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https://www.readbyqxmd.com/read/29149729/functionalized-gold-nanoparticles-as-affinity-nanoprobes-for-multiple-lectins
#1
Karuppuchamy Selvaprakash, Yu-Chie Chen
Glycan-lectin interactions are commonly observed in nature. Analytical methods, which are used to detect lectins that rely on the use of glycan ligand-modified nanoprobes as affinity probes, have been developed. Most of the existing methods are focused on the use of synthetic glycan ligands. Nevertheless, naturally available glycoproteins, such as ovalbumin in chicken egg whites, are good sources for fabricating glycan-immobilized nanoprobes. In this study, we generated functionalized gold nanoparticles (Au NPs) from a one-pot reaction by reacting chicken egg white (cew) proteins with aqueous tetrachloroaurate...
November 8, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29149722/effects-of-middle-ear-quasi-static-stiffness-on-sound-transmission-quantified-by-a-novel-3-axis-optical-force-sensor
#2
Ivo Dobrev, Jae Hoon Sim, Baktash Aqtashi, Alexander M Huber, Thomas Linder, Christof Röösli
BACKGROUND: Intra-operative quantification of the ossicle mobility could provide valuable feedback for the current status of the patient's conductive hearing. However, current methods for evaluation of middle ear mobility are mostly limited to the surgeon's subjective impression through manual palpation of the ossicles. This study investigates how middle ear transfer function is affected by stapes quasi-static stiffness of the ossicular chain. The stiffness of the middle ear is induced by a) using a novel fiber-optic 3-axis force sensor to quantify the quasi-static stiffness of the middle ear, and b) by artificial reduction of stapes mobility due to drying of the middle ear...
November 10, 2017: Hearing Research
https://www.readbyqxmd.com/read/29149687/pyrrolidinyl-pna-polypyrrole-silver-nanofoam-electrode-as-a-novel-label-free-electrochemical-mirna-21-biosensor
#3
Tawatchai Kangkamano, Apon Numnuam, Warakorn Limbut, Proespichaya Kanatharana, Tirayut Vilaivan, Panote Thavarungkul
A label-free electrochemical miRNA biosensor was developed based on a pyrrolidinyl peptide nucleic acid (acpcPNA)/polypyrrole (PPy)/silver nanofoam (AgNF) modified electrode. The AgNF was electrodeposited as redox indicator on a gold electrode, which was then functionalized with an electropolymerized layer of PPy, a conducting polymer, to immobilize the PNA probes. The fabrication process was investigated by electrochemical impedance spectroscopy. The biosensor was used to detect miRNA-21, a biomarker abnormally expressed in most cancers...
November 6, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29149555/mapping-the-reactions-in-a-single-zero-valent-iron-nanoparticle
#4
Lan Ling, Xiao-Yue Huang, Meirong Li, Wei-Xian Zhang
Nanoscale zero-valent iron (nZVI) posseses unique functionalities for metal-metalloid removal and sequestration. So far, direct evidence on the heavy metal-iron reactions in the solid phase is still limited due to low concentration of heavy metals and small size of nanoparticles. In this work, angstrom-resolution spectral mappings on the reactions of nZVI with chromate, arsenate, nickel, silver, cesium, and zinc ions are presented. This work was achieved with spherical aberration-corrected scanning transmission electron microscopy integrated with high-sensitivity X-ray energy-dispersive spectroscopy scanning transmission electron microscopy (XEDS-STEM)...
November 17, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/29148888/tissue-residue-levels-after-immobilization-of-rocky-mountain-elk-cervus-elaphus-nelsoni-using-a-combination-of-nalbuphine-medetomidine-and-azaperone-antagonized-with-naltrexone-atipamezole-and-tolazoline
#5
Lisa L Wolfe, Pauline Nol, Matthew P McCollum, Travis Mays, Morgan E Wehtje, William R Lance, Mark C Fisher, Michael W Miller
Previous studies demonstrated that nalbuphine, medetomidine, and azaperone (NalMed-A) can effectively immobilize adult elk (Cervus elaphus nelsoni), and be antagonized using naltrexone and atipamezole, with or without tolazoline. To assess duration of tissue residues for this immobilization package, we immobilized 14 captive adult elk with NalMed-A, then euthanized animals and collected tissues 0, 3, 6, 14, 21, or 28 d later. Except for two animals euthanized immediately, all elk were recovered using naltrexone, atipamezole, and tolazoline...
November 17, 2017: Journal of Wildlife Diseases
https://www.readbyqxmd.com/read/29148699/controlling-ligand-spacing-on-surface-polyproline-based-fluorous-microarray-as-a-tool-in-spatial-specificity-analysis-and-inhibitor-development-for-carbohydrate-protein-interactions
#6
Tse-Hsueh Lin, Cin-Hao Lin, Ying-Jie Liu, Chun Yi Huang, Yen-Cheng Lin, Sheng-Kai Wang
Multivalent carbohydrate-protein interactions are essential for many biological processes. Convenient characterization for multivalent binding property of proteins will aid the development of molecules to manipulate these processes. We exploited the polyproline helix II (PPII) structure as molecular scaffolds to adjust the distances between glycan ligand attachment sites at 9, 18, and 27 Å on a peptide scaffold. Optimized fluorous groups were also introduced to the peptide scaffold for immobilization to the microarray surface through fluorous interaction to control the orientation of the helical scaffolds...
November 17, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29148558/ultrasound-propelled-nanowire-motors-enhance-asparaginase-enzymatic-activity-against-cancer-cells
#7
Murat Uygun, Beatriz Jurado-Sánchez, Deniz Aktas Uygun, Virendra Vikram Singh, Liangfang Zhang, Joseph Wang
Ultrasound-(US) propelled nanowires consisting of Au/Ni/Au/PEDOT-PPy-COOH segments are modified with asparaginase enzyme and applied as an effective anti-cancer agent. After immobilization of asparaginase onto the surface of the nanowire motors, the enzyme displays enhanced thermal and pH stabilities, improved resistance towards protease, and higher affinity for the substrate. The fast motion of the motor-carrying asparaginase leads to greatly accelerated biocatalytic depletion of asparagine and hence to a significantly enhanced inhibition efficacy against El4 lymphoma cancer cells (92%) as compared to free enzyme counterpart (17%) and other control groups...
November 17, 2017: Nanoscale
https://www.readbyqxmd.com/read/29147828/a-reliable-spectral-method-to-reaction-diffusion-equations-in-entrapped-cell-photobioreactor-packed-with-gel-granules-using-chebyshev-wavelets
#8
M Salai Mathi Selvi, G Hariharan, K Kannan
A mathematical model of a reaction-diffusion within an entrapped-cell photobioreactor packed with gel granules containing immobilized photosynthetic bacterial cells is discussed. A theoretical model is based on a system of coupled nonlinear reaction-diffusion equations. In this research work, we have developed an efficient wavelet-based spectral approach for solving the proposed model. Analytical expressions for the concentration of substrate and product are established for all values of reaction-diffusion parameters using second kind Chebyshev wavelet method...
November 17, 2017: Journal of Membrane Biology
https://www.readbyqxmd.com/read/29147771/maximum-striking-velocities-in-strikes-with-steel-rods-the-influence-of-rod-length-rod-mass-and-volunteer-parameters
#9
T X Trinh, S Heinke, C Rode, S Schenkl, M Hubig, G Mall, Holger Muggenthaler
In blunt force trauma to the head caused by attacks with blunt instruments, contact forces can be estimated based on the conservation of momentum if impact velocities are known. The aims of this work were to measure maximum striking velocities and to examine the influence of rod parameters such as rod mass and length as well as volunteer parameters such as sex, age, body height, body mass, body mass index and the average amount of physical exercise. Steel rods with masses of 500, 1000 and 1500 g as well as lengths of 40, 65 and 90 cm were exemplarily tested as blunt instruments...
November 16, 2017: International Journal of Legal Medicine
https://www.readbyqxmd.com/read/29147710/step-by-step-build-up-of-covalent-poly-ethylene-oxide-nanogel-films
#10
S Zahouani, L Hurman, M De Giorgi, C Vigier-Carrière, F Boulmedais, B Senger, B Frisch, P Schaaf, P Lavalle, L Jierry
Hydrogels based on poly(ethylene glycol) (PEG) are commonly used for studies related to cell fate and tissue engineering. Here we present a new covalent layer-by-layer build-up process leading to PEG coatings of nanometer size called "nanogel films". Compared to macroscopic hydrogels, such nanogels should provide a fine control over the structure and the thickness of the coating. Alternated deposition of bifunctional and tetra functional PEG molecules reacting through thiol/maleimide click chemistry is evaluated by quartz crystal microbalance...
November 17, 2017: Nanoscale
https://www.readbyqxmd.com/read/29146968/heterologous-expression-and-characterization-of-a-new-lipase-from-pseudomonas-fluorescens-pf0-1-and-used-for-biodiesel-production
#11
Wu Liu, Menggang Li, Yunjun Yan
As a kind of important biocatalysts, Pseudomonas lipases are commonly applied in various industrial fields. Pflip1, a new extracellular lipase gene from Pseudomonas. fluorescens Pf0-1, was first cloned and respectively expressed in Escherichia coli BL21(DE3) and Pichia pastoris KM71, the recombinant proteins Pflip1a and Pflip1b were later purified separately. Then Pflip1a was further characterized. The optimum pH of Pflip1a was 8.0 and its optimal temperature was 70 °C. After incubation at 70 °C for 12 h, Pflip1a could retain over 95% of its original activity...
November 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29146192/calcium-imaging-method-to-visualize-the-spatial-patterns-of-neural-responses-in-the-pygmy-squid-idiosepius-paradoxus-central-nervous-system
#12
Motoki Koizumi, Shuichi Shigeno, Makoto Mizunami, Nobuaki K Tanaka
BACKGROUND: Cephalopods exhibit unique behaviors such as camouflage and tactile learning. The brain functions correlated to these behaviors have long been analyzed through behavioral observations of animals subject to surgical manipulation or electrical stimulation of brain lobes. However, physiological methods have rarely been introduced to investigate the functions of each individual lobe, though physiological work on giant axons and slices of the vertical lobe system of the cephalopods have provided deep insights into ion conductance of nerves and long-term synaptic plasticity...
November 13, 2017: Journal of Neuroscience Methods
https://www.readbyqxmd.com/read/29145857/treatment-of-charcot-neuroarthropathy-and-osteomyelitis-of-the-same-foot-a-retrospective-cohort-study
#13
Martin Berli, Lazaros Vlachopoulos, Sabra Leupi, Thomas Böni, Charlotte Baltin
BACKGROUND: We evaluated treatment of osteomyelitis in the foot in the presence of Charcot neuroarthropathy, a devastating condition with progressive degeneration and joint destruction. We hypothesized that there was a difference in (1) amputation rate, (2) amputation level, (3) duration of antibiotic therapy, and (4) duration of immobilization for treatment of osteomyelitis within versus outside the Charcot zone. METHODS: Forty patients (43 ft) diagnosed with Charcot neuroarthropathy and osteomyelitis of the same foot were retrospectively analyzed...
November 16, 2017: BMC Musculoskeletal Disorders
https://www.readbyqxmd.com/read/29145245/case-reports-about-an-overlooked-cause-of-neck-pain-calcific-tendinitis-of-the-longus-colli-case-reports
#14
Yeong-Jin Kim, Jae-Young Park, Ki-Young Choi, Bong-Ju Moon, Jung-Kil Lee
STUDY DESIGN: Case description. OBJECTIVE: Acute calcific tendinitis of the longus colli muscle is a rare clinical entity that causes severe neck pain. This entity is not well recognized due to its nonspecific presentation such as acute neck pain, neck stiffness, and odynophagia. The importance of this disease with a review of the literature is presented. SUMMARY OF BACKGROUND DATA: Acute calcific tendinitis of the longus colli muscle is an inflammatory condition caused by deposition of calcium hydroxyapatite in the superior oblique tendon fibers of the longus colli muscle...
November 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/29145180/misoplegia
#15
Montserrat G Delgado, Julien Bogousslavsky
In 1974, Critchley described misoplegia as the phenomenon in which a hemiplegic patient develops a morbid dislike towards the offending immobile limbs. Patients with misoplegia may employ, but more commonly strike their paretic limbs not recognized as self. The pathophysiological mechanism is not well understood. The handful of cases of misoplegia described in the literature, frequently presented a right hemispheric damage. However, patients with chronic spinal cord injury may also present this symptomatology...
2018: Frontiers of Neurology and Neuroscience
https://www.readbyqxmd.com/read/29145139/application-and-microbial-ecology-of-psychrotrophs-in-domestic-wastewater-treatment-at-low-temperature
#16
Zhenzhen Xu, Yue Ben, Zhonglin Chen, Anxi Jiang, Jimin Shen, Xiaoyun Han
The feasibility of a bunch of screened psychrotrophs being applied to low-temperature wastewater treatment was investigated. The screened psychrophillic strains are capable of growth at a broad temperature-range from 0 to 40 °C and exhibit a preferable TTC-dehydrogenase activity at low temperature (4-10 °C). Along the sharply fluctuant temperatures (25-4-25 °C), the screened psychrotrophs (compared with the indigenous mesophiles) demonstrate less fluctuations of COD removal and more rapid recovery after temperature shocks...
October 24, 2017: Chemosphere
https://www.readbyqxmd.com/read/29145133/simultaneous-treatment-of-dimethyl-disulfide-and-hydrogen-sulfide-in-an-alkaline-biotrickling-filter
#17
Luis Arellano-García, Sylvie Le Borgne, Sergio Revah
Foul odors comprise generally a complex mixture of molecules, where reduced sulfur compounds play a key role due to their toxicity and low odor threshold. Previous reports on treating mixtures of sulfur compounds in single biofilters showed that hydrogen sulfide (H2S) interferes with the removal and degradation of other sulfur compounds. In this study, hydrogen sulfide (H2S) and dimethyl disulfide (DMDS) were fed to an alkaline biotrickling filter (ABTF) at pH 10, to evaluate the simultaneous removal of inorganic and organic sulfur compounds in a single, basic-pH system...
October 17, 2017: Chemosphere
https://www.readbyqxmd.com/read/29145074/ratiometric-electrochemical-assay-for-sensitive-detecting-microrna-based-on-dual-amplification-mechanism-of-duplex-specific-nuclease-and-hybridization-chain-reaction
#18
Yan-Hong Yuan, Bao-Zhu Chi, Shao-Hua Wen, Ru-Ping Liang, Zhi-Mei Li, Jian-Ding Qiu
We propose a ratiometric electrochemical assay for detecting microRNA (miRNA) on the basis of dual-amplification mechanism by using distinguishable electrochemical signals from thionine (Thi) and ferrocene (Fc). The thiol-modified and ferrocene-labeled hairpin capture probes (CP) are first immobilized on an Au electrode via Au-S reaction. The target miRNA hybridizes with CP and unfolding the hairpin structure of CP to form miRNA-DNA duplexes. Then, kamchatka crab duplex specific nuclease (DSN) specifically cleaves the DNA in miRNA-DNA duplexes, leading to the release of miRNA and another cleaves cycle, meanwhile, numerous Fc leaves away from the electrode surface and leads to the signal-off of Fc...
November 8, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29145025/coupling-of-on-column-trypsin-digestion-peptide-mapping-and-principal-component-analysis-for-stability-and-biosimilarity-assessment-of-recombinant-human-growth-hormone
#19
Sara M Shatat, Basma M Eltanany, Abeer A Mohamed, Medhat A Al-Ghobashy, Faten A Fathalla, Samah S Abbas
Peptide mapping (PM) is a vital technique in biopharmaceutical industry. The fingerprint obtained helps to qualitatively confirm host stability as well as verify primary structure, purity and integrity of the target protein. Yet, in-solution digestion followed by tandem mass spectrometry is not suitable as a routine quality control test. It is time consuming and requires sophisticated, expensive instruments and highly skilled operators. In an attempt to enhance the fuctionality of PM and extract multi-dimentional data about various critical quality attributes and comparability of biosimilars, coupling of PM generated using immobilized trypsin followed by HPLC-UV to principal component analysis (PCA) is proposed...
November 11, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29144589/-sphingosine-1-phosphate-immobilized-on-nanotopographical-scaffolds-improve-myogenic-differentiation
#20
Min Suk Lee, Hee Seok Yang
The skeletal muscle consists of highly aligned dense cables of collagen fibers with nanometer feature size to support muscle fibers. The skeletal myocyte can be greatly affected to differentiate by their surrounding topographical structure. To improve myogenic differentiation, we fabricated cell culture platform that sphingosine-1-phosphate (S1P) which regulated myocyte behavior was immobilized on a biomimetic nanopatterned polyurethaneacrylate (PUA) substrate using 3,4-dihydroxyphenylalanine (L-DOPA) for providing topographical and biological cues synergistically...
November 16, 2017: Biotechnology Journal
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