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Asthma AND gene expression

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https://www.readbyqxmd.com/read/29778524/posttranscriptional-regulation-of-interleukin-33-expression-by-microrna-200-in-bronchial-asthma
#1
Xin Tang, Feng Wu, Jinshuo Fan, Yang Jin, Jianjun Wang, Guanghai Yang
The importance of understanding how interleukin-33 (IL-33) is regulated (particularly by miRs) is critical in IL-33 biology, and evidence of this in asthma pathology is limited. MicroRNA profiling of cells isolated from bronchoalveolar lavage of 14 asthmatic patients and 11 healthy controls revealed miR-200b and miR-200c were significantly reduced in asthmatic patients compared with healthy controls. The reduction was validated in two independent models of allergen-induced allergic airway inflammation and further demonstrated to be inversely correlated with asthma severity, as well as increased IL-33 production in asthmatic patients...
April 25, 2018: Molecular Therapy: the Journal of the American Society of Gene Therapy
https://www.readbyqxmd.com/read/29777594/il-3-producing-basophils-are-required-to-exacerbate-airway-hyper-responsiveness-in-a-murine-inflammatory-model
#2
Rachel Rignault-Bricard, François Machavoine, Salah Mecheri, Olivier Hermine, Elke Schneider, Michel Dy, Maria Leite-de-Moraes
BACKGROUND: Basophils are commonly associated with allergic responses because of their ability to produce large amounts of pro-Th2 cytokines and histamine. However, the mechanisms through which bone marrow-resident basophils (BMRB) become fully competent cytokine and histamine producers in response to IgE crosslinking are poorly understood. Here, we sought to determine the role of IL-3 in promoting pro-Th2 basophils. METHODS: BMRB and basophils exposed to IL-3 in vitro and in vivo were evaluated for their production of Th2 cytokines and histamine in response to FcεRI crosslinking on both protein and gene expression levels...
May 19, 2018: Allergy
https://www.readbyqxmd.com/read/29775004/-detection-of-differentially-expressed-gene-of-allergic-rhinitis-based-on-rt%C3%A2-profiler-pcr-array
#3
Y Peng, X Q Li, Q H Qiu
Objective: The aim of this study is to detect differentially expressed gene of allergic rhinitis (AR) based on RT² profiler PCR array. Method: The real-time PCR array containing 84 genes were used to compare inferior turbinate samples obtained from AR patients (n=8) and healthy individuals (n=8). Result: The results showed that 67 genes were down regulated and 17 genes were up regulated in AR inferior turbinate after analysis of 84 genes related to allergy and asthma. STAT6 and BCL6 were recognized as differentially expressed genes...
June 5, 2017: Journal of Clinical Otorhinolaryngology, Head, and Neck Surgery
https://www.readbyqxmd.com/read/29769273/genome-wide-posttranscriptional-dysregulation-by-micrornas-in-human-asthma-as-revealed-by-frac-seq
#4
Rocio T Martinez-Nunez, Hitasha Rupani, Manuela Platé, Mahesan Niranjan, Rachel C Chambers, Peter H Howarth, Tilman Sanchez-Elsner
MicroRNAs are small noncoding RNAs that inhibit gene expression posttranscriptionally, implicated in virtually all biological processes. Although the effect of individual microRNAs is generally studied, the genome-wide role of multiple microRNAs is less investigated. We assessed paired genome-wide expression of microRNAs with total (cytoplasmic) and translational (polyribosome-bound) mRNA levels employing subcellular fractionation and RNA sequencing (Frac-seq) in human primary bronchoepithelium from healthy controls and severe asthmatics...
May 16, 2018: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/29765562/a-myriad-of-roles-of-mir-25-in-health-and-disease
#5
REVIEW
Márta Sárközy, Zsuzsanna Kahán, Tamás Csont
Small non-coding RNAs including microRNAs (miRNAs) have been recently recognized as important regulators of gene expression. MicroRNAs play myriads of roles in physiological processes as well as in the pathogenesis of a number of diseases by translational repression or mRNA destabilization of numerous target genes. The miR-106b-25 cluster is highly conserved in vertebrates and consists of three members including miR-106b, miR-93 and miR-25. MiR-106b and miR-93 share the same seed sequences; however, miR-25 has only a similar seed sequence resulting in different predicted target mRNAs...
April 20, 2018: Oncotarget
https://www.readbyqxmd.com/read/29758111/genetic-profiles-of-transcriptomic-clusters-of-childhood-asthma-determine-specific-severe-subtype
#6
Y-L Yeh, M-W Su, B-L Chiang, Y-H Yang, C-H Tsai, Y L Lee
BACKGROUND: Previous studies have defined transcriptomic subtypes of adult asthma using samples of induced sputum and bronchial epithelium; however, those procedures are not readily applicable in the clinic, especially for childhood asthma. OBJECTIVE: We aim to dissect the transcriptomic clusters of childhood asthma using highly variably expressed genes of peripheral blood mononuclear cells (PBMC) among patients. METHODS: Gene expression of PBMC from 133 asthmatic children and 11 healthy controls was measured with Illumina microarrays...
May 14, 2018: Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/29758105/transcriptional-profiling-of-eosinophil-subsets-in-interleukin-5-transgenic-mice
#7
Kirsten A Fairfax, Jessica E Bolden, Aaron J Robinson, Erin C Lucas, Tracey M Baldwin, Kerry A Ramsay, Rebecca Cole, Douglas J Hilton, Carolyn A de Graaf
Eosinophils are important in fighting parasitic infections and are implicated in the pathogenesis of asthma and allergy. IL-5 is a critical regulator of eosinophil development, controlling proliferation, differentiation, and maturation of the lineage. Mice that constitutively express IL-5 have in excess of 10-fold more eosinophils in the hematopoietic organs than their wild type (WT) counterparts. We have identified that much of this expansion is in a population of Siglec-F high eosinophils, which are rare in WT mice...
May 14, 2018: Journal of Leukocyte Biology
https://www.readbyqxmd.com/read/29757584/effects-of-nerve-growth-factor-shrna-inhibition-on-asthma-phenotypes-in-a-mouse-model-of-asthma
#8
Jinhong Wu, Bing Chen, Wenxian Li, Yanan Xiao
Nerve growth factor (NGF) plays an important role in airway hyper-responsiveness (AHR). In this study, we aimed at investigating the effect of NGF inhibition on AHR and other asthma phenotypes in a mouse model of asthma. 12 mice in each group were injected with lentiviral vectors expressing non-targeting shRNA (sham shRNA), targeting NGF (shRNA-1 and shRNA-2), or normal saline for control before the asthma models were established. Peak inspiratory pressure (PIP), NGF levels in bronchoalveolar lavage fluid (BALF), and bronchoconstriction in response to acetylcholine (ACh) were measured...
April 2018: Iranian Journal of Allergy, Asthma, and Immunology
https://www.readbyqxmd.com/read/29748238/mir-302e-attenuates-allergic-inflammation-in-vitro-model-by-targeting-rela
#9
Lifeng Xiao, Li Jiang, Qi Hu, Yuru Li
Allergic inflammation is the foundation of allergic rhinitis and asthma. Although microRNAs are implicated in the pathogenesis of various diseases, information regarding the functional role of microRNAs in allergic diseases is limited. Herein, we reported that microRNA-302e (miR-302e) serves as an important regulator of allergic inflammation in human mast cell line, HMC-1 cells. Our results showed that miR-302e is the dominant member of miR-302 family expressed in HMC-1 cells. Moreover, the expression of miR-302e was significantly decreased in response to phorbol 12-myristate 13-acetate (PMA) and calcium ionophore A23187 or ovalbumin (OVA) stimulation...
May 10, 2018: Bioscience Reports
https://www.readbyqxmd.com/read/29747740/auraptene-regulates-th-1-th-2-t-reg-balances-nf-%C3%AE%C2%BAb-nuclear-localization-and-nitric-oxide-production-in-normal-and-th-2-provoked-situations-in-human-isolated-lymphocytes
#10
Vahid Reza Askari, Vafa Baradaran Rahimi, Seyed Abdolrahim Rezaee, Mohammad Hossein Boskabady
BACKGROUND: Auraptene as member of dietary coumarins, is found in citrus fruits. Former studies have demonstrated its anti-inflammatory and anti-cancer activity. PURPOSE: The mechanism of action and immune-modulatory property of this compound on human lymphocytes are greatly unknown. STUDY DESIGN/METHODS: The effect of three concentrations (10, 30 and 90 µM) of auraptene or dexamethasone (0.1 mM) were evaluated on percentage of cell proliferation and nitric oxide (NO) production as well as secretion and gene expression of cytokines, and NF-κB level in PHA-stimulated and non-stimulated lymphocytes...
April 1, 2018: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/29746147/female-sex-and-gender-in-lung-sleep-health-and-disease-increased-understanding-of-basic-biological-pathophysiological-and-behavioral-mechanisms-leading-to-better-health-for-female-patients-with-lung-disease
#11
MeiLan K Han, Emilio Arteaga-Solis, John Blenis, Ghada Bourjeily, Deborah J Clegg, Dawn DeMeo, Jeanne Duffy, Ben Gaston, Nicola M Heller, Anna Hemnes, Elizabeth Petri Henske, Raksha Jain, Tim Lahm, Lisa H Lancaster, Joyce Lee, Marianne J Legato, Sherry McKee, Reena Mehra, Alison Morris, Y S Prakash, Martin R Stampfli, Rashmi Gopal-Srivastava, Aaron D Laposky, Antonello Punturieri, Lora Reineck, Xenia Tigno, Janine Clayton
Female sex/gender is an under-characterized variable in studies related to lung development and disease. Notwithstanding, many aspects of lung and sleep biology and pathobiology are impacted by female sex and female reproductive transitions. These may manifest as differential gene expression or peculiar organ development. Some conditions are more prevalent in women, such as asthma and insomnia, or in the case of LAM, are seen almost exclusively in women. In other diseases, presentation differs such as the higher frequency of exacerbations experienced by women with COPD or greater cardiac morbidity among women with sleep disordered breathing...
May 10, 2018: American Journal of Respiratory and Critical Care Medicine
https://www.readbyqxmd.com/read/29743346/pgd2-dp2-receptor-activation-promotes-severe-viral-bronchiolitis-by-suppressing-ifn-%C3%AE-production
#12
Rhiannon B Werder, Jason P Lynch, Jennifer C Simpson, Vivian Zhang, Nick H Hodge, Matthew Poh, Elizabeth Forbes-Blom, Christina Kulis, Mark L Smythe, John W Upham, Kirsten Spann, Mark L Everard, Simon Phipps
Prostaglandin D2 (PGD2) signals through PGD2 receptor 2 (DP2, also known as CRTH2) on type 2 effector cells to promote asthma pathogenesis; however, little is known about its role during respiratory syncytial virus (RSV) bronchiolitis, a major risk factor for asthma development. We show that RSV infection up-regulated hematopoietic prostaglandin D synthase expression and increased PGD2 release by cultured human primary airway epithelial cells (AECs). Moreover, PGD2 production was elevated in nasopharyngeal samples from young infants hospitalized with RSV bronchiolitis compared to healthy controls...
May 9, 2018: Science Translational Medicine
https://www.readbyqxmd.com/read/29729044/nasal-mucosa-and-blood-cell-transcriptome-profiles-do-not-reflect-respiratory-symptoms-associated-with-moisture-damage
#13
Joseph Ndika, Hille Suojalehto, Martin Täubel, Maili Lehto, Kirsi Karvala, Paula Pallasaho, Jukka Sund, Petri Auvinen, Kati Järvi, Juha Pekkanen, Pia Kinaret, Dario Greco, Anne Hyvärinen, Harri Alenius
Upper and lower respiratory symptoms and asthma are adverse health effects associated with moisture-damaged buildings. Quantitative measures to detect adverse health effects related to exposure to dampness and mold are needed. Here, we investigate differences in gene expression between occupants of moisture-damaged and reference buildings. Moisture-damaged (N=11) and control (N=5) buildings were evaluated for dampness and mold by trained inspectors. The transcriptomics cohort consisted of nasal brushings and peripheral blood mononuclear cells (PBMCs) from 86 teachers, with/without self-perceived respiratory symptoms...
May 4, 2018: Indoor Air
https://www.readbyqxmd.com/read/29726087/asthma-and-allergic-rhinitis-associate-with-the-rs2229542-variant-that-induces-a-p-lys90glu-mutation-and-compromises-akr1b1-protein-levels
#14
Elena García-Martín, Francisco J Sánchez-Gómez, Gemma Amo, Jesús García Menaya, Concepción Cordobés, Pedro Ayuso, M Carmen Plaza Serón, Miguel Blanca, Paloma Campo, Gara Esguevillas, María A Pajares, José A G Agúndez, Dolores Pérez-Sala
Asthma and rhinitis are two of the main clinical manifestations of allergy, in which increased reactive oxygen or electrophilic species can play a pathogenic role. Aldose reductase (AKR1B1) is involved in aldehyde detoxification and redox balance. Recent evidence from animal models points to a role of AKR1B1 in asthma and rhinitis, but its involvement in human allergy has not been addressed. Here, the putative association of allergic rhinitis and asthma with AKR1B1 variants has been explored by analysis of single-strand variants on the AKR1B1 gene sequence in 526 healthy subjects and 515 patients with allergic rhinitis, 366 of whom also had asthma...
May 3, 2018: Human Mutation
https://www.readbyqxmd.com/read/29724254/radical-containing-combustion-derived-particulate-matter-enhance-pulmonary-th17-inflammation-via-the-aryl-hydrocarbon-receptor
#15
Sridhar Jaligama, Vivek S Patel, Pingli Wang, Asmaa Sallam, Jeffrey Harding, Matthew Kelley, Skylar R Mancuso, Tammy R Dugas, Stephania A Cormier
BACKGROUND: Pollutant particles containing environmentally persistent free radicals (EPFRs) are formed during many combustion processes (e.g. thermal remediation of hazardous wastes, diesel/gasoline combustion, wood smoke, cigarette smoke, etc.). Our previous studies demonstrated that acute exposure to EPFRs results in dendritic cell maturation and Th17-biased pulmonary immune responses. Further, in a mouse model of asthma, these responses were enhanced suggesting exposure to EPFRs as a risk factor for the development and/or exacerbation of asthma...
May 3, 2018: Particle and Fibre Toxicology
https://www.readbyqxmd.com/read/29721720/comparison-of-paired-human-nasal-and-bronchial-airway-epithelial-cell-responses-to-rhinovirus-infection-and-il-13-treatment
#16
Nicole Roberts, Reem Al Mubarak, David Francisco, Monica Kraft, Hong Wei Chu
BACKGROUND: Because of its advantage as a minimally invasive procedure, nasal brushings have been increasingly used and proposed as a valuable approach to study lower airway diseases in lieu of bronchial epithelial cells. However, there is limited or conflicting evidence pertaining to whether nasal samples can be surrogates to bronchial samples. The goal of the present study is to test whether nasal epithelial cells have similar antiviral and inflammatory responses to IL-13 treatment and rhinovirus infection, a condition mimicking virally induced asthma exacerbation...
May 2, 2018: Clinical and Translational Medicine
https://www.readbyqxmd.com/read/29721367/tumor-derived-thymic-stromal-lymphopoietin-enhances-lung-metastasis-through-an-alveolar-macrophage-dependent-mechanism
#17
Lauren Burkard-Mandel, Rachel O'Neill, Sean Colligan, Mukund Seshadri, Scott I Abrams
It is well-recognized that macrophages, which arise from circulating precursors, enhance tumor progression in patients and animal models. However, less is known regarding the role of tissue-resident macrophages in metastasis. Moreover, the identification of tumor factors which influence macrophage function in the metastatic niche remains incomplete. Here, we investigated one such cytokine known as thymic stromal lymphopoietin (TSLP). Our rationale to focus on TSLP was based on two non-overlapping findings; first, TSLP exacerbates asthma in part by altering the lung macrophage response and, secondly, TSLP is produced by certain mouse and human tumor systems, although its role in neoplasia remains understudied...
2018: Oncoimmunology
https://www.readbyqxmd.com/read/29713367/wentong-decoction-cures-allergic-bronchial-asthma-by-regulating-the-apoptosis-imbalance-of-eos
#18
Yue Yan, Hai-Peng Bao, Chun-Lei Li, Qi Shi, Yan-Hua Kong, Ting Yao, You-Lin Li
Background: Eosinophils (EOS) is one of the most important cells involved in the pathogenesis of chronic airway inflammation in asthma, and its apoptosis is part of the mechanisms of asthma. Therefore, this study aimed to observe the effect of Chinese medicine Wentong decoction (WTD) in EOS apoptosis in asthmatic rats. This work also explored the mechanism of WTD regulation in EOS apoptosis and provided a new target for clinical treatment of asthma. Methods: Asthmatic rats induced by ovalbumin were treated with WTD...
2018: Chinese Medicine
https://www.readbyqxmd.com/read/29709672/of-pleiotropy-and-trajectories-does-the-tgfb-pathway-link-childhood-asthma-and-copd
#19
Avery DeVries, Donata Vercelli
The study of developmental trajectories is where epigenetics truly shines. The "epi" in epi-genetics captures the fact that although epigenetic processes also preside over the maintenance and termination of gene expression, the unfolding and remodeling of chromatin architecture are especially critical to prepare genes for regulated transcription. These properties imply being on a path, a trajectory to events that will occur later on thanks to epigenetic programming. Thus, epigenetics is about timed and timely events...
April 27, 2018: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/29709669/does-reduced-zpbp2-expression-on-chromosome-17q21-protect-against-asthma
#20
Marina Miller, Christine Vuong, Meghan Farrell Garcia, Peter Rosenthal, Sudipta Das, Ning Weng, Alexa Pham, Yu Jin Kim, David H Broide
ZPBP2 is located on chromosome 17q21, a gene region highly linked to asthma. House dust mite (HDM) challenge in wild type mice induced expression of ZPBP2 in the lung epithelium. ZPBP2 deficient mice have reduced AHR to HDM challenge. Lack of ZPBP2 expression may thus protect against AHR and asthma.
April 27, 2018: Journal of Allergy and Clinical Immunology
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