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Yuelu Zhang, Liang Wang, Yuechan Zhang, Mo Wang, Qinglei Sun, Fangzhou Xia, Ruobing Wang, Lin Liu
BACKGROUND/AIMS: Nogo-B, a conservative protein of endoplasmic reticulum, is a member of the reticulon family of proteins. Proliferative diabetic retinopathy (PDR) is the major concerning problem of diabetic retinopathy. This study explored the role of Nogo-B in the regulation of angiogenesis in PDR patients and primary human retinal endothelial cells (HRMECs). METHODS: Nogo-B was down-regulated through the use of Lentivirus-NogoB-RNAi, the effects of Nogo-B on angiogenesis under high glucose stimulation were evaluated via CCK-8 assay, wound closure assay, transwell assay, and tube formation assay...
October 19, 2017: Cellular Physiology and Biochemistry
Seok Hee Lee, Hyun Ju Oh, Min Jung Kim, Geon A Kim, Yoo Bin Choi, Young Kwang Jo, Erif Maha Nugraha Setyawan, Byeong Chun Lee
In the estrus stage, canine oocytes are surrounded by cumulus cells and undergo maturation in the oviduct for 2-3 days after ovulation. We hypothesized that canine oviduct cells (cOC) and canine cumulus cells (cCC) during this stage might affect the maturation of oocytes and thereby improve subsequent embryo development. Therefore, the objective of this study was to compare the effects of a cOC and cCC co-culture on oocyte in vitro maturation (IVM) and subsequent embryo development, and to analyze the gene expressions in a molecular fashion what co-culture actually gives the specific pathways in which the co-culture cells act to improve maturation and embryo development...
October 3, 2017: Theriogenology
Ya Li, Yaguang Weng, Liang Zhong, Huimin Chong, Sicheng Chen, Yanting Sun, Wang Li, Qiong Shi
In lung adenocarcinoma, loss of p53 and PTEN in tumors are associated with decreased response to chemotherapy and decreased survival. A means to pharmacologically upregulate p53 and PTEN protein expression could improve the prognosis of patients with p53- and PTEN-deficient tumors. In the present study we revealed that vascular endothelial growth factor receptor 3 (VEGFR3) inhibition in lung adenocarcinoma cells was associated with improved expression levels of both p53 and PTEN in the tumor-associated macrophage (TAM) microenvironment...
September 19, 2017: Oncology Reports
Tae Hee Kim, Youngmee Jung, Soo Hyun Kim
Produced via electrospinning, poly(l-lactide-co-caprolactone) (PLCL) membranes, which have a porous structure and are biodegradable, are of interest in various medical fields. The porous-structured electrospun membrane is particularly interesting because of several favorable properties: it exudes fluid from the wound, does not build up under the wound covering, and does not cause wound desiccation. Moreover, extracellular matrix (ECM)-based structures derived by tissue decellularization have application as engineered tissue scaffolds and as supports for cellular regeneration...
October 19, 2017: Tissue Engineering. Part A
Feng-Ling Liao, Lin Tan, Hua Liu, Jin-Ju Wang, Xiao-Tang Ma, Bin Zhao, Yan-Fang Chen, Ji Bihl, Yi Yang, Ri-Ling Chen
Cell-derived exosomes (EXs) can modulate target cell differentiation via microRNAs (miRs) that they carried. Previous studies have shown that miR126 is highly expressed in hematopoietic stem cells (HSCs) and plays a role in hematopoiesis via modulating the Notch pathway that participates in progenitors' cell fate decisions. In this study we investigated whether HSC-derived EXs (HSC-EXs) could affect the differentiation of mouse embryonic stem cells (ESCs) into HSCs. We prepared HSC-EXs(con), HSC-EXs(sc) and HSC-EXs(miR126) from control HSCs and the HSCs transfected with scramble control or miR126 mimics, respectively...
October 19, 2017: Acta Pharmacologica Sinica
Jordan A Anderson, Sujan Lamichhane, Thomas Vierhout, Daniel Engebretson
Smooth muscle cells (SMCs) and macrophages are important cellular components involved in the development of complications following the implantation of cardiovascular devices. This leads to various disorders such as restenosis, chronic inflammation, and may ultimately result in device failure. In this study, we developed a post-implant stent co-culture model using different ratios of SMCs and macrophages seeded on to cobalt-chromium alloy. The macrophages had an increased affinity to the co-culture surfaces, which resulted in decreased SMC attachment to the alloy surfaces at the initial time point...
October 19, 2017: Journal of Biomedical Materials Research. Part A
R L Yang, T T Yu, Y H Zhou
OBJECTIVE: To analyze the role of acetylsalicylic acid (ASA) in immunomodulation of mesenchymal stem cells derived from gingiva (GMSCs), and to explore the role of ASA in enhancing the immumomodulation of GMSCs and the capacity of GMSCs to treat immune disorders and the underlying mechanism. METHODS: Flow cytometry analysis were used to analyze the role of ASA in the expression of stem cells surface markers CD146, CD105, CD90, CD34 and CD45 in GMSCs,and the GMSCs proliferation was analyzed by 5-bromo-2-deoxyuridine (BrdU) staining and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay...
October 18, 2017: Beijing da Xue Xue Bao. Yi Xue Ban, Journal of Peking University. Health Sciences
W Chen, F L Hu, H J Liu, L L Xu, Y N Li, Z G Li
OBJECTIVE: To investigate the effect of myeloid-derived suppressor cells (MDSC) on pro-liferation of B lymphocytes in rheumatoid arthritis (RA) patients. METHODS: The peripheral blood specimens were collected from 15 healthy adults and 38 RA patients who were divided into high disease activity group, medium activity group and low activity group according to their 28-joint disease activity score (DAS28). And the frequencies of MDSC were determined by flow cytometry...
October 18, 2017: Beijing da Xue Xue Bao. Yi Xue Ban, Journal of Peking University. Health Sciences
Jiheum Park, Gi-Hun Lee, Joong Yull Park, Jung Chan Lee, Hee Chan Kim
In living tissue, cells exist in three-dimensional (3D) microenvironments with intricate cell-cell interactions. To model these cellular environments, numerous techniques for generating cell spheroids have been proposed and improved. However, previously reported methods still have limitations in uniformity, reproducibility, scalability, through-put, etc. Here, we present a centrifugal microfluidic-based spheroid (CMS) formation method for generating both co-culture and mono-culture 3D spheroids in a highly controlled manner...
October 18, 2017: Biofabrication
Phoompong Boonsaen, Madoka Kinjo, Suriya Sawanon, Yutaka Suzuki, Satoshi Koike, Yasuo Kobayashi
The fibrolytic rumen bacterium Ruminococcus flavefaciensOS14 was isolated from swamp buffalo and its phylogenetic, ecological and digestive properties were partially characterized. Isolates from rumen contents of four swamp buffalo were screened for fibrolytic bacteria; one of the 40 isolates showed a distinctive feature of solubilizing cellulose powder in liquid culture and was identified as R. flavefaciens based on its 16S ribosomal DNA sequence. This isolate, OS14, was employed for detection and digestion studies, for which a quantitative PCR assay was developed and defined cultures were tested with representative forages in Thailand...
October 17, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
J O Rushton, E Kammergruber, A Tichy, M Egerbacher, B Nell, S Gabner
BACKGROUND: Despite advances in therapy of corneal ulcerative diseases in horses, a vast number of cases require surgical intervention, due to poor response to treatment. Topical application of serum has been used for many years, based on its anti-collagenolytic properties and the presence of growth factors promoting corneal wound healing. However, although other blood derived products i.e. platelet rich plasma (PRP), plasma rich in growth factors (PRGF) have been widely used in equine orthopaedics and in human ophthalmology, no reports of the effects of these blood derived products exist in equine ophthalmology...
October 16, 2017: Equine Veterinary Journal
Paul E Pfeffer, Tzer R Ho, Elizabeth H Mann, Frank J Kelly, Maria Sehlstedt, Jamshid Pourazar, Rosamund E Dove, Thomas Sandstrom, Ian S Mudway, Catherine M Hawrylowicz
Epidemiological studies have consistently shown associations between elevated concentrations of urban particulate matter (UPM) air pollution and exacerbations of asthma and COPD, which are both associated with viral respiratory infections. The effects of urban PM on dendritic cell (DC) stimulated CD4 T lymphocytes have been investigated previously, however little work has focused on CD8 T lymphocyte responses despite their importance in anti-viral immunity. To address this, we examined the effects of UPM on DC-stimulated naïve CD8 T cell responses...
October 16, 2017: Immunology
Haiyan Hu, Huijuan Li, Yuanli He
The aim of the current study was to evaluate the expression of microRNA (miR)-17 in the endometrial tissues of patients with adenomyosis (AM) and determine its biological function in the occurrence and development of the disease. A total of 45 fresh endometrial tissues of AM patients and 32 normal endometrial tissues were collected from healthy controls. The expression of miR-17 was evaluated using reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The miR-17-targeting gene phosphatase and tensin homolog (PTEN) was predicted using bioinformatics and its expression was evaluated with RT-qPCR and western blot analysis...
October 2017: Experimental and Therapeutic Medicine
Zhou Xin Yang, Ying Chi, Yue Ru Ji, You Wei Wang, Jing Zhang, Wei Feng Luo, Li Na Li, Cai Dong Hu, Guang Sheng Zhuo, Li Fang Wang, Zhi-Bo Han, Zhong Chao Han
Mesenchymal stem cells (MSC) are able to differentiate into cells of multiple lineage, and additionally act to modulate the immune response. Interleukin (IL)-9 is primarily produced by cluster of differentiation (CD)4(+) T cells to regulate the immune response. The present study aimed to investigate the effect of human umbilical cord derived-MSC (UC-MSC) on IL-9 production of human CD4(+) T cells. It was demonstrated that the addition of UC-MSC to the culture of CD4(+) T cells significantly enhanced IL-9 production by CD4(+) T cells...
October 2017: Experimental and Therapeutic Medicine
Lin Shen, Peng Sun, Canwei Zhang, Le Yang, Liqun Du, Xinyi Wu
Corneal endothelial dysfunction occurs when corneal endothelial cells (CECs) are dramatically lost and eventually results in vision loss. Corneal transplantation is the only solution at present. However, corneal transplantation requires a fresh human cornea and there is a worldwide shortage of donors. Therefore, finding new functional CECs to replace human CECs is urgent. Skin-derived precursors (SKPs) can be easily acquired and have multiple differential potential. We co-cultured human SKPs with B4G12 cells in serum-free medium and obtained abundant CEC-like cells which had similar morphology and characteristic to human CECs...
October 17, 2017: Scientific Reports
Koji Sakai, Kenta Shimba, Kazuma Ishizuka, Zhuonan Yang, Kosuke Oiwa, Akimasa Takeuchi, Kiyoshi Kotani, Yasuhiko Jimbo
Microelectrode array (MEA) based-drug screening with human induced pluripotent stem cell-derived cardiomyocytes (hiPSCM) is a potent pre-clinical assay for efficiently assessing proarrhythmic risks in new candidates. Furthermore, predicting sympathetic modulation of the proarrhythmic side-effects is an important issue. Although we have previously developed a MEA-based co-culture system of rat primary cardiomyocyte and sympathetic neurons (rSNs), it is unclear if this co-culture approach is applicable to develop and investigate sympathetic innervation of hiPSCMs...
October 14, 2017: Biochemical and Biophysical Research Communications
Christina Nürnberg, Norbert Kociok, Claudia Brockmann, Timo Lischke, Sergio Crespo-Garcia, Nadine Reichhart, Susanne Wolf, Ria Baumgrass, Sabine A Eming, Sandra Beer-Hammer, Antonia M Joussen
Anti-VEGF-directed therapies have been a milestone for treating retinal vascular diseases. Depletion of monocyte lineage cells suppresses pathological neovascularization in the oxygen-induced retinopathy mouse model. However, the question whether myeloid-derived VEGF-A expression is responsible for the pathogenesis in oxygen-induced retinopathy remained unknown. We analyzed LysMCre-driven myeloid cell-specific VEGF-A knockout mice as well as mice with complete depletion of circulating macrophages through clodronate-liposome treatment in the oxygen-induced retinopathy model by immunohistochemistry, qPCR, and flow cytometry...
October 14, 2017: Experimental Eye Research
Lotta Parviainen, Sybille Dihanich, Greg W Anderson, Andrew M Wong, Helen R Brooks, Rosella Abeti, Payam Rezaie, Giovanna Lalli, Simon Pope, Simon J Heales, Hannah M Mitchison, Brenda P Williams, Jonathan D Cooper
The neuronal ceroid lipofuscinoses (NCLs or Batten disease) are a group of inherited, fatal neurodegenerative disorders of childhood. In these disorders, glial (microglial and astrocyte) activation typically occurs early in disease progression and predicts where neuron loss subsequently occurs. We have found that in the most common juvenile form of NCL (CLN3 disease or JNCL) this glial response is less pronounced in both mouse models and human autopsy material, with the morphological transformation of both astrocytes and microglia severely attenuated or delayed...
October 17, 2017: Acta Neuropathologica Communications
Qun Wu, Jianchun Lin, Kaixiang Cui, Yang Zhu, Yan Xu
Urea is an important intermediate to synthesize carcinogenic ethyl carbamate in various foods fermentations. Identifying urea-producing microorganisms can help control or reduce ethyl carbamate production. Using Chinese liquor fermentation as a model system, we identified yeasts responsible for urea production. Urea production was positively correlated to yeasts population (R = 0.523, P = 0.045). Using high-throughput sequencing, we identified 26 yeast species. Partial least square regression and correlation analysis indicated that Wickerhamomyces anomalus was the most important yeast to produce urea (variable important plot = 1...
October 9, 2017: FEMS Yeast Research
Subrata K Pore, Eun-Ryeong Hahm, Joseph D Latoche, Carolyn J Anderson, Yongli Shuai, Shivendra V Singh
Osteolytic bone resorption is the primary cause of pain and suffering (e.g., pathological bone fracture) in women with metastatic breast cancer. The current standard of care for patients with bone metastasis for reducing the incidence of skeletal complications includes bisphosphonates and a humanized antibody (denosumab). However, a subset of patients on these therapies still develops new bone metastasis or experience adverse effects. Moreover, some bisphosphonates have poor oral bioavailability. Therefore, orally-bioavailable and non-toxic inhibitors of breast cancer-induced osteolytic bone resorption are still clinically desirable...
October 11, 2017: Carcinogenesis
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