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Olof Beck, Naama Kenan Modén, Sabina Seferaj, Gabriel Lenk, Anders Helander
Phosphatidylethanol (PEth) is a group of phospholipids formed in cell membranes following alcohol consumption. PEth measurement in whole blood samples is established as a specific alcohol biomarker with clinical and medico-legal applications. This study further evaluated the usefulness of dried blood spot (DBS) samples collected on filter paper for PEth measurement. Specimens used were surplus volumes of venous whole blood sent for routine LC-MS/MS quantification of PEth 16:0/18:1, the major PEth homolog. DBS samples were prepared by pipetting blood on Whatman 903 Protein Saver Cards and onto a volumetric DBS device (Capitainer)...
January 5, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
Tao Lin, Tangbo Li, Heng Jiang, Jun Ma, Xuhui Zhou
OBJECTION: To compare the use of uniplanar and multiaxial pedicle screws in terms of their effects on the apical vertebral derotation efficiency in Lenke V AIS patients. METHODS: A retrospective study was performed, and Lenke V AIS patients with uniplanar pedicle screws (Group I) and multiaxial pedicle screws (Group II) were collected from Jan 2013 to Dec 2015. The grade of the apical vertebral rotation was evaluated before and after surgery using the Nash-Moe and Upasani methods, respectively...
December 28, 2017: World Neurosurgery
Sandra L Bixler, Thomas M Bocan, Jay Wells, Kelly Wetzel, Sean Van Tongeren, Lian Dong, Nicole Lackemeyer, Ginger Donnelly, Lisa Cazares, Jonathan Nuss, Veronica Soloveva, Keith Koistinen, Lisa Welch, Carol Epstein, Li-Fang Liang, Dennis Giesing, Robert Lenk, Sina Bavari, Travis K Warren
Favipiravir is a broad-spectrum antiviral agent that has demonstrated efficacy against Ebola virus (EBOV) in rodents. However, there are no published reports of favipiravir efficacy for filovirus infection of nonhuman primates (NHPs). Here we evaluated the pharmacokinetic profile of favipiravir in NHPs, as well as in vivo efficacy against two filoviruses, EBOV and Marburg virus (MARV). While no survival benefit was observed in two studies employing once- or twice-daily oral dosing of favipiravir during EBOV infection of NHPs, an antiviral effect was observed in terms of extended time-to-death and reduced levels of viral RNA...
December 28, 2017: Antiviral Research
Sandra L Bixler, Thomas M Bocan, Jay Wells, Kelly Wetzel, Sean Van Tongeren, Nicole Lackemeyer, Ginger Donnelly, Lisa Cazares, Veronica Soloveva, Lisa Welch, Carol Epstein, Li-Fang Liang, Dennis Giesing, Robert Lenk, Sina Bavari, Travis K Warren
During the 2013-2016 Ebola virus (EBOV) outbreak in West Africa, our team at USAMRIID evaluated the antiviral activity of a number of compounds, including favipiravir (T-705), in vitro and in mouse and nonhuman primate (NHP) models of Ebola virus disease. In this short communication, we present our findings for favipiravir in cell culture and in mice, while an accompanying paper presents the results of NHP studies. We confirmed previous reports that favipiravir has anti-EBOV activity in mice. Additionally, we found that the active form of favipiravir is generated in mice in tissues relevant for the pathogenesis of EBOV infection...
December 28, 2017: Antiviral Research
Jeffrey L Gum, Lawrence G Lenke, Anand Mohapatra, Sam Q Sun, Michael P Kelly
STUDY DESIGN: Retrospective review. OBJECTIVES: To describe 3 cases of a posterior-only vertebral column resection (pVCR) for the treatment of spondyloptosis in the setting of prior spinal fusions. SUMMARY OF BACKGROUND DATA: Lumbosacral spondyloptosis is a rare spinal deformity with a number of surgical options, none of which demonstrate clear superiority. The use of an L5 vertebral column resection, via combined anterior and posterior approaches, to restore lumbosacral alignment has been described though is accompanied by high rates of neurological deficit...
January 2018: Spine Deformity
Dawn M Nederhoff, Kathleen M Lenk, Keith J Horvath, Toben F Nelson, Alexandra M Ecklund, Darin J Erickson, Traci L Toomey
Excessive alcohol consumption can result from illegal sales to intoxicated patrons at bars and restaurants. We surveyed bar/restaurant managers about their practices in reducing illegal sales to intoxicated patrons. We found that managers were confident that they could refuse service to intoxicated customers but were less likely to have communicated necessary information to their staff on how to refuse such sales. Managers who agreed that a business in their community would be cited for overservice were more likely to be confident that they could handle customers who had been cut off from alcohol service...
January 1, 2017: Journal of Drug Education
Maria Syngelaki, Matthias Hardner, Patrick Oberthuer, Thomas Bley, Danilo Schneider, Felix Lenk
A novel, non-destructive method for the biomass estimation of biological samples on culture dishes was developed. To achieve this, a photogrammetric system, which consists of a digital single-lens reflex camera (DSLR), an illuminated platform where the culture dishes are positioned and an Arduino board which controls the capturing process, was constructed. The camera was mounted on a holder which set the camera at different title angles and the platform rotated, to capture images from different directions. A software, based on stereo photogrammetry, was developed for the three-dimensional (3D) reconstruction of the samples...
December 11, 2017: Bioprocess and Biosystems Engineering
Belal Elnady, Mohammad M El-Sharkawi, Mohamed El-Meshtawy, Faisal F Adam, Galal Zaki Said
INTRODUCTION: The aim of this prospective case series study is to document safety and effectiveness of high density pedicle screws through posterior only approach with intraoperative wake-up test in correction of adolescent idiopathic scoliosis (AIS). METHODS: Between 2011 and 2015, all surgically treated patients for AIS were followed up for a minimum of 2 years. Clinical outcomes were evaluated using scoliosis research society-22 (SRS) questionnaire. All patients were classified according to Lenke classification...
2017: SICOT-J
Sascha Rahn, Vivien Zimmermann, Fabrice Viol, Hendrike Knaack, Kerstin Stemmer, Lena Peters, Lennart Lenk, Hendrik Ungefroren, Dieter Saur, Heiner Schäfer, Ole Helm, Susanne Sebens
Type 2 diabetes mellitus (T2DM) is associated with hyperglycemia and a risk to develop pancreatic ductal adenocarcinoma (PDAC), one of the most fatal malignancies. Cancer stem cells (CSC) are essential for initiation and maintenance of tumors, and acquisition of CSC-features is linked to epithelial-mesenchymal-transition (EMT). The present study investigated whether hyperglycemia promotes EMT and CSC-features in premalignant and malignant pancreatic ductal epithelial cells (PDEC). Under normoglycemia (5 mM d-glucose), Panc1 PDAC cells but not premalignant H6c7-kras cells exhibited a mesenchymal phenotype along with pronounced colony formation...
February 28, 2018: Cancer Letters
Kathleen M Lenk, Darin J Erickson, Jean L Forster
PURPOSE: To identify trajectories of smoking behaviors of a cohort of youth followed through young adulthood from 2000 to 2013. DESIGN: The Minnesota Adolescent Community Cohort study, a population-based cohort study. SETTING: Nationwide, originating in the Midwestern United States. PARTICIPANTS: Cohort of youth surveyed for 14 years beginning at ages 12 to 16 (N = 4241 at baseline; 59% recruitment rate). MEASURES: Main variable of interest was the number of days smoked in the past 30 days...
January 1, 2017: American Journal of Health Promotion: AJHP
Daniela Fangmann, Eva-Maria Theismann, Kathrin Türk, Dominik M Schulte, Isabelle Relling, Katharina Hartmann, Julia K Keppler, Jörg-Rainer Knipp, Ateequr Rehman, Femke-Anouska Heinsen, Andre Franke, Lennart Lenk, Sandra Freitag-Wolf, Esther Appel, Stanislav Gorb, Charles Brenner, Dirk Seegert, Georg H Waetzig, Philip Rosenstiel, Stefan Schreiber, Karin Schwarz, Matthias Laudes
OBJECTIVE: Gut microbiota represent a potential novel target for future prediabetes and type 2 diabetes therapies. In that respect, niacin has been shown to beneficially affect the host-microbiome interaction in rodent models. RESEARCH DESIGN AND METHODS: We characterized more than 500 human subjects with different metabolic phenotypes regarding their niacin (nicotinic acid [NA] and nicotinamide [NAM]) status and their gut microbiome. In addition, NA and NAM delayed-release microcapsules were engineered and examined in vitro and in vivo in two human intervention studies (bioavailability study and proof-of-concept/safety study)...
December 6, 2017: Diabetes Care
C de Bodman, F Miyanji, B Borner, P-Y Zambelli, G Racloz, R Dayer
AIMS: The aim of this study was to report a retrospective, consecutive series of patients with adolescent idiopathic scoliosis (AIS) who were treated with posterior minimally invasive surgery (MIS) with a mean follow-up of two years (sd 1.4; 0.9 to 0 3.7). Our objectives were to measure the correction of the deformity and record the peri-operative morbidity. Special attention was paid to the operating time (ORT), estimated blood loss (EBL), length of stay (LOS) and further complications...
December 2017: Bone & Joint Journal
Masashi Uehara, Jun Takahashi, Shota Ikegami, Shugo Kuraishi, Toshimasa Futatsugi, Hiroki Oba, Michihiko Koseki, Hiroyuki Kato
PURPOSE: In skip pedicle screw fixation for adolescent idiopathic scoliosis (AIS), the mid-term effects of reducing screw number on correction and clinical results are uncertain. We clarified the mid-term outcomes of this technique in patients with Lenke type 1 and 2 AIS. METHODS: Thirty-four patients who underwent skip pedicle screw fixation (mean screw density: 1.35 screws) for Lenke type 1 and 2 AIS were retrospectively reviewed. The follow-up period was at least 5 years (mean follow-up period: 6...
November 30, 2017: Journal of Orthopaedic Science: Official Journal of the Japanese Orthopaedic Association
Ozcan Konur
PURPOSE: Wei et al. evaluated the global research in spine using scientometric methods based on a sample of 13,115 papers published in 5 spine journals from 2004 to 2013. This study builds on this pioneering study and provides up-to-date and thorough information on spine based on a sample of 166,962 papers for the stakeholders. METHOD: 'Articles' and 'reviews' published in 'English' in the journals indexed by the 'Web of Science' primary databases between 1980 and 2017 were retrieved through the use of an optimal keyword set for titles of both papers and ten spine journals...
December 1, 2017: European Spine Journal
Maddalena Botti, Luisa Ragionieri, Antonio Cacchioli, Rino Panu, Ferdinando Gazza
The location, number and size of the central and peripheral neurons innervating the ischiocavernous muscle (ICM) were studied in male pigs by means of Fast Blue (FB) retrograde neuronal tracing. Moreover the immunohistochemical properties of the sympathetic ganglia were investigated combining the double immunolabelling method. After injection of FB into the left ICM, a mean number of 245.3±134.9 labelled neurons were found in the ipsilateral ventral horn of the S1-S3 segments of the spinal cord (SC), 129.7±45...
November 29, 2017: Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology
Lisa Godfrey, Antonio Iannitelli, Natalie L Garrett, Julian Moger, Ian Imbert, Tamara King, Frank Porreca, Ramesh Soundararajan, Aikaterini Lalatsa, Andreas G Schätzlein, Ijeoma F Uchegbu
The delivery of peptide drugs to the brain is challenging, principally due to the blood brain barrier and the low metabolic stability of peptides. Exclusive delivery to the brain with no peripheral exposure has hitherto not been demonstrated with brain quantification data. Here we show that polymer nanoparticles encapsulating leucine5-enkephalin hydrochloride (LENK) are able to transport LENK exclusively to the brain via the intranasal route, with no peripheral exposure and nanoparticle localisation is observed within the brain parenchyma...
November 27, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
David B Bumpass, Lawrence G Lenke, Jeffrey L Gum, Christopher I Shaffrey, Justin S Smith, Christopher P Ames, Shay Bess, Brian J Neuman, Eric Klineberg, Gregory M Mundis, Frank Schwab, Virginie Lafage, Han Jo Kim, Douglas C Burton, Khaled M Kebaish, Richard Hostin, Renaud Lafage, Michael P Kelly
OBJECTIVE Adolescent spine deformity studies have shown that male patients require longer surgery and have greater estimated blood loss (EBL) and complications compared with female patients. No studies exist to support this relationship in adult spinal deformity (ASD). The purpose of this study was to investigate associations between sex and complications, deformity correction, and health-related quality of life (HRQOL) in patients with ASD. It was hypothesized that male ASD patients would have greater EBL, longer surgery, and more complications than female ASD patients...
December 2017: Neurosurgical Focus
Raphael Pietton, Houssam Bouloussa, Claudio Vergari, Wafa Skalli, Raphael Vialle
BACKGROUND: A reproducibility study of preoperative rib cage three-dimensional (3D) measurements was conducted for patients with Adolescent Idiopathic Scoliosis (AIS). No prior reliability study has been performed for preoperative 3D reconstructions of the rib cage by using stereoradiography in patients with preoperative AIS. Our objective was to assess the reliability of rib cage 3D reconstructions using biplanar stereoradiography in patients with AIS before surgery. METHODS: This series includes 21 patients with Lenke 1 or 2 scoliosis (74±20 degrees)...
November 16, 2017: Journal of Pediatric Orthopedics
Kwan Mun Keong, Izzuddin Aziz, Chris Chan Yin Wei
PURPOSE: This study aims to derive a formula to predict post-operative height increment in Lenke 1 and Lenke 2 adolescent idiopathic scoliosis (AIS) patients using preoperative radiological parameters. METHODS: This study involved 70 consecutive Lenke 1 and 2 AIS patients who underwent scoliosis correction with alternate-level pedicle screw instrumentation. Preoperative parameters that were measured included main thoracic (MT) Cobb angle, proximal thoracic (PT) Cobb angle, lumbar Cobb angle as well as thoracic kyphosis...
January 1, 2017: Journal of Orthopaedic Surgery
I Sanpera, J Burgos-Flores, C Barrios, G Piza-Vallespir, L M Anton-Rodrigalvarez
The purpose of this study has been to evaluate the use of preoperative autologous blood donation (PABD) in the surgical treatment of adolescent idiopathic scoliosis (AIS). The surgical records of 37 AIS patients, Lenke type 1, were reviewed. Patients were classified according to whether they had entered the PABD (Program of Autologous Blood Donation) (25 patients) or had been rejected to join (12 patients non-donors). Both groups were comparable. All but one of the PADB patients (96%) as compared to only 2 non-donors patients (16...
December 2016: Acta Orthopaedica Belgica
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