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https://www.readbyqxmd.com/read/27922184/europium-iii-and-uranium-vi-complexation-by-natural-organic-matter-nom-effect-of-source
#1
Ralf Kautenburger, Jonas M Sander, Christina Hein
For the safe long-term storage of high level radioactive waste (HLW), detailed information about geo-chemical behavior of radioactive and toxic metal ions under environmental conditions is important. Natural organic matter (NOM) can play a crucial role in the immobilization or mobilization of these metal ions due to its complexation and colloid formation tendency. In this study, the complexation of europium (as chemical homologue of trivalent actinides like americium) and uranium (as main component of HLW) by ten humic acids (HA) from different sources and Suwannee NOM river extract has been analyzed...
December 6, 2016: Electrophoresis
https://www.readbyqxmd.com/read/27922086/photoelectrochemical-detection-of-alpha-fetoprotein-based-on-zno-inverse-opals-structure-electrodes-modified-by-ag2s-nanoparticles
#2
Yandong Jiang, Dali Liu, Yudan Yang, Ru Xu, Tianxiang Zhang, Kuang Sheng, Hongwei Song
In this work, a new photoelectrochemical biosensor based on Ag2S nanoparticles (NPs) modified macroporous ZnO inverse opals structure (IOs) was developed for sensitive and rapid detection of alpha fetal protein (AFP). Small size and uniformly dispersed Ag2S NPs were prepared using the Successive Ionic Layer Adsorption And Reaction (SILAR) method, which were adsorbed on ZnO IOs surface and frame work as matrix for immobilization of AFP. The composite structure of ZnO/Ag2S expanded the scope of light absorption to long wavelength, which can make full use of the light energy...
December 6, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27920890/mesoporous-silica-nanoparticles-equipped-with-surface-nanovalves-for-ph-controlled-liberation-of-doxorubicin
#3
N V Roik, L A Belyakova
Silica carriers equipped with molecular and supramolecular pH-sensitive nanovalves were designed by combination of sol-gel synthesis and selective postsynthetic modification. Mesoporous structure of synthesized materials was characterized by low-temperature nitrogen adsorption-desorption, small-angle X-ray diffraction and transmission electron microscopy. Chemical immobilization of N-[N'-(N'-phenyl)-2-aminophenyl]aminoalkyl groups was confirmed by IR spectral and chemical analyses of surface layer. Loading and release behaviour of synthesized drug carriers was studied in phosphate buffer solutions with pH 5...
December 6, 2016: Interface Focus
https://www.readbyqxmd.com/read/27919810/immobilization-of-indigenous-holocellulase-on-iron-oxide-fe2o3-nanoparticles-enhanced-hydrolysis-of-alkali-pretreated-paddy-straw
#4
Ajay Kumar, Surender Singh, Rameshwar Tiwari, Renu Goel, Lata Nain
The holocellulase from Aspergillus niger SH3 was characterized and found to contain 125 proteins including cellulases (26), hemicellulases (21), chitinases (10), esterases (6), amylases (4) and hypothetical protein (32). The crude enzyme was immobilized on five different nanoparticles (NPs) via physical adsorption and covalent coupling methods. The enzyme-nanoparticle complexes (ENC) were screened for protein binding, enzymatic activities and immobilization efficiency. Magnetic enzyme-nanoparticle complexes (MENC) formulation showed higher immobilization efficiency (60- 80%) for most of the enzymes...
December 2, 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27919703/prediction-and-mechanism-elucidation-of-analyte-retention-on-phospholipid-stationary-phases-iam-hplc-by-in-silico-calculated-physico-chemical-descriptors
#5
Giacomo Russo, Lucia Grumetto, Francesco Barbato, Giulio Vistoli, Alessandro Pedretti
The present study proposes a method for an in silico calculation of phospholipophilicity. Phospholipophilicity is intended as the measure of analyte affinity for phospholipids; it is currently assessed by HPLC measures of analyte retention on phosphatidylcholine-like stationary phases (IAM - Immobilized Artificial Membrane) resulting in log kW(IAM) values. Due to the amphipathic and electrically charged nature of phospholipids, retention on these stationary phases results from complex mechanisms, being affected not only by lipophilicity (as measured by n-octanol/aqueous phase partition coefficients, log P) but also by the occurrence of polar and/or electrostatic intermolecular interaction forces...
December 2, 2016: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/27919320/calcium-mediated-actin-reset-caar-mediates-acute-cell-adaptations
#6
Pauline Wales, Christian E Schuberth, Roland Aufschnaiter, Johannes Fels, Ireth García-Aguilar, Annette Janning, Christopher P Dlugos, Marco Schäfer-Herte, Christoph Klingner, Mike Wälte, Julian Kuhlmann, Ekaterina Menis, Laura Hockaday Kang, Kerstin C Maier, Wenya Hou, Antonella Russo, Henry N Higgs, Hermann Pavenstädt, Thomas Vogl, Johannes Roth, Britta Qualmann, Michael M Kessels, Dietmar E Martin, Bela Mulder, Roland Wedlich-Söldner
Actin has well established functions in cellular morphogenesis. However, it is not well understood how the various actin assemblies in a cell are kept in a dynamic equilibrium, in particular when cells have to respond to acute signals. Here, we characterize a rapid and transient actin reset in response to increased intracellular calcium levels. Within seconds of calcium influx, the formin INF2 stimulates filament polymerization at the endoplasmic reticulum (ER), while cortical actin is disassembled. The reaction is then reversed within a few minutes...
December 6, 2016: ELife
https://www.readbyqxmd.com/read/27919026/hindered-cytoplasmic-diffusion-of-inositol-trisphosphate-restricts-its-cellular-range-of-action
#7
George D Dickinson, Kyle L Ellefsen, Silvina Ponce Dawson, John E Pearson, Ian Parker
The range of action of intracellular messengers is determined by their rates of diffusion and degradation. Previous measurements in oocyte cytoplasmic extracts indicated that the Ca(2+)-liberating second messenger inositol trisphosphate (IP3) diffuses with a coefficient (~280 μm(2) s(-1)) similar to that in water, corresponding to a range of action of ~25 μm. Consequently, IP3 is generally considered a "global" cellular messenger. We reexamined this issue by measuring local IP3-evoked Ca(2+) puffs to monitor IP3 diffusing from spot photorelease in neuroblastoma cells...
November 8, 2016: Science Signaling
https://www.readbyqxmd.com/read/27919018/trigeminal-nervous-system-sensitization-by-infraorbital-nerve-injury-enhances-responses-in-a-migraine-model
#8
Midori Toyama, Chiho Kudo, Chikako Mukai, Mika Inoue, Aiko Oyamaguchi, Hiroshi Hanamoto, Mitsutaka Sugimura, Hitoshi Niwa
BACKGROUND: Although the peripheral and central sensitizations of trigeminal nervous system may be one of the important factors of migraine, the precise mechanism is not fully understood. In this study, we examined the influence of the sensitization of the second division of the trigeminal nerve (V2) by chronic constriction injury (CCI) of the infraorbital nerve (ION) on migraine headache, using the capsaicin-induced migraine model. METHODS: Male Sprague-Dawley rats were assigned to four groups: (a) sham surgery and topical-dural vehicle application (Sham + Vehicle) group, (b) CCI-ION and topical-dural vehicle application (CCI-ION + Vehicle) group, (c) sham surgery and topical-dural capsaicin application (Sham + Capsaicin) group, (d) CCI-ION and topical-dural capsaicin application (CCI-ION + Capsaicin) group...
November 9, 2016: Cephalalgia: An International Journal of Headache
https://www.readbyqxmd.com/read/27918922/removal-of-heavy-metals-from-acid-mine-drainage-using-chicken-eggshells-in-column-mode
#9
Ting Zhang, Zhihong Tu, Guining Lu, Xingchun Duan, Xiaoyun Yi, Chuling Guo, Zhi Dang
Chicken eggshells (ES) as alkaline sorbent were immobilized in a fixed bed to remove typical heavy metals from acid mine drainage (AMD). The obtained breakthrough curves showed that the breakthrough time increased with increasing bed height, but decreased with increasing flow rate and increasing particle size. The Thomas model and bed depth service time model could accurately predict the bed dynamic behavior. At a bed height of 10 cm, a flow rate of 10 mL/min, and with ES particle sizes of 0.18-0.425 mm, for a multi-component heavy metal solution containing Cd(2+), Pb(2+) and Cu(2+), the ES capacities were found to be 1...
December 2, 2016: Journal of Environmental Management
https://www.readbyqxmd.com/read/27918117/effects-of-joint-immobilization-on-changes-in-myofibroblasts-and-collagen-in-the-rat-knee-contracture-model
#10
Ryo Sasabe, Junya Sakamoto, Kyo Goto, Yuichiro Honda, Hideki Kataoka, Jiro Nakano, Tomoki Origuchi, Daisuke Endo, Takehiko Koji, Minoru Okita
The purpose of this study was to examine the time-dependent changes in the development of joint capsule fibrosis and in the number of myofibroblasts in the joint capsule after immobilization, using a rat knee contracture model. Both knee joints were fixed in full flexion for 1, 2, and 4 weeks (immobilization group). Untreated rats were bred for each immobilization period (control group). Histological analysis was performed to evaluate changes in the amount and density of collagen in the joint capsule. The changes in type I and III collagen mRNA were examined by in situ hybridization...
December 5, 2016: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://www.readbyqxmd.com/read/27917913/synergistically-strengthened-3d-micro-scavenger-cage-adsorbent-for-selective-removal-of-radioactive-cesium
#11
Sung-Chan Jang, Sung-Min Kang, Yuvaraj Haldorai, Krishnan Giribabu, Go-Woon Lee, Young-Chul Lee, Moon Seop Hyun, Young-Kyu Han, Changhyun Roh, Yun Suk Huh
A novel microporous three-dimensional pomegranate-like micro-scavenger cage (P-MSC) composite has been synthesized by immobilization of iron phyllosilicates clay onto a Prussian blue (PB)/alginate matrix and tested for the removal of radioactive cesium from aqueous solution. Experimental results show that the adsorption capacity increases with increasing the inactive cesium concentration from 1 ppm to 30 ppm, which may be attributed to greater number of adsorption sites and further increase in the inactive cesium concentration has no effect...
December 5, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27917636/-immune-mediated-necrotizing-myopathy-associated-with-statin-treatment
#12
Jiří Vencovský
Immune mediated necrotizing myopathy (IMNM) is an acquired inflammatory myopathy. One form of the disease is associated with serum anti-HMGCR autoantibodies and significant proportion of patients has a history of statin treatment. The disease is rare with the incidence estimated around 23 cases/100 000 statin treated individuals. In contrast to direct statin toxicity, anti-HMGCR associated IMNM is an induced autoimmune disease.Patients suffer particularly from severe muscle weakness, which may be occasionally totally immobilizing...
2016: Casopís Lékar̆ů C̆eských
https://www.readbyqxmd.com/read/27917366/penicillin-acylase-catalyzed-synthesis-of-n-bromoacetyl-7-aminocephalosporanic-acid-the-key-intermediate-for-the-production-of-cefathiamidine
#13
Xiao-Li Zhang, Min-Hua Zong, Ning Li
BACKGROUND: Enzymatic approaches have become promising alternatives to chemical methods for the production of semi-synthetic β-lactam antibiotics. In this work, enzymatic synthesis of N-bromoacetyl-7-aminocephalosporanic acid (N-bromoacetyl-7-ACA), the key intermediate for the production of cefathiamidine, was reported for the first time. RESULTS: Of the immobilized penicillin acylases (PAs) tested, PGA-750 was the best biocatalyst. Optimization of the biocatalytic process was conducted...
2016: Bioresources and Bioprocessing
https://www.readbyqxmd.com/read/27916931/manipulation-and-immobilization-of-a-single-fluorescence-nanosensor-for-selective-injection-into-cells
#14
Hairulazwan Hashim, Hisataka Maruyama, Taisuke Masuda, Fumihito Arai
Manipulation and injection of single nanosensors with high cell viability is an emerging field in cell analysis. We propose a new method using fluorescence nanosensors with a glass nanoprobe and optical control of the zeta potential. The nanosensor is fabricated by encapsulating a fluorescence polystyrene nanobead into a lipid layer with 1,3,3-trimethylindolino-6'-nitrobenzopyrylospiran (SP), which is a photochromic material. The nanobead contains iron oxide nanoparticles and a temperature-sensitive fluorescent dye, Rhodamine B...
December 1, 2016: Sensors
https://www.readbyqxmd.com/read/27916899/label-free-detection-of-human-glycoprotein-cga-using-an-extended-gated-organic-transistor-based-immunosensor
#15
Tsukuru Minamiki, Tsuyoshi Minami, Yui Sasaki, Shin-Ichi Wakida, Ryoji Kurita, Osamu Niwa, Shizuo Tokito
Herein, we report on the fabrication of an extended-gated organic field-effect transistor (OFET)-based immunosensor and its application in the detection of human chromogranin A (hCgA). The fabricated OFET device possesses an extended-gate electrode immobilized with an anti-CgA antibody. The titration results of hCgA showed that the electrical changes in the OFET characteristics corresponded to the glycoprotein recognition ability of the monoclonal antibody (anti-CgA). The observed sensitivity (detection limit: 0...
November 30, 2016: Sensors
https://www.readbyqxmd.com/read/27916560/thinning-of-articular-cartilage-after-joint-unloading-or-immobilization-an-experimental-investigation-of-the-pathogenesis-in-mice
#16
Masato Nomura, Naoyoshi Sakitani, Hiroyuki Iwasawa, Yuta Kohara, Shoko Takano, Yoshio Wakimoto, Hiroshi Kuroki, Hideki Moriyama
OBJECTIVE: Moderate mechanical stress generated by normal joint loading and movement is essential for the maintenance of healthy articular cartilage. However, the effects of reduced loading caused by the absence of weight bearing or joint motion on articular cartilage and subchondral bone is still poorly understood. We aimed to characterize morphological and metabolic responses of articular cartilage and subchondral bone to decreased mechanical stress in vivo. METHODS: Mice were subjected to periods of hindlimb unloading by tail suspension or external fixation of the knee joints...
December 1, 2016: Osteoarthritis and Cartilage
https://www.readbyqxmd.com/read/27916536/a-new-lateral-flow-strip-assay-lfsa-using-a-pair-of-aptamers-for-the-detection-of-vaspin
#17
Nurul Hanun Ahmad Raston, Van-Thuan Nguyen, Man Bock Gu
A new lateral flow strip assay (LFSA) using a pair of aptamers has been designed and successfully developed using a pair of aptamers-functionalized with gold nanoparticles (AuNPs). This new LFSA biosensor system utilizes a cognate aptamer duo binding to vaspin, a target protein, at the two different binding sites, and exhibited a sensitive and highly selective response to vaspin. A sandwich-type format in LFSA was developed based on biotin-labeled primary V1 aptamer immobilized on streptavidin coated membrane as a capturing probe and secondary V49 aptamer conjugated with AuNPs as a signaling probe...
November 28, 2016: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27916388/application-of-dual-protease-column-for-hdx-ms-analysis-of-monoclonal-antibodies
#18
Sasidhar N Nirudodhi, Justin B Sperry, Jason C Rouse, James A Carroll
A co-immobilized, dual protease column was developed and implemented to more efficiently digest IgG molecules for hydrogen/deuterium exchange mass spectrometry (HDX-MS). The low-pH proteolytic enzymes pepsin and type XIII protease from Aspergillus were packed into a single column to most effectively combine the complementary specificities. The method was optimized using an IgG2 monoclonal antibody as a substrate because they are known to be more difficult to efficiently digest. The general applicability of the method was then demonstrated using IgG1 and IgG4 mAbs...
December 1, 2016: Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/27916114/competitive-inhibition-assay-for-the-detection-of-progesterone-in-dairy-milk-using-a-fiber-optic-spr-biosensor
#19
D Daems, J Lu, F Delport, N Mariën, L Orbie, B Aernouts, I Adriaens, T Huybrechts, W Saeys, D Spasic, J Lammertyn
Analytical methods that are often used for the quantification of progesterone in bovine milk include immunoassays and chromatographic techniques. Depending on the selected method, the main disadvantages are the cost, time-to-result, labor intensity and usability as an automated at-line device. This paper reports for the first time on a robust and practical method to quantify small molecules, such as progesterone, in complex biological samples using an automated fiber optic surface plasmon resonance (FO-SPR) biosensor...
January 15, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/27916021/use-of-point-of-care-ultrasound-in-long-bone-fractures-a-systematic-review-and-meta-analysis
#20
Lucas B Chartier, Laura Bosco, Lauren Lapointe-Shaw, Jordan Chenkin
OBJECTIVES: Long bone fractures (LBFs) are among the most frequent traumatic injuries seen in emergency departments. Reduction and immobilization is the most common form of treatment for displaced fractures. Point-of-care ultrasound (PoCUS) is a promising technique for diagnosing LBFs and assessing the success of reduction attempts. This article offers a comprehensive review of the use of PoCUS for the diagnosis and reduction of LBFs. Data source MEDLINE and EMBASE databases were searched through July 19, 2015...
December 5, 2016: CJEM
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