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cartilage tissue engineering

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https://www.readbyqxmd.com/read/29055244/a-pilot-study-comparing-mechanical-properties-of-tissue-engineered-cartilages-and-various-endogenous-cartilages
#1
Andrew K Pappa, Sajjad Soleimani, Montserrat Caballero, Alexandra E Halevi, John A van Aalst
BACKGROUND: Mechanical properties of tissue-engineered cartilage and a variety of endogenous cartilage were measured. The main goal was to evaluate if the tissue-engineered cartilage have similar mechanical characteristics to be replaced with rib cartilage in microtia reconstruction. Such study lays the foundation for future human clinical trials for microtia reconstruction. METHOD: Atomic force microscopy and compression testing were used to measure the viscoelasticity of tissue-engineered cartilage (stem cell seeded on Poly lactic co-glycolytic acid nanofibers and Pellet) and endogenous cartilage: conchal bowl, microtic ears, preauricular remnants, and rib...
October 10, 2017: Clinical Biomechanics
https://www.readbyqxmd.com/read/29051809/impact-of-expansion-and-redifferentiation-under-hypothermia-on-chondrogenic-capacity-of-cultured-human-septal-chondrocytes
#2
Achim von Bomhard, Joseph Faust, Alexander F Elsaesser, Silke Schwarz, Katharina Pippich, Nicole Rotter
A critical limitation in the cultivation of cartilage for tissue engineering is the dedifferentiation in chondrocytes, mainly during in vitro amplification. Despite many previous studies investigating the influence of various conditions, no data exist concerning the effects of hypothermia. Our aim has been to influence chondrocyte dedifferentiation in vitro by hypothermic conditions. Chondrocytes were isolated from cartilage biopsies and seeded in monolayer and in three-dimensional pellet-cultures. Each cell culture was either performed at 32...
January 2017: Journal of Tissue Engineering
https://www.readbyqxmd.com/read/29048769/in-vivo-implantation-of-a-tissue-engineered-stem-cell-seeded-hemi-laryngeal-replacement-maintains-airway-phonation-and-swallowing-in-pigs
#3
P Herrmann, T Ansari, A Southgate, A Varanou Jenkins, L Partington, C Carvalho, S Janes, M Lowdell, P Sibbons, M A Birchall
BACKGROUND: Laryngeal functional impairment relating to swallowing, vocalisation and respiration can be life changing and devastating for patients. A tissue engineering approach to regenerating vocal folds would represent a significant advantage over current clinical practice. METHOD: Porcine hemi-larynx were de-cellularised under negative pressure. The resultant acellular scaffold was seeded with human bone marrow derived mesenchymal stem cells and primary human epithelial cells...
October 19, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29046163/application-of-hydrogels-in-cartilage-tissue-engineering
#4
Ximu Zhang, Wei Zhang, Maobin Yang
Cartilage has limited ability for self-repairing, prompting the search for cartilage substitutes that can repair cartilage defects. Hydrogels have attracted attention as cartilage substitutes, since their mechanical properties, swelling ability and lubricating behavior are similar to extracellular matrix of articular cartilage. Hydrogels can be of natural, synthetic or hybrid origin, and hydrogels can encapsulate stem cells and/or be loaded with growth factors to promote cell differentiation into a chondrogenic phenotype...
October 17, 2017: Current Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29043781/direct-writing-electrospinning-of-scaffolds-with-multi-dimensional-fiber-architecture-for-hierarchical-tissue-engineering
#5
Honglin Chen, Afonso de Botelho Ferreira Braga Malheiro, Clemens van Blitterswijk, Carlos Mota, Paul Andrew Wieringa, Lorenzo Moroni
Nanofibrous structures have long been used as scaffolds for tissue engineering (TE) applications, due to their favourable characteristics such as high porosity, flexibility, high cell attachment and enhanced proliferation, and overall resemblance to native extracellular matrix (ECM). Such scaffolds can be easily produced at a low cost via electrospinning (ESP), but generally cannot be fabricated with a regular and/or complex geometry, characterized by macropores and uniform thickness. We present here a novel technique for direct writing (DW) with solution ESP to produce complex three-dimensional (3D) multi-scale and ultrathin (~1 µm) fibrous scaffolds with desirable patterns and geometries...
October 18, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29039590/microrna%C3%A2-218-promotes-prostaglandin-e2-to-inhibit-osteogenic-differentiation-in-synovial-mesenchymal-stem-cells-by-targeting-15%C3%A2-hydroxyprostaglandin-dehydrogenase-nad
#6
Ruijun Cong, Kun Tao, Peiliang Fu, Lieming Lou, Yuchang Zhu, Song Chen, Xinyu Cai, Lingzhou Mao
The chondrogenic differentiation of synovial mesenchymal stem cells (SMSCs) is regulated by essential transcription factors and signaling cascades. However, the precise mechanisms involved in this process remain unclear. MicroRNAs (miRs/miRNAs) are undersized non‑coding RNAs responsible for the post‑transcriptional regulation of gene expression, by binding to the 3'‑untranslated regions (3'‑UTRs) of their target mRNAs. miRNAs may constitute a promising tool to regulate SMSC differentiation and to advance the controlled differentiation of SMSCs in therapeutic applications...
October 17, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29038440/mir-29b-regulates-expression-of-collagens-i-and-iii-in-chondrogenically-differentiating-bmsc-in-an-osteoarthritic-environment
#7
Ute Mayer, Achim Benditz, Susanne Grässel
Osteoarthritis (OA) is characterized by a slowly progressing, irreversible loss of articular cartilage. Tissue engineering approaches for cartilage regeneration include stem cell-based strategies but not much is known about their repair capacity in an OA microenvironment. The aim of the present study was to identify factors regulating collagen expression during chondrogenic differentiation of bone marrow-derived mesenchymal stem cells (BMSC) in an OA microenvironment. Coculture with OA cartilage induced miR-29b expression in BMSC which inhibited collagen I and III expression...
October 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29037894/indentation-mapping-reveals-poroelastic-but-not-viscoelastic-properties-spanning-native-zonal-articular-cartilage
#8
Joseph A Wahlquist, Frank W DelRio, Mark A Randolph, Aaron H Aziz, Chelsea M Heveran, Stephanie J Bryant, Corey P Neu, Virginia L Ferguson
Osteoarthrosis is a debilitating disease affecting millions, yet engineering materials for cartilage regeneration has proven difficult because of the complex microstructure of this tissue. Articular cartilage, like many biological tissues, produces a time-dependent response to mechanical load that is critical to cell's physiological function in part due to solid and fluid phase interactions and property variations across multiple length scales. Recreating the time-dependent strain and fluid flow may be critical for successfully engineering replacement tissues but thus far has largely been neglected...
October 13, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/29030197/green-synthesis-of-degradable-conductive-thermosensitive-oligopyrrole-chitosan-hydrogel-intended-for-cartilage-tissue-engineering
#9
Mana Kashi, Fatemeh Baghbani, Fathollah Moztarzadeh, Hamid Mobasheri, Elaheh Kowsari
Electroactive scaffolds containing conductive polymers can promote tissue repair and regeneration. However, these polymers are non-degradable and cannot be removed from body. To overcome this limitation of conductive polymers, we developed a novel injectable electroactive hydrogel containing pyrrole oligomers which possessed the unique properties of being both electrically conductive and biodegradable. First, pyrrole oligomers were synthesized via chemical polymerization and were found to be amorphous with a non-globular morphology...
October 10, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29027284/polydopamine-particles-reinforced-poly-vinyl-alcohol-hydrogel-with-nir-light-triggered-shape-memory-and-self-healing-capability
#10
Li Yang, Zhanhua Wang, Guoxia Fei, Hesheng Xia
This study focuses on developing a facile approach to prepare biocompatible poly(vinyl alcohol) (PVA) composite hydrogels containing polydopamine particles (PDAPs) with ultrafast near-infrared (NIR) light-triggered shape memory and self-healing capability. The PVA-PDAPs composite hydrogels with excellent mechanical properties can be achieved after freezing/thawing treatment, and the formation of physically cross-linked networks from the hydrogen bonding (H-bonding) between PVA and PDAPs. Due to the excellent photothermal effect of polydopamine, the composited hydrogel can achieve rapid shape recovery and efficient self-healing properties under NIR light exposure in a short time...
October 13, 2017: Macromolecular Rapid Communications
https://www.readbyqxmd.com/read/29020880/pulsed-electromagnetic-fields-and-tissue-engineering-of-the-joints
#11
Kenjiro Iwasa, Reddi A Hari
BACKGROUND: Bone and cartilage joint formation, maintenance and regeneration are regulated by both chemical and physical signals. Among the physical signals there is increasing realization of the role of Pulsed electromagnetic fields (PEMF) in the treatment of nonunions of bone fractures. The discovery of the piezoelectric properties of bone by Fukada and Yasuda in 1953 in Japan established the foundation of this field. Pioneering research by Bassett and Brighton and their teams resulted in the approval by Food and Drug Administration (FDA) the use of PEMF in treatment of fracture healing...
October 12, 2017: Tissue Engineering. Part B, Reviews
https://www.readbyqxmd.com/read/29017973/modulating-microfibrillar-alignment-and-growth-factor-stimulation-to-regulate-mesenchymal-stem-cell-differentiation
#12
Dinorath Olvera, Binulal N Sathy, Simon F Carroll, Daniel J Kelly
The ideal tissue engineering (TE) strategy for ligament regeneration should recapitulate the bone - calcified cartilage - fibrocartilage - soft tissue interface. Aligned electrospun-fibers have been shown to guide the deposition of a highly organized extracellular matrix (ECM) necessary for ligament TE. However, recapitulating the different tissues observed in the bone-ligament interface using such constructs remains a challenge. This study aimed to explore how fiber alignment and growth factor stimulation interact to regulate the chondrogenic and ligamentous differentiation of mesenchymal stem cells (MSCs)...
October 7, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28992510/three-dimensional-piezoelectric-fibrous-scaffolds-selectively-promote-mesenchymal-stem-cell-differentiation
#13
Sita M Damaraju, Yueyang Shen, Ezinwa Elele, Boris Khusid, Ahmad Eshghinejad, Jiangyu Li, Michael Jaffe, Treena Livingston Arinzeh
The discovery of electric fields in biological tissues has led to efforts in developing technologies utilizing electrical stimulation for therapeutic applications. Native tissues, such as cartilage and bone, exhibit piezoelectric behavior, wherein electrical activity can be generated due to mechanical deformation. Yet, the use of piezoelectric materials have largely been unexplored as a potential strategy in tissue engineering, wherein a piezoelectric biomaterial acts as a scaffold to promote cell behavior and the formation of large tissues...
September 19, 2017: Biomaterials
https://www.readbyqxmd.com/read/28990462/atmps-a-guide-for-bone-marrow-derived-mscs-application-in-bone-and-cartilage-tissue-engineering
#14
Davide Confalonieri, Andrea Schwab, Heike Walles, Franziska Ehlicke
Millions of people worldwide suffer from trauma- or age-related orthopedic diseases such as osteoarthritis, osteoporosis or cancer. Tissue Engineering and Regenerative Medicine are multidisciplinary fields focusing on the development of artificial organs, biomimetic engineered tissues and cells to restore or maintain tissue and organ function. While allogenic and future autologous transplantations are nowadays the gold standards for both cartilage and bone defect repair, they are both subject to important limitations such as availability of healthy tissue, donor site morbidity and graft rejection...
October 7, 2017: Tissue Engineering. Part B, Reviews
https://www.readbyqxmd.com/read/28988692/the-survey-on-cellular-and-tissue-engineered-therapies-in-europe-and-neighboring-eurasian-countries-in-2014-and-2015
#15
Hilary Ireland, Max H P Gay, Helen Baldomero, Barbara De Angelis, Hossein Baharvand, Mark W Lowdell, Jakob Passweg, Ivan Martin
BACKGROUND AIMS: With the support of five established scientific organizations, this report, the seventh of its kind, describes activity in Europe for the years 2014 and 2015 in the area of cellular and tissue-engineered therapies, excluding hematopoietic stem cell (HSC) treatments for the reconstitution of hematopoiesis. METHODS: In 2015 [respectively 2014], 205 [276] teams from 32 countries responded to the cellular and tissue-engineered therapy survey; 178 [126] teams reported treating 3686 [2665] patients...
October 4, 2017: Cytotherapy
https://www.readbyqxmd.com/read/28975895/pla-short-i-sub-micron-fibers-i-reinforcement-of-3d-bioprinted-alginate-constructs-for-cartilage-regeneration
#16
Alicja Kosik-Kozioł, Marco Costantini, Tomasz Bolek, Krisztina Szoke, Andrea Barbetta, Jan E Brinchmann, Wojciech Święszkowski
In this study, we present an innovative strategy to reinforce 3D printed hydrogel constructs for cartilage tissue engineering by formulating composite bioinks containing alginate and short sub-micron polylactide (PLA) fibers. We demonstrate that Young's modulus obtained for pristine alginate constructs (6.9 ± 1.7 kPa) can be increased threefold (up to 25.1 ± 3.8 kPa) with the addition of PLA short fibers. Furthermore, to assess the performance of such materials in cartilage tissue engineering, we loaded the bioinks with human chondrocytes and cultured in vitro the bioprinted constructs for up to 14 days...
October 4, 2017: Biofabrication
https://www.readbyqxmd.com/read/28975658/current-and-novel-injectable-hydrogels-to-treat-focal-chondral-lesions-properties-and-applicability
#17
REVIEW
Cecilia Pascual-Garrido, Francisco Rodriguez-Fontan, Elizabeth A Aisenbrey, Karin A Payne, Jorge Chahla, Laurie R Goodrich, Stephanie J Bryant
Focal chondral lesions and early osteoarthritis (OA) are responsible for progressive joint pain and disability in millions of people worldwide, yet there is currently no surgical joint preservation treatment available to fully restore the long term functionality of cartilage. Limitations of current treatments for cartilage defects have prompted the field of cartilage tissue engineering, which seeks to integrate engineering and biological principles to promote the growth of new cartilage to replace damaged tissue...
October 3, 2017: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://www.readbyqxmd.com/read/28974254/synergistic-effects-on-mesenchymal-stem-cell-based-cartilage-regeneration-by-chondrogenic-preconditioning-and-mechanical-stimulation
#18
Sien Lin, Wayne Yuk Wai Lee, Qian Feng, Liangliang Xu, Bin Wang, Gene Chi Wai Man, Yuanfeng Chen, Xiaohua Jiang, Liming Bian, Liao Cui, Bo Wei, Gang Li
BACKGROUND: Mesenchymal stem cells (MSCs) hold promising translational potential in cartilage regeneration. However, the efficacy of MSC-based tissue engineering is not satisfactory in the treatment of cartilage defect because of the inevitable cellular functional changes during ex vivo cell expansion. How to maintain the chondrogenic capacity of MSCs to improve their therapeutic outcomes remains an outstanding question. METHODS: Bone marrow-derived MSCs were firstly primed in chondrogenic induction medium which was then replaced with normal growth medium to attain the manipulated cells (M-MSCs)...
October 3, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28969580/the-potential-for-synovium-derived-stem-cells-in-cartilage-repair
#19
Eva Johanna Kubosch, Gernot Michael Lang, David Fürst, David Christopher Kubosch, Kaywan Izadpanah, Bernd Rolauffs, Norbert P Südkamp, Hagen Schmal
BACKGROUND: Articular cartilage defects often result in pain, loss of function and finally osteoarthritis. Developing cell-based therapies for cartilage repair is a major goal of orthopaedic research. Autologous chondrocyte implantation is currently the gold standard cell-based surgical procedure for the treatment of large, isolated, full thickness cartilage defects. Several disadvantages such as the need for two surgical procedures or hypertrophic regenerative cartilage, underline the need for alternative cell sources...
October 2, 2017: Current Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28967913/mechanical-confinement-regulates-cartilage-matrix-formation-by-chondrocytes
#20
Hong-Pyo Lee, Luo Gu, David J Mooney, Marc E Levenston, Ovijit Chaudhuri
Cartilage tissue equivalents formed from hydrogels containing chondrocytes could provide a solution for replacing damaged cartilage. Previous approaches have often utilized elastic hydrogels. However, elastic stresses may restrict cartilage matrix formation and alter the chondrocyte phenotype. Here we investigated the use of viscoelastic hydrogels, in which stresses are relaxed over time and which exhibit creep, for three-dimensional (3D) culture of chondrocytes. We found that faster relaxation promoted a striking increase in the volume of interconnected cartilage matrix formed by chondrocytes...
October 2, 2017: Nature Materials
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