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https://www.readbyqxmd.com/read/28221912/isolation-identification-and-evaluation-of-new-lactic-acid-bacteria-strains-with-both-cellular-antioxidant-and-bile-salt-hydrolase-activities-in-vitro
#1
Shuang Xu, Taigang Liu, Chiraz Akorede Ibinke Radji, Jing Yang, Lanming Chen
In this study, we analyzed Chinese traditional fermented food to isolate and identify new lactic acid bacteria (LAB) strains with novel functional properties and to evaluate their cellular antioxidant and bile salt hydrolase (BSH) activities in vitro. A sequential screening strategy was developed to efficiently isolate and obtain 261 LAB strains tolerant of bile salt, acid, and H2O2 from nine Chinese traditional fermented foods. Among these strains, 70 were identified as having 2,2-diphenyl-1-picrylhydrazyl radical scavenging and/or BSH activity...
November 2016: Journal of Food Protection
https://www.readbyqxmd.com/read/28221890/enhanced-control-of-listeria-monocytogenes-by-enterococcus-faecium-ke82-a-multiple-enterocin-producing-strain-in-different-milk-environments
#2
Elpiniki Vandera, Alexandra Lianou, Athanasia Kakouri, Jinbo Feng, Anna-Irini Koukkou, John Samelis
Enterococcus faecium KE82, isolated from traditional Greek Graviera cheese, was identified in pure broth cultures in vitro as a multiple enterocin-producing bacterial strain possessing the structural entA, entB, and entP enterocin genes. E. faecium KE82 was further assessed for in situ antilisterial activity in raw milk (RM) and commercially thermized milk (TM; 63°C for 30 s) in the presence of the indigenous microbiota and in sterile raw milk (SRM; 121°C for 5 min) with or without the addition of two commercial starter culture (CSC) strains Streptococcus thermophilus and Lactococcus lactis ...
January 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28221889/inactivation-of-gram-positive-bacteria-by-novel-phenolic-branched-chain-fatty-acids
#3
Xuetong Fan, Karen Wagner, Kimberly J B Sokorai, Helen Ngo
Novel phenolic branched-chain fatty acids (PBC-FAs) were evaluated for their antimicrobial properties against both gram-positive ( Listeria innocua , Bacillus subtilis , Enterococcus faecium ) and gram-negative ( Escherichia coli , Salmonella Typhimurium, and Pseudomonas tolaasii ) bacteria. In addition, PBC-FA derivatives, such as PBC-FA methyl ester mixture, methyl-branched fatty acid mixtures, and trimethylsilyl-PBC-FA methyl esters, were synthesized to study the structure activity relationship. Results showed that PBC-FAs were a potent antimicrobial against gram-positive bacteria with MICs of 1...
January 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28221886/technological-properties-and-biogenic-amines-production-by-bacteriocinogenic-lactococci-and-enterococci-strains-isolated-from-raw-goat-s-milk
#4
Luana Martins Perin, Simona Belviso, Barbara Dal Bello, Luís Augusto Nero, Luca Cocolin
Technological properties and biogenic amine production were analyzed in 56 bacteriocinogenic lactococci and enterococci strains isolated from raw goat's milk. Fifteen lactococci strains were able to reduce milk pH to 5.3 or lower after 6 h, while enterococci strains were initially slow in producing acids. Lactococcus lactis subsp. lactis GLc06 and three strains of Enterococcus faecalis (GEn20, GEn22, and GEn23) presented high proteolytic activity. L. lactis subsp. lactis GLc06 and E. faecalis GEn22 also showed a high percentage of autolysis after only 4 h, reaching 71...
January 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28221874/comparing-thermal-process-validation-methods-for-salmonella-inactivation-on-almond-kernels
#5
Sanghyup Jeong, Bradley P Marks, Michael K James
Ongoing regulatory changes are increasing the need for reliable process validation methods for pathogen reduction processes involving low-moisture products; however, the reliability of various validation methods has not been evaluated. Therefore, the objective was to quantify accuracy and repeatability of four validation methods (two biologically based and two based on time-temperature models) for thermal pasteurization of almonds. Almond kernels were inoculated with Salmonella Enteritidis phage type 30 or Enterococcus faecium (NRRL B-2354) at ~10(8) CFU/g, equilibrated to 0...
January 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28219792/conjugative-type-iv-secretion-in-gram-positive-pathogens-trag-a-lytic-transglycosylase-and-endopeptidase-interacts-with-translocation-channel-protein-tram
#6
Verena Kohler, Ines Probst, Andreas Aufschnaiter, Sabrina Büttner, Lisa Schaden, Gerald Rechberger, Günther Koraimann, Elisabeth Grohmann, Walter Keller
Conjugative transfer plays a major role in the transmission of antibiotic resistance in bacteria. pIP501 is a Gram-positive conjugative model plasmid with the broadest transfer host-range known so far and is frequently found in Enterococcus faecalis and Enterococcus faecium clinical isolates. The pIP501 type IV secretion system is encoded by 15 transfer genes. In this work, we focus on the VirB1-like protein TraG, a modular peptidoglycan metabolizing enzyme, and the VirB8-homolog TraM, a potential member of the translocation channel...
February 17, 2017: Plasmid
https://www.readbyqxmd.com/read/28219141/-significance-of-bacteria-detection-with-filter-paper-method-on-diagnosis-of-diabetic-foot-wound-infection
#7
X H Zou, Y P Zhu, G Q Ren, G C Li, J Zhang, L J Zou, Z B Feng, B H Li
Objective: To evaluate the significance of bacteria detection with filter paper method on diagnosis of diabetic foot wound infection. Methods: Eighteen patients with diabetic foot ulcer conforming to the study criteria were hospitalized in Liyuan Hospital Affiliated to Tongji Medical College of Huazhong University of Science and Technology from July 2014 to July 2015. Diabetic foot ulcer wounds were classified according to the University of Texas diabetic foot classification (hereinafter referred to as Texas grade) system, and general condition of patients with wounds in different Texas grade was compared...
February 20, 2017: Zhonghua Shao Shang za Zhi, Zhonghua Shaoshang Zazhi, Chinese Journal of Burns
https://www.readbyqxmd.com/read/28216060/incidence-of-fever-in-labor-and-risk-of-neonatal-sepsis
#8
Craig V Towers, Angela Yates, Nikki Zite, Casey Smith, Lindsey Chernicky, Bobby Howard
BACKGROUND: The current recommendation regarding the management of a term newborn delivered of a mother with an intrapartum fever or a diagnosis of clinical chorioamnionitis is that the neonate should have baseline laboratory work drawn along with blood cultures and be universally treated with antibiotics until culture results return. These guidelines report that the rate of intrapartum fever is about 3%; however, a few large studies suggest that the rate is higher at about 7%. OBJECTIVE: To prospectively evaluate the rate of fever during labor in a large number of deliveries and determine the rate of early onset neonatal sepsis in newborns delivered from mothers with an intrapartum fever compared with the newborns that delivered from mothers that did not have an intrapartum fever...
February 16, 2017: American Journal of Obstetrics and Gynecology
https://www.readbyqxmd.com/read/28213019/growth-interactions-and-antilisterial-effects-of-the-bacteriocinogenic-lactococcus-lactis-subsp-cremoris-m104-and-enterococcus-faecium-ke82-strains-in-thermized-milk-in-the-presence-or-absence-of-a-commercial-starter-culture
#9
Alexandra Lianou, Athanasia Kakouri, Eleni C Pappa, John Samelis
Traditional Greek cheeses are often produced from thermized milk (TM) with the use of commercial starter cultures (CSCs), which may not inhibit growth of Listeria monocytogenes completely. Therefore, this study evaluated the behavior of an artificial L. monocytogenes contamination in commercially TM (63 °C; 30 s) inoculated with a CSC plus Lactococcus lactis subsp. lactis M104 and/or Enterococcus faecium KE82, two indigenous strains producing nisin A and enterocin A and B, respectively. Inoculation treatments included TM with the CSC only, and TM without the CSC but with strain M104 alone, or combined with strain KE82...
June 2017: Food Microbiology
https://www.readbyqxmd.com/read/28210951/treatment-of-arctic-wastewater-by-chemical-coagulation-uv-and-peracetic-acid-disinfection
#10
Ravi Kumar Chhetri, Ewa Klupsch, Henrik Rasmus Andersen, Pernille Erland Jensen
Conventional wastewater treatment is challenging in the Arctic region due to the cold climate and scattered population. Thus, no wastewater treatment plant exists in Greenland, and raw wastewater is discharged directly to nearby waterbodies without treatment. We investigated the efficiency of physicochemical wastewater treatment, in Kangerlussuaq, Greenland. Raw wastewater from Kangerlussuaq was treated by chemical coagulation and UV disinfection. By applying 7.5 mg Al/L polyaluminium chloride (PAX XL100), 73% of turbidity and 28% phosphate was removed from raw wastewater...
February 16, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28209400/multidrug-resistant-enterococcal-infections-new-compounds-novel-antimicrobial-therapies
#11
REVIEW
Roel M van Harten, Rob J L Willems, Nathaniel I Martin, Antoni P A Hendrickx
Over the past two decades infections due to antibiotic-resistant bacteria have escalated world-wide, affecting patient morbidity, mortality, and health care costs. Among these bacteria, Enterococcus faecium and Enterococcus faecalis represent opportunistic nosocomial pathogens that cause difficult-to-treat infections because of intrinsic and acquired resistance to a plethora of antibiotics. In recent years, a number of novel antimicrobial compound classes have been discovered and developed that target Gram-positive bacteria, including E...
February 13, 2017: Trends in Microbiology
https://www.readbyqxmd.com/read/28207269/a-biocompatible-injectable-hydrogel-with-potent-wound-healing-and-antibacterial-properties
#12
Jiaul Hoque, Relekar G Prakash, Krishnamoorthy Paramanandham, Bibek R Shome, Jayanta Haldar
Two component injectable hydrogels that cross-link in-situ have been used as non-invasive wound-filling devices, i.e., sealants. These materials carry a variety of functions at the wound sites such as seal leaks, cease unwanted bleeding, bind tissues together, and assist in wound healing process. However, commonly used sealants typically lack antibacterial properties. Since bacterial infection at the wound site is very common, bioadhesive materials with intrinsic antibacterial properties are urgently required...
February 16, 2017: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/28204495/early-in-vitro-development-of-daptomycin-non-susceptibility-in-high-level-aminoglycoside-resistant-enterococcus-faecalis-predicts-the-efficacy-of-the-combination-of-high-dose-daptomycin-plus-ampicillin-in-an-in-vivo-model-of-experimental-endocarditis
#13
J M Pericàs, C García-de-la-Mària, M Brunet, Y Armero, J García-González, G Casals, M Almela, E Quintana, C Falces, S Ninot, D Fuster, J Llopis, F Marco, A Moreno, J M Miró
No abstract text is available yet for this article.
February 14, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28203378/screening-for-vancomycin-resistant-enterococci-with-xpert%C3%A2-vana-vanb-diagnostic-accuracy-and-impact-on-infection-control-decision-making
#14
B J Holzknecht, D S Hansen, L Nielsen, A Kailow, J O Jarløv
Vancomycin-resistant enterococci (VRE) are increasingly important nosocomial pathogens and screening for colonization status is a mainstay in infection control. We implemented PCR-based screening during vanA-positive Enterococcus faecium outbreaks in four university hospitals in Copenhagen, Denmark. Xpert(®)vanA/vanB was performed directly on rectal swabs and the vanA PCR result was used to guide infection control measures. Concurrently, all samples were selectively cultured including an overnight enrichment step...
March 2017: New Microbes and New Infections
https://www.readbyqxmd.com/read/28203232/cationic-net-charge-and-counter-ion-type-as-antimicrobial-activity-determinant-factors-of-short-lipopeptides
#15
Katarzyna E Greber, Malgorzata Dawgul, Wojciech Kamysz, Wieslaw Sawicki
To get a better insight into the antimicrobial potency of short cationic lipopeptides, 35 new entities were synthesized using solid phase peptide strategy. All newly obtained lipopeptides were designed to be positively charged from +1 to +4. This was achieved by introducing basic amino acid - lysine - into the lipopeptide structure and had a hydrophobic fatty acid chain attached. Lipopeptides were subjected to microbiological tests using reference strains of Gram-negative bacteria: Escherichia coli, Klebsiella pneumoniae, Proteus vulgaris, Pseudomonas aeruginosa, Gram-positive bacteria: Staphylococcus aureus, Staphylococcus epidermidis, Bacillus subtilis, Enterococcus faecalis, and fungi: Candida albicans, Candida tropicalis, Aspergillus brasiliensis...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28203227/some-technological-properties-of-lactic-acid-bacteria-isolated-from-dahi-and-datshi-naturally-fermented-milk-products-of-bhutan
#16
H N J Shangpliang, Sharmila Sharma, Ranjita Rai, Jyoti P Tamang
Dahi and datshi are common naturally fermented milk (NFM) products of Bhutan. Population of lactic acid bacteria (LAB) in dahi (pH 3.7) and datshi (pH 5.2) was 1.4 × 10(7) and 3.9 × 10(8) cfu/ml, respectively. Based on 16S rRNA gene sequencing isolates of LAB from dahi and datshi were identified as Enterococcus faecalis, E. faecium, Lactococcus lactis subsp. lactis. LAB strains were tested for some technological properties. All LAB strains except E. faecalis CH2:17 caused coagulation of milk at both 30°C for 48 h...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28202007/diffusible-substances-from-lactic-acid-bacterial-cultures-exert-strong-inhibitory-effects-on-listeria-monocytogenes-and-salmonella-enterica-serovar-enteritidis-in-a-co-culture-model
#17
Solomon H Mariam, Nigus Zegeye, Abraham Aseffa, Rawleigh Howe
BACKGROUND: Food-borne infections cause huge economic and human life losses. Listeria monocytogenes and Salmonella enterica serovar Enteritidis are among the top ranking pathogens causing such losses. Control of such infections is hampered by persistent contamination of foods and food-processing environments, resistance of pathogens to sanitizing agents, existence of heterogeneous populations of pathogens (including culturable and viable but non-culturable cells) within the same food items, and inability to detect all such pathogens by culture-based methods...
February 15, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28201936/effects-of-lactobacillus-casei-and-enterococcus-faecalis-on-growth-performance-immune-function-and-gut-microbiota-of-suckling-piglets
#18
Chaoqi Liu, Qun Zhu, Juan Chang, Qingqiang Yin, Andong Song, Zhentian Li, Erzhu Wang, Fushan Lu
This study was carried out to investigate the effects of orally administrated Lactobacillus casei and Enterococcus faecalis on performance, immune function and gut microbiota of suckling piglets. Neonatal piglets (n = 120) were randomly assigned to 4 groups, with 30 suckling piglets in each group. The piglets were from 15 litters, one male and one female piglet were selected for each group in each litter. The Control group was administrated with normal saline, the other groups with L. casei or E. faecalis or a combination of L...
April 2017: Archives of Animal Nutrition
https://www.readbyqxmd.com/read/28198662/changes-in-bacterial-epidemiology-and-antibiotic-resistance-among-veterans-with-spinal-cord-injury-disorder-over-the-past-9-years
#19
Margaret A Fitzpatrick, Katie J Suda, Nasia Safdar, Stephen P Burns, Makoto M Jones, Linda Poggensee, Swetha Ramanathan, Charlesnika T Evans
OBJECTIVE: Patients with spinal cord injury and disorder (SCI/D) have an increased risk of infection with multidrug-resistant (MDR) bacteria. We described bacterial epidemiology and resistance in patients with SCI/D at Veterans Affairs Medical Centers (VAMCs) for the past 9 years. DESIGN: Retrospective cohort Setting: One hundred thirty VAMCs. PARTICIPANTS: Veterans with SCI/D and bacterial cultures with antibiotic susceptibility testing performed between 1/1/2005-12/31/2013...
February 15, 2017: Journal of Spinal Cord Medicine
https://www.readbyqxmd.com/read/28198553/treatment-of-septic-arthritis-of-the-coxofemoral-joint-in-12-foals
#20
F Barceló Oliver, T M Russell, K L Uprichard, K M Neil, P J Pollock
OBJECTIVE: To describe the clinical signs, surgical treatment, and outcome of septic arthritis of the coxofemoral joint in foals. STUDY DESIGN: Retrospective clinical study. SAMPLE POPULATION: Foals (n = 12) with confirmed sepsis of the coxofemoral joint. METHODS: Lameness was localized to the coxofemoral joint based on physical examination. Sepsis was confirmed by cytological analysis of synovial fluid obtained under ultrasonographic guidance, during general anesthesia or standing sedation...
February 15, 2017: Veterinary Surgery: VS
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