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https://www.readbyqxmd.com/read/29356218/brain-oxygenation-with-a-non-vasoactive-perfluorocarbon-emulsion-in-a-rat-model-of-traumatic-brain-injury
#1
Rania Abutarboush, Saad H Mullah, Biswajit K Saha, Ashraful Haque, Peter B Walker, Chioma Aligbe, Georgina Pappas, Lam Thuy Vi Tran Ho, Francoise G Arnaud, Charles R Auker, Richard M McCarron, Anke H Scultetus, Paula Moon-Massat
OBJECTIVE: The aim of this study was to assess, in two experiments, the safety and efficacy of the PFC emulsion Oxycyte as an oxygen therapeutic for traumatic brain injury (TBI) to test the hypothesis that early administration of this oxygen-carrying fluid post-TBI would improve brain tissue oxygenation (Pbt O2 ). METHODS: The first experiment assessed effects of Oxycyte on cerebral vasoactivity in healthy, uninjured rats using intravital microscopy. The second experiment investigated the effect of Oxycyte on cerebral Pbt O2 using the phosphorescence quenching method (PQM) in TBI model...
January 22, 2018: Microcirculation: the Official Journal of the Microcirculatory Society, Inc
https://www.readbyqxmd.com/read/29356183/whole-brain-white-matter-histogram-analysis-of-diffusion-tensor-imaging-data-detects-microstructural-damage-in-mild-cognitive-impairment-and-alzheimer-s-disease-patients
#2
Giovanni Giulietti, Mario Torso, Laura Serra, Barbara Spanò, Camillo Marra, Carlo Caltagirone, Mara Cercignani, Marco Bozzali
BACKGROUND: Amnestic mild cognitive impairment (MCI) is a transitional stage between normal aging and Alzheimer's disease (AD). However, the clinical conversion from MCI to AD is unpredictable. Hence, identification of noninvasive biomarkers able to detect early changes induced by dementia is a pressing need. PURPOSE: To explore the added value of histogram analysis applied to measures derived from diffusion tensor imaging (DTI) for detecting brain tissue differences between AD, MCI, and healthy subjects (HS)...
January 21, 2018: Journal of Magnetic Resonance Imaging: JMRI
https://www.readbyqxmd.com/read/29356063/development-and-feasibility-assessment-of-a-3-day-electronic-bladder-diary-as-an-app-for-smart-phone
#3
Laura Mateu Arrom, Lluís Peri Cusi, Agustín Franco de Castro, Luis López-Fando, Miguel Ángel Jiménez Cidre, Antonio Alcaraz Asensio
AIMS: To develop a 3 day bladder diary (BD) as an easy-to-use application for smart-phone (eDM3d). To test its feasibility and acceptance in a reduced number of patients. METHODS: An external agency developed the eDM3d following the structure of the Spanish validated 3 day BD (DM3d©), which includes a frequency-volume chart, the assessment of the grade of urgency, the incontinence events and fluid intake. The eDM3d consisted in a main interface of four buttons ("wake up," "go to bed," "urinate," "drink") which had to be clicked to create an event...
January 22, 2018: Neurourology and Urodynamics
https://www.readbyqxmd.com/read/29355984/pcr-evaluation-of-selected-vector-borne-pathogens-in-dogs-with-pericardial-effusion
#4
M-D Tabar, R Movilla, L Serrano, L Altet, O Francino, X Roura
OBJECTIVES: To investigate evidence for selected vector-borne pathogen infections in dogs with pericardial effusion living in a Mediterranean area in which several canine vector-borne diseases are endemic. MATERIALS AND METHODS: Archived EDTA blood (n=68) and pericardial fluid samples (n=58) from dogs with pericardial effusion (n=68) were included. Dogs without pericardial effusion examined for other reasons were included as controls (n=60). Pericardial effusion was classified as neoplastic in 40 dogs, idiopathic in 23 dogs and of unknown aetiology in 5 dogs...
January 22, 2018: Journal of Small Animal Practice
https://www.readbyqxmd.com/read/29355865/droplet-microfluidic-sans
#5
Marco Adamo, Andreas S Poulos, Carlos G Lopez, Anne Martel, Lionel Porcar, João T Cabral
The coupling of droplet microfluidics and Small Angle Neutron Scattering (SANS) is demonstrated with a range of model systems: isotopic solvent (H2O/D2O) mixtures, surfactant (sodium dodecyl sulfate, SDS) solutions and colloidal (silica) suspensions. Several droplet carrier phases are evaluated and fluorinated oil emerges as a suitable fluid with minimal neutron background scattering (commensurate with air), and excellent interfacial properties. The combined effects of flow dispersion and compositional averaging caused by the neutron beam footprint are evaluated in both continuous and droplet flows and an operational window is established...
January 22, 2018: Soft Matter
https://www.readbyqxmd.com/read/29355815/prophylactic-effect-of-rosmarinic-acid-on-tracheal-responsiveness-white-blood-cell-count-and-oxidative-stress-markers-in-lung-lavage-of-sensitized-rats
#6
Naeima Eftekhar, Ali Moghimi, Mohammad Hossein Boskabady
BACKGROUND: Rosmarinic acid (RA) as an active component of several medicinal plants, has shown anti-inflammatory and anti-oxidant effects. In this study, the effect of RA on tracheal responsiveness (TR), lung inflammatory cells, oxidant biomarkers in sensitized rats were evaluated. METHODS: TR to methacholine and ovalbumin (OVA) as well as total and differential white blood cell (WBC) count and levels of nitrogen dioxide, nitrate, malondialdehyde, thiol, superoxide dismutase, and catalase in bronchoalveolar lavage fluid were measured in control (group C) rats, sensitized animals to OVA and given drinking water alone (group S), S groups receiving drinking water containing three concentrations of RA (0...
January 17, 2018: Pharmacological Reports: PR
https://www.readbyqxmd.com/read/29355743/usefulness-of-sonication-for-diagnosing-infection-in-explanted-orthopaedic-implants
#7
R Erivan, G Villatte, G Eymond, A Mulliez, S Descamps, S Boisgard
BACKGROUND: Orthopaedic implant infection is a rare but serious complication whose optimal treatment requires an accurate microbiological diagnosis. The objective of this study was to determine whether culturing sonicated explants improved sensitivity compared to culturing standard sonicated soft-tissue samples. HYPOTHESIS: Cultures of explant sonication fluid are more sensitive than cultures of soft-tissue sonication fluid in patients with implant infection. METHODS: This single-centre retrospective study included all sonication fluid samples from implants explanted in orthopaedic surgery theatres for any reason...
January 16, 2018: Orthopaedics & Traumatology, Surgery & Research: OTSR
https://www.readbyqxmd.com/read/29355730/extensive-severe-fever-with-thrombocytopenia-syndrome-virus-contamination-in-surrounding-environment-in-patient-rooms
#8
Byung-Han Ryu, Ji Yeun Kim, Taeeun Kim, Min-Chul Kim, Min Jae Kim, Yong-Phil Chong, Sang-Oh Lee, Sang-Ho Choi, Yang Soo Kim, Jun Hee Woo, Sung-Han Kim
OBJECTIVES: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging tick-borne disease in Korea and China. Although there is previous evidence of person-to-person transmission via direct contact with body fluids, the role of environmental contamination by SFTS virus (SFTSV) in healthcare settings has not been established. We therefore investigated the contamination of the healthcare environment by SFTSV. METHODS: We investigated the possible contamination of hospital air and surfaces with SFTSV transmission by collecting air and swabbing environmental surface samples in 2 hospitals treating 6 SFTS patients between March and September 2017...
January 17, 2018: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/29355700/characteristics-of-an-aeromonas-trota-strain-isolated-from-cerebrospinal-fluid
#9
Cibelle B Dallagassa, Monica Surek, Bruno S Vizzotto, Karoline C Prediger, Bárbara Moriel, Suélen Wolf, Vinícius Weiss, Leonardo M Cruz, Flávia E A Assis, Katia S Paludo, Fabiane G M Rego, Sônia M S S Farah, Geraldo Picheth, Emanuel M Souza, Fábio O Pedrosa, Leda S Chubatsu, Cyntia M T Fadel-Picheth
Aeromonas are ubiquitous in aquatic habitats. However some species can cause infections in humans, but rarely meningitis. Here we describe the isolation and characterization of an Aeromonas strain from cerebrospinal fluid of a meningitis patient. The isolate, identified as A. trota by biochemical and molecular methods, was susceptible to ampicillin but resistant to cephalothin and cefazolin. Genome sequencing revealed virulence factor genes such as type VI secretion system, aerolysin and lateral flagella. The isolate exhibited swarming motility, hemolytic activity and adhesion and cytotoxicity on HeLa cells...
January 16, 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29355561/ameliorating-effects-of-d-47-a-newly-developed-compound-on-lipid-metabolism-in-an-animal-model-of-familial-hypercholesterolemia-whhlmi-rabbits
#10
Shohei Tamura, Yui Koike, Hiroaki Takeda, Tomonari Koike, Yoshihiro Izumi, Ryosuke Nagasaka, Tetsuto Tsunoda, Motoo Tori, Kazuo Ogawa, Takeshi Bamba, Masashi Shiomi
Improvements induced in lipid metabolism in the liver by D-47, a newly developed compound, were examined herein. WHHLMI rabbits, an animal model of hypercholesterolemia and coronary atherosclerosis, was fed D-47-supplemented chow for 5 weeks at a dose of 30mg/kg. Lipid concentration were assayed using enzymatic methods. Plasma lipoproteins were fractionated with an ultracentrifuge. mRNA expression was analyzed with real-time PCR. Lipidome analyses of lipoproteins were performed using supercritical fluid chromatography mass spectrometry...
January 16, 2018: European Journal of Pharmacology
https://www.readbyqxmd.com/read/29355487/mesothelioma-with-a-large-prevascular-lymph-node-n1-involvement-or-something-different
#11
Lawek Berzenji, Paul E Van Schil, Annemie Snoeckx, Marjan Hertoghs, Laurens Carp
A 64-year-old man presented with a large amount of right-sided pleural fluid upon imaging, together with calcified pleural plaques and an enlarged nodular structure in the prevascular mediastinum, presumably an enlarged lymph node. Pleural biopsies were obtained during video-assisted thoracoscopic surgery to exclude malignancy. Histopathology showed an epithelial malignant pleural mesothelioma. Induction chemotherapy with cisplatin/pemetrexed was administered followed by an extended pleurectomy/decortication with systematic nodal dissection...
January 18, 2018: Annals of Thoracic Surgery
https://www.readbyqxmd.com/read/29355484/rapid-detection-of-foodborne-pathogen-listeria-monocytogenes-by-strand-exchange-amplification
#12
Meiling Zhang, Xiudan Wang, Lingzhi Han, Shuyan Niu, Chao Shi, Cuiping Ma
A strand exchange amplification (SEA) method to detect foodborne pathogen Listeria monocytogenes was developed. SEA is a novel nucleic acid amplification method that only requires one pair of primers. The specie-specific primers were designed by targeting the 16S rRNA gene and the amplification reaction was performed as short as 60 min at 61 °C. Notably, SEA method could not only detect genomic DNA but also detect RNA by one step without requiring extra reverse transcription. The result could be visualized by naked eyes so that water bath pot would be the only equipment needed...
January 16, 2018: Analytical Biochemistry
https://www.readbyqxmd.com/read/29355276/3d-printed-high-density-reversible-chip-to-chip-microfluidic-interconnects
#13
Hua Gong, Adam T Woolley, Gregory P Nordin
Our latest developments in miniaturizing 3D printed microfluidics [Gong et al., Lab Chip, 2016, 16, 2450; Gong et al., Lab Chip, 2017, 17, 2899] offer the opportunity to fabricate highly integrated chips that measure only a few mm on a side. For such small chips, an interconnection method is needed to provide the necessary world-to-chip reagent and pneumatic connections. In this paper, we introduce simple integrated microgaskets (SIMs) and controlled-compression integrated microgaskets (CCIMs) to connect a small device chip to a larger interface chip that implements world-to-chip connections...
January 22, 2018: Lab on a Chip
https://www.readbyqxmd.com/read/29355167/preoperative-bowel-preparation-complicated-by-lethal-hypermagnesaemia-and-acute-nephropathy
#14
Olumuyiwa A Bamgbade
Hypermagnesaemia is an uncommon but serious disorder. An elderly woman presented with severe cardiovascular collapse, neurologic depression and acute nephropathy, following bowel preparation. Urgent laboratory tests revealed serum magnesium level of 7.5 mmol/L (normal = 0.75-1.05 mmol/L). Prompt resuscitation and treatment of the hypermagnesaemia included intravenous calcium chloride as a physiological antagonist, fluid infusion and frusemide to aid renal excretion of magnesium. There are few case reports of patients who survived hypermagnesaemia levels >7 mmol/L...
October 2017: Nigerian Postgraduate Medical Journal
https://www.readbyqxmd.com/read/29355165/comparison-of-efficacy-of-cell-block-versus-conventional-smear-study-in-exudative-fluids
#15
Sandeep S Matreja, Kamal Malukani, Shirish S Nandedkar, Amit V Varma, Anjali Saxena, Arpita Ajmera
INTRODUCTION: Cytological examination of serous effusions helps in staging, prognostication and management of patients with malignancy. The method has disadvantage of lower sensitivity in differentiating reactive atypical mesothelial cells from malignant cells. AIM: The aim of this study is to compare the cytological features of pleural and peritoneal exudative fluids by conventional smear (CS) method and cell block (CB) method and also to assess the utility of a combined approach for cytodiagnosis of these effusions...
October 2017: Nigerian Postgraduate Medical Journal
https://www.readbyqxmd.com/read/29354988/-effect-of-transcutaneous-electrical-acupoint-stimulation-on-gastric-emptying-in-patients-undergoing-surgery
#16
Yunchang Mo, Anqi Zhang, Bo Zheng, Chengyu Chen, Yelong Ren, Xixi Wang, Haifeng Fu, Lingmin Lin, Junlu Wang
OBJECTIVE: To observe the effects of transcutaneous electrical acupoint stimulation (TEAS) on gastric emptying in patients undergoing selective surgery based on velocity of gastric emptying by ultrasonography. METHODS: A total of 75 patients with selective operation of subarachnoid block at lower limb in the afternoon were randomly assigned to a TEAS group, a sham group and a control group, 25 patients in each one. All the patients were provided with semi-fluid diet at 8 a...
December 12, 2017: Zhongguo Zhen Jiu, Chinese Acupuncture & Moxibustion
https://www.readbyqxmd.com/read/29354929/-comparison-between-five-needles-therapy-and-conventional-acupuncture-for-individual-symptoms-and-signs-of-asthma-of-latent-cold-phlegm-fluid-in-the-lung
#17
Jun Zhang, Suju Shao, Zhong Ren, Peiyu Wang, Jinshuang Hua, Xiaoyong Qin, Qingbo Wang, Suxia Shao
OBJECTIVE: To observe the effect difference between acupuncture of "five needles therapy" and conventional acupuncture for asthma of latent cold phlegm-fluid in the lung. METHODS: Two hundred and ten cases were randomly assigned into an observation group and a control group, 105 cases in each one. Finally 7 cases were dropped out in the observation group; 6 cases in the control group. Feishu (BL 13), Dazhui (GV 14), Fengmen (BL 12) were used in the observation group; conventional acupuncture was used in the control group, and the main acupoints were Feishu (BL 13), Zhongfu (LU 1), Tiantu (CV 22), Danzhong (CV 17), Kongzui (LU 6), Dingchuan (EX-B 1), Fenglong (ST 40), Fengmen (BL 12), Taiyuan (LU 9)...
January 12, 2018: Zhongguo Zhen Jiu, Chinese Acupuncture & Moxibustion
https://www.readbyqxmd.com/read/29354927/-startup-mechanism-of-moxibustion-warming-and-dredging-function
#18
Kaiyu Huang, Shuang Liang, Zheng Sun, Jianbin Zhang
With "moxibustion" and "warm stimulation" as the keywords, the literature on moxibustion mechanism of warming and dredging from June 1st, 1995 to June 1st, 2016 was collected from PubMed, China National Knowledge Infrastructure (CNKI) and Wanfang database. The startup mechanism of moxibustion warming and dredging function was analyzed in terms of moxibustion warming stimulation. The results were found that moxibustion was based on local rising temperature of acupoint. It activated local specific receptors, heat sensitive immune cells, heat shock proteins and so on to start the warming and dredging function and produce various local effects...
September 12, 2017: Zhongguo Zhen Jiu, Chinese Acupuncture & Moxibustion
https://www.readbyqxmd.com/read/29354834/buckling-vs-particle-desorption-in-a-particle-covered-drop-subject-to-compressive-surface-stresses-a-simulation-study
#19
Chuan Gu, Lorenzo Botto
Predicting the behaviour of particle-covered fluid interfaces under compression has implications in several fields. The surface-tension driven adhesion of particles to drops and bubbles is exploited for example to enhance the stability of foams and emulsion and develop new generation materials. When a particle-covered fluid interface is compressed, one can observe either smooth buckling or particle desorption from the interface. The microscopic mechanisms leading to the buckling-to-desorption transition are not fully understood...
January 22, 2018: Soft Matter
https://www.readbyqxmd.com/read/29354645/a-bioactive-hydrogel-and-3d-printed-polycaprolactone-system-for-bone-tissue-engineering
#20
Ivan Hernandez, Alok Kumar, Binata Joddar
In this study, a hybrid system consisting of 3D printed polycaprolactone (PCL) filled with hydrogel was developed as an application for reconstruction of long bone defects, which are innately difficult to repair due to large missing segments of bone. A 3D printed gyroid scaffold of PCL allowed a larger amount of hydrogel to be loaded within the scaffolds as compared to 3D printed mesh and honeycomb scaffolds of similar volumes and strut thicknesses. The hydrogel was a mixture of alginate, gelatin, and nano-hydroxyapatite, infiltrated with human mesenchymal stem cells (hMSC) to enhance the osteoconductivity and biocompatibility of the system...
September 2017: Gels
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