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https://www.readbyqxmd.com/read/29676778/indirect-co-cultures-of-healthy-mesenchymal-stem-cells-restore-the-physiological-phenotypic-profile-of-psoriatic-mesenchymal-stem-cells
#1
A Campanati, M Orciani, G Sorgentoni, V Consales, M Mattioli Belmonte, R Di Primio, A Offidani
Psoriasis microenvironment, characterized by an imbalance between Th1/Th17 and Th2 cytokines, influences also mesenchymal stem cells (MSCs) phenotypic profile. MSCs from healthy donors (H-MSCs) can exert a strong paracrine effect by secreting active soluble factors, able to modulate the inflammation in the microenvironment. To evaluate the influence of H-MSCs on MSCs from psoriatic patients (PsO-MSCs), H-MSCs and PsO-MSCs were isolated and characterized. Indirect co-culture of H-MSCs with PsO-MSCs was performed; effects on proliferation and expression of cytokines linked to Th1/Th17 and Th2 pathways were assayed before and after co-culture...
April 20, 2018: Clinical and Experimental Immunology
https://www.readbyqxmd.com/read/29676587/a-potential-role-for-exosomal-tctp-export-in-vascular-remodeling-in-pulmonary-arterial-hypertension
#2
Elisabet Ferrer, Benjamin J Dunmore, Dhiya Hassan, Mark L Ormiston, Stephen Moore, John Deighton, Lu Long, Xu Dong Yang, Duncan J Stewart, Nicholas W Morrell
Pulmonary arterial hypertension (PAH) is characterized by increased proliferation and resistance to apoptosis of pulmonary vascular cells. Increased expression of translationally controlled tumor protein (TCTP), a pro-survival and anti-apoptotic mediator, has recently been demonstrated in patients with hereditary PAH (HPAH) although its role in the pathobiology of PAH remains unclear. Silencing of TCTP in blood outgrowth endothelial cells (BOECs) isolated from control subjects led to significant changes in morphology, cytoskeletal organization, increased apoptosis and decreased directionality during migration...
April 20, 2018: American Journal of Respiratory Cell and Molecular Biology
https://www.readbyqxmd.com/read/29676133/-psoralen-inhibits-raw264-7-differentiation-into-osteoclasts-and-bone-resorption-by-regulating-cd4-t-cell-differentiation
#3
Jin-Ping Li, Bao-Ping Xie, Wen-Juan Zhang, Li-Ying Shi, Wei-Juan Li, Ying Zeng, Guo-Xing Gan, Yu-Hong Li
This paper aimed to investigate whether psoralen inhibits the differentiation and bone resorption by regulating CD4+T cell differentiation in RANKL-induced osteoclastogenesis in RAW264.7 cells, and elucidate its mechanism for osteoporosis. CD4+T cells were isolated from spleen cells of Balb/c mice by immunomagnetic separation method. The cells were divided into blank control group and psoralen group. The cells were cultured in 24-well plates and cultured for 3 days, and then they were collected for co-culture experiments after 4 days...
March 2018: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29675734/co-culture-system-of-hepatocytes-and-endothelial-cells-two-in-vitro-approaches-for-enhancing-liver-specific-functions-of-hepatocytes
#4
Gaoxiong Wang, Youshi Zheng, Yingchao Wang, Zhixiong Cai, Naishun Liao, Jingfeng Liu, Wenmin Zhang
Although hepatocyte transplantation and bioartificial liver support system provide new promising opportunities for those patients waiting for liver transplantation, hepatocytes are easily losing liver-specific functions by using the common in vitro cultured methods. The co-culture strategies with mimicking the in vivo microenvironment would facilitate the maintenance of liver-specific functions of hepatocytes. Considering that hepatocytes and endothelial cells (ECs) account for 80-90% of total cell populations in the liver, hepatocytes and ECs were directly co-cultured with hepatic stellate cells (HSCs) or adipose tissue-derived stem cells (ADSCs) at a ratio of 700:150:3 or 14:3:3 in the present study, and the liver-specific functions were carefully analyzed...
April 19, 2018: Cytotechnology
https://www.readbyqxmd.com/read/29675647/fabrication-of-agarose-concave-petridish-for-3d-culture-microarray-method-for-spheroids-formation-of-hepatic-cells
#5
Binbin Zhang, Yang Li, Gaoshang Wang, Zhidong Jia, Haiyan Li, Qing Peng, Yi Gao
Liver is one of the most important organ in the body. But there are many limitations about liver transplantation for liver failure. It is quite important to develop the xenogeneic biological liver for providing an alternation to transplantation or liver regeneration. In this paper, we proposed a method to construct a novel kind of agarose 3D-culture concave microwell array for spheroids formation of hepatic cells. Using the 3D printing method, the microwell array was fabricated with an overall size of 6.4 mm × 6...
April 19, 2018: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/29675543/improved-vaccine-induced-immune-responses-via-a-ros-triggered-nanoparticle-based-antigen-delivery-system
#6
Xiaoyu Liang, Jianwei Duan, Xuanling Li, Xiaowei Zhu, Youlu Chen, Xiaoli Wang, Hongfan Sun, Deling Kong, Chen Li, Jing Yang
Subunit vaccines that are designed based on recombinant antigens or peptides have shown promising potential as viable substitutes for traditional vaccines due to their better safety and specificity. However, the induction of adequate in vivo immune responses with appropriate effectiveness remains a major challenge for vaccine development. More recently, the implementation of a nanoparticle-based antigen delivery system has been considered a promising approach to improve the in vivo efficacy for subunit vaccine development...
April 20, 2018: Nanoscale
https://www.readbyqxmd.com/read/29674197/villous-explants-from-preeclamptic-placentas-induce-sflt1-in-pbmcs-an-ex-vivo-co-culture-study
#7
Stephen Kyle Gonzales, Martina Badell, Hanh Cottrell, Bassam Rimawi, Venkataraman Deepak, Neil Sidell, Augustine Rajakumar
OBJECTIVES: Soluble Flt1 (sFlt1) is an anti-angiogenic protein linked to the pathology of preeclampsia (PE). While the placenta serves as the major organ producing sFlt1 during normal pregnancy, peripheral blood mononuclear cells (PBMCs), endothelial cells, and stromal cells also produce sFlt1. The key question is 'what drives the overexpression of sFlt1 observed during PE?' In the present work we show evidence for sFlt1 over-expression in PBMCs due to interaction with placental villi from PE patients...
February 25, 2018: Pregnancy Hypertension
https://www.readbyqxmd.com/read/29673440/mir-93-5p-transferred-by-exosomes-promotes-the-proliferation-of-esophageal-cancer-cells-via-intercellular-communication-by-targeting-pten
#8
Meng Xin Liu, Juan Liao, Ming Xie, Zhi Kui Gao, Xiang Hu Wang, Ying Zhang, Mu He Shang, Li Hong Yin, Yue Pu Pu, Ran Liu
OBJECTIVE: To investigate the relationship between plasma miR-93-5p and the risk of esophageal cancer, as well as the influence of miR-93-5p on the biological function of esophageal cancer cells, exerted through exosomes. METHODS: The expression of plasma miR-93-5p in esophageal cancer patients and healthy controls was analysed by real-time quantitative PCR. The influence of miR-93-5p on the risk and prognosis of esophageal carcinoma was analyzed by conditional logistic regression and survival analysis...
March 2018: Biomedical and Environmental Sciences: BES
https://www.readbyqxmd.com/read/29673365/human-central-nervous-system-astrocytes-support-survival-and-activation-of-b-cells-implications-for-ms-pathogenesis
#9
Hanane Touil, Antonia Kobert, Nathalie Lebeurrier, Aja Rieger, Philippe Saikali, Caroline Lambert, Lama Fawaz, Craig S Moore, Alexandre Prat, Jennifer Gommerman, Jack P Antel, Yasuto Itoyama, Ichiro Nakashima, Amit Bar-Or
BACKGROUND: The success of clinical trials of selective B cell depletion in patients with relapsing multiple sclerosis (MS) indicates B cells are important contributors to peripheral immune responses involved in the development of new relapses. Such B cell contribution to peripheral inflammation likely involves antibody-independent mechanisms. Of growing interest is the potential that B cells, within the MS central nervous system (CNS), may also contribute to the propagation of CNS-compartmentalized inflammation in progressive (non-relapsing) disease...
April 19, 2018: Journal of Neuroinflammation
https://www.readbyqxmd.com/read/29672284/induction-of-pluripotent-stem-cell-derived-cardiomyocyte-toxicity-by-supernatant-of-long-term-stored-red-blood-cells-in-vitro
#10
Fengyan Fan, Yang Yu, Liping Sun, Shufang Wang, Rui Wang, Leiying Zhang, Cuiying Li, Deqing Wang
BACKGROUND/AIMS: Preserved red blood cells (RBCs) in vitro undergo a series of morphological, functional and metabolic changes during storage. RBC metabolites accumulate over time during storage, the toxicity of the supernatants of RBCs (SSRBCs) on tissue cells is largely unknown. Here, we aimed to study cardiomyocyte toxicity by supernatant of long term-stored RBCs in vitro and to discover elements involved in the mechanism. METHODS: Using human-induced pluripotent stem cell-derived cardiomyocytes (hiPS-CMs) and real-time cell analyzing (RTCA), we analyzed the cardiotoxicity of d0, d14 and d35 SSRBCs...
April 16, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29671926/cancer-as-a-tissue-the-significance-of-cancer-stromal-interactions-in-the-development-morphogenesis-and-progression-of-human-upper-digestive-tract-cancer
#11
REVIEW
Hiroshi Yokozaki, Yu-Ichiro Koma, Manabu Shigeoka, Mari Nishio
We review the significance of cancer-stromal interactions (CSIs) in the development, morphogenesis and progression of human gastric and esophageal cancer based on the data obtained from co-culture experiments. Orthotopic fibroblasts in the gastric cancer stroma not only promoted their growth by cancer cells but were also responsible for the mobility, morphogenesis and epithelial-to-mesenchymal transition (EMT) of the cancer cells through CSI. Bone marrow-derived mesenchymal stem cells could be part of the origin of cancer-associated fibroblasts (CAFs) of the gastric cancer providing an advantageous microenvironment for the restoration of cancer stem cells with the induction of the EMT...
April 19, 2018: Pathology International
https://www.readbyqxmd.com/read/29671574/zinc-oxide-nanoparticles-dispersed-in-ionic-liquids-show-high-antimicrobial-efficacy-to-skin-specific-bacteria
#12
Anusha Aditya, Sabyasachi Chattopadhyay, Diksha Jha, Hemant K Gautam, Souvik Maiti, Munia Ganguli
Zinc oxide nanoparticles of 60 nm were dispersed in PBS and 2 different ionic liquids- choline acetate, 1-Butyl-3-methylimidazolium chloride. These formulations were tested in 4 different strains- Escherichia coli, Bacillus cereus, Klebsiella pneumoniae, and Staphylococcus epidermidis. Out of these combinations, maximum efficiency was obtained for ZnO nanoparticle dispersed in imidazolium based ionic liquid against skin specific S. epidermidis. Through a series of experiments, it has been established that the mechanism of killing this skin-specific bacteria is through ROS production leading to bacterial cell lysis...
April 19, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29670217/bacterial-derived-exopolysaccharides-enhance-antifungal-drug-tolerance-in-a-cross-kingdom-oral-biofilm
#13
Dongyeop Kim, Yuan Liu, Raphael I Benhamou, Hiram Sanchez, Áurea Simón-Soro, Yong Li, Geelsu Hwang, Micha Fridman, David R Andes, Hyun Koo
Fungal-bacterial interactions generate unique biofilms that cause many infections in humans. Candida albicans interact with Streptococcus mutans in dental biofilms associated with severe childhood tooth-decay, a prevalent pediatric oral disease. Current modalities are ineffective and primarily based on antimicrobial monotherapies despite the polymicrobial nature of the infection. Here, we show that the combination of clinically used topical antifungal fluconazole with povidone iodine (PI) can completely suppress C...
April 18, 2018: ISME Journal
https://www.readbyqxmd.com/read/29669575/effects-of-senolytic-drugs-on-human-mesenchymal-stromal-cells
#14
Clara Grezella, Eduardo Fernandez-Rebollo, Julia Franzen, Mónica Sofia Ventura Ferreira, Fabian Beier, Wolfgang Wagner
BACKGROUND: Senolytic drugs are thought to target senescent cells and might thereby rejuvenate tissues. In fact, such compounds were suggested to increase health and lifespan in various murine aging models. So far, effects of senolytic drugs have not been analysed during replicative senescence of human mesenchymal stromal cells (MSCs). METHODS: In this study, we tested four potentially senolytic drugs: ABT-263 (navitoclax), quercetin, nicotinamide riboside, and danazol...
April 18, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29668890/luteinizing-hormone-facilitates-antral-follicular-maturation-and-survival-via-thecal-paracrine-signaling-in-cattle
#15
Katsushige Hattori, Makoto Orisaka, Shin Fukuda, Kimihisa Tajima, Yukiko Yamazaki, Tetsuya Mizutani, Yoshio Yoshida
The use of LH supplementation in assisted reproductive technology (ART) cycles improves the ongoing pregnancy rate in women with poor ovarian response (POR). However, our knowledge of the precise role of LH during the follicular phase of the menstrual cycle is incomplete. To explore the role of LH in the maturation of small antral follicles, we used an in vitro two-cell system that involved co-culturing bovine granulosa cells (GC) and theca cells (TC) on a collagen membrane. Treatment of TC with LH stimulated androgen production in TC by inducing the expression of androgenic factors, subsequently increasing estrogen biosynthesis in GC by providing androgen substrates and inducing aromatase expression...
April 13, 2018: Endocrinology
https://www.readbyqxmd.com/read/29668679/similarities-and-differences-between-helminth-parasites-and-cancer-cell-lines-in-shaping-human-monocytes-insights-into-parallel-mechanisms-of-immune-evasion
#16
Prakash Babu Narasimhan, Leor Akabas, Sameha Tariq, Naureen Huda, Sasisekhar Bennuru, Helen Sabzevari, Robert Hofmeister, Thomas B Nutman, Roshanak Tolouei Semnani
A number of features at the host-parasite interface are reminiscent of those that are also observed at the host-tumor interface. Both cancer cells and parasites establish a tissue microenvironment that allows for immune evasion and may reflect functional alterations of various innate cells. Here, we investigated how the phenotype and function of human monocytes is altered by exposure to cancer cell lines and if these functional and phenotypic alterations parallel those induced by exposure to helminth parasites...
April 18, 2018: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29668341/silicon-dioxide-nanoparticle-exposure-affects-small-intestine-function-in-an-in-vitro-model
#17
Zhongyuan Guo, Nicole J Martucci, Yizhong Liu, Eusoo Yoo, Elad Tako, Gretchen J Mahler
The use of nanomaterials to enhance properties of food and improve delivery of orally administered drugs has become common, but the potential health effects of these ingested nanomaterials remain unknown. The goal of this study is to characterize the properties of silicon dioxide (SiO2 ) nanoparticles (NP) that are commonly used in food and food packaging, and to investigate the effects of physiologically realistic doses of SiO2 NP on gastrointestinal (GI) health and function. In this work, an in vitro model composed of Caco-2 and HT29-MTX co-cultures, which represent absorptive and goblet cells, was used...
April 18, 2018: Nanotoxicology
https://www.readbyqxmd.com/read/29668079/co-culture-of-adipose-derived-stem-cells-and-chondrocytes-on-three-dimensionally-printed-bioscaffolds-for-craniofacial-cartilage-engineering
#18
Robert J Morrison, Hassan B Nasser, Khaled N Kashlan, David A Zopf, Derek J Milner, Colleen L Flanangan, Matthew B Wheeler, Glenn E Green, Scott J Hollister
OBJECTIVES/HYPOTHESIS: Reconstruction of craniofacial cartilagenous defects are among the most challenging surgical procedures in facial plastic surgery. Bioengineered craniofacial cartilage holds immense potential to surpass current reconstructive options, but limitations to clinical translation exist. We endeavored to determine the viability of utilizing adipose-derived stem cell-chondrocyte co-culture and three-dimensional (3D) printing to produce 3D bioscaffolds for cartilage tissue engineering...
April 18, 2018: Laryngoscope
https://www.readbyqxmd.com/read/29667009/encapsulating-viability-of-multi-strain-lactobacilli-as-potential-probiotic-in-pigs
#19
Sudthidol Piyadeatsoontorn, Rutjawate Taharnklaew, Tewa Upathanpreecha, Pairat Sornplang
Important aspects of the selection of probiotics to be used for mixing in animal feed include host species specificity and probiotic cell survival during production and storage of their products. The research was to screen and investigate some probiotic properties of lactic acid bacteria (LAB) isolated from pig fecal samples. One hundred and thirty-eight representative LAB isolates, which were isolated from 51 pig fecal samples, were tested for acid and bile tolerance, antimicrobial susceptibility, antibacterial activity, potential adhesion to the cell surface, and survival rates when stored in varied microencapsulation forms: freeze-dried, spray-dried, and micro-beads...
April 17, 2018: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/29666543/spoilage-of-refrigerated-litopenaeus-vannamei-eavesdropping-on-acinetobacter-acyl-homoserine-lactones-promotes-the-spoilage-potential-of-shewanella-baltica
#20
Suqin Zhu, Haohao Wu, Caili Zhang, Jinxin Jie, Zunying Liu, Mingyong Zeng, Changyun Wang
Shewanella baltica and Acinetobacter are among the predominant spoilage bacteria in refrigerated shrimp ( Litopenaeus vannamei ). S. baltica are incapable of producing acyl-homoserine lactone (AHL) quorum sensing signals, but can respond to environmental AHLs. In this paper, Acinetobacter was found to produce three AHLs, i.e. N -butanoyl-l-homoserine lactone (C4-HSL), N -(3-oxohexanoyl)-l-homoserine lactone (O-C6-HSL) and N -(3-oxooctanoyl)-l-homoserine lactone (O-C8-HSL), according to thin-layer chromatography using the bioreporter Agrobacterium tumefaciens A136...
May 2018: Journal of Food Science and Technology
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