keyword
MENU ▼
Read by QxMD icon Read
search

Piwi

keyword
https://www.readbyqxmd.com/read/29791965/searching-for-non-transposable-targets-of-planarian-nuclear-piwi-in-pluripotent-stem-cells-and-differentiated-cells
#1
Makoto Kashima, Kiyokazu Agata, Norito Shibata
Nuclear PIWIs together with their guide RNAs (piRNAs) epigenetically silence various genes including transposons in many organisms. In planarians, the nuclear piwi family gene, DjpiwiB is specifically transcribed in adult pluripotent stem cells (adult PSC, neoblast), but not in differentiated cells. However, the protein accumulates in the nuclei of both neoblasts and their descendant differentiated cells. Interestingly, PIWI(DjPiwiB)-piRNA complexes are indispensable for the repression of transposable genes at the onset of differentiation from neoblasts...
May 23, 2018: Development, Growth & Differentiation
https://www.readbyqxmd.com/read/29778390/next-generation-sequencing-reveals-differentially-expressed-small-noncoding-rnas-in-uterine-leiomyoma
#2
Tsai-Der Chuang, Yeming Xie, Wei Yan, Omid Khorram
OBJECTIVE: To determine the expression profile of small noncoding RNAs (sncRNAs) in leiomyoma, which has not been investigated to date. DESIGN: Laboratory-based investigation. SETTING: Academic center. PATIENT(S): Women undergoing hysterectomy for benign indications. INTERVENTION(S): Next-generation sequencing and screening of an sncRNA database with confirmatory analysis by quantitative reverse-transcription polymerase chain reaction (qRT-PCR)...
May 2018: Fertility and Sterility
https://www.readbyqxmd.com/read/29769402/gtsf-1-is-required-for-formation-of-a-functional-rna-dependent-rna-polymerase-complex-in-caenorhabditis-elegans
#3
Miguel Vasconcelos Almeida, Sabrina Dietz, Stefan Redl, Emil Karaulanov, Andrea Hildebrandt, Christian Renz, Helle D Ulrich, Julian König, Falk Butter, René F Ketting
Argonaute proteins and their associated small RNAs (sRNAs) are evolutionarily conserved regulators of gene expression. Gametocyte-specific factor 1 (Gtsf1) proteins, characterized by two tandem CHHC zinc fingers and an unstructured C-terminal tail, are conserved in animals and have been shown to interact with Piwi clade Argonautes, thereby assisting their activity. We identified the Caenorhabditis elegans Gtsf1 homolog, named it gtsf-1 and characterized it in the context of the sRNA pathways of C. elegans We report that GTSF-1 is not required for Piwi-mediated gene silencing...
May 16, 2018: EMBO Journal
https://www.readbyqxmd.com/read/29760316/unique-sex-determination-system-in-the-silkworm-bombyx-mori-current-status-and-beyond
#4
Susumu Katsuma, Takashi Kiuchi, Munetaka Kawamoto, Toshiaki Fujimoto, Ken Sahara
The silkworm Bombyx mori has been used for silk production for over 5,000 years. In addition to its contribution to sericulture, B. mori has played an important role in the field of genetics. Classical genetic studies revealed that a gene(s) with a strong feminizing activity is located on the W chromosome, but this W-linked feminizing gene, called Feminizer (Fem), had not been cloned despite more than 80 years of study. In 2014, we discovered that Fem is a precursor of a single W chromosome-derived PIWI-interacting RNA (piRNA)...
2018: Proceedings of the Japan Academy. Series B, Physical and Biological Sciences
https://www.readbyqxmd.com/read/29755289/decoding-the-emerging-patterns-exhibited-in-non-coding-rnas-characteristic-of-lung-cancer-with-regard-to-their-clinical-significance
#5
Laura Sonea, Mihail Buse, Diana Gulei, Anca Onaciu, Ioan Simon, Cornelia Braicu, Ioana Berindan-Neagoe
Lung cancer continues to be the leading topic concerning global mortality rate caused by can-cer; it needs to be further investigated to reduce these dramatic unfavorable statistic data. Non-coding RNAs (ncRNAs) have been shown to be important cellular regulatory factors and the alteration of their expression levels has become correlated to extensive number of pathologies. Specifically, their expres-sion profiles are correlated with development and progression of lung cancer, generating great interest for further investigation...
May 2018: Current Genomics
https://www.readbyqxmd.com/read/29752750/comparative-expression-profile-of-micrornas-and-pirnas-in-three-ruminant-species-testes-using-next-generation-sequencing
#6
H Wang, J Zhong, Z Chai, J Zhu, J Xin
microRNA (miRNA) and piwi-interacting RNA (piRNA) are two classes small non-coding regulatory RNAs that play crucial roles in multiple biological processes such as spermatogenesis. However, there are no published studies on conjoint analysis of miRNA and piRNA profiles among cattle, yak and their interspecies (the dzo) using sequencing technology. Next-generation sequencing technology was used to profile miRNAs and piRNAs among those three ruminants to elucidate their functions. A total of 119, 14 and six differentially expressed miRNAs were obtained in cattle vs...
May 11, 2018: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/29743438/improved-boronate-affinity-electrophoresis-by-optimization-of-the-running-buffer-for-a-single-step-separation-of-pirna-from-mouse-testis-total-rna
#7
Yusuke Sato, Daijiro Iwasawa, Kuo Ping Hui, Rena Nakagomi, Seiichi Nishizawa
Here we examined optimization of the running buffer in boronate affinity electrophoresis for improved separation of PIWI-interacting RNA (piRNA) with 2'-O-methylated ribose in 3'-terminal nucleotide. The use of Good's buffer, such as HEPES, significantly increased the separation efficiency for piRNA over normal RNA with free 3'-terminal ribose, and retained an ability to resolve the difference by at least 4-nucleotide lengths in the target piRNAs. We also demonstrated a single-step separation of piRNA from mouse testis total RNA...
2018: Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry
https://www.readbyqxmd.com/read/29735660/longevity-and-transposon-defense-the-case-of-termite-reproductives
#8
Daniel Elsner, Karen Meusemann, Judith Korb
Social insects are promising new models in aging research. Within single colonies, longevity differences of several magnitudes exist that can be found elsewhere only between different species. Reproducing queens (and, in termites, also kings) can live for several decades, whereas sterile workers often have a lifespan of a few weeks only. We studied aging in the wild in a highly social insect, the termite Macrotermes bellicosus , which has one of the most pronounced longevity differences between reproductives and workers...
May 7, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29735528/a-critical-role-for-nucleoporin-358-nup358-in-transposon-silencing-and-pirna-biogenesis-in-drosophila
#9
Rasesh Y Parikh, Haifan Lin, Vamsi K Gangaraju
Piwi-interacting RNAs (piRNAs) are a class of small non-coding RNAs that bind Piwi proteins to silence transposons and to regulate gene expression. In Drosophila germ cells, the Aubergine (Aub)-Argonaute 3 (Ago3)-dependent ping-pong cycle generates most germline piRNAs. Loading of anti-sense piRNAs amplified by this cycle enables Piwi to enter the nucleus and silence transposons. Nuclear localization is crucial for Piwi function in transposon silencing, but how this process is regulated remains unknown. It is also not known whether any of the components of the nuclear pore complex (NPC) directly function in the piRNA pathway...
May 7, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29730207/sports1-0-a-tool-for-annotating-and-profiling-non-coding-rnas-optimized-for-rrna-and-trna-derived-small-rnas
#10
Junchao Shi, Eun-A Ko, Kenton M Sanders, Qi Chen, Tong Zhou
High-throughput RNA-seq has revolutionized the process of small RNA (sRNA) discovery, leading to a rapid expansion of sRNA categories. In addition to the previously well-characterized sRNAs such as microRNAs (miRNAs), piwi-interacting RNAs (piRNAs), and small nucleolar RNA (snoRNAs), recent emerging studies have spotlighted on tRNA-derived sRNAs (tsRNAs) and rRNA-derived sRNAs (rsRNAs) as new categories of sRNAs that bear versatile functions. Since existing software and pipelines for sRNA annotation are mostly focused on analyzing miRNAs or piRNAs, here we developed the sRNA annotation pipelineoptimized for rRNA- and tRNA-derived sRNAs (SPORTS1...
May 3, 2018: Genomics, Proteomics & Bioinformatics
https://www.readbyqxmd.com/read/29728561/heterochromatin-protein-1a-functions-for-pirna-biogenesis-predominantly-from-pericentric-and-telomeric-regions-in-drosophila
#11
Ryan Yee Wei Teo, Amit Anand, Vishweshwaren Sridhar, Katsutomo Okamura, Toshie Kai
In metazoan germline, Piwi-interacting RNAs (piRNAs) provide defence against transposons. Piwi-piRNA complex mediates transcriptional silencing of transposons in nucleus. Heterochromatin protein 1a (HP1a) has been proposed to function downstream of Piwi-piRNA complex in Drosophila. Here we show that HP1a germline knockdown (HP1a-GLKD) leads to a reduction in the total and Piwi-bound piRNAs mapping to clusters and transposons insertions, predominantly in the regions close to telomeres and centromeres, resulting in derepression of a limited number of transposons from these regions...
May 4, 2018: Nature Communications
https://www.readbyqxmd.com/read/29703750/identification-of-substrates-of-the-small-rna-methyltransferase-hen1-in-mouse-spermatogonial-stem-cells-and-analysis-of-its-methyl-transfer-domain
#12
Ling Peng, Fengjuan Zhang, Renfu Shang, Xueyan Wang, Jiayi Chen, James J Chou, Jinbiao Ma, Ligang Wu, Ying Huang
Small noncoding RNAs (sncRNAs) regulate many genes in eukaryotic cells. Hua enhancer 1 (Hen1) is a 2'-O-methyltransferase that adds a methyl group to the 2'-OH of 3'-terminal nucleotide of sncRNAs. The types and properties of sncRNAs may vary among different species, and the domain composition, structure, and function of Hen1 proteins differ accordingly. In mammals, Hen1 specifically methylates sncRNAs called p-element-induced wimpy testis (PIWI)-interacting RNAs (piRNAs). However, other types of sncRNAs that are methylated by Hen1 have not yet been reported, and the structures and the substrates of mammalian Hen1 remain unknown...
April 27, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29701755/domain-functional-analyses-of-piwil1-and-pabpc1-indicate-their-synergistic-roles-in-protein-translation-via-3-utrs-of-meiotic-mrnas
#13
Yaoyao Wu, Kaibiao Xu, Huayu Qi
Translational regulation plays a central role during post-meiotic development of male germ cells. Previous studies suggested that PIWIL1, a PIWI-interacting RNA (piRNA) binding protein that is critical for sperm development, participates in the maintenance and translational regulation of post-meiotic mRNAs in haploid spermatids. However, how PIWIL1 regulates protein translation remains largely unclear. Using biochemical assays, we show here that PIWIL1 utilizes different domains to interact with post-meiotic mRNAs and PABPC1, a general protein translation regulator...
April 25, 2018: Biology of Reproduction
https://www.readbyqxmd.com/read/29696996/a-technical-assessment-of-the-porcine-ejaculated-spermatozoa-for-a-sperm-specific-rna-seq-analysis
#14
Marta Gòdia, Fabiana Quoos Mayer, Julieta Nafissi, Anna Castelló, Joan Enric Rodríguez-Gil, Armand Sánchez, Alex Clop
The study of the boar sperm transcriptome by RNA-seq can provide relevant information on sperm quality and fertility and might contribute to animal breeding strategies. However, the analysis of the spermatozoa RNA is challenging as these cells harbor very low amounts of highly fragmented RNA, and the ejaculates also contain other cell types with larger amounts of non-fragmented RNA. Here, we describe a strategy for a successful boar sperm purification, RNA extraction and RNA-seq library preparation. Using these approaches our objectives were: (i) to evaluate the sperm recovery rate (SRR) after boar spermatozoa purification by density centrifugation using the non-porcine-specific commercial reagent BoviPureTM ; (ii) to assess the correlation between SRR and sperm quality characteristics; (iii) to evaluate the relationship between sperm cell RNA load and sperm quality traits and (iv) to compare different library preparation kits for both total RNA-seq (SMARTer Universal Low Input RNA and TruSeq RNA Library Prep kit) and small RNA-seq (NEBNext Small RNA and TailorMix miRNA Sample Prep v2) for high-throughput sequencing...
April 26, 2018: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/29694837/planarian-flatworms-as-a-new-model-system-for-understanding-epigenetic-regulation-of-stem-cell-pluripotency-and-differentiation
#15
REVIEW
Anish Dattani, Divya Sridhar, Aziz Aboobaker
Planarian flatworms possess pluripotent stem cells (neoblasts) that are able to differentiate into all cell types that constitute the adult body plan. Consequently, planarians possess remarkable regenerative capabilities. Transcriptomic studies have revealed that gene expression is coordinated to maintain neoblast pluripotency, and ensure correct lineage specification during differentiation. But as yet they have not revealed how this regulation of expression is controlled. In this review, we propose that planarians represent a unique and effective system to study the epigenetic regulation of these processes in an in vivo context...
April 22, 2018: Seminars in Cell & Developmental Biology
https://www.readbyqxmd.com/read/29690553/pirna-profiling-of-dengue-virus-type-2-infected-asian-tiger-mosquito-and-midgut-tissues
#16
Yanhai Wang, Binbin Jin, Peiwen Liu, Jing Li, Xiaoguang Chen, Jinbao Gu
The Asian tiger mosquito, Aedes albopictus , is a competent vector for the majority of arboviruses. The mosquito innate immune response is a primary determinant for arthropod-borne virus transmission, and the midgut is the first barrier to pathogen transmission. Mosquito antiviral immunity is primarily mediated by the small interfering RNA pathway. However, the roles that the P-element induced wimpy testis (PIWI)-interacting RNA (piRNA) pathway play in antiviral immunity in Ae. albopictus and its midgut still need further exploration...
April 22, 2018: Viruses
https://www.readbyqxmd.com/read/29683506/noncoding-rnas-in-ischemic-stroke-time-to-translate
#17
REVIEW
Harpreet Kaur, Deepaneeta Sarmah, Jackson Saraf, Kanchan Vats, Kiran Kalia, Anupom Borah, Dileep R Yavagal, Kunjan R Dave, Zhumur Ghosh, Pallab Bhattacharya
Stroke is devastating and a major cause of morbidity and mortality around globe. Current interventions for ischemic stroke include thrombolytics, clot retrieval devices and/or intravenous tissue plasminogen activators (tPA), the latter two becoming the first line of treatment. Owing to the limitations of tPA to elicit therapeutic benefits in a narrow time window, new pharmacological interventions are needed. Exploring noncoding RNAs (ncRNAs) may be a promising option for stroke treatment. ncRNAs are endogenous molecules that play key roles in the pathophysiology of many functions and diseases, including during ischemic stroke...
April 23, 2018: Annals of the New York Academy of Sciences
https://www.readbyqxmd.com/read/29678924/sirnas-and-pirnas-collaborate-for-transposon-control-in-the-two-spotted-spider-mite
#18
Mosharrof Mondal, Kody Mansfield, Alex Flynt
RNAi has revolutionized genetic research, and is being commercialized as an insect pest control technology. Mechanisms exploited for this purpose are antiviral and therefore rapidly evolving. Ideally, RNAi will also be used for non-insect pests, however, differences in RNAi biology makes this uncertain. Tetranychus urticae (two-spotted spider mite) is a destructive non-insect pest, which has a proclivity to develop pesticide resistance. Here we provide a comprehensive study of the endogenous RNAi pathways of spider mites to inform design of exogenous RNAi triggers...
April 20, 2018: RNA
https://www.readbyqxmd.com/read/29677157/molecular-characterization-and-the-function-of-argonaute3-in-rnai-pathway-of-plutella-xylostella
#19
Muhammad Salman Hameed, Zhengbing Wang, Liette Vasseur, Guang Yang
Argonaute (Ago) protein family plays a key role in the RNA interference (RNAi) process in different insects including Lepidopteran. However, the role of Ago proteins in the RNAi pathway of Plutella xylostella is still unknown. We cloned an Argonaute3 gene in P. xylostella ( PxAgo3 ) with the complete coding sequence of 2832 bp. The encoded protein had 935 amino acids with an expected molecular weight of 108.9 kDa and an isoelectric point of 9.29. It contained a PAZ (PIWI/Argonaute/Zwile) domain and PIWI (P-element-induced whimpy testes) domain...
April 20, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29667432/identification-and-characterisation-of-micrornas-and-piwi-interacting-rnas-in-cockerels-spermatozoa-by-solexa-sequencing
#20
Shengru Wu, Jiacheng Guo, Liqin Zhu, Jiantao Yang, Si Chen, Xiaojun Yang
1. There has been substantial research focused on the roles of microRNAs (miRNA) and PIWI-interacting RNAs (piRNA) delivered from mammalian spermatozoa, however comparatively little is known about the role of spermatozoa-delivered miRNAs and piRNAs within breeding cockerels' spermatozoa. 2. A small RNA library of cockerels' spermatozoa was constructed using Illumina high-throughput sequencing technology. Unique sequences with lengths of 18-26 nucleotides were mapped to miRBase 21.0 and unique sequences with lengths of 25-37 nucleotides were mapped to a piRNA database...
April 18, 2018: British Poultry Science
keyword
keyword
31123
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"