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https://www.readbyqxmd.com/read/28538815/effects-of-electroacupuncture-on-stress-and-anxiety-related-responses-in-rats
#1
Ricardo M Bassetto, Tatiana Wscieklica, Kathlein C P Pouza, Daniela Ortolani, Milena B Viana, Isabel C Cespedes, Regina C Spadari
The aim of this work was to investigate if eletroacupuncture at PC6 would modulate the stress-induced anxiety-like behavior and the level of activation of several brain areas. Rats were distributed in groups: control; submitted to immobilization; submitted to immobilization and eletroacupuncture at PC6 or at the tail. Immobilization increased grooming and decreased stretched attend postures and the time spent in the open arms of the ele-vated plus-maze. Eletroacupuncture at PC6 or tail canceled the effect of immobilization on grooming and attenuated the stretched attend posture...
May 22, 2017: Anais da Academia Brasileira de Ciências
https://www.readbyqxmd.com/read/28538659/the-effects-of-dithiothreitol-on-dna
#2
Søren Fjelstrup, Marie Bech Andersen, Jonas Thomsen, Jing Wang, Magnus Stougaard, Finn Skou Pedersen, Yi-Ping Ho, Marianne Smedegaard Hede, Birgitta Ruth Knudsen
With the novel possibilities for detecting molecules of interest with extreme sensitivity also comes the risk of encountering hitherto negligible sources of error. In life science, such sources of error might be the broad variety of additives such as dithiothreitol (DTT) used to preserve enzyme stability during in vitro reactions. Using two different assays that can sense strand interruptions in double stranded DNA, we here show that DTT is able to introduce nicks in the DNA backbone. DTT was furthermore shown to facilitate the immobilization of fluorescent DNA on an NHS-ester functionalized glass surface...
May 24, 2017: Sensors
https://www.readbyqxmd.com/read/28538656/recent-advances-in-c-c-and-c-n-bond-forming-reactions-catalysed-by-polystyrene-supported-copper-complexes
#3
REVIEW
Pavel Drabina, Jan Svoboda, Miloš Sedlák
This present mini-review covers recently published results on Cu(I) and Cu(II) complexes immobilized on polystyrene carriers, which are used as heterogeneous, eco-friendly reusable catalysts applied for carbon-carbon and carbon-nitrogen forming reactions. Recent advances and trends in this area are demonstrated in the examples of oxidative homocoupling of terminal alkynes, the synthesis of propargylamines, nitroaldolization reactions, azide alkyne cycloaddition, N-arylation of nitrogen containing compounds, aza-Michael additions, asymmetric Friedel-Crafts reactions, asymmetric Mukaiyama aldol reactions, and asymmetric 1,3-dipolar cycloaddition of azomethine ylides...
May 24, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28538202/development-of-nanobiocatalysts-through-the-immobilization-of-pseudomonas-fluorescens-lipase-for-applications-in-efficient-kinetic-resolution-of-racemic-compounds
#4
Bharat P Dwivedee, Jayeeta Bhaumik, Shushil K Rai, Joydev K Laha, Uttam C Banerjee
The present work reports covalent immobilization of Pseudomonas fluorescens lipase (PFL) on functionalized multiwalled carbon nanotubes (MWCNTs) as a nanobiocatalyst (NBC). This nanobiocatalyst facilitates efficient kinetic resolution of (RS)-1-phenylethanol into (S)-1-phenylethanol [C=49.7%, eep=99.5%, ees=98.1% and E value=191.4]. The immobilized preparation (MWCNTs-PFL) showed ten-fold increase in activity, thermal stability upto 80 °C and recyclability (8 cycles). MWCNTs-PFL nanobioconjugate demonstrated better stability and enhanced activity compared to covalently immobilized PFL on other matrices (silver nanoparticles, gold nanoparticles and chitosan beads) used for the study...
May 11, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28537751/using-neutron-reflectometry-to-characterize-antimicrobial-protein-surface-coatings
#5
Peter W Akers, Andrew J Dingley, Simon Swift, Andrew R J Nelson, Julie Martin, Duncan James McGillivray
Understanding the interaction of adsorbed or covalently immobilized proteins with solid substrates at the molecular level guides the successful design of functionalized surfaces used in biomedical applications. In this report, neutron reflectometry (NR) was used to characterize the structure of surface-attached antimicrobial protein films, with antimicrobial activity assessed using an adaption of the Japanese Industrial Standard Test JIS Z 2801. NR allowed parameters influencing bioactivity to be measured at nanometer resolution and for conclusions about structural characteristics relating to bioactivity to be drawn...
May 24, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28535481/graphene-oxide-enzyme-hybrid-nanoflowers-for-efficient-water-soluble-dye-removal
#6
Hui Li, Jingwei Hou, Linlin Duan, Chao Ji, Yatao Zhang, Vicki Chen
High efficient enzyme immobilization on carbon based conductive supports could provide wide applications in energy and environmental science. Here, we synthesized a 3D flower-like structured self-assembly hybrid nanocomposite with copper phosphate, laccase, graphite oxide (GO) and carbon nanotubes (CNTs) via a facile one-pot strategy under mild conditions. The prepared nanocomposite exhibited very high enzyme loading and improved laccase activity. During the formation of the nanocomposite, the copper phosphate-laccase petals were interwined by CNTs, and GO nanosheets were further coated on the petal surface...
May 11, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28535471/graphene-oxide-supported-carbon-nanofiber-like-network-derived-from-polyaniline-a-novel-composite-for-enhanced-glucose-oxidase-bioelectrode-performance
#7
Zepeng Kang, Kailong Jiao, Xinping Xu, Ruiyun Peng, Shuqiang Jiao, Zongqian Hu
A three-dimensional architecture of PANI@GO hybrid was synthesized via in-situ polymerization of aniline monomers on the surface of graphene oxide (GO) and carbonized at 1600°C. The SEM images showed that surfaces of planar GO were covered by a compact nanofiber-like polyaniline (PANI) layer which presented an interconnected network. Nanofiber-like PANI on the GO surface was mostly preserved and became the carbon nanofibers (CNFs) after carbonization. The TEM images showed that the carbonized PANI grew seamlessly on the GO surface and served as conductive "network" between interlayers of GO...
May 12, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28535444/passive-detection-of-pb-in-water-using-rock-phosphate-agarose-beads
#8
Harry M Edenborn, Bret H Howard, James I Sams, Dorothy J Vesper, Sherie L Edenborn
In this study, passive detectors for Pb were prepared by immobilizing powdered rock phosphate in agarose beads. Rock phosphate has been used to treat Pb-contaminated waters and soil by fixing the metal as an insoluble pyromorphite mineral. Under lab conditions, Pb was rapidly adsorbed from aqueous solution by the beads over time, consistent with the acidic dissolution of rock phosphate, the precipitation of pyromorphite within the pore space of the agarose gel matrix, and surface exchange reactions. Net accumulation of Pb occurred when beads were exposed to simulated periodic releases of Pb over time...
April 15, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28535433/a-highly-selective-surface-coating-for-enhanced-membrane-rejection-of-endocrine-disrupting-compounds-mechanistic-insights-and-implications
#9
Hao Guo, Yu Deng, Zhikan Yao, Zhe Yang, Jianqiang Wang, Chuner Lin, Tong Zhang, Baoku Zhu, Chuyang Y Tang
We designed a highly selective surface coating to achieve enhanced rejection of endocrine disrupting compounds (EDCs) by nanofiltration membranes. A commercial NF90 membrane was first coated with polydopamine (PDA) followed by in situ immobilization of silver nanoparticles (AgNPs). This PDA/AgNPs coating greatly improved EDC rejection at the expense of slight water permeability loss (4-10%). This improvement in rejection can be attributed to a combination of enhanced size exclusion and suppressed hydrophobic interaction...
May 18, 2017: Water Research
https://www.readbyqxmd.com/read/28534808/development-towards-compact-nitrocellulose-based-interferometric-biochips-for-dry-eye-mmp9-label-free-in-situ-diagnosis
#10
Beatriz Santamaría, María F Laguna, David López-Romero, Ana L Hernandez, Francisco J Sanza, Álvaro Lavín, Rafael Casquel, María V Maigler, Rocío L Espinosa, Miguel Holgado
A novel compact optical biochip based on a thin layer-sensing surface of nitrocellulose is used for in-situ label-free detection of metalloproteinase (MMP9) related to dry eye disease. In this article, a new integrated chip with different interferometric transducers layout with an optimal sensing surface is reported for the first time. We demonstrate that specific antibodies can be immobilized onto these transducers with a very low volume of sample and with good orientation. Many sensing transducers constitute the presented biochip in order to yield statistical data and stability in the acquired measurements...
May 19, 2017: Sensors
https://www.readbyqxmd.com/read/28534472/evidence-for-robots
#11
Ravikiran Shenoy, Dinesh Nathwani
Robots have been successfully used in commercial industry and have enabled humans to perform tasks which are repetitive, dangerous and requiring extreme force. Their role has evolved and now includes many aspects of surgery to improve safety and precision. Orthopaedic surgery is largely performed on bones which are rigid immobile structures which can easily be performed by robots with great precision. Robots have been designed for use in orthopaedic surgery including joint arthroplasty and spine surgery. Experimental studies have been published evaluating the role of robots in arthroscopy and trauma surgery...
2017: SICOT-J
https://www.readbyqxmd.com/read/28534112/decolorization-of-textile-dye-rb19-using-volcanic-rock-matrix-immobilized-bacillus-thuringiensis-cells-with-surface-displayed-laccase
#12
Juan Wan, Xiaowen Sun, Cheng Liu, Mengjun Tang, Lin Li, Hong Ni
A triplicate volcanic rock matrix-Bacillus thuringiensis-laccase WlacD (VRMs-Bt-WlacD) dye decolorization system was developed. WlacD was displayed on the B. thuringiensis MB174 cell surface to prepare a whole-cell laccase biocatalyst by using two repeat N-terminal domains of autolysin Mbg (Mbgn)2 as the anchoring motif. Immunofluorescence microscopic assays confirmed that the fusion protein (Mbgn)2-WlacD was anchored on the surface of the recombinant B. thuringiensis MB174. After optimization by a single factor test, L 9(3(4))-orthogonal test, Plackett-Burman test, steepest ascent method, and Box-Behnken response surface methodology, the whole-cell specific laccase activity of B...
June 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28533888/neuroprotective-effects-of-melatonin-administration-against-chronic-immobilization-stress-in-rats
#13
Asmaa Ms Gomaa, Heba M Galal, Amal T Abou-Elgait
Chronic stress can impair brain functions and play a well-known role in the development of stress-related disorders such as anxiety. Melatonin (Mel) is a neurohormone which regulate several physiological processes including mood and behavior. This experimental study was designed to evaluate the effect of Mel on chronic immobilization stress (CIS) for 6 weeks in rats and to elucidate its possible underlying mechanisms. Twenty-eight adult male Wistar albino rats were divided into four equal groups: the control group, the Mel-treated group which was injected daily with Mel (10 mg/kg/day; IP) for 6 weeks, the stressed group which was subjected to CIS protocol daily for 6 weeks, and the Mel-treated stressed group which was injected with Mel and concurrently exposed to CIS protocol for 6 weeks...
2017: International Journal of Physiology, Pathophysiology and Pharmacology
https://www.readbyqxmd.com/read/28533794/boron-supply-enhances-aluminum-tolerance-in-root-border-cells-of-pea-pisum-sativum-by-interacting-with-cell-wall-pectins
#14
Xue Wen Li, Jia You Liu, Jing Fang, Lin Tao, Ren Fang Shen, Ya Lin Li, Hong Dong Xiao, Ying Ming Feng, Hai Xiang Wen, Jia Hua Guan, Li Shu Wu, Yong Ming He, Heiner E Goldbach, Min Yu
Aluminum (Al) toxicity is the primary factor limiting crop growth in acidic soils. Boron (B) alleviates Al toxicity in plants, which is mainly considered to be due to the formation of Rhamnogalacturonan II-B (RGII-B) complexes, which helps to stabilize the cytoskeleton. It is unclear yet whether this is due to the increasing of net negative charges and/or further mechanisms. Kinetics of Al accumulation and adsorption were investigated using entire cells, cell wall and pectin of root border cells (RBCs) of pea (Pisum sativum), to reveal the mechanism of B in interacting with alkali-soluble and chelator-soluble pectin for an increased Al tolerance in RBCs...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28533626/nonhealing-progressive-stress-fractures-of-the-foot-in-a-13-year-old-basketball-player-is-vitamin-k-deficiency-a-risk-factor
#15
Meral Bayramoğlu, Nuray Ünlütürk
[Purpose] To report an adolescent male basketball player with nonhealing stress fractures of the foot and discuss the probable factors. [Subject and Methods] A 13-year-old basketball player presented with right foot pain. He had been playing basketball for three years and practicing 5 days/week. He denied any increase in daily training intensity. Magnetic resonance imaging confirmed stress fractures of the cuboid and cuneiform, with mild edema of the soft tissues between the tarsal bones and tenosynovitis of the flexor hallucis and flexor digitorum longi...
April 2017: Journal of Physical Therapy Science
https://www.readbyqxmd.com/read/28533032/design-and-synthesis-of-an-immobilized-metal-affinity-chromatography-and-metal-oxide-affinity-chromatography-hybrid-material-for-improved-phosphopeptide-enrichment
#16
Da-Song Yang, Xi-Ying Ding, Hong-Ping Min, Bo Li, Meng-Xiang Su, Miao-Miao Niu, Bin Di, Fang Yan
Reversible phosphorylation of proteins is one of the most important post-translational modifications, while the detection of phosphopeptides is difficult due to their low abundance and the signal suppression of nonphosphorylated peptides. Therefore, selective enrichment of phosphopeptides from highly complicated mixtures is vital for MS-based phosphoproteome analysis. Despite various strategies have been developed, there is no single method that is capable of providing full coverage of the whole phosphoproteome...
May 10, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28532684/quantitative-determination-of-testosterone-levels-with-biolayer-interferometry
#17
Hao Zhang, Wei Li, Hong Luo, Guangming Xiong, Yuanhua Yu
Natural and synthetic steroid hormones are widely spread in the environment and are considered as pollutants due to their endocrine activities, even at low concentrations, which are harmful to human health. To detect steroid hormones in the environment, a novel biosensor system was developed based on the principle of biolayer interferometry. Detection is based on changes in the interference pattern of white light reflected from the surface of an optical fiber with bound biomolecules. Monitoring interactions between molecules does not require radioactive, enzymatic, or fluorescent labels...
May 19, 2017: Chemico-biological Interactions
https://www.readbyqxmd.com/read/28532103/sensitive-impedimetric-dna-biosensor-based-on-nb-v-codoped-tio2-for-breast-cancer-susceptible-gene-detection
#18
Yu Wang, Xianwei Huang, He Li, Linlang Guo
In this paper, a novel (Nb,V) codoped TiO2 (NVTO) nanoparticle was synthesized, and a novel electrochemical DNA biosensor based on NVTO was first developed for detection of breast cancer susceptible gene detection. The proposed DNA biosensors were fabricated by immobilization of the probe DNA on a nanoporous CHIT-NVTO thin film deposited on an ITO-coated glass plate. The prepared NVTO composite film was characterized by XRD, SEM and FTIR. The results indicated that the synergistic effect of CHIT-NVTO nanocomposite can increase the electroactive surface area and the loading amounts of ssDNA...
August 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28532088/mussel-inspired-surface-modification-of-titania-nanotubes-as-a-novel-drug-delivery-system
#19
Negin Khoshnood, Ali Zamanian, Abouzar Massoudi
Titania nanotubes (TNTs) have attracted considerable attention for the development of new devices for local drug delivery applications. In this study TNTs were synthesized by hydrothermal method from titania nanoparticles and then the surface of TNTs were functionalized by in situ polymerization of bioinspired polydopamine (PDA). The proposed strategies emphasized on remarkable properties of these materials and their unique combination to design local drug delivery system with advanced performance. The samples were characterized using Transmission Electron Microscope (TEM), Field Emission Scanning Electron Microscope (FESEM), X-ray diffraction pattern (XRD), Fourier-transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA) and surface area analysis (BET)...
August 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28532080/antibiotic-loaded-microspheres-as-antimicrobial-delivery-systems-for-medical-applications
#20
J Szczeblinska, K Fijalkowski, J Kohn, M El Fray
In this paper, we present the preparation and antibiotic loading of polymeric microspheres, composed of copolymers derived from fatty acid/amino acid components, as new polymeric platforms for antibiotic delivery systems. New polymeric materials were used to prepare microspheres with and without immobilized model antibiotics (streptomycin, chloramphenicol and amphotericin B) by a W/O/W double-emulsion/solvent evaporation method, in which chloroform and poly(vinyl alcohol) are used as the solvent and emulsifier, respectively...
August 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
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