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https://www.readbyqxmd.com/read/28453512/a-reproducible-semi-automatic-method-to-quantify-the-muscle-lipid-distribution-in-clinical-3d-ct-images-of-the-thigh
#1
Alexander Mühlberg, Oleg Museyko, Jean-Denis Laredo, Klaus Engelke
Many studies use threshold-based techniques to assess in vivo the muscle, bone and adipose tissue distribution of the legs using computed tomography (CT) imaging. More advanced techniques divide the legs into subcutaneous adipose tissue (SAT), anatomical muscle (muscle tissue and adipocytes within the muscle border) and intra- and perimuscular adipose tissue. In addition, a so-called muscle density directly derived from the CT-values is often measured. We introduce a new integrated approach to quantify the muscle-lipid system (MLS) using quantitative CT in patients with sarcopenia or osteoporosis...
2017: PloS One
https://www.readbyqxmd.com/read/28448004/live-confocal-imaging-of-developing-arabidopsis-flowers
#2
Nathanaël Prunet
The study of plant growth and development has long relied on experimental techniques using dead, fixed tissues and lacking proper cellular resolution. Recent advances in confocal microscopy, combined with the development of numerous fluorophores, have overcome these issues and opened the possibility to study the expression of several genes simultaneously, with a good cellular resolution, in live samples. Live confocal imaging provides plant biologists with a powerful tool to study development, and has been extensively used to study root growth and the formation of lateral organs on the flanks of the shoot apical meristem...
April 1, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28446997/endoscopic-submucosal-dissection-and-endoscopic-mucosal-resection-for-early-stage-esophageal-cancer
#3
REVIEW
Bo Ning, Mohamed M Abdelfatah, Mohamed O Othman
Mortality from esophageal cancer remains high despite advances in medical therapy. Although the incidence of squamous cell carcinoma of the esophagus remains unchanged, the incidence of the esophageal adenocarcinoma has increased over time. Gastroesophageal reflux disease (GERD and obesity are contributing factors to the development of Barrett's esophagus and subsequent development of adenocarcinoma. Early recognition of the disease can lead to resection of esophageal cancer prior to the development of lymphovascular invasion...
March 2017: Annals of Cardiothoracic Surgery
https://www.readbyqxmd.com/read/28445365/improvement-of-flap-necrosis-in-a-rat-random-skin-flap-model-by-in-vivo-electroporation-mediated-hgf-gene-transfer
#4
S Morteza Seyed Jafari, Maziar Shafighi, Helmut Beltraminelli, Thomas Geiser, Robert E Hunger, Amiq Gazdhar
BACKGROUND: Despite great understanding of underlying mechanisms for flap necrosis and advances in surgical techniques, flap necrosis remains a critical issue. In the present study, the authors investigated the efficacy of electroporation-mediated hepatocyte growth factor (HGF) gene delivery to random dorsal skin flaps (McFarlane) to accelerate wound healing and reduce flap necrosis. METHODS: Fifteen male Wistar rats (290 to 320 g) were divided randomly into three groups...
May 2017: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/28445243/harmonizing-standardized-uptake-value-recovery-between-two-pet-ct-systems-from-different-manufacturers-when-using-resolution-modelling-and-time-of-flight
#5
Ian S Armstrong, Katharine E Thomson, Lisa M Rowley, Daniel R McGowan
PET iterative reconstruction algorithms with resolution modelling (RM) can be used to improve spatial resolution in the images. However, RM has a significant impact on quantification, which raises issues for harmonization across multicentre networks or collaborations. This investigation compared quantification from two modern time-of-flight (TOF) PET/CT systems from different manufacturers with RM with the intention to harmonize recovery. Images of a National Electrical Manufacturers Association image quality phantom with a sphere-to-background concentration ratio of 4 : 1 were acquired on a GE Discovery 710 and a Siemens Biograph mCT and reconstructed with RM and TOF...
April 25, 2017: Nuclear Medicine Communications
https://www.readbyqxmd.com/read/28444180/left-bundle-branch-block-from-cardiac-mechanics-to-clinical-and-diagnostic-challenges
#6
Elena Surkova, Luigi P Badano, Roberto Bellu, Patrizia Aruta, Federica Sambugaro, Gabriella Romeo, Federico Migliore, Denisa Muraru
Left bundle branch block (LBBB) results in an altered pattern of left ventricular (LV) activation and subsequent contraction, causing remarkable changes in LV mechanics, perfusion and workload and ultimately leading to pathologic cardiac remodelling. Clinical and diagnostic notions about the LBBB phenomenon had evolved from just an electrocardiographic pattern to a critically important finding affecting diagnostic and clinical management of many patients and adversely influencing their outcomes. Recent advances in imaging techniques significantly improved the assessment of patients with LBBB and provided additional insights into pathophysiological mechanisms of LV remodelling...
April 20, 2017: Europace: European Pacing, Arrhythmias, and Cardiac Electrophysiology
https://www.readbyqxmd.com/read/28442858/observer-variability-in-evaluating-pisotriquetral-osteoarthritis-using-pisotriquetral-view
#7
Erik Heeg, Paul W L Ten Berg, Mario Maas, Simon D Strackee
A pisotriquetral (semilateral) view of the wrist may improve the assessment of pisotriquetral osteoarthritis (OA), but its reliability and reproducibility are unclear. The purpose of this cross-sectional observer study was to investigate (1) the inter- and intraobserver agreement of evaluating pisotriquetral OA using pisotriquetral views with a special focus on sclerosis, joint space width (JSW) narrowing and osteophyte formation, and (2) the incidence of these latter radiographic features in patients suspected for pisotriquetral OA...
April 2017: Journal of Hand and Microsurgery
https://www.readbyqxmd.com/read/28442330/symptomatic-carotid-artery-disease-revascularization
#8
REVIEW
Piero Montorsi, Stefano Galli, Paolo M Ravagnani, Marco Roffi
Patients with symptomatic carotid artery disease should be managed by a multidisciplinary team including neurologists, vascular surgeons and interventionalists. Duplex ultrasound is the most widely used diagnostic modality to assess carotid disease, followed by additional imaging tests (CT- or MR-angiography) to confirm the severity of the stenosis, detect brain lesions, and assess intracranial circulation as well as the supra-aortic anatomy. Although overall randomized trial results favored (CEA) over carotid artery stenting (CAS) in symptomatic patients, this was likely related to the insufficient expertise of the endovascular specialists in several of the trials...
April 22, 2017: Progress in Cardiovascular Diseases
https://www.readbyqxmd.com/read/28441641/low-volume-contrast-ct-angiography-via-pulmonary-artery-injection-for-measurement-of-aortic-annulus-in-patients-undergoing-transcatheter-aortic-valve-replacement
#9
Vien T Truong, Joseph Choo, Luke McCoy, Adam Mussman, Stephanie Ambach, Dean Kereiakes, Ian Sarembock, Wojciech Mazur
OBJECTIVES: To investigate the feasibility and image quality of low-dose contrast computed tomography (CT) angiography with pulmonary artery (PA) protocol. BACKGROUND: Aortic stenosis is the most common valvular heart disease and transcatheter aortic valve replacement (TAVR) has evolved as an alternative method for surgical valve replacement in intermediate-risk and high-risk surgical patients. CT is essential for measurement of aortic annulus prior to TAVR. METHODS: Twenty patients underwent a low-dose contrast study with PA protocol and 20 patients underwent a traditional-dose study (traditional protocol)...
May 2017: Journal of Invasive Cardiology
https://www.readbyqxmd.com/read/28441369/cardiac-magnetic-resonance-imaging-in-oncology
#10
Daniel Jeong, Aarti Patel, Christopher J Francois, Kenneth L Gage, Michael G Fradley
BACKGROUND: Cardiac magnetic resonance imaging (MRI) is emerging as an important diagnostic modality in the management of cardiovascular-related dysfunction in oncological diseases. Advances in imaging techniques have enhanced the detection and evaluation of cardiac masses; meanwhile, innovative applications have created a growing role for cardiac MRI for the management of cardiotoxicity caused by cancer therapies. METHODS: An overview is provided of the clinical indications and technical considerations of cardiac MRI...
April 2017: Cancer Control: Journal of the Moffitt Cancer Center
https://www.readbyqxmd.com/read/28441368/skeletal-scintigraphy
#11
Jaime L Montilla-Soler, Rikesh Makanji
BACKGROUND: Skeletal scintigraphy remains a valuable tool in the initial and subsequent evaluation of the skeletal system in patients with a diagnosis of primary or metastatic neoplasms. METHODS: We discuss radiopharmaceuticals, nuclear medicine imaging techniques, and current as well as future oncological applications in the adult population. Pertinent literature was reviewed to describe the advantages and limitations of available technologies for the evaluation of skeletal metastatic disease...
April 2017: Cancer Control: Journal of the Moffitt Cancer Center
https://www.readbyqxmd.com/read/28440620/water-proton-nmr-a-tool-for-protein-aggregation-characterization
#12
Marc B Taraban, Roberto A DePaz, Brian Lobo, Yihua Bruce Yu
Formulation stability is a critical attribute of any protein-based biopharmaceutical drug due to a protein's inherent tendency to aggregate. Advanced analytical techniques currently used for characterization of protein aggregates are prone to a number of limitations, and usually require additional manipulations with the sample, such as dilution, separation, labeling and use of special cuvettes. In the present work, we compared conventional techniques for the analysis of protein aggregates with a novel approach that employs the water proton transverse relaxation rate R2(1H2O)...
April 25, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28440246/what-s-new-for-us-in-strabismus
#13
Pradeep Sharma, Nripen Gaur, Swati Phuljhele, Rohit Saxena
Strabismus is one of the most challenging subspecialties encountered in the field of ophthalmology. The concept of etiology of strabismus is being advanced with the development of newer imaging modalities and increased understanding of the genetics of strabismus. Imaging is also being used to aid in the planning of strabismus surgery. Newer horizons are being explored in the amblyopia management. The good old eye-pad is being replaced with the iPad. Early detection of loss of stereopsis is being used to decide the timing for strabismus surgery...
March 2017: Indian Journal of Ophthalmology
https://www.readbyqxmd.com/read/28439869/studying-protein-protein-interactions-in-planta-using-advanced-fluorescence-microscopy
#14
Marc Somssich, Rüdiger Simon
The formation of protein complexes through direct protein-protein interaction is essential for virtually every biological process, and accordingly the ability to determine the interaction properties of specific proteins is important to understand these processes. Förster resonance energy transfer (FRET) measurements are state-of-the-art confocal fluorescence microscopy- and imaging-based techniques that allow the analysis of protein interactions in vivo and in planta, in specific compartments of single cells or tissues...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28438675/integrating-cell-biology-and-proteomic-approaches-in-plants
#15
Tomáš Takáč, Olga Šamajová, Jozef Šamaj
Significant improvements of protein extraction, separation, mass spectrometry and bioinformatics nurtured advancements of proteomics during the past years. The usefulness of proteomics in the investigation of biological problems can be enhanced by integration with other experimental methods from cell biology, genetics, biochemistry, pharmacology, molecular biology and other omics approaches including transcriptomics and metabolomics. This review aims to summarize current trends integrating cell biology and proteomics in plant science...
April 21, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28438082/radioimmunoconjugates-for-treating-cancer-recent-advances-and-current-opportunities
#16
Mickaël Bourgeois, Clément Bailly, Mathieu Frindel, François Guerard, Michel Chérel, Alain Faivre-Chauvet, Françoise Kraeber-Bodéré, Caroline Bodet-Milin
Radioimmunoconjugates have been used for 30 years to diagnose and treat cancer. For many years, the use of these therapeutic tools has been limited to haematological disorders, such as non-Hodgkin's lymphoma, given that they have only had a moderate effect on solid tumours. Areas covered: Recently, several strategies have revived the potential therapeutic application for radioimmunoconjugates. In this review, the authors review the advances in immunological engineering to develop new tools like monoclonal antibodies and their derivatives...
April 24, 2017: Expert Opinion on Biological Therapy
https://www.readbyqxmd.com/read/28438024/biomedical-surface-analysis-evolution-and-future-directions-review
#17
David G Castner
This review describes some of the major advances made in biomedical surface analysis over the past 30-40 years. Starting from a single technique analysis of homogeneous surfaces, it has been developed into a complementary, multitechnique approach for obtaining detailed, comprehensive information about a wide range of surfaces and interfaces of interest to the biomedical community. Significant advances have been made in each surface analysis technique, as well as how the techniques are combined to provide detailed information about biological surfaces and interfaces...
April 24, 2017: Biointerphases
https://www.readbyqxmd.com/read/28437571/label-free-high-temporal-resolution-assessment-of-cell-proliferation-using-digital-holographic-microscopy
#18
Birgit Janicke, Andreas Kårsnäs, Peter Egelberg, Kersti Alm
Cell proliferation assays are widely applied in biological sciences to understand the effect of drugs over time. However, current methods often assess cell population growth indirectly, that is, the cells are not actually counted. Instead other parameters, for example, the amount of protein, are determined. These methods often also demand phototoxic labels, have low temporal resolution, or employ end-point assays, and frequently are labor intensive. We have developed a robust and label-free kinetic cell proliferation assay with high temporal resolution for adherent cells using digital holographic microscopy (DHM), one of many quantitative phase microscopy techniques...
April 24, 2017: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/28437493/the-role-of-medial-patellofemoral-ligament-repair-and-imbrication
#19
Kyle R Duchman, Matthew J Bollier
Repair, reefing, and advancement of the medial patellofemoral ligament (MPFL) and medial retinacular structures can be performed as an isolated procedure or in conjunction with distal realignment procedures for patients with patellar instability. Although various operative techniques have been described, understanding the appropriate clinical indications and MPFL injury patterns ultimately determines the success or failure of the procedure. MPFL repair is best indicated in the acute setting, particularly if there is a patella- or femoral-based avulsion...
March 2017: American Journal of Orthopedics
https://www.readbyqxmd.com/read/28436854/manifold-embedding-and-semantic-segmentation-for-intraoperative-guidance-with-hyperspectral-brain-imaging
#20
Daniele Ravi, Himar Fabelo, Gustavo Marrero Callico, GuangZhong Yang
Recent advances in hyperspectral imaging have made it a promising solution for intra-operative tissue characterization, with the advantages of being non-contact, non-ionizing and non-invasive. Working with hyperspectral images in vivo, however, is not straightforward as the high dimensionality of the data makes real-time processing challenging. In this paper, a novel dimensionality reduction scheme and a new processing pipeline are introduced to obtain a detailed tumour classification map for intra-operative margin definition during brain surgery...
April 24, 2017: IEEE Transactions on Medical Imaging
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