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https://www.readbyqxmd.com/read/27914282/fam3c-modulates-osteogenic-differentiation-by-down-regulating-runx2
#1
Ameya Bendre, Kalman G Büki, Jorma A Määttä
Fam3c, a cytokine-like protein, is a member of the Fam3 family (family with sequence similarity 3) and has been implicated to play a crucial role in Epithelial-to- mesenchymal transition (EMT) and subsequent metastasis during cancer progression. A few independent genome-wide association studies on different population cohorts predicted the gene locus of Fam3c to be associated with bone mineral density and fractures. In this study, we examined the role of Fam3c during osteoblast differentiation. Fam3c was found to be expressed during osteogenic differentiation of both primary bone marrow stromal cells and MC3T3-E1 pre-osteoblasts...
November 30, 2016: Differentiation; Research in Biological Diversity
https://www.readbyqxmd.com/read/27911291/a-metal-free-method-for-producing-mri-contrast-at-amyloid-%C3%AE
#2
Silvia Hilt, Tang Tang, Jeffrey H Walton, Madhu Budamagunta, Izumi Maezawa, Tamás Kálai, Kálmán Hideg, Vikrant Singh, Heike Wulff, Qizhi Gong, Lee-Way Jin, Angelique Louie, John C Voss
Alzheimer's disease (AD) is characterized by depositions of the amyloid-β (Aβ) peptide in the brain. The disease process develops over decades, with substantial neurological loss occurring before a clinical diagnosis of dementia can be rendered. It is therefore imperative to develop methods that permit early detection and monitoring of disease progression. In addition, the multifactorial pathogenesis of AD has identified several potential avenues for AD intervention. Thus, evaluation of therapeutic candidates over lengthy trial periods also demands a practical, noninvasive method for measuring Aβ in the brain...
November 26, 2016: Journal of Alzheimer's Disease: JAD
https://www.readbyqxmd.com/read/27906830/heterotopic-abdominal-rat-heart-transplantation-as-a-model-to-investigate-volume-dependency-of-myocardial-remodeling
#3
Kálmán Benke, Alex Ali Sayour, Csaba Mátyás, Bence Ágg, Balázs Tamás Németh, Attila Oláh, Mihály Ruppert, István Hartyánszky, Zoltán Szabolcs, Tamás Radovits, Béla Merkely, Gábor Szabó
Heterotopic abdominal rat heart transplantation has been extensively used to investigate ischemic/reperfusion injury, immunological consequences during heart transplantations and also to study remodeling of the myocardium due to volume unloading. We provide a unique review on the latter and present a summary of the experimental studies on rat heart transplantation to illustrate changes that occur to the myocardium due to volume unloading. We divided the literature based on whether normal or failing rat heart models were used...
December 1, 2016: Transplantation
https://www.readbyqxmd.com/read/27900237/design-of-a-tdoa-location-engine-and-development-of-a-location-system-based-on-chirp-spread-spectrum
#4
Rui-Rong Wang, Xiao-Qing Yu, Shu-Wang Zheng, Yang Ye
Location based services (LBS) provided by wireless sensor networks have garnered a great deal of attention from researchers and developers in recent years. Chirp spread spectrum (CSS) signaling formatting with time difference of arrival (TDOA) ranging technology is an effective LBS technique in regards to positioning accuracy, cost, and power consumption. The design and implementation of the location engine and location management based on TDOA location algorithms were the focus of this study; as the core of the system, the location engine was designed as a series of location algorithms and smoothing algorithms...
2016: SpringerPlus
https://www.readbyqxmd.com/read/27899499/differential-patterns-of-impaired-cardiorespiratory-fitness-and-cardiac-autonomic-dysfunction-in-recently-diagnosed-type-1-and-type-2-diabetes
#5
Martin Röhling, Alexander Strom, Gidon Bönhof, Sonja Püttgen, Kálmán Bódis, Karsten Müssig, Julia Szendrödi, Daniel Markgraf, Stefan Lehr, Michael Roden, Dan Ziegler
OBJECTIVE: Both impaired cardiorespiratory fitness (CRF) and heart rate variability (HRV) are predictors of mortality, but their relative roles in recent-onset diabetes are unknown. We determined to which extent CRF and HRV are reduced and interrelated in recent-onset diabetes. RESEARCH DESIGN AND METHODS: Participants from the German Diabetes Study with type 1 (n = 163) or type 2 (n = 188) diabetes with known diabetes duration <1 year and two age-matched glucose-tolerant control groups (n = 40 each) underwent spiroergometry and HRV assessment during a hyperinsulinemic-euglycemic clamp...
November 29, 2016: Diabetes Care
https://www.readbyqxmd.com/read/27898005/validation-and-parameter-sensitivity-tests-for-reconstructing-swell-field-based-on-an-ensemble-kalman-filter
#6
Xuan Wang, Pierre Tandeo, Ronan Fablet, Romain Husson, Lei Guan, Ge Chen
The swell propagation model built on geometric optics is known to work well when simulating radiated swells from a far located storm. Based on this simple approximation, satellites have acquired plenty of large samples on basin-traversing swells induced by fierce storms situated in mid-latitudes. How to routinely reconstruct swell fields with these irregularly sampled observations from space via known swell propagation principle requires more examination. In this study, we apply 3-h interval pseudo SAR observations in the ensemble Kalman filter (EnKF) to reconstruct a swell field in ocean basin, and compare it with buoy swell partitions and polynomial regression results...
November 25, 2016: Sensors
https://www.readbyqxmd.com/read/27895993/non-stationary-reconstruction-for-dynamic-fluorescence-molecular-tomography-with-extended-kalman-filter
#7
Xin Liu, Hongkai Wang, Zhuangzhi Yan
Dynamic fluorescence molecular tomography (FMT) plays an important role in drug delivery research. However, the majority of current reconstruction methods focus on solving the stationary FMT problems. If the stationary reconstruction methods are applied to the time-varying fluorescence measurements, the reconstructed results may suffer from a high level of artifacts. In addition, based on the stationary methods, only one tomographic image can be obtained after scanning one circle projection data. As a result, the movement of fluorophore in imaged object may not be detected due to the relative long data acquisition time (typically >1 min)...
November 1, 2016: Biomedical Optics Express
https://www.readbyqxmd.com/read/27889134/neural-adaptive-observer-based-sensor-and-actuator-fault-detection-in-nonlinear-systems-application-in-uav
#8
Alireza Abbaspour, Payam Aboutalebi, Kang K Yen, Arman Sargolzaei
A new online detection strategy is developed to detect faults in sensors and actuators of unmanned aerial vehicle (UAV) systems. In this design, the weighting parameters of the Neural Network (NN) are updated by using the Extended Kalman Filter (EKF). Online adaptation of these weighting parameters helps to detect abrupt, intermittent, and incipient faults accurately. We apply the proposed fault detection system to a nonlinear dynamic model of the WVU YF-22 unmanned aircraft for its evaluation. The simulation results show that the new method has better performance in comparison with conventional recurrent neural network-based fault detection strategies...
November 23, 2016: ISA Transactions
https://www.readbyqxmd.com/read/27884734/nitric-oxide-coupled-signaling-in-odor-elicited-molecular-events-in-the-olfactory-center-of-the-terrestrial-snail-helix-pomatia
#9
Zoltán Serfőző, Kálmán Nacsa, Zoltán Veréb, Izabella Battonyai, Csaba Hegedűs, Csilla Balogh, Károly Elekes
Olfaction, a chemosensory modality, plays a pivotal role in the orientation and behavior of invertebrates. The central olfactory processing unit in terrestrial stylomatophoran snails is the procerebrum, which contains NO synthesizing interneurons, whose oscillatory currents are believed to be the base of odor evoked memory formation. Nevertheless, in this model the up- and downstream events of molecular cascades that trigger and follow NO release, respectively, have not been studied. Immunocytochemistry and flow cytometry studies performed on procerebral neural perikarya isolated from the snail Helix pomatia revealed cell populations with discrete DAF-2 fluorescence, indicating the release of different amounts of NO...
November 21, 2016: Cellular Signalling
https://www.readbyqxmd.com/read/27884249/long-term-arrhythmic-and-nonarrhythmic-outcomes-of-lamin-a-c-mutation-carriers
#10
Saurabh Kumar, Samuel H Baldinger, Estelle Gandjbakhch, Philippe Maury, Jean-Marc Sellal, Alexander F A Androulakis, Xavier Waintraub, Philippe Charron, Anne Rollin, Pascale Richard, William G Stevenson, Ciorsti J Macintyre, Carolyn Y Ho, Tina Thompson, Jitendra K Vohra, Jonathan M Kalman, Katja Zeppenfeld, Frederic Sacher, Usha B Tedrow, Neal K Lakdawala
BACKGROUND: Mutations in LMNA are variably expressed and may cause cardiomyopathy, atrioventricular block (AVB), or atrial arrhythmias (AAs) and ventricular arrhythmias (VA). Detailed natural history studies of LMNA-associated arrhythmic and nonarrhythmic outcomes are limited, and the prognostic significance of the index cardiac phenotype remains uncertain. OBJECTIVES: This study sought to describe the arrhythmic and nonarrhythmic outcomes of LMNA mutation carriers and to assess the prognostic significance of the index cardiac phenotype...
November 29, 2016: Journal of the American College of Cardiology
https://www.readbyqxmd.com/read/27873279/analysis-of-viral-epigenotypes-using-chromatin-immunoprecipitation
#11
Ferenc Bánáti, Kálmán Szenthe
Chromatin Immunoprecipitation (ChIP) is a method used to detect DNA-protein interactions in vivo. ChIP has been widely applied to assess the abundance of various epigenetic regulators, including modified histones, in various regions of cellular and viral chromatin. During the procedure, DNA binding proteins are covalently cross-linked to DNA, and the isolated chromatin is broken into pieces of 300-500 bps in length on average. Thereafter, using specific antibody directed against the protein of interest the covalently cross-linked DNA is pulled down together with Protein A or G carrying beads that bind the Fc fragment of the antibody...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27873276/characterization-of-ebv-promoters-and-coding-regions-by-sequencing-pcr-amplified-dna-fragments
#12
Kalman Szenthe, Ferenc Bánáti
DNA sequencing approaches originally developed in two directions, the chemical degradation method and the chain-termination method. The latter one became more widespread and a huge amount of sequencing data including whole genome sequences accumulated, based on the use of capillary sequencer systems and the application of a modified chain-termination method which proved to be relatively easy, fast, and reliable. In addition, relatively long, up to 1000 bp sequences could be obtained with a single read with high per-base accuracy...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27873275/terminal-repeat-analysis-of-ebv-genomes
#13
Ferenc Bánáti, Anita Koroknai, Kálmán Szenthe
Epstein-Barr virus (EBV) was the first human virus associated directly with human malignancies. During EBV infection of various host cells the double-stranded linear EBV DNA carried by the virions undergoes circularization. Since there are variable numbers of terminal repetitions (TRs) at the ends of the linear EBV genome, the resulting circular episomes enclose a variable number of TRs. Thus, in cells carrying viral episomes, the sizes of the terminal restriction enzyme fragments of EBV is affected by the number of TRs (Raab-Traub and Flynn Cell 47:883-889, 1986)...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27873271/analysis-of-viral-promoter-usage-in-ebv-infected-cell-lines-a-comparison-of-qpcr-following-conventional-rna-isolation-and-nuclear-run-on-assay
#14
Kalman Szenthe, Ferenc Banati
To interpret the results of an epigenetic analysis in gene expression studies, it is essential to characterize the activity of the relevant promoters. According to the literature, real-time PCR assay is the most widely used method for the determination of latent EBV promoter usage. Here we describe two alternative approaches to measure the activity of viral promoters in cell lines carrying latent EBV episomes. The widespread typical approach relies on total cellular RNA isolation, whereas the nuclear run-on assay described here is based on the initial isolation of nuclei, followed by in vitro transcription in the presence of biotinylated-UTP, and purification of RNA transcripts using avidin-coated magnetic beads...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27869845/angular-velocity-estimation-based-on-star-vector-with-improved-current-statistical-model-kalman-filter
#15
Hao Zhang, Yanxiong Niu, Jiazhen Lu, He Zhang
Angular velocity information is a requisite for a spacecraft guidance, navigation, and control system. In this paper, an approach for angular velocity estimation based merely on star vector measurement with an improved current statistical model Kalman filter is proposed. High-precision angular velocity estimation can be achieved under dynamic conditions. The amount of calculation is also reduced compared to a Kalman filter. Different trajectories are simulated to test this approach, and experiments with real starry sky observation are implemented for further confirmation...
November 20, 2016: Applied Optics
https://www.readbyqxmd.com/read/27869715/randomized-prospective-double-blind-studies-to-evaluate-the-cognitive-effects-of-inositol-stabilized-arginine-silicate-in-healthy-physically-active-adults
#16
Douglas Kalman, Philip D Harvey, Sara Perez Ojalvo, James Komorowski
Inositol-stabilized arginine silicate (ASI; Nitrosigine(®)) has been validated to increase levels of arginine, silicon and nitric oxide production. To evaluate potential enhancement of mental focus and clarity, ASI (1500 mg/day) was tested in two double-blind placebo-controlled crossover (DBPC-X) studies using the Trail Making Test (TMT, Parts A and B). In the two studies, healthy males took ASI for 14 and 3 days, respectively. In the first study, after 14 days of dosing, TMT B time decreased significantly from baseline (28% improvement, p = 0...
November 18, 2016: Nutrients
https://www.readbyqxmd.com/read/27867353/simultaneous-bayesian-estimation-of-excitatory-and-inhibitory-synaptic-conductances-by-exploiting-multiple-recorded-trials
#17
Milad Lankarany, Jaime E Heiss, Ilan Lampl, Taro Toyoizumi
Advanced statistical methods have enabled trial-by-trial inference of the underlying excitatory and inhibitory synaptic conductances (SCs) of membrane-potential recordings. Simultaneous inference of both excitatory and inhibitory SCs sheds light on the neural circuits underlying the neural activity and advances our understanding of neural information processing. Conventional Bayesian methods can infer excitatory and inhibitory SCs based on a single trial of observed membrane potential. However, if multiple recorded trials are available, this typically leads to suboptimal estimation because they neglect common statistics (of synaptic inputs (SIs)) across trials...
2016: Frontiers in Computational Neuroscience
https://www.readbyqxmd.com/read/27855940/rheological-and-stability-aspects-of-dry-and-hydrothermally-heat-treated-aleurone-rich-wheat-milling-fraction
#18
Blanka Bucsella, Ágnes Takács, Walter von Reding, Urs Schwendener, Franka Kálmán, Sándor Tömösközi
Novel aleurone-rich wheat milling fraction developed and produced on industry scale is investigated. The special composition of the novel flour with high protein, dietary fiber and fat content results in a unique combination of the mixing and viscosity properties. Due to the high lipid concentration, the fraction is exposed to fast rancidity. Dry heat (100°C for 12min) and hydrothermal treatment processes (96°C for 6min with 0-20 L/h steam) were applied on the aleurone-rich flour to modify the technological properties...
April 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/27854288/a-new-multi-sensor-fusion-scheme-to-improve-the-accuracy-of-knee-flexion-kinematics-for-functional-rehabilitation-movements
#19
Halim Tannous, Dan Istrate, Aziz Benlarbi-Delai, Julien Sarrazin, Didier Gamet, Marie Christine Ho Ba Tho, Tien Tuan Dao
Exergames have been proposed as a potential tool to improve the current practice of musculoskeletal rehabilitation. Inertial or optical motion capture sensors are commonly used to track the subject's movements. However, the use of these motion capture tools suffers from the lack of accuracy in estimating joint angles, which could lead to wrong data interpretation. In this study, we proposed a real time quaternion-based fusion scheme, based on the extended Kalman filter, between inertial and visual motion capture sensors, to improve the estimation accuracy of joint angles...
November 15, 2016: Sensors
https://www.readbyqxmd.com/read/27849540/multi-layer-quantization-control-for-quality-constrained-h-265-hevc
#20
Xiang Pan, Zhenzhong Chen
Achieving a stable video quality is an important task in video compression. In this paper, we present a multi-layer quantization control method to compress video to a certain target video quality based on the new hierarchical partition structure of H.265/HEVC. We first model the rate-quality characteristics of different video sequences using -support vector regression with a Gaussian radial basis function as the kernel function. Then, we propose a Kalman filter-based multi-layer quantization control method for the quality-constrained video coding...
November 10, 2016: IEEE Transactions on Image Processing: a Publication of the IEEE Signal Processing Society
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