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https://www.readbyqxmd.com/read/27845364/a-genomic-island-in-vibrio-cholerae-with-vpi-1-site-specific-recombination-characteristics-contains-crispr-cas-and-type-vi-secretion-modules
#1
Maurizio Labbate, Fabini D Orata, Nicola K Petty, Nathasha D Jayatilleke, William L King, Paul C Kirchberger, Chris Allen, Gulay Mann, Ankur Mutreja, Nicholas R Thomson, Yan Boucher, Ian G Charles
Cholera is a devastating diarrhoeal disease caused by certain strains of serogroup O1/O139 Vibrio cholerae. Mobile genetic elements such as genomic islands (GIs) have been pivotal in the evolution of O1/O139 V. cholerae. Perhaps the most important GI involved in cholera disease is the V. cholerae pathogenicity island 1 (VPI-1). This GI contains the toxin-coregulated pilus (TCP) gene cluster that is necessary for colonization of the human intestine as well as being the receptor for infection by the cholera-toxin bearing CTX phage...
November 15, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27787538/injection-pharyngoplasty-with-autologous-fat-as-treatment-for-stress-velopharyngeal-insufficiency-in-brass-and-woodwind-musicians
#2
Mausumi N Syamal, Paul C Bryson
Importance: Stress velopharyngeal insufficiency (SVPI) is an uncommon but often career-threatening condition affecting professional brass and woodwind musicians. Objectives: To review the evaluation of and treatment for SVPI in professional musicians with lipoinjection to the posterior pharyngeal wall. Design, Setting, and Participants: A retrospective medical record and literature review. Two professional musicians with SVPI treated with autologous lipoinjection to the posterior pharyngeal wall were included...
October 27, 2016: JAMA Otolaryngology—Head & Neck Surgery
https://www.readbyqxmd.com/read/27786291/non-toxigenic-environmental-vibrio-cholerae-o1-strain-from-haiti-provides-evidence-of-pre-pandemic-cholera-in-hispaniola
#3
Taj Azarian, Afsar Ali, Judith A Johnson, Mohammad Jubair, Eleonora Cella, Massimo Ciccozzi, David J Nolan, William Farmerie, Mohammad H Rashid, Shrestha Sinha-Ray, Meer T Alam, J Glenn Morris, Marco Salemi
Vibrio cholerae is ubiquitous in aquatic environments, with environmental toxigenic V. cholerae O1 strains serving as a source for recurrent cholera epidemics and pandemic disease. However, a number of questions remain about long-term survival and evolution of V. cholerae strains within these aquatic environmental reservoirs. Through monitoring of the Haitian aquatic environment following the 2010 cholera epidemic, we isolated two novel non-toxigenic (ctxA/B-negative) Vibrio cholerae O1. These two isolates underwent whole-genome sequencing and were investigated through comparative genomics and Bayesian coalescent analysis...
October 27, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27727413/improvement-of-quality-of-speech-in-patients-with-velo-pharyngeal-insufficiency-corrected-using-a-buccinator-myomucosal-flap
#4
D K Dias, P D Fernando, R D Dissanayake
INTRODUCTION: Oro-facial clefts involving the palate is the commonest structural defect causing velopharyngeal insufficiency (VPI) and poor intelli gibility of speech. Proper repair of the soft palateis a surgical challenge. Posterior-based buccinator myomucosal flap (BMF) is used to lengthen the soft palate of patients who undergo primary palatoplasty at Teaching Hospital, Karapitiya (THK). BMF is a good choice for the repair of medium sized mucosal defects in the oral cavity since it has appropriate thickness, contains mucous membrane with mucous glands and has a rich blood supply...
2016: Ceylon Medical Journal
https://www.readbyqxmd.com/read/27639596/counterselection-employing-mutated-phes-for-markerless-genetic-deletion-in-bacteroides-species
#5
Yasuhiro Kino, Haruyuki Nakayama-Imaohji, Masashi Fujita, Ayano Tada, Saori Yoneda, Kazuya Murakami, Masahito Hashimoto, Tetsuya Hayashi, Katsuichiro Okazaki, Tomomi Kuwahara
Markerless gene deletion is necessary for multiple gene disruptions due to the limited number of antibiotic resistant markers for some bacteria. However, even in transformable strains, obtaining the expected mutation without a marker requires laborious screening of a large number of colonies. Previous studies had success in various bacteria with a counter-selection system where a conditional lethal gene was incorporated into the vector. We examined the efficacy of the mutated pheS gene (pheS*) as a counter-selective marker for gene deletion in Bacteroides...
September 14, 2016: Anaerobe
https://www.readbyqxmd.com/read/27619042/velopharyngeal-videofluoroscopy-providing-useful-clinical-information-in-the-era-of-reduced-dose-radiation-and-safety
#6
Pablo Antonio Ysunza, David Bloom, Kongkrit Chaiyasate, Matthew Rontal, Rachel VanHulle, Kenneth Shaheen, Donald Gibson
BACKGROUND: The state of the art for correcting velopharyngeal insufficiency (VPI) is a surgical procedure which is customized according to findings on imaging procedures: multiplanar videofluoroscopy (MPVF) and flexible videonasopharyngoscopy (FVNP). Recently, the use of MPVF has been challenged because of the potential risk of using ionizing radiation, especially in children. OBJECTIVE: To study whether using a protocol for performing MPVF can effectively decrease radiation dose in patients with VPI while providing useful information for planning surgical correction of VPI in combination with FVNP...
October 2016: International Journal of Pediatric Otorhinolaryngology
https://www.readbyqxmd.com/read/27585294/augmentation-pharyngoplasty-for-treatment-of-velopharyngeal-insufficiency-in-children-results-with-injectable-dextranomer-and-hyaluronic-acid-copolymer
#7
Shelagh A Cofer, Becky Baas, Edythe Strand, Cara C Cockerill
OBJECTIVES/HYPOTHESIS: To evaluate the effectiveness, durability, and safety of a tissue filler (dextranomer and hyaluronic acid copolymer) when injected submucosally in the nasopharynx to treat velopharyngeal insufficiency (VPI) in pediatric patients. STUDY DESIGN: Retrospective case series. METHODS: Charts were reviewed for all patients treated with injectable filler at a tertiary children's center for VPI from April 2010 through September 2013...
September 1, 2016: Laryngoscope
https://www.readbyqxmd.com/read/27563012/factors-influencing-parent-reports-of-facilitators-and-barriers-to-human-milk-supply-in-neonatal-intensive-care-units
#8
Elisabete Alves, Raquel Magano, Mariana Amorim, Conceição Nogueira, Susana Silva
BACKGROUND: Successful human milk supply in neonatal intensive care units (NICUs) requires the development of family-centered services. OBJECTIVE: This study aimed to assess parent perceptions of factors that help or hinder providing human milk to very preterm infants (VPI) in the NICU according to sociodemographic, reproductive, and obstetric characteristics. METHODS: This cross-sectional quantitative study included 120 mothers and 91 fathers of VPI hospitalized in a level 3 NICU located in the Northern Health Region of Portugal (July 2013-June 2014)...
August 25, 2016: Journal of Human Lactation: Official Journal of International Lactation Consultant Association
https://www.readbyqxmd.com/read/27517168/visceral-pleural-invasion-is-a-significant-prognostic-factor-in-patients-with-partly-solid-lung-adenocarcinoma-sized-30%C3%A2-mm-or-smaller
#9
Yangki Seok, Eungbae Lee
Background This study analyzed the impact of visceral pleural invasion (VPI) on the disease-free survival (DFS) of patients with partly solid pulmonary adenocarcinoma sized 30 mm or smaller. Method This is a retrospective study of 147 patients with surgically resected pathologic N0 pulmonary adenocarcinoma that had a partly solid appearance on preoperative computed tomography. All patients presented with tumors of size 30 mm or smaller. The DFS rate was estimated using Kaplan-Meier method. A multivariate analysis for prognostic factors was performed using the Cox proportional hazards regression model...
August 12, 2016: Thoracic and Cardiovascular Surgeon
https://www.readbyqxmd.com/read/27499970/visceral-pleural-invasion-in-lung-adenocarcinoma-%C3%A2-3-cm-with-ground-glass-opacity-a-clinical-pathological-and-radiological-study
#10
Li-Lan Zhao, Hui-Kang Xie, Li-Ping Zhang, Jun-Yan Zha, Fang-Yu Zhou, Ge-Ning Jiang, Chang Chen
BACKGROUND: Visceral pleural invasion (VPI) had been demonstrated as an aggressive sign in non-small cell lung cancers (NSCLC). However, its incidence and clinical relevance in early lung cancer showing ground glass nodules (GGNs) has not been clarified. METHODS: All consecutive surgically treated patients with solitary GGNs between 2009 and 2013 were reviewed retrospectively. Inclusion criteria were defined as lesions ≤3 cm with pleura abutting on computed tomography (CT) scan and pathologically confirmed NSCLC...
July 2016: Journal of Thoracic Disease
https://www.readbyqxmd.com/read/27497383/speech-outcomes-in-children-with-22q11-2-deletion-syndrome-following-surgery-for-velopharyngeal-insufficiency
#11
Pawina Jiramongkolchai, Manvinder S Kumar, Derrick Sowder, Sivakumar Chinnadurai, Christopher T Wootten, Steven L Goudy
OBJECTIVE: The purpose of this study was to identify prognostic factors associated with improved speech outcomes following surgical correction for velopharyngeal insufficiency (VPI) in pediatric patients with 22q11.2 deletion syndrome (22q11DS). METHODS: Eighteen patients were identified via retrospective chart review of patients with 22q11DS between 2005 and 2014. Patient characteristics, medical histories, associated comorbidities, surgical procedures, and pre- and postoperative perceptual hypernasality (subjectively rated 1-5 with 5 being the most severe) were gathered for each patient...
September 2016: International Journal of Pediatric Otorhinolaryngology
https://www.readbyqxmd.com/read/27473868/guidewire-exchange-vs-new-site-placement-for-temporary-dialysis-catheter-insertion-in-icu-patients-is-there-a-greater-risk-of-colonization-or-dysfunction
#12
Elisabeth Coupez, Jean-François Timsit, Stéphane Ruckly, Carole Schwebel, Didier Gruson, Emmanuel Canet, Kada Klouche, Laurent Argaud, Julien Bohe, Maïté Garrouste-Orgeas, Christophe Mariat, François Vincent, Sophie Cayot, Olivier Cointault, Alain Lepape, Michael Darmon, Alexandre Boyer, Elie Azoulay, Lila Bouadma, Alexandre Lautrette, Bertrand Souweine
BACKGROUND: Intensive care unit (ICU) patients require dialysis catheters (DCs) for renal replacement therapy (RRT). They carry a high risk of developing end-stage renal disease, and therefore their vascular access must be preserved. Guidewire exchange (GWE) is often used to avoid venipuncture insertion (VPI) at a new site. However, the impact of GWE on infection and dysfunction of DCs in the ICU is unknown. Our aim was to compare the effect of GWE and VPI on DC colonization and dysfunction in ICU patients...
July 30, 2016: Critical Care: the Official Journal of the Critical Care Forum
https://www.readbyqxmd.com/read/27442490/international-adoptees-with-cleft-lip-and-or-palate-lessons-learned-from-a-growing-population
#13
Paul Luther Shay, Jesse A Goldstein, Jason D Wink, J Thomas Paliga, Cynthia B Solot, Marilyn A Cohen, Susan A Friedman, David W Low, Jesse A Taylor, Oksana A Jackson
BACKGROUND: International adoptees with cleft lip and palate (CLP) are a growing population in the United States. They represent a clinical challenge, presenting at various ages and stages of cleft repair. METHODS: A retrospective review of patients seen at the CLP Program at the Children's Hospital of Philadelphia (CHOP) between 1998 and 2012 with a history of international adoption was performed. Demographics, surgical histories, and long-term speech outcomes were reviewed...
October 2016: Annals of Plastic Surgery
https://www.readbyqxmd.com/read/27389964/association-among-periodontitis-and-the-use-of-crack-cocaine-and-other-illicit-drugs
#14
Raquel P Antoniazzi, Fabricio B Zanatta, Cassiano K Rösing, Carlos Alberto Feldens
BACKGROUND: Crack cocaine can alter functions related to the immune system and exert a negative influence on progression and severity of periodontitis. The aim of this study is to compare periodontal status between crack cocaine users and crack cocaine non-users and investigate the association between crack cocaine and periodontitis after adjustments for confounding variables. METHODS: This cross-sectional study evaluated 106 individuals exposed to crack cocaine and 106 never exposed, matched for age, sex, and tobacco use...
December 2016: Journal of Periodontology
https://www.readbyqxmd.com/read/27260592/speech-characteristics-after-articulation-therapy-in-children-with-cleft-palate-and-velopharyngeal-dysfunction-a-single-case-experimental-design
#15
Fatemeh Derakhshandeh, Mohammadreza Nikmaram, Hedieh Hashemi Hosseinabad, Mehrdad Memarzadeh, Masoud Taheri, Mohammadreza Omrani, Shohreh Jalaie, Mahmood Bijankhan, Debbie Sell
OBJECTIVE: The aim of this study was to investigate the impact of an intensive 10-week course of articulation therapy on articulation errors in cleft lip and palate patients who have Velopharyngeal Insufficiency (VPI), non-oral and passive cleft speech characteristics. METHODS: Five children with cleft palate (+/-cleft lip) with VPI and non-oral and passive cleft speech characteristics underwent 40 intensive articulation therapies over 10 weeks in a single case experimental design...
July 2016: International Journal of Pediatric Otorhinolaryngology
https://www.readbyqxmd.com/read/27224571/oral-health-in-elders-with-parkinson-s-disease
#16
Giselle Rodrigues Ribeiro, Camila Heitor Campos, Renata Cunha Matheus Rodrigues Garcia
This study aimed to evaluate objectively and subjectively the oral health of elders with Parkinson's disease (PD), using clinical oral assessments and the General Oral Health Assessment Index (GOHAI). Subjects included 37 removable prosthesis wearers, 17 with PD (mean age 69.59±5.09 years) and 20 without PD (mean age 72.00±5.69 years). The objective assessment included an evaluation of oral characteristics, including the number of remaining teeth, decayed, missing and filled teeth (DMFT), visible plaque index (VPI), salivary flow rate and removable prosthesis conditions...
May 2016: Brazilian Dental Journal
https://www.readbyqxmd.com/read/27200238/orthognathic-consequences-of-sphincter-pharyngoplasty-in-cleft-patients-a-2-institutional-study
#17
Frances Yoshikane, Li Han Lai, Brian K Hui, Deborah B Martins, Gina Farias-Eisner, Rachel S Mandelbaum, Han Hoang, James P Bradley, Libby Wilson, Justine C Lee
BACKGROUND: Understanding long-term sequelae of cleft treatment is paramount in the refinement of treatment algorithms to accomplish optimized immediate and long-term outcomes. In this study, we reviewed sphincter pharyngoplasties as a method of velopharyngeal insufficiency (VPI) treatment in relationship to orthognathic surgery. METHODS: Cleft lip/palate and cleft palate patients, 15 years of age and older, were reviewed for demographics, VPI surgery, revisions, and subsequent orthognathic surgery at 2 institutions...
April 2016: Plastic and Reconstructive Surgery. Global Open
https://www.readbyqxmd.com/read/27178776/visceral-pleural-invasion-impacts-the-prognosis-of-non-small-cell-lung-cancer-a-meta-analysis
#18
Q-X Liu, X-F Deng, D Zhou, J-M Li, J-X Min, J-G Dai
BACKGROUND: In the 7th tumor, node, metastasis (TNM) classification, T1 tumors with visceral pleural invasion (VPI) are upgraded to T2a. The objective of this study was to evaluate the prognostic impact of VPI among patients with NSCLC and to propose a method of incorporating VPI into T-status classification in future staging systems. METHODS: A systematic electronic search was conducted from each database's date of inception to October 2015. The included studies were selected according to predefined inclusion criteria...
November 2016: European Journal of Surgical Oncology
https://www.readbyqxmd.com/read/27145594/-vaccine-associated-paralytic-poliomyelitis-in-russian-federation-during-the-period-of-changes-in-vaccination-schedule-2006-2013-yy
#19
O E Ivanova, T P Eremeeva, N S Morozova, A K Shakaryan, A P Gmyl, M L Yakovenko, E A Korotkova, O P Chernjavskaja, O Yu Baykova, O V Silenova, A Yu Krasota, L I Krasnoproshina, A N Mustafina, L I Kozlovskaja
The results of virologic testing of clinical materials and epidemiological analysis of vaccine-associated paralytic poliomyelitis (VAPP) cases obtained in 2006-2013 during AFP surveillance are presented. Among the 2976 cases of AFP 30 cases were VAPP. 15 cases were observed in OPV recipients, whereas 15 cases were observed in non-vaccinated contacts. The age of the patients varied from 4 months to 5.5 years (13.6 ± 12.4 months old). Children younger than 1 year constituted 63.3% of the group; boys were dominant (73...
2016: Voprosy Virusologii
https://www.readbyqxmd.com/read/27143929/the-predictive-and-prognostic-values-of-factors-associated-with-visceral-pleural-involvement-in-resected-lung-adenocarcinomas
#20
Huibiao Zhang, Chen Lu, Yingjie Lu, Bo Yu, Fanzhen Lv, Zhenghong Zhu
BACKGROUND: The predictive and prognostic impact of factors associated with visceral pleural invasion (VPI) on survival and recurrence in patients with resected lung adenocarcinomas is not clearly defined. PATIENTS AND METHODS: A total of 505 consecutive patients with stage Ia-IIIa lung adenocarcinomas treated with radical resection were included. The predominant growth pattern was classified according to the new classification system for lung adenocarcinoma proposed by the International Association for the Study of Lung Cancer, the American Thoracic Society, and the European Respiratory Society...
2016: OncoTargets and Therapy
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