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Mourad Ben Said, Alaa Ben Asker, Hanène Belkahia, Raoua Ghribi, Rachid Selmi, Lilia Messadi
Anaplasma marginale, which is responsible for bovine anaplasmosis in tropical and subtropical regions, is a tick-borne obligatory intraerythrocytic bacterium of cattle and wild ruminants. In Tunisia, information about the genetic diversity and the phylogeny of A. marginale strains are limited to the msp4 gene analysis. The purpose of this study is to investigate A. marginale isolates infecting 16 cattle located in different bioclimatic areas of northern Tunisia with single gene analysis and multilocus sequence typing methods on the basis of seven partial genes (dnaA, ftsZ, groEL, lipA, secY, recA and sucB)...
May 12, 2018: Ticks and Tick-borne Diseases
Hamza Ben Yahia, Wafa Babay, Daria Bortolotti, Nadia Boujelbene, Ahmed Baligh Laaribi, Nour Zidi, Mehdi Kehila, Hanène Chelbi, Abdellatif Boudabous, Karima Mrad, Amel Mezlini, Dario Di Luca, Hadda-Imene Ouzari, Roberta Rizzo, Inès Zidi
Human Leukocyte Antigen-G (HLA-G) is known as an immune suppressive molecule; it interacts with several immune cells and inhibits their functions. HLA-G molecule is highly represented in pathological conditions including malignant transformation. To the best of our knowledge this is the first study that focuses on the expression of soluble HLA-G (sHLA-G) in endometrial cancer (EC). We aimed at exploring sHLA-G plasma levels and its prognostic value in EC. We examined total sHLA-G expression as well as the sHLA-G1 and HLA-G5 isoforms expression in plasma samples from 40 patients with EC and 45 healthy controls by a specific sandwich ELISA...
May 3, 2018: Molecular Immunology
Vera Chayeb, Sana Mahjoub, Hedia Zitouni, Hanene Jrah-Harzallah, Khadija Zouari, Rached Letaief, Touhami Mahjoub
BACKGROUND AND AIM: Colorectal cancer (CRC) is a worldwide leading cause of mortality. Genetic studies have associated single nucleotide polymorphisms in genes encoding microRNAs with CRC risk but results are mostly inconclusive across variable ethnicities. In this study, we investigated the association of hsa-mir-149 rs2292832 C/T, hsa-mir-146a rs2910164 G/C and hsa-mir-196a2 rs11614913 C/T and explored their roles in clinicopathological features of CRC progression in an Eastern Tunisian cohort...
April 28, 2018: Gene
Raihane Ben Lakhal, Sondos Hdiji, Sami Zriba, Amina Mokrani, Mohamed Adnen Laatiri, Yossra BenYoussef, Faten Ezzaier, Nabil Toumi, Salwa Ladeb, Hanen BenSalah, Sameh Tebra, Hatem Frikha, Taha Messai, Jamel Daoued, Noureddine Bouaouina, Monji Maalej, Mounir Frikha, Tarek BenOthmen, Slim BenAhmed, Abderrahim Khelif, Fehmi Msaddek, Amel Mezlini, Moez Elloumi, Balkis Meddeb
OBJECTIVE: In Tunisia, the management of Adult Hodgkin's Lymphoma (HL) has been standardized since 1999. We propose in this study to report the therapeutic results of the national protocol of adult HL treatment (MDH2008). PATIENTS AND METHODS: Our study is prospective multicenter interesting 444 patients followed for HL between July 2008 and June 2013 and treated according to the MDH2008 protocol. The median age of our patients was 30 years. B symptoms were present in 62...
April 27, 2018: Bulletin du Cancer
Taieb Ach, Hela Marmouch, Dorra Elguiche, Asma Achour, Hajer Marzouk, Hanene Sayadi, Ines Khochtali, Mondher Golli
Kallmann syndrome (KS) is a form of hypogonadotropic hypogonadism in combination with a defect in sense of smell, due to abnormal migration of gonadotropin-releasing hormone-producing neurons. We report a case of a 17-year-old Tunisian male who presented with eunuchoid body proportions, absence of facial, axillary and pubic hair, micropenis and surgically corrected cryptorchidism. Associated findings included anosmia. Karyotype was 46XY and hormonal measurement hypogonadotropic hypogonadism. MRI of the brain showed bilateral agenesis of the olfactory bulbs and 3...
2018: Endocrinology, Diabetes & Metabolism Case Reports
Hajer Ben Saad, Dorra Driss, Imen Jaballi, Hanen Ghozzi, Ons Boudawara, Michael Droguet, Christian Magné, Monsef Nasri, Khaled Mounir Zeghal, Ahmed Hakim, Ibtissem Ben Amara
OBJECTIVE: The current study aimed to elucidate the effect of vanillin on behavioral changes, oxidative stress, and histopathological changes induced by potassium bromate (KBrO3), an environmental pollutant, in the cerebellum of adult mice. METHODS: The animals were divided into four groups: group 1 served as a control, group 2 received KBrO3, group 3 received KBrO3 and vanillin, and group 4 received only vanillin. We then measured behavioral changes, oxidative stress, and molecular and histological changes in the cerebellum...
February 2018: Biomedical and Environmental Sciences: BES
Bassem Khemakhem, Slim Smaoui, Hanen El Abed, Imen Fendri, Hajer Hammami, Mohamed Ali Ayadi
Three different concentrations of a purified maltogenic amylase (FSA) from fenugreek ( Trigonella foenum graecum ) seeds were incorporated into the cake formulation. The addition of FSA at 0.003, 0.005 and 0.01 U/g of cake increased the loaf volume, the number of holes (gas cells), and water absorption. Textural study revealed an improvement of the cake quality, resulting in the decrease of hardness and the increase of cohesion. Environmental scanning electron microscopy was performed on different cakes to evaluate the influence of amylase activity on microstructure...
March 2018: 3 Biotech
Wafa Hassen, Mohamed Neifar, Hanene Cherif, Afef Najjari, Habib Chouchane, Rim C Driouich, Asma Salah, Fatma Naili, Amor Mosbah, Yasmine Souissi, Noura Raddadi, Hadda I Ouzari, Fabio Fava, Ameur Cherif
A number of Pseudomonas strains function as inoculants for biocontrol, biofertilization, and phytostimulation, avoiding the use of pesticides and chemical fertilizers. Here, we present a new metabolically versatile plant growth-promoting rhizobacterium, Pseudomonas rhizophila S211, isolated from a pesticide contaminated artichoke field that shows biofertilization, biocontrol and bioremediation potentialities. The S211 genome was sequenced, annotated and key genomic elements related to plant growth promotion and biosurfactant (BS) synthesis were elucidated...
2018: Frontiers in Microbiology
Najla El-Hachem, Nadia Habel, Tanesha Naiken, Hanene Bzioueche, Yann Cheli, Guillaume E Beranger, Emilie Jaune, Florian Rouaud, Nicolas Nottet, Frédéric Reinier, Céline Gaudel, Pascale Colosetti, Corine Bertolotto, Robert Ballotti
HACE1 is an E3 ubiquitin ligase described as a tumour suppressor because HACE1-knockout mice develop multi-organ, late-onset cancers and because HACE1 expression is lost in several neoplasms, such as Wilms' tumours and colorectal cancer. However, a search of public databases indicated that HACE1 expression is maintained in melanomas. We demonstrated that HACE1 promoted melanoma cell migration and adhesion in vitro and was required for mouse lung colonisation by melanoma cells in vivo. Transcriptomic analysis of HACE1-depleted melanoma cells revealed an inhibition of ITGAV and ITGB1 as well changes in other genes involved in cell migration...
March 7, 2018: Cell Death and Differentiation
Najla Mechergui, Nesrine Chaouech, Hanéne Ben Said, Mejda Bani, Radhia Boujday, Nizar Ladhari
INTRODUCTION: The evaluation of fitness for work can be conducted upon the employer's request even during the employee's sickness absence. AIM: Determine the contribution of conducting medical examinations to assess the working ability of Charles Nicolle hospital's workers who are on sick leave. METHODS: A descriptive and retrospective study included all the medical records, completed over a two-year period (2013-2014), of Charles Nicolle hospital's personnel who were on sick leave and who consulted the service of occupational medicine and professional pathology to undergo a fitness for work evaluation requested by the employer...
May 2017: La Tunisie Médicale
Hanen Ghazali, Rania Jebri, Anware Yahmadi, Najla El Heni, Ines Chermiti, Sami Souissi
BACKGROUND: The objective of our study was to evaluate the predictors of repeat visits at Emergency Department (ED) in patients aged ≥ 65 years. METHODS: A prospective observational study was conducted over a month. Inclusion:  patients (age> = 65 year) seen in the ED and leaving the same day. The epidemiological and clinical characteristics at the first visit (index visit (IV)) were collected. The Identification of Senior At Risk (ISAR) score was used to assess our patients...
May 2017: La Tunisie Médicale
Habib Mosbah, Hassiba Chahdoura, Jannet Kammoun, Malek Besbes Hlila, Hanen Louati, Saoussen Hammami, Guido Flamini, Lotfi Achour, Boulbaba Selmi
BACKGROUND: α-glucosidase is a therapeutic target for diabetes mellitus (DM) and α-glucosidase inhibitors play a vital role in the treatments for the disease. Furthermore, xanthine oxidase (XO) is a key enzyme that catalyzes hypoxanthine and xanthine to uric acid which at high levels can lead to hyperuricemia which is an important cause of gout. Pancreatic lipase (PL) secreted into the duodenum plays a key role in the digestion and absorption of fats. For its importance in lipid digestion, PL represents an attractive target for obesity prevention...
March 5, 2018: BMC Complementary and Alternative Medicine
Najeh Krayem, Goetz Parsiegla, Hélène Gaussier, Hanen Louati, Raida Jallouli, Pascal Mansuelle, Frédéric Carrière, Youssef Gargouri
BACKGROUND: Heterodimeric phospholipase A2 from venom glands of Tunisian scorpion Scorpio maurus (Sm-PLGV) had been purified. It contains long and short chains linked by a disulfide bridge. Sm-PLGV exhibits hemolytic activity towards human erythrocytes and interacts with phospholipid monolayers at high surface pressure. The investigation of structure-function relationships should provide new clues to understand its activity. METHODS: Molecular cloning of Sm-PLGV and heterologous expression in Escherichia coli of three recombinant forms was used to determine the role of the short chain on enzymatic activity...
May 2018: Biochimica et Biophysica Acta
Naima Boutaghane, Abdulmagid Alabdul Magid, Amin Abedini, Anaïs Cafolla, Hanène Djeghim, Sophie C Gangloff, Laurence Voutquenne-Nazabadioko, Zahia Kabouche
A previously undescribed triterpenoid saponin, 3-O-[α-l-rhamnopyranosyl-(1→2)-{β-d-glucopyranosyl-(1→6)-}β-d-galactopyranosyl-(1→2)-β-d-glucuronopyranosyl]-sophoradiol (1), in addition to twenty-nine known constituents (2-30) were isolated from the aerial parts of Genista numidica Spach. Structures elucidation was performed by comprehensive 1D- and 2D-NMR analyses and HRESIMS. The extracts, fractions and isolated compounds were evaluated for their antibacterial, antioxidant and tyrosinase inhibitory activities...
February 15, 2018: Natural Product Research
Hanen Boukedi, Slim Tounsi, Lobna Abdelkefi-Mesrati
The carob moth, Ectomyelois ceratoniae, is an important agricultural pest that is susceptible to the Vip3(459) protein. The insecticidal activity, evaluated against this lepidopteran pest, displayed an LC50 value of about 28 ng/cm2 . The investigation of the mode of action of this B. thuringiensis protein demonstrated that the active form of this toxin bound to putative receptors in the BBMV of E. ceratoniae. Ligand blotting experiment proved that Vip3(459) specifically bound to two proteins of about 53 and 57 kDa, located on the midgut...
June 2018: Acta Tropica
H Bouzidi, A Emirian, A Marty, E Chachaty, A Laplanche, B Gachot, F Blot
OBJECTIVES: Differential time to positivity of cultures of blood drawn simultaneously from central venous catheter and peripheral sites is widely used to diagnose catheter-related bloodstream infections without removing the catheter. However, the accuracy of this technique for some pathogens, such as Staphylococcus aureus, is debated in routine practice. METHODS: In a 320-bed reference cancer centre, the charts of patients with at least one blood culture positive for S...
February 10, 2018: Journal of Hospital Infection
Rabeb M Ghali, Maryam A Al-Mutawa, Abrar K Al-Ansari, Sonia Zaied, Hanen Bhiri, Touhami Mahjoub, Wassim Y Almawi
BACKGROUND: Estrogen is key to breast cancer pathogenesis, and acts by binding its receptor (ER), which exists as ERα and ERβ, encoded by ESR1 and ESR2 genes, respectively. Studies that investigated the association of ESR1 and ESR2 variants with breast cancer yielded mixed outcome, and ethnic contribution was proposed. We evaluated the association between ESR1 and ESR2 variants and breast cancer and associated features in Tunisian women. METHODS: Retrospective case-control study involving 207 female breast cancer patients, and 284 control women...
April 20, 2018: Gene
Mariam Siala, Markus Rihl, Hanen Sellami, Abir Znazen, Nadia Sassi, Lilia Laadhar, Radhouane Gdoura, Imen Belghuith, Dalila Mrabet, Sofien Baklouti, Slaheddine Sellami, Jean Sibilia, Hela Fourati, Adnene Hammami, Ilhem Cheour
Enterobacterial components in the joints of patients are believed to contribute to a perpetuating inflammation leading to a reactive arthritis (ReA), a condition in which microbial agents cannot be recovered from the joint. At present, it is unclear whether nucleic acids from Shigella spp. are playing a pathogenic role in causing not only ReA but also other forms of arthritis. Quantitative real-time polymerase chain reaction assay (qPCR) is the method of choice for the identification of bacteria within the synovium...
February 5, 2018: Rheumatology International
Mourad Ben Said, Hanène Belkahia, Lilia Messadi
The genus Anaplasma belonging to the Anaplasmataceae family (order Rickettsiales) comprises obligate intracellular Gram-negative bacteria of veterinary and public health importance. Six species and five types of strains genetically related are currently assigned to the genus Anaplasma including Anaplasma marginale, A. centrale, A. bovis, A. phagocytophilum, A. ovis and A. platys as classified species, and "A. capra", A. odocolei sp. nov., A. phagocytophilum-like 1 (Anaplasma sp.-Japan), A. phagocytophilum-like 2 (Anaplasma sp...
March 2018: Ticks and Tick-borne Diseases
Hanen Boukedi, Saoussen Ben Khedher, Lobna Abdelkefi-Mesrati, Jeroen Van Rie, Slim Tounsi
The cotton leaf worm Spodoptera littoralis is known for causing serious damages to various crops. In this study, the susceptibility/tolerance of this larvae to four Vip3A (Vip3Aa, Vip3Ae, Vip3Ad and Vip3Af) toxins was investigated. UnlikeVip3Ad which showed no activity to S. littoralis, Vip3Aa, Vip3Ae and Vip3Af exhibited high toxicity to this larva with LC50 of 228.42 ng/cm2 , 65.71 ng/cm2 , and 388.90 ng/cm2 , respectively. Activation of the 90 kDa Vip3A proteins by S. littoralis larvae juice generated four major bands of sizes 62, 45, 33 and 22 kDa...
February 2018: Journal of Invertebrate Pathology
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