keyword
MENU ▼
Read by QxMD icon Read
search

vessel normalization

keyword
https://www.readbyqxmd.com/read/29656244/human-organoid-cultures-transformative-new-tools-for-human-virus-studies
#1
REVIEW
Sasirekha Ramani, Sue E Crawford, Sarah E Blutt, Mary K Estes
Studies of human infectious diseases have been limited by the paucity of functional models that mimic normal human physiology and pathophysiology. Recent advances in the development of multicellular, physiologically active organotypic cultures produced from embryonic and pluripotent stem cells, as well as from stem cells isolated from biopsies and surgical specimens are allowing unprecedented new studies and discoveries about host-microbe interactions. Here, we summarize recent developments in the use of organoids for studying human viral pathogens, including intestinal infections with human rotavirus, norovirus, enteroviruses and adenoviruses (intestinal organoids and enteroids), neuronal infections with Zika virus (cerebral organoids) and respiratory infections with respiratory syncytial virus in (lung bud organoids)...
April 12, 2018: Current Opinion in Virology
https://www.readbyqxmd.com/read/29653462/docosahexaenoic-acid-reversed-atherosclerotic-changes-in-human-endothelial-cells-induced-by-palmitic-acid-in-vitro
#2
Saeede Karbasforush, Alireza Nourazarian, Masoud Darabi, Reza Rahbarghazi, Fatemeh Khaki-Khatibi, Çıgır Biray Avci, Leila Salimi, Bakiye Goker Bagca, Tanaz Novin Bahador, Aysa Rezabakhsh, Majid Khaksar
Abnormal activity of atherosclerotic endothelial cells paving luminal surface of blood vessels has been described in many diseases. It has been reported that natural polyunsaturated fatty acids such as docosahexaenoic acid exert therapeutic effects in atherosclerotic condition. Human umbilical vein endothelial cells were treated with 1mM palmitic acid for 48 hours and exposed to 40μM docosahexaenoic acid for the next 24 hours. Real-time polymerase chain reaction analysis was used to measure the expression of PTX3, iNOS, and eNOS...
April 13, 2018: Cell Biochemistry and Function
https://www.readbyqxmd.com/read/29652661/combined-brain-and-heart-magnetic-resonance-imaging-in-systemic-vasculitides-fiction-or-real-need
#3
REVIEW
Sophie I Mavrogeni, George Kitas, Hildo J Lamb, Klearchos Psychoyios, Theodoros Dimitroulas, Loukia Koutsogeorgopoulou, Kyriaki Boki, Vasiliki Vartela, Genovefa Kolovou, George Markousis-Mavrogenis, Cees G Kallenberg, Loic Guillevin, Dimitrios Vassilopoulos
Systemic vasculitides (SVs) is a group of diseases characterised by inflammation/necrosis of the blood vessel wall in various organs. Simultaneous brain and heart involvement is a cause of increased morbidity/mortality in SV. We aimed to present evidence of concurrent brain/heart involvement in SV and the role of a combined brain/heart magnetic resonance imaging (MRI) in their risk stratification. Cerebral vasculitis (CV) can be presented as focal deficits, seizures, headache, neuropsychiatric manifestations or cognitive dysfunction and cardiovascular disease (CVD) as myocardial/vascular inflammation, perfusion/function defects and fibrosis...
April 10, 2018: Clinical and Experimental Rheumatology
https://www.readbyqxmd.com/read/29651567/the-origin-of-the-medial-femoral-circumflex-artery-lateral-femoral-circumflex-artery-and-obturator-artery
#4
REVIEW
M Zlotorowicz, M Czubak-Wrzosek, P Wrzosek, J Czubak
INTRODUCTION: The most significant blood vessel supplying the hip joint is the medial femoral circumflex artery (MFCA). MFCA with lateral femoral circumflex artery (LFCA) are the first branches of the deep femoral artery (DFA) or they originate directly from the common femoral artery (CFA) or superficial femoral artery (SFA). PURPOSE AND METHODS: We analyzed 100 CT angiogram of the hip region [72 men, 28 women; mean age 46.4 (14-80)] to assess the frequency of each type of division of the MFCA and LFCA from either the DFA or directly from the CFA or SFA...
April 12, 2018: Surgical and Radiologic Anatomy: SRA
https://www.readbyqxmd.com/read/29651419/management-of-glioblastoma-multiforme-in-a-patient-treated-with-ketogenic-metabolic-therapy-and-modified-standard-of-care-a-24-month-follow-up
#5
Ahmed M A Elsakka, Mohamed Abdel Bary, Eman Abdelzaher, Mostafa Elnaggar, Miriam Kalamian, Purna Mukherjee, Thomas N Seyfried
Few advances have been made in overall survival for glioblastoma multiforme (GBM) in more than 40 years. Here, we report the case of a 38-year-old man who presented with chronic headache, nausea, and vomiting accompanied by left partial motor seizures and upper left limb weakness. Enhanced brain magnetic resonance imaging revealed a solid cystic lesion in the right partial space suggesting GBM. Serum testing revealed vitamin D deficiency and elevated levels of insulin and triglycerides. Prior to subtotal tumor resection and standard of care (SOC), the patient conducted a 72-h water-only fast...
2018: Frontiers in Nutrition
https://www.readbyqxmd.com/read/29651339/comparison-of-optical-coherence-tomography-angiography-and-laser-speckle-flowgraphy-for-the-diagnosis-of-normal-tension-glaucoma
#6
Asuka Takeyama, Kyoko Ishida, Ayako Anraku, Masahiro Ishida, Goji Tomita
Purpose: To compare optical coherence tomography angiography (OCT-A) and laser speckle flowgraphy (LSFG) for the diagnosis of normal-tension glaucoma (NTG). Methods: Twenty-eight eyes of 28 patients with NTG and 25 eyes of 25 normal subjects matched for age, refractive errors, systemic blood pressure, and central corneal thickness were evaluated. OCT-A was used to measure whole image vessel density, inside disc vessel density, and peripapillary vessel density; using LSFG, mean blur rate (MBR) inside the whole optic nerve head (ONH) area (MBRA ), and MBR of the vessel area (MBRV ) and tissue area (MBRT ) inside the ONH, were determined...
2018: Journal of Ophthalmology
https://www.readbyqxmd.com/read/29650228/visualization-of-proliferative-vascular-endothelial-cells-in-tumors-in-vivo-by-imaging-their-partner-of-sld5-1-promoter-activity
#7
Daishi Yamakawa, Weizhen Jia, Hiroyasu Kidoya, Shoko Hosojima, Miku Torigata, Li Zhang, Nobuyuki Takakura
Vascular endothelial cells (ECs) isolated from tumors characteristically express certain genes. It has recently been suggested that tumor vessel normalization facilitates effective drug delivery into tumors; however, how tumor vessel normalization can be recognized on the basis of the molecules expressed by tumor ECs is not clearly defined. The degree of cell proliferation is an important indicator to characterize the condition of the ECs. Herein, we generated transgenic mice expressing enhanced green fluorescent protein (EGFP) under the transcriptional control of the DNA replication factor partner of Sld5-1 (PSF1; official name GINS1) promoter to assess whether active ECs can be distinguished from dormant ECs...
April 2, 2018: American Journal of Pathology
https://www.readbyqxmd.com/read/29644895/%C3%AE-1-subunit-of-the-calcium-sensitive-potassium-channel-modulates-the-pulmonary-vascular-smooth-muscle-cell-response-to-hypoxia
#8
Elizabeth A Barnes, Lori Lee, Shayna L Barnes, Robert Brenner, Cristina M Alvira, David N Cornfield
Accessory subunits associated with the calcium-sensitive potassium channel (BKCa ), a major determinant of vascular tone, confer functional and anatomical diversity. The β1 subunit increases Ca2+ -, and voltage-sensitivity of the BKCa channel and is expressed exclusively in smooth muscle cells (SMC). Evidence supporting the physiologic significance of the β1 subunit includes the observations that murine models with deletion of the β1 subunit are hypertensive and that humans with a gain-of-function β1 mutation are at decreased risk of diastolic hypertension, However, whether the β1 subunit of the BKCa channel contributes to the low tone that characterizes the normal pulmonary circulation or modulates the pulmonary vascular response to hypoxia remains unknown...
April 12, 2018: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/29643396/effects-of-muscle-relaxants-on-ischaemia-damage-in-skeletal-muscle
#9
Thomas Ledowski, Simone Nißler, Manuel Wenk, Esther M Pogatzki-Zahn, Daniel Segelcke
Muscle ischaemia is frequently induced intraoperatively by i.e. a surgical tourniquet or during the re-grafting phase of a free muscle transplant. The resulting muscle cell damage may impact on postoperative recovery. Neuromuscular paralysis may mitigate the effects of ischaemia. After ethics approval, 25 male Sprague-Dawley rats were anaesthetized and randomly assigned to 1 of 4 groups: Sham operation, treatment with normal saline, treatment with rocuronium (muscle relaxant) 0.6 or 1 mg kg-1 , respectively...
April 11, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29643120/ephrinb2-ephb4-signaling-regulates-non-sprouting-angiogenesis-by-vegf
#10
Elena Groppa, Sime Brkic, Andrea Uccelli, Galina Wirth, Petra Korpisalo-Pirinen, Maria Filippova, Boris Dasen, Veronica Sacchi, Manuele Giuseppe Muraro, Marianna Trani, Silvia Reginato, Roberto Gianni-Barrera, Seppo Ylä-Herttuala, Andrea Banfi
Vascular endothelial growth factor (VEGF) is the master regulator of angiogenesis, whose best-understood mechanism is sprouting. However, therapeutic VEGF delivery to ischemic muscle induces angiogenesis by the alternative process of intussusception, or vascular splitting, whose molecular regulation is essentially unknown. Here, we identify ephrinB2/EphB4 signaling as a key regulator of intussusceptive angiogenesis and its outcome under therapeutically relevant conditions. EphB4 signaling fine-tunes the degree of endothelial proliferation induced by specific VEGF doses during the initial stage of circumferential enlargement of vessels, thereby limiting their size and subsequently enabling successful splitting into normal capillary networks...
April 11, 2018: EMBO Reports
https://www.readbyqxmd.com/read/29628894/insulin-receptor-substrate-2-controls-insulin-mediated-vasoreactivity-and-perivascular-adipose-tissue-function-in-muscle
#11
Alexander H Turaihi, Wineke Bakker, Victor W M van Hinsbergh, Erik H Serné, Yvo M Smulders, Hans W M Niessen, Etto C Eringa
Introduction: Insulin signaling in adipose tissue has been shown to regulate insulin's effects in muscle. In muscle, perivascular adipose tissue (PVAT) and vascular insulin signaling regulate muscle perfusion. Insulin receptor substrate (IRS) 2 has been shown to control adipose tissue function and glucose metabolism, and here we tested the hypothesis that IRS2 mediates insulin's actions on the vessel wall as well as the vasoactive properties of PVAT. Methods: We studied PVAT and muscle resistance arteries (RA) from littermate IRS2+/+ and IRS2-/- mice and vasoreactivity by pressure myography, vascular insulin signaling, adipokine expression, and release and PVAT morphology...
2018: Frontiers in Physiology
https://www.readbyqxmd.com/read/29627628/early-administration-of-hypertonic-hyperoncotic-hydroxyethyl-starch-hyperhes-improves-cerebral-blood-flow-and-outcome-after-experimental-subarachnoid-hemorrhage-in-rats
#12
Nadine Lilla, Christoph Rinne, Judith Weiland, Thomas Linsenmann, Thomas Westermaier
OBJECTIVE: Early cytotoxic brain edema may be one decisive factor which maintains a vicious of cerebral malperfusion after subarachnoid hemorrhage (SAH). In addition, endothelial cell swelling may be an independent factor restricting cerebral blood flow (CBF) in a very early stage after SAH. Immediate and aggressive treatment may be able to restore CBF in this critical period. MATERIALS AND METHODS: Male Sprague-Dawley rats were subjected to SAH by the endovascular filament model and treated by a bolus of hyperoncotic-hypertonic hydroxyethyl starch (HyperHES 4 ml/kg body weight) immediately after vessel perforation and 150 min later (n = 12) or by the same amount of normal saline (n = 9)...
April 5, 2018: World Neurosurgery
https://www.readbyqxmd.com/read/29627228/establishment-of-an-abnormal-vascular-patterning-model-in-the-mouse-retina
#13
Akane Morita, Shohei Sawada, Asami Mori, Shiho Arima, Kenji Sakamoto, Tohru Nagamitsu, Tsutomu Nakahara
Abnormalities in retinal blood vessels and neuronal function persist in eyes undergoing retinopathy of prematurity. In this study, we examined morphological and functional changes in retinal blood vessels and neurons in mice that had undergone short-term interruption of retinal vascular development through inhibition of vascular endothelial growth factor (VEGF) signaling. In mice treated with the VEGF receptor tyrosine kinase inhibitor KRN633 on postnatal day (P) 0 and 1, the vascular density in the retinal surface increased by P12, but development of deep retinal vascular plexus and choroidal vasculature was delayed until P14...
March 14, 2018: Journal of Pharmacological Sciences
https://www.readbyqxmd.com/read/29626124/in-vivo-characterization-of-four-68-ga-labeled-multimeric-rgd-peptides-to-image-%C3%AE-v-%C3%AE-3-integrin-expression-in-two-human-tumor-xenograft-mouse-models
#14
Daphne Lobeek, Gerben M Franssen, Michelle T Ma, Hans-Jürgen Wester, Clemens Decristoforo, Wim J G Oyen, Otto C Boerman, Samantha Y A Terry, Mark Rijpkema
Rationale: αv β3 integrins play an important role in angiogenesis and cell migration of cancers. They are highly expressed on activated endothelial cells of newly formed blood vessels. Here, we compare the targeting characteristics of four68 Ga-labeled multimeric cyclic arginine-glycine-aspartate RGD-based tracers in an αv β3 integrin-expressing tumor model and in a tumor model where αv β3 integrin is expressed solely on the neovasculature. Methods: Female BALB/c nude mice were subcutaneously injected with SK-RC-52 (αv β3 integrin-positive) or FaDu (αv β3 integrin-negative) tumor cells...
April 6, 2018: Journal of Nuclear Medicine: Official Publication, Society of Nuclear Medicine
https://www.readbyqxmd.com/read/29625452/inner-retinal-microvasculature-damage-correlates-with-outer-retinal-disruption-during-remission-in-beh%C3%A3-et-s-posterior-uveitis-by-optical-coherence-tomography-angiography
#15
Dan Cheng, Meixiao Shen, Xiran Zhuang, Dan Lin, Mali Dai, Sisi Chen, Fan Lu, Yuqin Wang
Purpose: To quantify the inner retinal vascular changes that occur in the superficial and deep layers in patients with Behçet's disease (BD) in remission using optical coherence tomography angiography (OCTA) and to evaluate the associations with outer retinal structure. Methods: Nineteen eyes from 19 patients with BD in remission were enrolled, including 10 eyes with less than five ocular attacks (n < 5) and nine eyes with five or more attacks (n ≥ 5). The foveal avascular zone (FAZ) and global and regional vessel density (VD) in both layers were compared between BD eyes and normal eyes...
March 1, 2018: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29621956/ovarian-hyperstimulation-reduces-vascular-endothelial-growth-factor-a-during-uterine-receptivity
#16
Kevin Danastas, Camilla M Whittington, Samson N Dowland, Valery Combes, Christopher R Murphy, Laura A Lindsay
The angiogenic factor vascular endothelial growth factor-A (VEGFA) plays a critical role during early pregnancy in many species including the rat, and any alterations in VEGFA levels can severely impact blastocyst implantation rates. The rat ovarian hyperstimulation (OH) model is useful in studying how the induction of superovulation affects VEGFA levels and endometrial receptivity to blastocyst implantation. The present study shows that the major isoform in the rat uterus, Vegf188 , is reduced at the time of receptivity in OH compared to normal pregnancy, whereas there is no change in Vegf164 and Vegf120 messenger RNA (mRNA)...
January 1, 2018: Reproductive Sciences
https://www.readbyqxmd.com/read/29620183/puerarin-regulates-neovascular-glaucoma-through-pigment-epithelium%C3%A2-derived-growth-factor%C3%A2-induced-nf%C3%A2-%C3%AE%C2%BAb-signaling-pathway
#17
Hui-Yu Wei, Ya-Jie Zhang, Shao-Zhen Zhao
Neovascular glaucoma is an ophthalmic disease and a potentially blinding secondary glaucoma caused by the formation of abnormal new blood vessels on the iris, which can prevent the normal drainage of water from the anterior segment of the eye. Evidence from China has suggested that puerarin benefits many diseases including myocardial infarction, stable angina, cerebral ischemia and glaucoma in a clinical setting. In the present study, the aim was to investigate the efficacies of puerarin on neovascular glaucoma in a mouse model...
March 28, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29618124/microsurgical-resection-of-a-large-intraventricular-trigonal-tumor-3-dimensional-operative-video
#18
Chun-Yu Cheng, Rakshith Shetty, Laligam N Sekhar
A 62-yr-old woman presented with incidentally detected left trigonal mass by magnetic resonance imaging (MRI) performed during workup for left-sided hearing loss and vertigo of 5-yr duration. Due to persistent dizziness, headache, and progressive enlargement of the tumor in follow-up scans, operation was planned. Because the tumor extended superiorly, a superior parietal lobule approach was selected.She underwent a left parietal craniotomy. A strip electrode was used to localize the motor and sensory regions, and neuronavigation was used to confirm the entry site...
March 30, 2018: Operative Neurosurgery (Hagerstown, Md.)
https://www.readbyqxmd.com/read/29617192/optical-clearing-of-the-pancreas-for-visualization-of-mature-%C3%AE-cells-and-vessels-in-mice
#19
Wataru Nishimura, Asako Sakaue-Sawano, Satoru Takahashi, Atsushi Miyawaki, Kazuki Yasuda, Yasuko Noda
Glucose metabolism is regulated by insulin, which is produced from β-cells in the pancreas. Because insulin is secreted into vessels in response to blood glucose, vascular structures of the pancreas, especially the relationship between vessels and β-cells, are important for physiological and pathological glucose metabolism. Here, we developed a system to visualize vessels surrounding mature β-cells expressing transcription factor MafA in a three-dimensional manner. Optical clearing of the pancreas prevented light scattering of fluorescence driven by the bacterial artificial chromosome (BAC)-mafA promoter in β-cells...
April 4, 2018: Islets
https://www.readbyqxmd.com/read/29616175/single-lymphaticovenular-anastomosis-for-early-stage-lower-extremity-lymphedema-treated-by-the-superior-edge-of-the-knee-incision-method
#20
Yukio Seki, Akiyoshi Kajikawa, Takumi Yamamoto, Takayuki Takeuchi, Takahiro Terashima, Norimitsu Kurogi
Background: Surgical treatment of lower extremity lymphedema (LEL) remains challenging. Application of the superior-edge-of-the-knee incision method for lymphaticovenular anastomosis (LVA) is reported to have a strong therapeutic effect in patients with LEL because lymph-to-venous flow at the anastomosis is enhanced by knee joint movement during normal walking. We investigated whether a single LVA created by this method is adequate for early LEL. Methods: The study involved 10 patients with LEL characterized by stage 2 or 3 leg dermal backflow and treated by a single LVA at the thigh via the superior-edge-of-the-knee incision method...
February 2018: Plastic and Reconstructive Surgery. Global Open
keyword
keyword
31454
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"