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Ana nucleolar

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https://www.readbyqxmd.com/read/28768567/in-vitro-growth-and-development-of-isolated-secondary-follicles-from-vitrified-caprine-ovarian-cortex
#1
Érica S S Leal, Luis A Vieira, Naíza A R Sá, Gerlane M Silva, Franciele O Lunardi, Anna C A Ferreira, Cláudio C Campello, Benner G Alves, Francielli W S Cibin, Johan Smitz, José R Figueiredo, Ana P R Rodrigues
The aim of this study was to evaluate the viability, antrum formation and in vitro development of isolated secondary follicles from vitrified caprine ovarian cortex in a medium previously established for fresh isolated secondary follicles, in the absence (α-minimum essential medium (α-MEM+) alone) or presence of FSH and vascular endothelial growth factor (VEGF; α-MEM++FSH+VEGF). Ovarian fragments were distributed among five treatments (T1 to T5): fresh follicles were fixed immediately (T1), follicles from fresh tissue were cultured in vitro in α-MEM+ (T2) or α-MEM++FSH+VEGF (T3) and follicles from vitrified tissue were cultured in vitro in α-MEM+ (T4) or α-MEM++FSH+VEGF (T5)...
August 3, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28609254/chromosomal-diversity-of-three-species-of-hypostomus-lac%C3%A3-p%C3%A3-de-1803-siluriformes-loricariidae-from-the-paran%C3%A3-river-basin-brazil-a-species-complex-in-hypostomus-ancistroides-reinforced-by-a-zz-zw-sex-chromosome-system
#2
Marcia Cristina de Souza Lara Kamei, Lucas Baumgärtner, Suzana Paiva, Cláudio Henrique Zawadzki, Isabel Cristina Martins-Santos, Ana Luiza de Brito Portela-Castro
Hypostomus shows wide morphological patterns, corroborated by great chromosomal diversity that has suggested the existence of new species, especially from small tributaries. Cytogenetic analysis has contributed to estimate a cryptic diversity providing important data for taxonomic and evolutionary studies. Cytogenetic techniques were carried out on species from a small tributary of Ivaí River, Keller River (upper Paraná River basin): Hypostomus aff. ancistroides, Hypostomus topavae, and Hypostomus aff. hermanni that presented 2n = 68, 80, and 72 chromosomes, respectively...
August 2017: Zebrafish
https://www.readbyqxmd.com/read/28467309/automated-antinuclear-immunofluorescence-antibody-analysis-is-a-reliable-approach-in-routine-clinical-laboratories
#3
Bing Zheng, Enling Li, Haoming Zhu, Jingbo Lu, Xinming Shi, Jie Zhang, Min Li
BACKGROUND: Indirect immunofluorescence (IIF) assays are recommended as the gold standard method for the detection of antinuclear antibodies (ANAs). This study aimed to investigate the reliability of an automated system. METHODS: We compared 3745 serum samples using NOVA View archived images with manual analysis via microscopy. A custom cutoff value was established to distinguish ANA titers and was validated in two clinical laboratories. The automatic ANA pattern recognition system was evaluated, and all ANA-positive sera were subjected to two commercial ANA IIF kits to compare the consistency of the pattern interpretation results...
May 3, 2017: Clinical Chemistry and Laboratory Medicine: CCLM
https://www.readbyqxmd.com/read/28448962/progressive-changes-in-non-coding-rna-profile-in-leucocytes-with-age
#4
Maider Muñoz-Culla, Haritz Irizar, Ana Gorostidi, Ainhoa Alberro, Iñaki Osorio-Querejeta, Javier Ruiz-Martínez, Javier Olascoaga, Adolfo López de Munain, David Otaegui
It has been observed that immune cell deterioration occurs in the elderly, as well as a chronic low-grade inflammation called inflammaging. These cellular changes must be driven by numerous changes in gene expression and in fact, both protein-coding and non-coding RNA expression alterations have been observed in peripheral blood mononuclear cells from elder people. In the present work we have studied the expression of small non-coding RNA (microRNA and small nucleolar RNA -snoRNA-) from healthy individuals from 24 to 79 years old...
April 2017: Aging
https://www.readbyqxmd.com/read/27994142/regulatory-module-involving-fgf13-mir-504-and-p53-regulates-ribosomal-biogenesis-and-supports-cancer-cell-survival
#5
Débora R Bublik, Slađana Bursać, Michal Sheffer, Ines Oršolić, Tali Shalit, Ohad Tarcic, Eran Kotler, Odelia Mouhadeb, Yonit Hoffman, Gilad Fuchs, Yishai Levin, Siniša Volarević, Moshe Oren
The microRNA miR-504 targets TP53 mRNA encoding the p53 tumor suppressor. miR-504 resides within the fibroblast growth factor 13 (FGF13) gene, which is overexpressed in various cancers. We report that the FGF13 locus, comprising FGF13 and miR-504, is transcriptionally repressed by p53, defining an additional negative feedback loop in the p53 network. Furthermore, we show that FGF13 1A is a nucleolar protein that represses ribosomal RNA transcription and attenuates protein synthesis. Importantly, in cancer cells expressing high levels of FGF13, the depletion of FGF13 elicits increased proteostasis stress, associated with the accumulation of reactive oxygen species and apoptosis...
January 24, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/27979657/undifferentiated-connective-tissue-disease-and-interstitial-lung-disease-trying-to-define-patterns
#6
María Laura Alberti, Francisco Paulin, Heidegger Mateos Toledo, Martín Eduardo Fernández, Fabián Matías Caro, Jorge Rojas-Serrano, Mayra Edith Mejía
OBJECTIVES: To identify clinical or immunological features in patients with undifferentiated connective tissue disease (UCTD) associated interstitial lung disease (ILD), in order to group them and recognize different functional and high resolution computed tomography (HRCT) behavior. METHODS: Retrospective cohort study. Patients meeting Kinder criteria for UCTD were included. We defined the following predictive variables: 'highly specific' connective tissue disease (CTD) manifestations (Raynaud's phenomenon, dry eyes or arthritis), high antinuclear antibody (ANA) titer (above 1: 320), and 'specific' ANA staining patterns (centromere, cytoplasmic and nucleolar patterns)...
December 12, 2016: Reumatología Clinica
https://www.readbyqxmd.com/read/27916273/identification-of-agnors-and-cytopathological-changes-in-oral-lichen-planus-lesions
#7
Stefânia Jeronimo Ferreira, Maria Ângela Naval Machado, Antônio Adilson Soares de Lima, Aline Cristina Batista Rodrigues Johann, Ana Maria Trindade Grégio, Luciana Reis Azevedo-Alanis
OBJECTIVE: To evaluate cytopathological changes in epithelial cells of the oral mucosa of patients with oral lichen planus (OLP) compared with patients without OLP. SUBJECTS AND METHODS: Swabs were collected from the oral mucosa of 20 patients with OLP (case group) and 20 patients without OLP (control group) using liquid-based cytology. After Papanicolaou staining, the smears were characterized based on Papanicolaou classification and degree of maturation. Nuclear area (NA) measurements, cytoplasmic area (CA) measurements, and the NA/CA ratio were determined from 50 epithelial cells per slide...
January 2017: Acta Histochemica
https://www.readbyqxmd.com/read/27792899/functional-alterations-of-root-meristematic-cells-of-arabidopsis-thaliana-induced-by-a-simulated-microgravity-environment
#8
Elodie Boucheron-Dubuisson, Ana I Manzano, Isabel Le Disquet, Isabel Matía, Julio Sáez-Vasquez, Jack J W A van Loon, Raúl Herranz, Eugénie Carnero-Diaz, F Javier Medina
Environmental gravity modulates plant growth and development, and these processes are influenced by the balance between cell proliferation and differentiation in meristems. Meristematic cells are characterized by the coordination between cell proliferation and cell growth, that is, by the accurate regulation of cell cycle progression and the optimal production of biomass for the viability of daughter cells after division. Thus, cell growth is correlated with the rate of ribosome biogenesis and protein synthesis...
December 1, 2016: Journal of Plant Physiology
https://www.readbyqxmd.com/read/27615849/a-new-species-of-the-scinax-catharinae-group-anura-hylidae-from-southeastern-brazil
#9
Ana Carolina C Lourenço, Juliana Zina, Gaucilene F Catroli, Sanae Kasahara, Julian Faivovich, Célio F B Haddad
We describe a new species of the Scinax catharinae group from coastal forest in the State of São Paulo, Brazil. We also describe the karyotype and the advertisement call of the new species, which is composed by a multipulsed note. The chromosome morphology of the new species shows the nucleolar organizer region (NOR) on Pair 6, a putative synapomorphy previously inferred for the S. catharinae clade. We report, for the first time, the presence of mental glands in a species of the genus Scinax. Additionally, we provide a brief discussion about the occurrence of glandular tissue on the mental and inguinal regions of the species of the S...
August 23, 2016: Zootaxa
https://www.readbyqxmd.com/read/27456346/chromosomal-characterization-in-two-species-of-an-astyanax-bimaculatus-complex-characidae-characiformes-using-different-techniques-of-chromosome-banding
#10
Laura L L Da Silva, Angélica R Dos Santos, Lucia Giuliano-Caetano, Ana L Dias
Astyanax has been the subject of extensive cytogenetic studies due to its wide karyotypic diversity. This genus comprises species complexes, namely groups of fish of difficult morphological differentiation, such as the bimaculatus complex, which includes the characids with a rounded humeral spot. Thence, the present study proposed to accomplish a cytogenetic characterization of two species of this complex: A. asuncionensis and A. altiparanae, aiming to find chromosomal markers that differentiate these species, as well as achieve a better understanding of the karyotype evolution in the genus...
August 2016: Cytotechnology
https://www.readbyqxmd.com/read/27395414/recognition-of-the-dense-fine-speckled-dfs-pattern-remains-challenging-results-from-an-international-internet-based-survey
#11
Chelsea Bentow, Marvin J Fritzler, Eckart Mummert, Michael Mahler
PURPOSE: The dense fine speckled (DFS) pattern as detected by indirect immunofluorescence (IIF) on HEp-2 cells has been associated with several inflammatory diseases but is most commonly observed in individuals that do not have an antinuclear antibody (ANA)-associated rheumatic disease and even in apparently healthy individuals. Consequently, the accurate identification and correct reporting of this IIF pattern is of utmost importance and accordingly has been recognized by several international study groups for the detection of ANA...
December 2016: Auto- Immunity Highlights
https://www.readbyqxmd.com/read/27311277/-evaluation-of-a-computer-aided-microscope-system-and-its-anti-nuclear-antibody-test-kit-for-indirect-immunofluorescence-assay
#12
Nobuhide Hayashi, Jun Saegusa, Kenichi Uto, Chinami Oyabu, Toshiharu Saito, Itsuko Sato, Seiji Kawano, Shunichi Kumagai
Antinuclear antibody (ANA) testing is indispensable for diagnosing and understanding clinical conditions of autoimmune diseases. The indirect immunofluorescence assay (IFA) is the gold standard for ANA screening, and it can detect more than 100 different antibodies, such as anti-PCNA as well as anti-cytoplasmic antibodies. However, complicated procedures of conventional IFA and visual interpretation require highly skilled laboratory staff. This study evaluates the capability, characteristics, and applicability of the recently developed ANA detection system (EUROPattern Cosmic IFA System, EPA) using HEp20-10 cells and the automated pattern recognition microscope...
February 2016: Rinsho Byori. the Japanese Journal of Clinical Pathology
https://www.readbyqxmd.com/read/27252267/development-and-multi-center-evaluation-of-a-novel-immunoadsorption-method-for-anti-dfs70-antibodies
#13
MULTICENTER STUDY
C Bentow, R Rosenblum, P Correia, E Karayev, D Karayev, D Williams, J Kulczycka, M J Fritzler, M Mahler
OBJECTIVE: Antinuclear antibodies (ANA) represent a hallmark in the diagnosis of ANA-associated rheumatic diseases (AARD). However, anti-DFS70 antibodies are present in a higher portion of the healthy individuals (HI) than in patients with AARD. Consequently, we developed a novel, highly specific indirect immunofluorescence (IIF) method that blocks anti-DFS70 antibodies from binding to HEp-2 cells and to evaluate the method in a multi-center study. METHODS: A total of 18 samples from systemic lupus erythematosus patients (SLE, n = 7) and HI (n = 11) were used for the initial development of the immunoadsorption method...
July 2016: Lupus
https://www.readbyqxmd.com/read/27173106/serum-antinuclear-antibody-in-adult-thais
#14
Chanwit Prapinjumrune, Chalakorn Prucktrakul, Trakarn Sooktonglarng, Kobkan Thongprasom
OBJECTIVE: This study investigated the presence of antinuclear antibody (ANA) in older Thais compared with middle-age and younger participants. BACKGROUND: Antinuclear antibody represents the first step in the diagnostic testing for lupus erythematosus (LE) and other autoimmune diseases. Due to the lack of reference ANA levels in older, middle-age and younger Thais healthy participants, this study will be useful for determining the proper diagnostic and treatment criteria...
May 12, 2016: Gerodontology
https://www.readbyqxmd.com/read/26899292/ameloblastic-neoplasia-spectrum-a-cross-sectional-study-of-mmps-expression-and-proliferative-activity
#15
Alessandra Dutra da Silva, Thaíse Gomes E Nóbrega, Annemarie Warstat Saudades, Maria Inês Otero, Chris Krebs Danilevicz, Alessandra Selinger Magnusson, Ana Luisa Saraiva Homem de Carvalho, Pantelis Varvaki Rados, Vinicius Coelho Carrard, Fernanda Visioli, Manoel Sant'Ana Filho
OBJECTIVE: To compare the proliferation and the expression of matrix metalloproteinases (MMPs; MMP-2 and MMP-9) in solid and unicystic ameloblastomas with ameloblastic carcinomas. STUDY DESIGN: Five cases of ameloblastic carcinoma (AC), 18 cases of solid ameloblastoma (SA), and seven of unicystic ameloblastoma (UA) were selected. The immunohistochemical expression of MMPs was assessed by the percentage of positive tumor cells and stained stroma. The mean argyrophilic nucleolar organizer region (AgNOR) and the percentage of cells with more than one AgNOR per nucleus were evaluated...
April 2016: Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
https://www.readbyqxmd.com/read/26861283/use-of-repetitive-sequences-for-molecular-and-cytogenetic-characterization-of-avena-species-from-portugal
#16
Diana Tomás, Joana Rodrigues, Ana Varela, Maria Manuela Veloso, Wanda Viegas, Manuela Silva
Genomic diversity of Portuguese accessions of Avena species--diploid A. strigosa and hexaploids A. sativa and A. sterilis--was evaluated through molecular and cytological analysis of 45S rDNA, and other repetitive sequences previously studied in cereal species--rye subtelomeric sequence (pSc200) and cereal centromeric sequence (CCS1). Additionally, retrotransposons and microsatellites targeting methodologies--IRAP (inter-retrotransposon amplified polymorphism) and REMAP (retrotransposon-microsatellite amplified polymorphism)--were performed...
February 4, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/26846693/serological-profile-and-clinical-features-of-nucleolar-antinuclear-pattern-in-patients-with-systemic-lupus-erythematosus-from-southwestern-spain
#17
C M Cabrera, E Fernández-Grande, J M Urra
Nucleolar staining of antinuclear antibodies (ANAs) is not exclusive to patients suffering systemic sclerosis (SSc) since it can occur in other autoimmune diseases, such as systemic lupus erythematosus (SLE). The nucleolar ANA pattern presents a low incidence in patients with SLE, with less than 9% reported in some studies. The significance of nucleolar staining and antinucleolar antibodies (ANoA) in SLE is still unknown, as is its association with clinical manifestations. To address these issues, a case-control study was carried out...
August 2016: Lupus
https://www.readbyqxmd.com/read/26289629/design-of-a-computer-assisted-system-to-automatically-detect-cell-types-using-ana-iif-images-for-the-diagnosis-of-autoimmune-diseases
#18
Chung-Chuan Cheng, Chun-Feng Lu, Tsu-Yi Hsieh, Yaw-Jen Lin, Jin-Shiuh Taur, Yung-Fu Chen
Indirect immunofluorescence technique applied on HEp-2 cell substrates provides the major screening method to detect ANA patterns in the diagnosis of autoimmune diseases. Currently, the ANA patterns are mostly inspected by experienced physicians to identify abnormal cell patterns. The objective of this study is to design a computer-assisted system to automatically detect cell patterns of IIF images for the diagnosis of autoimmune diseases in the clinical setting. The system simulates the functions of modern flow cytometer and provides the diagnostic reports generated by the system to the technicians and physicians through the radar graphs, box-plots, and tables...
October 2015: Journal of Medical Systems
https://www.readbyqxmd.com/read/26248216/exposure-of-human-prostaspheres-to-bisphenol-a-epigenetically-regulates-snord-family-noncoding-rnas-via-histone-modification
#19
Shuk-Mei Ho, Ana Cheong, Hung-Ming Lam, Wen-Yang Hu, Guang-Bin Shi, Xuegong Zhu, Jing Chen, Xiang Zhang, Mario Medvedovic, Yuet-Kin Leung, Gail S Prins
Bisphenol A (BPA) is a ubiquitous endocrine disruptor exerting lifelong effects on gene expression in rodent prostate cancer (PCa) models. Here, we aimed to determine whether epigenetic events mediating the action of BPA on human prostaspheres enriched in epithelial stem-like/progenitor cells is linked to PCa. We performed genome-wide transcriptome and methylome analyses to identify changes in prostaspheres treated with BPA (10 nM, 200 nM, and 1000 nM) or estradiol-17β (E2) (0.1 nM) for 7 days and validated changes in expression, methylation, and histone marks in parallel-treated prostaspheres...
November 2015: Endocrinology
https://www.readbyqxmd.com/read/26210782/survival-in-systemic-sclerosis-pulmonary-arterial-hypertension-by-serum-autoantibody-status-in-the-pulmonary-hypertension-assessment-and-recognition-of-outcomes-in-scleroderma-pharos-registry
#20
Monique Hinchcliff, Saira Khanna, Vivien M Hsu, Jungwha Lee, Orit Almagor, Rowland W Chang, Virginia Steen, Lorinda Chung
OBJECTIVE: To determine the association between serum autoantibodies and survival in patients with incident systemic sclerosis (SSc)-pulmonary arterial hypertension (PAH) enrolled in the Pulmonary Hypertension Assessment and Recognition of Outcomes in Scleroderma (PHAROS) Registry. METHODS: Patients with definite PAH diagnosed by right heart catheterization within 6 months of registry enrollment were studied. Serum autoantibodies were assayed at each participating institution's clinical laboratory...
December 2015: Seminars in Arthritis and Rheumatism
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