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ANA nucleolar pattern

Bharat Kumar, Jennifer Strouse, Melissa Swee, Petar Lenert, Manish Suneja
INTRODUCTION: Hydralazine is an antihypertensive medication that has been associated with drug-induced lupus erythematosus (DIL) as well as ANCA-associated vasculitis (AAV). Although rare, early diagnosis is critical since drug cessation is the mainstay of therapy. This retrospective study aims to characterize the clinical, laboratory, and histopathologic features of this disease. METHODS: Once approval was obtained from the Institutional Review Board at the University of Iowa, all patients carrying a diagnosis of vasculitis (ICD9 code: 447...
January 12, 2018: Seminars in Arthritis and Rheumatism
Kun Men, Yu Chen, Jingwei Zhang, Dianjun Wei
Background/Aims: To evaluate cellular immune function in systemic lupus erythematosus (SLE) patients over 60 years old, the association between antinuclear antibody (ANA) titers and the ratio of CD4+ /CD8+ was analyzed in this study. The distribution of ANAs and extractable nuclear antibodies (ENAs) in a healthy elderly population was also investigated. Methods: Serum ANA titers were assayed by indirect immunofluorescence (IIF) and the CD4+ /CD8+ T-cell ratio was determined by flow cytometry in 76 SLE patients and 30 healthy control individuals...
November 29, 2017: Korean Journal of Internal Medicine
Yuhei Ito, Machiko Arita, Shogo Kumagai, Reoto Takei, Maki Noyama, Fumiaki Tokioka, Keisuke Nishimura, Takashi Koyama, Kenji Notohara, Tadashi Ishida
BACKGROUND: To identify the prognostic factors for survival in patients with interstitial pneumonia with autoimmune features (IPAF) who meet the serological domain of the IPAF criteria. METHODS: We retrospectively analysed 99 IPAF patients who met the serological domain and were hospitalised at the Respiratory Medicine Unit of Kurashiki Central Hospital from 1999 to 2015. The high-resolution computed tomography findings were usual interstitial pneumonia (UIP; n = 1), non-specific interstitial pneumonia (NSIP; n = 63), NSIP with organizing pneumonia (OP) overlap (n = 15), and OP (n = 20)...
August 14, 2017: BMC Pulmonary Medicine
Marcia Cristina de Souza Lara Kamei, Lucas Baumgärtner, Suzana Paiva, Cláudio Henrique Zawadzki, Isabel Cristina Martins-Santos, Ana Luiza de Brito Portela-Castro
Hypostomus shows wide morphological patterns, corroborated by great chromosomal diversity that has suggested the existence of new species, especially from small tributaries. Cytogenetic analysis has contributed to estimate a cryptic diversity providing important data for taxonomic and evolutionary studies. Cytogenetic techniques were carried out on species from a small tributary of Ivaí River, Keller River (upper Paraná River basin): Hypostomus aff. ancistroides, Hypostomus topavae, and Hypostomus aff. hermanni that presented 2n = 68, 80, and 72 chromosomes, respectively...
August 2017: Zebrafish
Bing Zheng, Enling Li, Haoming Zhu, Jingbo Lu, Xinming Shi, Jie Zhang, Min Li
BACKGROUND: Indirect immunofluorescence (IIF) assays are recommended as the gold standard method for the detection of antinuclear antibodies (ANAs). This study aimed to investigate the reliability of an automated system. METHODS: We compared 3745 serum samples using NOVA View archived images with manual analysis via microscopy. A custom cutoff value was established to distinguish ANA titers and was validated in two clinical laboratories. The automatic ANA pattern recognition system was evaluated, and all ANA-positive sera were subjected to two commercial ANA IIF kits to compare the consistency of the pattern interpretation results...
October 26, 2017: Clinical Chemistry and Laboratory Medicine: CCLM
María Laura Alberti, Francisco Paulin, Heidegger Mateos Toledo, Martín Eduardo Fernández, Fabián Matías Caro, Jorge Rojas-Serrano, Mayra Edith Mejía
OBJECTIVES: To identify clinical or immunological features in patients with undifferentiated connective tissue disease (UCTD) associated interstitial lung disease (ILD), in order to group them and recognize different functional and high resolution computed tomography (HRCT) behavior. METHODS: Retrospective cohort study. Patients meeting Kinder criteria for UCTD were included. We defined the following predictive variables: 'highly specific' connective tissue disease (CTD) manifestations (Raynaud's phenomenon, dry eyes or arthritis), high antinuclear antibody (ANA) titer (above 1: 320), and 'specific' ANA staining patterns (centromere, cytoplasmic and nucleolar patterns)...
March 2018: Reumatología Clinica
Chelsea Bentow, Marvin J Fritzler, Eckart Mummert, Michael Mahler
PURPOSE: The dense fine speckled (DFS) pattern as detected by indirect immunofluorescence (IIF) on HEp-2 cells has been associated with several inflammatory diseases but is most commonly observed in individuals that do not have an antinuclear antibody (ANA)-associated rheumatic disease and even in apparently healthy individuals. Consequently, the accurate identification and correct reporting of this IIF pattern is of utmost importance and accordingly has been recognized by several international study groups for the detection of ANA...
December 2016: Auto- Immunity Highlights
Nobuhide Hayashi, Jun Saegusa, Kenichi Uto, Chinami Oyabu, Toshiharu Saito, Itsuko Sato, Seiji Kawano, Shunichi Kumagai
Antinuclear antibody (ANA) testing is indispensable for diagnosing and understanding clinical conditions of autoimmune diseases. The indirect immunofluorescence assay (IFA) is the gold standard for ANA screening, and it can detect more than 100 different antibodies, such as anti-PCNA as well as anti-cytoplasmic antibodies. However, complicated procedures of conventional IFA and visual interpretation require highly skilled laboratory staff. This study evaluates the capability, characteristics, and applicability of the recently developed ANA detection system (EUROPattern Cosmic IFA System, EPA) using HEp20-10 cells and the automated pattern recognition microscope...
February 2016: Rinsho Byori. the Japanese Journal of Clinical Pathology
C Bentow, R Rosenblum, P Correia, E Karayev, D Karayev, D Williams, J Kulczycka, M J Fritzler, M Mahler
OBJECTIVE: Antinuclear antibodies (ANA) represent a hallmark in the diagnosis of ANA-associated rheumatic diseases (AARD). However, anti-DFS70 antibodies are present in a higher portion of the healthy individuals (HI) than in patients with AARD. Consequently, we developed a novel, highly specific indirect immunofluorescence (IIF) method that blocks anti-DFS70 antibodies from binding to HEp-2 cells and to evaluate the method in a multi-center study. METHODS: A total of 18 samples from systemic lupus erythematosus patients (SLE, n = 7) and HI (n = 11) were used for the initial development of the immunoadsorption method...
July 2016: Lupus
Chanwit Prapinjumrune, Chalakorn Prucktrakul, Trakarn Sooktonglarng, Kobkan Thongprasom
OBJECTIVE: This study investigated the presence of antinuclear antibody (ANA) in older Thais compared with middle-age and younger participants. BACKGROUND: Antinuclear antibody represents the first step in the diagnostic testing for lupus erythematosus (LE) and other autoimmune diseases. Due to the lack of reference ANA levels in older, middle-age and younger Thais healthy participants, this study will be useful for determining the proper diagnostic and treatment criteria...
May 12, 2016: Gerodontology
C M Cabrera, E Fernández-Grande, J M Urra
Nucleolar staining of antinuclear antibodies (ANAs) is not exclusive to patients suffering systemic sclerosis (SSc) since it can occur in other autoimmune diseases, such as systemic lupus erythematosus (SLE). The nucleolar ANA pattern presents a low incidence in patients with SLE, with less than 9% reported in some studies. The significance of nucleolar staining and antinucleolar antibodies (ANoA) in SLE is still unknown, as is its association with clinical manifestations. To address these issues, a case-control study was carried out...
August 2016: Lupus
Chung-Chuan Cheng, Chun-Feng Lu, Tsu-Yi Hsieh, Yaw-Jen Lin, Jin-Shiuh Taur, Yung-Fu Chen
Indirect immunofluorescence technique applied on HEp-2 cell substrates provides the major screening method to detect ANA patterns in the diagnosis of autoimmune diseases. Currently, the ANA patterns are mostly inspected by experienced physicians to identify abnormal cell patterns. The objective of this study is to design a computer-assisted system to automatically detect cell patterns of IIF images for the diagnosis of autoimmune diseases in the clinical setting. The system simulates the functions of modern flow cytometer and provides the diagnostic reports generated by the system to the technicians and physicians through the radar graphs, box-plots, and tables...
October 2015: Journal of Medical Systems
Anirban Ghosh, Avinandan Banerjee, Amlan Kanti Biswas
AIM: This study was undertaken to find out the characteristics of clinical, radiological and functional changes affecting the respiratory system in patients with systemic sclerosis (SSc) from eastern India, and the association of these characteristics with pulmonary hypertension. METHODS: This was a cross-sectional, observational study involving 46 patients. Other than the routine tests, anti-nuclear antibody (ANA), spirometry, diffusing capacity of lung for carbon monoxide (DLCO) measurement, chest radiograph, high-resolution computed tomography (HRCT) of thorax, 6-minute walk test and echocardiography were done...
October 2014: Indian Journal of Chest Diseases & Allied Sciences
Gerald Schaefer, Niraj P Doshi, Shao Ying Zhu, Qinghua Hu
Indirect immunofluorescence imaging is employed to identify antinuclear antibodies in HEp-2 cells which founds the basis for diagnosing autoimmune diseases and other important pathological conditions involving the immune system. Six categories of HEp-2 cells are generally considered, namely homogeneous, fine speckled, coarse speckled, nucleolar, cyto-plasmic, and centromere cells. Typically, this categorisation is performed manually by an expert and is hence both time consuming and subjective. In this paper, we present a method for automatically classifiying HEp-2 cells using texture information in conjunction with a suitable classification system...
2014: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
Jinhui Hu, Yuyan Zhang, Xinghui Liu, Li Wei, Denghai Zhang
OBJECTIVE: This study investigated some T helper cytokines' association with different ANA positive sera. METHODS: Sera of 53 ANA-positive subjects (11 were stained of homogeneous pattern, 12 of speckled particle pattern, 9 of nucleolar pattern, 15 of centromere pattern, and 6 of the peripheral nuclear type) were collected and analyzed by ELISA for related cytokines and concentrations were compared with each other and 16 healthy donors. RESULTS: Investigated cytokines including IL-12(p70) and IFN-γ varied differently in the ANA sera...
February 2015: International Immunopharmacology
Ana Paula Alves-Silva, Jorge Abdala Dergam
The family Cichlidae is one of the most species-rich taxa in the Neotropics. However, the factors that determine these high levels of biodiversity remain unexplored. We have analyzed the morphological, cytogenetic, and molecular data from 62 specimens of a widespread cichlid, Geophagus brasiliensis, from three adjacent basins in southeastern Brazil. Morphological analyses did not show differences among specimens. The cytogenetic data indicate the occurrence of multiple nucleolar organizer regions and four sympatric karyotypes that differ in the first pair of chromosome morphology, in the Doce River Basin; whereas the karyotype from the Paraíba do Sul Basin is widely divergent...
February 2015: Zebrafish
R Hira-Kazal, P Shea-Simonds, J L Peacock, J Maher
Anti-nuclear antibody (ANA) testing assists in the diagnosis of several immune-mediated disorders. The gold standard method for detection of these antibodies is by indirect immunofluorescence testing on human epidermoid laryngeal carcinoma (HEp-2) cells. However, many laboratories test for these antibodies using solid-phase assays such as enzyme-linked immunosorbent assay (ELISA), which allows for higher throughput testing at reduced cost. In this study, we have audited the performance of a previously established ELISA assay to screen for ANA, making comparison with the gold standard HEp-2 immunofluorescence test...
April 2015: Clinical and Experimental Immunology
M Scolnik, E Lancioni, C Saucedo, J Marin, M Sabelli, Z Bedran, E R Soriano, L J Catoggio
OBJECTIVES: Prevalence of systemic sclerosis (SSc) and different clinical subsets varies across the world. Few data have been published on SSc patients in Latin America. Our objective was to describe a SSc cohort in Argentina and to compare clinical findings, disease subsets and antibodies with other international SSc populations. METHODS: Patients with SSc (n=234) seen at the Rheumatology section of the Hospital Italiano de Buenos Aires between 2000-2011 were retrospectively analysed...
November 2014: Clinical and Experimental Rheumatology
Chikashi Terao, Koichiro Ohmura, Ryo Yamada, Takahisa Kawaguchi, Masakazu Shimizu, Yasuharu Tabara, Meiko Takahashi, Kazuya Setoh, Takeo Nakayama, Shinji Kosugi, Akihiro Sekine, Fumihiko Matsuda, Tsuneyo Mimori
OBJECTIVE: While antinuclear antibodies (ANAs) are observed in healthy populations as well as in patients with autoimmune diseases such as systemic lupus erythematosus (SLE), the detailed genetic background of ANAs has remained unclear. We undertook this study to identify the genetic determinants of ANAs in the general population in order to elucidate the underlying mechanisms of ANA production and to distinguish disease susceptibility genes from ANA production genes. METHODS: A total of 9,575 Japanese volunteers were registered, and their ANA levels were quantified using indirect immunofluorescence to analyze correlates of ANA positivity...
December 2014: Arthritis & Rheumatology
Paulo Luiz Carvalho Francescantonio, Wilson de Melo Cruvinel, Alessandra Dellavance, Luis Eduardo Coelho Andrade, Ben Hur Taliberti, Carlos Alberto von Mühlen, Carlos David Araújo Bichara, Cleonice Bueno, Cristóvão Luis Pitangueira Mangueira, Darlene Gonçalves Carvalho, Eloísa S D de O Bonfá, Fabiano de Almeida Brito, Flávia Ikeda e Araújo, Jozelia Rêgo, Kaline Medeiros Costa Pereira, Lisiane Maria Enriconi dos Anjos, Maria de Fatima Bissoli, Mittermayer Barreto Santiago, Natalya Zaidan Maluf, Rossana Rassi Alvarenga, Suzane Pretti Figueiredo Neves, Valeria Valim, Wilton Silva dos Santos
OBJECTIVE: The Fourth Brazilian Consensus for Autoantibodies Screening in HEp-2 Cells (ANA) was held in Vitória, Espírito Santo, and aimed to discuss strategies and recommendations about the technique, standardization, interpretation and quality control of the indirect immunofluorescence reaction on HEp-2 cells. METHODS: Twenty three ANA experts from university centers and private laboratories in different areas from Brazil discussed and agreed upon recommendations for the fourth edition of the Brazilian Consensus for Autoantibodies Screening in HEp-2 Cells...
January 2014: Revista Brasileira de Reumatologia
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