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https://www.readbyqxmd.com/read/28922729/lithium-induced-oxidative-damage-and-inflammation-in-the-rat-s-heart-protective-effect-of-grape-seed-and-skin-extract
#1
Ali Mezni, Hanène Aoua, Olfa Khazri, Ferid Limam, Ezzeddine Aouani
Lithium (Li) is a relevant mood stabilizer metal for the treatment of bipolar disorder (BD), as it protects from both depression and mania and reduces the risk of suicide. However, Lihas some clinical concerns as a narrow therapeutic index requiring routine monitoring of the serum level. The present study was designed to analyze the cardio-toxic side effect of Li and the ability of grape seed and skin extract (GSSE) to protect the heart against such toxicity. After 30days of exposure to Li (0, 2, 5 and 100mg/kg bw) and prevention with GSSE (4000mg/kg bw), rats were killed by decapitation and their heart processed for Li-induced oxidative stress...
September 14, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28920905/electrochemical-impedance-spectroscopy-on-interdigitated-gold-microelectrodes-for-glycosylated-human-serum-albumin-characterization
#2
Nadra Bohli, Hanen Chammem, Olivier Meilhac, Laurence Mora, Adnane Abdelghani
Glycosylated albumin is considered as a potentially accurate indicator of shorter-term average glucose concentration compared to the current standard HbA1c and as such, it's attracting the interest of the scientific community as a possible diagnosis marker for diabetic patients. The purpose of this paper is to achieve a better understanding of the glycation effect of albumin on its electrochemical properties. That is done through the use of Interdigitated gold microelectrodes (IDGE) as support in a label free impedimetric immunosensor for detection of human serum albumin detection in glycated (GA) and non-glycated (HSA) form...
September 14, 2017: IEEE Transactions on Nanobioscience
https://www.readbyqxmd.com/read/28920707/in-vitro-and-in-vivo-studies-of-allium-sativum-extract-against-deltamethrin-induced-oxidative-stress-in-rats-brain-and-kidney
#3
Marwa Ncir, Mongi Saoudi, Hanen Sellami, Fatma Rahmouni, Amina Lahyani, Fatma Makni Ayadi, Abdelfattah El Feki, Mohamed Salah Allagui
The present study investigated the in vitro and the in vivo antioxidant capacities of Allium sativum (garlic) extract against deltamethrin-induced oxidative damage in rat's brain and kidney. The in vitro result showed that highest extraction yield was achieved with methanol (20.08%). Among the tested extracts, the methanol extract exhibited the highest total phenolic, flavonoids contents and antioxidant activity. The in vivo results showed that deltamethrin treatment caused an increase of the acetylcholinesterase level (AChE) in brain and plasma, the brain and kidney conjugated dienes and lipid peroxidation (LPO) levels as compared to control group...
September 18, 2017: Archives of Physiology and Biochemistry
https://www.readbyqxmd.com/read/28906158/prevalence-of-putative-virulence-genes-in-campylobacter-and-arcobacter-species-isolated-from-poultry-and-poultry-by-products-in-tunisia
#4
Hela Jribi, Hanen Sellami, Amal Ben Hassena, Radhouane Gdoura
Campylobacter and Arcobacter spp. are common causes of gastroenteritis in humans; these infections are commonly due to undercooked poultry. However, their virulence mechanism is still poorly understood. The aim of this study was to evaluate the presence of genotypic virulence markers in Campylobacter and Arcobacter species using PCR. The prevalence of virulence and cytolethal distending toxin (CDT) genes was estimated in 71 Campylobacteraceae isolates. PCR was used to detect the presence of virulence genes (iam, cadF, virB1, flaA, cdtA, cdtB, and cdtC) using specific primers for a total of 45 Campylobacter isolates, including 37 C...
September 14, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28888642/spatio-temporal-variations-and-genetic-diversity-of-anaplasma-spp-in-cattle-from-the-north-of-tunisia
#5
Hanène Belkahia, Mourad Ben Said, Narjesse El Mabrouk, Mariem Saidani, Chayma Cherni, Mariem Ben Hassen, Ali Bouattour, Lilia Messadi
In cattle, anaplasmosis is a tick-borne rickettsial disease caused by Anaplasma marginale, A. centrale, A. phagocytophilum, and A. bovis. To date, no information concerning the seasonal dynamics of single and/or mixed infections by different Anaplasma species in bovines are available in Tunisia. In this work, a total of 1035 blood bovine samples were collected in spring (n=367), summer (n=248), autumn (n=244) and winter (n=176) from five different governorates belonging to three bioclimatic zones from the North of Tunisia...
September 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28875714/effects-of-co-exposure-to-imidacloprid-and-gibberellic-acid-on-redox-status-kidney-variables-and-histopathology-in-adult-rats
#6
Bornia Lafi, Mariem Chaâbane, Awatef Elwej, Malek Grati, Kamel Jamoussi, Hela Mnif, Tahia Boudawara, Hanen Ketata Bouaziz, Najiba Zeghal
Data on the individual nephrotoxic effects of imidacloprid (IMI) and gibberellic acid (GA3) are scarce. Moreover, there is a lack of information about their combined effects on the renal tissue. Our study investigated the effects of IMI and GA3 separately or together on rats kidney. IMI (64 mg/kg bw) was given for 3 weeks by gavage either individually or in combination with GA3 (200 mg/L) via drinking water. IMI associated or no with GA3 increased the levels of kidney malondialdehyde, advanced oxidation protein products, protein carbonyls and metallothionein, plasma creatinine, urea, blood urea nitrogen and lactate dehydrogenase activity...
September 6, 2017: Archives of Physiology and Biochemistry
https://www.readbyqxmd.com/read/28873321/separate-and-combined-effects-of-time-of-day-and-verbal-instruction-on-knee-extensor-neuromuscular-adjustments
#7
Hamdi Jaafar, Olivier Girard, Hanene Lajili
We examined time of day and verbal instruction separate and combined effects on knee extensor neuromuscular adjustments, with special reference to rapid muscle force production capacity. Ten healthy male participants performed four experimental trials in counterbalanced order: morning-"hard-and-fast" instruction, evening-"hard-and-fast" instruction, morning-"fast" instruction and evening-"fast" instruction. During each experimental trial, neuromuscular performance was assessed from the completion of six maximal isometric voluntary contractions (rest = 2 minutes) of the knee extensors with concomitant quadriceps surface EMG recordings...
September 5, 2017: Applied Physiology, Nutrition, and Metabolism, Physiologie Appliquée, Nutrition et Métabolisme
https://www.readbyqxmd.com/read/28871244/microbial-diversity-in-sulfate-reducing-marine-sediment-enrichment-cultures-associated-with-anaerobic-biotransformation-of-coastal-stockpiled-phosphogypsum-sfax-tunisia
#8
Hana Zouch, Fatma Karray, Fabrice Armougom, Sandrine Chifflet, Agnès Hirschler-Réa, Hanen Kharrat, Lotfi Kamoun, Wajdi Ben Hania, Bernard Ollivier, Sami Sayadi, Marianne Quéméneur
Anaerobic biotechnology using sulfate-reducing bacteria (SRB) is a promising alternative for reducing long-term stockpiling of phosphogypsum (PG), an acidic (pH ~3) by-product of the phosphate fertilizer industries containing high amounts of sulfate. The main objective of this study was to evaluate, for the first time, the diversity and ability of anaerobic marine microorganisms to convert sulfate from PG into sulfide, in order to look for marine SRB of biotechnological interest. A series of sulfate-reducing enrichment cultures were performed using different electron donors (i...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28871008/can-health-indicators-and-psychosocial-characteristics-predict-attrition-in-youths-with-overweight-and-obesity-seeking-ambulatory-treatment-data-from-a-retrospective-longitudinal-study-in-a-paediatric-clinic-in-luxembourg
#9
Ineke M Pit-Ten Cate, Hanen Samouda, Ulrike Schierloh, Julien Jacobs, Jean Francois Vervier, Saverio Stranges, Marie Lise Lair, Carine de Beaufort
OBJECTIVES: The current study aimed to identify factors that could predict attrition in youths starting ambulatory treatment to control or lose weight. DESIGN: Retrospective longitudinal study. SETTING: Paediatric clinic: ambulatory treatment programme. PATIENTS AND MEASURES: A youth sample (n=191; 89 boys; aged 7-17 years) completed measures of demographic characteristics, and health and psychosocial traits before starting an ambulatory weight management programme...
September 3, 2017: BMJ Open
https://www.readbyqxmd.com/read/28855021/elimination-of-escherichia-coli-and-salmonella-in-clam-by-using-zeolite-in-a-station-of-depuration
#10
Morsi Gdoura, Hanen Sellami, Lamia Khannous, Najib Ketata, Idriss Ben Neila, Al Ibrahim Traore, Zouhair Chekir, Radhouane Gdoura
  The application of natural zeolite for water and wastewater treatment has been carried out and is still a promising technique in environmental cleaning processes. Natural zeolite can be used to improve the purification process of clams (Ruditapes decussatus). Thus, our study aimed at improving the clam purification system in order to reduce Escherichia coli and eliminate Salmonella in samples artificially contaminated with this bacterium using a natural zeolite to replace the biological filter. The results showed that zeolite used in a depuration system improved the clam purification process...
September 1, 2017: Water Environment Research: a Research Publication of the Water Environment Federation
https://www.readbyqxmd.com/read/28853632/isolation-and-identification-of-campylobacter-spp-from-poultry-and-poultry-by-products-in-tunisia-by-conventional-culture-method-and-multiplex-real-time-pcr
#11
Hela Jribi, Hanen Sellami, Siala Mariam, Salma Smaoui, Asma Ghorbel, Salma Hachicha, Lucie Benejat, Feriel Messadi-Akrout, Francis Mégraud, Radhouane Gdoura
Thermophilic Campylobacter spp. are one of the primary causes of bacterial human diarrhea. The consumption of poultry meats, by-products, or both is suspected to be a major cause of human campylobacteriosis. The aims of this study were to determine the prevalence of thermophilic Campylobacter spp. in fresh poultry meat and poultry by-products by conventional culture methods and to confirm Campylobacter jejuni and Campylobacter coli isolates by using the multiplex PCR assay. Two hundred fifty fresh poultry samples were collected from a variety of supermarkets and slaughterhouses located in Sfax, Tunisia, including chicken (n =149) and turkey (n =101)...
August 30, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28836054/molecular-study-of-abcb1-gene-and-its-correlation-with-imatinib-response-in-chronic-myeloid-leukemia
#12
Islem Ben Hassine, Hanene Gharbi, Ismail Soltani, Hind Ben Hadj Othman, Ahlem Farrah, Hassiba Amouri, Mouheb Teber, Hela Ghedira, Yosra Ben Youssef, Ines Safra, Salem Abbes, Samia Menif
PURPOSE: The introduction and success of imatinib mesylate have become a paradigm shift in chronic myeloid leukemia (CML) treatment. However, despite its high efficiency, resistance to imatinib has emerged as a significant problem, which may in part be caused by pharmacogenetic variability. Three single-nucleotide polymorphisms (C1236T, G2677T/A, C3435T) and/or mRNA expression changes of ABCB1 gene were demonstrated to be associated with inter-individual variability of imatinib response in CML patients...
August 23, 2017: Cancer Chemotherapy and Pharmacology
https://www.readbyqxmd.com/read/28800636/combined-sprint-and-resistance-training-abrogates-age-differences-in-somatotropic-hormones
#13
Maha Sellami, Wissem Dhahbi, Lawrence D Hayes, Johnny Padulo, Fatma Rhibi, Hanen Djemail, Anis Chaouachi
The aim of this investigation was to compare serum growth hormone (GH), insulin-like growth factor-1 (IGF-1) and insulin-like growth factor-binding protein-3 (IGFBP-3) in response to a combined sprint and resistance training (CSRT) program in young and middle-aged men.Thirty-eight healthy, moderately trained men participated in this study. Young and middle-aged men were randomly assigned to, a young training group (YT = 10, 21.4±1.2yrs) ora young control group (YC = 9, 21.6±1.8 yrs), a middle-aged training group (MAT = 10, 40...
2017: PloS One
https://www.readbyqxmd.com/read/28800521/potassium-deficiency-alters-growth-photosynthetic-performance-secondary-metabolites-content-and-related-antioxidant-capacity-in-sulla-carnosa-grown-under-moderate-salinity
#14
Chokri Hafsi, Hanen Falleh, Mariem Saada, Riadh Ksouri, Chedly Abdelly
Salinity and K(+) deficiency are two environmental constraints that generally occur simultaneously under field conditions, resulting in severe limitation of plant growth and productivity. The present study aimed at investigating the effects of salinity, either separately applied or in combination with K(+) deficiency, on growth, photosynthetic performance, secondary metabolites content, and related antioxidant capacity in Sulla carnosa. Seedlings were grown hydroponically under sufficient (6000 μM) or low (60 μM) K(+) supply with 100 mM NaCl (C + S and D + S treatments, respectively)...
August 4, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28793955/optimization-of-therapeutic-drug-monitoring-of-vancomycin-in-patients-with-chronic-hemodialysis%C3%A2
#15
Lobna Ben Mahmoud, Yosr Chaabouni, Hanen Ghozzi, Houda Feriani, Ahmed Hakim, Mahmoud Kharrat, Chakib Marrakchi, Zouheir Sahnoun, Mounir Ben Jmaa, Khaled Zeghal, Jamil Hachicha
PURPOSE: To validate a simplified vancomycin monitoring algorithm in patients on chronic hemodialysis who required intravenous vancomycin for at least 3 weeks. MATERIALS AND METHODS: In this prospective study, all hemodialysis patients who were admitted between April 1, 2013, and March 31, 2015, in our unit for suspected or confirmed methicillin-resistant Staphylococcus aureus infection that required vancomycin were enrolled. All patients were categorized into two groups...
August 10, 2017: Clinical Nephrology
https://www.readbyqxmd.com/read/28774465/a-new-preprocessing-parameter-estimation-based-on-geodesic-active-contour-model-for-automatic-vestibular-neuritis-diagnosis
#16
Amine Ben Slama, Aymen Mouelhi, Hanene Sahli, Sondes Manoubi, Chiraz Mbarek, Hedi Trabelsi, Farhat Fnaiech, Mounir Sayadi
The diagnostic of the vestibular neuritis (VN) presents many difficulties to traditional assessment methods This paper deals with a fully automatic VN diagnostic system based on nystagmus parameter estimation using a pupil detection algorithm. A geodesic active contour model is implemented to find an accurate segmentation region of the pupil. Hence, the novelty of the proposed algorithm is to speed up the standard segmentation by using a specific mask located on the region of interest. This allows a drastically computing time reduction and a great performance and accuracy of the obtained results...
July 2017: Artificial Intelligence in Medicine
https://www.readbyqxmd.com/read/28694749/variability-of-antioxidant-and-biological-activities-of-rhus-tripartitum-related-to-phenolic-compounds
#17
Hanène Ben Miled, Mariem Saada, Ines Jallali, Zaineb Ben Barka, Mounira Tlili, Hichem Alimi, Mohsen Sakly, Khémais Ben Rhouma, Manef Abderrabba, Hafedh Abdelmelek, Olfa Tebourbi, Riadh Ksouri
Rhus species are known in traditional medicine for their therapeutic virtue and their extracts showed numerous important properties including antimalarial, antimicrobial, antiviral, and hypoglycemic and anticonvulsant activities. Rhus tripartitum (Ucria) is a medicinal plant widely used in Tunisia folk medicine against chronic diarrhea and gastric ulcer. This study was designed to examine in vitro and ex vivo antioxidant, anti-inflammatory and anticancer activities of four extracts of Rhus tripartitum root cortex with increasing solvent polarity (hexane, dichloromethane, methanol and water)...
2017: EXCLI journal
https://www.readbyqxmd.com/read/28679351/modulatory-effects-of-quercetin-on-liver-histopathological-biochemical-hematological-oxidative-stress-and-dna-alterations-in-rats-exposed-to-graded-doses-of-score-250
#18
Sabrine Kasmi, Intithar Bkhairia, Bahira Harrabi, Hela Mnif, Rim Marrakchi, Hanen Ghozzi, Choumous Kallel, Moncef Nasri, Khaled Zeghal, Kamel Jamoussi, Ahmed Hakim
This study investigated the morphological, biochemical and molecular aspects of liver injury in rats after the exposure to difenoconazole and the protective effects of quercetin against hepatotoxicity and genotoxicity induced by this fungicide. Rats were given graded doses of difenoconazole associated or not to quercetin daily for 20 days. Our results showed a significant increase in PLT (Platelets) and WBC (white blood cells) in rats treated with higher doses of difenoconazole (1/38 and 1/9 of LD50). However, a significant decrease in Hb (Hemoglobin) rate and RBC (Red blood cells) number in rats treated with higher doses of difenoconazole (1/38 and 1/9 of LD50) was obtained...
July 5, 2017: Toxicology Mechanisms and Methods
https://www.readbyqxmd.com/read/28666229/structural-characterization-and-identification-of-cyclic-lipopeptides-produced-by-bacillus-methylotrophicus-dcs1-strain
#19
Nawel Jemil, Angeles Manresa, Francesc Rabanal, Hanen Ben Ayed, Noomen Hmidet, Moncef Nasri
Bacillus methylotrophicus DCS1 strain was isolated from diesel contaminated soil and screened for its ability to produce biosurfactants; it was found effective for the production of surface active molecules. The structural characterization of the isolated lipopeptides was studied by a variety of analytical techniques. The organic extract of DCS1 lipopeptides was fractionated by silica gel column chromatography (60Mesh). Fractions containing lipopeptides were collected and identified by tandem mass spectrometry MALDI-TOF-MS and MALDI-TOF MS(2)...
August 15, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28659164/antioxidant-properties-antimicrobial-and-anti-adhesive-activities-of-dcs1-lipopeptides-from-bacillus-methylotrophicus-dcs1
#20
Nawel Jemil, Hanen Ben Ayed, Angeles Manresa, Moncef Nasri, Noomen Hmidet
BACKGROUND: The present work aims to investigate the antioxidant and antimicrobial activities as well as the potential of DCS1 lipopeptides produced by Bacillus methylotrophicus DCS1 strain at inhibition and disruption of biofilm formation. RESULTS: The produced biosurfactants were characterized as lipopeptides molecules by using thin layer chromatography (TLC) and Fourier transform infrared spectroscopy (FT-IR). The DCS1 lipopeptides were assayed for their antioxidant activity through five different tests...
June 28, 2017: BMC Microbiology
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