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https://www.readbyqxmd.com/read/28328321/ultrafast-and-predictive-mass-spectrometry-based-autotaxin-assays-for-label-free-potency-screening
#1
Tom Bretschneider, Andreas Harald Luippold, Helmut Romig, Daniel Bischoff, Klaus Klinder, Paul Nicklin, Wolfgang Rist
Autotaxin (ATX) is a promising drug target for the treatment of several diseases, such as cancer and fibrosis. ATX hydrolyzes lysophosphatidyl choline (LPC) into bioactive lysophosphatidic acid (LPA). The potency of ATX inhibitors can be readily determined by using fluorescence-based LPC derivatives. While such assays are ultra-high throughput, they are prone to false positives compared to assays based on natural LPC. Here we report the development of ultrafast mass spectrometry-based ATX assays enabling the measurement of data points within 13 s, which is 10 times faster than classic liquid chromatography-mass spectrometry...
April 2017: SLAS Discov
https://www.readbyqxmd.com/read/28315140/electrophysiological-evidence-of-perceived-sexual-attractiveness-for-human-female-bodies-varying-in-waist-to-hip-ratio
#2
Marzia Del Zotto, Alan J Pegna
The dynamics of brain activation reflecting attractiveness in humans are unclear. Among the different features affecting attractiveness of the female body, the waist-to-hip ratio (WHR) is considered to be crucial. To date, however, no event-related potential (ERP) study has addressed the question of its associated pattern of brain activation. We carried out two different experiments: (a) a behavioural study, to judge the level of attractiveness of female realistic models depicting 4 different WHRs (0.6, 0.7, 0...
March 17, 2017: Cognitive, Affective & Behavioral Neuroscience
https://www.readbyqxmd.com/read/28297570/human-lung-spheroids-as-in-vitro-niches-of-lung-progenitor-cells-with-distinctive-paracrine-and-plasticity-properties
#3
Isotta Chimenti, Francesca Pagano, Francesco Angelini, Camilla Siciliano, Giorgio Mangino, Vittorio Picchio, Elena De Falco, Mariangela Peruzzi, Roberto Carnevale, Mohsen Ibrahim, Giuseppe Biondi-Zoccai, Elisa Messina, Giacomo Frati
Basic and translational research on lung biology has discovered multiple progenitor cell types, specialized or facultative, responsible for turnover, renewal, and repair. Isolation of populations of resident lung progenitor cells (LPCs) has been described by multiple protocols, and some have been successfully applied to healthy human lung tissue. We aimed at understanding how different cell culture conditions may affect, in vitro, the phenotype of LPCs to create an ideal niche-like microenvironment. The influence of different substrates (i...
March 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28290263/diagnosis-of-toxoplasmosis-in-pregnancy-evaluation-of-latex-protein-complexes-by-immnunoagglutination
#4
Leandro E Peretti, Verónica D G Gonzalez, Iván S Marcipar, Luis M Gugliotta
The aim of this work was to obtain a reagent based on latex particles for ruling out acute toxoplasmosis in pregnant women by immunoagglutination (IA). Latex-protein complexes (LPC) were previously synthesized coupling the recombinant protein of Toxoplasma gondii P22Ag and the homogenate of the parasite to latex particles with different size, chemical functionality and charge density. LPC were tested in IA assays against a panel of 72 pregnant women serum samples. Results were analysed through receiver operating characteristic curves, determining area under the curve (AUC), sensitivity, specificity positive and negative predictive values (PPV and NPV, respectively)...
March 14, 2017: Parasitology
https://www.readbyqxmd.com/read/28286957/a-combined-clinical-phenotype-and-lipidomic-analysis-reveals-the-impact-of-chronic-kidney-disease-on-lipid-metabolism
#5
Hua Chen, Lin Chen, Dan Liu, Dan-Qian Chen, Nosratola D Vaziri, Xiao-Yong Yu, Li Zhang, Wei Su, Xu Bai, Ying-Yong Zhao
Chronic kidney disease (CKD) results in significant dyslipidemia and profound changes in lipid and lipoprotein metabolism. The associated dyslipidemia, in turn, contributes to progression of CKD and its cardiovascular complications. To gain an in-depth insight into the disorders of lipid metabolism in advanced CKD, we applied UPLC-HDMS-based lipidomics to measure serum lipid metabolites in 180 patients with advanced CKD and 120 age-matched healthy controls. We found significant increases in the levels of total free fatty acids, glycerolipids and glycerophospholipids in patients with CKD...
March 13, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28282695/combined-laser-photocoagulation-and-anti-vegf-injection-treatment-in-radiation-retinopathy
#6
K Hurtikova, H Gerding
Background Radiation retinopathy can lead to a considerable reduction in visual acuity. We report 2 cases of radiation retinopathy in which a therapy with combined intravitreal anti-VEGF injection and laser photocoagulation (LPC) was used. Patient History Two 55-year-old women were referred to our clinic with radiation retinopathy, in the first case unilaterally after radiation due to endocrine orbitopathy (P1), in the second case in both eyes after palliative cerebral radiation for metastatic breast cancer (P2)...
March 10, 2017: Klinische Monatsblätter Für Augenheilkunde
https://www.readbyqxmd.com/read/28262979/mixed-lineage-kinase-3-mediates-the-induction-of-cxcl10-by-a-stat1-dependent-mechanism-during-hepatocyte-lipotoxicity
#7
Kyoko Tomita, Ayano Niibe, Brittany L Freeman, Steven F Bronk, Petra Hirsova, Samar H Ibrahim
BACKGROUND AND AIMS: Saturated free fatty acids (SFA) and their toxic metabolites contribute to hepatocyte lipotoxicity in nonalcoholic steatohepatitis (NASH). We previously reported that hepatocytes, under lipotoxic stress, express the potent macrophage chemotactic ligand C-X-C motif chemokine 10 (CXCL10) and release CXCL10-enriched extracellular vesicles by a mixed lineage kinase (MLK) 3-dependent mechanism. In the current study, we sought to examine the signaling pathway responsible for CXCL10 induction during hepatocyte lipotoxicity...
March 6, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28260516/wounds-difficult-to-heal-an-effective-treatment-strategy
#8
Raffaele Capoano, Rita Businaro, Maria Chiara Tesori, Claudia Donello, Federica Lombardo, Vincenza Rita Lo Vasco, Lorena Capriotti, Mariangela Corsi, Tania Di Raimo, Martina Leopizzi, Bruno Salvati, Serafino Ricci
OBJECTIVE: Treatment of wounds difficult to heal concerns 50% of the elderly population in Italy and is therefore an extreme relevance social burden. The present study shows how the treatment with autologous leuco-platelets reduces the healing time of wounds improving the functional recovery. PATIENTS AND METHODS: One hundred patients with ulcers of the legs were divided in two groups: 1) 50 patients treated with conventional therapies; 2) 50 patients treated with autologous leuco-platelet concentrate (LPC) and HYAFF as a scaffold...
March 1, 2017: Current Vascular Pharmacology
https://www.readbyqxmd.com/read/28255100/improvement-of-myocardial-infarction-risk-prediction-via-inflammation-associated-metabolite-biomarkers
#9
Cavin K Ward-Caviness, Tao Xu, Thor Aspelund, Barbara Thorand, Corinna Montrone, Christa Meisinger, Irmtraud Dunger-Kaltenbach, Astrid Zierer, Zhonghao Yu, Inga R Helgadottir, Tamara B Harris, Lenore J Launer, Andrea Ganna, Lars Lind, Gudny Eiriksdottir, Melanie Waldenberger, Cornelia Prehn, Karsten Suhre, Thomas Illig, Jerzy Adamski, Andreas Ruepp, Wolfgang Koenig, Vilmundur Gudnason, Valur Emilsson, Rui Wang-Sattler, Annette Peters
OBJECTIVE: The comprehensive assaying of low-molecular-weight compounds, for example, metabolomics, provides a unique tool to uncover novel biomarkers and understand pathways underlying myocardial infarction (MI). We used a targeted metabolomics approach to identify biomarkers for MI and evaluate their involvement in the pathogenesis of MI. METHODS AND RESULTS: Using three independent, prospective cohorts (KORA S4, KORA S2 and AGES-REFINE), totalling 2257 participants without a history of MI at baseline, we identified metabolites associated with incident MI (266 cases)...
March 2, 2017: Heart: Official Journal of the British Cardiac Society
https://www.readbyqxmd.com/read/28254008/enzymatic-synthesis-of-lysophosphatidylcholine-with-n-3-polyunsaturated-fatty-acid-from-sn-glycero-3-phosphatidylcholine-in-a-solvent-free-system
#10
Yanjun Liu, Qin Zhang, Yongli Guo, Junyi Liu, Jie Xu, Zhaojie Li, Jingfeng Wang, Yuming Wang, Changhu Xue
The n-3 polyunsaturated fatty acids (PUFA)-rich lysophosphatidylcholine (LPC) was successfully synthesized by Thermomyces lanuginosus lipase (TL IM)-catalyzed esterification of glycerylphosphorylcholine (GPC) and n-3 PUFA-rich fatty acids in a solvent-free system. Effects of reaction temperature, enzyme loading and substrate mole ratio on the yield of LPC and incorporation of n-3 PUFA were evaluated. The acyl-specificities of five enzymes were tested for direct esterification of n-3 PUFA, and Lipozyme TL IM was found to be more effective than others for production of LPC with n-3 PUFA...
July 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28237544/the-language-of-arithmetic-across-the-hemispheres-an-event-related-potential-investigation
#11
Danielle S Dickson, Kara D Federmeier
Arithmetic expressions, like verbal sentences, incrementally lead readers to anticipate potential appropriate completions. Existing work in the language domain has helped us understand how the two hemispheres differently participate in and contribute to the cognitive process of sentence reading, but comparatively little work has been done with mathematical equation processing. In this study, we address this gap by examining the ERP response to provided answers to simple multiplication problems, which varied both in levels of correctness (given an equation context) and in visual field of presentation (joint attention in central presentation, or biased processing to the left or right hemisphere through contralateral visual field presentation)...
February 22, 2017: Brain Research
https://www.readbyqxmd.com/read/28235709/role-of-erk1-2-activation-and-nnos-uncoupling-on-endothelial-dysfunction-induced-by-lysophosphatidylcholine
#12
Gianne P Campos-Mota, Juliana M Navia-Pelaez, Jessica Cristina Araujo-Souza, Nikos Stergiopulos, Luciano S A Capettini
BACKGROUND AND AIMS: Lysophosphatidylcholine (LPC) - a main component of oxidized LDL - is involved in endothelial dysfunction that precedes atherosclerosis, with an increased superoxide anions and a reduced NO production via endothelial NO synthase (eNOS) uncoupling. However, there is no evidence about the mechanisms involved in neuronal NOS (nNOS) uncoupling. Extracellular signal-regulated kinase (ERK) is related to the control of NO production and inflammatory gene transcription activation in atherosclerosis...
March 2017: Atherosclerosis
https://www.readbyqxmd.com/read/28223219/trpm2-contributes-to-lpc-induced-intracellular-ca-2-influx-and-microglial-activation
#13
Heejin Jeong, Yong Ho Kim, Yunsin Lee, Sung Jun Jung, Seog Bae Oh
Microglia are the resident immune cells which become activated in some pathological conditions in central nervous system (CNS). Lysophosphatidylcholine (LPC), an endogenous inflammatory phospholipid, is implicated in immunomodulatory function of glial cells in the CNS. Although several studies uncovered that LPC induces intracellular Ca(2+) influx and morphologic change in microglia, there is still no direct evidence showing change of phosphorylation of mitogen-activated protein kinase (MAPK) p38 (p-p38), a widely used microglia activation marker, by LPC...
April 1, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28216240/gamma-band-neural-synchrony-due-to-autobiographical-fact-violation-in-a-self-referential-question
#14
Jeong Woo Choi, Kwang Su Cha, Ki-Young Jung, Kyung Hwan Kim
Our purpose was to investigate the cortical information processing of autobiographical fact violation (AFV) in self-referential questions. We expected that AFV in a self-referential question would evoke a characteristic pattern of neural synchrony and event-related potentials (ERPs). Averaged ERP and event-related synchronization in the gamma band were analyzed to observe differences between the processing of questions with and without AFV. Significant differences in ERP were found in the posterior area at 290-435 ms (N400) and in the frontal area at 520-800 ms (late positive component, LPC)...
February 16, 2017: Brain Research
https://www.readbyqxmd.com/read/28211537/ambient-ultrafine-particle-ingestion-alters-gut-microbiota-in-association-with-increased-atherogenic-lipid-metabolites
#15
Rongsong Li, Jieping Yang, Arian Saffari, Jonathan Jacobs, Kyung In Baek, Greg Hough, Muriel H Larauche, Jianguo Ma, Nelson Jen, Nabila Moussaoui, Bill Zhou, Hanul Kang, Srinivasa Reddy, Susanne M Henning, Matthew J Campen, Joseph Pisegna, Zhaoping Li, Alan M Fogelman, Constantinos Sioutas, Mohamad Navab, Tzung K Hsiai
Ambient particulate matter (PM) exposure is associated with atherosclerosis and inflammatory bowel disease. Ultrafine particles (UFP, dp < 0.1-0.2 μm) are redox active components of PM. We hypothesized that orally ingested UFP promoted atherogenic lipid metabolites in both the intestine and plasma via altered gut microbiota composition. Low density lipoprotein receptor-null (Ldlr(-/-)) mice on a high-fat diet were orally administered with vehicle control or UFP (40 μg/mouse/day) for 3 days a week...
February 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28195150/molecular-dietary-analysis-of-two-sympatric-felids-in-the-mountains-of-southwest-china-biodiversity-hotspot-and-conservation-implications
#16
Mengyin Xiong, Dajun Wang, Hongliang Bu, Xinning Shao, Dan Zhang, Sheng Li, Rongjiang Wang, Meng Yao
Dietary information is lacking in most of small to mid-sized carnivores due to their elusive predatory behaviour and versatile feeding habits. The leopard cat (LPC; Prionailurus bengalensis) and the Asiatic golden cat (AGC; Catopuma temminckii) are two important yet increasingly endangered carnivore species in the temperate mountain forest ecosystem in Southwest China, a global biodiversity hotspot and a significant reservoir of China's endemic species. We investigated the vertebrate prey of the two sympatric felids using faecal DNA and a next-generation sequencing (NGS)/metabarcoding approach...
February 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28188815/the-impact-of-emotion-intensity-on-recognition-memory-valence-polarity-matters
#17
Xianxin Meng, Ling Zhang, Wenwen Liu, XinSheng Ding, Hong Li, Jiemin Yang, JiaJin Yuan
Although the effects of emotion of different emotional intensity on memory have been investigated, it remain unclear whether the influence of emotional intensity on memory varies depending on the stimulus valence polarity (i.e., positive or negative). To address this, event-related potentials were recorded when subjects performed a continuous old/new discrimination task, for highly negative (HN), mildly negative (MN) and neutral pictures in the negative session; and for highly positive (HP), mildly positive (MP) and neutral pictures in the positive session...
February 7, 2017: International Journal of Psychophysiology
https://www.readbyqxmd.com/read/28187190/widespread-gli-expression-but-limited-canonical-hedgehog-signaling-restricted-to-the-ductular-reaction-in-human-chronic-liver-disease
#18
Candice Alexandra Grzelak, Nicholas David Sigglekow, Janina Elke Eleonore Tirnitz-Parker, Elizabeth Jane Hamson, Alessandra Warren, Bharvi Maneck, Jinbiao Chen, Bramilla Patkunanathan, Jade Boland, Robert Cheng, Nicholas Adam Shackel, Devanshi Seth, David Geoffrey Bowen, Luciano Gastón Martelotto, D Neil Watkins, Geoffrey William McCaughan
Canonical Hedgehog (Hh) signaling in vertebrate cells occurs following Smoothened activation/translocation into the primary cilia (Pc), followed by a GLI transcriptional response. Nonetheless, GLI activation can occur independently of the canonical Hh pathway. Using a murine model of liver injury, we previously identified the importance of canonical Hh signaling within the Pc+ liver progenitor cell (LPC) population and noted that SMO-independent, GLI-mediated signals were important in multiple Pc-ve GLI2+ intrahepatic populations...
2017: PloS One
https://www.readbyqxmd.com/read/28177681/icariin-improves-enos-no-pathway-to-prohibit-the-atherogenesis-of-apolipoprotein-e-null-mice
#19
Hong-Bo Xiao, Guo-Guang Sui, Xiang-Yang Lu
Impaired endothelial nitric oxide synthesis (eNOS) / nitric oxide (NO) pahtway induce the atherogenesis. The present study examined whether icariin improves the eNOS / NO pathway to prohibit the atherogenesis of apolipoprotein E-null (ApoE-/-) mice. In vitro, primary human umbilical vein endothelial cells (HUVECs) were randomly divided into 7 groups: control, vehicle; icariin 10; LPC group; LPC + icariin 1; LPC + icariin 3; LPC + icariin 10. In vivo, 80 mice were separated randomly into four groups (n = 20): control, ApoE-/- , ApoE-/- + icariin 10, and ApoE-/- + icariin 30...
December 22, 2016: Canadian Journal of Physiology and Pharmacology
https://www.readbyqxmd.com/read/28161751/miltefosine-a-novel-internal-standard-approach-to-lysophospholipid-quantitation-using-lc-ms-ms
#20
A Ruth Godfrey, Lewis Jones, Mairead Davies, Rachel Townsend
Understanding and determining levels of lysophospholipids (LPLs) is of increasing interest to the bioanalytical community as they may be targeted for preparative removal as a matrix interference or as a lead substance as a biomarker of disease. Studies monitoring levels of LPLs have used a range of approaches for quantitation whereby those using an internal standard have used either deuterated analogues of the target LPL or alternative LPLs containing an odd number of carbon atoms within its chain, which can be expensive and difficult to distinguish with other LPLs, respectively...
February 4, 2017: Analytical and Bioanalytical Chemistry
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