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https://www.readbyqxmd.com/read/29455132/rapid-sewage-pollution-assessment-by-means-of-the-coverage-of-epilithic-taxa-in-a-coastal-area-in-the-sw-atlantic
#1
M E Becherucci, M L Jaubet, M A Saracho Bottero, E N Llanos, R Elías, G V Garaffo
The sewage pollution impact over coastal environment represents one of the main reasons explaining the deterioration of marine coastal ecosystems around the globe. This paper aims to detect promptly a putative sewage pollution impact in a Southwestern Atlantic coastal area of Argentina as well as to identify a straightforward way for monitoring, based on the relative abundance coverage of the intertidal epilithic taxa. Four sampling sites were distributed at increased distances from the sewage outfall where the cover of individual epilithic species was visually estimated...
February 15, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/29452934/evaluation-of-leader-peptides-that-affect-the-secretory-ability-of-a-multiple-bacteriocin-transporter-enkt
#2
Hirotoshi Sushida, Naoki Ishibashi, Takeshi Zendo, Pongtep Wilaipun, Vichien Leelawatcharamas, Jiro Nakayama, Kenji Sonomoto
EnkT is a novel ATP-binding cassette (ABC) transporter responsible for secretion of four bacteriocins, enterocins NKR-5-3A, C, D, and Z (Ent53A, C, D, and Z), produced by Enterococcus faecium NKR-5-3. It is generally recognized that the secretion of a bacteriocin requires a dedicated ABC transporter, although molecular mechanisms of this secretion are yet to be revealed. In order to characterize the unique ability of EnkT to secrete multiple bacteriocins, the role of N-terminal leader peptides of bacteriocin precursors was evaluated using Ent53C precursor as a model...
February 13, 2018: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/29451651/demetallization-of-enterococcus-faecalis-biofilm-a-preliminary-study
#3
Carlos Estrela, Rodrigo Costa E Silva, Roberta Cerasi Urban, Pablo José Gonçalves, Júlio A Silva, Cyntia R A Estrela, Jesus Djalma Pecora, Ove A Peters
Objectives To determine the concentration of calcium, iron, manganese and zinc ions after the application of chelator to Enterococcus faecalis biofilms. Material and Methods Fifty bovine maxillary central incisors were prepared and inoculated with E. faecalis for 60 days. The following were used as irrigation solutions: 17% EDTA (pH 3, 7 and 10), 2.5% sodium hypochlorite (NaOCl) combined with 17% EDTA (pH 3, 7 and 10), distilled water (pH 3, 7 and 10), and 2.5% NaOCl. Each solution was kept in the root canal for five minutes...
2018: Journal of Applied Oral Science: Revista FOB
https://www.readbyqxmd.com/read/29449385/complete-genome-sequences-of-enterobacter-cancerogenus-cr-eb1-and-enterococcus-sp-strain-cr-ec1-isolated-from-the-larval-gut-of-the-greater-wax-moth-galleria-mellonella
#4
Joon-Hui Chung, Haeyoung Jeong, Choong-Min Ryu
Enterobacter cancerogenus CR-Eb1 and Enterococcus sp. CR-Ec1 were isolated from the larval gut of Galleria mellonella , the greater wax moth. Here, we report the completed and annotated genome sequences of insect gut-dwelling bacteria.
February 15, 2018: Genome Announcements
https://www.readbyqxmd.com/read/29449171/changes-in-antimicrobial-susceptibility-of-commonly-clinically-significant-isolates-before-and-after-the-interventions-on-surgical-prophylactic-antibiotics-spas-in-shanghai
#5
Su Wang, Li-Zhong Han, Yu-Xing Ni, Yi-Bo Zhang, Qun Wang, Da-Ke Shi, Wen-Hui Li, Yi-Chen Wang, Chen-Rong Mi
Surveillances and interventions on antibiotics use have been suggested to improve serious drug-resistance worldwide. Since 2007, our hospital have proposed many measures for regulating surgical prophylactic antibiotics (carbapenems, third gen. cephalosporins, vancomycin, etc.) prescribing practices, like formulary restriction or replacement for surgical prophylactic antibiotics and timely feedback. To assess the impacts on drug-resistance after interventions, we enrolled infected patients in 2006 (pre-intervention period) and 2014 (post-intervention period) in a tertiary hospital in Shanghai...
February 12, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29449095/relationships-between-bacteriospermia-dna-integrity-nuclear-protamine-alteration-sperm-quality-and-icsi-outcome
#6
Ali Zeyad, Mohammed Hamad, Houda Amor, Mohamad Eid Hammadeh
The Aim of this study was to evaluate the effects of bacteriospermia on human sperm parameters, nuclear protamines, DNA integrity and ICSI outcome in patients enrolled for ICSI treatment. 84 unselected couples consulting in infertility and obstetrics clinic and enrolled for ICSI treatment were included in this study. The semen specimens were screened bacteriologically; semen and sperm parameters were also evaluated according to WHO guidelines. DNA integrity, protamines concentration and protamine deficiency were estimated by TUNEL assay, AU-PAGE and Chromomycin (CMA3) respectively...
February 12, 2018: Reproductive Biology
https://www.readbyqxmd.com/read/29446685/inactivation-of-salmonella-and-surrogate-bacteria-on-cashews-and-macadamia-nuts-exposed-to-commercial-propylene-oxide-processing-conditions
#7
Thomas Saunders, Jian Wu, Robert C Williams, Haibo Huang, Monica A Ponder
Propylene oxide (PPO), a chemical fumigant, has been validated to reduce Salmonella on bulk almonds but has not been evaluated for other tree nuts. There is a need to identify nonpathogenic surrogate microorganisms whose inactivation is comparable to that of Salmonella to assure effective PPO processing parameters in different packaging configurations without introducing Salmonella into the pasteurization facility. The objective of this research was to compare the reduction of Salmonella and three potential surrogate bacterial strains, Enterococcus faecium ATCC 8459, Pediococcus acidilactici ATCC 8042, or Staphylococcus carnosus ATCC 51365, on cashews and macadamia nuts processed by using PPO...
February 15, 2018: Journal of Food Protection
https://www.readbyqxmd.com/read/29446057/in-vitro-evaluation-of-probiotic-potential-of-lactic-acid-bacteria-isolated-from-yunnan-de-ang-pickled-tea
#8
Zhenhui Cao, Hongbin Pan, Shijun Li, Chongying Shi, Sifan Wang, Fuyi Wang, Pengfei Ye, Junjing Jia, Changrong Ge, Qiuye Lin, Zhiyong Zhao
This study aimed to investigate the probiotic potential of lactic acid bacteria (LAB) strains isolated from De'ang pickled tea, a traditional food consumed by the De'ang nationality of Yunnan, China. Twenty-six LAB strains isolated from De'ang pickled tea were subjected to identification based on 16S rRNA gene sequence analysis. Twenty-four belonged to Lactobacillus plantarum, one belonged to Enterococcus casseliflavus, and one belonged to Lactobacillus acidophilus. Eighteen out of 26 LAB strains which showed a higher capability to tolerate simulated gastrointestinal juices were chosen to further evaluate their probiotic properties...
February 14, 2018: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/29445637/virulence-determinants-and-antimicrobial-resistance-patterns-of-vancomycin-resistant-enterococcus-faecium-isolated-from-different-sources-in-southwest-iran
#9
Maniya Arshadi, Mahmood Mahmoudi, Moloud Sadat Motahar, Saber Soltani, Mohammad Reza Pourmand
Background: This study aimed to investigate the incidence of antibiotic-resistance and virulence genes in vancomycin-resistant Enterococcus faecium isolated from different sources in southwest Iran from Mar to Sep 2015. Methods: Overall, 120 E. faecium isolates (80 VRE and 40 vancomycin-susceptible enterococci [VSE] isolates) were obtained from four hospitals. The resistance of the VRE isolates was determined by disk diffusion method. Multiplex PCR was performed to detect the virulence genes carried by the E...
February 2018: Iranian Journal of Public Health
https://www.readbyqxmd.com/read/29443786/improved-vacuum-sealing-drainage-for-treatment-of-surgical-site-infection-following-posterior-spinal-internal-fixation-a-case-report
#10
Hengyan Zhang, Qiyi Li
RATIONALE: Surgical site infection (SSI) following spine surgeries involving internal fixation often require removing the instrument; however, this can cause spinal instability. Previous reports have demonstrated the usefulness of vacuum sealing drainage (VSD) therapy, but the cases require wound opening, aseptic condition to replace the VSD device, and a secondary operation to close the wound. Thus, to improve the VSD treatment and develop a maneuverable procedure, make sense in spine surgery...
February 2018: Medicine (Baltimore)
https://www.readbyqxmd.com/read/29443752/the-first-case-report-of-enterococcus-gallinarum-meningitis-in-neonate-a-literature-review
#11
Xiaoquan Li, Shujuan Fan, Xiaojie Lin, Li Liu, Jie Zheng, Xihui Zhou, Axel Heep
RATIONALE: Enterococcus gallinarum meningitis (EGM) is rarely found in normal adults and even rarer in children. To our knowledge, EGM in neonate has not been reported previously. PATIENTS CONCERNS: Here we reported the first case of EGM in neonate. Prolonged fever was the only manifestation for the case after admission. DIAGNOSES: Cerebrospinal fluid cultures showed that the isolate was Enterococcus gallinarum and sensitive to linezolid. INTERVENTIONS: Ceftriaxone, beta lactam type, and vancomycin were used respectively, but not effective...
February 2018: Medicine (Baltimore)
https://www.readbyqxmd.com/read/29442195/the-changing-epidemiology-of-vanb-enterococcus-faecium-in-poland
#12
Ewa Sadowy, Iwona Gawryszewska, Alicja Kuch, Dorota Żabicka, Waleria Hryniewicz
Increasing prevalence of VanB Enterococcus faecium in Polish hospitals reported to National Reference Centre for Susceptibility Testing (NRCST) prompted us to investigate the basis of this phenomenon. Two-hundred seventy-eight E. faecium isolates of VanB phenotype from the period 1999 to 2010 obtained by NRCST were investigated by multilocus sequence typing (MLST) and multilocus VNTR analysis (MLVA). Localization, transferability, and partial structure of the vanB-carrying Tn1549 transposon were studied by hybridization, PCR mapping, sequencing, and conjugation...
February 13, 2018: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/29441802/phage-derived-lysins-as-therapeutic-agents-against-multidrug-resistant-enterococcus-faecalis
#13
Jingmin Gu, Hengyu Xi, Mengjun Cheng, Wenyu Han
No abstract text is available yet for this article.
February 14, 2018: Future Microbiology
https://www.readbyqxmd.com/read/29438718/safety-potential-biotechnological-and-probiotic-properties-of-bacteriocinogenic-enterococcus-lactis-strains-isolated-from-raw-shrimps
#14
Olfa Ben Braïek, Stefano Morandi, Paola Cremonesi, Slim Smaoui, Khaled Hani, Taoufik Ghrairi
The aims of this study are to isolate new bacteriocinogenic lactic acid bacterial strains from white (Penaeus vannamei) and pink (Palaemon serratus) raw shrimps and evaluate their technological and probiotic potentialities. Seven strains were selected, among fifty active isolates, as producing interesting antimicrobial activity. Identified as Enterococcus lactis, these isolates were able to produce enterocins A, B and/or P. The safety aspect, assessed by microbiological and molecular tests, demonstrated that the strains were susceptible to relevant antibiotics such as vancomycin, negative for haemolysin and gelatinase activities, and did not harbour virulence and antibiotic resistance genes...
February 10, 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29438479/novel-tn916-like-elements-confer-aminoglycoside-macrolide-co-resistance-in-clinical-isolates-of-streptococcus-gallolyticus-ssp-gallolyticus
#15
Stanimir Kambarev, Frédéric Pecorari, Stéphane Corvec
Background: Streptococcus gallolyticus ssp. gallolyticus (Sgg) is a commensal bacterium and an opportunistic pathogen. In humans it has been clinically associated with the incidence of colorectal cancer (CRC) and epidemiologically recognized as an emerging cause of infective endocarditis (IE). The standard therapy of Sgg includes the administration of a penicillin in combination with an aminoglycoside. Even though penicillin-resistant isolates have still not been reported, epidemiological studies have shown that this microbe is a reservoir of multiple acquired genes, conferring resistance to tetracyclines, aminoglycosides, macrolides and glycopeptides...
February 9, 2018: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29436902/both-live-and-dead-enterococci-activate-caenorhabditis-elegans-host-defense-via-immune-and-stress-pathways
#16
Grace J Yuen, Frederick M Ausubel
The innate immune response of the nematode Caenorhabditis elegans has been extensively studied and a variety of Toll-independent immune response pathways have been identified. Surprisingly little, however, is known about how pathogens activate the C. elegans immune response. Enterococcus faecalis and Enterococcus faecium are closely related enterococcal species that exhibit significantly different levels of virulence in C. elegans infection models. Previous work has shown that activation of the C. elegans immune response by Pseudomonas aeruginosa involves P...
February 13, 2018: Virulence
https://www.readbyqxmd.com/read/29436593/l%C3%A2-histidine-augments-the-oxidative-damage-against-gram%C3%A2-negative-bacteria-by-hydrogen-peroxide
#17
Tamiko Nagao, Haruyuki Nakayama-Imaohji, Miad Elahi, Ayano Tada, Emika Toyonaga, Hisashi Yamasaki, Katsuichiro Okazaki, Hirokazu Miyoshi, Koichiro Tsuchiya, Tomomi Kuwahara
Excessive damage to DNA and lipid membranes by reactive oxygen species reduces the viability of bacteria. In the present study, the proliferation of recA‑deficient Escherichia coli strains was revealed to be inhibited by 1% L‑histidine under aerobic conditions. This inhibition of proliferation was not observed under anaerobic conditions, indicating that L‑histidine enhances oxidative DNA damage to E. coli cells. Reverse transcription‑quantitative polymerase chain reaction analysis demonstrated that the expression of recA in E...
February 7, 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29434698/effect-of-clostridium-butyricum-supplementation-on-the-development-of-intestinal-flora-and-the-immune-system-of-neonatal-mice
#18
Rui-Xue Miao, Xin-Xin Zhu, Chao-Min Wan, Zhi-Ling Wang, Yang Wen, Yi-Yuan Li
The objective of the present study was to examine whether Clostridium butyricum supplementation has a role in the regulation of the intestinal flora and the development of the immune system of neonatal mice. A total of 30 pregnant BALB/c mice, including their offspring, were randomly divided into three groups: In the maternal intervention group (Ba), maternal mice were treated with Clostridium butyricum from birth until weaning at postnatal day 21 (PD21) followed by administration of saline to the offspring at PD21-28; in the offspring intervention group (Ab), breast-feeding maternal mice were supplemented with saline and offspring were directly supplemented with Clostridium butyricum from PD21-28; in the both maternal and offspring intervention group (Bb), both maternal mice and offspring were supplemented with Clostridium butyricum at PD 0-21 and at PD21-28...
January 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29432912/in-vitro-evaluation-of-new-2-phenoxy-benzo-g-1-2-4-triazolo-1-5-a-quinazoline-derivatives-as-antimicrobial-agents
#19
Hatem A Abuelizz, Rabab A El-Dib, Mohamed Marzouk, Rashad Al-Salahi
Previously, seventeen 2-phenoxy-benzo[g][1,2,4]triazolo[1,5-a]quinazoline derivatives were prepared and characterized by physicochemical and spectral means. This study was conducted to evaluate their activities in vitro against five Gram-negative and five Gram-positive of clinically pathogenic bacterial strains and ten fungal strains. The antimicrobial activity was assessed, and the minimum inhibitory concentration values of the tested compounds were determined in μg ml-1 , using the diffusion agar technique...
February 9, 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29432868/repurposing-niclosamide-for-intestinal-decolonization-of-vancomycin-resistant-enterococci
#20
Haroon Mohammad, Ahmed AbdelKhalek, Nader S Abutaleb, Mohamed N Seleem
Enterococci are commensal microorganisms present in the gastrointestinal (GI) tract of humans. Though normally innocuous to the host, strains of Enterococcus exhibiting resistance to vancomycin (VRE) have been associated with high rates of infection and mortality in immunocompromised patients. Decolonization of VRE represents a key strategy to curb infection in highly-susceptible patients. However, there is a dearth of decolonizing agents available clinically that are effective against VRE. The present study confirms that niclosamide, an anthelmintic drug, has potent antibacterial activity against clinical isolates of vancomycin-resistant E...
February 9, 2018: International Journal of Antimicrobial Agents
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