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https://www.readbyqxmd.com/read/29133292/intracellular-hepatitis-b-virus-increases-hepatic-cholesterol-deposition-in-alcoholic-fatty-liver-via-hepatitis-b-core-protein
#1
Ya-Qi Wang, Ting Wu, Dan-Qing Hu, Xin-Xin Weng, Xiao-Jing Wang, Pei-Jer Chen, Xiao-Ping Luo, Hong-Wu Wang, Qin Ning
Hepatits B virus (HBV) infection is a prevalent infectious disease with serious outcomes like chronic and acute hepatitis, cirrhosis and hepatocellular carcinoma. However, the metabolic alteration by HBV is rarely taken into concerns. With the high prevalence of alcohol consumption and chronic HBV infection, their overlap is assumed as an increasing latent hazard although without exact calculation, moreover, the impact of chronic alcoholic consumption combined with HBV on cholesterol metabolism is unknown. Six-week-old male FVB/Ncrl mice were hydrodynamically injected with a pGEM-4Z-1...
November 13, 2017: Journal of Lipid Research
https://www.readbyqxmd.com/read/29114584/expression-purification-and-characterization-of-hepatitis-b-virus-x-protein-bh3-like-motif-linker-bcl-xl-fusion-protein-for-structural-studies
#2
Hideki Kusunoki, Toshiyuki Tanaka, Toshiyuki Kohno, Hirokazu Kimura, Kazuo Hosoda, Kaori Wakamatsu, Isao Hamaguchi
Hepatitis B virus X protein (HBx) is a multifunctional protein that interacts directly with many host proteins. For example, HBx interacts with anti-apoptotic proteins, Bcl-2 and Bcl-xL, through its BH3-like motif, which leads to elevated cytosolic calcium levels, efficient viral DNA replication and the induction of apoptosis. To facilitate sample preparation and perform detailed structural characterization of the complex between HBx and Bcl-xL, we designed and purified a recombinant HBx BH3-like motif-linker-Bcl-xL fusion protein produced in E...
March 2017: Biochemistry and Biophysics Reports
https://www.readbyqxmd.com/read/29103765/downregulation-of-mir-200a-3p-induced-by-hepatitis-b-virus-x-hbx-protein-promotes-cell-proliferation-and-invasion-in-hbv-infection-associated-hepatocarcinoma
#3
Taiyang Shi, Qinfang Hua, Zhipeng Ma, Qijun Lv
BACKGROUND: Hepatitis B Virus X (HBx) Protein encoded by HBV is believed to be the major player in the process of HBV-induced oncogenesis. Ectopic expression of miR-200a-3p was reported to be associated with diverse tumorigenesis. This study aimed to better understand the role of miR-200a-3p and its correlation with HBx in HBV-induced hepatocellular carcinoma (HCC). METHODS: In this report, we examined the gene expression using quantitative RT-PCR and protein expression using Western blotting analysis...
October 26, 2017: Pathology, Research and Practice
https://www.readbyqxmd.com/read/29089476/tripartite-motif-containing-52-trim52-promotes-cell-proliferation-in-hepatitis-b-virus-associated-hepatocellular-carcinoma
#4
Yi Zhang, Shan-Shan Wu, Xiao-Hua Chen, Zheng-Hao Tang, Yong-Sheng Yu, Guo-Qing Zang
BACKGROUND Chronic hepatitis B virus (HBV) infection is the major cause of hepatocellular carcinoma (HCC). HBV X protein (HBx) plays a crucial role in the development of HCC. Moreover, many tripartite motif (TRIM) family proteins exert diverse biological functions in hepatocarcinogenesis. However, as a novel member of this family, the specific effect of TRIM52 is still largely obscure. In the present study, we investigated the expression and function of TRIM52 in HBV-associated HCC. MATERIAL AND METHODS Fluorescence quantitative polymerase chain reaction (FQ-PCR) was performed to detect the HBV DNA levels in the peripheral blood of HCC patients...
November 1, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29068287/hepatitis-b-virus-x-protein-suppresses-all-trans-retinoic-acid-induced-apoptosis-in-human-hepatocytes-by-repressing-p14-expression-via-dna-methylation
#5
Jung-Hye Choi, Hyerin Jeong, Kyung Lib Jang
All-trans retinoic acid (ATRA), the most biologically active metabolite of vitamin A, is known to activate p14 expression via promoter hypermethylation to induce p53-dependent apoptosis in human hepatocytes. In this study, we found that the oncogenic hepatitis B virus (HBV) X protein (HBx) of HBV, derived from both overexpression and 1.2-mer replicon systems, suppresses ATRA-induced apoptosis in p53-positive human hepatocytes. For this effect, HBx upregulated both protein and enzyme activity levels of DNA methyltransferase 1, 3a and 3b, in the presence of ATRA and thereby inhibited p14 expression via promoter hypermethylation, resulting in inactivation of the p14-mouse double minute 2 pathway and subsequent downregulation of p53 levels...
November 2017: Journal of General Virology
https://www.readbyqxmd.com/read/29065733/hepatitis-b-core-protein-as-a-therapeutic-target
#6
Lung-Yi Mak, Danny Ka-Ho Wong, Wai-Kay Seto, Ching-Lung Lai, Man Fung Yuen
Chronic hepatitis B virus (HBV) infection is difficult to cure, due to the presence of covalently-closed-circular DNA and virus-mediated blunting of host immune response. Existing therapies with nucleos(t)ide analogue or pegylated-interferon are not sufficient to achieve a high rate of HBV surface antigen seroclearance, a more desirable treatment outcome. Novel therapeutic agents targeting alternative viral replication steps are being developed. In this review, we will discuss the hepatitis B core antigen (HBcAg) as a therapeutic target...
December 2017: Expert Opinion on Therapeutic Targets
https://www.readbyqxmd.com/read/29052908/hbxag-suppresses-cell-apoptosis-and-promotes-the-secretion-of-placental-hormones-in-human-placental-trophoblasts-via-activation-of-the-egfr-akt-pathway
#7
Weimin Wang, Guiqin Bai, Yuting Zhang, Ting Zhang, Chen Li, Yongxing Yuan, Sixue Liu, Caili Wang
The aim of this study is to investigate the role of Hepatitis B virus x (HBx) in the growth and secretion of human placental trophoblasts. Firstly, placenta tissues were collected from pregnant HBV carriers with various viral loads. The results of immunohistochemical technique showed that the HBx protein and pEGFR protein levels were both markedly increased with the viral load elevation. Then, a placental trophoblast cell strain (JEG-3-HBx), which stablly expressed HBx mRNA and protein, was established with the pcDNA-HBx transfection followed by the G418 selection...
October 20, 2017: Cell Biology International
https://www.readbyqxmd.com/read/29052129/hepatitis-b-virus-associated-hepatocellular-carcinoma
#8
Youhua Xie
Liver cancer is the fifth most common cancer worldwide in men and the ninth in women. It is also the second most common cause of cancer mortality. Hepatocellular carcinoma (HCC) is the most common type of liver cancer. About 350 million people globally are chronically infected with HBV. Chronic hepatitis B virus (HBV) infection accounts for at least 50% cases of HCC worldwide. Other non-HBV factors may increase HCC risk among persons with chronic HBV infection. Both indirect and direct mechanisms are involved in HCC oncogenesis by HBV...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/29048612/regulation-of-erk-and-akt-pathways-by-hepatitis%C3%A2-b-virus%C3%A2-x-protein-via-the-notch1-pathway-in-hepatocellular-carcinoma
#9
Bo Liao, Honghao Zhou, Huifang Liang, Changhai Li
Hepatitis B virus (HBV) is the dominant risk factor for hepatocellular carcinoma (HCC). HBV X protein (HBx) plays crucial roles in HCC carcinogenesis. HBx interferes with several signaling pathways including the Notch1 pathway in HCC. In this study, we found that Notch1 was highly expressed in HCC, especially in large HCCs. Notch1 and HBx co-localized in HCC and their levels were positively correlated with each other. Notch1 expression was more elevated in HepG2.2.15 cells than that in HepG2 cells. HBx activated the Notch1 pathway in HepG2...
September 15, 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/29046350/host-transcription-factor-speckled-110kda-sp110-a-nuclear-body-protein-is-hijacked-by-hepatitis-b-virus-protein-x-for-viral-persistence
#10
Isha Sengupta, Dipanwita Das, Shivaram Prasad Singh, Runu Chakravarty, Chandrima Das
Promyelocytic leukemia nuclear bodies (PMLNB) are sub-nuclear organelles which are the hub of numerous proteins. DNA/RNA viruses often hijack cellular-factors resident in PML-NBs to promote their proliferation in host cells. Hepatitis B virus (HBV), belonging to Hepadnaviridae family, remains undetected in early infection as it does not induce the innate immune response and is known to be the cause of several hepatic diseases leading to cirrhosis and hepatocellular carcinoma. The association of PML-NB proteins and HBV is being addressed in a number of recent studies...
October 18, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29044868/effects-of-rubiadin-isolated-from-prismatomeris-connata-on-anti-hepatitis-b-virus-activity-in-vitro
#11
Zheng Peng, Gang Fang, Fenghui Peng, Zhiyu Pan, Zhengying Su, Wei Tian, Danrong Li, Huaxin Hou
Prismatomeris connata was a kind of Rubiaceae plant for treatment of hepatitis, hepatic fibrosis and silicosis. Whereas, the effective components of Prismatomeris connata remains unexplored. The aim of this study was to investigate the inhibitory effects and mechanisms of Rubiadin isolated from Prismatomeris connata against HBV using HepG2.2.15 cells. The levels of hepatitis B surface antigen (HBsAg), hepatitis B e antigen (HBeAg), and hepatitis B core antigen (HBcAg) in the supernatants or cytoplasm were examined using by enzyme-linked immunosorbent assay...
October 18, 2017: Phytotherapy Research: PTR
https://www.readbyqxmd.com/read/29029472/up-regulation-of-brain-expressed-x-linked-2-is-critical-for-hepatitis-b-virus-x-protein-induced-hepatocellular-carcinoma-development
#12
Fuqiang Huang, Pei Cai, Yanan Wang, Xian Zhou, Hongyu Chen, Wenjun Liao, Yilei Mao, Xiaojun Zha, Hongbing Zhang, Zhongdong Hu
Hepatocellular carcinoma (HCC) is the third leading cause of cancer-related death worldwide. Chronic hepatitis B virus (HBV) infection is a major cause for HCC. Hepatitis B virus X (HBx), one of four proteins encoded by HBV genome, plays a vital role in the pathogenesis of HBV-induced HCC. However, the molecular mechanisms of HBx-triggered HCC remain largely undetermined. Here we revealed that the expression of Brain-expressed X-linked 2 (BEX2) and Osteopontin (OPN) were elevated in liver tissues of HBV transgenic mice and human HCC specimens...
September 12, 2017: Oncotarget
https://www.readbyqxmd.com/read/29023405/the-aspergillus-flavus-homeobox-gene-hbx1-is-required-for-development-and-aflatoxin-production
#13
Jeffrey W Cary, Pamela Harris-Coward, Leslie Scharfenstein, Brian M Mack, Perng-Kuang Chang, Qijian Wei, Matthew Lebar, Carol Carter-Wientjes, Rajtilak Majumdar, Chandrani Mitra, Sourav Banerjee, Anindya Chanda
Homeobox proteins, a class of well conserved transcription factors, regulate the expression of targeted genes, especially those involved in development. In filamentous fungi, homeobox genes are required for normal conidiogenesis and fruiting body formation. In the present study, we identified eight homeobox (hbx) genes in the aflatoxin-producing ascomycete, Aspergillus flavus, and determined their respective role in growth, conidiation and sclerotial production. Disruption of seven of the eight genes had little to no effect on fungal growth and development...
October 12, 2017: Toxins
https://www.readbyqxmd.com/read/28977838/hepatitis-b-virus-x-protein-induced-upregulation-of-cat-1-stimulates-proliferation-and-inhibits-apoptosis-in-hepatocellular-carcinoma-cells
#14
Rongjuan Dai, Feng Peng, Xinqiang Xiao, Xing Gong, Yongfang Jiang, Min Zhang, Yi Tian, Yun Xu, Jing Ma, Mingming Li, Yue Luo, Guozhong Gong
The HBx protein of hepatitis B virus (HBV) is widely recognized to be a critical oncoprotein contributing to the development of HBV-related hepatocellular carcinoma (HCC). In addition, cationic amino acid transporter 1 (CAT-1) gene is a target of miR-122. In this study, we found that CAT-1 protein levels were higher in HBV-related HCC carcinomatous tissues than in para-cancerous tumor tissues, and that CAT-1 promoted HCC cell growth, proliferation, and metastasis. Moreover, HBx-induced decreases in Gld2 and miR-122 levels that contributed to the upregulation of CAT-1 in HCC...
September 22, 2017: Oncotarget
https://www.readbyqxmd.com/read/28975984/levels-of-hbx-vegf-and-ceacam1-in-hbv-related-hepatocellular-carcinoma-and-their-correlation-with-cancer-prognosis
#15
C-S Mao, H Yin, H-B Ning, Z Peng, K Li, G-Q Ding
OBJECTIVE: Hepatitis B virus X protein (HBx), vascular endothelial growth factor (VEGF) and carcinoembryonic antigen related cell adhesion molecule 1 (CEACAM1), are related to HBV associated hepatocellular carcinoma (HCC). This study recruited HCC patients and employed the SMMC-7721 and L02 liver cell lines, to analyze the expression levels of HBx, VEGF and CEACAM1 in liver cancer and their correlation with the cancer prognosis. PATIENTS AND METHODS: HBV-related HCC patients were recruited from our hospital...
October 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28874700/contribution-of-c1485t-mutation-in-the-hbx-gene-to-human-and-murine-hepatocarcinogenesis
#16
Satoru Hagiwara, Naoshi Nishida, Ah-Mee Park, Yoriaki Komeda, Toshiharu Sakurai, Tomohiro Watanabe, Masatoshi Kudo
Although Hepatitis B virus (HBV) X gene mutations are frequently detected in HBV-related human hepatocellular carcinoma (HCC) patients, causative HBx mutations in the development of HCC have not yet been determined. We herein identified C1485T and C1653T mutations in the HBx gene as independent risk of HCC for HBV through the analysis using serum from chronic hepatitis B patients. We generated transgenic mice expressing wild-type (WT-HBxTg) and mutant (C1485T-HBxTg) HBx to assess the carcinogenic potential of mutated HBx...
September 5, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28846110/hepatitis-b-virus-x-protein-promotes-the-development-of-liver-fibrosis-and-hepatoma-through-downregulation-of-mir-30e-targeting-p4ha2-mrna
#17
G X Feng, J Li, Z Yang, S Q Zhang, Y X Liu, W Y Zhang, L H Ye, X D Zhang
Hepatitis B virus (HBV)-induced liver necrosis takes great part in liver cirrhosis progression. However, less is known about whether hepatitis B virus X protein (HBx) has effect on liver fibrosis. Here, we report that HBV leads to liver fibrosis and hepatocarcinogenesis through miR-30e targeting P4HA2. HBV transgenic mouse was treated by CCl4 to generate a model of liver fibrosis. A crucial enzyme catalyzing collagen formation, prolyl 4-hydroxylase subunit α2 (P4HA2) was evaluated by immunohistochemistry, western blotting or quantitative reverse transcription-PCR analysis...
August 28, 2017: Oncogene
https://www.readbyqxmd.com/read/28822454/-effects-of-up-regulation-of-cathepsin-s-by-hbx-gene-on-hepg2-cell
#18
Z Zhang, P Guo, X Sun, Y Xiao, J Xu, Z S Fang, S Y Wu, H Huang
Objective: To explore the expression of cathepsin S (Cat S) and Hepatitis B virus X protein (HBx) in HBeAg(-) and HBeAg(+ ) hepatocellular carcinoma (HCC), and discuss the effects of Cat S and HBx interaction on HepG2 cell. Methods: Seventy HCC tissue specimens were collected from the surgical resection which were confirmed by pathology in the Fifth Affiliated Hospital of Guangxi Medical University. The tissue samples were separated into two groups: HBeAg(-) group and HBeAg(+ ) group according to the serology of HBeAg...
August 8, 2017: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/28820498/interference-of-apoptosis-by-hepatitis-b-virus
#19
REVIEW
Shaoli Lin, Yan-Jin Zhang
Hepatitis B virus (HBV) causes liver diseases that have been a consistent problem for human health, leading to more than one million deaths every year worldwide. A large proportion of hepatocellular carcinoma (HCC) cases across the world are closely associated with chronic HBV infection. Apoptosis is a programmed cell death and is frequently altered in cancer development. HBV infection interferes with the apoptosis signaling to promote HCC progression and viral proliferation. The HBV-mediated alteration of apoptosis is achieved via interference with cellular signaling pathways and regulation of epigenetics...
August 18, 2017: Viruses
https://www.readbyqxmd.com/read/28810637/binding-of-line-1-rna-to-psf-transcriptionally-promotes-gage6-and-regulates-cell-proliferation-and-tumor-formation-in-vitro
#20
Jiao Lv, Ziyi Zhao
Hepatocellular carcinoma (HCC) has one of the highest mortality rates among numerous types of cancer. It has been demonstrated that in hepatitis B (HBV)-associated HCC, the expression of chimeric fusion transcript HBx-long interspersed nuclear element-1 (LINE-1) initiated by HBV integration is correlated with hepatocarcinogenesis and poor patient survival rates. Furthermore, increased rates of LINE-1 hypomethylation have been detected in HCC tissues compared with adjacent tissues. This suggests that individual LINE-1 RNA (L1 RNA) serves an important role in the processes of hepatocarcinogenesis...
August 2017: Experimental and Therapeutic Medicine
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