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Bacterial vaginosis

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https://www.readbyqxmd.com/read/28293099/a-comparison-of-colorimetric-assessment-of-vaginal-ph-with-nugent-score-for-the-detection-of-bacterial-vaginosis
#1
Matthew K Hoffman, Mrutyunjaya B Bellad, Umesh S Charantimath, Avinash Kavi, Jyoti M Nagmoti, Mahantesh B Nagmoti, Ashalata A Mallapur, Geetanjali M Katageri, Umesh Y Ramadurg, Sheshidhar G Bannale, Amit P Revankar, M S Ganachari, Richard J Derman, Shivaprasad S Goudar
Background. A Nugent score > 7 has been defined as the gold standard for the diagnosis for bacterial vaginosis (BV), though it is resource intensive and impractical as point of care testing. We sought to determine if colorimetric assessment of vaginal pH can accurately predict the occurrence of BV. Methods. We performed a planned subanalysis of 1,216 pregnant women between 13 0/7 and 19 6/7 weeks who underwent vaginal examination as part of a randomized controlled trial. Using a standardized technique, specimens were obtained for colorimetric assessment and two separate slides for Gram staining...
2017: Infectious Diseases in Obstetrics and Gynecology
https://www.readbyqxmd.com/read/28280394/addressing-intravaginal-practices-in-women-with-hiv-and-at-risk-for-hiv-infection-a-mixed-methods-pilot-study
#2
Maria L Alcaide, Violeta J Rodriguez, Margaret A Fischl, Deborah L Jones, Stephen M Weiss
Intravaginal practices (IVPs), include intravaginal cleansing (cleansing the inside of the vagina) or intravaginal insertion of products for hygiene, health or sexuality reasons. IVPs are associated with adverse female health outcomes, development of bacterial vaginosis, HIV acquisition and transmission. A mixed methods approach was used in this study to examine the prevalence of IVP, assess reasons for engagement, and perceptions of IVP among a sample of minority (African-American and Hispanic) women infected, or at-risk, for HIV in Miami, USA, a city with increasing numbers of sexually transmitted infections (STIs) and HIV...
2017: International Journal of Women's Health
https://www.readbyqxmd.com/read/28280032/complete-genome-sequence-of-lactobacillus-jensenii-strain-snuv360-a-probiotic-for-treatment-of-bacterial-vaginosis-isolated-from-the-vagina-of-a-healthy-korean-woman
#3
Sunghee Lee, Hyun Ju You, Bomi Kwon, GwangPyo Ko
Lactobacillus jensenii SNUV360 is a potential probiotic strain that shows antimicrobial activity for the treatment of bacterial vaginosis. Here, we present the complete genomic sequence of L. jensenii SNUV360, isolated from a vaginal sample from a healthy Korean woman. Analysis of the sequence may provide insight into its functional activity.
March 9, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28278277/women-view-key-sexual-behaviours-as-the-trigger-for-the-onset-and-recurrence-of-bacterial-vaginosis
#4
Jade E Bilardi, Sandra M Walker, Meredith J Temple-Smith, Ruth P McNair, Julie Mooney-Somers, Lenka A Vodstrcil, Clare E Bellhouse, Christopher K Fairley, Catriona S Bradshaw
BACKGROUND: Bacterial vaginosis (BV) affects a third of women of reproductive age in the US and there is increasing evidence to suggest it may be sexually transmitted. This study aimed to extend and validate the findings of our earlier smaller qualitative study by exploring in detail women's views and experiences of the triggering factors associated with BV onset and recurrence. METHODS: Women aged 20-49, who had experienced one or more symptomatic episode of BV within 6 months, were opportunistically recruited to complete a 38-item questionnaire on their experience of BV...
2017: PloS One
https://www.readbyqxmd.com/read/28267861/periodic-screening-pelvic-examination-evidence-report-and-systematic-review-for-the-us-preventive-services-task-force
#5
Janelle M Guirguis-Blake, Jillian T Henderson, Leslie A Perdue
Importance: Recent changes in the periodicity of cervical cancer screening have led to questions about the role of screening pelvic examinations among asymptomatic women. Objective: To systematically review literature on health benefits, accuracy, and harms of the screening pelvic examination for gynecologic conditions for the US Preventive Services Task Force (USPSTF). Data Sources: MEDLINE, PubMed, and Cochrane Central Register of Controlled Trials for relevant English-language studies published through January 13, 2016, with surveillance through August 3, 2016...
March 7, 2017: JAMA: the Journal of the American Medical Association
https://www.readbyqxmd.com/read/28264668/implementing-and-sustaining-a-mobile-medical-clinic-for-prenatal-care-and-sexually-transmitted-infection-prevention-in-rural-mysore-india
#6
Noah Kojima, Karl Krupp, Kavitha Ravi, Savitha Gowda, Poornima Jaykrishna, Caitlyn Leonardson-Placek, Anand Siddhaiah, Claire C Bristow, Anjali Arun, Jeffrey D Klausner, Purnima Madhivanan
BACKGROUND: In rural India, mobile medical clinics are useful models for delivering health promotion, education, and care. Mobile medical clinics use fewer providers for larger catchment areas compared to traditional clinic models in resource limited settings, which is especially useful in areas with shortages of healthcare providers and a wide geographical distribution of patients. METHODS: From 2008 to 2011, we built infrastructure to implement a mobile clinic system to educate rural communities about maternal child health, train community health workers in common safe birthing procedures, and provide comprehensive antenatal care, prevention of mother-to-child transmission (PMTCT) of human immunodeficiency virus (HIV), and testing for specific infections in a large rural catchment area of pregnant women in rural Mysore...
March 6, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28263902/mollicutes-in-vaginal-microbiology-mycoplasma-hominis-ureaplasma-urealyticum-ureaplasma-parvum-and-mycoplasma-genitalium
#7
David Taylor-Robinson
Mycoplasma hominis was isolated in 1937 from the human genital tract, followed 17 years later by Ureaplasma urealyticum and 27 years after that by Mycoplasma genitalium. The first two proved relatively easy to culture but the latter required a polymerase chain reaction assay for further studies. In sexually mature women, M. hominis may be found in the vagina/cervix of about 20-50%, ureaplasmas in 40-80% and M. genitalium in 0-5%. Some heterogeneity has been found among strains of all these species, sufficient to divide ureaplasmas into two species, namely U...
March 2, 2017: Research in Microbiology
https://www.readbyqxmd.com/read/28262922/experts-explore-the-state-of-bacterial-vaginosis-and-the-unmet-needs-facing-women-and-providers
#8
Steven E Chavoustie, Scott E Eder, William D Koltun, Tracey R Lemon, Caroline Mitchell, Paul Nyirjesy, Jack D Sobel, Ryan Sobel, Rachel Villanueva
No abstract text is available yet for this article.
March 6, 2017: International Journal of Gynaecology and Obstetrics
https://www.readbyqxmd.com/read/28260585/comparison-of-culture-dependent-and-culture-independent-molecular-methods-for-characterization-of-vaginal-microflora
#9
COMPARATIVE STUDY
Shirali Pandya, Kavitha Ravi, Vijaya Srinivas, Smitha Jadhav, Anisa Khan, Anjali Arun, Lee W Riley, Purnima Madhivanan
PURPOSE: To date, molecular methods that circumvent the limitations of traditional culture methods have not been used to describe the vaginal microflora in India. Here, we compared culture and culture-independent molecular methods in characterizing the vaginal microbiota in Indian women. METHODOLOGY: Culture methods involved traditional cultivation on Rogosa and sheep blood agar, whereas culture-independent methods bypassed a culturing step by performing broadrange 16S rDNA PCR on DNA isolated directly from vaginal swabs...
March 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28257809/the-global-health-impact-of-vaginal-dysbiosis
#10
Janneke H H M van de Wijgert, Vicky Jespers
The most common dysbiosis of the vaginal microbiome (defined here as a vaginal microbiome not dominated by lactobacilli) is bacterial vaginosis, an anaerobic polybacterial dysbiosis. Other dysbiotic states of importance to global health are vaginal microbiota with a high abundance of streptococci, staphylococci or Enterobacteriaceae, vaginal candidiasis and trichomoniasis. Knowledge about the different types of dysbiosis and their relationship to urogenital and reproductive disease burden has increased in recent years by applying non-culture-based techniques, but is far from complete...
March 1, 2017: Research in Microbiology
https://www.readbyqxmd.com/read/28257539/sexually-transmitted-infection-risk-among-women-is-not-fully-explained-by-partner-numbers
#11
Christina A Muzny, Hanne S Harbison, Erika L Austin, Jane R Schwebke, Barbara Van Der Pol, Edward W Hook
OBJECTIVES: Increased sexual partner numbers may contribute to sexually transmitted infection (STI) risk for some but not all women. This study compared women reporting having four or more partners during the preceding year (multiple partnership group) with those reporting having one partner (single partnership group) with regard to sexually risky behaviors and other modifiable predictors of STI risk. We also compared the prevalence of bacterial vaginosis, trichomoniasis, chlamydia, gonorrhea, syphilis, herpes simplex virus type 2, and human immunodeficiency virus among groups...
March 2017: Southern Medical Journal
https://www.readbyqxmd.com/read/28254220/preliminary-preventive-protocol-from-first-trimester-of-pregnancy-to-reduce-preterm-birth-rate-for-dichorionic-diamniotic-twins
#12
Masako Matsui, Yuichiro Takahashi, Shigenori Iwagaki, Rika Chiaki, Kazuhiko Asai, Ichiro Kawabata
OBJECTIVE: The preterm birth rate of twins is reportedly higher than that of single pregnancies. We performed preliminary preventive interventions at our center focused on evaluating the risk of each case before 14 weeks of gestation to reduce the spontaneous preterm birth rate. MATERIALS AND METHODS: The participants included 184 dichorionic-diamniotic twins delivered at our center during the 8 years from 2006. We evaluated each patient regarding high-risk status (at least 1 additional factor as follows: threatened abortion, history of chorioamnionitis, cervicitis, and bacterial vaginosis), based on available evidence; patients deemed high risk gave their informed consent and underwent treatment for cervicitis and cerclage if indicated...
February 2017: Taiwanese Journal of Obstetrics & Gynecology
https://www.readbyqxmd.com/read/28241058/the-presence-of-the-putative-gardnerella-vaginalis-sialidase-a-gene-in-vaginal-specimens-is-associated-with-bacterial-vaginosis-biofilm
#13
Liselotte Hardy, Vicky Jespers, Magelien Van den Bulck, Jozefien Buyze, Lambert Mwambarangwe, Viateur Musengamana, Mario Vaneechoutte, Tania Crucitti
Bacterial vaginosis (BV) is a difficult-to-treat recurrent condition in which health-associated lactobacilli are outnumbered by other anaerobic bacteria, such as Gardnerella vaginalis. Certain genotypes of G. vaginalis can produce sialidase, while others cannot. Sialidase is known to facilitate the destruction of the protective mucus layer on the vaginal epithelium by hydrolysis of sialic acid on the glycans of mucous membranes. This process possibly facilitates adhesion of bacterial cells on the epithelium since it has been linked with the development of biofilm in other pathogenic conditions...
2017: PloS One
https://www.readbyqxmd.com/read/28238844/bacterial-species-colonizing-the-vagina-of-healthy-women-are-not-associated-with-race
#14
May A Beamer, Michele N Austin, Hilary A Avolia, Leslie A Meyn, Katherine E Bunge, Sharon L Hillier
The vaginal microbiota of 36 white versus 25 black asymptomatic women were compared using both cultivation-dependent and -independent identification. Significant differences by race were found in colonization and density of bacterial species. However, exclusion of 12 women with bacterial vaginosis by Nugent criteria resulted in no significant differences by race.
February 24, 2017: Anaerobe
https://www.readbyqxmd.com/read/28223255/olegusella-massiliensis-gen-nov-sp-nov-strain-khd7-t-a-new-bacterial-genus-isolated-from-the-female-genital-tract-of-a-patient-with-bacterial-vaginosis
#15
Khoudia Diop, Awa Diop, Florence Bretelle, Frédéric Cadoret, Caroline Michelle, Magali Richez, Jean-François Cocallemen, Didier Raoult, Pierre-Edouard Fournier, Florence Fenollar
Strain KHD7(T), a Gram-stain-positive rod-shaped, non-sporulating, strictly anaerobic bacterium, was isolated from the vaginal swab of a woman with bacterial vaginosis. We studied its phenotypic characteristics and sequenced its complete genome. The major fatty acids were C16:0 (44%), C18:2n6 (22%), and C18:1n9 (14%). The 1,806,744 bp long genome exhibited 49.24% G+C content; 1549 protein-coding and 51 RNA genes. Strain KHD7(T) exhibited a 93.5% 16S rRNA similarity with Olsenella uli, the phylogenetically closest species in the family Coriobacteriaceae...
February 20, 2017: Anaerobe
https://www.readbyqxmd.com/read/28210940/syndromic-diagnosis-in-evaluation-of-women-with-symptoms-of-vaginitis
#16
REVIEW
Theophilus Ogochukwu Nwankwo, Uzochukwu Uzoma Aniebue, Uchenna Anthony Umeh
PURPOSE OF REVIEW: This review aims to determine the effectiveness of the use of syndrome diagnosis in the evaluation of vaginitis and to make suggestions based on the review findings. RECENT FINDINGS: Vaginal discharge as the main symptom of vaginitis is unspecific. A randomized study of symptom-based diagnosis and treatment of vaginitis in the USA favored symptoms used for treatment; however, this was only a pilot study. Hence, a population-based study is necessary to validate these findings...
January 2017: Current Infectious Disease Reports
https://www.readbyqxmd.com/read/28208268/medical-swab-analysis-using-desorption-electrospray-ionization-mass-spectrometry-a-noninvasive-approach-for-mucosal-diagnostics
#17
Pamela Pruski, David A MacIntyre, Holly V Lewis, Paolo Inglese, Gonçalo D S Correia, Trevor T Hansel, Phillip R Bennett, Elaine Holmes, Zoltan Takats
Medical swabs are routinely used worldwide to sample human mucosa for microbiological screening with culture methods. These are usually time-consuming and have a narrow focus on screening for particular microorganism species. As an alternative, direct mass spectrometric profiling of the mucosal metabolome provides a broader window into the mucosal ecosystem. We present for the first time a minimal effort/minimal-disruption technique for augmenting the information obtained from clinical swab analysis with mucosal metabolome profiling using desorption electrospray ionization mass spectrometry (DESI-MS) analysis...
February 7, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28197729/gardnerella-vaginalis-population-dynamics-in-bacterial-vaginosis
#18
D W Hilbert, J A Schuyler, M E Adelson, E Mordechai, J D Sobel, S E Gygax
Bacterial vaginosis (BV) is the leading cause of vaginal discharge and is associated with the facultative Gram-variable bacterium Gardnerella vaginalis, whose population structure consists of four clades. Our goal was to determine if these clades differ with regard to abundance during BV. We performed a short-term longitudinal study of BV. Patients were evaluated according to the Amsel criteria and Nugent scoring at initial diagnosis, immediately after treatment and at a 40- to 45-day follow-up visit. G. vaginalis clade abundance was determined by quantitative real-time polymerase chain reactions (qPCRs)...
February 14, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28197138/genetic-and-phenotypic-screening-of-mannose-binding-lectin-in-relation-to-risk-of-recurrent-vulvovaginal-infections-in-women-of-north-india-a-prospective-cohort-study
#19
Namarta Kalia, Jatinder Singh, Sujata Sharma, Hardesh Arora, Manpreet Kaur
Recurrent Vulvovaginal Infections (RVVI) is common problem associated with women of reproductive age. The function and deleterious effect of Mannose Binding Lectin 2 (MBL2) common polymorphisms are reported to be associated with various diseases. However, the role of MBL2 promoter gene polymorphisms and their combined effect with structural variant along with Serum Mannose Binding Lectin (sMBL) levels in RVVI has not been investigated. The study included 258 RVVI cases and 203 age matched healthy controls. These were investigated for the distribution of MBL2 codon 54 and promoter polymorphisms by Amplification Refractory Mutation System-Polymerase Chain Reaction (ARMS-PCR)...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28163330/in-non-pregnant-patients-over-the-age-of-18-with-symptomatic-bacterial-vaginosis-does-the-addition-of-oral-or-vaginal-probiotics-compared-to-standard-antibiotic-use-alone-decrease-time-to-recurrence
#20
Melissa Shanay Herring, Jeffery Dean Hodgden
No abstract text is available yet for this article.
October 2016: Journal of the Oklahoma State Medical Association
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