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Bassem Khemakhem, Slim Smaoui, Hanen El Abed, Imen Fendri, Hajer Hammami, Mohamed Ali Ayadi
Three different concentrations of a purified maltogenic amylase (FSA) from fenugreek ( Trigonella foenum graecum ) seeds were incorporated into the cake formulation. The addition of FSA at 0.003, 0.005 and 0.01 U/g of cake increased the loaf volume, the number of holes (gas cells), and water absorption. Textural study revealed an improvement of the cake quality, resulting in the decrease of hardness and the increase of cohesion. Environmental scanning electron microscopy was performed on different cakes to evaluate the influence of amylase activity on microstructure...
March 2018: 3 Biotech
Wafa Hassen, Mohamed Neifar, Hanene Cherif, Afef Najjari, Habib Chouchane, Rim C Driouich, Asma Salah, Fatma Naili, Amor Mosbah, Yasmine Souissi, Noura Raddadi, Hadda I Ouzari, Fabio Fava, Ameur Cherif
A number of Pseudomonas strains function as inoculants for biocontrol, biofertilization, and phytostimulation, avoiding the use of pesticides and chemical fertilizers. Here, we present a new metabolically versatile plant growth-promoting rhizobacterium, Pseudomonas rhizophila S211, isolated from a pesticide contaminated artichoke field that shows biofertilization, biocontrol and bioremediation potentialities. The S211 genome was sequenced, annotated and key genomic elements related to plant growth promotion and biosurfactant (BS) synthesis were elucidated...
2018: Frontiers in Microbiology
Najla El-Hachem, Nadia Habel, Tanesha Naiken, Hanene Bzioueche, Yann Cheli, Guillaume E Beranger, Emilie Jaune, Florian Rouaud, Nicolas Nottet, Frédéric Reinier, Céline Gaudel, Pascale Colosetti, Corine Bertolotto, Robert Ballotti
HACE1 is an E3 ubiquitin ligase described as a tumour suppressor because HACE1-knockout mice develop multi-organ, late-onset cancers and because HACE1 expression is lost in several neoplasms, such as Wilms' tumours and colorectal cancer. However, a search of public databases indicated that HACE1 expression is maintained in melanomas. We demonstrated that HACE1 promoted melanoma cell migration and adhesion in vitro and was required for mouse lung colonisation by melanoma cells in vivo. Transcriptomic analysis of HACE1-depleted melanoma cells revealed an inhibition of ITGAV and ITGB1 as well changes in other genes involved in cell migration...
March 7, 2018: Cell Death and Differentiation
Najla Mechergui, Nesrine Chaouech, Hanéne Ben Said, Mejda Bani, Radhia Boujday, Nizar Ladhari
INTRODUCTION: The evaluation of fitness for work can be conducted upon the employer's request even during the employee's sickness absence. AIM: Determine the contribution of conducting medical examinations to assess the working ability of Charles Nicolle hospital's workers who are on sick leave. METHODS: A descriptive and retrospective study included all the medical records, completed over a two-year period (2013-2014), of Charles Nicolle hospital's personnel who were on sick leave and who consulted the service of occupational medicine and professional pathology to undergo a fitness for work evaluation requested by the employer...
May 2017: La Tunisie Médicale
Hanen Ghazali, Rania Jebri, Anware Yahmadi, Najla El Heni, Ines Chermiti, Sami Souissi
BACKGROUND: The objective of our study was to evaluate the predictors of repeat visits at Emergency Department (ED) in patients aged ≥ 65 years. METHODS: A prospective observational study was conducted over a month. Inclusion:  patients (age> = 65 year) seen in the ED and leaving the same day. The epidemiological and clinical characteristics at the first visit (index visit (IV)) were collected. The Identification of Senior At Risk (ISAR) score was used to assess our patients...
May 2017: La Tunisie Médicale
Habib Mosbah, Hassiba Chahdoura, Jannet Kammoun, Malek Besbes Hlila, Hanen Louati, Saoussen Hammami, Guido Flamini, Lotfi Achour, Boulbaba Selmi
BACKGROUND: α-glucosidase is a therapeutic target for diabetes mellitus (DM) and α-glucosidase inhibitors play a vital role in the treatments for the disease. Furthermore, xanthine oxidase (XO) is a key enzyme that catalyzes hypoxanthine and xanthine to uric acid which at high levels can lead to hyperuricemia which is an important cause of gout. Pancreatic lipase (PL) secreted into the duodenum plays a key role in the digestion and absorption of fats. For its importance in lipid digestion, PL represents an attractive target for obesity prevention...
March 5, 2018: BMC Complementary and Alternative Medicine
Najeh Krayem, Goetz Parsiegla, Hélène Gaussier, Hanen Louati, Raida Jallouli, Pascal Mansuelle, Frédéric Carrière, Youssef Gargouri
BACKGROUND: Heterodimeric phospholipase A2 from venom glands of Tunisian scorpion Scorpio maurus (Sm-PLGV) had been purified. It contains long and short chains linked by a disulfide bridge. Sm-PLGV exhibits hemolytic activity towards human erythrocytes and interacts with phospholipid monolayers at high surface pressure. The investigation of structure-function relationships should provide new clues to understand its activity. METHODS: Molecular cloning of Sm-PLGV and heterologous expression in Escherichia coli of three recombinant forms was used to determine the role of the short chain on enzymatic activity...
February 22, 2018: Biochimica et Biophysica Acta
Naima Boutaghane, Abdulmagid Alabdul Magid, Amin Abedini, Anaïs Cafolla, Hanène Djeghim, Sophie C Gangloff, Laurence Voutquenne-Nazabadioko, Zahia Kabouche
A previously undescribed triterpenoid saponin, 3-O-[α-l-rhamnopyranosyl-(1→2)-{β-d-glucopyranosyl-(1→6)-}β-d-galactopyranosyl-(1→2)-β-d-glucuronopyranosyl]-sophoradiol (1), in addition to twenty-nine known constituents (2-30) were isolated from the aerial parts of Genista numidica Spach. Structures elucidation was performed by comprehensive 1D- and 2D-NMR analyses and HRESIMS. The extracts, fractions and isolated compounds were evaluated for their antibacterial, antioxidant and tyrosinase inhibitory activities...
February 15, 2018: Natural Product Research
Hanen Boukedi, Slim Tounsi, Lobna Abdelkefi-Mesrati
The carob moth, Ectomyelois ceratoniae, is an important agricultural pest that is susceptible to the Vip3(459) protein. The insecticidal activity, evaluated against this lepidopteran pest, displayed an LC 50 value of about 28 ng/cm 2 .The investigation of the mode of action of this B. thuringiensis protein demonstrated that the active form of this toxin bound to putative receptors in the BBMV of E. ceratoniae. Ligand blotting experiment proved that Vip3(459) specifically bound to two proteins of about 53 and 57 kDa, located on the midgut...
February 12, 2018: Acta Tropica
Hanène Bouzidi, Aurélie Emirian, Antoine Marty, Elisabeth Chachaty, Agnès Laplanche, Bertrand Gachot, François Blot
OBJECTIVES: Differential time to positivity of cultures of blood drawn simultaneously from central venous catheter and peripheral sites is widely used to diagnose catheter-related bloodstream infections without removing the catheter. However, the accuracy of this technique for some pathogens such as Staphylococcus aureus is debated in routine practice. METHODS: In a 320-bed reference cancer centre, we retrospectively studied the charts of patients with at least one blood culture positive for S...
February 9, 2018: Journal of Hospital Infection
Rabeb Ghali, Maryam A Al-Mutawa, Abrar K Al-Ansari, Sonia Zaied, Hanen Bhiri, Touhami Mahjoub, Wassim Y Almawi
BACKGROUND: Estrogen is key to breast cancer pathogenesis, and acts by binding its receptor (ER), which exists as ERα and ERβ, encoded by ESR1 and ESR2 genes, respectively. Studies that investigated the association of ESR1 and ESR2 variants with breast cancer yielded mixed outcome, and ethnic contribution was proposed. We evaluated the association between ESR1 and ESR2 variants and breast cancer and associated features in Tunisian women. METHODS: Retrospective case-control study involving 207 female breast cancer patients, and 284 control women...
February 4, 2018: Gene
Mariam Siala, Markus Rihl, Hanen Sellami, Abir Znazen, Nadia Sassi, Lilia Laadhar, Radhouane Gdoura, Imen Belghuith, Dalila Mrabet, Sofien Baklouti, Slaheddine Sellami, Jean Sibilia, Hela Fourati, Adnene Hammami, Ilhem Cheour
Enterobacterial components in the joints of patients are believed to contribute to a perpetuating inflammation leading to a reactive arthritis (ReA), a condition in which microbial agents cannot be recovered from the joint. At present, it is unclear whether nucleic acids from Shigella spp. are playing a pathogenic role in causing not only ReA but also other forms of arthritis. Quantitative real-time polymerase chain reaction assay (qPCR) is the method of choice for the identification of bacteria within the synovium...
February 5, 2018: Rheumatology International
Mourad Ben Said, Hanène Belkahia, Lilia Messadi
The genus Anaplasma belonging to the Anaplasmataceae family (order Rickettsiales) comprises obligate intracellular Gram-negative bacteria of veterinary and public health importance. Six species and five types of strains genetically related are currently assigned to the genus Anaplasma including Anaplasma marginale, A. centrale, A. bovis, A. phagocytophilum, A. ovis and A. platys as classified species, and "A. capra", A. odocolei sp. nov., A. phagocytophilum-like 1 (Anaplasma sp.-Japan), A. phagocytophilum-like 2 (Anaplasma sp...
February 1, 2018: Ticks and Tick-borne Diseases
Hanen Boukedi, Saoussen Ben Khedher, Lobna Abdelkefi-Mesrat, Jeroen Van Rie, Slim Tounsi
The cotton leaf worm Spodoptera littoralis is known for causing serious damages to various crops. In this study, the susceptibility/tolerance of this larvae to four Vip3A (Vip3Aa, Vip3Ae, Vip3Ad and Vip3Af) toxins was investigated. UnlikeVip3Ad which showed no activity to S. littoralis, Vip3Aa, Vip3Ae and Vip3Af exhibited high toxicity to this larva with LC50 of 228.42 ng/cm2, 65.71 ng/cm2, and 388.90 ng/cm2, respectively. Activation of the 90 kDa Vip3A proteins by S. littoralis larvae juice generated four major bands of sizes 62, 45, 33 and 22 kDa...
January 26, 2018: Journal of Invertebrate Pathology
Zaied Sonia, Daldoul Amira, Bhiri Hanene, Ammar Nouha, Khechine Wiem, Toumi Omar, Abbes Ibtissem, Ben Salem Amina, Njima Manel, Mhabrech Houda
Squamous cell carcinoma (SCC) of the rectum is a rare disease. A 59-year-old man presented with SCC of the middle rectum located 10 cm from the anus. The stage of the tumor was revealed to be T3N+M0. The patient received a combined treatment with cisplatin and fluorouracil in concomitance with external radiation therapy. He then underwent an anterior resection of the rectum. The postoperative histopathological findings classified the tumor as ypT0N0 with cancer-free margins and lymph nodes. Treatment of SCC remains very challenging, and the acquisition of more consistent data is needed...
November 2017: Cancer Biology & Medicine
Hanen Khabou, Marcela Garita-Hernandez, Antoine Chaffiol, Sacha Reichman, Céline Jaillard, Elena Brazhnikova, Stéphane Bertin, Valérie Forster, Mélissa Desrosiers, Céline Winckler, Olivier Goureau, Serge Picaud, Jens Duebel, José-Alain Sahel, Deniz Dalkara
Intraocular injection of adeno-associated viral (AAV) vectors has been an evident route for delivering gene drugs into the retina. However, gaps in our understanding of AAV transduction patterns within the anatomically unique environments of the subretinal and intravitreal space of the primate eye impeded the establishment of noninvasive and efficient gene delivery to foveal cones in the clinic. Here, we establish new vector-promoter combinations to overcome the limitations associated with AAV-mediated cone transduction in the fovea with supporting studies in mouse models, human induced pluripotent stem cell-derived organoids, postmortem human retinal explants, and living macaques...
January 25, 2018: JCI Insight
Rym Essid, Jean Menotti, Chelbi Hanen, Karim Aoun, Aïda Bouratbine
Cryptosporidium is an enteric parasite infecting a wide range of hosts. It has emerged as an important cause of chronic life-threatening diarrhea in humans worldwide. Several subtypes of Cryptosporidium sp. have been described to be responsible for several large outbreaks related to water contamination in developed countries. However, there is a lack of information in the genetic diversity of Cryptosporidium among human population especially in developing countries. The present study aimed to update and report the genetic diversity of human Cryptosporidium spp...
January 7, 2018: Infection, Genetics and Evolution
Tahani Louhichi, Hanene Saad, Myriam Ben Dhiab, Sonia Ziadi, Mounir Trimeche
BACKGROUND: Previous investigations have indicated that CD10 is associated with biological aggressivity in human cancers, but the use of this marker for diagnosis and prognosis is more complex. The aim of this study was to evaluate the expression of CD10 in breast cancer and its association with the clinicopathological features. In addition, we investigated whether a relationship exists between CD10 expression and cancer stem cells. METHODS: CD10 expression was examined by the immunohistochemistry in a series of 133 invasive breast carcinoma cases...
January 6, 2018: BMC Cancer
Hanen Chelbi, Rym Essid, Refka Jelassi, Nesrine Bouzekri, I Zidi, Hamza Ben Salah, Ilhem Mrad, Iness Ben Sgaier, Rym Abdelmalek, Sameh Aissa, Aida Bouratbine, Karim Aoun
BACKGROUND: Cryptosporidiosis represents a major public health problem. This infection, caused by a protozoan parasite of the genus Cryptosporidium, has been reported worldwide as a frequent cause of diarrhoea. In the immunocompetent host, the typical watery diarrhea can be self-limiting. However, it is severe and chronic, in the immunocompromised host and may cause death. Cryptosporidium spp. are coccidians, which complete their life cycle in both humans and animals. The two species C...
January 3, 2018: Microbial Pathogenesis
Wafa Jallouli, Hanen Boukadi, Sameh Sellami, Fakher Frikha, Lobna Abdelkefi-Mesrati, Slim Tounsi
The entomopathogenic Photorhabdus luminescens TT01 is a promoting bacterium that controls effectively many insect pests. Indeed, it exhibited a mortality rate of 32.36% against the first instar larvae of the turnip moth Agrotis segetum, when it was used at a concentration of 5 × 107 cells/ml but no toxicity against the second instar larvae in the same condition. P. luminescens TT01 oral toxicity is associated to septicaemia since cells fraction exhibited the highest mortality rate of 34%. In order to enhance P...
January 2, 2018: Toxicon: Official Journal of the International Society on Toxinology
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