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Asthma AND gene expression

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https://www.readbyqxmd.com/read/29148977/the-role-of-micrornas-in-chronic-respiratory-disease-recent-insights
#1
Lindsay R Stolzenburg, Ann Harris
Chronic respiratory diseases encompass a group of diverse conditions affecting the airways, which all impair lung function over time. They include cystic fibrosis, idiopathic pulmonary fibrosis, chronic obstructive pulmonary disease, and asthma, which together affect hundreds of millions of people worldwide. MicroRNAs (miRNAs), a class of small non-coding RNAs involved in post-transcriptional gene repression, are now recognized as major regulators in the development and progression of chronic lung disease. Alterations in miRNA abundance occur in lung tissue, inflammatory cells, and freely circulating in blood and are thought to function both as drivers and modifiers of disease...
November 27, 2017: Biological Chemistry
https://www.readbyqxmd.com/read/29146235/macrophages-their-role-activation-and-polarization-in-pulmonary-diseases
#2
REVIEW
Shweta Arora, Kapil Dev, Beamon Agarwal, Pragnya Das, Mansoor Ali Syed
Macrophages, circulating in the blood or concatenated into different organs and tissues constitute the first barrier against any disease. They are foremost controllers of both innate and acquired immunity, healthy tissue homeostasis, vasculogenesis and congenital metabolism. Two hallmarks of macrophages are diversity and plasticity due to which they acquire a wobbling array of phenotypes. These phenotypes are appropriately synchronized responses to a variety of different stimuli from either the tissue microenvironment or - microbes or their products...
November 12, 2017: Immunobiology
https://www.readbyqxmd.com/read/29140131/effects-of-inhaled-corticosteroids-on-the-expression-of-tnf-family-molecules-in-murine-model-of-allergic-asthma
#3
Fei Shi, Yarui Zhang, Chen Qiu, Yi Xiong, Manhui Li, Aijun Shan, Ying Yang, Binbin Li
BACKGROUND: The tumor necrosis factor superfamily member LIGHT (the official gene symbol approved by NCBI Gene Database), an inflammatory factor secreted by T cells after allergen exposure, recently discovered to play crucial roles in asthmatic airway remodeling. However, it is unclear whether LIGHT could be controlled by inhaled corticosteroids, a key component of asthma management. This study was to investigate the effects and potential mechanisms of inhaled budesonide on the expressions of LIGHT and its receptors (LTβR and HVEM) of lung tissues in ovalbumin-sensitized mice...
October 2017: Experimental Lung Research
https://www.readbyqxmd.com/read/29137293/identification-of-novel-genetic-regulations-associated-with-airway-epithelial-homeostasis-using-next-generation-sequencing-data-and-bioinformatics-approaches
#4
Chau-Chyun Sheu, Ming-Ju Tsai, Feng-Wei Chen, Kuo-Feng Chang, Wei-An Chang, Inn-Wen Chong, Po-Lin Kuo, Ya-Ling Hsu
Airway epithelial cells play important roles in airway remodeling. Understanding gene regulations in airway epithelial homeostasis may provide new insights into pathogenesis and treatment of asthma. This study aimed to combine gene expression (GE) microarray, next generation sequencing (NGS), and bioinformatics to explore genetic regulations associated with airway epithelial homeostasis. We analyzed expression profiles of mRNAs (GE microarray) and microRNAs (NGS) in normal and asthmatic bronchial epithelial cells, and identified 9 genes with potential microRNA-mRNA interactions...
October 10, 2017: Oncotarget
https://www.readbyqxmd.com/read/29134693/microglia-from-offspring-of-dams-with-allergic-asthma-exhibit-epigenomic-alterations-in-genes-dysregulated-in-autism
#5
Annie Vogel Ciernia, Milo Careaga, Janine M LaSalle, Paul Ashwood
Dysregulation in immune responses during pregnancy increases the risk of a having a child with an autism spectrum disorder (ASD). Asthma is one of the most common chronic diseases among pregnant women, and symptoms often worsen during pregnancy. We recently developed a mouse model of maternal allergic asthma (MAA) that induces changes in sociability, repetitive, and perseverative behaviors in the offspring. Since epigenetic changes help a static genome adapt to the maternal environment, activation of the immune system may epigenetically alter fetal microglia, the brain's resident immune cells...
November 14, 2017: Glia
https://www.readbyqxmd.com/read/29120588/association-between-serpinb2-gene-expression-by-real-time-pcr-in-respiratory-epithelial-cells-and-atopic-bronchial-asthma-severity
#6
Nissreen E ELBadawy, Randa S Abdel-Latif, Hoda A El-Hady
Atopic bronchial asthma is chronic respiratory diseases of high frequency and high morbidity and mortality especially in patients refractory to the ordinary medical management. This study aimed to investigate the association between Serpin family B Member 2 (SERPINB2) gene expression and bronchial asthma severity. A total of 127 adult patients with asthma were enrolled in this study. Allergic respiratory symptoms were assessed and patients were classified according to Global Initiative for Asthma (GINA) criteria...
January 2017: Egyptian Journal of Immunology
https://www.readbyqxmd.com/read/29115563/ormdl3-may-participate-in-the-pathogenesis-of-bronchial-epithelial%C3%A2-mesenchymal-transition-in-asthmatic-mice-with-airway-remodeling
#7
Qi Cheng, Yunxiao Shang
Asthma is a common chronic respiratory disease in children that is caused by a complex interaction between genetic and environmental factors. Orosomucoid‑like 3 (ORMDL3) is a candidate gene that has been strongly associated with asthma; however, the underlying mechanisms are unknown. ORMDL3 regulates the expression of metalloproteinases and transforming growth factor‑β, and ORMDL3 transgenic mice exhibit increased airway remodeling. Therefore, ORMDL3 may be associated with airway remodeling. The present study attempted to examine the associations between ORMDL3 and the severity of airway remodeling in asthmatic mice, and also to determine whether ORMDL3 induces epithelial‑mesenchymal transition (EMT) in the bronchial epithelium...
November 6, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29115430/expression-purification-and-epitope-analysis-of-pla%C3%A2-a%C3%A2-2-allergen-from-platanus-acerifolia-pollen
#8
De-Wang Wang, Wei-Wei Ni, Yan-Jun Zhou, Wen Huang, Meng-Da Cao, Ling Meng, Ji-Fu Wei
Platanus acerifolia is one of the major sources of outdoor allergens to humans, and can induce allergic asthma, rhinitis, dermatitis and other allergic diseases. Pla a 2 is a polygalacturonase and represents the major allergen identified in P. acerifolia pollen. The aim of the present study was to express and purify Pla a 2, and to predict B and T cell epitopes of Pla a 2. The gene encoding Pla a 2 was cloned into the pET28a vector and subsequently transfected into ArcticExpress™ (DE3) Escherichia coli cells; purified Pla a 2 was analyzed by western blot analysis...
October 26, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29111218/the-notch-pathway-inhibitor-sahm1-abrogates-the-hallmarks-of-allergic-asthma
#9
Alex KleinJan, Irma Tindemans, Jeffrey E Montgomery, Melanie Lukkes, Marjolein J W de Bruijn, Menno van Nimwegen, Ingrid Bergen, Raymond E Moellering, Henk C Hoogsteden, Louis Boon, Derk Amsen, R W Hendriks
BACKGROUND: The Notch signaling pathway has been implicated in the pathogenesis of allergic airway inflammation. Targeting the active Notch transactivation complex by the cell-permeable, hydrocarbon-stapled synthetic peptide SAHM1 was results in genome-wide suppression of Notch-activated genes in leukemic cells and other models. However, efficacy of SAHM1 in allergic asthma models has remained unexplored. OBJECTIVE: We aimed to investigate therapeutic efficacy of SAHM1 in a house dust mite (HDM)-driven asthma model...
October 27, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/29111212/mcpip1-controls-allergic-airway-inflammation-by-suppressing-il-5-producing-th2-cells-through-notch-gata3-pathway
#10
Hui Peng, Huan Ning, Qinghong Wang, Wenbao Lu, Yingzi Chang, Tony T Wang, Jinping Lai, Pappachan E Kolattukudy, Rong Hou, Daniel F Hoft, Mark S Dykewicz, Jianguo Liu
BACKGROUND: Asthmatic and allergic inflammation is mediated by Th2 cytokines (IL-4, IL-5 and IL-13). Though we have learned much about how Th2 cells are differentiated, the Th2 checkpoint mechanisms remain elusive. OBJECTIVES: In this study, we investigate how monocyte chemotactic protein induced protein-1 (MCPIP1, encoded by zc3h12a gene) regulates IL-5-producing Th2 cell differentiation and Th2-mediated inflammation. METHODS: The functions of zc3h12a(-/-) CD4 T cells were evaluated by checking the expression of Th2 cytokines and transcription factors in vivo and in vitro...
October 27, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/29108965/interleukin-1-inhibitory-kappa-b-kinase-epsilon-induced-glycolysis-augment-epithelial-effector-function-and-promote-allergic-airways-disease
#11
Xi Qian, Reem Aboushousha, Cheryl van de Wetering, Shi B Chia, Eyal Amiel, Robert W Schneider, Jos Lj van der Velden, Karolyn G Lahue, Daisy A Hoagland, Dylan T Casey, Nirav Daphtary, Jennifer L Ather, Matthew J Randall, Minara Aliyeva, Kendall E Black, David G Chapman, Lennart K A Lundblad, David H McMillan, Anne E Dixon, Vikas Anathy, Charles G Irvin, Matthew E Poynter, Emiel F M Wouters, Pamela M Vacek, Monique Henket, Florence Schleich, Renaud Louis, Albert van der Vliet, Yvonne M W Janssen-Heininger
BACKGROUND: Emerging studies suggest that enhanced glycolysis accompanies inflammatory responses. Virtually nothing is known about the relevance of glycolysis in allergic asthma. OBJECTIVES: Here we sought to determine if glycolysis is altered in allergic asthma and to address its importance in the pathogenesis of allergic asthma. METHODS: We examined alterations in glycolysis in sputum samples from asthmatics and primary human nasal cells, and used murine models of allergic asthma as well as primary mouse tracheal epithelial cells to evaluate the relevance of glycolysis...
November 3, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/29106997/interferon-response-to-rsv-by-bronchial-epithelium-from-children-with-asthma-is-inversely-correlated-with-pulmonary-function
#12
M C Altman, S R Reeves, A R Parker, E Whalen, K M S Misura, K A Barrow, R G James, T S Hallstrand, S F Ziegler, J S Debley
BACKGROUND: Respiratory viral infection in early childhood, including that from respiratory syncytial virus (RSV), has been previously associated with the development of asthma. OBJECTIVE: We aimed to determine whether ex vivo RSV infection of bronchial epithelial cells (BECs) from children with asthma would induce specific gene expression patterns, and whether such patterns associate with lung function among BEC donors. METHODS: Primary BECs from carefully characterized children with asthma (n=18) and matched healthy children without asthma (n=8) were differentiated at an air-liquid interface (ALI) for 21 days...
October 26, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/29104649/small-interfering-rna-directed-against-microrna-155-delivered-by-a-lentiviral-vector-attenuates-asthmatic-features-in-a-mouse-model-of-allergic-asthma
#13
Huilong Chen, Xiangqin Xu, Sheng Cheng, Yuzhu Xu, Qi Xuefei, Yong Cao, Jungang Xie, Cong-Yi Wang, Yongjian Xu, Weining Xiong
Asthma is a chronic T helper type 2 (Th2) cell-mediated inflammatory disease characterized by airway hyperresponsiveness (AHR) and airway inflammation. Although the majority of patients with asthma can achieve a good level of control with existing treatments, asthma runs a chronic course and the effectiveness of current treatment is not satisfactory for certain patients. MicroRNAs (miRNAs) are short noncoding RNAs that suppress gene expression at the post-transcriptional level; their role in regulating allergic inflammation remains largely unknown...
November 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29103994/sputum-cell-interleukin-1-receptor-expression-level-is-a-marker-of-airway-neutrophilia-and-airflow-obstruction-in-asthma
#14
Michael D Evans, Stephane Esnault, Loren C Denlinger, Nizar N Jarjour
BACKGROUND: Various clinical, biologic or physiologic markers of asthma have been used to identify patient clusters and potential targets for therapy. However, these identifiers frequently overlap among the different asthma groups. For instance, both eosinophils and neutrophils are often increased in the airway of asthma patients despite their typical association with type-2 and type-17 immune response, respectively. OBJECTIVES: To determine whether inflammatory gene expressions are related to patterns of airway inflammation and lung function, and identify molecular markers for neutrophilic asthma...
November 2, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/29083406/shared-genetic-origin-of-asthma-hay-fever-and-eczema-elucidates-allergic-disease-biology
#15
Manuel A Ferreira, Judith M Vonk, Hansjörg Baurecht, Ingo Marenholz, Chao Tian, Joshua D Hoffman, Quinta Helmer, Annika Tillander, Vilhelmina Ullemar, Jenny van Dongen, Yi Lu, Franz Rüschendorf, Jorge Esparza-Gordillo, Chris W Medway, Edward Mountjoy, Kimberley Burrows, Oliver Hummel, Sarah Grosche, Ben M Brumpton, John S Witte, Jouke-Jan Hottenga, Gonneke Willemsen, Jie Zheng, Elke Rodríguez, Melanie Hotze, Andre Franke, Joana A Revez, Jonathan Beesley, Melanie C Matheson, Shyamali C Dharmage, Lisa M Bain, Lars G Fritsche, Maiken E Gabrielsen, Brunilda Balliu, Jonas B Nielsen, Wei Zhou, Kristian Hveem, Arnulf Langhammer, Oddgeir L Holmen, Mari Løset, Gonçalo R Abecasis, Cristen J Willer, Andreas Arnold, Georg Homuth, Carsten O Schmidt, Philip J Thompson, Nicholas G Martin, David L Duffy, Natalija Novak, Holger Schulz, Stefan Karrasch, Christian Gieger, Konstantin Strauch, Ronald B Melles, David A Hinds, Norbert Hübner, Stephan Weidinger, Patrik K E Magnusson, Rick Jansen, Eric Jorgenson, Young-Ae Lee, Dorret I Boomsma, Catarina Almqvist, Robert Karlsson, Gerard H Koppelman, Lavinia Paternoster
Asthma, hay fever (or allergic rhinitis) and eczema (or atopic dermatitis) often coexist in the same individuals, partly because of a shared genetic origin. To identify shared risk variants, we performed a genome-wide association study (GWAS; n = 360,838) of a broad allergic disease phenotype that considers the presence of any one of these three diseases. We identified 136 independent risk variants (P < 3 × 10(-8)), including 73 not previously reported, which implicate 132 nearby genes in allergic disease pathophysiology...
October 30, 2017: Nature Genetics
https://www.readbyqxmd.com/read/29071794/posttranscriptional-control-of-airway-inflammation
#16
REVIEW
Wendy Ezegbunam, Robert Foronjy
Acute inflammation in the lungs is a vital protective response, efficiently and swiftly eliminating inciters of tissue injury. However, in respiratory diseases characterized by chronic inflammation, such as chronic obstructive pulmonary disease and asthma, enhanced expression of inflammatory mediators leads to tissue damage and impaired lung function. Although transcription is an essential first step in the induction of proinflammatory genes, tight regulation of inflammation requires more rapid, flexible responses...
October 26, 2017: Wiley Interdisciplinary Reviews. RNA
https://www.readbyqxmd.com/read/29067999/myd88-dependent-dendritic-and-epithelial-cell-crosstalk-orchestrates-immune-responses-to-allergens
#17
S Y Thomas, G S Whitehead, M Takaku, J M Ward, X Xu, K Nakano, M R Lyons-Cohen, H Nakano, K M Gowdy, P A Wade, D N Cook
Sensitization to inhaled allergens is dependent on activation of conventional dendritic cells (cDCs) and on the adaptor molecule, MyD88. However, many cell types in the lung express Myd88, and it is unclear how signaling in these different cell types reprograms cDCs and leads to allergic inflammation of the airway. By combining ATAC-seq with RNA profiling, we found that MyD88 signaling in cDCs maintained open chromatin at select loci even at steady state, allowing genes to be rapidly induced during allergic sensitization...
October 25, 2017: Mucosal Immunology
https://www.readbyqxmd.com/read/29066321/macrophage-polarization-and-allergic-asthma
#18
REVIEW
Arjun Saradna, Danh C Do, Shruthi Kumar, Qing-Ling Fu, Peisong Gao
Allergic asthma is associated with airway inflammation and airway hyperresponsiveness. Macrophage polarization has been shown to have a profound impact on asthma pathogenesis. On exposure to local microenvironments, recruited macrophages can be polarized into either classically activated (or M1) or alternatively activated (or M2) phenotypes. Macrophage polarization has been heavily associated with development of asthma. The process of regulation of macrophage polarization involves an intricate interplay between various cytokines, chemokines, transcriptional factors, and immune-regulatory cells...
October 7, 2017: Translational Research: the Journal of Laboratory and Clinical Medicine
https://www.readbyqxmd.com/read/29064281/interferon-stimulated-gene-expression-type-2-inflammation-and-endoplasmic-reticulum-stress-in-asthma
#19
Nirav R Bhakta, Stephanie A Christenson, Srilaxmi Nerella, Owen D Solberg, Christine P Nguyen, David F Choy, Kyle L Jung, Suresh Garudadri, Luke R Bonser, Joshua L Pollack, Lorna T Zlock, David J Erle, Charles Langelier, Joseph L Derisi, Joseph R Arron, John V Fahy, Prescott G Woodruff
RATIONALE: Quantification of type-2 inflammation provided a molecular basis for heterogeneity in asthma. Non-type-2 pathways that contribute to asthma pathogenesis are not well understood. OBJECTIVES: To identify dysregulated pathways beyond type-2 inflammation. METHODS: We applied RNA sequencing to airway epithelial brushings obtained from stable mild asthmatics not on corticosteroids (n=19) and healthy controls (n=16). Sequencing reads were mapped to human and viral genomes...
October 24, 2017: American Journal of Respiratory and Critical Care Medicine
https://www.readbyqxmd.com/read/29059618/development-of-a-robust-reporter-gene-based-assay-for-the-bioactivity-determination-of-il-5-targeted-therapeutic-antibodies
#20
Zhihao Fu, Chuanfei Yu, Lan Wang, Kai Gao, Gangling Xu, Wenbo Wang, Junxia Cao, Junzhi Wang
Eosinophilic asthma is characterized by the eosinophilic inflammation with the allergen independent activation of Th2 lymphocytes. Since IL-5 plays an important role in the maturation, survival and migration of eosinophils, hence the pathogenesis of eosinophilic asthma, biotherapeutics targeting IL-5/IL-5Rα have been developed and/or marketed, including Mepolizumab, Reslizumab, and Benralizumab. Accurate determination of bioactivity is crucial for the safety and efficacy of therapeutic antibodies. The current mode of action (MOA) based method used in the quality control and stability tests for anti-IL-5 mAbs is anti-proliferation assay, which is tedious with long duration and high variation...
September 28, 2017: Journal of Pharmaceutical and Biomedical Analysis
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