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https://www.readbyqxmd.com/read/29458119/the-function-of-hla-b-13-01-involved-in-the-pathomechanism-of-dapsone-induced-severe-cutaneous-adverse-reactions
#1
Wei-Ti Chen, Chuang-Wei Wang, Chun-Wei Lu, Chun-Bing Chen, Hua-En Lee, Shuen-Iu Hung, Siew-Eng Choon, Chih-Hsun Yang, Ming-Tsan Liu, Ting Jui Chen, Wen-Lang Fan, Shih-Chi Su, Yang Yu-Wei Lin, Ya-Ching Chang, Wen-Hung Chung
Dapsone-induced hypersensitivity reactions may cause severe cutaneous adverse reactions (SCAR), such as drug reaction with eosinophilia and systemic symptoms (DRESS). It has been reported that HLA-B*13:01 is strongly associated with dapsone-induced hypersensitivity reactions among leprosy patients. However, the phenotype specificity and detailed immune mechanism of HLA-B*13:01 remains unclear. We investigated the genetic predisposition, HLA-B*13:01 function, and cytotoxic T cells (CTLs) involved in pathogenesis of dapsone-SCAR...
February 16, 2018: Journal of Investigative Dermatology
https://www.readbyqxmd.com/read/29457853/mesenchymal-epithelial-transition-of-pancreatic-cancer-cells-at-perineural-invasion-sites-is-induced-by-schwann-cells
#2
Yoko Fujii-Nishimura, Ken Yamazaki, Yohei Masugi, Junya Douguchi, Yutaka Kurebayashi, Naoto Kubota, Hidenori Ojima, Minoru Kitago, Masahiro Shinoda, Akinori Hashiguchi, Michiie Sakamoto
Epithelial-mesenchymal transition (EMT) promotes invasion and metastasis of pancreatic ductal adenocarcinoma (PDAC). However, the importance of its reverse process, mesenchymal-epithelial transition (MET), for PDAC remains unclear. We aimed to characterize the histological finding "focal differentiation" in PDAC at perineural invasion sites in the context of MET and to investigate the role of Schwann cells in inducing tumor MET. Tumor differentiation and immunohistochemical expressions of E-cadherin, SMAD3, and vimentin at perineural invasion sites were examined in 168 PDAC tissues...
February 19, 2018: Pathology International
https://www.readbyqxmd.com/read/29457648/reduction-of-infection-risk-meditated-by-co-culturing-vibrio-parahaemolyticus-and-listeria-monocytogenes-in-refrigerated-cooked-shrimp
#3
Ben Niu, Lili Mu, Lili Xiao, Zhaohuan Zhang, Pradeep K Malakar, Haiquan Liu, Yingjie Pan, Yong Zhao
BACKGRROUD: Vibrio parahaemolyticus and Listeria monocytogenes are seafood pathogens of public health significance and predictive models are effective tools for quantitative microbial risk assessment (QMRA) of these pathogens. However, most current predictive models are based on growth of single strains in broth cultures and interactions of two or more bacteria in a food matrix can skew the outcomes of the predictions. Therefore, the impact of V. parahaemolyticus and L. monocytogenes when co-cultured and in mono culture on cooked shrimp at cold storage was investigated in this study...
February 19, 2018: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/29456740/an-obligatory-anaerobic-salmonella-typhimurium-strain-redirects-m2-macrophages-to-the-m1-phenotype
#4
Mei Yang, Juan Xu, Qi Wang, An-Qin Zhang, Kun Wang
A genetically engineered Salmonella typhimurium strain that may be applied in the medically useful therapeutic strategy of using bacterial agents to target breast cancer in a tumor-bearing nude mouse model has been previously reported. Furthermore, immune cell accumulation in breast tumor types has been observed, particularly distributed in regions surrounding the bacteria. M2 macrophages are associated with breast cancer aggressiveness, whereas M1 macrophages are prone to devouring bacteria and killing cancer cells...
March 2018: Oncology Letters
https://www.readbyqxmd.com/read/29455681/human-dendritic-cell-sequestration-onto-the-necator-americanus-larval-sheath-during-ex-sheathing-a-possible-mechanism-for-immune-privilege
#5
A Hassan, D I Pritchard, A M Ghaemmaghami
Despite the profound health implications of Necator americanus infection in humans, many aspects of its interaction with the host immune system are poorly understood. Here we investigated the early events at the interface of N. americanus larvae (L3) and human dendritic cells (DCs). Our data show that co-culturing DCs and the larvae trigger ex-sheathing of hookworms rapidly where a majority of DCs are sequestered onto the larval sheath allowing the ex-sheathed larvae to migrate away unchallenged. Intriguingly, DCs show negligible interaction with the ex-sheathed larvae, alluding to differences between the surface chemistry of the larva and its sheath...
February 19, 2018: Parasitology
https://www.readbyqxmd.com/read/29455640/ccl2-ccl5-secreted-by-the-stroma-induce-il-6-pyk2-dependent-chemoresistance-in-ovarian-cancer
#6
Jennifer Pasquier, Marie Gosset, Caroline Geyl, Jessica Hoarau-Véchot, Audrey Chevrot, Marc Pocard, Massoud Mirshahi, Raphael Lis, Arash Rafii, Cyril Touboul
BACKGROUND: Minimal residual disease is the main issue of advanced ovarian cancer treatment. According to the literature and previous results, we hypothesized that Mesenchymal Stromal Cells (MSC) could support this minimal residual disease by protecting ovarian cancer cells (OCC) from chemotherapy. In vitro study confirmed that MSC could induce OCC chemoresistance without contact using transwell setting. Further experiments showed that this induced chemoresistance was dependent on IL-6 OCC stimulation...
February 19, 2018: Molecular Cancer
https://www.readbyqxmd.com/read/29455239/genotyping-of-candidatus-syngnamydia-salmonis-chlamydiales-simkaniaceae-co-cultured-in-paramoeba-perurans-amoebozoa-paramoebidae
#7
Are Nylund, Dario Pistone, Christiane Trösse, Steffen Blindheim, Linda Andersen, Heidrun Plarre
Candidatus Syngnamydia salmonis (Chlamydiales, Simkaniaceae) was described as an epitheliocystis-causing bacterium from the gills of Atlantic salmon (Salmo salar) in Norway. A bacterium showing 99.2% 16S rRNA identity to Cand. S. salmonis is able to multiply in Paramoeba perurans and based on the classification criteria this bacterium could represent the same species as Cand. S. salmonis. Sequencing the genome of the cultured bacterium has made it possible to fulfill the minimal standards for genetic characterization of species within the order Chlamydiales...
February 17, 2018: Archives of Microbiology
https://www.readbyqxmd.com/read/29455109/dysregulation-of-jak-stat-genes-by-vasoactive-intestinal-peptide-vip-in-salmonella-infected-monocytes-may-inhibit-its-therapeutic-potential-in-human-sepsis
#8
Hiba Ibrahim, Basim Askar, Paul Barrow, Neil Foster
Murine/LPS models of Gram negative sepsis indicate that vasoactive intestinal peptide (VIP) has therapeutic potential. We investigated the unknown effect of VIP on JAK/STAT proteins and genes in human monocytes infected with Salmonella Typhimurium 14028. S. Typhimurium 14028 increased expression of both IL-6 receptor (IL-6R) and interferon gamma receptor 1 (IFNγR1) on monocytes but co-culture of infected monocytes with VIP (10-7 M) only decreased expression of IFNγR1 (P < 0.05). In contrast, S. Typhimurium 14028 infection or co-culture with VIP had no effect on IL-10 receptor expression on the monocyte surface...
February 15, 2018: Cytokine
https://www.readbyqxmd.com/read/29455095/establishment-of-pancreatic-microenvironment-model-of-er-stress-quercetin-attenuates-%C3%AE-cell-apoptosis-by-invoking-nitric-oxide-cgmp-signaling-in-endothelial-cells
#9
Natarajan Suganya, Krishna Priya Mani, Dornadula Sireesh, Palanisamy Rajaguru, Mariappanadar Vairamani, Thiruppathi Suresh, Takayoshi Suzuki, Suvro Chatterjee, Kunka Mohanram Ramkumar
The involvement of endoplasmic reticulum (ER) stress in endothelial dysfunction and diabetes-associated complications has been well documented. Inhibition of ER stress represents a promising therapeutic strategy to attenuate endothelial dysfunction in diabetes. Recent attention has focused on the development of small molecule inhibitors of ER stress to maintain endothelial homeostasis in diabetes. Here we have developed a reliable, robust co-culture system that allows a study on the endothelial cells and pancreatic β-cells crosstalk under ER stress and validated using a known ER stress modulator, quercetin...
January 3, 2018: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/29454997/regulatory-responses-of-hepatocytes-macrophages-and-vascular-endothelial-cells-to-magnesium-deficiency
#10
Mei Shigematsu, Shozo Tomonaga, Fumie Shimokawa, Masaru Murakami, Toru Imamura, Tohru Matsui, Masayuki Funaba
The liver is the organ that responds to nutritional disturbances including magnesium deficiency. The present study evaluated cellular responses to magnesium deficiency using model cells of the liver, namely, HepG2 cells as hepatocytes, RAW264.7 cells as Kupffer cells and human umbilical vein endothelial cells (HUVECs) as vascular endothelial cells; we examined effects of culture with magnesium deficient medium on cell responses in individual types of cells as well as interactive responses among cells. Metabolomic analyses indicated that magnesium deficiency differentially affected the cellular content of metabolites among HepG2 cells, RAW264...
February 2, 2018: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/29454805/mouse-preantral-follicle-growth-in-3d-co-culture-system-using-human-menstrual-blood-mesenchymal-stem-cell
#11
Zahra Rajabi, Hossein Yazdekhasti, Seyed Mohammad Hossein Noori Mugahi, Mehdi Abbasi, Somaieh Kazemnejad, Abolfazl Shirazi, Masoumeh Majidi, Amir-Hassan Zarnani
Follicle culture provides a condition which can help investigators to evaluate various aspects of ovarian follicle growth and development and impact of different components and supplementations as well as presumably application of follicle culture approach in fertility preservation procedures. Mesenchymal Stem Cells (MSCs), particularly those isolated from menstrual blood has the potential to be used as a tool for improvement of fertility. In the current study, a 3D co-culture system with mice preantral follicles and human Menstrual Blood Mesenchymal Stem Cells (MenSCs) using either collagen or alginate beads was designed to investigate whether this system allows better preantral follicles growth and development...
February 14, 2018: Reproductive Biology
https://www.readbyqxmd.com/read/29454023/interaction-between-hypothermia-and-delayed-mesenchymal-stem-cell-therapy-in-neonatal-hypoxic-ischemic-brain-injury
#12
Josephine Herz, Christian Köster, Barbara S Reinboth, Mark Dzietko, Wiebke Hansen, Hemmen Sabir, Cindy van Velthoven, Ivo Bendix, Ursula Felderhoff-Müser
Acute hypothermia treatment (HT) is the only clinically established intervention following neonatal hypoxic-ischemic brain injury. However, almost half of all cooled infants still die or suffer from long-lasting neurological impairments. Regenerative therapies, such as mesenchymal stem cells (MSC) appear promising as adjuvant therapy. In the present study, we hypothesized that HT combined with delayed MSC therapy results in augmented protection, improving long-term neurological outcome. Postnatal day 9 (P9) C57BL/6 mice were exposed to hypoxia-ischemia followed by 4 hours HT...
February 14, 2018: Brain, Behavior, and Immunity
https://www.readbyqxmd.com/read/29453928/cd4-cd25-cd127low-foxp3-regulatory-t-cells-in-crohn-s-disease
#13
Ali Khalili, Soheil Ebrahimpour, Iradj Maleki, Saeid Abediankenari, Mousa Mohammadnia Afrouzi
BACKGROUND: Regulatory T (Treg) cell play a key role in autoimmune diseases. We evaluated the regulatory function and frequency of Treg cells and secreted IL-10, IL-35 concentration in Crohn's disease (CD). METHODS: Twenty-three patients with CD and 25 healthy controls (HC) were included in this study. We analysed the alteration of Tregs frequency using flow cytometry for CD4, CD25, CD127 and FoxP3 markers. Surface expression of CD4, CD25 and CD127 markers were used for isolation of a relatively pure Treg cells...
February 16, 2018: Romanian Journal of Internal Medicine, Revue Roumaine de Médecine Interne
https://www.readbyqxmd.com/read/29453625/effects-of-feedstock-and-co-culture-of-lactobacillus-fermentum-and-wild-saccharomyces-cerevisiae-strain-during-fuel-ethanol-fermentation-by-the-industrial-yeast-strain-pe-2
#14
Vanda R Reis, Ana Paula G Bassi, Bianca C Cerri, Amanda R Almeida, Isis G B Carvalho, Reinaldo G Bastos, Sandra R Ceccato-Antonini
Even though contamination by bacteria and wild yeasts are frequently observed during fuel ethanol fermentation, our knowledge regarding the effects of both contaminants together is very limited, especially considering that the must composition can vary from exclusively sugarcane juice to a mixture of molasses and juice, affecting the microbial development. Here we studied the effects of the feedstock (sugarcane juice and molasses) and the co-culture of Lactobacillus fermentum and a wild Saccharomyces cerevisiae strain (rough colony and pseudohyphae) in single and multiple-batch fermentation trials with an industrial strain of S...
February 16, 2018: AMB Express
https://www.readbyqxmd.com/read/29453454/a-microvascularized-tumor-mimetic-platform-for-assessing-anti-cancer-drug-efficacy
#15
Shantanu Pradhan, Ashley M Smith, Charles J Garson, Iman Hassani, Wen J Seeto, Kapil Pant, Robert D Arnold, Balabhaskar Prabhakarpandian, Elizabeth A Lipke
Assessment of anti-cancer drug efficacy in in vitro three-dimensional (3D) bioengineered cancer models provides important contextual and relevant information towards pre-clinical translation of potential drug candidates. However, currently established models fail to sufficiently recapitulate complex tumor heterogeneity. Here we present a chip-based tumor-mimetic platform incorporating a 3D in vitro breast cancer model with a tumor-mimetic microvascular network, replicating the pathophysiological architecture of native vascularized breast tumors...
February 16, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29453199/simultaneous-assessment-of-clearance-metabolism-induction-and-drug-drug-interaction-potential-using-a-long-term-in-vitro-liver-model-for-a-novel-hepatitis-b-virus-inhibitor
#16
Nicole A Kratochwil, Miriam Triyatni, Martina B Mueller, Florian Klammers, Brian Leonard, Dan Turley, Josephine Schmaler, Aynur Ekiciler, Birgit Molitor, Isabelle Walter, Pierre-Alexis Gonsard, Charles A Tournillac, Alexandre Durrwell, Michaela Marschmann, Russell Jones, Mohammed Ullah, Franziska Boess, Giorgio Ottaviani, Yuyan Yin, Neil J Parrott, Stephen Fowler
Long-term in vitro liver models are now widely explored for human hepatic metabolic clearance prediction, enzyme phenotyping, cross species metabolism, comparison of low clearance drugs as well as induction studies. Here, we present studies using a long-term liver model which show how metabolism and active transport, drug-drug interactions and enzyme induction in healthy and diseased states, e.g. hepatitis B virus (HBV) infection, may be assessed in a single test system to enable effective data integration for PBPK modeling...
February 16, 2018: Journal of Pharmacology and Experimental Therapeutics
https://www.readbyqxmd.com/read/29453184/is-it-photosensitization-or-photodegradation-when-uv-b-irradiation-is-combined-with-bde-47-evidence-from-the-growth-and-reproduction-changes-of-rotifer-brachionus-plicatilis
#17
Chunchen Liu, Xuexi Tang, Bin Zhou, Yongshun Jiang, Mengchen Lv, Yu Zang, You Wang
Ecotoxicological methods were applied in the present study, and the marine rotifer Brachionus plicatilis was used as the toxic endpoint to depict what occurred when 2,2',4,4'-tetrabromodiphenyl ether (BDE-47) was combined with solar ultraviolet-B radiation (UV-B). B. plicatilis was exposed to three different combination methods of BDE-47 and UV-B at an equal toxicity ratio, including normal rotifer co-cultured with UV-B-irradiated BDE-47 (known as Method I), UV-B-irradiated rotifer co-cultured with BDE-47 exposure (known as Method II) and normal rotifer co-cultured with the simultaneous addition of BDE-47 and UV-B irradiation (known as Method III)...
February 13, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/29452534/co-culture-of-human-bone-marrow-mesenchymal-stem-cells-and-macrophages-attenuates-lipopolysaccharide-induced-inflammation-in-human-corneal-epithelial-cells
#18
Won-Yong Jeong, Ji-Hye Kim, Chan-Wha Kim
Dry eye syndrome (DES) is considered as an ocular surface inflammatory disease. Previous studies have shown inflammation plays an important role in the progression and onset of DES. Co-culture of human bone marrow mesenchymal stem cells (HBMSCs) and macrophages showed immunomodulatory effects via regulation of cytokine regulation. Thus, the aim of this study was to investigate the effect of the interaction of these cells on in vitro DES model. The conditioned media (CM) from macrophages, HBMSCs, and HBMSCs + macrophages were treated to human corneal epithelial cells, which showed significant reduction in IL-1α and IL-1β expression levels in HBMSCs + macrophages group...
February 16, 2018: Bioscience, Biotechnology, and Biochemistry
https://www.readbyqxmd.com/read/29452238/human-adipose-tissue-derived-stromal-cells-in-combination-with-exogenous-stimuli-facilitate-three-dimensional-network-formation-of-human-endothelial-cells-derived-from-various-sources
#19
Dominique Manikowski, Birgit Andrée, Esther Samper, Clémence Saint-Marc, Ruth Olmer, Peter Vogt, Sarah Strauß, Axel Haverich, Andres Hilfiker
In natural tissues, the nutrition of cells and removal of waste products is facilitated by a dense capillary network which is generated during development. This perfusion system is also indispensable for tissue formation in vitro. Nutrition depending solely on diffusion is not sufficient to generate tissues of clinically relevant dimensions, which is a core aim in tissue engineering research. In this study, the establishment of a vascular network was investigated in a self-assembling approach employing endothelial and mural cells...
February 13, 2018: Vascular Pharmacology
https://www.readbyqxmd.com/read/29451353/in-vitro-cytotoxicity-assessment-of-nanodiamond-particles-and-their-osteogenic-potential
#20
Mohamed Ibrahim, Ying Xue, Melanie Ostermann, Alexander Sauter, Doris Steinmueller-Nethl, Sarah Schweeberg, Anke Krueger, Mihaela R Cimpan, Kamal Mustafa
Scaffolds functionalized with nanodiamond particles (nDP) hold great promise with regard to bone tissue formation in animal models. Degradation of the scaffolds over time may leave nDP within the tissues, raising concerns about possible long-term unwanted effects. Human SaOS-2 osteoblast-like cells and U937 monoblastoid cells were exposed to 5 different concentrations (0.002-2 mg/L) of nDP (size range: 2.36 - 4.42 nm) for 24 h. Cell viability was assessed by impedance-based methods. The differential expression of stress and toxicity-related genes was evaluated by PCR super-array, while the expression of selected inflammatory and cell death markers was determined by qPCR...
February 16, 2018: Journal of Biomedical Materials Research. Part A
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