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Adipose derived stem cells

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https://www.readbyqxmd.com/read/28916299/non-canonical-wnt-mediated-neurogenic-differentiation-of-human-bone-marrow-derived-mesenchymal-stem-cells
#1
Sujeong Jang, Hyong-Ho Cho, Jong-Seong Park, Han-Seong Jeong
Bone marrow-derived mesenchymal stem cells (BM-MSCs), which are characterized by multipotency and self-renewal, are responsible for tissue regeneration and repair. We have previously reported in adipose tissue-derived MSCs that only Wnt5a is enhanced at neurogenic differentiation, and the mechanism of differentiation is dependent on the Wnt5a/JNK pathway; however, the role of Wnt/MAPK pathway is yet to be investigated in neurogenic differentiation in BM-MSCs. We compared the transcriptional expression of Wnt in neurogenic induced-hBM-MSCs (NI-hBM-MSCs) with that in primary hBM-MSCs, using RT-PCR, qPCR, and western blotting...
September 12, 2017: Neuroscience Letters
https://www.readbyqxmd.com/read/28916182/erratum-to-overcoming-the-bottleneck-of-platelet-lysate-supply-in-large-scale-clinical-expansion-of-adipose-derived-stem-cells-a-comparison-of-fresh-versus-three-types-of-platelet-lysates-from-outdated-buffy-coat-derived-platelet-concentrates-cytotherapy-2017
#2
Peter V Glovinski, Mikkel Herly, Anders B Mathiasen, Jesper D Svalgaard, Rehannah Borup, Maj-Lis M Talman, Jens J Elberg, Stig-Frederikt Kølle, Krzysztof T Drzewiecki, Anne Fischer-Nielsen
No abstract text is available yet for this article.
October 2017: Cytotherapy
https://www.readbyqxmd.com/read/28914634/cellular-therapy-with-human-autologous-adipose-derived-adult-stem-cells-for-advanced-keratoconus
#3
Pragnya R Donthineni, Bhupesh Bagga, Vivek Singh, Sachin Shukla
No abstract text is available yet for this article.
September 12, 2017: Cornea
https://www.readbyqxmd.com/read/28912813/silicone-substrate-with-collagen-and-carbon-nanotubes-exposed-to-pulsed-current-for-msc-osteodifferentiation
#4
Daniyal Jamal, Roche C de Guzman
Autologous human adipose tissue-derived mesenchymal stem cells (MSCs) have the potential for clinical translation through their induction into osteoblasts for regeneration. Bone healing can be driven by biophysical stimulation using electricity for activating quiescent adult stem cells. It is hypothesized that application of electric current will enhance their osteogenic differentiation, and addition of conductive carbon nanotubes (CNTs) to the cell substrate will provide increased efficiency in current transmission...
2017: International Journal of Biomaterials
https://www.readbyqxmd.com/read/28906099/one-dimension-hydroxyapatite-nanostructures-with-tunable-length-for-efficient-stem-cell-differentiation-regulation
#5
Baojin Ma, Shan Zhang, Feng Liu, Jiazhi Duan, Shicai Wang, Jing Han, Yuanhua Sang, Xiaoqiang Yu, Dong Li, Wei Tang, Shaohua Ge, Hong Liu
It is well-accepted that most osteogenic differentiation processes do need growth factors assistance to improve efficiency. As a material cue, hydroxyapatite (HAp) can promote osteogenic differentiation of stem cells only in a way. Up to now, rare work related to the relationship between HAp nanostructures and stem cells in osteogenic differentiation process without the assistance of growth factors has been reported. In this study, one dimension (1D) HAp nanostructures with tunable length were synthesized by an oleic acid assisted solvothermal method by adjusting the alcohol/water ratio (η)...
September 14, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28905449/lull-pgm-system-a-suitable-technique-to-improve-the-regenerative-potential-of-autologous-fat-grafting
#6
Paolo G Morselli, Federico A Giorgini, Claudia Pazzini, Claudio Muscari
Autologous fat grafting and methods of purification of harvested tissue have become one of the most current themes in regenerative medicine. The aim of this study was to evaluate the in vitro regenerative potential of abdomen lipoaspirates subjected to a combined washing-decantation purifying procedure, the Lull pgm System (Lull). Blood cells and stromal-vascular fraction (SVF) cells contained in the aspirates were investigated and compared with those obtained through more conventional fat-processing methods, i...
September 14, 2017: Wound Repair and Regeneration
https://www.readbyqxmd.com/read/28904532/the-use-of-adipose-derived-stem-cells-in-selected-skin-diseases-vitiligo-alopecia-and-nonhealing-wounds
#7
REVIEW
Agnieszka Owczarczyk-Saczonek, Anna Wociór, Waldemar Placek, Wojciech Maksymowicz, Joanna Wojtkiewicz
The promising results derived from the use of adipose-derived stem cells (ADSCs) in many diseases are a subject of observation in preclinical studies. ADSCs seem to be the ideal cell population for the use in regenerative medicine due to their easy isolation, nonimmunogenic properties, multipotential nature, possibilities for differentiation into various cell lines, and potential for angiogenesis. This article reviews the current data on the use of ADSCs in the treatment of vitiligo, various types of hair loss, and the healing of chronic wounds...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28902341/bilateral-retinal-detachments-after-intravitreal-injection-of-adipose-derived-stem-cells-in-a-patient-with-exudative-macular-degeneration
#8
Steven S Saraf, Matthew A Cunningham, Ajay E Kuriyan, Sarah P Read, Philip J Rosenfeld, Harry W Flynn, Thomas A Albini
A 77-year-old woman with exudative macular degeneration underwent bilateral intravitreal injections of "stem cells" at a clinic in Georgia. One month and 3 months after injection, she developed retinal detachments in the left and right eyes, respectively. Increased awareness within the medical community of such poor outcomes is critical so that clinics offering untested practices that have been shown to be potentially harmful to patients can be identified and brought under U.S. Food and Drug Administration oversight...
September 1, 2017: Ophthalmic Surgery, Lasers & Imaging Retina
https://www.readbyqxmd.com/read/28901689/prevascularization-of-3d-printed-bone-scaffolds-by-bioactive-hydrogels-and-cell-co-culture
#9
Mitchell A Kuss, Shaohua Wu, Ying Wang, Jason B Untrauer, Wenlong Li, Jung Yul Lim, Bin Duan
Vascularization is a fundamental prerequisite for large bone construct development and remains one of the main challenges of bone tissue engineering. Our current study presents the combination of 3D printing technique with a hydrogel-based prevascularization strategy to generate prevascularized bone constructs. Human adipose derived mesenchymal stem cells (ADMSC) and human umbilical vein endothelial cells (HUVEC) were encapsulated within our bioactive hydrogels, and the effects of culture conditions on in vitro vascularization were determined...
September 13, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28901186/recipient-adipose-derived-stem-cells-enhance-recipient-cell-engraftment-and-prolong-allotransplant-survival-in-a-miniature-swine-hind-limb-model
#10
Yur-Ren Kuo, Chien-Chang Chen, Yen-Chou Chen, Ching-Ming Chien
Donor mesenchymal stem cells (MSCs) could prolong vascularized composite allotransplantation (VCA) survival in our previous studies. However, recipient adipose tissue is easier to harvest than donor tissue for preconditioning modulation. Hence, this study investigated the efficacy of recipient autologous adipose-derived stem cells (rADSCs) for VCA survival. The heterotopic hind-limb transplantation from female donor to male recipient was performed in outbred miniature swine. Group I ( n = 6) was untreated controls...
August 2017: Cell Transplantation
https://www.readbyqxmd.com/read/28900631/single-cell-phosphospecific-flow-cytometric-analysis-of-canine-and-murine-adipose-derived-stem-cells
#11
Harumichi Itoh, Shimpei Nishikawa, Tomoya Haraguchi, Yu Arikawa, Masato Hiyama, Toshie Iseri, Yoshiki Itoh, Munekazu Nakaichi, Yasuho Taura, Kenji Tani, Kazuhito Itamoto
This study aimed to demonstrate single-cell phosphospecific flow cytometric analysis of canine and murine adipose-derived stem/stromal cells (ADSCs). ADSCs were obtained from clinically healthy laboratory beagles and C57BL/6 mice. Cell differentiation into adipocytes, osteocytes, and chondrocytes was observed for the cultured canine ADSCs (cADSCs) and murine ADSCs (mADSCs) to determine their multipotency. We also performed single-cell phosphospecific flow cytometric analysis related to cell differentiation and stemness...
2017: Journal of veterinary medicine
https://www.readbyqxmd.com/read/28899664/implant-for-autologous-soft-tissue-reconstruction-using-an-adipose-derived-stem-cell-colonized-alginate-scaffold
#12
Tobias Hirsch, Christine Laemmle, Bjoern Behr, Marcus Lehnhardt, Frank Jacobsen, Dirk Hoefer, Maximilian Kueckelhaus
BACKGROUND: Adipose-derived stem cells represent an interesting option for soft tissue replacement as they are easy to procure and can generate their own blood supply through the production of angiogenic factors. We seeded adipose-derived stem cells on a bioresorbable, biocompatible polymer alginate scaffold to generate autologous soft tissue constructs for repair. MATERIALS AND METHODS: We built and optimized an alginate scaffold and tested its biocompatibility using the MTT assay and its hydration capacity...
August 16, 2017: Journal of Plastic, Reconstructive & Aesthetic Surgery: JPRAS
https://www.readbyqxmd.com/read/28895862/immunomodulatory-effects-of-soluble-factors-secreted-by-feline-adipose-tissue-derived-mesenchymal-stem-cells
#13
Hyung-Kyu Chae, Woo-Jin Song, Jin-Ok Ahn, Qiang Li, Bo-Yeon Lee, Kyeong Kweon, Sang-Chul Park, Hwa-Young Youn
Mesenchymal stem cells (MSCs) have immunomodulatory functions and differentiation capacity, and their clinical use is increasing in veterinary species. Although MSCs have been applied in the treatment in various inflammatory diseases, mechanistic research on feline MSCs is lacking. Accordingly, in this study, we aimed to investigate the immunomodulatory mechanisms of MSCs isolated from feline adipose tissue (fATMSCs). fATMSCs from healthy cats were cultured in an appropriate manner and cocultured with transwell-separated allogeneic feline peripheral blood mononuclear cells (fPBMCs) and RAW264...
September 2017: Veterinary Immunology and Immunopathology
https://www.readbyqxmd.com/read/28894451/inflammatory-conditions-dictate-the-effect-of-mesenchymal-stem-or-stromal-cells-on-b-cell-function
#14
Franka Luk, Laura Carreras-Planella, Sander S Korevaar, Samantha F H de Witte, Francesc E Borràs, Michiel G H Betjes, Carla C Baan, Martin J Hoogduijn, Marcella Franquesa
The immunomodulatory capacity of mesenchymal stem or stromal cells (MSC) makes them a promising tool for treatment of immune disease and organ transplantation. The effects of MSC on B cells are characterized by an abrogation of plasmablast formation and induction of regulatory B cells (Bregs). It is, however, unknown how MSC interact with B cells under inflammatory conditions. In this study, adipose tissue-derived MSC were pretreated with 50 ng/ml IFN-γ for 96 h (MSC-IFN-γ) to simulate inflammatory conditions...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28894288/altered-adipose-derived-stem-cell-characteristics-in-macrodactyly
#15
Xi Yang, Yongkang Jiang, Gang Han, Yuan Shi, Shengbo Zhou, Feng Ni, Bin Wang
Macrodactyly is a congenital disease characterized by aggressive overgrowth of adipose tissue in digits or limbs frequently accompanied with hyperostosis and nerve enlargement; its pathological mechanism is poorly understood. Adipose-derived stem cells (ASCs) have been extensively studied in tissue engineering and regenerative medicine as an ideal alternative substitute for bone marrow-derived mesenchymal stem cells (BM-MSCs), but their pathological role is largely unknown. In this study, ASCs from macrodactyly adipose tissues (Mac-ASCs) were isolated and compared to ASCs derived from the normal abdominal subcutaneous adipose tissue (Sat-ASCs) for cell morphology, surface marker expression, proliferation rate, and tri-lineage differentiation potential...
September 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28891216/the-cd45-fraction-in-murine-adipose-tissue-derived-stromal-cells-harbor-immune-inhibitory-inflammatory-cells
#16
Alessandro Nasti, Yoshio Sakai, Akihiro Seki, Geraldine Belen Buffa, Takuya Komura, Hatsune Mochida, Masatoshi Yamato, Keiko Yoshida, Tuyen T B Ho, Masayuki Takamura, Soichiro Usui, Takashi Wada, Masao Honda, Shuichi Kaneko
Stromal cells in adipose tissue are useful for repair/regenerative therapy as they harbor a substantial number of mesenchymal stem cells; therefore, freshly isolated autologous uncultured adipose tissue-derived stromal cells (u-ADSCs) are useful for regenerative therapy, and obviate the need for mesenchymal stem cells. We evaluated the therapeutic effect of murine u-ADSCs and sorted subsets of u-ADSCs in a concanavalin A (ConA)-induced murine model of hepatitis, as well as their characteristics. We found that 10% to 20% of u-ADSCs expressed the CD45 leukocyte-related antigen...
September 11, 2017: European Journal of Immunology
https://www.readbyqxmd.com/read/28888566/tendon-tissue-engineering-mechanism-and-effects-of-human-tenocyte-coculture-with-adipose-derived-stem-cells
#17
Chao Long, Zhen Wang, Anais Legrand, Arhana Chattopadhyay, James Chang, Paige M Fox
PURPOSE: Adipose-derived stem cells (ASCs) are a potential candidate for cell-based therapy targeting tendon injury; however, their therapeutic benefit relies on their ability to interact with native tenocytes. This study examines the mechanism and effects of coculturing human tenocytes and ASCs. METHODS: Tenocytes (T) were directly cocultured with either ASCs (A) or fibroblasts (F) (negative control) in the following ratios: 50% T/50% A or F; 25% T/75% A or F; and 75% T/25% A or F...
September 6, 2017: Journal of Hand Surgery
https://www.readbyqxmd.com/read/28888046/gap-junctional-intercellular-communication-in-adipose-derived-stromal-stem-cells-is-cell-density-dependent-and-positively-impacts-adipogenic-differentiation
#18
Wiesner Miriam, Berberich Oliver, Hoefner Christiane, Blunk Torsten, Bauer-Kreisel Petra
Adipose-derived stromal/stem cells (ASCs) represent a widely used cell source with multi-lineage differentiation capacity in approaches for tissue engineering and regenerative medicine. Despite the multitude of literature on their differentiation capacity, little is reported about the physiological properties contributing to and controlling the process of lineage differentiation. Direct intercellular communication between adjacent cells via gap junctions has been shown to modulate differentiation processes in other cell types, with connexin 43 (Cx43) being the most abundant isoform of the gap junction-forming connexins...
September 9, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28888019/in-vivo-characterization-of-a-3d-hybrid-scaffold-based-on-pcl-decellularized-aorta-for-tracheal-tissue-engineering
#19
Fariba Ghorbani, Lida Moradi, Mohammad Behgam Shadmehr, Shahin Bonakdar, Atosa Droodinia, Farzaneh Safshekan
INTRODUCTION: As common treatments for long tracheal stenosis are associated with several limitations, tracheal tissue engineering is considered as an alternative treatment. AIM OF STUDY: This study aimed at preparing a hybrid scaffold, based on biologic and synthetic materials for tracheal tissue engineering. MATERIALS AND METHODS: Three electrospun polycaprolactone (PCL) scaffolds, namely E1 (pure PCL), E2 (collagen-coated PCL) and E3 (PCL blended with collagen) were prepared...
December 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28885620/oxidative-stress-induced-s100b-accumulation-converts-myoblasts-into-brown-adipocytes-via-an-nf-%C3%AE%C2%BAb-yy1-mir-133-axis-and-nf-%C3%AE%C2%BAb-yy1-bmp-7-axis
#20
Giulio Morozzi, Sara Beccafico, Roberta Bianchi, Francesca Riuzzi, Ilaria Bellezza, Ileana Giambanco, Cataldo Arcuri, Alba Minelli, Rosario Donato
Muscles of sarcopenic people show hypotrophic myofibers and infiltration with adipose and, at later stages, fibrotic tissue. The origin of infiltrating adipocytes resides in fibro-adipogenic precursors and nonmyogenic mesenchymal progenitor cells, and in satellite cells, the adult stem cells of skeletal muscles. Myoblasts and brown adipocytes share a common Myf5(+) progenitor cell: the cell fate depends on levels of bone morphogenetic protein 7 (BMP-7), a TGF-β family member. S100B, a Ca(2+)-binding protein of the EF-hand type, is expressed at relatively high levels in myoblasts from sarcopenic humans and exerts anti-myogenic effects via NF-κB-dependent inhibition of MyoD, a myogenic transcription factor acting upstream of the essential myogenic factor, myogenin...
September 8, 2017: Cell Death and Differentiation
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