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mycoplasma pneumoniae

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https://www.readbyqxmd.com/read/28520818/evaluation-of-a-real-time-method-of-simultaneous-amplification-and-testing-in-diagnosis-of-mycoplasma-pneumoniae-infection-in-children-with-pneumonia
#1
Wei Li, You-Hong Fang, Hong-Qiang Shen, De-Hua Yang, Qiang Shu, Shi-Qiang Shang
Mycoplasma pneumoniae (M. pneumoniae) infection can cause community acquired pneumonia in children. A real-time method of simultaneous amplification and testing of M. pneumoniae (SAT-MP) was developed to diagnose M. pneumoniae targeting a region of the ribosomal RNA. The SAT-MP assay can accurately identify M. pneumoniae with a detection range from 101 to 107 CFU/ml. In this study, the specimens from 315 children with pneumonia were collected and analyzed by SAT-MP in parallel with real-time PCR method and IgM ELISA assay...
2017: PloS One
https://www.readbyqxmd.com/read/28515564/delayed-stevens-johnson-syndrome-secondary-to-the-use-of-lamotrigine-in-bipolar-mood-disorder
#2
Kunal Kishor Jha, Durgesh Prasad Chaudhary, Tshristi Rijal, Semanta Dahal
Lamotrigine is a mood-stabilizing drug used in maintenance treatment of bipolar I disease. There are adverse effects with lamotrigine such as a headache, blurred vision, diplopia, somnolence, ataxia, dizziness, rash, Stevens-Johnson syndrome (SJS), and toxic epidermal necrolysis. SJS is a life-threatening, blistering mucocutaneous disease. SJS is characterized by the presence of flat, diffuse erythematous maculopapular rashes with the involvement of <10% of the body surface area. Standard trigger is drugs including anticonvulsants, antibiotics, and Mycoplasma pneumoniae infection...
March 2017: Indian Journal of Psychological Medicine
https://www.readbyqxmd.com/read/28513419/histological-findings-in-severe-mycoplasma-pneumoniae-pneumonia
#3
Naoyuki Miyashita, Mitsuo Narita, Takaaki Tanaka, Hiroto Akaike, Hideto Teranishi, Tomohiro Oishi, Takashi Nakano, Kazunobu Ouchi, Niro Okimoto
No abstract text is available yet for this article.
May 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28497921/macrolide-resistance-and-its-impacts-on-m-pneumoniae-pneumonia-in-children-comparison-of-two-recent-epidemics-in-korea
#4
Jong Hyun Kim, Jee Yong Kim, Chang Hoon Yoo, Won Hee Seo, Young Yoo, Dae Jin Song, Ji Tae Choung
PURPOSE: The aim of this study was to investigate the change in macrolide resistance rate in pediatric Mycoplasma pneumoniae pneumonia and to evaluate the influence of macrolide-resistant M. pneumoniae (MRMP) on the clinical course of disease, by comparing 2 recent, consecutive epidemics in Korea. METHODS: A total of 250 patients with M. pneumoniae pneumonia admitted to a single tertiary hospital were enrolled in this study. Detection of MRMP was based on specific point mutations in domain V of the 23S rRNA gene...
July 2017: Allergy, Asthma & Immunology Research
https://www.readbyqxmd.com/read/28497596/development-of-immunochromatographic-colloidal-gold-test-strip-for-rapid-detection-of-haemophilus-influenzae-in-clinical-specimens
#5
MengMeng Kong, Bo Yang, ChunJie Gong, Hui Wang, Xin Li, Kesheng Zhao, Jiaji Li, Feng Wu, Xiao Liu, Zheng Hu
AIMS: A simple and fast test strip for simultaneous detection of Haemophilus influenzae, based on the theory of colloidal gold immunochromatography was developed. METHODS AND RESULTS: In this study, monodispersional colloidal gold and gold-labeled polyclonal antibody (AbP6Line) specific to the linear antigenic determinant OMP6 of H. influenzae were prepared, and identified by UV-Visible spectroscopy (200-700nm) and transmission electron microscopy (TEM). The test strip was assembled on a plastic backing containing a sample pad, a conjugate pad, an absorbent pad and a nitrocellulose membrane (NC), on which test and control lines were sticked...
May 12, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28495779/complete-genome-sequence-of-mycoplasma-bovis-strain-08m
#6
Shengli Chen, Huafang Hao, Ping Zhao, Pengcheng Gao, Ying He, Wenheng Ji, Zhanhui Wang, Zhongxin Lu, Yongsheng Liu, Yuefeng Chu
Mycoplasma bovis is a major bacterial pathogen that can cause respiratory disease, mastitis, and arthritis in cattle. We report here the complete and annotated genome sequence of M. bovis strain 08M, isolated from a calf lung with pneumonia in China.
May 11, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28494930/a-ros-dependent-and-caspase-3-mediated-apoptosis-in-sheep-bronchial-epithelial-cells-in-response-to-mycoplasma-ovipneumoniae-infections
#7
Di Xue, Yanan Li, Zhongjia Jiang, Guangcun Deng, Min Li, Xiaoming Liu, Yujiong Wang
Mycoplasma Ovipneumoniae (M. ovipneumoniae) is a primary etiological agent of enzootic pneumonia in sheep and goats. It can enter and colonize ovine respiratory epithelial cells to establish an infection, which leads a serious cell death of epithelial cells. However, the nature of the interaction between pathogen of M. ovipneumoniae and host cells in the cell injury is currently not well understood. In this study, we investigated the epithelial cell apoptosis caused by an infection of M. ovipneumoniae in sheep primary air-liquid interface (ALI) epithelial cultures...
May 2017: Veterinary Immunology and Immunopathology
https://www.readbyqxmd.com/read/28492327/versatile-methodology-for-glycosurfaces-direct-ligation-of-nonderivatized-reducing-saccharides-to-poly-pentafluorophenyl-acrylate-grafted-surfaces-via-hydrazide-conjugation
#8
Li Chen, Deborah Leman, Caitlin R Williams, Karson Brooks, Duncan C Krause, Jason Locklin
In this work, we report a convenient and versatile strategy for surface-grafted glycopolymer constructs with the goal of surface modification that controls the chemical presentation and grafting density of carbohydrate side chains. This approach employs a difunctional hydrazine linker, chemically modified to an active ester containing poly(pentafluorophenyl acrylate) grafted scaffold, to conjugate a variety of saccharides through the reducing end. The successive conjugation steps are carried out under mild conditions and yield high surface densities of sugars, as high as 4...
May 18, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28491249/the-seroprevalence-of-mycoplasma-pneumoniae-igm-and-igg-antibodies-in-patients-with-ischemic-stroke
#9
Maryam Roham, Khatereh Anbari, Samira Mirhabibi, Gholamreza Goudarzi
BACKGROUND AND OBJECTIVES: Association between Mycoplasma pneumoniae infection and increased risk for brain stroke has been well understood. Hence, the value of serologic tests for assessing causative relationship between this infection and brain stroke seems to be high. The present study aimed to determine serum level of anti-Mycoplasma pneumoniae antibodies in patients with brain stroke and to compare it with non-stroke patients. MATERIALS AND METHODS: This cross-sectional study was performed on 97 consecutive ischemic stroke patients and 97 sex and age-matched non-stroke patients...
December 2016: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/28490485/molecular-characterization-of-mycoplasma-pneumoniae-infections-in-two-rural-populations-of-thailand-from-2009-2012
#10
Toni Whistler, Pongpun Sawatwong, Maureen H Diaz, Alvaro J Benitez, Bernard J Wolff, Patranuch Sapchookul, Somsak Thamthitiwat, Jonas M Winchell
Studies on Mycoplasma pneumoniae in Thailand have focused on urban centers and have not included the molecular characterization. In an attempt to provide a more comprehensive understanding of this organism, we conducted a systematic random sampling to identify 3000 nasopharyngeal swab specimens, collected from January 2009 through July 2012 during population-based surveillance for influenza-like illness in two rural provinces. M. pneumoniae was detected by real-time PCR in 175 (5.8%) specimens. Genotyping was performed using the major adhesion protein (P1) and multilocus variable-number tandem-repeat analysis (MLVA)...
May 10, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28480285/influence-of-antibiotics-on-the-detection-of-bacteria-by-culture-based-and-culture-independent-diagnostic-tests-in-patients-hospitalized-with-community-acquired-pneumonia
#11
Aaron M Harris, Anna M Bramley, Seema Jain, Sandra R Arnold, Krow Ampofo, Wesley H Self, Derek J Williams, Evan J Anderson, Carlos G Grijalva, Jonathan A McCullers, Andrew T Pavia, Richard G Wunderink, Kathryn M Edwards, Jonas M Winchell, Lauri A Hicks
BACKGROUND: Specimens collected after antibiotic exposure may reduce culture-based bacterial detections. The impact on culture-independent diagnostic tests is unclear. We assessed the effect of antibiotic exposure on both of these test results among patients hospitalized with community-acquired pneumonia (CAP). METHODS: Culture-based bacterial testing included blood cultures and high-quality sputum or endotracheal tube (ET) aspirates; culture-independent testing included urinary antigen testing (adults) for Streptococcus pneumoniae and Legionella pneumophila and polymerase chain reaction (PCR) on nasopharyngeal and oropharyngeal (NP/OP) swabs for Mycoplasma pneumoniae and Chlamydia pneumoniae...
2017: Open Forum Infectious Diseases
https://www.readbyqxmd.com/read/28475737/evolution-of-acute-infection-with-atypical-bacteria-in-a-prospective-cohort-of-children-with-community-acquired-pneumonia-receiving-amoxicillin
#12
Cristiana M Nascimento-Carvalho, Gabriel Xavier-Souza, Ana-Luisa Vilas-Boas, Maria-Socorro H Fontoura, Aldina Barral, Mirja Puolakkainen, Olli Ruuskanen
Background: Atypical bacteria are treatable causative agents of community-acquired pneumonia (CAP). However, there is no conclusive evidence that a child with CAP should receive empirical treatment against such agents. Objectives: We assessed the possibility of association between clinical failure and acute infection by these bacteria among children with CAP treated with amoxicillin. Patients and methods: Patients aged 2-59 months with non-severe CAP received amoxicillin during prospective follow-up...
May 5, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28471864/mycoplasma-pneumoniae-a-cross-sectional-population-based-comparison-of-disease-severity-in-pre-school-and-school-age-children
#13
Christopher Stephen Inchley, Are Stuwitz Berg, Afsaneh Vahdani Benam, Anne Katrine Kvissel, Truls Michael Leegaard, Britt Nakstad
BACKGROUND: Mycoplasma pneumoniae (MP) causes epidemics of upper respiratory disease and pneumonia. It is thought that MP usually causes milder upper respiratory disease in pre-school children, with a greater chance of pneumonia in school-age children. In this population-based cross-sectional study, we present evidence that severe MP infection is more common in pre-school children than previously thought. METHODS: During an MP epidemic in our area widespread health service and public awareness lead to extensive testing for MP...
May 3, 2017: Pediatric Infectious Disease Journal
https://www.readbyqxmd.com/read/28458673/inflammatory-responses-regulating-alveolar-ion-transport-during-pulmonary-infections
#14
REVIEW
Christin Peteranderl, Jacob I Sznajder, Susanne Herold, Emilia Lecuona
The respiratory epithelium is lined by a tightly balanced fluid layer that allows normal O2 and CO2 exchange and maintains surface tension and host defense. To maintain alveolar fluid homeostasis, both the integrity of the alveolar-capillary barrier and the expression of epithelial ion channels and pumps are necessary to establish a vectorial ion gradient. However, during pulmonary infection, auto- and/or paracrine-acting mediators induce pathophysiological changes of the alveolar-capillary barrier, altered expression of epithelial Na,K-ATPase and of epithelial ion channels including epithelial sodium channel and cystic fibrosis membrane conductance regulator, leading to the accumulation of edema and impaired alveolar fluid clearance...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28440917/atf3-inhibits-the-inflammation-induced-by-mycoplasma-pneumonia-in-vitro-and-in-vivo
#15
Jing Wang, Wei Cheng, Zhen Wang, Lihong Xin, Wen Zhang
OBJECTIVES: Activating transcription factor-3 (ATF3) is a key regulator of inflammatory responses. We aimed to investigate the effects and mechanisms of ATF3 on the inflammatory cytokines are induced by Mycoplasma pneumonia (MP). STUDY DESIGN: RAW264.7 and mouse peritoneal macrophages were exposed to various time with or without MP infection (3, 6, 12, 24, and 48 h), and detect the expression of ATF3. Adenovirus-expression of ATF3 (Ad/ATF3) or Ad/βgal was transfected into cells which were exposed to MP for 48 h, RT-PCR and ELISA was used to evaluate the expression and secretion of TNF-α, IL-1β, IL-6, and IL-18...
April 25, 2017: Pediatric Pulmonology
https://www.readbyqxmd.com/read/28431520/infectious-agents-is-a-risk-factor-for-myxomatous-mitral-valve-degeneration-a-case-control-study
#16
Marcos Gradim Tiveron, Pablo Maria Alberto Pomerantzeff, Maria de Lourdes Higuchi, Marcia Martins Reis, Jaqueline de Jesus Pereira, Joyce Tieko Kawakami, Renata Nishiyama Ikegami, Carlos Manuel de Almeida Brandao, Fabio Biscegli Jatene
BACKGROUND: The etiology of myxomatous mitral valve degeneration (MVD) is not fully understood and may depend on time or environmental factors for which the interaction of infectious agents has not been documented. The purpose of the study is to analyze the effect of Mycoplasma pneumoniae (Mp), Chlamydophila pneumoniae (Cp) and Borrelia burgdorferi (Bb) on myxomatous mitral valve degeneration pathogenesis and establish whether increased in inflammation and collagen degradation in myxomatous mitral valve degeneration etiopathogenesis...
April 21, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28430965/nrf2-regulates-the-inflammatory-response-including-heme-oxygenase-1-induction-by-mycoplasma-pneumoniae-lipid-associated-membrane-proteins-in-thp-1-cells
#17
Jihong Hu, Chunyan Chen, Guangli Ou, Xiaoxing You, Tianping Tan, Xinnian Hu, Yihua Zeng, Minjun Yu, Cuiming Zhu
A series of inflammatory responses caused by Mycoplasma pneumoniae largely depend on the lipid-associated membrane proteins (LAMPs). Nuclear factor E2-related factor 2 (Nrf2), a transcription factor, is considered to be a critical modulator of inflammatory responses and cellular redox homeostasis. Monocytes play an important role in the invasion and immunity to resist pathogens. Here, we investigated the role of Nrf2 in the anti-inflammatory response stimulated by LAMPs using the human monocyte cell line THP-1...
June 1, 2017: Pathogens and Disease
https://www.readbyqxmd.com/read/28413775/an-interesting-case-of-mycoplasma-pneumonia-associated-multisystem-involvement-and-diffuse-alveolar-hemorrhage
#18
Rashmi Mishra, Edison Cano, Sindhaghatta Venkatram, Gilda Diaz-Fuentes
Severe mycoplasma pneumonia is a rare entity with only 0.5-2% of cases having a fulminant course. We present a 74-year-old woman with hypertension, diabetes mellitus and remote history of marginal zone B-cell lymphoma admitted with abdominal pain and diarrhea of 1-2 days associated with body-aches, dyspnea, dry cough and weight loss for 2-3 weeks. On physical exam, she was febrile, tachypneic, tachycardic and hypoxic on room air. Chest examination revealed diffuse crackles and end-expiratory wheezes. Laboratory tests showed anemia, acute-on-chronic kidney injury and hyaline casts and epithelial cells in the urine analysis...
2017: Respiratory Medicine Case Reports
https://www.readbyqxmd.com/read/28410368/comprehensive-bioinformatics-analysis-of-mycoplasma-pneumoniae-genomes-to-investigate-underlying-population-structure-and-type-specific-determinants
#19
Maureen H Diaz, Heta P Desai, Shatavia S Morrison, Alvaro J Benitez, Bernard J Wolff, Jason Caravas, Timothy D Read, Deborah Dean, Jonas M Winchell
Mycoplasma pneumoniae is a significant cause of respiratory illness worldwide. Despite a minimal and highly conserved genome, genetic diversity within the species may impact disease. We performed whole genome sequencing (WGS) analysis of 107 M. pneumoniae isolates, including 67 newly sequenced using the Pacific BioSciences RS II and/or Illumina MiSeq sequencing platforms. Comparative genomic analysis of 107 genomes revealed >3,000 single nucleotide polymorphisms (SNPs) in total, including 520 type-specific SNPs...
2017: PloS One
https://www.readbyqxmd.com/read/28404679/evaluation-of-taqman-array-card-tac-for-the-detection-of-central-nervous-system-infections-in-kenya
#20
Clayton O Onyango, Vladimir Loparev, Shirley Lidechi, Vinod Bhullar, D Scott Schmid, Kay Radford, Michael K Lo, Paul Rota, Barbara W Johnson, Jorge Munoz, Martina Oneko, Deron Burton, Carolyn M Black, John Neatherlin, Joel M Montgomery, Barry Fields
Infections of the central nervous system (CNS) are often acute with significant morbidity and mortality. Routine diagnosis of such infections is limited in developing countries and requires modern equipment in advanced laboratories that may be unavailable to a number of patients in sub-Saharan Africa. We developed a TaqMan Array Card (TAC) that detects multiple pathogens simultaneously from cerebrospinal fluid (CSF). The 21-pathogen TAC assays include two parasites (Balamuthia mandrillaris and Acanthamoeba), six bacterial pathogens (Streptococcus pneumoniae, Haemophilus influenzae, Neisseria meningitidis, Mycoplasma pneumoniae, Mycobacterium tuberculosis, and Bartonella) and 13 viruses (parechovirus, dengue, nipah, varicella zoster, mumps, measles, lyssa, herpes simplex virus 1 and 2, Epstein Barr virus, enterovirus, cytomegalovirus and chikungunya)...
April 12, 2017: Journal of Clinical Microbiology
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