Novel N Chegou, Paulin N Essone, Andre G Loxton, Kim Stanley, Gillian F Black, Gian D van der Spuy, Paul D van Helden, Kees L Franken, Shreemanta K Parida, Michel R Klein, Stefan H E Kaufmann, Tom H M Ottenhoff, Gerhard Walzl
BACKGROUND: Recent interferon gamma (IFN-γ)-based studies have identified novel Mycobacterium tuberculosis (M.tb) infection phase-dependent antigens as diagnostic candidates. In this study, the levels of 11 host markers other than IFN-γ, were evaluated in whole blood culture supernatants after stimulation with M.tb infection phase-dependent antigens, for the diagnosis of TB disease. METHODOLOGY AND PRINCIPAL FINDINGS: Five M.tb infection phase-dependent antigens, comprising of three DosR-regulon-encoded proteins (Rv2032, Rv0081, Rv1737c), and two resucitation promoting factors (Rv0867c and Rv2389c), were evaluated in a case-control study with 15 pulmonary TB patients and 15 household contacts that were recruited from a high TB incidence setting in Cape Town, South Africa...
2012: PloS One