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fetuin a

Mariola A Dietrich, Piotr Hliwa, Mikołaj Adamek, Dieter Steinhagen, Halina Karol, Andrzej Ciereszko
The environmental temperature affects plasma biochemical indicators, antioxidant status and hematological and immunological parameters in fish. So far, only single blood proteins have been identified in response to temperature changes. The aim of this study was to compare the proteome of carp blood plasma from males acclimated to warm (30 °C) and cold (10 °C) temperatures by two-dimensional differential gel electrophoresis followed by MALDI-TOF/TOF mass spectrometry. A total of 47 spots were found to be differentially regulated by temperature (>1...
March 12, 2018: Fish & Shellfish Immunology
Janine Kröger, Karina Meidtner, Norbert Stefan, Marcela Guevara, Nicola D Kerrison, Eva Ardanaz, Dagfinn Aune, Heiner Boeing, Miren Dorronsoro, Courtney Dow, Guy Fagherazzi, Paul W Franks, Heinz Freisling, Marc J Gunter, José María Huerta, Rudolf Kaaks, Timothy J Key, Kay Tee Khaw, Vittorio Krogh, Tilman Kühn, Francesca Romana Mancini, Amalia Mattiello, Peter M Nilsson, Anja Olsen, Kim Overvad, Domenico Palli, J Ramón Quirós, Olov Rolandsson, Carlotta Sacerdote, Núria Sala, Elena Salamanca-Fernández, Ivonne Sluijs, Annemieke Mw Spijkerman, Anne Tjonneland, Konstantinos K Tsilidis, Rosario Tumino, Yvonne T van der Schouw, Nita G Forouhi, Stephen J Sharp, Claudia Langenberg, Elio Riboli, Matthias B Schulze, Nicholas J Wareham
Fetuin-A, a hepatic-origin protein, is strongly positively associated with risk of type 2 diabetes in human observational studies, but it is unknown whether this association is causal. We aimed to study the potential causal relation of circulating fetuin-A to risk of type 2 diabetes in a Mendelian Randomization study with SNPs located in the fetuin-A-encoding AHSG gene. We used data from eight European countries of the prospective EPIC-InterAct case-cohort study including 10,020 incident cases. Plasma fetuin-A concentration was measured in a subset of 965 subcohort participants and 654 cases...
March 9, 2018: Diabetes
Jihua Wu, Zhiwei Tao, Yaoliang Deng, Quan Liu, Yunlong Liu, Xiaofeng Guan, Xiang Wang
Calcifying nanoparticles (CNPs) play an important role in kidney stone formation, but the mechanism(s) are unclear. CNPs were isolated and cultured from midstream urine of patients with kidney stones. CNP morphology and characteristics were examined by electron microscopy and electrophoresis analysis. Chemical composition was analyzed using energy-dispersive X-ray microanalysis and Western blotting. Human renal proximal convoluted tubule cell (HK-2) cultures were exposed to CNPs for 0, 12 and 72 h, and production of reactive oxygen species (ROS), mitochondrial membrane potential and apoptosis levels were evaluated...
March 6, 2018: Urolithiasis
Zhi Yu, Sylvia H Ley, Qi Sun, Frank B Hu, Vasanti S Malik
Few studies have evaluated the relationships between intake of sugar-sweetened beverages (SSB) and intermediate biomarkers of cardiometabolic risk. Associations between artificially sweetened beverages (ASB) and fruit juice with cardiometabolic biomarkers are also unclear. We investigated habitual SSB, ASB and fruit juice intake in relation to biomarkers of hepatic function, lipid metabolism, inflammation and glucose metabolism. We analysed cross-sectional data from 8492 participants in the Nurses' Health Study who were free of diabetes and CVD...
March 2018: British Journal of Nutrition
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No abstract text is available yet for this article.
January 2018: Medicine (Baltimore)
Jiejie Zhao, Xuelian Xiong, Yao Li, Xing Liu, Tao Wang, Hong Zhang, Yang Jiao, Jingjing Jiang, Huijie Zhang, Qiqun Tang, Xin Gao, Xuejun Li, Yan Lu, Bin Liu, Cheng Hu, Xiaoying Li
Type 2 diabetes has become one of the most serious and long-term threats to human health. However, the molecular mechanism that links obesity to insulin resistance remains largely unknown. Here, we show that F-box and WD repeat domain-containing 7 (FBXW7), an E3 ubiquitin-protein ligase, is markedly downregulated in the liver of two obese mouse models and obese human subjects. We further identify a functional low-frequency human FBXW7 coding variant (p. Ala204Thr) in Chinese population, which is associated with elevated blood glucose and type 2 diabetes risk...
February 23, 2018: Diabetes
Fabio Nascimbeni, Dante Romagnoli, Stefano Ballestri, Enrica Baldelli, Simonetta Lugari, Valentina Sirotti, Valentina Giampaoli, Amedeo Lonardo
BACKGROUND AND AIMS: Nonalcoholic fatty liver disease (NAFLD) is strongly associated with both atherosclerotic cardiovascular disease (CVD) and Fetuin-A. However, the association of Fetuin-A with atherosclerosis is more controversial. We hypothesized that the pathogenic interplay of NAFLD, Fetuin-A and atherosclerosis varies based on arterial site. Accordingly, we aimed to assess NAFLD prevalence, Fetuin-A values and their relationship with symptomatic atherosclerosis occurring in different localizations: coronary artery disease (CAD) vs...
February 16, 2018: Diseases (Basel)
Maher Sayhi, Oussama Ouerghi, Kamel Belgacem, Marwa Arbi, Yudum Tepeli, Abdeljalil Ghram, Ülkü Anik, Lars Österlund, Dhafer Laouini, Mohamed Fethi Diouani
Influenza is a viral infectious disease considered as a source of many health problems and enormous socioeconomic disruptions. Conventional methods are inadequate for in-field detection of the virus and generally suffer from being laborious and time-consuming. Thus, studies aiming to develop effective alternatives to conventional methods are urgently needed. In this work, we developed an approach for the isolation and detection of influenza A virus subtype H9N2. For this aim, two specific influenza receptors were used...
February 7, 2018: Biosensors & Bioelectronics
Dae-Won Lee, Hira Fatima, Kyo-Seon Kim
Magnetic nanoparticles have been intensively developed and applied in several biomedical applications such as targeted delivery, drug therapy, hyperthermia, magnetic resonance imaging and bioseparation etc. This work describes a simple and convenient method to synthesize silica coated Fe3O4 nanoparticles which have the potential to be used in the bioseparation of fetuin from fetal bovine serum and albumin from the egg. In this work, uniform Fe3O4 particles were prepared through the one-pot solvothermal process at 200 °C for 12 h using a sole iron precursor (FeCl3 · 6H2O) and then were coated with SiO2 to prepare silica coated Fe3O4 nanoparticles by hydrolysis and condensation of tetraethyl orthosilicate in ethanol and H2O medium...
February 1, 2018: Journal of Nanoscience and Nanotechnology
Nada Dimkovic, Georg Schlieper, Aleksandar Jankovic, Zivka Djuric, Marcus Ketteler, Tatjana Damjanovic, Petar Djuric, Jelena Marinkovic, Zoran Radojcic, Natasa Markovic, Jürgen Floege
PURPOSE: Cardiovascular calcifications (CVC) are present in up to 70% of non-diabetic dialysis patients. Sparse data are available on predictors of very long-term outcomes of such patients. The Belgrade Aachen Study on Calcification in Hemodialysis patients (BASCH study) aimed to study this using a comprehensive CVC assessment. METHODS: We prospectively analyzed 220 hemodialysis patients followed for a mean of 76 months (median 73 months, range 6-160 months). We compared patients deceased from cardiovascular diseases (CVD) and survivors...
February 13, 2018: International Urology and Nephrology
Chaterina Sujana, Cornelia Huth, Astrid Zierer, Sophie Meesters, Julie Sudduth-Klinger, Wolfgang Koenig, Christian Herder, Annette Peters, Barbara Thorand
Objective We investigated the association of circulating fetuin-A with incident T2D particularly examining potential sex differences. Additionally, we determined whether putative associations were independent of subclinical inflammation, adiponectin and liver fat content. Design Case-cohort study plus systematic meta-analysis. Methods We investigated the association between baseline fetuin-A levels and incident T2D in the MONICA/KORA Augsburg study using Cox proportional hazards analyses. Furthermore, we conducted a systematic review within PUBMED and EMBASE and pooled association estimates of eligible studies with the MONICA/KORA Augsburg data using a DerSimonian-Laird random effects model...
February 8, 2018: European Journal of Endocrinology
Takaaki Yamamoto, Hideyo Ugai, Haruyuki Nakayama-Imaohji, Ayano Tada, Miad Elahi, Hitoshi Houchi, Tomomi Kuwahara
The human gut commensal Bacteroides fragilis produces sialidases that remove a terminal sialic acid from host-derived polysaccharides. Sialidase is considered to be involved in B. fragilis infection pathology. A native B. fragilis sialidase has been purified and characterized, and was shown to be post-translationally modified by glycosylation. However, the biochemical properties of recombinant B. fragilis sialidase expressed in a heterologous host remain uncharacterized. In this study, we examined the enzymatic properties of the 60-kDa sialidase NanH1 of B...
February 9, 2018: Anaerobe
Noemi Procopio, Anna Williams, Andrew T Chamberlain, Michael Buckley
Current methods for the evaluation of post-mortem interval (PMI) of skeletal remains suffer from poor accuracy, due to the great number of variables that affect the diagenetic process and to the lack of specific guidelines to address this issue. During decomposition, proteins can undergo cumulative decay over the time, resulting in a decrease in the range and abundance of proteins present (i.e., the proteome) in different tissues as well as in an increase of post-translational modifications occurring on these proteins...
January 30, 2018: Journal of Proteomics
Zhibin Li, Changqin Liu, Xiulin Shi, Zheng Chen, Dongmei Wang, Long Li, Yichang Tu, Mingzhu Lin, Suhuan Liu, Shuyu Yang, Xuejun Li
Elevated serum fetuin-B is suggested to be associated with insulin resistance, but it is unknown if this association is causal. The aim of this study was to explore the potential causal relationship between fetuin-B and insulin resistance.We used Mendelian randomization analysis by incorporating information of genetic variants in FETUB and serum fetuin-B concentrations with insulin resistance in 1148 obese Chinese adults.Common genetic variants (FETUB rs4686434, rs6785067, and rs3733159) were significantly associated with serum fetuin-B concentrations but not with insulin resistance...
December 2017: Medicine (Baltimore)
Noemi Procopio, Andrew T Chamberlain, Michael Buckley
Proteomic analyses are becoming more widely used in archaeology, particularly for sample characterisation, including species identification, with recent indications of its potential assistance in estimating biological and geological age. However, it remains unclear to what extent different burial environments impact upon aspects of proteome decay. Teeth have to date been much less studied than bone but are ideal to explore decay due to the negligible turnover that occurs in dentine. Here we investigated the proteome variability and deamidation levels of different sections of molar teeth from archaeological bovine mandibles as well as their mandibular bone...
January 22, 2018: Journal of Proteome Research
Ishwarlal Jialal, Sridevi Devaraj
Metabolic syndrome (MetS) is a common global problem that comprises the cardio-metabolic cluster and predisposes to both diabetes and cardiovascular diseases. Although the pathogenic mechanisms have not been elucidated, both increased inflammation and insulin resistance play a pivotal role. It appears that both monocyte/macrophages and adipose tissue (AT) conspire to accentuate both the pro-inflammatory state and increased insulin resistance. Whilst there are scant data on visceral adipose tissue (VAT) and epicardial adipose tissue (EAT) biology, there are data on subcutaneous adipose tissue (SAT) dysregulation...
January 20, 2018: Hormone Molecular Biology and Clinical Investigation
Yutaka Miura, Yoshitaka Iwazu, Kazuhiro Shiizaki, Tetsu Akimoto, Kazuhiko Kotani, Masahiko Kurabayashi, Hiroshi Kurosu, Makoto Kuro-O
Calciprotein particles (CPP) are solid-phase calcium-phosphate bound to serum protein fetuin-A and dispersed as colloids in the blood. Recent clinical studies indicated that serum CPP levels were increased with decline of renal function and associated with inflammation and vascular calcification. However, CPP assays used in these studies measured only a part of CPP over a certain particle size and density. Here we show that such CPP are mostly artifacts generated during processing of serum samples in vitro...
January 19, 2018: Scientific Reports
Linlin Chen, Di Ding, Qianying Sheng, Long Yu, Xiuping Liu, Xinmiao Liang
Glycosylation analysis of proteins from biological sources utilizing mass-spectrometry-based approaches is challenging due to the relatively low abundance of glycopeptides, the structural diversity of glycans as well as the co-existing matrices. In this study, a customized dextran-bonded silica-based stationary phase was introduced for selective enrichment of glycopeptides and glycans from complex biological samples. This material has exhibited superior selectivity and broader glycosylation site coverage over commercial Sepharose in glycoproteomic evaluation...
January 15, 2018: Journal of Separation Science
Yike Wu, Qiuyue Sha, Juan Du, Chang Wang, Liang Zhang, Bi-Feng Liu, Yawei Lin, Xin Liu
Robust, efficient identification and accurate quantification of N-glycans are of great significance in N-glycomics analysis. Here, a simple and rapid derivatization method, based on the combination of microwave-assisted deglycosylation and 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) labeling, was developed for the analysis of N-glycan by high performance liquid chromatography with fluorescence detection (HPLC-FLD). After optimizing various parameters affecting deglycosylation and derivatization by RNase B, the time for N-glycan labeling was shortened to 50 min with ∼10-fold enhancement in detection sensitivity comparing to conventional 2-aminobenzoic acid (2-AA) labeling method...
January 8, 2018: Journal of Chromatography. A
Chang Wang, Yike Wu, Liang Zhang, Bi-Feng Liu, Yawei Lin, Xin Liu
Quantitative analysis of glycans is an emerging field in glycomic research. Herein we present a rapid and effective dual-labeling strategy, in the combination of isotopic derivatization of N-glycosylamine-based glycans by d0/d5-benzoyl chloride and methylamidation of sialic acids, to relatively quantify both neutral and sialylated N-glycans simultaneously by MALDI-MS. The derivatization efficiencies were increased by microwave-accelerated deglycosylation which not only largely reduce the time of glycoprotein deglycosylation but also inhibit the hydrolysis of intermediate glycosylamines produced by PNGase F digestion...
March 9, 2018: Analytica Chimica Acta
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