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Ana nucleolar

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https://www.readbyqxmd.com/read/27792899/functional-alterations-of-root-meristematic-cells-of-arabidopsis-thaliana-induced-by-a-simulated-microgravity-environment
#1
Elodie Boucheron-Dubuisson, Ana I Manzano, Isabel Le Disquet, Isabel Matía, Julio Sáez-Vasquez, Jack J W A van Loon, Raúl Herranz, Eugénie Carnero-Diaz, F Javier Medina
Environmental gravity modulates plant growth and development, and these processes are influenced by the balance between cell proliferation and differentiation in meristems. Meristematic cells are characterized by the coordination between cell proliferation and cell growth, that is, by the accurate regulation of cell cycle progression and the optimal production of biomass for the viability of daughter cells after division. Thus, cell growth is correlated with the rate of ribosome biogenesis and protein synthesis...
October 19, 2016: Journal of Plant Physiology
https://www.readbyqxmd.com/read/27615849/a-new-species-of-the-scinax-catharinae-group-anura-hylidae-from-southeastern-brazil
#2
Ana Carolina C Lourenço, Juliana Zina, Gaucilene F Catroli, Sanae Kasahara, Julian Faivovich, Célio F B Haddad
We describe a new species of the Scinax catharinae group from coastal forest in the State of São Paulo, Brazil. We also describe the karyotype and the advertisement call of the new species, which is composed by a multipulsed note. The chromosome morphology of the new species shows the nucleolar organizer region (NOR) on Pair 6, a putative synapomorphy previously inferred for the S. catharinae clade. We report, for the first time, the presence of mental glands in a species of the genus Scinax. Additionally, we provide a brief discussion about the occurrence of glandular tissue on the mental and inguinal regions of the species of the S...
2016: Zootaxa
https://www.readbyqxmd.com/read/27456346/chromosomal-characterization-in-two-species-of-an-astyanax-bimaculatus-complex-characidae-characiformes-using-different-techniques-of-chromosome-banding
#3
Laura L L Da Silva, Angélica R Dos Santos, Lucia Giuliano-Caetano, Ana L Dias
Astyanax has been the subject of extensive cytogenetic studies due to its wide karyotypic diversity. This genus comprises species complexes, namely groups of fish of difficult morphological differentiation, such as the bimaculatus complex, which includes the characids with a rounded humeral spot. Thence, the present study proposed to accomplish a cytogenetic characterization of two species of this complex: A. asuncionensis and A. altiparanae, aiming to find chromosomal markers that differentiate these species, as well as achieve a better understanding of the karyotype evolution in the genus...
August 2016: Cytotechnology
https://www.readbyqxmd.com/read/27395414/recognition-of-the-dense-fine-speckled-dfs-pattern-remains-challenging-results-from-an-international-internet-based-survey
#4
Chelsea Bentow, Marvin J Fritzler, Eckart Mummert, Michael Mahler
PURPOSE: The dense fine speckled (DFS) pattern as detected by indirect immunofluorescence (IIF) on HEp-2 cells has been associated with several inflammatory diseases but is most commonly observed in individuals that do not have an antinuclear antibody (ANA)-associated rheumatic disease and even in apparently healthy individuals. Consequently, the accurate identification and correct reporting of this IIF pattern is of utmost importance and accordingly has been recognized by several international study groups for the detection of ANA...
December 2016: Auto- Immunity Highlights
https://www.readbyqxmd.com/read/27311277/-evaluation-of-a-computer-aided-microscope-system-and-its-anti-nuclear-antibody-test-kit-for-indirect-immunofluorescence-assay
#5
Nobuhide Hayashi, Jun Saegusa, Kenichi Uto, Chinami Oyabu, Toshiharu Saito, Itsuko Sato, Seiji Kawano, Shunichi Kumagai
Antinuclear antibody (ANA) testing is indispensable for diagnosing and understanding clinical conditions of autoimmune diseases. The indirect immunofluorescence assay (IFA) is the gold standard for ANA screening, and it can detect more than 100 different antibodies, such as anti-PCNA as well as anti-cytoplasmic antibodies. However, complicated procedures of conventional IFA and visual interpretation require highly skilled laboratory staff. This study evaluates the capability, characteristics, and applicability of the recently developed ANA detection system (EUROPattern Cosmic IFA System, EPA) using HEp20-10 cells and the automated pattern recognition microscope...
February 2016: Rinsho Byori. the Japanese Journal of Clinical Pathology
https://www.readbyqxmd.com/read/27252267/development-and-multi-center-evaluation-of-a-novel-immunoadsorption-method-for-anti-dfs70-antibodies
#6
C Bentow, R Rosenblum, P Correia, E Karayev, D Karayev, D Williams, J Kulczycka, M J Fritzler, M Mahler
OBJECTIVE: Antinuclear antibodies (ANA) represent a hallmark in the diagnosis of ANA-associated rheumatic diseases (AARD). However, anti-DFS70 antibodies are present in a higher portion of the healthy individuals (HI) than in patients with AARD. Consequently, we developed a novel, highly specific indirect immunofluorescence (IIF) method that blocks anti-DFS70 antibodies from binding to HEp-2 cells and to evaluate the method in a multi-center study. METHODS: A total of 18 samples from systemic lupus erythematosus patients (SLE, n = 7) and HI (n = 11) were used for the initial development of the immunoadsorption method...
July 2016: Lupus
https://www.readbyqxmd.com/read/27173106/serum-antinuclear-antibody-in-adult-thais
#7
Chanwit Prapinjumrune, Chalakorn Prucktrakul, Trakarn Sooktonglarng, Kobkan Thongprasom
OBJECTIVE: This study investigated the presence of antinuclear antibody (ANA) in older Thais compared with middle-age and younger participants. BACKGROUND: Antinuclear antibody represents the first step in the diagnostic testing for lupus erythematosus (LE) and other autoimmune diseases. Due to the lack of reference ANA levels in older, middle-age and younger Thais healthy participants, this study will be useful for determining the proper diagnostic and treatment criteria...
May 12, 2016: Gerodontology
https://www.readbyqxmd.com/read/26899292/ameloblastic-neoplasia-spectrum-a-cross-sectional-study-of-mmps-expression-and-proliferative-activity
#8
Alessandra Dutra da Silva, Thaíse Gomes E Nóbrega, Annemarie Warstat Saudades, Maria Inês Otero, Chris Krebs Danilevicz, Alessandra Selinger Magnusson, Ana Luisa Saraiva Homem de Carvalho, Pantelis Varvaki Rados, Vinicius Coelho Carrard, Fernanda Visioli, Manoel Sant'Ana Filho
OBJECTIVE: To compare the proliferation and the expression of matrix metalloproteinases (MMPs; MMP-2 and MMP-9) in solid and unicystic ameloblastomas with ameloblastic carcinomas. STUDY DESIGN: Five cases of ameloblastic carcinoma (AC), 18 cases of solid ameloblastoma (SA), and seven of unicystic ameloblastoma (UA) were selected. The immunohistochemical expression of MMPs was assessed by the percentage of positive tumor cells and stained stroma. The mean argyrophilic nucleolar organizer region (AgNOR) and the percentage of cells with more than one AgNOR per nucleus were evaluated...
April 2016: Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
https://www.readbyqxmd.com/read/26861283/use-of-repetitive-sequences-for-molecular-and-cytogenetic-characterization-of-avena-species-from-portugal
#9
Diana Tomás, Joana Rodrigues, Ana Varela, Maria Manuela Veloso, Wanda Viegas, Manuela Silva
Genomic diversity of Portuguese accessions of Avena species--diploid A. strigosa and hexaploids A. sativa and A. sterilis--was evaluated through molecular and cytological analysis of 45S rDNA, and other repetitive sequences previously studied in cereal species--rye subtelomeric sequence (pSc200) and cereal centromeric sequence (CCS1). Additionally, retrotransposons and microsatellites targeting methodologies--IRAP (inter-retrotransposon amplified polymorphism) and REMAP (retrotransposon-microsatellite amplified polymorphism)--were performed...
2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/26846693/serological-profile-and-clinical-features-of-nucleolar-antinuclear-pattern-in-patients-with-systemic-lupus-erythematosus-from-southwestern-spain
#10
C M Cabrera, E Fernández-Grande, J M Urra
Nucleolar staining of antinuclear antibodies (ANAs) is not exclusive to patients suffering systemic sclerosis (SSc) since it can occur in other autoimmune diseases, such as systemic lupus erythematosus (SLE). The nucleolar ANA pattern presents a low incidence in patients with SLE, with less than 9% reported in some studies. The significance of nucleolar staining and antinucleolar antibodies (ANoA) in SLE is still unknown, as is its association with clinical manifestations. To address these issues, a case-control study was carried out...
August 2016: Lupus
https://www.readbyqxmd.com/read/26289629/design-of-a-computer-assisted-system-to-automatically-detect-cell-types-using-ana-iif-images-for-the-diagnosis-of-autoimmune-diseases
#11
Chung-Chuan Cheng, Chun-Feng Lu, Tsu-Yi Hsieh, Yaw-Jen Lin, Jin-Shiuh Taur, Yung-Fu Chen
Indirect immunofluorescence technique applied on HEp-2 cell substrates provides the major screening method to detect ANA patterns in the diagnosis of autoimmune diseases. Currently, the ANA patterns are mostly inspected by experienced physicians to identify abnormal cell patterns. The objective of this study is to design a computer-assisted system to automatically detect cell patterns of IIF images for the diagnosis of autoimmune diseases in the clinical setting. The system simulates the functions of modern flow cytometer and provides the diagnostic reports generated by the system to the technicians and physicians through the radar graphs, box-plots, and tables...
October 2015: Journal of Medical Systems
https://www.readbyqxmd.com/read/26248216/exposure-of-human-prostaspheres-to-bisphenol-a-epigenetically-regulates-snord-family-noncoding-rnas-via-histone-modification
#12
Shuk-Mei Ho, Ana Cheong, Hung-Ming Lam, Wen-Yang Hu, Guang-Bin Shi, Xuegong Zhu, Jing Chen, Xiang Zhang, Mario Medvedovic, Yuet-Kin Leung, Gail S Prins
Bisphenol A (BPA) is a ubiquitous endocrine disruptor exerting lifelong effects on gene expression in rodent prostate cancer (PCa) models. Here, we aimed to determine whether epigenetic events mediating the action of BPA on human prostaspheres enriched in epithelial stem-like/progenitor cells is linked to PCa. We performed genome-wide transcriptome and methylome analyses to identify changes in prostaspheres treated with BPA (10 nM, 200 nM, and 1000 nM) or estradiol-17β (E2) (0.1 nM) for 7 days and validated changes in expression, methylation, and histone marks in parallel-treated prostaspheres...
November 2015: Endocrinology
https://www.readbyqxmd.com/read/26210782/survival-in-systemic-sclerosis-pulmonary-arterial-hypertension-by-serum-autoantibody-status-in-the-pulmonary-hypertension-assessment-and-recognition-of-outcomes-in-scleroderma-pharos-registry
#13
Monique Hinchcliff, Saira Khanna, Vivien M Hsu, Jungwha Lee, Orit Almagor, Rowland W Chang, Virginia Steen, Lorinda Chung
OBJECTIVE: To determine the association between serum autoantibodies and survival in patients with incident systemic sclerosis (SSc)-pulmonary arterial hypertension (PAH) enrolled in the Pulmonary Hypertension Assessment and Recognition of Outcomes in Scleroderma (PHAROS) Registry. METHODS: Patients with definite PAH diagnosed by right heart catheterization within 6 months of registry enrollment were studied. Serum autoantibodies were assayed at each participating institution's clinical laboratory...
December 2015: Seminars in Arthritis and Rheumatism
https://www.readbyqxmd.com/read/26139346/arabidopsis-mas2-an-essential-gene-that-encodes-a-homolog-of-animal-nf-%C3%AE%C2%BA-b-activating-protein-is-involved-in-45s-ribosomal-dna-silencing
#14
Ana Belén Sánchez-García, Verónica Aguilera, Rosa Micol-Ponce, Sara Jover-Gil, María Rosa Ponce
Ribosome biogenesis requires stoichiometric amounts of ribosomal proteins and rRNAs. Synthesis of rRNAs consumes most of the transcriptional activity of eukaryotic cells, but its regulation remains largely unclear in plants. We conducted a screen for ethyl methanesulfonate-induced suppressors of Arabidopsis thaliana ago1-52, a hypomorphic allele of AGO1 (ARGONAUTE1), a key gene in microRNA pathways. We identified nine extragenic suppressors as alleles of MAS2 (MORPHOLOGY OF AGO1-52 SUPPRESSED2). Positional cloning showed that MAS2 encodes the putative ortholog of NKAP (NF-κ B activating protein), a conserved eukaryotic protein involved in transcriptional repression and splicing in animals...
July 2015: Plant Cell
https://www.readbyqxmd.com/read/26031162/-the-automated-analysis-of-anti-nuclear-antibodies-using-technique-of-indirect-reaction-of-immunofluorescence-with-application-of-hep-2-cells
#15
E N Aleksandrova, J G Verijnikova, A A Novikov, A A Baranov, N E Abaitova, N A Lapkina, D Roggenbuk, E L Nasonov
The identification of antinuclear antibodies in blood serum based on indirect reaction of immunofluorescence using cells of line HEp-2 (IRIF HEp-2)--a "golden standard" and key screening technique of laboratory diagnostic of autoimmune rheumatic diseases. The automated systems of interpretation of samples offluorescence promote standardization and increase effectiveness of detection of content of antinuclear antibodies with IRIF HEp-2 technique. The study was organized to comparatively analyze automated and visual interpretation of results of IRIF HEp-2 in detection of content antinuclear antibodies in patients with rheumatic diseases...
March 2015: Klinicheskaia Laboratornaia Diagnostika
https://www.readbyqxmd.com/read/25962196/a-study-on-pulmonary-complications-of-systemic-sclerosis-in-eastern-india
#16
Anirban Ghosh, Avinandan Banerjee, Amlan Kanti Biswas
AIM: This study was undertaken to find out the characteristics of clinical, radiological and functional changes affecting the respiratory system in patients with systemic sclerosis (SSc) from eastern India, and the association of these characteristics with pulmonary hypertension. METHODS: This was a cross-sectional, observational study involving 46 patients. Other than the routine tests, anti-nuclear antibody (ANA), spirometry, diffusing capacity of lung for carbon monoxide (DLCO) measurement, chest radiograph, high-resolution computed tomography (HRCT) of thorax, 6-minute walk test and echocardiography were done...
October 2014: Indian Journal of Chest Diseases & Allied Sciences
https://www.readbyqxmd.com/read/25570930/analysis-of-hep-2-images-using-md-lbp-and-mad-bagging
#17
Gerald Schaefer, Niraj P Doshi, Shao Ying Zhu, Qinghua Hu
Indirect immunofluorescence imaging is employed to identify antinuclear antibodies in HEp-2 cells which founds the basis for diagnosing autoimmune diseases and other important pathological conditions involving the immune system. Six categories of HEp-2 cells are generally considered, namely homogeneous, fine speckled, coarse speckled, nucleolar, cyto-plasmic, and centromere cells. Typically, this categorisation is performed manually by an expert and is hence both time consuming and subjective. In this paper, we present a method for automatically classifiying HEp-2 cells using texture information in conjunction with a suitable classification system...
2014: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/25533500/relationship-between-certain-t-helper-cytokines-and-ana-staining-who-is-the-helper
#18
Jinhui Hu, Yuyan Zhang, Xinghui Liu, Li Wei, Denghai Zhang
OBJECTIVE: This study investigated some T helper cytokines' association with different ANA positive sera. METHODS: Sera of 53 ANA-positive subjects (11 were stained of homogeneous pattern, 12 of speckled particle pattern, 9 of nucleolar pattern, 15 of centromere pattern, and 6 of the peripheral nuclear type) were collected and analyzed by ELISA for related cytokines and concentrations were compared with each other and 16 healthy donors. RESULTS: Investigated cytokines including IL-12(p70) and IFN-γ varied differently in the ANA sera...
February 2015: International Immunopharmacology
https://www.readbyqxmd.com/read/25495140/cryptic-speciation-within-the-neotropical-cichlid-geophagus-brasiliensis-quoy-gaimard-1824-teleostei-cichlidae-a-new-paradigm-in-karyotypical-and-molecular-evolution
#19
Ana Paula Alves-Silva, Jorge Abdala Dergam
The family Cichlidae is one of the most species-rich taxa in the Neotropics. However, the factors that determine these high levels of biodiversity remain unexplored. We have analyzed the morphological, cytogenetic, and molecular data from 62 specimens of a widespread cichlid, Geophagus brasiliensis, from three adjacent basins in southeastern Brazil. Morphological analyses did not show differences among specimens. The cytogenetic data indicate the occurrence of multiple nucleolar organizer regions and four sympatric karyotypes that differ in the first pair of chromosome morphology, in the Doce River Basin; whereas the karyotype from the Paraíba do Sul Basin is widely divergent...
February 2015: Zebrafish
https://www.readbyqxmd.com/read/25477391/the-association-of-late-acting-snornps-with-human-pre-ribosomal-complexes-requires-the-rna-helicase-ddx21
#20
Katherine E Sloan, Matthias S Leisegang, Carmen Doebele, Ana S Ramírez, Stefan Simm, Charlotta Safferthal, Jens Kretschmer, Tobias Schorge, Stavroula Markoutsa, Sara Haag, Michael Karas, Ingo Ebersberger, Enrico Schleiff, Nicholas J Watkins, Markus T Bohnsack
Translation fidelity and efficiency require multiple ribosomal (r)RNA modifications that are mostly mediated by small nucleolar (sno)RNPs during ribosome production. Overlapping basepairing of snoRNAs with pre-rRNAs often necessitates sequential and efficient association and dissociation of the snoRNPs, however, how such hierarchy is established has remained unknown so far. Here, we identify several late-acting snoRNAs that bind pre-40S particles in human cells and show that their association and function in pre-40S complexes is regulated by the RNA helicase DDX21...
January 2015: Nucleic Acids Research
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