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Asthma AND gene expression

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https://www.readbyqxmd.com/read/28649804/fibroblast-derived-exosomes-promote-epithelial-cell-proliferation-through-tgf-%C3%AE-2-signaling-pathway-in-severe-asthma
#1
Ikhlass Haj-Salem, Sophie Plante, Abdelilah S Gounni, Mahmoud Rouabhia, Jamila Chakir
BACKGROUND: Bronchial fibroblasts play a key role in airway remodeling in asthma. They regulate epithelial cell functions such as proliferation through growth factors, cytokines, chemokines and exosomes. The role of exosomes in the communication between epithelial cells and fibroblasts by vehiculing these mediators in asthma remains to be determined. OBJECTIVE: To evaluate the role of exosomes released by bronchial fibroblasts on epithelial cell proliferation in severe asthma...
June 26, 2017: Allergy
https://www.readbyqxmd.com/read/28649437/integrating-personalized-gene-expression-profiles-into-predictive-disease-associated-gene-pools
#2
Jörg Menche, Emre Guney, Amitabh Sharma, Patrick J Branigan, Matthew J Loza, Frédéric Baribaud, Radu Dobrin, Albert-László Barabási
Gene expression data are routinely used to identify genes that on average exhibit different expression levels between a case and a control group. Yet, very few of such differentially expressed genes are detectably perturbed in individual patients. Here, we develop a framework to construct personalized perturbation profiles for individual subjects, identifying the set of genes that are significantly perturbed in each individual. This allows us to characterize the heterogeneity of the molecular manifestations of complex diseases by quantifying the expression-level similarities and differences among patients with the same phenotype...
2017: NPJ Systems Biology and Applications
https://www.readbyqxmd.com/read/28638418/the-expression-of-stat3-and-stat5a-genes-in-severe-refractory-asthma
#3
Kayvan Saeedfar, Mehrdad Behmanesh, Esmaeil Mortaz, Mohammad Reza Masjedi
BACKGROUND: Despite being a high burden disorder, the pathogenesis of severe refractory asthma (SRA) is poorly understood. There are some evidences for the involvement of members of the signal transducer and activator of transcription (STAT) family, including STAT3 and STAT5a. Our study aimed to evaluate the gene expression of STAT3 and STAT5a in asthma and SRA to establish if there is an association. MATERIALS AND METHODS: Using quantitative real-time polymerase chain reactions (qRT-PCR), the transcript levels of STAT3 and STAT5a were evaluated in peripheral blood mononuclear lymphocytes (PBML) isolated from 13 patients with SRA, 14 with mild asthma, and 30 healthy volunteers...
2017: Tanaffos
https://www.readbyqxmd.com/read/28635548/th-17-regulatory-cytokines-il-21-il-23-and-il-6-enhance-neutrophil-production-of-il-17-cytokines-during-asthma
#4
Rabih Halwani, Asma Sultana, Alejandro Vazquez-Tello, Amer Jamhawi, Abeer A Al-Masri, Saleh Al-Muhsen
BACKGROUND: In a subset of severe asthma patients, chronic airway inflammation is associated with infiltration of neutrophils, Th-17 cells and elevated expression of Th-17-derived cytokines (e.g., interleukin [IL]-17, IL-21, IL-22). Peripheral neutrophils from allergic asthmatics are known to express higher IL-17 cytokine levels than those from healthy subjects, but the regulatory mechanisms involved are not well understood. We hypothesize that Th-17 regulatory cytokines could modulate IL-17 expression in neutrophils...
March 2, 2017: Journal of Asthma: Official Journal of the Association for the Care of Asthma
https://www.readbyqxmd.com/read/28629744/mannose-receptor-modulates-macrophage-polarization-and-allergic-inflammation-through-mir-511-3p
#5
Yufeng Zhou, Danh C Do, Faoud T Ishmael, Mario Leonardo Squadrito, Ho Man Tang, Ho Lam Tang, Man-Hsun Hsu, Lipeng Qiu, Changjun Li, Yongqing Zhang, Kevin G Becker, Mei Wan, Shau-Ku Huang, Peisong Gao
BACKGROUND: Mannose receptor (MRC1/CD206) has been suggested to mediate allergic sensitization and asthma to multiple glyco-allergens, including cockroach allergens. OBJECTIVE: Determine the existence of a protective mechanism through which MRC1 limits allergic inflammation through its intronic miR-511-3p. METHODS: We examined the MRC1-mediated cockroach allergen uptake by lung macrophages and lung inflammation using C57BL/6 wild-type (WT) and Mrc1(-/-) mice...
June 16, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28617138/targeting-transcription-factor-lysine-acetylation-in-inflammatory-airway-diseases
#6
Thea van den Bosch, Marcel Kwiatkowski, Rainer Bischoff, Frank J Dekker
Asthma and chronic obstructive pulmonary disease are inflammatory airway diseases for which alternative therapeutic strategies are urgently needed. Interestingly, HDAC inhibitors show anti-inflammatory effects in mouse models for these diseases. Here we explore underlying mechanisms that may explain these effects. In previous studies, effects of HDAC inhibitors on histone acetylation are often correlated with their effects on gene expression. However, effects of HDAC inhibitors on transcription factors and their acetylation status may be particularly important in explaining these effects...
June 15, 2017: Epigenomics
https://www.readbyqxmd.com/read/28608951/tumstatin-regulates-the-angiogenic-and-inflammatory-potential-of-airway-smooth-muscle-extracellular-matrix
#7
Louise Margaret Harkness, Markus Weckmann, Matthias Kopp, Tim Becker, Anthony Wayne Ashton, Janette Kay Burgess
The extracellular matrix (ECM) creates the microenvironment of the tissue; an altered ECM in the asthmatic airway may be central in airway inflammation and remodelling. Tumstatin is a collagen IV-derived matrikine reduced in the asthmatic airway wall that reverses airway inflammation and remodelling in small and large animal models of asthma. This study hypothesized that the mechanisms underlying the broad asthma-resolving effects of tumstatin were due to autocrine remodelling of the ECM. Neutrophils and endothelial cells were seeded on decellularized ECM of non-asthmatic (NA) or asthmatic (A) airway smooth muscle (ASM) cells previously exposed to tumstatin in the presence or absence of a broad matrix metalloproteinase inhibitor, Marimastat...
June 13, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28606589/regulatory-effects-of-interleukin-il-15-on-allergen-induced-airway-obstruction
#8
Sathisha Upparahalli Venkateshaiah, Xiang Zhu, Priya Rajavelu, Rituraj Niranjan, Murli Manohar, Alok K Verma, Joseph A Lasky, Anil Mishra
BACKGROUND: Airway obstruction is a physiologic feature of asthma and IL-15 may have an important role in the pathogenesis of asthma. OBJECTIVE: We tested the hypothesis that regulation of IL-15 is critical for the preservation of allergen-induced airway hyperresponsiveness (AHR), airway resistance and compliance in response to methacholine. METHODS: Airway inflammation, AHR, resistance and compliance were assessed in IL-15- gene deficient mice and IL-15 overexpressing mice in an allergen-induced murine model of asthma...
June 9, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28603280/dust-mite-allergen-specific-immunotherapy-increases-il4-dna-methylation-and-induces-der-p-specific-t-cell-tolerance-in-children-with-allergic-asthma
#9
Chuang-Ming Wang, Chia-Bin Chang, Michael Wy Chan, Zhi-Hong Wen, Shu-Fen Wu
Allergen-specific immunotherapy (allergen-SIT) is a highly effective treatment for children with allergic asthma (AA), an immune-mediated chronic disease leading to bronchial muscle hypertrophy and airway obstruction in response to specific allergens. T helper cells and secreted cytokines play important roles in the pathogenesis of asthma, and epigenetic modulation controls genes important for T cell development and cytokine expression. This study evaluated T helper cell-secreted cytokines and DNA methylation patterns in children treated with Dermatophagoides pteronyssinus (Der p) allergen-SIT...
June 12, 2017: Cellular & Molecular Immunology
https://www.readbyqxmd.com/read/28601047/role-of-epigenetics-in-the-pathogenesis-of-asthma
#10
Zahra Alizadeh, Esmaeil Mortaz, Ian Adcock, Mostafa Moin
Asthma is a complex, heterogeneous and chronic airway inflammatory disease with different clinical phenotypes caused by diverse triggers and pathophysiological mechanisms. Asthma heritability has been established in many genetic studies but it is evident that only genetic elements are not responsible for the development of asthma. Increasing rate of asthma incidence during past decades has implicated the role of epigenetics in development of asthma. Environmental factors perform as initiator signals through epigenetic mechanisms...
April 2017: Iranian Journal of Allergy, Asthma, and Immunology
https://www.readbyqxmd.com/read/28599122/respiratory-syncytial-virus-infection-compromises-asthma-tolerance-by-recruiting-interleukin-17a-producing-cells-via-ccr6-ccl20-signaling
#11
Tianyun Shi, Yanchao He, Wei Sun, Yi Wu, Ling Li, Zhijun Jie, Xiao Su
Asthma tolerance can be induced by breast-feeding or oral feeding with ovalbumin (OVA). Anergy or deletion of specific T cells and generation of T regulatory cells might contribute to this process. However, whether respiratory syncytial virus (RSV) infection would affect asthma tolerance is not very clear. Here, we first established asthma and oral tolerance mouse models and then analyzed airway hypersensitivity and asthma-related genes in the lung, CCR6-expressing IL-17A(+) cells in the lungs, hilar or mesenteric lymph nodes (HLN or MLN) among control, asthmatic, tolerized, RSV infection, and RSV-infected asthmatic and tolerized groups...
June 6, 2017: Molecular Immunology
https://www.readbyqxmd.com/read/28599074/beyond-epithelial-to-mesenchymal-transition-common-suppression-of-differentiation-programs-underlies-epithelial-barrier-dysfunction-in-mild-moderate-and-severe-asthma
#12
Lucas F Loffredo, Hiam Abdala-Valencia, Kishore R Anekalla, Lyda Cuervo-Pardo, Cara J Gottardi, Sergejs Berdnikovs
BACKGROUND: Epithelial barrier dysfunction is a central feature in the pathogenesis of allergic disease. Epithelial-to-mesenchymal transition (EMT) has been proposed as one mechanism afflicting barrier in asthma. However, genes and pathways involved in aberrant epithelial-mesenchymal signaling, and their relationship to asthma severity, are poorly understood. METHODS: We used unbiased gene network analysis to evaluate functional convergence in epithelial gene expression signatures across multiple public access transcriptomics datasets of human asthma, followed by text mining to evaluate functional marker relevance of discovered genes...
June 9, 2017: Allergy
https://www.readbyqxmd.com/read/28579377/dendritic-cells-conditioned-by-activin-a-induced-regulatory-t-cells-exhibit-enhanced-tolerogenic-properties-and-protect-against-experimental-asthma
#13
Maria Semitekolou, Ioannis Morianos, Aggelos Banos, Dimitris Konstantopoulos, Marina Adamou-Tzani, Tim Sparwasser, Georgina Xanthou
BACKGROUND: Previously, we have demonstrated that regulatory T (Treg) cells, induced by the cytokine activin-A, suppress Th2-mediated allergic responses and linked airway disease. Still, the effects of activin-A-induced Treg cells (act-A-iTreg cells) on the regulation of dendritic cell (DC)-driven allergic inflammation remain elusive. OBJECTIVE: Here, we investigated whether act-A-iTreg cells can modulate DC responses and endow them with enhanced tolerogenic functions...
June 1, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28579087/the-protective-role-of-helicobacter-pylori-neutrophil-activating-protein-in-childhood-asthma
#14
A Karakullukcu, H B Tokman, S Nepesov, M Demirci, S Saribas, S Vehid, R Caliskan, Z Taner, H Cokugras, T Ziver, S Demiryas, B Kocazeybek
BACKGROUND: Helicobacter pylori quantity and HP-NAP gene expression were evaluated in the faeces of healthy and asthmatic children. METHODS: H. pylori DNAs and RNAs were isolated from the stool samples of 92 asthmatic children (AC; 3-8 years) and 88 healthy controls (HC). Quantitative PCR was used to determine the quantity of H. pylori and HP-NAP expression relative to the 16S rRNA (reference gene). Gene expression was analysed using the delta delta-Ct method. RESULTS: H...
May 31, 2017: Allergologia et Immunopathologia
https://www.readbyqxmd.com/read/28576978/hox5-paralogous-genes-modulate-th2-cell-function-during-chronic-allergic-inflammation-via-regulation-of-gata3
#15
Catherine Ptaschinski, Steven M Hrycaj, Matthew A Schaller, Deneen M Wellik, Nicholas W Lukacs
Allergic asthma is a significant health burden in western countries, and continues to increase in prevalence. Th2 cells contribute to the development of disease through release of the cytokines IL-4, IL-5, and IL-13, resulting in increased airway eosinophils and mucus hypersecretion. The molecular mechanisms behind the disease pathology remain largely unknown. In this study we investigated a potential regulatory role for the Hox5 gene family, Hoxa5, Hoxb5, and Hoxc5, genes known to be important in lung development within mesenchymal cell populations...
June 2, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28574721/a-multi-omics-approach-to-identify-genes-associated-with-childhood-asthma-risk-and-morbidity
#16
Erick Forno, Ting Wang, Qi Yan, John Brehm, Edna Acosta-Perez, Angel Colon-Semidey, Maria Alvarez, Nadia Boutaoui, Michelle M Cloutier, John F Alcorn, Glorisa Canino, Wei Chen, Juan C Celedón
RATIONALE: Childhood asthma is a complex disease. In this study, we aim to identify genes associated with childhood asthma through a multi-omics "vertical" approach that integrates multiple analytical steps using linear and logistic regression models. METHODS: In a case-control study of childhood asthma in Puerto Ricans (n=1127), we use adjusted linear or logistic regression models to evaluate associations between several analytical steps of omics data, including genome-wide (GW) genotype data, GW methylation, GW expression profiling, cytokine levels, asthma-intermediate phenotypes, and asthma status...
June 2, 2017: American Journal of Respiratory Cell and Molecular Biology
https://www.readbyqxmd.com/read/28559992/delivery-of-adipose-derived-mesenchymal-stem-cells-attenuates-airway-responsiveness-and-inflammation-in-a-mouse-model-of-ovalbumin-induced-asthma
#17
Ranran Dai, Jia Liu, Songbai Cai, Chengxiao Zheng, Xin Zhou
Adipose-derived mesenchymal stem cells (ADMSCs) possess immunomodulation property, yet their therapeutic potential in asthma is unclear. This study aimed to explore the effects of ADMSCs on airway hyperresponsiveness and inflammation in ovalbumin (OVA)-induced asthma models. The underlying mechanism(s) was also examined. BALB/c mice were sensitized with OVA on days 0, 7, and 14, followed by 8-week OVA challenge from day 22. ADMSCs were injected via tail vein on day 21. Animals were measured for airway responsiveness, lung pathology, IgE and cytokine levels in serum, cell composition in bronchoalveolar lavage fluid (BALF), gene expression in the lung, and regulatory T cells (Tregs)...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28553015/interluekin-35-in-asthma-and-its-potential-as-an-effective-therapeutic-agent
#18
REVIEW
Peng Gao, Zhenzhong Su, Xuejiao Lv, Jie Zhang
Interleukin- (IL-) 35 is a member of the IL-12 cytokine family and a heterodimeric protein formed by Epstein-Barr-induced gene 3 (EBI3) and IL-12p35. Emerging evidence shows that IL-35 is a key player in the regulation of cellular communication, differentiation, and inflammation. Altered IL-35 expression has been found in disease conditions such as cancer, rheumatoid arthritis, and, more recently, asthma. In cancer, IL-35 is involved in the regulation of tumorigenesis, cancer progression, and metastasis. In rheumatoid arthritis, IL-35 acts as a negative regulator of inflammation...
2017: Mediators of Inflammation
https://www.readbyqxmd.com/read/28552993/rhinovirus-infection-results-in-stronger-and-more-persistent-genomic-dysregulation-evidence-for-altered-innate-immune-response-in-asthmatics-at-baseline-early-in-infection-and-during-convalescence
#19
Peter W Heymann, Huyen-Tran Nguyen, John W Steinke, Ronald B Turner, Judith A Woodfolk, Thomas A E Platts-Mills, Lisa Martin, Hua He, Jocelyn Biagini Myers, Mark Lindsey, Umasundari Sivaprasad, Mario Medvedovic, Naim Mahi, Holliday Carper, Deborah D Murphy, James Patrie, Gurjit K Khurana Hershey
BACKGROUND: Rhinovirus (HRV) is associated with the large majority of virus-induced asthma exacerbations in children and young adults, but the mechanisms remain poorly defined. METHODS: Asthmatics and non-asthmatic controls were inoculated with HRV-A16, and nasal epithelial samples were obtained 7 days before, 36 hours after, and 7 days after viral inoculation. RNA was extracted and subjected to RNA-seq analysis. RESULTS: At baseline, 57 genes were differentially expressed between asthmatics and controls, and the asthmatics had decreased expression of viral replication inhibitors and increased expression of genes involved in inflammation...
2017: PloS One
https://www.readbyqxmd.com/read/28552588/vitamin-d-supplementation-during-pregnancy-effect-on-the-neonatal-immune-system-in-a-randomized-controlled-trial
#20
Eve Hornsby, Paul E Pfeffer, Nancy Laranjo, William Cruikshank, Marina Tuzova, Augusto A Litonjua, Scott T Weiss, Vincent J Carey, George O'Connor, Catherine Hawrylowicz
BACKGROUND: Programming of the immune system during fetal development can influence asthma-related risk factors and outcomes in later life. Vitamin D is a well-recognized immune modulator, and deficiency of this nutrient during pregnancy is hypothesized to influence disease development in offspring. OBJECTIVE: We sought to investigate the effect on neonatal immunity of maternal supplementation with 4400 IU/d vitamin D3 during the second and third trimesters of pregnancy by using a subset of cord blood samples from a randomized, double-blind, placebo-controlled clinical trial (the Vitamin D Antenatal Asthma Reduction Trial)...
May 16, 2017: Journal of Allergy and Clinical Immunology
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