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https://www.readbyqxmd.com/read/29454005/a-quest-to-find-good-primers-for-gene-expression-analysis-of-candida-albicans-from-clinical-samples
#1
Gabriela C Alonso, Ana C Pavarina, Tábata V Sousa, Marlise I Klein
Biofilm production contributes to several human diseases, including oral candidiasis. Among the Candida species, Candida albicans is the most prevalent. The expression of virulence genes is implicated in the pathogenic potential of Candida biofilms. However, the evaluation of microbial gene expression from in vivo biofilm samples is not trivial, specifically, assessment via quantitative PCR (qPCR) can be a challenge because of several species present in clinical samples. Hence, the necessity of primers specificity...
February 14, 2018: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/29447925/dj-1-is-a-useful-biomarker-for-invasive-extrahepatic-cholangiocarcinoma
#2
Yukiko Tabata, Yoshitsugu Nakanishi, Kanako C Hatanaka, Yutaka Hatanaka, Takahiro Tsuchikawa, Keisuke Okamura, Takehiro Noji, Toshiaki Shichinohe, Yoshihiro Matsuno, Satoshi Hirano
We have previously reported that DJ-1 protein is up-regulated in cholangiocarcinoma compared with non-neoplastic epithelium of the bile duct in a study using liquid chromatography mass spectrometry-based proteomics. The aim of this study was to clarify whether DJ-1 expression offers a biomarker for patients with invasive extrahepatic cholangiocarcinoma (EHCC) who undergo surgical resection with curative intent. Positive immunohistochemical (IHC) staining of DJ-1 was significantly more frequent in the cytoplasm of 96 invasive EHCCs (n=28, 29...
February 12, 2018: Human Pathology
https://www.readbyqxmd.com/read/29440260/chromatin-remodeler-chd7-regulates-the-stem-cell-identity-of-human-neural-progenitors
#3
MuhChyi Chai, Tsukasa Sanosaka, Hironobu Okuno, Zhi Zhou, Ikuko Koya, Satoe Banno, Tomoko Andoh-Noda, Yoshikuni Tabata, Rieko Shimamura, Tetsutaro Hayashi, Masashi Ebisawa, Yohei Sasagawa, Itoshi Nikaido, Hideyuki Okano, Jun Kohyama
Multiple congenital disorders often present complex phenotypes, but how the mutation of individual genetic factors can lead to multiple defects remains poorly understood. In the present study, we used human neuroepithelial (NE) cells and CHARGE patient-derived cells as an in vitro model system to identify the function of chromodomain helicase DNA-binding 7 (CHD7) in NE-neural crest bifurcation, thus revealing an etiological link between the central nervous system (CNS) and craniofacial anomalies observed in CHARGE syndrome...
January 15, 2018: Genes & Development
https://www.readbyqxmd.com/read/29425339/cd28-co-stimulation-is-dispensable-for-the-steady-state-homeostasis-of-intestinal-regulatory-t-cells
#4
Ei Wakamatsu, Hiroki Omori, Yuki Tabata, Yuki Akieda, Shiho Watanabe, Shuhei Ogawa, Ryo Abe
It is well-established that CD28 co-stimulation is required for the development and the proliferation of thymus-derived regulatory T cells (tTregs). Meanwhile, the role of CD28 co-stimulation in the homeostasis of peripherally-derived Tregs (pTregs) remains unclear. To clarify this issue, we analyzed Tregs in small and large intestines (SI and LI), the principle sites of pTreg development. Interestingly, and different from in the thymus, Tregs were abundant in the intestines of CD28-/- mice, and most of them were phenotypically pTregs...
February 7, 2018: International Immunology
https://www.readbyqxmd.com/read/29423680/changes-in-upper-extremity-function-adl-and-hrqol-in-colorectal-cancer-patients-after-the-first-chemotherapy-cycle-with-oxaliplatin-a-prospective-single-center-observational-study
#5
Ami Tabata, Masashi Kanai, Takahiro Horimatsu, Tadao Tsuboyama, Kanae Matsushima, Toshihiro Kato
PURPOSE: Oxaliplatin, an important chemotherapeutic agent in colorectal cancer, causes chemotherapy-induced peripheral neuropathy (CIPN), for which prophylactic or therapeutic interventions are lacking. We aimed to investigate changes in upper extremities, activities of daily living (ADL), and health-related quality of life (HRQoL) parameters after the first chemotherapy cycle. METHODS: Thirty-eight colorectal cancer patients scheduled to receive the leucovorin, 5'-fluorouracil, oxaliplatin (FOLFOX) therapy or the capecitabine, oxaliplatin (CAPOX) therapy, participated...
February 8, 2018: Supportive Care in Cancer: Official Journal of the Multinational Association of Supportive Care in Cancer
https://www.readbyqxmd.com/read/29422016/malignancy-during-pregnancy-in-japan-an-exceptional-opportunity-for-early-diagnosis
#6
Masayuki Sekine, Yoshiyuki Kobayashi, Tsutomu Tabata, Tamotsu Sudo, Ryuichiro Nishimura, Koji Matsuo, Brendan H Grubbs, Takayuki Enomoto, Tomoaki Ikeda
BACKGROUND: Malignancy during pregnancy has become a significant cause of maternal death in developed countries, likely due to both an older pregnant population, and increases of cervical cancer in younger women. Our aim is to investigate the clinical aspects of malignancy during pregnancy in Japan and to use this information to identify opportunities for earlier detection and treatment. METHODS: We provided a questionnaire to 1508 secondary or tertiary care hospitals in Japan...
February 8, 2018: BMC Pregnancy and Childbirth
https://www.readbyqxmd.com/read/29415143/safety-and-durability-of-the-biodegradable-felt-in-aortic-surgery-a-propensity-score-matched-study
#7
Katsuhiro Hosoyama, Shunsuke Kawamoto, Koyu Watanabe, Yumi Sugawara, Yasuhiko Tabata, Masaya Yamamoto, Konosuke Sasaki, Koichi Tabayashi, Yoshikatsu Saiki
OBJECTIVES: Suture line disruption is a serious complication after aortic surgery. We previously reported in a canine model that basic fibroblast growth factor-incorporated biodegradable polyglycolic acid (PGA) felt prevented tissue derangement at the anastomotic site. This study sought to evaluate the safety and durability of this biodegradable felt. METHODS: Between January 2007 and December 2011, 67 patients who consented to undergo aortic surgery with the basic fibroblast growth factor-incorporated PGA felt were enrolled (Group P)...
February 5, 2018: European Journal of Cardio-thoracic Surgery
https://www.readbyqxmd.com/read/29409038/second-primary-cancer-in-survivors-of-locally-advanced-non-small-cell-lung-cancer-treated-with-concurrent-chemoradiation-followed-by-surgery
#8
Go Makimoto, Toshio Kubo, Isao Oze, Kadoaki Ohashi, Katsuyuki Hotta, Masahiro Tabata, Junichi Soh, Shinichi Toyooka, Kuniaki Katsui, Nagio Takigawa, Mitsune Tanimoto, Katsuyuki Kiura
The standard treatment for patients with locally advanced non-small-cell lung cancer (LA-NSCLC) is chemoradiotherapy (CRT), but surgical resection following induction CRT can extend overall survival in a select population. However, patients who survive longer are at risk of developing a second primary cancer (SPC). This is the first report to determine the incidence of SPC in survivors with LA-NSCLC after trimodal therapy. Between October 1997 and October 2013, 112 Stage III NSCLC patients underwent trimodal therapy in our hospital...
February 2, 2018: Japanese Journal of Clinical Oncology
https://www.readbyqxmd.com/read/29403328/4d-printing-of-polymeric-materials-for-tissue-and-organ-regeneration
#9
Shida Miao, Nathan Castro, Margaret Nowicki, Lang Xia, Haitao Cui, Xuan Zhou, Wei Zhu, Se-Jun Lee, Kausik Sarkar, Giovanni Vozzi, Yasuhiko Tabata, John Fisher, Lijie Grace Zhang
Four dimensional (4D) printing is an emerging technology with great capacity for fabricating complex, stimuli-responsive 3D structures, providing great potential for tissue and organ engineering applications. Although the 4D concept was first highlighted in 2013, extensive research has rapidly developed, along with more-in-depth understanding and assertions regarding the definition of 4D. In this review, we begin by establishing the criteria of 4D printing, followed by an extensive summary of state-of-the-art technological advances in the field...
December 2017: Materials Today
https://www.readbyqxmd.com/read/29395082/kinetic-profiles-of-photocurrents-in-cells-expressing-two-types-of-channelrhodopsin-genes
#10
Yoshito Watanabe, Eriko Sugano, Kitako Tabata, Taku Ozaki, Takehiko Saito, Makoto Tamai, Hiroshi Tomita
Channelrhodopsin-2 (ChR2), a light-activated cation-selective ion channel, has been widely used as a tool in optogenetic research. ChR2 is specifically sensitive to wavelengths less than 550 nm. One of the methods to expand the sensitivity of a channelrhodopsin to a wider range of wavelengths is to express another channelrhodopsin in the cells by the transduction of an additional gene. Here, we report the characteristic features of cells expressing two types of channelrhodopsins, each having different wavelength sensitivities...
January 29, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29394759/-a-case-of-pathological-complete-response-following-neoadjuvant-chemotherapy-with-gemcitabine-plus-nab-paclitaxel-in-borderline-resectable-pancreatic-cancer
#11
Noriyuki Ohno, Makoto Ishida, Kenji Ohnishi, Katsuji Sawai, Shinsuke Tabata, Masaaki Deguchi, Tatsumi Kitajima, Yasuharu Kaizaki
We report a resected case with a pathological complete response(pCR)after neoadjuvant chemotherapy for borderline resectable pancreatic cancer(BRPC). A 67-year-old woman who had been treated for type 2 diabetes mellitus in our hospital presented with an exacerbation of diabetes. An abdominal CT scan confirmed a hypovascular mass in the pancreas body consistent with BRPC. After 3 courses of chemotherapy with gemcitabine plus nab-paclitaxel(GnP), her elevated DUPAN-2 level normalized. A follow up CT scan revealed that the tumor had decreased in size, and no distant metastasis was detected...
November 2017: Gan to Kagaku Ryoho. Cancer & Chemotherapy
https://www.readbyqxmd.com/read/29387637/three-dimensional-architecture-of-the-ligamentum-teres-in-the-human-hip-joint
#12
Nobuhiro Kaku, Tatsuo Shimada, Tomonori Tabata, Hiroaki Tagomori, Tetsutaro Abe, Juan Juan Zhang, Hiroshi Tsumura
Background: We aimed to investigate the three-dimensional structure of the collagenous fibers of the ligamentum teres (LT) of the human hip and clarify the LT micro-anatomy at the attachment of the femoral head. Methods: Femoral heads and LT were collected during hip arthroplasty. Specimens were cut into 5-10-mm squares, prepared, developed, and observed under a light microscope. Next, specimens were prepared and examined under a scanning electron microscope (SEM)...
July 2017: Muscles, Ligaments and Tendons Journal
https://www.readbyqxmd.com/read/29386539/met-or-nras-amplification-is-an-acquired-resistance-mechanism-to-the-third-generation-egfr-inhibitor-naquotinib
#13
Kiichiro Ninomiya, Kadoaki Ohashi, Go Makimoto, Shuta Tomida, Hisao Higo, Hiroe Kayatani, Takashi Ninomiya, Toshio Kubo, Eiki Ichihara, Katsuyuki Hotta, Masahiro Tabata, Yoshinobu Maeda, Katsuyuki Kiura
As a third-generation epidermal growth factor receptor (EGFR) tyrosine kinase inhibitor (TKI), osimeritnib is the standard treatment for patients with non-small cell lung cancer harboring the EGFR T790M mutation; however, acquired resistance inevitably develops. Therefore, a next-generation treatment strategy is warranted in the osimertinib era. We investigated the mechanism of resistance to a novel EGFR-TKI, naquotinib, with the goal of developing a novel treatment strategy. We established multiple naquotinib-resistant cell lines or osimertinib-resistant cells, two of which were derived from EGFR-TKI-naïve cells; the others were derived from gefitinib- or afatinib-resistant cells harboring EGFR T790M...
January 31, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29381650/effects-of-different-exercise-modes-on-arterial-stiffness-and-nitric-oxide-synthesis
#14
Natsuki Hasegawa, Shumpei Fujie, Naoki Horii, Eri Miyamoto-Mikami, Katsunori Tsuji, Masataka Uchida, Takafumi Hamaoka, Izumi Tabata, Motoyuki Iemitsu
PURPOSE: Aerobic training (AT) and high-intensity intermittent training (HIIT) reduce arterial stiffness, whereas resistance training (RT) induces deterioration of or no change in arterial stiffness. However, the molecular mechanism of these effects of different exercise modes remains unclear. This study aimed to clarify the difference of different exercise effects on endothelial nitric oxide synthase (eNOS) signaling pathway and arterial stiffness in rats and humans. METHODS: In the animal study, forty 10-week-old male Sprague-Dawley rats were randomly divided into 4groups: sedentary control (CON), AT (treadmill running, 60min at 30m/min, 5days/wk for 8weeks), RT (ladder-climbing, 8-10sets/day, 3days/wk for 8weeks), and HIIT (14repeats of 20-sec swimming session with 10-sec pause between sessions, 4days/wk for 6weeks from 12-week-old) groups (n=10 in each group)...
January 30, 2018: Medicine and Science in Sports and Exercise
https://www.readbyqxmd.com/read/29372398/transapical-approach-in-transcatheter-cardiovascular-interventions
#15
REVIEW
Minoru Tabata
The left ventricular apex has excellent accessibility to the aortic valve, mitral valve, left ventricular outflow tract and thoracic aorta. Although the number of transapical approach in transcatheter aortic valve replacement has been decreasing in recent years, it is still a useful option for patients with very poor peripheral vascular access. The apex has been chosen as a primary access site for many devices of transcatheter mitral valve repair/replacement and mitral valve-in-valve procedures. Additionally, the transapical approach has been used for other transcatheter cardiovascular interventions such as paravalvular leak repair after mitral or aortic valve replacement, pseudoaneurysm repair of the left ventricular outflow tract, and thoracic endovascular aortic repair...
January 25, 2018: General Thoracic and Cardiovascular Surgery
https://www.readbyqxmd.com/read/29370114/acidic-chitinase-chitin-complex-is-dissociated-in-a-competitive-manner-by-acetic-acid-purification-of-natural-enzyme-for-supplementation-purposes
#16
Eri Tabata, Akinori Kashimura, Satoshi Wakita, Masayoshi Sakaguchi, Yasusato Sugahara, Yasutada Imamura, Hideaki Shimizu, Vaclav Matoska, Peter O Bauer, Fumitaka Oyama
Acidic chitinase (Chia) has been implicated in asthma, allergic inflammations, and food processing. We have purified Chia enzymes with striking acid stability and protease resistance from chicken and pig stomach tissues using a chitin column and 8 M urea (urea-Chia). Here, we report that acetic acid is a suitable agent for native Chia purification from the stomach tissues using a chitin column (acetic acid-Chia). Chia protein can be eluted from a chitin column using 0.1 M acetic acid (pH 2.8), but not by using Gly-HCl (pH 2...
January 25, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29368530/physiological-perceptual-and-affective-responses-to-six-high-intensity-interval-training-protocols
#17
Lucio Follador, Ragami C Alves, Sandro Dos S Ferreira, Cosme F Buzzachera, Vinicius F Dos S Andrade, Erick D S de A Garcia, Raul Osiecki, Sara C Barbosa, Letícia M de Oliveira, Sergio G da Silva
This study examined the extent to which different high-intensity interval training (HIIT) and sprint interval training (SIT) protocols could influence psychophysiological responses in moderately active young men. Fourteen participants completed, in a randomized order, three cycling protocols (SIT: 4 × 30-second all-out sprints; Tabata: 7 × 20 seconds at 170% ⋮O2max; and HIIT: 10 × 60 seconds at 90% HRmax) and three running HIIT protocols (4 × 4 minutes at 90%-95% HRmax, 5 × at v⋮O2max, and 4 × 1,000 meters at a rating of perceived exertion (RPE) of 8, from the OMNI-Walk/Run scale)...
January 1, 2018: Perceptual and Motor Skills
https://www.readbyqxmd.com/read/29363594/a-virus-targeted-plant-receptor-like-kinase-promotes-cell-to-cell-spread-of-rnai
#18
Tabata Rosas-Diaz, Dan Zhang, Pengfei Fan, Liping Wang, Xue Ding, Yuli Jiang, Tamara Jimenez-Gongora, Laura Medina-Puche, Xinyan Zhao, Zhengyan Feng, Guiping Zhang, Xiaokun Liu, Eduardo R Bejarano, Li Tan, Heng Zhang, Jian-Kang Zhu, Weiman Xing, Christine Faulkner, Shingo Nagawa, Rosa Lozano-Duran
RNA interference (RNAi) in plants can move from cell to cell, allowing for systemic spread of an antiviral immune response. How this cell-to-cell spread of silencing is regulated is currently unknown. Here, we describe that the C4 protein from Tomato yellow leaf curl virus can inhibit the intercellular spread of RNAi. Using this viral protein as a probe, we have identified the receptor-like kinase (RLK) BARELY ANY MERISTEM 1 (BAM1) as a positive regulator of the cell-to-cell movement of RNAi, and determined that BAM1 and its closest homolog, BAM2, play a redundant role in this process...
January 23, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29362395/chitin-digestibility-is-dependent-on-feeding-behaviors-which-determine-acidic-chitinase-mrna-levels-in-mammalian-and-poultry-stomachs
#19
Eri Tabata, Akinori Kashimura, Azusa Kikuchi, Hiromasa Masuda, Ryo Miyahara, Yusuke Hiruma, Satoshi Wakita, Misa Ohno, Masayoshi Sakaguchi, Yasusato Sugahara, Vaclav Matoska, Peter O Bauer, Fumitaka Oyama
Chitin, a polymer of N-acetyl-D-glucosamine (GlcNAc), functions as a major structural component in chitin-containing organism including crustaceans, insects and fungi. Recently, we reported that acidic chitinase (Chia) is highly expressed in mouse, chicken and pig stomach tissues and that it can digest chitin in the respective gastrointestinal tracts (GIT). In this study, we focus on major livestock and domestic animals and show that the levels of Chia mRNA in their stomach tissues are governed by the feeding behavior...
January 23, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29362338/-a-resected-case-of-ur-la-pancreatic-tail-cancer-with-aortic-invasion-after-chemoradiotherapy
#20
Yu Fujimura, Masami Tabata, Tatsuya Sakamoto, Koki Maeda, Ichiro Ohsawa, Kenji Kato, Makoto Iwata, Takayuki Sanda
A 70-year-old male was referred to our hospital because of weight loss and epigastric discomfort. CT showed an irregularshaped, low-density tumor, 12 cm in diameter in the tail of the pancreas. This tumor widely invaded to the left kidney and to the anterior and left lateral sides of the aorta in spite of no involvement of celiac and superior mesenteric arteries. Moreover, it closely contacted with the stomach and the spleen. EUS-fine-needle aspiration biopsy of the tumor detected adenocarcinoma. Thus, he was diagnosed with UR-LA pancreatic cancer with aortic invasion...
January 2018: Gan to Kagaku Ryoho. Cancer & Chemotherapy
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