Steven L Raymond, Brittany J Mathias, Tyler J Murphy, Jaimar C Rincon, María Cecilia López, Ricardo Ungaro, Felix Ellett, Julianne Jorgensen, James L Wynn, Henry V Baker, Lyle L Moldawer, Daniel Irimia, Shawn D Larson
Neutrophils play a crucial role in combating life-threatening bacterial infections in neonates. Previous studies investigating neonatal cell function have been limited because of restricted volume sampling. Here, using novel microfluidic approaches, we provide the first description of neutrophil chemotaxis and transcriptomics from whole blood of human term and preterm neonates, as well as young adults. Ex vivo percent cell migration, neutrophil velocity, and directionality to N-formylmethionyl-leucyl-phenylalanine were measured from whole blood using time-lapse imaging of microfluidic chemotaxis...
December 2017: Translational Research: the Journal of Laboratory and Clinical Medicine