-tnfsf10-trail-response
#15
Gu-Choul Shin, Hong Seok Kang, Ah Ram Lee, Kyun-Hwan Kim
Death receptors of TNFSF10/TRAIL (tumor necrosis factor superfamily member 10) contribute to immune surveillance against virus-infected or transformed cells by promoting apoptosis. Many viruses evade antiviral immunity by modulating TNFSF10 receptor signaling, leading to persistent infection. Here, we report that hepatitis B virus (HBV) X protein (HBx) restricts TNFSF10 receptor signaling via macroautophagy/autophagy-mediated degradation of TNFRSF10B/DR5, a TNFSF10 death receptor, and thus permits survival of virus-infected cells...
October 14, 2016: Autophagy
https://www.readbyqxmd.com/read/27723845/hbx-and-sp1-upregulate-dkk1-expression
#16
Hong Peng, Yongguo Li, Yunzhi Liu, Jingnan Zhang, Ke Chen, Ailong Huang, Hua Tang
Numerous evidences suggested that the hepatitis B virus (HBV) was recognized as an important factor in the development of hepatocellular carcinoma (HCC). Dickkopf-1 (DKK1) recently was reported to be involved in the progress of HCC. HBV may regulate DKK1 expression in hematoma carcinogenesis. Here, we demonstrated that HBV could regulate DKK1 promoter activity which resulted in upregulation of its mRNA and protein expression in several HBV existing cell lines, and HBx played a prominent role in this process...
October 10, 2016: Acta Biochimica Polonica
https://www.readbyqxmd.com/read/27708347/humic-acid-inhibits-hbv-induced-autophagosome-formation-and-induces-apoptosis-in-hbv-transfected-hep-g2-cells
#17
Kishor Pant, Ajay K Yadav, Parul Gupta, Abhishek Singh Rathore, Baibaswata Nayak, Senthil K Venugopal
Hepatitis B Virus (HBV) utilizes several mechanisms to survive in the host cells and one of the main pathways being autophagosome formation. Humic acid (HA), one of the major components of Mineral pitch, is an Ayurvedic medicinal food, commonly used by the people of the Himalayan regions of Nepal and India for various body ailments. We hypothesized that HA could induce cell death and inhibit HBV-induced autophagy in hepatic cells. Incubation of Hep G2.2.1.5 cells (HepG2 cells stably expressing HBV) with HA (100 μM) inhibited both cell proliferation and autophagosome formation significantly, while apoptosis induction was enhanced...
October 6, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27708241/hepatitis-b-virus-x-induces-inflammation-and-cancer-in-mice-liver-through-dysregulation-of-cytoskeletal-remodeling-and-lipid-metabolism
#18
Zhongwei Xu, Linghui Zhai, Tailong Yi, Huiying Gao, Fengxu Fan, Yanchang Li, Youliang Wang, Ning Li, Xiaohua Xing, Na Su, Feilin Wu, Lei Chang, Xiuli Chen, Erhei Dai, Chao Zhao, Xiao Yang, Chunping Cui, Ping Xu
Hepatitis B virus X protein (HBx) participates in the occurrence and development processes of hepatocellular carcinoma (HCC) as a multifunctional regulation factor. However, the underlying molecular mechanism remains obscure. Here, we describe the use of p21HBx/+ mouse and SILAM (Stable Isotope Labeling in Mammals) strategy to define the pathological mechanisms for the occurrence and development of HBx induced liver cancer. We systematically compared a series of proteome samples from regular mice, 12- and 24-month old p21HBx/+ mice representing the inflammation and HCC stages of liver disease respectively and their nontransgenic wild-type (WT) littermates...
September 30, 2016: Oncotarget
https://www.readbyqxmd.com/read/27702890/hypoxia-activated-cytotoxic-agent-tirapazamine-enhances-hepatic-artery-ligation-induced-killing-of-liver-tumor-in-hbx-transgenic-mice
#19
Wei-Hsiang Lin, Shiou-Hwei Yeh, Kun-Huei Yeh, Kai-Wei Chen, Ya-Wen Cheng, Tung-Hung Su, Ping Jao, Lin-Chun Ni, Pei-Jer Chen, Ding-Shinn Chen
Transarterial chemoembolization (TACE) is the main treatment for intermediate stage hepatocellular carcinoma (HCC) with Barcelona Clinic Liver Cancer classification because of its exclusive arterial blood supply. Although TACE achieves substantial necrosis of the tumor, complete tumor necrosis is uncommon, and the residual tumor generally rapidly recurs. We combined tirapazamine (TPZ), a hypoxia-activated cytotoxic agent, with hepatic artery ligation (HAL), which recapitulates transarterial embolization in mouse models, to enhance the efficacy of TACE...
October 18, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/27701872/does-hepatitis-b-virus-infection-cause-breast-cancer
#20
Vishnu Prasad Adhikari, Lin-Jie Lu, Ling-Quan Kong
Lifestyle and family history are two of the most important risk factors for breast cancer (BC). However, these risk factors cannot explain the differences in the incidence and early BC onset among Chinese females compared to their western counterparts. We propose in this hypothesis the potential mechanism of indirect oncogenesis of hepatitis B virus (HBV) in causing BC through its persistence as occult infection and continuous replication with long term subtle liver damage. Estrogen is mainly deactivated in the liver and long term necro-inflammatory damage to liver may result in persistent high level of estrogen, which is a dominant risk factor for BC...
September 12, 2016: Chinese Clinical Oncology
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