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https://www.readbyqxmd.com/read/29145098/a-novel-simplified-strategy-of-relative-quantification-n-glycan-quantitative-glycomics-using-electrospray-ionization-mass-spectrometry-through-the-stable-isotopic-labeling-by-transglycosylation-reaction-of-mutant-enzyme-endo-m-n175q
#1
Qing Shi, Ryugo Hashimoto, Tadamune Otsubo, Kiyoshi Ikeda, Kenichiro Todoroki, Hajime Mizuno, Dongri Jin, Toshimasa Toyo'oka, Zhe Jiang, Jun Zhe Min
The lack of a highly sensitive and simple method for the quantitative analysis of glycan has impeded the exploration of protein glycosylation patterns (glycomics), evaluation of antibody drug stability, and screening of disease glycan biomarkers. In this study, we describe a novel and simplified quantitative glycomics strategy. Quantitation by mutant enzyme reaction stable isotope labeling (QMERSIL) to label the N-glycans with either a nondeuterated (d0-) or deuterated (d8-) 4-(2,4-Dinitro-5-piperazin-1-yl-phenyl)-1,1-dimethyl-piperazin-1-ium (MPDPZ)-Boc-asparaginyl-N-acetyl-d-glucosamine (Boc-Asn-GlcNAc) acceptor of a positive charge structure through the glycosynthase (Endo-M-N175Q) transglycosylation reaction with mass spectrometry facilitates comparative glycomics...
November 12, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/29138227/the-hepatokines-fetuin-a-and-fetuin-b-are-up-regulated-in-the-state-of-hepatic-steatosis-and-may-differently-impact-on-glucose-homeostasis-in-humans
#2
Andreas Peter, Marketa Kovarova, Harald Staiger, Jürgen Machann, Fritz Schick, Alfred Königsrainer, Ingmar Koenigsrainer, Erwin D Schleicher, Andreas Fritsche, Hans-Ulrich Häring, Norbert Stefan
The liver is a central regulator of whole-body glucose and lipid homeostasis and hepatokines, like fetuin-A, have been identified as markers and mediators of fatty liver-induced cardiometabolic risk. The closely related protein fetuin-B was shown to be up-regulated in the fatty liver and to impact on glucose homeostasis in mice. In the present study we aimed to test the relevance of these findings in humans. In 55 subjects hepatic mRNA expression of both hepatokines, fetuin-A and fetuin-B, associated positively with liver triglyceride content, while only fetuin-A expression associated with the homeostatic model assessment of insulin resistance...
November 14, 2017: American Journal of Physiology. Endocrinology and Metabolism
https://www.readbyqxmd.com/read/29134617/vascular-calcification-is-not-related-to-serum-fetuin-a-and-osteopontin-levels-in-hemodialysis-patients
#3
O Ulutas, M C Taskapan, A Dogan, T Baysal, H Taskapan
INTRODUCTION: Vascular calcification (VC) in hemodialysis (HD) patients is a sign of severe cardiovascular disease and can predict cardiovascular outcomes. Fetuin-A and osteopontin (OPN) inhibit VC. Serum fetuin-A levels are lower in patients with end-stage kidney disease (ESKD) and in those who are on chronic HD therapy. However, there are limited data concerning OPN in patients who are on dialysis. The aim of our study was to determine VC in HD patients, the relationship between VC and 25-OH-vitamin D, fetuin-A, and OPN levels, and independent predictors of VC...
November 13, 2017: International Urology and Nephrology
https://www.readbyqxmd.com/read/29134205/towards-the-electrochemical-diagnostic-of-influenza-virus-development-of-a-graphene-au-hybrid-nanocomposite-modified-influenza-virus-biosensor-based-on-neuraminidase-activity
#4
Ülkü Anik, Yudum Tepeli, Maher Sayhi, Jihene Nsiri, Mohamed Fethi Diouani
An effective electrochemical influenza A biosensor based on a graphene-gold (Au) hybrid nanocomposite modified Au-screen printed electrode has been developed. The working principle of the developed biosensor relies on the measurement of neuraminidase (N) activity. After the optimization of experimental parameters like the effect of bovine serum albumin addition and immobilization times of fetuin A and PNA lectin, the analytical characteristics of the influenza A biosensor were investigated. As a result, a linear range between 10(-8) U mL(-1) and 10(-1) U mL(-1) was found with a relative standard deviation value of 3...
November 14, 2017: Analyst
https://www.readbyqxmd.com/read/29132248/gene-delivery-system-of-pdna-using-the-blood-glycoprotein-fetuin
#5
Yukinobu Kodama, Hiroki Hanamura, Takahiro Muro, Hiroo Nakagawa, Tomoaki Kurosaki, Tadahiro Nakamura, Takashi Kitahara, Shigeru Kawakami, Mikiro Nakashima, Hitoshi Sasaki
Fetuin is a biocompatible plasma protein and strongly enhances phagocytosis of bacteria, DNA, and apoptotic cells by peripheral blood cells such as monocytes, macrophages, and dendritic cells. We developed a novel gene delivery system: ternary complexes constructed with pDNA, polyethylenimine (PEI), and fetuin. Without covalent binding, fetuin was able to coat pDNA-PEI complexes, and stable anionic nanoparticles formed at a weight ratio greater than 30. Optimized pDNA-PEI-fetuin complexes significantly decreased the cytotoxicity of pDNA-PEI complexes in the melanoma cell line B16F10...
November 13, 2017: Journal of Drug Targeting
https://www.readbyqxmd.com/read/29131649/characterization-of-proteomes-extracted-through-collagen-based-stable-isotope-and-radiocarbon-dating-methods
#6
Caroline Wadsworth, Michael Buckley
Isotope analyses on "collagen" extracted from ancient bone have been routinely used for dietary and chronological inferences worldwide for decades. These methods involve the decalcification of biomineralized tissues with acid, often followed by processes to remove exogenous contaminants, and then gelatinization of what is often described as the "collagen" fraction. However, little is known about the relative content of collagen to the many other noncollagenous proteins (NCPs) potentially present. Some of these NCPs have great longevity in ancient bone, and some, for example, fetuin-A, are useful for obtaining better taxonomic information than collagen...
November 13, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/29127490/fetuin-a-levels-and-risk-of-type-2-diabetes-mellitus-a-systematic-review-and-meta-analysis
#7
Vivian Yawei Guo, Bing Cao, Chunyan Cai, Kenneth King-Yip Cheng, Bernard Man Yung Cheung
AIMS: Fetuin-A has been linked to insulin resistance and obesity. Its role in the pathogenesis of type 2 diabetes (T2DM) has also been discussed. We aimed to investigate the prospective association of fetuin-A and the risk of T2DM in a systematic review and meta-analysis. METHODS: A systematic search of studies from the MEDLINE, EMBASE, Pubmed and Web of Science using fetuin-A, diabetes and various synonyms was conducted up to June 5, 2017. Relevant studies were extracted by two reviewers independently...
November 11, 2017: Acta Diabetologica
https://www.readbyqxmd.com/read/29124050/association-between-healthy-eating-index-2010-and-fetuin-a-levels-in-patients-with-type-2-diabetes-a-case-control-study
#8
Farzad Roshanzamir, Maryam Miraghajani, Marjan Mansourian, Reza Ghiasvand, Seyyed Morteza Safavi
The Healthy Eating Index-2010 (HEI-2010) assesses compliance with the 2010 Dietary Guidelines for Americans. Studies suggest that adherence to the HEI-2010 is related to lower the risk of type 2 diabetes (T2D). Fetuin-A, a novel biomarker for T2D, may play a linking role in the inverse association between HEI-2010 and T2D. Thus, a case-control analysis involving 107 patients with T2D and107 healthy subjects was conducted to determine the association between HEI-2010 and serum fetuin-A levels. The results of simple regression analysis showed that fetuin-A levels were positively associated with full name of body mass index (BMI) (p < 0...
October 2017: Clinical Nutrition Research
https://www.readbyqxmd.com/read/29122442/serum-fetuin-a-levels-in-obese-children-with-biopsy-proven-nonalcoholic-fatty-liver-disease
#9
V Pampanini, E Inzaghi, D Germani, A Alterio, A Puglianiello, A Alisi, V Nobili, S Cianfarani
BACKGROUND AND AIMS: Fetuin-A has been proposed as a marker of liver damage in adults with obesity-related NAFLD. The aim of this study was to test serum fetuin-A concentrations in obese children with NAFLD diagnosed either by ultrasonography or by liver biopsy and to determine its applicability as predictive tool in pediatric NAFLD. METHODS AND RESULTS: Metabolic parameters and fetuin-A levels were investigated in 81 obese children with NAFLD diagnosed by biopsy, 79 obese children with NAFLD defined by liver ultrasonography and 23 lean subjects...
October 3, 2017: Nutrition, Metabolism, and Cardiovascular Diseases: NMCD
https://www.readbyqxmd.com/read/29071522/elevated-serum-fetuin-a-levels-are-associated-with-grades-of-retinopathy-in-type-2-diabetic-patients
#10
Ahu Yilmaz, Tolga Yilmaz, Murat Gunay
PURPOSE: Fetuin-A is a physiological inhibitor of insulin receptor tyrosine kinase and thus associated with insulin resistance, metabolic syndrome, and an increased risk for type 2 diabetes mellitus (T2DM). This study aims to investigate the possible relation between the serum fetuin-A levels and the stages of diabetic retinopathy (DR) in patients with T2DM. METHODS: This prospective study included 82 patients with T2DM and 19 age- and gender-matched healthy controls (HCs) (group 1)...
October 25, 2017: International Ophthalmology
https://www.readbyqxmd.com/read/29069609/development-of-a-data-independent-acquisition-mass-spectrometry-workflow-to-enable-glycopeptide-analysis-without-predefined-glycan-compositional-knowledge
#11
Chi-Hung Lin, Christoph Krisp, Nicolle H Packer, Mark P Molloy
Glycoproteomics investigates glycan moieties in a site specific manner to reveal the functional roles of protein glycosylation. Identification of glycopeptides from data-dependent acquisition (DDA) relies on high quality MS/MS spectra of glycopeptide precursors and often requires manual validation to ensure confident assignments. In this study, we investigated pseudo-MRM (MRM-HR) and data-independent acquisition (DIA) as alternative acquisition strategies for glycopeptide analysis. These approaches allow data acquisition over the full MS/MS scan range allowing data re-analysis post-acquisition, without data re-acquisition...
October 22, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/29039171/-correlation-of-fetuin-b-concentrations-in-serum-and-follicular-fluid-with-outcomes-of-in-vitro-fertilization
#12
Xiaorui Hu, Fengyi Mo, Qing Ma, Long Cui, Pingping Lyu, Yinghui Ye
OBJECTIVE: To investigate the relationship between serum/follicular fluid fetuin B levels and outcome of in vitro fertilization (IVF). METHODS: Infertility women (28 with low fertilization rates, 44 with normal fertilization rates) receiving IVF in Women's Hospital of Zhejiang University School of Medicine during June and December 2016 were enrolled in the study. Serum/follicular fluid fetuin B levels were measured with ELISA method. Correlations of serum and follicular fetuin B level with fertilization outcome of IVF were analyzed with Pearson correlation coefficient and receiver operating characteristic (ROC) curve...
May 25, 2017: Zhejiang da Xue Xue Bao. Yi Xue Ban, Journal of Zhejiang University. Medical Sciences
https://www.readbyqxmd.com/read/29032812/matrix-vesicles-induce-calcification-of-recipient-vascular-smooth-muscle-cells-through-multiple-signaling-pathways
#13
Neal X Chen, Kalisha D O'Neill, Sharon M Moe
In patients with chronic kidney and end-stage renal diseases, the major risk factor for progression of arterial calcification is the presence of existing (baseline) calcification. Here, we tested whether calcification of arteries is extended from calcified vascular smooth muscle cells (VSMCs) to adjacent normal cells by matrix vesicle-induced alteration of cell signaling. Matrix vesicles isolated from VSMC of rats with chronic kidney disease were co-cultured with VSMCs from normal littermates. Endocytosis of vesicles by recipient cells was confirmed by confocal microscopy...
October 9, 2017: Kidney International
https://www.readbyqxmd.com/read/29030114/impairment-of-energy-sensors-sirt1-and-ampk-in-lipid-induced-inflamed-adipocyte-is-regulated-by-fetuin-a
#14
Mrittika Chattopadhyay, Sandip Mukherjee, Subhendu K Chatterjee, Dipanjan Chattopadhyay, Snehasis Das, Subeer S Majumdar, Satinath Mukhopadhyay, Sutapa Mukherjee, Samir Bhattarcharya
Although several reports demonstrated that accumulation of excess lipid in adipose tissue produces defects in adipocyte which leads to the disruption of energy homeostasis causing severe metabolic problems, underlying mechanism of this event remains yet unclear. Here we demonstrate that FetuinA (FetA) plays a critical role in the impairment of two metabolic sensors, SIRT1 and AMPK, in inflamed adipocytes of high fat diet (HFD) mice. A linear increase in adipocyte hypertrophy from 10 to 16 week was in tandem with the increase in FetA and that coincided with SIRT1 cleavage and decrease in pAMPK which adversely affects PGC1α activation...
October 10, 2017: Cellular Signalling
https://www.readbyqxmd.com/read/29021652/urine-and-serum-fetuin-a-levels-in-patients-with-urolithiasis
#15
Rajat Arora, Nitin Abrol, B Antonisamy, S Vanitha, J Chandrasingh, Santosh Kumar, Nitin Kekre, Antony Devasia
INTRODUCTION: Fetuin-A is a glycoprotein secreted by liver and has been shown to inhibit extraosseous mineralization. Urolithiasis may be a manifestation in the urinary tract due to fetuin deficiency in urine. The objective of this study was to compare the 24-h urine and serum fetuin-A levels of patients with and without urolithiasis. METHODS: Serum and 24-h urine fetuin-A levels were measured in 41 patients with bilateral, multiple, or recurrent urinary tract calculi (Group A) and 41 matched controls with no calculi (Group B)...
October 2017: Indian Journal of Urology: IJU: Journal of the Urological Society of India
https://www.readbyqxmd.com/read/28942123/a-single-intraperitoneal-injection-of-bovine-fetuin-a-attenuates-bone-resorption-in-a-murine-calvarial-model-of-particle-induced-osteolysis
#16
Heidrun Jablonski, Christina Polan, Christian Wedemeyer, Gero Hilken, Rüdiger Schlepper, Hagen Sjard Bachmann, Florian Grabellus, Marcel Dudda, Marcus Jäger, Max Daniel Kauther
Particle-induced osteolysis, which by definition is an aseptic inflammatory reaction to implant-derived wear debris eventually leading to local bone destruction, remains the major reason for long-term failure of orthopedic endoprostheses. Fetuin-A, a 66kDa glycoprotein with diverse functions, is found to be enriched in bone. Besides being an important inhibitor of ectopic calcification, it has been described to influence the production of mediators of inflammation. Furthermore, a regulatory role in bone metabolism has been assigned...
September 21, 2017: Bone
https://www.readbyqxmd.com/read/28941598/energy-matched-moderate-and-high-intensity-exercise-training-improves-nonalcoholic-fatty-liver-disease-risk-independent-of-changes-in-body-mass-or-abdominal-adiposity-a-randomized-trial
#17
Nathan C Winn, Liu Ying, R Scott Rector, Elizabeth J Parks, Jamal A Ibdah, Jill A Kanaley
BACKGROUND AND PURPOSE: Exercise training is commonly prescribed for individuals diagnosed with nonalcoholic fatty liver disease (NAFLD); however, consensus regarding the volume and intensity of exercise for optimal benefits is lacking. Thus, we determined whether high intensity interval exercise training (HIIT) produced greater reductions in intrahepatic lipid (IHL) content and NAFLD risk factors compared with energy-matched moderate intensity continuous exercise training (MICT) in obese adults with liver steatosis...
September 20, 2017: Metabolism: Clinical and Experimental
https://www.readbyqxmd.com/read/28936738/vascular-calcification-regulation-by-exosomes-in-the-vascular-wall
#18
Marcel Liberman, Luciana Cavalheiro Marti
Vascular calcification is a tightly regulated process that increases during ageing and occurs mainly in patients with diabetes and chronic renal failure. Exosomes are small membrane vesicles that are synthesized in a particular population of endosomes, also called multivesicular bodies, by inside budding into the lumen of the compartment. After fusion of exosome with the plasma membrane, these internal vesicles are secreted. Exosomes have a defined set of membrane and cytosolic proteins. The physiological function of exosomes is still a matter of debate...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/28931474/could-circulating-fetuin-a-be-a-biomarker-of-aortic-valve-stenosis
#19
Alessandro Di Minno, Marco Zanobini, Veronika A Myasoedova, Vincenza Valerio, Paola Songia, Matteo Saccocci, Matteo Nicola Dario Di Minno, Elena Tremoli, Paolo Poggio
BACKGROUND: Aortic valve stenosis (AVS) is a multifactorial-progressive pathological process. In the past decades, many studies have focus their attention on circulating biomarkers able to identify AVS and/or to predict its progression. One of the many biomarkers studied is the fetuin A. The aim of the present meta-analysis was to evaluate the correlation between fetuin A levels and end-stage AVS. METHODS AND RESULTS: A systematic search was performed in three electronic databases (PubMed, Web of Science and Scopus), looking for studies that compared control subjects with AVS patients and that have measured fetuin A circulating levels in both groups...
December 15, 2017: International Journal of Cardiology
https://www.readbyqxmd.com/read/28923552/fetuin-a-in-aortic-stenosis-the-pathophysiology-crystallizes
#20
EDITORIAL
Björn Redfors, Philippe Généreux
No abstract text is available yet for this article.
December 15, 2017: International Journal of Cardiology
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