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https://www.readbyqxmd.com/read/28728113/catalase-based-hydrogen-peroxide-biosensor-for-mercury-determination-by-inhibition-measurements
#1
Basant Elsebai, Mariana Emilia Ghica, Mohammed Nooredeen Abbas, Christopher M A Brett
A new amperometric hydrogen peroxide enzyme inhibition biosensor for the indirect determination of toxic mercury ions, Hg(2+), based on catalase immobilized on a glassy carbon electrode surface by cross-linking with glutaraldehyde and bovine serum albumin, is reported. The parameters influencing biosensor performance were optimized, including enzyme loading, the amount of hydrogen peroxide, the applied potential and electrolyte pH. It was shown that the inhibition of catalase by Hg(2+) species is irreversible, with a linear inhibition response between 5×10(-11) and 5×10(-10)M...
July 11, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28728044/atomic-force-microscopy-study-of-morphological-modifications-induced-by-different-decontamination-treatments-on-escherichia-coli
#2
Florina Lucica Zorila, Cristina Ionescu, Liviu Stefan Craciun, Bogdan Zorila
In this paper we used atomic force microscopy (AFM) to investigate the surface morphology of Escherichia coli, after being subjected to decontamination treatments, at sub-MICs levels (minimal inhibitory concentrations), with different disinfectants used in hospitals, pharmaceutical, food industry and even in our home, as an essential means to prevent the spreading of microorganisms. This article focuses on different morphological modifications adopted by E. coli cells as responses to the different modes of action of these substances...
July 14, 2017: Ultramicroscopy
https://www.readbyqxmd.com/read/28728007/direct-immune-detection-of-cortisol-by-chemiresistor-graphene-oxide-sensor
#3
Yo-Han Kim, Kyungmin Lee, Hunsang Jung, Hee Kyung Kang, Jihoon Jo, In-Kyu Park, Hyun Ho Lee
In this study, a biosensor to detect a stress biomarker of cortisol using cortisol monoclonal antibody (c-Mab) covalently immobilized on reduced graphene oxide (rGO) channel as electrical sensing element was demonstrated. Highly specific immune-recognition between the c-Mab and the cortisol was identified and characterized on a basis of resistance change at the rGO channel based chemiresistor sensor achieving the limit of detection of 10pg/mL (27.6 pM). In addition, cortisol concentrations of real human salivary sample and buffer solution of rat adrenal gland acute slices, which could secret the cortisol induced by adrenocorticotropic hormone (ACTH), were directly measured by the chemiresistor corresponding to the specific sensing of the cortisol...
July 11, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28727916/transient-catalytic-voltammetry-of-sulfite-oxidase-reveals-rate-limiting-conformational-changes
#4
Ting Zeng, Silke Leimkühler, Ulla Wollenberger, Vincent Fourmond
Sulfite oxidases are metalloenzymes that oxidize sulfite to sulfate at a molybdenum active site. In vertebrate sulfite oxidases, the electrons generated at the Mo center are transferred to an external electron acceptor via a heme domain, which can adopt two conformations: a "closed" conformation, suitable for internal electron transfer, and an "open" conformation suitable for intermolecular electron transfer. This conformational change is an integral part of the catalytic cycle. Sulfite oxidases have been wired to electrode surfaces, but their immobilization lead to a significant decrease in their catalytic activity, raising the question of the occurrence of the conformational change when the enzyme is on an electrode...
July 20, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28727913/alpha-l-fucosidase-immunoassay-for-early-detection-of-hepatocellular-carcinoma
#5
Eric Waidely, Abdulrahman Obaid Al-Youbi, Abdulaziz Saleh Omar Bashammakh, Mohammad Soror El-Shahawi, Roger M Leblanc
Detection of alpha-L-fucosidase has been shown to have relevance in diagnosing hepatocellular carcinoma. Few assays have been developed to measure this enzyme, with most relying on colorimetric techniques involving the enzyme's kinetics. While these assays are facile and quick, the sensitivity is not always sufficient for early tumor detection. To improve upon previous assays for alpha-L-fucosidase, a fluorescence based immunoassay was produced implementing an alpha-L-fucosidase specific antibody (FUCA2). The immobilization of the alpha-L-fucosidase-specific antibody onto a quartz slide was investigated with several bioconjugation approaches and an immunoassay for detection of alpha-L-fucosidase was produced...
July 20, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28727476/options-for-addressing-exocrine-pancreatic-insufficiency-in-patients-receiving-enteral-nutrition-supplementation
#6
Steven D Freedman
Patients with exocrine pancreatic insufficiency (EPI) have suboptimal secretion of pancreatic digestive enzymes and experience a range of clinical symptoms related to the malabsorption of fat. In patients with EPI unable to meet their nutritional requirements, enteral nutrition (EN) support is used to augment nutritional status. In addition to protein and carbohydrate, EN formulas contain fats as a calorie source, as well as vitamins and minerals to help prevent nutritional deficiencies related to malabsorption...
July 2017: American Journal of Managed Care
https://www.readbyqxmd.com/read/28727264/phosphorylated-polyacrylonitrile-fiber-as-an-efficient-and-greener-acetalization-catalyst
#7
Gang Xu, Jian Cao, Yali Zhao, Lishuo Zheng, Minli Tao, Wenqin Zhang
A novel solid acid catalyst (PANEAPF) was developed by immobilization of phosphoric acid on polyacrylonitrile fiber via covalent bond. Various characterization techniques such as elemental analysis (EA), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR), etc. were utilized to confirm the successful grafting and the stability of the fiber catalysts during application. The PANEAPF showed high catalytic activity in the acetalization of aldehydes due to the high utilization efficiency of its functionalized acid sites...
July 20, 2017: Chemistry, An Asian Journal
https://www.readbyqxmd.com/read/28726727/in-vitro-quantified-determination-of-%C3%AE-amyloid-42-peptides-a-biomarker-of-neuro-degenerative-disorders-in-pbs-and-human-serum-using-a-simple-cost-effective-thin-gold-film-biosensor
#8
Yifan Dai, Alireza Molazemhosseini, Chung Chiun Liu
A simple in vitro biosensor for the detection of β-amyloid 42 in phosphate-buffered saline (PBS) and undiluted human serum was fabricated and tested based on our platform sensor technology. The bio-recognition mechanism of this biosensor was based on the effect of the interaction between antibody and antigen of β-amyloid 42 to the redox couple probe of K₄Fe(CN)₆ and K₃Fe(CN)₆. Differential pulse voltammetry (DPV) served as the transduction mechanism measuring the current output derived from the redox coupling reaction...
July 20, 2017: Biosensors
https://www.readbyqxmd.com/read/28726386/a-smart-biointerface-with-photoswitched-functions-between-bactericidal-activity-and-bacteria-releasing-ability
#9
Ting Wei, Wenjun Zhan, Qian Yu, Hong Chen
Smart biointerfaces with capability to regulate cell-surface interactions in response to external stimuli are of great interest for both fundamental research and practical applications. Smart surfaces with "ON/OFF" switchability for a single function such as cell attachment/detachment are well-known and useful, but the ability to switch between two different functions may be seen as the next level of "smart". In this work reported, a smart supramolecular surface capable of switching functions reversibly between bactericidal activity and bacteria-releasing ability in response to UV-visible light is developed...
July 20, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28726323/characterization-of-a-cathepsin-d-protease-from-cho-cell-free-medium-and-mitigation-of-its-impact-on-the-stability-of-a-recombinant-therapeutic-protein
#10
Amareth Lim, Brandon L Doyle, Gerard M Kelly, Angelia M Reed-Bogan, Lawrence H Breen, Parviz A Shamlou, Peter K Lambooy
During purification process development of a recombinant therapeutic protein, an endoproteolytic activity endogenous to the Chinese hamster ovary (CHO) cells and leading to degradation at particular hydrophobic amino acid residues (e.g., Phe and Trp) was observed when processing at acidic pH. The presence of residual levels of protease activity in purified protein batches affected the inherent activity of the product when stored as a solution. In order to develop a robust purification strategy to minimize this undesirable impact, identification and characterization of this protease was essential to ultimately ensure that a solution formulation was stable for many years...
July 20, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/28726220/effect-of-operational-factors-on-bioregeneration-of-binary-phenol-and-4-chlorophenol-loaded-granular-activated-carbon-using-pva-immobilized-biomass-cryogels
#11
Kwok-Yii Leong, Rohana Adnan, Poh-Eng Lim, Si-Ling Ng, Chye-Eng Seng
The effects of dry biomass density in cryogel beads, shaking speed and initial concentration ratio of phenol to 4-chlorophenol (4-CP) on the bioregeneration efficiencies of binary phenol and 4-CP-loaded granular activated carbon (GAC) for phenol and 4-CP, respectively, were investigated under the simultaneous adsorption and biodegradation approach. The results revealed higher bioregeneration efficiencies of binary-loaded GAC for phenol and 4-CP at higher dry biomass density but moderate shaking speed. The optimum dry biomass density in cryogel beads and shaking speed for use in bioregeneration were found to be 0...
July 18, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28726197/effect-of-phenibut-and-glufimet-a-novel-glutamic-acid-derivative-on-respiration-of-heart-and-brain-mitochondria-from-animals-exposed-to-stress-against-the-background-of-inducible-no-synthase-blockade
#12
V N Perfilova, T A Popova, I I Prokofiev, I S Mokrousov, O V Ostrovskii, I N Tyurenkov
Increased oxygen consumption by heart and brain mitochondria in the absence of ADP and reduced mitochondrial respiration in the presence of ADP were observed in rats exposed to stress simulated by suspension by the dorsal neck skin fold for 24 h, which attests to uncoupling of substrate oxidation and ATP synthesis and can cause electron drain from the respiratory chain, formation of ROS, and oxidative damage to cell structures. Blockade of inducible NO synthase with aminoguanidine (single intraperitoneal dose of 50 mg/kg before stress exposure) increased coupling of respiration and oxidative phosphorylation in heart and brain mitochondria of rats exposed to immobilization-painful stress, which was especially pronounced in cardiomyocytes...
July 18, 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28726102/stereoselective-biotransformation-of-phenylglycine-nitrile-by-heterogeneous-biocatalyst-based-on-immobilized-bacterial-cells-and-enzyme-preparation
#13
Yu G Maksimova, A N Gorbunova, V A Demakov
We studied the effect of a heterogeneous environment on the stereoselectivity of transformation of racemic phenylglycine nitrile. Immobilized biocatalysts were prepared by adhesion of Pseudomonas fluorescens C2 cells on carbon-containing supports and covalent crosslinking of nitrile hydratase and amidase of Rhodococcus rhodochrous 4-1 to activated chitosan as well as by the method of cross-linked aggregates. At a reaction duration of 20 h, the ratio of phenylglycine stereoisomers changes depending on the presence of support in medium...
May 2017: Doklady. Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28726053/the-effect-of-different-ankle-and-knee-supports-on-balance-in-early-ambulation-of-post-stroke-hemiplegic-patients
#14
Burcu Talu, Zilan Bazancir
The purpose of this study was to compare the effects of different ankle and knee supports on balance in early ambulation of post-stroke hemiplegic patients. This is a randomized experimental study. The study sample included 20 hemiplegic patients who were able to stand with support and who had been diagnosed with a cerebrovascular accident within the last month. A knee immobilization brace (KIB) was first placed on each individual (first application), followed by placement of knee immobilizer brace and Foot Lifter Orthosis ® (FLO) (second application), and lastly, placement of KIB and rigid taping (RT) (third application)...
July 19, 2017: Neurological Sciences
https://www.readbyqxmd.com/read/28725573/determination-of-nitrogen-balance-in-agroecosystems
#15
Upendra M Sainju
Nitrogen balance in agroecosystems provides a quantitative framework of N inputs and outputs and retention in the soil that examines the sustainability of agricultural productivity and soil and environmental quality. Nitrogen inputs include N additions from manures and fertilizers, atmospheric depositions including wet and dry depositions, irrigation water, and biological N fixation. Nitrogen outputs include N removal in crop grain and biomass and N losses through leaching, denitrification, volatilization, surface runoff, erosion, gas emissions, and plant senescence...
2017: MethodsX
https://www.readbyqxmd.com/read/28725501/functional-outcomes-of-24-hour-thumb-immobilization-in-healthy-volunteers-prospective-comparative-study
#16
Raghuveer C Muppavarapu, Lyubov Tsytsikova, Michael B Gottschalk, Poonam P Dalwadi, Charles Cassidy
Background  Immobilization is often needed for the treatment of wrist and hand injuries. The current best method of immobilization for several types of injuries has yet to be elucidated with little being reported on the functional differences of each type of immobilization. Purpose  The purpose of this study is to compare the functional outcome between healthy young volunteers with a 24-hour short arm cast (SAC) versus thumb spica cast (TSC) immobilization. Methods  A total of 50 healthy volunteers completed a baseline typing assessment and a Patient-Reported Outcomes Measurement Information System (PROMIS) upper extremity functional scoring assessment...
August 2017: Journal of Wrist Surgery
https://www.readbyqxmd.com/read/28725015/all-printed-highly-sensitive-2d-mos2-based-multi-reagent-immunosensor-for-smartphone-based-point-of-care-diagnosis
#17
Memoon Sajid, Ahmed Osman, Ghayas Uddin Siddiqui, Hyun Bum Kim, Soo Wan Kim, Jeong Bum Ko, Yoon Kyu Lim, Kyung Hyun Choi
Immunosensors are used to detect the presence of certain bio-reagents mostly targeted at the diagnosis of a condition or a disease. Here, a general purpose electrical immunosensor has been fabricated for the quantitative detection of multiple bio-reagents through the formation of an antibody-antigen pair. The sensors were fabricated using all printing approaches. 2D transition metal dichalcogenide (TMDC) MoS2 thin film was deposited using Electrohydrodynamic atomization (EHDA) on top of an interdigitated transducer (IDT) electrode fabricated by reverse offset printing...
July 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28723840/cervical-spine-evaluation-and-clearance-in-the-intoxicated-patient-a-prospective-western-trauma-association-multi-institutional-trial-and-survey
#18
Matthew J Martin, Lisa D Bush, Kenji Inaba, Saskya Byerly, Martin Schreiber, Kimberly A Peck, Galinos Barmparas, Jay Menaker, Joshua P Hazelton, Raul Coimbra, Martin D Zielinski, Carlos V R Brown, Chad G Ball, Jill R Cherry-Bukowiec, Clay Cothren Burlew, Julie Dunn, C Todd Minshall, Matthew M Carrick, Gina M Berg, Demetrios Demetriades, William Long
INTRODUCTION: Intoxication often prevents clinical clearance of the cervical spine (Csp) after trauma leading to prolonged immobilization even with a normal CT scan. We evaluated the accuracy of CT at detecting clinically significant Csp injury, and surveyed participants on related opinions and practice. METHODS: A prospective multicenter study (2013-2015) at 17 centers. All adult blunt trauma patients underwent structured clinical examination and imaging including a Csp CT, with follow-up thru discharge...
July 19, 2017: Journal of Trauma and Acute Care Surgery
https://www.readbyqxmd.com/read/28723346/co-immobilization-of-pd-and-zn-nanoparticles-in-chitosan-silica-membranes-for-efficient-recyclable-catalysts-used-in-ullmann-reaction
#19
Qi Liu, Mengdie Xu, Yudong Wang, Ruokun Feng, Zhen Yang, Shufeng Zuo, Chenze Qi, Minfeng Zeng
In this study, an efficient heterogeneous catalyst including palladium (Pd) and zinc (Zn) nanoparticles supported on chitosan/silica (CS/SiO2) composite membrane is synthesized using partially etching of SiO2 technique. N2 sorption isotherm results shows that the prepared Pd-Zn@CS/SiO2 (1/1) porous membrane had a BET surface area of 26.50m(2)/g. High-resolution transmission electron microscopy (HR-TEM) and X-ray photoelectron spectroscopy (XPS), characterization of the catalyst shows that the Pd(00) nanoparticles (below 5nm), and Zn(00) aggregates (about 10-15nm) dispersed well in the CS/SiO2 matrix...
July 16, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28723345/%C3%AE-glucosidase-immobilization-on-chitosan-enriched-magnetic-composites-for-enzyme-inhibitors-screening
#20
Dong-Mei Liu, Juan Chen, Yan-Ping Shi
In the present study, the chitosan-enriched magnetic composites (MCCs) were prepared by a novel and simple embedding method for the immobilization of α-glucosidase (α-Glu). The immobilized α-Glu could be easily separated from the reaction mixture under an external magnetic field owing to the magnetic support. With the MCCs-immobilized α-Glu, enzyme activity and stability were studied, and enzyme inhibitors were screened from traditional Chinese medicines (TCMs) and vegetables combined with capillary electrophoresis (CE)...
July 16, 2017: International Journal of Biological Macromolecules
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