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Asthma AND gene expression

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https://www.readbyqxmd.com/read/28093362/hypoxia-inducible-factor-1%C3%AE-inhibition-modulates-airway-hyperresponsiveness-and-nitric-oxide-levels-in-a-balb-c-mouse-model-of-asthma
#1
Carola Dewitz, Elisa McEachern, Stephanie Shin, Kathryn Akong, Dale G Nagle, David H Broide, Praveen Akuthota, Laura E Crotty Alexander
: Hypoxia inducible factor (HIF)-1α is a master regulator of inflammation and is upregulated in alveolar macrophages and lung parenchyma in asthma. HIF-1α regulates select pathways in allergic inflammation, and thus may drive particular asthma phenotypes. This work examines the role of pharmacologic HIF-1α inhibition in allergic inflammatory airway disease (AIAD) pathogenesis in BALB/c mice, which develop an airway hyperresponsiveness (AHR) asthma phenotype. Systemic treatment with HIF-1α antagonist YC-1 suppressed the increase in HIF-1α expression seen in control AIAD mice...
January 13, 2017: Clinical Immunology: the Official Journal of the Clinical Immunology Society
https://www.readbyqxmd.com/read/28089872/identification-of-airway-mucosal-type-2-inflammation-by-clinical-biomarkers-in-asthma
#2
Philip E Silkoff, Michel Laviolette, Dave Singh, J Mark FitzGerald, Steven Kelsen, Vibeke Backer, Celeste M Porsbjerg, Pierre-Olivier Girodet, Patrick Berger, Joel N Kline, Geoffrey Chupp, Vedrana S Susulic, Elliot S Barnathan, Frédéric Baribaud, Matthew J Loza
BACKGROUND AND OBJECTIVE: The Airways Disease Endotyping for Personalized Therapeutics (ADEPT) study profiled mild, moderate and severe asthma, and non-atopic healthy controls. We explored this dataset to define Type-2 inflammation based on airway-mucosal IL-13-driven gene expression and how this related to clinically-accessible biomarkers. METHODS: IL-13-driven gene expression was evaluated in several human cell lines. We then defined Type-2 status in 25 healthy subjects, 28 mild, 29 moderate, and 26 severe asthmatics, based on airway-mucosal expression of 1) CC-motif chemokine ligand (CCL)-26, (the most differentially expressed gene), 2) periostin, or 3) a multi-gene IL-13 in-vitro signature (IVS)...
January 12, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28079285/a-pathway-based-association-study-reveals-variants-from-wnt-signaling-genes-contributing-to-asthma-susceptibility
#3
A Barreto-Luis, A Corrales, M Acosta-Herrera, C Gonzalez-Colino, J Cumplido, J Martinez-Tadeo, A Carracedo, J Villar, T Carrillo, M Pino-Yanes, C Flores
BACKGROUND: Genetic susceptibility to asthma is currently linked to a handful of genes which have a limited ability to predict the overall disease risk, suggesting the existence of many other genes involved in disease development. Accumulated evidence from association studies in genes related by biological pathways could reveal novel asthma genes. OBJECTIVE: To reveal novel asthma susceptibility genes by means of a pathway-based association study. METHODS: Based on summary data from a previous a genome-wide association study (GWAS) of asthma, we first identified significant biological pathways using a gene-set enrichment analysis...
January 12, 2017: Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28076799/adenylyl-cyclase-type-9-gene-polymorphisms-are-associated-with-asthma-and-allergy-in-brazilian-children
#4
Helena M P Teixeira, Neuza M Alcantara-Neves, Maurício Barreto, Camila A Figueiredo, Ryan S Costa
Asthma is a chronic inflammatory disease of the respiratory tract. This heterogeneous disease is caused by the interaction of interindividual genetic variability and environmental factors. The gene adenylyl cyclase type 9 (ADCY9) encodes a protein called adenylyl cyclase (AC), responsible for producing the second messenger cyclic AMP (cAMP). cAMP is produced by T regulatory cells and is involved in the down-regulation of T effector cells. Failures in cAMP production may be related to an imbalance in the regulatory immune response, leading to immune-mediated diseases, such as allergic disorders...
January 8, 2017: Molecular Immunology
https://www.readbyqxmd.com/read/28075383/soluble-fibre-meal-challenge-reduces-airway-inflammation-and-expression-of-gpr43-and-gpr41-in-asthma
#5
Isabel Halnes, Katherine J Baines, Bronwyn S Berthon, Lesley K MacDonald-Wicks, Peter G Gibson, Lisa G Wood
Short chain fatty acids (SCFAs) are produced following the fermentation of soluble fibre by gut bacteria. In animal models, both dietary fibre and SCFAs have demonstrated anti-inflammatory effects via the activation of free fatty acid receptors, such as G protein-coupled receptor 41 and 43 (GPR41 and GPR43). This pilot study examined the acute effect of a single dose of soluble fibre on airway inflammation-including changes in gene expression of free fatty acid receptors-in asthma. Adults with stable asthma consumed a soluble fibre meal (n = 17) containing 3...
January 10, 2017: Nutrients
https://www.readbyqxmd.com/read/28073664/extract-of-bulbus-fritillaria-cirrhosa-perturbs-spindle-assembly-checkpoint-induces-mitotic-aberrations-and-genomic-instability-in-human-colon-epithelial-cell-line
#6
Xihan Guo, Juan Ni, Jinglun Xue, Xu Wang
BACKGROUND: Bulbus Fritillaria cirrhosa D. Don (BFC) has been used in China as a folk medicine for the treatment of cough and asthma for more than 2000 years. The antitussive and antiasthmatic effects of BFC have been reported before, nevertheless its toxicity and safety have not been documented. This study investigated the possible effects of BFC on spindle assembly checkpoint (SAC), mitotic fidelity and genomic stability in human NCM460 colon epithelial cells. METHODS: Cells were treated with BFC (0, 20, 40, 80 and 160μg/ml) for 24, 48 and 72h and harvested differently according to the biomarkers observed...
January 7, 2017: Experimental and Toxicologic Pathology: Official Journal of the Gesellschaft Für Toxikologische Pathologie
https://www.readbyqxmd.com/read/28068894/strain-differences-in-a-murine-model-of-air-pollutant-induced-nonatopic-asthma-and-rhinitis
#7
Jack R Harkema, Lucas A Hotchkiss, Nicholas A Vetter, Daven N Jackson-Humbles, Ryan P Lewandowski, James G Wagner
Ozone is an irritating gas found in photochemical smog. Epidemiological associations have been made between the onset of asthma and childhood exposures to increasing levels of ambient ozone (i.e., air pollutant-induced nonatopic asthma). Individuals, however, vary in their susceptibility to this outdoor air pollutant, which may be due, in part, to their genetic makeup. The present study was designed to test the hypothesis that there are murine strain-dependent differences in pulmonary and nasal pathologic responses to repeated ozone exposures...
January 2017: Toxicologic Pathology
https://www.readbyqxmd.com/read/28062322/mir-23b-controls-tgf-%C3%AE-1-induced-airway-smooth-muscle-cell-proliferation-via-direct-targeting-of-smad3
#8
Ming Chen, Jianting Shi, Wei Zhang, Linjie Huang, Xiaoling Lin, Zhiqiang Lv, Wei Zhang, Ruiyun Liang, Shanping Jiang
BACKGROUND: MicroRNAs are small yet versatile gene tuners that regulate a variety of cellular processes, including cell growth and proliferation. Here we report that miR-23b inhibited airway smooth muscle cells (ASMCs) proliferation through directly targeting of Smad3. METHODS: We obtained ASMCs by laser capture microdissection of normal and asthmatic mice lung tissues. Mice ASMCs were cultured and induced by TGF-β1. The implication between TGF-β1 and miR-23b in ASMCs were detected by RT-PCR...
January 3, 2017: Pulmonary Pharmacology & Therapeutics
https://www.readbyqxmd.com/read/28057064/the-lncrnas-involved-in-mouse-airway-allergic-inflammation-following-induced-pluripotent-stem-cell-mesenchymal-stem-cell-treatment
#9
Shu-Yue Wang, Xing-Liang Fan, Qiu-Ning Yu, Meng-Xia Deng, Yue-Qi Sun, Wen-Xiang Gao, Cheng-Lin Li, Jian-Bo Shi, Qing-Ling Fu
BACKGROUND: We have previously reported that induced pluripotent stem cell (iPSC)-mesenchymal stem cells (MSCs) alleviated asthma inflammation in mice. Long noncoding RNAs (lncRNAs) were recently reported as being involved in the immune responses. However, whether lncRNAs are associated with iPSC-MSC immunomodulation in allergic inflammation is still unclear. METHODS: Mice were induced into an asthmatic state and received treatment consisting of iPSC-MSCs. Memory T cells isolated from sensitized mice were challenged and co-cultured with iPSC-MSCs in vitro...
January 6, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28056766/lps-induced-modules-of-co-expressed-genes-in-equine-peripheral-blood-mononuclear-cells
#10
Alicja Pacholewska, Eliane Marti, Tosso Leeb, Vidhya Jagannathan, Vincent Gerber
BACKGROUND: Lipopolysaccharide (endotoxin, LPS) is a strong inducer of the innate immune response. It is widespread in our environment, e.g. in house dust and contributes to asthma. Compared to humans, horses are even more sensitive to LPS. However, data on LPS effects on the equine transcriptome are very limited. Using RNA-seq we analysed LPS-induced differences in the gene expression in equine peripheral blood mononuclear cells at the gene and gene-network level in two half-sib families and one group of unrelated horses...
January 5, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28054016/data-set-on-a-study-of-gene-expression-in-peripheral-samples-to-identify-biomarkers-of-severity-of-allergic-and-nonallergic-asthma
#11
Selene Baos, David Calzada, Lucía Cremades, Joaquín Sastre, Joaquín Quiralte, Fernando Florido, Carlos Lahoz, Blanca Cárdaba
This article contains information related to the research article entitled "Biomarkers associated with disease severity in allergic and nonallergic asthma" (S. Baos, D. Calzada, L. Cremades, J. Sastre, J. Quiralte, F. Florido, C. Lahoz, B. Cárdaba, In press). Specifically, the clinical criteria stablished for selecting the study population (n=104 subjects) are described. Moreover, this article describes the criteria for selecting the 94 genes to be analyzed in PBMCs (peripheral blood mononuclear cells), it is provided a description of these genes and a Table with the genes most differentially expressed by clinical phenotypes and, finally it is detailed the experimental methodology followed for studying the protein expression of MSR1 (macrophage scavenger receptor 1), one of the genes evaluated in the research...
February 2017: Data in Brief
https://www.readbyqxmd.com/read/28045928/altered-epithelial-gene-expression-in-peripheral-airways-of-severe-asthma
#12
Akul Singhania, Hitasha Rupani, Nivenka Jayasekera, Simon Lumb, Paul Hales, Neil Gozzard, Donna E Davies, Christopher H Woelk, Peter H Howarth
Management of severe asthma remains a challenge despite treatment with glucocorticosteroid therapy. The majority of studies investigating disease mechanisms in treatment-resistant severe asthma have previously focused on the large central airways, with very few utilizing transcriptomic approaches. The small peripheral airways, which comprise the majority of the airway surface area, remain an unexplored area in severe asthma and were targeted for global epithelial gene expression profiling in this study. Differences between central and peripheral airways were evaluated using transcriptomic analysis (Affymetrix HG U133 plus 2...
2017: PloS One
https://www.readbyqxmd.com/read/28039105/long-acting-%C3%AE-2-agonists-promote-glucocorticoid-mediated-repression-of-nf-%C3%AE%C2%BAb-by-enhancing-expression-of-the-feedback-regulator-tnfaip3
#13
Mohammed Omar Altonsy, Mahmoud M Mostafa, Anthony N Gerber, Robert Newton
Glucocorticoids, or corticosteroids, are effective treatments for many chronic inflammatory diseases and, in mild/moderate asthma, long-acting β2-adrenoceptor agonists (LABAs) enhance the efficacy of inhaled corticosteroids (ICSs) more than increasing the ICS dose. In human bronchial epithelial, BEAS-2B, cells, expression of TNFα-induced protein 3 (TNFAIP3), or A20, a dual-ubiquitin ligase that provides feedback inhibition of NF-κB, was induced by budesonide, an ICS, formoterol, a LABA, and was further enhanced by budesonide/formoterol combination...
December 29, 2016: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/28035981/potential-inhibitory-influence-of-mirna-210-on-regulatory-t-cells-during-epicutaneous-chemical-sensitization
#14
Carrie Mae Long, Ewa Lukomska, Nikki B Marshall, Ajay Nayak, Stacey E Anderson
Toluene diisocyanate (TDI) is a potent low molecular weight chemical sensitizer and a leading cause of chemical-induced occupational asthma. The regulatory potential of microRNAs (miRNAs) has been recognized in a variety of disease states, including allergic disease; however, the roles of miRNAs in chemical sensitization are largely unknown. In a previous work, increased expression of multiple miRNAs during TDI sensitization was observed and several putative mRNA targets identified for these miRNAs were directly related to regulatory T-cell (Treg) differentiation and function including Foxp3 and Runx3...
December 27, 2016: Genes
https://www.readbyqxmd.com/read/28011397/3-methoxy-catalposide-inhibits-inflammatory-effects-in-lipopolysaccharide-stimulated-raw264-7-macrophages
#15
Hyung Won Ryu, Su Ui Lee, Seoghyun Lee, Hyuk-Hwan Song, Tae Hyun Son, Yeah-Un Kim, Heung Joo Yuk, Hyunju Ro, Chong-Kil Lee, Sung-Tae Hong, Sei-Ryang Oh
Pseudolysimachion rotundum var. subintegrum is utilized as a traditional herbal remedy to treat cough, bronchitis, and asthma in Korea, Russia, China, and Europe. Here, we show that 3-methoxy-catalposide, a novel iridoide glycoside isolated from P. rotundum var. subintegrum has the anti-inflammatory activity in lipopolysaccharide (LPS)-stimulated macrophages. The chemical structure of 3-methoxy-catalposide was determined by NMR, optical rotation and HRESIMS. In in vitro experiment, RAW264.7 cells were treated with 3-methoxy-catalposide for 2h before exposure to LPS for different times...
December 20, 2016: Cytokine
https://www.readbyqxmd.com/read/28011367/biomarkers-associated-with-disease-severity-in-allergic-and-nonallergic-asthma
#16
Selene Baos, David Calzada, Lucía Cremades, Joaquín Sastre, Joaquín Quiralte, Fernando Florido, Carlos Lahoz, Blanca Cárdaba
Asthma is a complex, chronic respiratory disease with a wide clinical spectrum. Use of high-throughput technologies has generated a great deal of data that require validation. In this work the objective was to validate molecular biomarkers related to asthmatic disease types in peripheral blood samples and define their relationship with disease severity. With this purpose, ninety-four previously described genes were analyzed by qRT-PCR in 30 healthy control (HC) subjects, 30 patients with nonallergic asthma (NA), 30 with allergic asthma (AA), and 14 patients with allergy (rhinitis) but without asthma (AR)...
December 20, 2016: Molecular Immunology
https://www.readbyqxmd.com/read/28008678/mast-cells-are-permissive-for-rhinovirus-replication-potential-implications-for-asthma-exacerbations
#17
Charlene Akoto, Donna E Davies, Emily J Swindle
BACKGROUND: Human rhinoviruses (HRVs) are a major trigger of asthma exacerbations, with the bronchial epithelium being the major site of HRV infection and replication. Mast cells (MCs) play a key role in asthma where their numbers are increased in the bronchial epithelium with increasing disease severity. OBJECTIVE: In view of the emerging role of MCs in innate immunity and increased localisation to the asthmatic bronchial epithelium, we investigated whether HRV infection of MCs generated innate immune responses which were protective against infection...
December 23, 2016: Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/27996019/intragenic-cftr-duplication-and-5t-12tg-variant-in-a-patient-with-non-classic-cystic-fibrosis
#18
Patricia B S Celestino-Soper, Edward Simpson, Danika Tumbleson Brink, Ty C Lynnes, Stephen Dlouhy, Matteo Vatta, Jana Yeley, Cynthia Brown, Shaochun Bai
Cystic fibrosis (CF) is an autosomal recessive disorder characterized by the accumulation of sticky and heavy mucus that can damage several organs. CF shows variable expressivity in affected individuals, but it typically causes respiratory and digestive complications as well as congenital bilateral absence of the vas deferens in males. Individuals with classic CF usually have variants that produce a defective protein from both alleles of the CFTR gene. Individuals with other variants may present with classic, non-classic, or milder forms of CF due to lower levels of functional CFTR protein...
December 20, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27984699/gene-expression-correlated-to-severe-asthma-characteristics-reveals-heterogeneous-mechanisms-of-severe-disease
#19
Brian D Modena, Eugene R Bleecker, William W Busse, Serpil C Erzurum, Benjamin M Gaston, Nizar N Jarjour, Deborah A Meyers, Jadranka Milosevic, John R Tedrow, Wei Wu, Naftali Kaminski, Sally E Wenzel
RATIONALE: Severe asthma is a heterogeneous disease with multiple molecular mechanisms. Gene expression studies of asthmatic bronchial epithelial cells have provided biological insight and underscored possible mechanistic differences between individuals. OBJECTIVE: Identify networks of genes reflective of underlying biological processes that define severe asthma(SA). METHODS: Airway epithelial cell gene expression from 155 asthmatics and healthy controls(HC) in the Severe Asthma Research Program was analyzed by weighted gene co-expression network analysis (WGCNA) to identify gene networks and profiles associated with severe asthma and its specific characteristics (i...
December 16, 2016: American Journal of Respiratory and Critical Care Medicine
https://www.readbyqxmd.com/read/27978840/validated-and-longitudinally-stable-asthma-phenotypes-based-on-cluster-analysis-of-the-adept-study
#20
Matthew J Loza, Ratko Djukanovic, Kian Fan Chung, Daniel Horowitz, Keying Ma, Patrick Branigan, Elliot S Barnathan, Vedrana S Susulic, Philip E Silkoff, Peter J Sterk, Frédéric Baribaud
BACKGROUND: Asthma is a disease of varying severity and differing disease mechanisms. To date, studies aimed at stratifying asthma into clinically useful phenotypes have produced a number of phenotypes that have yet to be assessed for stability and to be validated in independent cohorts. The aim of this study was to define and validate, for the first time ever, clinically driven asthma phenotypes using two independent, severe asthma cohorts: ADEPT and U-BIOPRED. METHODS: Fuzzy partition-around-medoid clustering was performed on pre-specified data from the ADEPT participants (n = 156) and independently on data from a subset of U-BIOPRED asthma participants (n = 82) for whom the same variables were available...
December 15, 2016: Respiratory Research
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