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Tian Shen, Delin Gu, Yihua Zhu, Junwei Shi, Dongsheng Xu, Xingjian Cao
BACKGROUND: The morphological changes in activated neutrophils associated with antituberculosis drugs can be measured by volume, conductivity, and scatter (VCS) technology on the Coulter LH750 hematology analyzer. We conducted the current study to further validate the clinical usefulness of the neutrophil VCS parameters in predicting drug-induced neutropenia. METHODS: Peripheral blood samples were collected from 52 patients with drug-induced neutropenia, 309 patients without any abnormal CBC, and 237 healthy controls...
August 1, 2016: Clinica Chimica Acta; International Journal of Clinical Chemistry
T Abiramalatha, S Santhanam, J J Mammen, G Rebekah, M P Shabeer, J Choudhury, S C Nair
OBJECTIVE: The objective of this study was to determine changes in neutrophil volume conductivity scatter (VCS) parameters and their distribution widths (DW) in neonatal sepsis and to estimate their optimal cutoff levels using receiver operating characteristic (ROC) curves. STUDY DESIGN: In a cohort of neonates evaluated for sepsis, blood counts and blood culture were performed initially, with repeat counts and C-reactive protein (CRP) done after 24 to 48 h. Neutrophil VCS parameters from both the initial and repeat blood counts were analyzed...
September 2016: Journal of Perinatology: Official Journal of the California Perinatal Association
Pooja K Suresh, Jessica Minal, Purnima S Rao, Kirthinath Ballal, Hanaganahalli B Sridevi, Mahesha Padyana
INTRODUCTION: Complete blood picture is the first and foremost investigation ordered for evaluation of acute infectious processes which require early and prompt intervention. With the advent of automated Coulter (®) Haematology analysers, the Volume, Conductivity & Scatter parameters of the leucocytes could be analysed for the early detection of the infections. AIM: To evaluate the clinical usefulness of Volume, Conductivity & Scatter parameters of neutrophils & monocytes in predicting the onset of acute bacterial infections...
January 2016: Journal of Clinical and Diagnostic Research: JCDR
Jan J van Wingerden, Mario Maas, Richard L Braam, Bas A de Mol
PURPOSE: Poststernotomy mediastinitis (PSM), the severe chest wall and mediastinal infection that may arise at any time after a sternotomy, causes significant morbidity and mortality globally. Late recognition and diagnosis are the major contributors to a poor outcome. This review focuses on recent advances in diagnosing PSM (particularly after cardiovascular surgery) at the earliest opportunity--in the emergency department. RECENT FINDINGS: Morbidity and mortality of PSM, especially when associated with numerous other complications, remain unaltered high...
March 2016: American Journal of Emergency Medicine
Ying Xing, Chenxue Qu, Meiling Wang, Dingling Wang, Yinan Song, Run Wang, Ying Zhu, Jinmei Li
OBJECTIVE: To explore the cell population data (CPD) parameters of DxH800 hematology analyzer in predicting engraftment after hematopoietic stem cell transplantation. METHODS: Data from 109 patients who underwent hematopoietic stem cell transplantation at Peking University First Hospital between July 2013 and September 2014 were selected, all CBC data including CPD parameters from the beginning to engraftment (absolute neutrophil count > 500/µl) were gathered and analyzed to find the early markers of myeloid engraftment and establish the cut-off value...
July 28, 2015: Zhonghua Yi Xue za Zhi [Chinese medical journal]
N Zhou, L Liu, D Li, Q Zeng, X Song
Bacterial infections increased greatly in cancer patients who accepted cytotoxic chemotherapeutics. VCS parameters of neutrophils were reported to be an indicator for acute bacterial infection accompanied by increased WBC counts. Here we explored the possibility of VCS parameters of neutrophils, monocytes and lymphocytes in indicating the local bacterial infection in cancer patients. A total of 310 cancer patients and 90 healthy controls were retrospectively analyzed, and 190 of them were diagnosed as acute local bacterial infection...
January 2016: European Journal of Clinical Microbiology & Infectious Diseases
G P J van Hout, W W van Solinge, C M Gijsberts, M P J Teuben, P H C Leliefeld, M Heeres, F Nijhoff, S de Jong, L Bosch, S C A de Jager, A Huisman, P R Stella, G Pasterkamp, L J Koenderman, I E Hoefer
Myocardial infarction (MI) induces an inflammatory response in which neutrophils fulfill a prominent role. Mean neutrophil volume (MNV) represents the average size of the circulating neutrophil population. Our goal was to determine the effect of MI on MNV and investigate the mechanisms behind MNV elevation. MNV of 84 MI patients was compared with the MNV of 209 stable angina patients and correlated to simultaneously measured CK levels. Fourteen pigs were subjected to temporary coronary balloon occlusion and blood was sampled at multiple time points to measure MNV...
November 2015: Basic Research in Cardiology
J Lee, S Y Kim, W Lee, K Han, I K Sung
INTRODUCTION: Cell population data (CPD) describe physical parameters of white blood cell subpopulations and are reported to be of some value in the diagnosis of sepsis in neonates. Before using the CPD for diagnosing sepsis, the baseline features of the CPD distribution in healthy neonates should be clarified. The aim of this study was to compare the CPD distributions of healthy neonates and other age groups and to identify perinatal factors that are associated with changes in the CPD distribution of healthy neonates...
October 2015: International Journal of Laboratory Hematology
Siyu He, Hongmin Zhang, Susu Liu, Hui Liu, Guoming Chen, Yanting Xie, Junjie Zhang, Shengtao Sun, Zhijie Li, Liya Wang
As an important immunoregulatory cell type, the role of γδ T cells in fungal keratitis (FK) is unclear. We observed the distribution of γδ T cells in infected corneas in vivo by two-photon microscopy. The γδ T cells were depleted by neutralizing antibodies. The cytokine expression profile was obtained by protein arrays to determine the cytokines regulated by γδ T cells. ICAM-1, MIP-2 and IL-17A were evaluated by ELISA assays to confirm the role of γδ T cells in FK. We counted the number of neutrophils, evaluated the volume of fungal hyphae and analyzed the manifestation of the disease...
June 2015: Experimental Eye Research
Marc A Sze, Soraya Utokaparch, W Mark Elliott, James C Hogg, Richard G Hegele
OBJECTIVES: The present study assesses the relationship between contents of GD1 (glycerol dehydratase)-positive Lactobacillus, presence of Lactobacillus and the inflammatory response measured in host lung tissue in mild to moderate chronic obstructive pulmonary disease (COPD). We hypothesise that there will be a loss of GD1 producing Lactobacillus with increasing severity of COPD and that GD1 has anti-inflammatory properties. SETTING: Secondary care, 1 participating centre in Vancouver, British Columbia, Canada...
2015: BMJ Open
Yihua Zhu, Xingjian Cao, Kexia Zhang, Wei Xie, Dongsheng Xu, Chongjun Zhong
BACKGROUND: The Coulter LH750 (Beckman Coulter, Brea, CA) analyzer can determine intrinsic biophysical properties of white blood cell (WBC), known as cell population data. Previous studies have shown that mean neutrophil volume (MNV) was significantly increased in postsurgical patients with bacterial infection. To further validate its potential clinical usefulness, we investigate the changes in MNV before and after surgery, called ΔMNV. We also compare the ΔMNV with procalcitonin (PCT) and C-reactive protein (CRP) in terms of diagnostic sensitivity and specificity for postsurgical bacterial infection...
July 2014: Journal of Clinical Laboratory Analysis
I H Celik, G Demirel, D Sukhachev, O Erdeve, U Dilmen
INTRODUCTION: Neonatal sepsis remains an important clinical syndrome despite advances in neonatology. Current hematology analyzers can determine cell volume (V), conductivity for internal composition of cell (C) and light scatter for cytoplasmic granularity and nuclear structure (S), and standard deviations which are effective in the diagnosis of sepsis. Statistical models can be used to strengthen the diagnosis. Effective modeling of molecular activity (EMMA) uses combinatorial algorithm of the selection parameters for regression equation based on modified stepwise procedure...
February 2013: International Journal of Laboratory Hematology
Istemi H Celik, Gamze Demirel, Hatice T Aksoy, Omer Erdeve, Ece Tuncer, Zeynep Biyikli, Ugur Dilmen
INTRODUCTION: The Coulter LH780 hematology analyzer can evaluate mean neutrophil volume (MNV), conductivity (MNC), scatter (MNS), and distribution width (DW). We sought to investigate the value of volume, conductivity, and scatter (VCS) parameters in diagnosis and treatment efficacy of neonatal sepsis. RESULTS: We observed significant increases in MNV, volume distribution width (VDW), conductivity distribution width (CDW), and significant decreases in MNC and MNS in septic newborns...
January 2012: Pediatric Research
Y Zhu, X Cao, Y Chen, K Zhang, Y Wang, K Yuan, D Xu
INTRODUCTION: The Coulter LH750 analyzer can determine white blood cell count (WBC) with differentials and determine their intrinsic biophysical properties, known as cell population data (CPD). Previous studies demonstrated that neutrophil CPD, mean neutrophil volume (MNV), and neutrophil volume distribution width (NDW) were significantly increased during bacterial infection. This study investigated the clinical usefulness of MNV/NDW to differentiate postoperative infection. METHODS: Cell population data from 212 controls, 135 patients without postsurgical infection, and 62 with infection were studied...
June 2012: International Journal of Laboratory Hematology
C Risch, P Medina, U E Nydegger, Z Bahador, T Brinkmann, P Von Landenberg, M Risch, L Risch
INTRODUCTION: After measurement of the mean volumes of leukocyte subpopulations as well as the distribution widths (DW) of these volumes has become available, we investigated whether such morphometric leukocyte parameters are associated with a commonly used marker of cobalamin deficiency, i.e., holotranscobalamin (HoloTC). Further, we determined reference intervals for these parameters in an elderly population. METHODS: Consecutive subjectively healthy and volunteering individuals ≥60 years were included...
April 2012: International Journal of Laboratory Hematology
Thommie Karlsson, Michael Glogauer, Richard P Ellen, Vesa-Matti Loitto, Karl-Eric Magnusson, Marco A O Magalhães
Neutrophils are of prime importance in the host innate defense against invading microorganisms by using two primary mechanisms-locomotion toward and phagocytosis of the prey. Recent research points to pivotal roles for water channels known as AQPs in cell motility. Here, we focused on the role of AQP9 in chemoattractant-induced polarization and migration of primary mouse neutrophils and neutrophil-like HL60 cells. We found that AQP9 is phosphorylated downstream of fMLFR or PMA stimulation in primary human neutrophils...
November 2011: Journal of Leukocyte Biology
Khalil M Charafeddine, Amira M Youssef, Rami A R Mahfouz, Doja S Sarieddine, Rose T Daher
BACKGROUND: The aim of this study was to assess the use of neutrophil distribution width (NDW) and to compare it to C-reactive protein (CRP) and procalcitonin (PCT), in the detection of early sepsis in the intensive care unit. METHODS: Subjects (N = 166) were divided into 4 groups: healthy, acute inflammatory non-infectious (AINI), localized infection, and systemic infection, according to clinical history and cultures. NDW, CRP, and PCT were compared among the different groups using multivariate analysis of variance (MANOVA)...
October 2011: Scandinavian Journal of Infectious Diseases
Soie Chung, Ji-Eun Kim, Seungman Park, Kyou-Sup Han, Hyun Kyung Kim
Monocyte and neutrophil activation occur during microvascular disturbance of disseminated intravascular coagulation (DIC). This study investigated the diagnostic and prognostic value of circulating neutrophil elastase (NE) and neutrophil volume distribution width (NDW) as neutrophil activation markers and circulating soluble CD163 (sCD163) and monocyte volume distribution width (MDW) as monocyte activation markers in 168 patients suspected of having DIC. The sCD163 provided significant diagnostic value. The prognostic value of sCD163 was comparable to that of D-dimer, but was dependent on other coagulation markers...
May 2011: Thrombosis Research
Robert Bagdasaryan, Zhongren Zhou, Bethany Tierno, Dara Rosenman, Dongsheng Xu
A reliable and cost-effective laboratory method for diagnosing early bacterial infection is needed. The purpose of this study is to compare the sensitivity and specificity of the mean neutrophil volume (MNV) and neutrophil volume distribution width (NDW) parameters with manual band counts, as well as absolute neutrophil count (ANC) and Creactive protein (CRP). We analyzed the clinical history and laboratory data from 242 adult patients with subsequent randomization into 3 groups: patients with no apparent clinical evidence of infection (group 1), localized infection (group 2), and severe infection (group 3)...
2007: Laboratory Hematology: Official Publication of the International Society for Laboratory Hematology
Fernando Chaves, Bethany Tierno, Dongsheng Xu
CONTEXT: The number of band forms and immature neutrophils increases during acute bacterial infection. However, the determination of band counts and other neutrophil morphologic changes, such as the presence of toxic granulation, toxic vacuolization, and Dohle bodies in the cytoplasm, is labor intensive and time consuming, as it requires manual examination by an experienced medical technologist. OBJECTIVE: To investigate the value of the neutrophil volume distribution width (NDW), generated by VCS technology of the Coulter LH 750 hematology analyzer, as an additional predictor of acute infection...
March 2006: Archives of Pathology & Laboratory Medicine
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