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ANA nucleolar pattern

Chelsea Bentow, Marvin J Fritzler, Eckart Mummert, Michael Mahler
PURPOSE: The dense fine speckled (DFS) pattern as detected by indirect immunofluorescence (IIF) on HEp-2 cells has been associated with several inflammatory diseases but is most commonly observed in individuals that do not have an antinuclear antibody (ANA)-associated rheumatic disease and even in apparently healthy individuals. Consequently, the accurate identification and correct reporting of this IIF pattern is of utmost importance and accordingly has been recognized by several international study groups for the detection of ANA...
December 2016: Auto- Immunity Highlights
Nobuhide Hayashi, Jun Saegusa, Kenichi Uto, Chinami Oyabu, Toshiharu Saito, Itsuko Sato, Seiji Kawano, Shunichi Kumagai
Antinuclear antibody (ANA) testing is indispensable for diagnosing and understanding clinical conditions of autoimmune diseases. The indirect immunofluorescence assay (IFA) is the gold standard for ANA screening, and it can detect more than 100 different antibodies, such as anti-PCNA as well as anti-cytoplasmic antibodies. However, complicated procedures of conventional IFA and visual interpretation require highly skilled laboratory staff. This study evaluates the capability, characteristics, and applicability of the recently developed ANA detection system (EUROPattern Cosmic IFA System, EPA) using HEp20-10 cells and the automated pattern recognition microscope...
February 2016: Rinsho Byori. the Japanese Journal of Clinical Pathology
C Bentow, R Rosenblum, P Correia, E Karayev, D Karayev, D Williams, J Kulczycka, M J Fritzler, M Mahler
OBJECTIVE: Antinuclear antibodies (ANA) represent a hallmark in the diagnosis of ANA-associated rheumatic diseases (AARD). However, anti-DFS70 antibodies are present in a higher portion of the healthy individuals (HI) than in patients with AARD. Consequently, we developed a novel, highly specific indirect immunofluorescence (IIF) method that blocks anti-DFS70 antibodies from binding to HEp-2 cells and to evaluate the method in a multi-center study. METHODS: A total of 18 samples from systemic lupus erythematosus patients (SLE, n = 7) and HI (n = 11) were used for the initial development of the immunoadsorption method...
July 2016: Lupus
Chanwit Prapinjumrune, Chalakorn Prucktrakul, Trakarn Sooktonglarng, Kobkan Thongprasom
OBJECTIVE: This study investigated the presence of antinuclear antibody (ANA) in older Thais compared with middle-age and younger participants. BACKGROUND: Antinuclear antibody represents the first step in the diagnostic testing for lupus erythematosus (LE) and other autoimmune diseases. Due to the lack of reference ANA levels in older, middle-age and younger Thais healthy participants, this study will be useful for determining the proper diagnostic and treatment criteria...
May 12, 2016: Gerodontology
C M Cabrera, E Fernández-Grande, J M Urra
Nucleolar staining of antinuclear antibodies (ANAs) is not exclusive to patients suffering systemic sclerosis (SSc) since it can occur in other autoimmune diseases, such as systemic lupus erythematosus (SLE). The nucleolar ANA pattern presents a low incidence in patients with SLE, with less than 9% reported in some studies. The significance of nucleolar staining and antinucleolar antibodies (ANoA) in SLE is still unknown, as is its association with clinical manifestations. To address these issues, a case-control study was carried out...
August 2016: Lupus
Chung-Chuan Cheng, Chun-Feng Lu, Tsu-Yi Hsieh, Yaw-Jen Lin, Jin-Shiuh Taur, Yung-Fu Chen
Indirect immunofluorescence technique applied on HEp-2 cell substrates provides the major screening method to detect ANA patterns in the diagnosis of autoimmune diseases. Currently, the ANA patterns are mostly inspected by experienced physicians to identify abnormal cell patterns. The objective of this study is to design a computer-assisted system to automatically detect cell patterns of IIF images for the diagnosis of autoimmune diseases in the clinical setting. The system simulates the functions of modern flow cytometer and provides the diagnostic reports generated by the system to the technicians and physicians through the radar graphs, box-plots, and tables...
October 2015: Journal of Medical Systems
Anirban Ghosh, Avinandan Banerjee, Amlan Kanti Biswas
AIM: This study was undertaken to find out the characteristics of clinical, radiological and functional changes affecting the respiratory system in patients with systemic sclerosis (SSc) from eastern India, and the association of these characteristics with pulmonary hypertension. METHODS: This was a cross-sectional, observational study involving 46 patients. Other than the routine tests, anti-nuclear antibody (ANA), spirometry, diffusing capacity of lung for carbon monoxide (DLCO) measurement, chest radiograph, high-resolution computed tomography (HRCT) of thorax, 6-minute walk test and echocardiography were done...
October 2014: Indian Journal of Chest Diseases & Allied Sciences
Gerald Schaefer, Niraj P Doshi, Shao Ying Zhu, Qinghua Hu
Indirect immunofluorescence imaging is employed to identify antinuclear antibodies in HEp-2 cells which founds the basis for diagnosing autoimmune diseases and other important pathological conditions involving the immune system. Six categories of HEp-2 cells are generally considered, namely homogeneous, fine speckled, coarse speckled, nucleolar, cyto-plasmic, and centromere cells. Typically, this categorisation is performed manually by an expert and is hence both time consuming and subjective. In this paper, we present a method for automatically classifiying HEp-2 cells using texture information in conjunction with a suitable classification system...
2014: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
Jinhui Hu, Yuyan Zhang, Xinghui Liu, Li Wei, Denghai Zhang
OBJECTIVE: This study investigated some T helper cytokines' association with different ANA positive sera. METHODS: Sera of 53 ANA-positive subjects (11 were stained of homogeneous pattern, 12 of speckled particle pattern, 9 of nucleolar pattern, 15 of centromere pattern, and 6 of the peripheral nuclear type) were collected and analyzed by ELISA for related cytokines and concentrations were compared with each other and 16 healthy donors. RESULTS: Investigated cytokines including IL-12(p70) and IFN-γ varied differently in the ANA sera...
February 2015: International Immunopharmacology
Ana Paula Alves-Silva, Jorge Abdala Dergam
The family Cichlidae is one of the most species-rich taxa in the Neotropics. However, the factors that determine these high levels of biodiversity remain unexplored. We have analyzed the morphological, cytogenetic, and molecular data from 62 specimens of a widespread cichlid, Geophagus brasiliensis, from three adjacent basins in southeastern Brazil. Morphological analyses did not show differences among specimens. The cytogenetic data indicate the occurrence of multiple nucleolar organizer regions and four sympatric karyotypes that differ in the first pair of chromosome morphology, in the Doce River Basin; whereas the karyotype from the Paraíba do Sul Basin is widely divergent...
February 2015: Zebrafish
R Hira-Kazal, P Shea-Simonds, J L Peacock, J Maher
Anti-nuclear antibody (ANA) testing assists in the diagnosis of several immune-mediated disorders. The gold standard method for detection of these antibodies is by indirect immunofluorescence testing on human epidermoid laryngeal carcinoma (HEp-2) cells. However, many laboratories test for these antibodies using solid-phase assays such as enzyme-linked immunosorbent assay (ELISA), which allows for higher throughput testing at reduced cost. In this study, we have audited the performance of a previously established ELISA assay to screen for ANA, making comparison with the gold standard HEp-2 immunofluorescence test...
April 2015: Clinical and Experimental Immunology
M Scolnik, E Lancioni, C Saucedo, J Marin, M Sabelli, Z Bedran, E R Soriano, L J Catoggio
OBJECTIVES: Prevalence of systemic sclerosis (SSc) and different clinical subsets varies across the world. Few data have been published on SSc patients in Latin America. Our objective was to describe a SSc cohort in Argentina and to compare clinical findings, disease subsets and antibodies with other international SSc populations. METHODS: Patients with SSc (n=234) seen at the Rheumatology section of the Hospital Italiano de Buenos Aires between 2000-2011 were retrospectively analysed...
November 2014: Clinical and Experimental Rheumatology
Chikashi Terao, Koichiro Ohmura, Ryo Yamada, Takahisa Kawaguchi, Masakazu Shimizu, Yasuharu Tabara, Meiko Takahashi, Kazuya Setoh, Takeo Nakayama, Shinji Kosugi, Akihiro Sekine, Fumihiko Matsuda, Tsuneyo Mimori
OBJECTIVE: While antinuclear antibodies (ANAs) are observed in healthy populations as well as in patients with autoimmune diseases such as systemic lupus erythematosus (SLE), the detailed genetic background of ANAs has remained unclear. We undertook this study to identify the genetic determinants of ANAs in the general population in order to elucidate the underlying mechanisms of ANA production and to distinguish disease susceptibility genes from ANA production genes. METHODS: A total of 9,575 Japanese volunteers were registered, and their ANA levels were quantified using indirect immunofluorescence to analyze correlates of ANA positivity...
December 2014: Arthritis & Rheumatology
Paulo Luiz Carvalho Francescantonio, Wilson de Melo Cruvinel, Alessandra Dellavance, Luis Eduardo Coelho Andrade, Ben Hur Taliberti, Carlos Alberto von Mühlen, Carlos David Araújo Bichara, Cleonice Bueno, Cristóvão Luis Pitangueira Mangueira, Darlene Gonçalves Carvalho, Eloísa S D de O Bonfá, Fabiano de Almeida Brito, Flávia Ikeda e Araújo, Jozelia Rêgo, Kaline Medeiros Costa Pereira, Lisiane Maria Enriconi dos Anjos, Maria de Fatima Bissoli, Mittermayer Barreto Santiago, Natalya Zaidan Maluf, Rossana Rassi Alvarenga, Suzane Pretti Figueiredo Neves, Valeria Valim, Wilton Silva dos Santos
OBJECTIVE: The Fourth Brazilian Consensus for Autoantibodies Screening in HEp-2 Cells (ANA) was held in Vitória, Espírito Santo, and aimed to discuss strategies and recommendations about the technique, standardization, interpretation and quality control of the indirect immunofluorescence reaction on HEp-2 cells. METHODS: Twenty three ANA experts from university centers and private laboratories in different areas from Brazil discussed and agreed upon recommendations for the fourth edition of the Brazilian Consensus for Autoantibodies Screening in HEp-2 Cells...
January 2014: Revista Brasileira de Reumatologia
Zafer Mengeloglu, Tekin Tas, Esra Kocoglu, Gülali Aktas, Seyda Karabörk
BACKGROUND AND OBJECTIVES: Autoantibodies are immunglobulins occurred directly against autoantigens that are known as endogen antigens. Autoimmune disease is an occasion that the body begins a fight against its own cells and tissues. The antibodies that are created by the body against its own cell nuclei are called as anti-nuclear antibodies (ANA), and one of the methods used for detection and pattern of ANA is indirect immunofluorescence test (IIF). In the present study, it was aimed to determine the rate of ANA positivity and patterns of the positive specimens, and to investigate the relationship between ANA positivity and diseases in patients...
March 2014: Pakistan Journal of Medical Sciences Quarterly
D Almeida González, C Casañas Rodríguez, L Magdalena Armas, A Roces Varela, I Marcelino Rodríguez, M Troche Duarte, A Cabrera de León
Anti-Ro52 (Ro52) and anti-Ro60 (Ro60) antibodies are associated with different clinical entities. We investigated their relationship with the presence of anti-SS-B/La (SSB) antibody, the pattern and titer of antinuclear antibody (ANA), and the variations in antibody profiles related with anti-SS-A/Ro (SSA) positivity. Our aim was to develop a strategy to increase the efficiency of anti-extractable nuclear antigen (ENA) determinations. Statistical analyses were based on the Chi-squared test for categorical variables, the Mann-Whitney U test to compare profiles, and the odds ratio (OR) and 95% confidence interval (95% CI) to estimate the risk of variability...
September 2014: Immunology Letters
Asli Gamze Sener, Ilhan Afsar, Mustafa Demirci
The presence of antinuclear antibodies (ANAs), directed against intracellular antigens, is a hallmark of systemic autoimmune rheumatic diseases. The indirect immunofluorescence (IIF) assay is among the most commonly used routine methods for ANA detection as the screening test. The objective of the study was to evaluate ANA patterns in a 4-year period retrospectively. All 19 996 serum samples that were sent to the Laboratory of Medical Microbiology of the tertiary Hospital by any hospital department between 1 January 2009 and 1 January 2013 with a request to test for ANA, anti-ENA or both were included in the study...
December 2014: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
Thais Lemos de Mattos, Ana Carolina Coelho, Carlos Henrique Schneider, David Otávio Carmo Telles, Marcelo Menin, Maria Claudia Gross
BACKGROUND: Hypsiboas species have been divided into seven groups using morphological and genetic characters, but for most of the species, there is no cytogenetic information available. A cytogenetic analysis using conventional staining, C-banding, silver staining, and fluorescence in situ hybridization (FISH) with telomeric sequence probes were used to investigate the karyotype of seven Amazon species of the genus Hypsiboas belonging to the following intrageneric groups: H. punctatus (H...
2014: BMC Genetics
Tatiane R Sampaio, Larissa B Pires, Renata da Rosa, Ana Lúcia Dias
The nucleolus is an important nuclear structure where transcription of ribosomal DNA (rDNA) takes place. During mitotic division, the nucleolus passes through different processes that inactivate rDNA transcription; in meiosis, its reassembly takes place during telophase II. The objective of this study was to identify the activity patterns and localization of nucleolar organizer regions (NORs) during meiotic division in fish species of the family Curimatidae. For this analysis, the meiotic division in five curimatid species was studied using silver nitrate impregnation, fluorescent in situ hybridization (FISH), and base-specific fluorochrome staining...
February 2014: Genome Génome / Conseil National de Recherches Canada
Naiara Pereira Araújo, Ana Carolina Loss, Dirceu A Cordeiro-Junior, Kátia Regina da Silva, Yuri L R Leite, Marta Svartman
Phyllomys (Echimyidae, Rodentia) is a genus of Neotropical rodents with available cytogenetic data restricted to six out of 13 species, mainly based on simple staining methods, without detailed analyses. In this work, we present new karyotypes for Phyllomys lamarum (diploid number 2n = 56, fundamental number or number of autosomal arms FN = 102) and Phyllomys sp. (2n = 74, FN = 140) from the state of Minas Gerais, southeastern Brazil. We provide the first GTG- and CBG-banding patterns, silver-staining of the nucleolar organizer regions (Ag-NORs), and fluorescence in situ hybridization (FISH) with telomeric and 45S rDNA probes of Phyllomys...
January 2014: Genome Génome / Conseil National de Recherches Canada
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